{"title":"Bioactive properties of peptide fractions from Brazilian soy protein hydrolysates: In silico evaluation and experimental evidence","authors":"T.C. Farias , J.P. Abreu , J.P.S. Oliveira , A.F. Macedo , A Rodríguez-Vega , A.P. Tonin , F.S.N. Cardoso , E.C. Meurer , M.G.B. Koblitz","doi":"10.1016/j.fhfh.2022.100112","DOIUrl":"10.1016/j.fhfh.2022.100112","url":null,"abstract":"<div><p>Soybeans are a known source of dietary proteins and potential bioactive peptides. In this study, a protein hydrolysate from soybean protein concentrate was produced using papain. The peptides were separated by ultrafiltration (< and > 3 kDa, LMMH (low molecular mass) and HMMH (high molecular mass), respectively) and sequenced through LC-MS/MS. To obtain a thorough identification of the peptides in the hydrolysate, different analysis methods and bioinformatics techniques were applied, covering a molecular mass range from more than 480 Da up to small dipeptides. The antioxidant and the inhibitory α -glucosidase and lipase potentials were evaluated by different <em>in vitro</em> tests. Sixty-nine peptides were identified in the HMMH fraction and 32 in LMMH, but only 16 matched the 118 sequences obtained by <em>in silico</em> simulated hydrolysis. Unlike previous reports, the HMMH fraction showed higher antioxidant activity, by all 5 <em>in vitro</em> methods applied, which was not accompanied by the <em>in silico</em> evaluation. Both high and low molecular mass fractions showed similar inhibitory activities against α -glucosidase and pancreatic lipase. LMMH, however, showed better results for α -glucosidase inhibition (IC50 = 0,94), in agreement with the <em>in silico</em> evaluation. This combination of bioactivities makes the fractions of this hydrolysate potential food ingredients with the possible ability to delay the lipid peroxidation of meat products, limiting the digestion of lipids in the product and also with the potential to delay the digestion of carbohydrates ingested in the same meal.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48102975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shiyuan Mao , Lovedeep Kaur , Tai-Hua Mu , Jaspreet Singh
{"title":"Preparation and characterisation of plant and dairy-based high protein Chinese steamed breads (mantou): Microstructural characteristics and gastro-small intestinal starch digestion in vitro","authors":"Shiyuan Mao , Lovedeep Kaur , Tai-Hua Mu , Jaspreet Singh","doi":"10.1016/j.fhfh.2022.100111","DOIUrl":"10.1016/j.fhfh.2022.100111","url":null,"abstract":"<div><p>The effects of dairy and plant protein addition on microstructural characteristics and <em>in vitro</em> gastro-small intestinal starch digestion characteristics of Chinese steamed breads (CSBs) were studied. Breads containing rennet casein (RC) and a mixture of soy protein isolate and milk protein concentrate (SM) at two different levels (RC I, RC II; SM I, SM II) were prepared. Microstructural characteristics of the undigested and digested control (100% wheat flour) bread and high protein steam bread (HPCSB) versions were compared through scanning electron microscopy. The compact microstructure of HPCSBs displayed a network of proteins wrapped around starch granules and had fewer air cells compared to the control. The addition of both proteins influenced the microstructure of HPCSBs, which in turn affected their textural and starch digestion properties. The <em>in vitro</em> starch digestion of control CSB and HPCSBs confirmed that the addition of proteins is capable of lowering the starch hydrolysis (%). The highest starch hydrolysis was observed for the control wheat bread, followed by SM1 > RC I > SM II and RC II at the end of the small-intestinal digestion. The estimated glycaemic indices (eGI) for all HPCSBs were statistically lower than the control CSB. In comparison to control CSB, the microstructure of HPCSBs appeared more irregular, less porous, and compact during gastric and small intestinal digestion.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46704320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nemany A.N. Hanafy , Elsayed I. Salim , Magdy E. Mahfouz , Eman A. Eltonouby , Izar H. Hamed
{"title":"Fabrication and characterization of bee pollen extract nanoparticles: Their potential in combination therapy against human A549 lung cancer cells","authors":"Nemany A.N. Hanafy , Elsayed I. Salim , Magdy E. Mahfouz , Eman A. Eltonouby , Izar H. Hamed","doi":"10.1016/j.fhfh.2022.100110","DOIUrl":"10.1016/j.fhfh.2022.100110","url":null,"abstract":"<div><p>Bee pollen extract (BPE) based polymer nanoparticles (BPENP) were fabricated in the current study by using bovine serum albumin (BSA) and the complex was then coated by folic acid conjugated protamine to be targeted for cancer cells. Spectroscopic and microscopic methods were used to characterize the resultant nanoparticles. High flavonoid and polyphenolic contents were detected by HPLC. At 336 nm, it was found that BPENP provided the highest absorption by UV. The average particle size ranged between 25 and 40 nm. The inhibitory effects and IC50 values of the acquired BPE, BPENP, Avastin, or their combination at 24 and 48 h were detected against A549 lung cancer cells. The combination therapy assay of BPENP and Avastin revealed a very significant synergism between the two drugs with a low combination index (CI). These therapies particularly the combination therapy have significantly affected the expression of the <em>HRAS, MAPK,</em> and apoptotic genes such as <em>Bax, Bcl-2,</em> and <em>Caspase 3</em>. It was established that obtained BPENP might be employed to support the administration of Avastin for the treatment of lung cancer.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45414487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Food colloid-based delivery systems for tackling age-related macular degeneration by enhancing carotenoid bioavailability: A review","authors":"Erin Kuo , David Julian McClements","doi":"10.1016/j.fhfh.2022.100093","DOIUrl":"10.1016/j.fhfh.2022.100093","url":null,"abstract":"<div><p>Over the past century, there has been a large increase in the life expectancy of people around the globe, which means there has been a rise in diseases of the elderly. Age-related macular degeneration (AMD) is a sight-threatening condition of the eye characterized by a loss of central vision. Research suggests that macular pigment carotenoids, such as lutein and zeaxanthin, may inhibit the onset of this disease by protecting the eye from damaging light and oxidation. These carotenoids are chemically reactive hydrophobic molecules with a low water-solubility, chemical stability, and bioavailability. Consequently, many carotenoid-rich foods and supplements are not absorbed by the human body and do not exhibit their beneficial effects on eye health. In this article, we review the prevalence and characteristics of AMD, the sources of macular pigment carotenoids in foods, the factors limiting their bioavailability, and analytical approaches available to study their bioavailability and bioactivity. We then discuss different strategies for increasing the concentration of bioavailable macular pigment carotenoids in the human diet, including dietary sources, supplements, functional foods, and excipient foods, with an emphasis on colloidal systems that can be used for this purpose.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667025922000401/pdfft?md5=d1b126d9927ef6f5a1ed0d2d698e8bc7&pid=1-s2.0-S2667025922000401-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49318490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carolina Madazio Niro , Jackson Andson de Medeiros , Joana Dias Bresolin , Ana Paula Dionísio , Mateus Kawata Salgaço , Katia Sivieri , Henriette M.C. Azeredo
{"title":"Banana leathers as influenced by polysaccharide matrix and probiotic bacteria","authors":"Carolina Madazio Niro , Jackson Andson de Medeiros , Joana Dias Bresolin , Ana Paula Dionísio , Mateus Kawata Salgaço , Katia Sivieri , Henriette M.C. Azeredo","doi":"10.1016/j.fhfh.2022.100081","DOIUrl":"10.1016/j.fhfh.2022.100081","url":null,"abstract":"<div><p>Concerns abouth diet-health relationships have led many people to include healthier snacks in their diets, including those with functional (including probiotic) properties. This study was focused on development of probiotic-loaded banana leathers. Two probiotic bacteria (the spore-forming <em>Bacillus coagulans</em> and the conventional non-spore-forming <em>Lactobacillus acidophilus</em>) and two polymeric matrices (digestible cassava starch and non-digestible bacterial cellulose - BC) have been used. The presence of probiotic bacteria (mainly L. <em>acidophilus</em>) reduced the tensile strength, elastic modulus and shear force of the leathers, while the BC-based leathers were stronger, stiffer and more resistant to shear stress than the starch-based ones. While a high probiotic viability was kept on fruit leathers loaded with <em>B. coagulans</em> during drying and room-temperature storage, those loaded with L. <em>acidophilus</em> suffered high viability losses upon drying, which was ascribed to osmotic stress. The nature of the biopolymeric matrix has not significantly influence the bacterial viability losses along processing and storage, or the final viable cell count released into the intestine (as assessed using an INFOGEST static in vitro simulated digestion model). The banana leathers loaded with <em>B. coagulans</em> were well accepted, irrespectively of being produced from BC or starch, although some negative comments on the texture and flavor of the BC-based ones have been more frequent than with the starch-based ones.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667025922000280/pdfft?md5=a9feaeb0454a935520c277b2da352074&pid=1-s2.0-S2667025922000280-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48377024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Citrus pectin based complexes for the tetracycline delivery","authors":"Y.V. Chekunkov, S.T. Minzanova, A.V. Khabibullina, D.M. Arkhipova, L.G. Mironova, A.D. Voloshina, A.R. Khamatgalimov, V.A. Milyukov","doi":"10.1016/j.fhfh.2022.100100","DOIUrl":"10.1016/j.fhfh.2022.100100","url":null,"abstract":"<div><p>This research studied the formation of sodium polygalacturonate complexes with the antimicrobial drug \"Tetracycline\". The formation was confirmed by IR, UV and NMR spectroscopy. It was shown that the complexes were formed by electrostatic attraction and hydrogen bonding. The complexes demonstrated a high degree of drug binding to the polysaccharide matrix with the maximum content of tetracycline (6.68 wt %). The study of the antimicrobial activity of obtained compounds against <em>S. aureus, B. cereus, E. coli</em> showed no decrease in the antimicrobial effect compared to source tetracycline. Thus, the future research towards the new design compounds based on studied complexes could make contribution to a new generation of drugs based on pectin biopolymers.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667025922000474/pdfft?md5=cf6948805648618f2ad019a2a3317330&pid=1-s2.0-S2667025922000474-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43234354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Oleogels: Promising alternatives to solid fats for food applications","authors":"Shaziya Manzoor, F.A. Masoodi, Farah Naqash, Rubiya Rashid","doi":"10.1016/j.fhfh.2022.100058","DOIUrl":"10.1016/j.fhfh.2022.100058","url":null,"abstract":"<div><p>Increased concerns over intake of harmful transfats and saturated fats in the diet pose a new challenge to the scientific community, to come up with viable alternatives replacing detrimental fats without affecting organoleptic properties of the food product. Out of various strategies aimed to reduce/replace transfats and saturated fats in foods, oleogels are reported to be an innovative structured fat system used for industrial applications due to their nutritional and environmental benefits. This review will focus on the formulation methods and chemistry of oleogels, along with their recent food applications particularly in bioactive delivery and in other sectors complying with their need. An insight into the mechanism of gelation and various components of oleogels will be deliberated upon. Moreover, modified oleogels with improved technical and functional properties manufactured by use of several emerging technologies like ultrasound will also be reviewed.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667025922000061/pdfft?md5=6a44766b6c8b0540097a28ed72ba80bd&pid=1-s2.0-S2667025922000061-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48679158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahmad El Ghazzaqui Barbosa , Augusto Bene Tomé Constantino , Lívia Pinto Heckert Bastos , Edwin Elard Garcia-Rojas
{"title":"Encapsulation of sacha inchi oil in complex coacervates formed by carboxymethylcellulose and lactoferrin for controlled release of β-carotene","authors":"Ahmad El Ghazzaqui Barbosa , Augusto Bene Tomé Constantino , Lívia Pinto Heckert Bastos , Edwin Elard Garcia-Rojas","doi":"10.1016/j.fhfh.2021.100047","DOIUrl":"10.1016/j.fhfh.2021.100047","url":null,"abstract":"<div><p>This research studied the formation of complex coacervates formed by carboxymethylcellulose (CMC) and lactoferrin (Lf) as wall materials for encapsulation of β-carotene present in sacha inchi oil (SIO). According to zeta-potential and turbidimetric analyses, the optimum conditions for the formation of CMC:Lf complex coacervates were pH 5.0 and a 1:14 ratio. Isothermal titration calorimetry showed that the complexes were formed in two stages: first, the interaction was driven by electrostatic attraction, and second, electrostatic and other interactions (such as hydrogen bonding) or structural conformations were present. The capsules formed with CMC:Lf complex coacervates had a spherical appearance with a well-defined core and were able to encapsulate 97% of SIO. The presence of SIO, CMC, and Lf in the capsules was confirmed by Fourier transform infrared analysis. The <em>in vitro</em> gastrointestinal digestion of capsules showed that 84.31% of β-carotene present in SIO was released in the intestine, with high bioaccessibility (67%). Additionally, Fickian diffusion was the mechanism observed for β-carotene release in the food model. Thus, it is possible to conclude that CMC:Lf complex coacervates are good wall material for encapsulating and protecting β-carotene for food fortification.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266702592100039X/pdfft?md5=21ec8a1d90eb7b401810e7d9999c886e&pid=1-s2.0-S266702592100039X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45672399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sandip Karmakar , Sreejan Manna , Sourav Kabiraj , Sougata Jana
{"title":"Recent progress in alginate-based carriers for ocular targeting of therapeutics","authors":"Sandip Karmakar , Sreejan Manna , Sourav Kabiraj , Sougata Jana","doi":"10.1016/j.fhfh.2022.100071","DOIUrl":"10.1016/j.fhfh.2022.100071","url":null,"abstract":"<div><p>Eye is one of the susceptible organs of the human body which is associated with several internal organs including the brain. Due to the protective mechanisms of body and ocular barrier properties, the area of ocular drug delivery presents a challenge to pharmaceutical researchers. Alginate is a naturally occurring polysaccharide obtained from marine brown seaweeds and bacterial sources. According to the Food and Drug Administration, (FDA) alginate is generally recognized as safe (GRAS). It is widely used as a gelling agent and thickener in the food industries. In the field of drug delivery, alginate is extensively investigated as an excipient. In ocular targeting of therapeutics, sodium alginate offers many advantages including ion sensitive <em>in situ</em> gelation, non-toxic and biodegradable behaviour in combination with mucoadhesive nature of the polymer. The instant gelation ability of alginate allows it to increase the ocular residence time and enhances the ocular drug bioavailability reducing the requirement of frequent administration of drug. The abundant availability and attractive physicochemical properties of alginate has encouraged pharmaceutical scientists to explore newer strategies in ocular drug targeting. In this review, the efficacy of alginate in delivering various therapeutic agents has been discussed.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667025922000188/pdfft?md5=27402f6e434744a196c118844bf395a6&pid=1-s2.0-S2667025922000188-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47466961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Encapsulation of black cumin seed (Nigella sativa) oil by using inverse gelation method","authors":"Recep Palamutoğlu, Cemal Kasnak, Buket Özen","doi":"10.1016/j.fhfh.2022.100089","DOIUrl":"10.1016/j.fhfh.2022.100089","url":null,"abstract":"<div><p>Black cumin seed oil (BCO) is an important oil source in the food industry. Alginate capsules with aqueous cores can be made by reversing the gelation technique with alginate and CaCl<sub>2</sub>. The research aims to investigate the physicochemical properties of the capsules as well as to analyze the storage stability of BCO emulsions (emulgators; polyglycerol polyricinoleate (A) and sorbitan monooleate (B)) coated using the inverse gelation method. The peroxide value in group A increased rapidly on the first day and then declined without any significant difference between the third and sixth days (<em>p</em> > 0.05). The peroxide value of group B increased significantly (<em>p</em> < 0.05) on the first day and then declined like in the other groups. BCO (7.42 ± 0.16) had the lowest p-anisidine value at the beginning with the initial p-anisidine value of encapsulated BCO groups A and B (21.74 ± 0.84 and 11.48 ± 1.31, respectively) having significantly higher (<em>p</em> < 0.05). The reverse gelation technique utilized in this study raised the p-anisidine value while lowering the peroxide value of the seed oil. The shelf life of black seed oil can be increased by using this technique in the industry.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266702592200036X/pdfft?md5=4019947294b54c76b4f5adc34cb5b8e2&pid=1-s2.0-S266702592200036X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43686084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}