Food Science & Nutrition最新文献

{"title":"Functional Evaluation of Cooking-Mimicking Extracts From Chinese Olive (Canarium album L.) Leaves, Fruits, and Pits Using Cell-Based and In Silico Analysis","authors":"Chun-Wai Chan,&nbsp;Yu-Jo Tsai,&nbsp;Ting-Jang Lu,&nbsp;Yi-Chun Liao,&nbsp;Shu-Chen Hsieh","doi":"10.1002/fsn3.70337","DOIUrl":"https://doi.org/10.1002/fsn3.70337","url":null,"abstract":"<p>Chinese olive (CO; <i>Canarium album</i> L.) fruits are rich in phytochemicals with known physiological benefits. However, most studies evaluating the effects of CO have used ethyl acetate, acetone, and chloroform as the extraction solvents, which do not accurately reflect real dietary conditions. Moreover, research on CO leaves and pits remains limited. This study investigated the functional properties of extracts derived from CO leaves, fruits, and pits, prepared under simulated conventional culinary and dietary conditions (including solvents, extract combinations, and heat treatment), referred to as cooking-mimicking extracts. Our results revealed that most CO extracts reduced the production of nitric oxide in lipopolysaccharide-stimulated RAW264.7 macrophages and inhibited the proliferation of HCT116 colorectal cancer cells. Combinations of water-soluble CO extracts exerted a synergistic effect, suppressing cancer cell proliferation. To the best of our knowledge, this study is the first to identify methylellagic acid deoxyhexoside, vitexin 2-<i>O</i>-rhamnoside, and isovitexin 2-<i>O</i>-rhamnoside in CO extracts. Molecular docking predicted strong interactions with phosphofructokinase (PFK), a key glycolytic enzyme implicated in cancer cell proliferation. Treatment with CO extracts, including heat-treated forms, markedly reduced PFK activity and cell viability in HCT116 cells. These findings provide new insights into the bioactive constituents and physiological functions of CO extracts, supporting their potential application in dietotherapy.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70337","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Diet to Scar: Novel Mendelian Randomization and Mediation Analyses Linking Dietary Habits, Gut Microbiota, and Hypertrophic Scarring","authors":"Qiong Liu,&nbsp;Xiaofang Liu,&nbsp;Mengge Gao,&nbsp;Bo Yang,&nbsp;Miaoqing Luo,&nbsp;Biying Yang,&nbsp;Guojun Liang","doi":"10.1002/fsn3.70292","DOIUrl":"https://doi.org/10.1002/fsn3.70292","url":null,"abstract":"<p>Hypertrophic scarring (HTS) is a pathological skin condition characterized by excessive collagen deposition during wound healing. Emerging evidence suggests that dietary habits and gut microbiota composition may influence HTS risk via systemic inflammatory and metabolic pathways. However, the causal relationships between these factors remain poorly understood. Mendelian randomization (MR) analysis was conducted to investigate the causal relationships between dietary habits, gut microbiota composition, and HTS risk. Additional analyses included mediation analysis to explore potential intermediary effects of gut microbiota and co-localization analysis to assess shared genetic loci between exposures and HTS. MR analysis identified significant associations between HTS and six dietary preferences, with caffeinated/sweet liking and jam liking increasing HTS risk, while crisps, curry, oranges, and strong flavor liking were protective. For gut microbiota, <i>Eubacterium coprostanoligenes</i>, Collinsella, and Coprococcus1 showed protective effects, whereas Adlercreutzia was positively associated with HTS risk. Mediation analysis did not support gut microbiota as a significant mediator between dietary habits and HTS, and co-localization analysis indicated distinct genetic determinants for these traits. The study highlights the independent roles of dietary habits and gut microbiota in influencing HTS risk, suggesting potential dietary and microbial-targeted interventions for scar prevention. Further research in diverse populations is needed to validate these findings and explore their clinical applications.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70292","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influencing Factors of Chinese Consumers' Acceptance of Irradiated Food","authors":"Gang Li,&nbsp;Zhengkui Zeng,&nbsp;Ke Wang","doi":"10.1002/fsn3.70353","DOIUrl":"https://doi.org/10.1002/fsn3.70353","url":null,"abstract":"<p>Food irradiation enhances food safety and shelf life, but consumer acceptance in China is hindered by radiation-related misconceptions and low awareness. This study investigated 556 Chinese consumers through an online survey, analyzing demographic influences and attitudes via SPSSAU. The key findings revealed a strong preference for the term “ionized food” (69% acceptance) over “irradiated food” (31%), particularly among those with nuclear risk concerns. This finding highlights the notion that the use of the label “ionized food” can avoid knee-jerk associations with nuclear radiation. When the term “irradiated food” is employed, clear explanations of what irradiated food involves should be provided on food labels. While over 80% of the respondents recognized conventional sterilization methods (e.g., pasteurization), only 17% identified irradiation. Demographic analysis showed higher initial acceptance among men and the 26–45 age group (75% willingness), although disclosure of everyday irradiated products (e.g., spices, snacks) triggered resistance, especially among women (77% of reversals). Education positively correlated with acceptance, with doctoral students showing 64.5% approval. Notably, 65.6% of initially willing consumers rejected irradiated foods after learning about inconspicuous labeling, reflecting distrust in market transparency. Mobile platforms (WeChat, TikTok) were preferred for science communication (70%), while older adults favored community outreach. Targeted science communication campaigns should be launched to dispel radiation-related misconceptions. Governments should also customize strategies for different demographic groups, particularly to address the concerns of students, women, and elderly individuals. By leveraging digital media and collaborating with schools, communities, and businesses, consumers can be effectively educated, their concerns can be alleviated, and trust in irradiated food can be enhanced.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70353","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Simple Protocol to Assess Glucose Release After In Vitro Digestion, Allowing Comparison of Starchy Foods","authors":"Thomas Montebugnoli,&nbsp;Giorgia Antonelli,&nbsp;Elena Chiarello,&nbsp;Nicholas S. Rivera,&nbsp;Giulia Camporesi,&nbsp;Francesca Danesi,&nbsp;Alessandra Bordoni","doi":"10.1002/fsn3.70323","DOIUrl":"https://doi.org/10.1002/fsn3.70323","url":null,"abstract":"<p>Diets including foods with low glycemic index have been proposed to reverse the rising incidence of obesity and type 2 diabetes. Unfortunately, many common starchy staple foods have a high glycemic index. Several strategies for producing foods with low glycemic index have been proposed, but their application by the food industry is limited by the difficulty and cost of measuring GI in vivo. The aim of the present study was to develop a simple and reliable protocol to assess glucose release after in vitro digestion, and to use it to compare commercial starchy foods. To this purpose, a final starch digestion step was included in the INFOGEST in vitro digestion protocol by adding the enzyme amyloglucosidase, which mimicked the action of brush border enzymes. Glucose release was evaluated in five starchy foods (flour, bread, crackers, and <i>durum</i> wheat pasta and gluten-free pasta), available on the market in two forms produced by the same company, one standard and one rich in fiber (&gt; 6 g/100 g). Glucose release was evaluated 0, 30, 60, and 120 min after the addition of amyloglucosidase. In all foods, glucose release at T0 was very low, confirming that starch digestion by amylases is incomplete. Excluding flours, the lowest and highest amounts of glucose release (28.29 and 49.36 g/50 g of available carbohydrates) were detected in <i>durum</i> wheat pasta and gluten-free pasta, respectively, confirming the high glycemic index previously detected in many gluten-free products. Notably, when glucose release was expressed as g/50 g of available carbohydrate, the impact of fiber content was negligible. Although the in vitro assessment of glucose release should not be confused with the glycemic index, the herein reported protocol can help predict the impact of foods on glycemia, facilitating the formulation of healthier products.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70323","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Hyperlipidemic and Anti-Atherogenic Effect of Citrus Peel Pectin Against Cholesterol and Cholic Acid Induced Hyperlipidemia in Sprague Dawley Rats","authors":"Hina Rasheed,&nbsp;Ammar B. Altemimi,&nbsp;Roshina Rabail,&nbsp;Sidra Tul Muntaha,&nbsp;Allah Rakha,&nbsp;Usman Haider,&nbsp;Fareha Rasheed,&nbsp;Maham Shehzad,&nbsp;Amin Mousavi Khaneghah,&nbsp;Gholamreza Abdi,&nbsp;Rana Muhammad Aadil","doi":"10.1002/fsn3.70274","DOIUrl":"https://doi.org/10.1002/fsn3.70274","url":null,"abstract":"<p>This study was designed to explore the anti-hyperlipidemic and anti-atherogenic effects of citrus peel-derived pectin. A total of 70 Sprague Dawley rats were categorized into 7 groups with 10 rats in each group. Hyperlipidemia was induced in all groups by adding cholesterol (1.5%) and cholic acid (0.5%) to the normal diet for 15 days. G₀ was considered a normal control, G₁ was considered a negative control, G₂ was considered the positive control treated with the standard drug atorvastatin (10 mg/kg p.o.), G₃, G₄, G₅, and G₆ were given 3%, 7%, 11%, and 15% of citrus peel pectin, respectively, for 60 days. After the trial completion, biochemical markers like lipid profile, liver and renal function tests, and atherogenic index as well as histopathological analysis of heart, liver, and kidney were performed. The results showed a significant decline in TC, TG, and LDL, while significantly increased serum HDL levels consequently reduced the atherogenic index (<i>p</i> &lt; 0.05). The TG levels were positively correlated with the atherogenic index, whereas HDL levels were negatively correlated with the atherogenic index. The outcome further suggested that the treatment with citrus peel pectin (15%) significantly enhanced the antioxidant levels and restored the liver and renal function as evidenced by the histopathological studies. Additionally, the findings revealed a significant reduction in the pathological changes of heart tissue treated with citrus peel pectin at different ratios. The highest reduction was seen in G₆ (treated with 15% citrus peel pectin). As a result of pectin's safety and considerable anti-hyperlipidemic, anti-atherogenic, and antioxidant effects, it could be evaluated as a therapeutic option for hyperlipidemia. Thus, citrus peel pectin can be used as an adjunct functional food in the diet to manage hyperlipidemia and improve atherogenesis.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70274","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of α-Linolenic Acid on the Intestinal Barrier Integrity and Intestinal Antioxidant Status in Broilers","authors":"Ao Kang,&nbsp;Jialei Ni,&nbsp;Xinyu Cheng,&nbsp;Shuyu Wu,&nbsp;Yun Liu,&nbsp;Weiming Ma,&nbsp;Dong Wang","doi":"10.1002/fsn3.70271","DOIUrl":"https://doi.org/10.1002/fsn3.70271","url":null,"abstract":"<p>This study aimed to investigate the beneficial effects of α-linolenic acid (ALA) on intestinal barrier function and antioxidant status in broilers, along with the associated molecular mechanisms. 320 one-day-old Arbor Acres broilers were randomly divided into four groups, each with eight replicates, and fed diets with 0 (control), 200, 400, and 600 mg of ALA/kg for 42 days. ALA supplementation did not significantly affect the broilers' overall growth performance. Supplementing diets with 400 and 600 mg/kg of ALA significantly enhanced (<i>p</i> &lt; 0.05) jejunal and ileal villus height, the jejunal villus height to crypt depth ratio, and ileal mRNA expression and protein levels of Zonula occludens-1 (ZO-1) and occludin in broilers on Day 42. Broilers fed diets containing 600 mg/kg of ALA exhibited significantly increased (<i>p</i> &lt; 0.05) serum catalase (CAT) activity, total antioxidant capacity (T-AOC), and jejunal and ileal activities of CAT and total superoxide dismutase (T-SOD), alongside reduced malondialdehyde (MDA) concentrations in serum, jejunum, and ileum on Days 21 and 42, compared to the control group. Supplementing 600 mg/kg of ALA significantly increased (<i>p</i> &lt; 0.05) the mRNA expressions of CAT, SOD1, NRF2, and HO-1, along with the protein levels of cytoplasmic and nuclear NRF2 and HO-1 in the jejunum and ileum on Days 21 and 42. These findings demonstrate the protective effects of ALA in improving intestinal health in broilers. The underlying mechanisms may involve enhancing intestinal barrier integrity by increasing tight junction protein abundance and boosting intestinal antioxidant capacity by elevating antioxidant enzyme activity and activating the NRF2 pathway. In conclusion, our results showed that 600 mg/kg of ALA was identified as the optimal concentration for improving intestinal barrier function and antioxidant status in broilers, highlighting its potential for protecting intestinal health through ALA-based interventions.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70271","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Bacterial Diversity in Jersey Cow Colostrum and Mature Milk and the Study of the Probiotic Functions of Ligilactobacillus salivarius CR29","authors":"Qibin Wang,&nbsp;Jianing Xu,&nbsp;Lichun Shang,&nbsp;Qingshen Sun","doi":"10.1002/fsn3.70325","DOIUrl":"https://doi.org/10.1002/fsn3.70325","url":null,"abstract":"<p>This study used 16S rDNA high-throughput sequencing to analyze the bacterial diversity of Jersey cow colostrum and mature milk, and evaluated the probiotic properties of <i>Ligilactobacillus salivarius</i> CR29 isolated from the samples. The bacterial community structure of the Jersey cow milk samples was analyzed, and lactic acid bacteria were isolated using MRS medium. The potential probiotic strains were evaluated for growth, acid production, hemolytic activity, antibacterial activity, antibiotic sensitivity, acid and bile tolerance, hydrophobicity, autoaggregation and coaggregation abilities, as well as their survival in a simulated gastrointestinal environment. The results showed that nine lactic acid bacterial strains isolated from the milk samples exhibited probiotic potential, among which <i>Ligilactobacillus salivarius</i> CR29 showed no hemolysis and had strong antibacterial activity, significantly inhibiting several pathogens compared to other strains. CR29 was sensitive to multiple antibiotics (such as tetracycline and rifampicin), and its survival rates under 0.3% bile salt and pH 2 conditions were 56.28% and 77.59%, respectively. Its survival rate after simulated gastrointestinal passage was 47.86%. In summary, <i>Ligilactobacillus salivarius</i> CR29 demonstrated excellent probiotic potential and may be applied in functional foods and health-related fields in the future.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70325","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between ω-3, ω-6 Polyunsaturated Fatty Acid and Sleep Disorders: From Cross-Sectional to Mendelian Randomization Studies","authors":"Lin Wang,&nbsp;Wei Quan,&nbsp;Jia Song,&nbsp;Yidan Qin,&nbsp;Huibin Zeng,&nbsp;Jian Zhang,&nbsp;Xuan Zhao,&nbsp;Jia Li,&nbsp;Jiajun Chen","doi":"10.1002/fsn3.70311","DOIUrl":"https://doi.org/10.1002/fsn3.70311","url":null,"abstract":"<p>Sleep disorders are a common health problem affecting a significant proportion of the adult population. Emerging evidence suggests that dietary factors, particularly polyunsaturated fatty acid (PUFA) intake, may play a role in modulating sleep quality. This study aims to investigate the association between omega-3 (ω-3) and omega-6 (ω-6) PUFA and sleep disorders using cross-sectional survey data and data from genome-wide association studies (GWAS). Using data from the National Health and Nutrition Examination Survey (NHANES, 2005–2018), we analyzed a cohort of 31,920 participants, with the primary independent variables being intake of ω-3 and ω-6 PUFAs. Multivariate regression was used to assess associations, and restricted cubic spline analysis was used to explore potential non-linear dose–response relationships. Two-sample Mendelian randomization (MR) analyses were performed to evaluate the causal effects of levels of multiple fatty acids on the risk of sleep disorders. For analysis on NHANES data, the participants with sleep disorders had significantly lower ω-3 PUFA intake (1.71 ± 1.11 g) compared to those without sleep disorders (1.78 ± 1.14 g, <i>p</i> &lt; 0.001). Regression analysis revealed that higher ω-3 PUFA intake was associated with a reduced risk of sleep disorders, while the MR analyses showed that a higher ratio of ω-6 to total fatty acid levels was causally associated with a lower risk of sleep disorders (IVW OR = 0.930, 95% CI: 0.880–0.983, <i>p</i> = 0.011). Our findings suggest that increased ω-3 FA intake and increased ratio of ω-6 to total fatty acid level may be associated with a lower risk of sleep disorders, highlighting the potential benefits of dietary modification for sleep health. Future research should further explore these associations and consider intervention studies to establish causality and optimal dietary recommendations to prevent sleep disorders.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70311","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Oxidation Detection in White Wine by Electronic Tongue: A Preliminary Study","authors":"Rachel I. Potter,&nbsp;Jungmin Lee,&nbsp;Carolyn F. Ross","doi":"10.1002/fsn3.70366","DOIUrl":"https://doi.org/10.1002/fsn3.70366","url":null,"abstract":"<p>In white wines, early detection of oxidation would alert winemakers to monitor potentially troubled wine more closely and take preventative measures to mitigate undesirable browning, flavors, and odors in their products. Current early oxidation detection methods include assessment by browning index, trained sensory panels, and quantification of byproducts such as quinones. The objective of this study was to assess the capability of the e-tongue, a fairly new instrument that has previously been used to detect wine faults caused by spoilage organisms, in detecting early oxidative changes in Chardonnay wine. Clear bottles of Chardonnay were stored partially opened (treatment) in the dark at 2.2°C for 24 weeks. Wines were assessed at seven time intervals (0, 1, 2, 4, 8, 16, and 24 weeks) using the e-tongue and a semi-trained sensory panel with rate-all-that-apply descriptors. Beginning at week 8 of storage, the e-tongue discrimination indices (DI) between control and treated wine (sealed wine stored alongside partially opened wine bottles) were high (DI &gt; 80%) and remained high throughout the study, indicating that the e-tongue distinguished between control and treated samples. However, sensory panelists detected an increase in the intensity of vinegar/nail polish remover aroma attributes, attributes associated with wine oxidation, after 16 weeks of storage. These results suggest that the e-tongue is a useful tool in the early detection of oxidized wine samples as compared to a sensory panel that perceived differences between control and treated wines 8 weeks after differences were detected by the e-tongue.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70366","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nephroprotective Impacts of Hyphaene thebaica (Doum) Against Gentamicin-Induced Renal Toxicity Through Different Signaling Pathways","authors":"Saed A. Althobaiti","doi":"10.1002/fsn3.70345","DOIUrl":"https://doi.org/10.1002/fsn3.70345","url":null,"abstract":"<p><i>Hayphaene thebaica</i> (<i>H. thebaica</i>) is thought to be effective in treating several diseases and has possible anti-toxic impacts. Gentamycin (GM) toxicity in internal organs is mostly induced by oxidative stress production. Therefore, the current study aimed to examine the protective effect of <i>H. thebaica</i>) against gentamycin-induced renal toxicity. Twenty-four male mice were allocated into four groups: first group administered saline orally as a control, the second group administered <i>H. thebaica</i> extract (5%) for 2 weeks, third group injected intraperitoneally with GM (100 mg/kg, daily) for 15 days, and fourth group served as the protective one, receiving doses used in second and third groups for 2 weeks. To examine kidney function, serum was extracted. Renal tissues were collected and examined for gene expression, histopathology, and immunohistochemistry for nuclear factor kappa B (NF_kB), transforming growth factor beta-1 (TGF-β1), and tumor necrosis factor-alpha (TNF-α) immunoreactivity. Serum levels of creatinine, uric acid, and urea were all increased after gentamicin injection. TGF-β1 and cyclooxygenase-2 (COX2) were upregulated in renal tissues of the gentamycin group compared to the control and <i>H. thebaica</i> groups using real-time quantitative PCR (RT-qPCR) analysis, unlike genes of oxidative stress markers, nuclear factor erythroid 2-related factor 2 (Nrf2) was downregulated and was ameliorated by <i>H. thebaica</i> pre-administration. Immunoreactivity of genes of TGF-β1, NF_kB, and TNF-α was significantly immunoreacted and expressed in GM-injected mice and was normalized and ameliorated in the protective group. There were deformities in renal histology represented by degenerative changes in the renal glomerulus, which was surrounded by a wide capsular space, degeneration in the tubular epithelium, and an increase in the accumulation of acidophilic proteinaceous substances within the lumens of some tubules in the gentamycin-injected group compared to the protective group. These deformities were ameliorated by <i>Hyphaene thebaica</i> administration in the protective group.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70345","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}