Folia microbiologica最新文献_第8页

Preliminary evaluation of a new prototype interferon-gamma release assay for the detection of Mycobacterium tuberculosis-specific T-cell responses in patients with tuberculosis. 用于检测结核患者结核分枝杆菌特异性t细胞反应的新原型干扰素释放试验的初步评估。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-04-01 Epub Date: 2025-03-03 DOI: 10.1007/s12223-025-01252-w

Markéta Ibrahimová, Karolína Doležalová, Luboš Bača, Mariia Sukholytka, Evelin Grage-Griebenow, Dorinja Zapf, Sandra Saschenbrecker, Victor Herbst, Emilia Kopecká, Martina Koziar Vašáková

{"title":"Preliminary evaluation of a new prototype interferon-gamma release assay for the detection of Mycobacterium tuberculosis-specific T-cell responses in patients with tuberculosis.","authors":"Markéta Ibrahimová, Karolína Doležalová, Luboš Bača, Mariia Sukholytka, Evelin Grage-Griebenow, Dorinja Zapf, Sandra Saschenbrecker, Victor Herbst, Emilia Kopecká, Martina Koziar Vašáková","doi":"10.1007/s12223-025-01252-w","DOIUrl":"10.1007/s12223-025-01252-w","url":null,"abstract":"Screening for tuberculosis infections (TBI) using the tuberculin skin test or interferon-gamma release assays (IGRA) is crucial in controlling the global TB burden. This study evaluates the performance of a new IGRA for the detection of T-cell responses against Mycobacterium tuberculosis. Blood samples from 34 adults with tuberculosis disease (TB) and from 30 children with TB, TBI or without TB were analyzed using the prototype Quan-T-Cell TB (EUROIMMUN). The pediatric samples were additionally measured using the established QuantiFERON-TB Gold Plus assay (Qiagen). Clinical performance and inter-assay concordance were analyzed. The prototype Quan-T-Cell TB yielded positivity rates of 88.2% and 100% in adults with TB and children with TBI, respectively, at a specificity of 93.8%. Comparison between the two IGRAs showed positive, negative and overall agreement rates of 100%, 93.8% and 96.3%, respectively, with a kappa score of 0.924 indicating almost perfect agreement. Our study shows promising results of the new prototype Quan-T-Cell TB, as reflected by high concordance with the final diagnosis in adults and children and performance comparable to that of the QuantiFERON IGRA. In individual cases, the data suggest that the prototype Quan-T-Cell TB may be even more consistent with TBI-related clinical findings. Unlike the QuantiFERON assay, the Quan-T-Cell TB has a predefined borderline range, which is advantageous as it may help to differentiate non-specific variation near the cut-off, and fewer sample tubes are required per analysis. The new Quan-T-Cell TB may therefore be a good alternative to the established QuantiFERON IGRA for TBI screening. Further assay optimization is underway, including evaluation studies based on larger patient and control cohorts.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"493-503"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical characteristics and risk factors for the acquisition of an enteric infection by Aeromonas spp. in patients with digestive or nephrological diseases. 消化道或肾脏疾病患者气单胞菌肠道感染的临床特征及危险因素

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-04-01 Epub Date: 2025-01-03 DOI: 10.1007/s12223-024-01239-z

Isabel Lara-Plaza, Emilio Rodrigo-Calabia, Antonio Cuadrado-Lavín, Carlos Ruiz de Alegría-Puig

引用次数: 0

Diversity and composition of rare bacterial community in gut and vermicompost of Eudrilus eugeniae fed with multiple substrates during vermicomposting. 不同基质饲养的eugenius Eudrilus在蚯蚓堆肥过程中肠道和蚯蚓堆肥中稀有细菌群落的多样性和组成。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-03-31 DOI: 10.1007/s12223-025-01257-5

Amirah Alias, Maisarah Ramli, Khoseelaa Vijaya Deventhiran, Md Nurul Islam Siddique, Nurshieren Yahaya, Petr Heděnec

{"title":"Diversity and composition of rare bacterial community in gut and vermicompost of Eudrilus eugeniae fed with multiple substrates during vermicomposting.","authors":"Amirah Alias, Maisarah Ramli, Khoseelaa Vijaya Deventhiran, Md Nurul Islam Siddique, Nurshieren Yahaya, Petr Heděnec","doi":"10.1007/s12223-025-01257-5","DOIUrl":"https://doi.org/10.1007/s12223-025-01257-5","url":null,"abstract":"Vermicomposting has been recognized as a sustainable solution for the managing of organic waste, primarily because of the bacterial communities that drive microbial decomposition. However, while the roles of abundant bacteria in composting processes are well-documented, the contributions of rare bacteria remain underexplored. In this study, we investigated the diversity and composition of abundant and rare bacterial communities in gut of Eudrilus eugeniae and resulting vermicompost, comparing okara as a single substrate compared to a combination of more substrates, such as kitchen waste or okara and kitchen waste. Amplicon sequencing revealed a total of 3085 operational taxonomic units (OTUs), comprising 188 abundant OTUs and 2127 rare OTUs. Significant differences in bacterial community composition were observed between vermicompost and the earthworm gut, particularly in the rare bacterial communities, with distinct abundances of Gemmatimonadota, Desulfobacteria, Myxococcota, Acidobacteria, and Firmicutes. Interestingly, no significant differences were found between treatments in the abundant bacterial communities, suggesting that okara alone can sustain a bacterial community comparable to mixed substrates. However, rare bacterial communities were more sensitive to substrate variation, with okara fostering distinct rare microbial populations in the vermicompost and earthworm gut. Our results indicate okara can support both abundant and rare bacteria, producing compost with similar physico-chemical properties to those derived from mixed substrates. The study highlights the importance of rare bacterial communities in vermicomposting and demonstrates the potential of okara as a valuable resource for sustainable waste management and soil improvement.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential activity of paroxetine alone and associated with oxacillin as an alternative to prevent Staphylococcus aureus biofilm formation in catheters. 单独使用帕罗西汀和联合使用奥西林作为预防导管内金黄色葡萄球菌生物膜形成的替代药物的潜在活性。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-03-28 DOI: 10.1007/s12223-025-01260-w

Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Amanda Dias Barbosa, Lara Elloyse Almeida Moreira, Lívia Gurgel do Amaral Valente Sá, Francisca Bruna Stefany Aires do Nascimento, Cecília Rocha da Silva, João Batista de Andrade Neto, Beatriz Oliveira de Souza, Marina Duarte Pinto Lobo, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior

{"title":"Potential activity of paroxetine alone and associated with oxacillin as an alternative to prevent Staphylococcus aureus biofilm formation in catheters.","authors":"Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Amanda Dias Barbosa, Lara Elloyse Almeida Moreira, Lívia Gurgel do Amaral Valente Sá, Francisca Bruna Stefany Aires do Nascimento, Cecília Rocha da Silva, João Batista de Andrade Neto, Beatriz Oliveira de Souza, Marina Duarte Pinto Lobo, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior","doi":"10.1007/s12223-025-01260-w","DOIUrl":"https://doi.org/10.1007/s12223-025-01260-w","url":null,"abstract":"Biofilm formation, especially in medical devices, is a pertinent factor in the virulence of Staphylococcus aureus, and is known to increase morbidity, mortality, and costs. We evaluated the activity of paroxetine, a selective serotonin reuptake inhibitor, alone and associated with oxacillin, a β-lactam antibacterial, against S. aureus biofilms, as well as verified its potential application as a preventive agent against biofilm formation in catheters. The tests were performed against mature and developing biofilms of methicillin-sensitive and -resistant S. aureus using the thiazolyl blue tetrazolium bromide reduction assay. The prevention of biofilm formation in catheters was investigated by counting colony-forming units, and scanning electron microscopy was also performed. Paroxetine caused a significant reduction in cell viability in biofilms, and when associated with oxacillin, significance was verified. Paroxetine alone and associated with oxacillin showed potential for preventing the formation of S. aureus biofilms in peripheral venous catheters, demonstrated by scanning electron microscopy, reaching inhibition of 94.94% in colony-forming units per mL. Paroxetine demonstrated promising potential against S. aureus biofilms in vitro, indicating the possibility of application as a protective agent against the formation of S. aureus biofilms in catheters.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Native potential probiotics and postbiotics improve the gut-kidney axis by the modulation of autophagy signaling pathway. 天然潜在益生菌和后益生菌通过调节自噬信号通路改善肠肾轴。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-03-12 DOI: 10.1007/s12223-025-01253-9

Fatemeh Haririzadeh Jouriani, Niloofar Rezaie, Fatemeh Ashrafian, Shadi Aghamohammad, Mahdi Rohani

{"title":"Native potential probiotics and postbiotics improve the gut-kidney axis by the modulation of autophagy signaling pathway.","authors":"Fatemeh Haririzadeh Jouriani, Niloofar Rezaie, Fatemeh Ashrafian, Shadi Aghamohammad, Mahdi Rohani","doi":"10.1007/s12223-025-01253-9","DOIUrl":"https://doi.org/10.1007/s12223-025-01253-9","url":null,"abstract":"The gut-kidney axis is the bidirectional relationship between the gut microbiota and the kidney function. Chronic inflammatory responses can impair kidney function and probiotics and postbiotics agents can have positive effects on gut health and kidney function by modulating inflammation through affecting autophagy signaling pathway. The aim of the current study was to evaluate the properties of our probiotic and postbiotics to improve kidney health by focusing the autophagy signaling pathway. The probiotic and postbiotics of four Lactobacillus and two Bifidobacterium strains were selected. Dextran sulfate sodium induced colitis in mice, and probiotics and postbiotics treatments were accomplished in animal experiment. A qPCR assay was performed to assess the gene expression involved in the autophagy process in the kidney. In contrast to the dextran sulfate sodium group, both the probiotic and postbiotics cocktails exhibited the capacity to inhibit colitis-associated indicators. Of note, the postbiotics cocktails demonstrated a greater efficacy in preventing colitis-related indicators and also it could display a more pronounced effect in upregulating autophagy-related genes. Our native potential probiotics and postbiotics can be able to reduce gut inflammation and cope with kidney inflammation by triggering autophagy signaling pathway through the considerable impact on gut-organ axis. There is an encouraging concept about the anti-inflammatory effects of our probiotics and postbiotics cocktails with least side effects as a supplementary treatment not only in the gut, but also in the other organs particularly kidneys.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of plant growth-promoting endophytic fungi from Aegle marmelos Corr. and their role in growth enhancement and yield in rice. 柑橘属植物促生长内生真菌的鉴定及其对水稻生长和产量的促进作用。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-03-04 DOI: 10.1007/s12223-025-01254-8

Swetha Parakkulathil Ramachandran, Vivek Padmanabhan Jayanthikumari, Fasna Kodavanthodi, Resmi Mohankumar Saraladevi

{"title":"Characterization of plant growth-promoting endophytic fungi from Aegle marmelos Corr. and their role in growth enhancement and yield in rice.","authors":"Swetha Parakkulathil Ramachandran, Vivek Padmanabhan Jayanthikumari, Fasna Kodavanthodi, Resmi Mohankumar Saraladevi","doi":"10.1007/s12223-025-01254-8","DOIUrl":"https://doi.org/10.1007/s12223-025-01254-8","url":null,"abstract":"Plant growth-promoting endophytes (PGPE) are microorganisms which reside in plant tissues and are beneficial to the host in plant growth promotion and pathogen resistance. They are the eco-friendly and sustainable alternative to chemical fertilizers and pesticides. This study aimed to analyze the plant growth-promoting properties of the five endophytic fungal strains from the medicinal plant Aegle marmelos Corr. and evaluate their effects on Oryza sativa plants. Firstly, endophytes were isolated from the different parts of A. marmelos and identified by ITS sequencing. Phosphate solubilization ability was checked in Pikovskaya's agar medium, IAA secretion was measured by the Salkowski colourimetric method, and ACC deaminase activity was checked by Penrose's method. Four endophytic fungal strains with promising PGP activities were inoculated into rice seeds to check their growth promotion in rice. The strain Purpureocillium lilacinum (AMR2) enhanced the seed vigour of rice seeds and demonstrated excellent root colonization ability. Periconia byssoides (AML2) and Medicopsis romeroi (AMS3) were the most effective plant growth-promoting agents, leading to both crop yield improvement and enhanced plant morphological growth due to their great ability to solubilize inorganic phosphate, ACC deaminase activity and production of IAA and Gibberellin A3 (GA3). These endophytic strains could serve as microbial inoculants to enhance crop production, offering an eco-friendly alternative.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection and phylogeny of Hepatozoon ophisauri and Toxoplasma gondii in wild lizards from Khyber Pakhtunkhwa, Pakistan. 巴基斯坦开伯尔-普赫图赫瓦省野生蜥蜴中蛇肝虫和刚地弓形虫的分子检测和系统发育。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-22 DOI: 10.1007/s12223-025-01250-y

Sidra Ghafar, Asmat Ullah Khan, Mustansar Khalid, Sami Ul Haq, Saeed Ullah, Umaima Basit, Hira Muqaddas, Ibrahim Abbas Mohammed, Ayat Ammar Mahdi, Adil Khan, Mourad Ben Said, Furhan Iqbal

{"title":"Molecular detection and phylogeny of Hepatozoon ophisauri and Toxoplasma gondii in wild lizards from Khyber Pakhtunkhwa, Pakistan.","authors":"Sidra Ghafar, Asmat Ullah Khan, Mustansar Khalid, Sami Ul Haq, Saeed Ullah, Umaima Basit, Hira Muqaddas, Ibrahim Abbas Mohammed, Ayat Ammar Mahdi, Adil Khan, Mourad Ben Said, Furhan Iqbal","doi":"10.1007/s12223-025-01250-y","DOIUrl":"https://doi.org/10.1007/s12223-025-01250-y","url":null,"abstract":"Despite Pakistan's rich reptilian diversity, wild lizards have largely been unexplored for vector-borne parasites. This study reports the prevalence and phylogenetic assessment of Hepatozoon ophisauri and Toxoplasma gondii DNA in the blood samples of 101 wild lizards from Khyber Pakhtunkhwa, Pakistan, captured at altitudes of 1200 to 2250 m above sea level between March 2022 and June 2023. PCR-based molecular analysis identified H. ophisauri DNA in three (3%) lizards of all Laudakia tuberculata. Additionally, 45 (44.5%) lizards tested positive for T. gondii DNA, including L. agrorensis, L. pakistanica, L. tuberculata, and Abblepharus pannonicus. Sequence analysis and BLAST confirmed the presence of H. ophisauri and T. gondii. Phylogenetic analysis showed genetic diversity among the H. ophisauri and T. gondii nucleotide sequences, clustering with reference sequences from reptiles, birds, and ticks. Toxoplasma gondii prevalence varied among species, with the highest rates in L. agrorensis and A. pannonicus (67%), followed by L. pakistanica (45%) and L. tuberculata (43%). This study is the first from Pakistan to report high T. gondii prevalence and low H. ophisauri prevalence in wild lizards. Similar and larger-scale studies in unexplored regions of Pakistan are needed to enhance understanding of these pathogens' genetic diversity and host-parasite interactions.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metarhizium anisopliae engineering mediated by a CRISPR/Cas9 recyclable system. CRISPR/Cas9可循环系统介导的绿僵菌工程。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-21 DOI: 10.1007/s12223-025-01249-5

Maria Letícia de Siqueira Virgílio, Eliane Dias Quintela, Liriel Helen Rodrigues Maciel, Gabriela Souza Silva Goulart, José Francisco Arruda E Silva, Marcio Vinicius de Carvalho Barros Cortes

{"title":"Metarhizium anisopliae engineering mediated by a CRISPR/Cas9 recyclable system.","authors":"Maria Letícia de Siqueira Virgílio, Eliane Dias Quintela, Liriel Helen Rodrigues Maciel, Gabriela Souza Silva Goulart, José Francisco Arruda E Silva, Marcio Vinicius de Carvalho Barros Cortes","doi":"10.1007/s12223-025-01249-5","DOIUrl":"https://doi.org/10.1007/s12223-025-01249-5","url":null,"abstract":"The advent of CRISPR/Cas technology has revolutionized genome editing, offering simplicity, precision, and cost-effectiveness. While its application in biological control fungi has been limited, including the cosmopolitan fungus Metarhizium anisopliae, recent advancements show promise. However, integrating cas9 and selection-marker genes into fungal genomes poses challenges, including reduced efficiency, toxicity, and off-target effects. Besides, marker-free genetic engineering through a CRISPR recyclable system presents a viable solution, enabling efficient mutant generation without compromising fitness and virulence. This study pioneers the construction of marker-free strains of M. anisopliae using a CRISPR/Cas9 recyclable system. Precise deletion of albA and ku70, alongside gfp cassette insertion, confirms the system efficiency. This innovative approach holds significant potential for facilitating in-depth molecular studies, understanding their ecological roles in agricultural systems, and enhancing biocontrol efficacy against insect pests through genetic improvement.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced recombinant lipase production in Pseudomonas aeruginosa SDK-6: medium optimization using OFAT and RSM with purification and stability studies. 铜绿假单胞菌SDK-6重组脂肪酶的增强：利用OFAT和RSM优化培养基，并进行纯化和稳定性研究。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-17 DOI: 10.1007/s12223-025-01248-6

Damanjeet Kaur, Saurabh Gupta

{"title":"Enhanced recombinant lipase production in Pseudomonas aeruginosa SDK-6: medium optimization using OFAT and RSM with purification and stability studies.","authors":"Damanjeet Kaur, Saurabh Gupta","doi":"10.1007/s12223-025-01248-6","DOIUrl":"https://doi.org/10.1007/s12223-025-01248-6","url":null,"abstract":"Lipases are among the most significant biocatalysts that constitute the third most important group of enzymes due to their vast range of applications. The present research represents the first attempt to optimize the growth medium constituents to increase the production of recombinant lipase in Pseudomonas aeruginosa SDK-6. One factor at a time (OFAT) revealed castor oil, yeast extract, and ammonium nitrate as the most significant medium components affecting the recombinant lipase production. Further optimization via response surface methodology (RSM) resulted in lipase production of 115.50 U/mL with 0.5% (v/v) castor oil, 0.2% (w/v) yeast extract, and 0.1% (w/v) ammonium nitrate at pH 7. Statistical validation of the observed value via ANOVA revealed an F value of 117.02 at p < 0.01, with an R2 of 0.9909. An overall 3.58-fold lipase production was achieved after optimization via OFAT and RSM. The purified lipase exhibited a specific activity of 102.73 U/mg, and the molecular mass was deduced to ~ 19.5 kDa via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimum temperature and pH for the recombinant lipase activity were 40 °C and 10, respectively. The enzyme retained most of its initial activity up to 32 h when incubated at an elevated temperature of 40 °C. The purified enzyme also exhibited stability over alkaline pH, with remarkable stability at pH 12. The enzyme activity was enhanced in the presence of CaCl2, MgCl2, FeCl2, NaCl, methanol, dichloromethane, and Triton X-100. The enzyme also retained most of its initial activity in the presence of all other screened organic solvents.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional modulation of Porphyromonas gingivalis biofilms on titanium-copper implant surfaces. 钛铜种植体表面牙龈卟啉单胞菌生物膜的转录调控。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-15 DOI: 10.1007/s12223-025-01246-8

Vinoj Gopalakrishnan, Maria Infant Majula Shifani Mahendran, Vaijayanthi Saravanan, Dhamodharan Ramaswamy

{"title":"Transcriptional modulation of Porphyromonas gingivalis biofilms on titanium-copper implant surfaces.","authors":"Vinoj Gopalakrishnan, Maria Infant Majula Shifani Mahendran, Vaijayanthi Saravanan, Dhamodharan Ramaswamy","doi":"10.1007/s12223-025-01246-8","DOIUrl":"https://doi.org/10.1007/s12223-025-01246-8","url":null,"abstract":"The healthcare sector is currently concerned about infections caused by Porphyromonas gingivalis biofilms due to their high frequency and incidence, particularly in patients with implanted medical devices. This study investigated biofilm formation and biofilm-related gene expression in P. gingivalis on titanium-copper discs and polycarbonate discs. P. gingivalis highly expressed biofilm-related genes were examined using quantitative real-time PCR during biofilm formation on the Ti-Cu surface. SEM analysis revealed various cellular components around the aggregated cells at various stages of biofilm formation. The Ti-Cu surface was colonized by P. gingivalis, as evidenced by biofilm formation levels that varied from ~ 103-104 CFU/cm2 after 2 days of incubation to ~ 105-107 CFU/cm2 after 7 days. Real-time expression analysis showed a significant increase in the expression of signaling molecules on Ti-Cu discs. Furthermore, genes linked to virulence (rgpA, rgpB, and Kgp, fimC, PorK, and PorP) and adhesion (mfa1, fimD, fimA, RpoN, rgpA, rgpBiKgp) demonstrate transcriptional alterations in signaling pathways impacting P. gingivalis biofilm on Ti-Cu surfaces. Scanning electron microscopy (SEM) and confocal microscopy correlated the results of the structural analysis with the expression from the qPCR data. This study adds significant value by advancing the understanding of biofilm formation on Ti-Cu implants.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0