Fuxiang Li, Huafeng Gao, Wenhua Zhao, Jianling Song
{"title":"从山羊呼吸道疾病中分离的鼻杆菌。","authors":"Fuxiang Li, Huafeng Gao, Wenhua Zhao, Jianling Song","doi":"10.1007/s12223-025-01279-z","DOIUrl":null,"url":null,"abstract":"<p><p>A Gram-stain-negative, aerobic and rod-shaped bacteria, designated ZY210820<sup>T</sup>, was isolated from the nasal cavity of a goat with respiratory disease in Yunnan Province, PR China. The strain grew at pH 6.0-9.0 (optimum pH 7.0), at 24-39 °C (optimum 37 °C) and with 0.5-2.0% (w/v) NaCl (optimum 1.0% NaCl). Phylogenetic analysis of 16S rRNA gene sequences showed that the strain formed a separated branch within the family Moraxellaceae with the highest similarity of 93.1% to Acinetobacter kanungonis JCM 34131<sup>T</sup>. Phylogenomic analysis of 327 single-copy protein sequences revealed that the strain belonged to the family Moraxellaceae and constituted a separated branch. The genomic G + C content was 35.5%. The highest orthologous average nucleotide identity (OrthoANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) values between the strain and the type strains in the family Moraxellaceae were 72.5% (Acinetobacter calcoaceticus DSM 30006<sup>T</sup>), 37.0% (Fluviicoccus keumensis JCM 19370<sup>T</sup>), and 64.0% (Acinetobacter kanungonis JCM 34131<sup>T</sup>), respectively. The predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, cardiolipin, monolysocardiolipin, and diacylglycerol. The strain contained C<sub>12:0</sub>, C<sub>16:0</sub>, summed feature 3 (C<sub>16:1</sub>ω7c and/ or C<sub>16:1</sub>ω6c), C<sub>17:0</sub>, and C<sub>15:1</sub>ω6c as the major fatty acid (> 5%) and CoQ-9 as the major respiratory quinone. The results of the polyphasic analysis revealed that strain ZY210820<sup>T</sup> represents a novel species of a new genus in the family Moraxellaceae, and the name Nasibacterium caprae gen. nov., sp. nov. is proposed. The type strain is ZY210820<sup>T</sup> (= CCTCC AB 2021475<sup>T</sup> = NBRC 115474<sup>T</sup>).</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Nasibacterium caprae gen. nov., sp. nov., isolated from a goat with respiratory disease.\",\"authors\":\"Fuxiang Li, Huafeng Gao, Wenhua Zhao, Jianling Song\",\"doi\":\"10.1007/s12223-025-01279-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A Gram-stain-negative, aerobic and rod-shaped bacteria, designated ZY210820<sup>T</sup>, was isolated from the nasal cavity of a goat with respiratory disease in Yunnan Province, PR China. The strain grew at pH 6.0-9.0 (optimum pH 7.0), at 24-39 °C (optimum 37 °C) and with 0.5-2.0% (w/v) NaCl (optimum 1.0% NaCl). Phylogenetic analysis of 16S rRNA gene sequences showed that the strain formed a separated branch within the family Moraxellaceae with the highest similarity of 93.1% to Acinetobacter kanungonis JCM 34131<sup>T</sup>. Phylogenomic analysis of 327 single-copy protein sequences revealed that the strain belonged to the family Moraxellaceae and constituted a separated branch. The genomic G + C content was 35.5%. The highest orthologous average nucleotide identity (OrthoANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) values between the strain and the type strains in the family Moraxellaceae were 72.5% (Acinetobacter calcoaceticus DSM 30006<sup>T</sup>), 37.0% (Fluviicoccus keumensis JCM 19370<sup>T</sup>), and 64.0% (Acinetobacter kanungonis JCM 34131<sup>T</sup>), respectively. The predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, cardiolipin, monolysocardiolipin, and diacylglycerol. The strain contained C<sub>12:0</sub>, C<sub>16:0</sub>, summed feature 3 (C<sub>16:1</sub>ω7c and/ or C<sub>16:1</sub>ω6c), C<sub>17:0</sub>, and C<sub>15:1</sub>ω6c as the major fatty acid (> 5%) and CoQ-9 as the major respiratory quinone. The results of the polyphasic analysis revealed that strain ZY210820<sup>T</sup> represents a novel species of a new genus in the family Moraxellaceae, and the name Nasibacterium caprae gen. nov., sp. nov. is proposed. The type strain is ZY210820<sup>T</sup> (= CCTCC AB 2021475<sup>T</sup> = NBRC 115474<sup>T</sup>).</p>\",\"PeriodicalId\":12346,\"journal\":{\"name\":\"Folia microbiologica\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-06-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Folia microbiologica\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s12223-025-01279-z\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Folia microbiologica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12223-025-01279-z","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Nasibacterium caprae gen. nov., sp. nov., isolated from a goat with respiratory disease.
A Gram-stain-negative, aerobic and rod-shaped bacteria, designated ZY210820T, was isolated from the nasal cavity of a goat with respiratory disease in Yunnan Province, PR China. The strain grew at pH 6.0-9.0 (optimum pH 7.0), at 24-39 °C (optimum 37 °C) and with 0.5-2.0% (w/v) NaCl (optimum 1.0% NaCl). Phylogenetic analysis of 16S rRNA gene sequences showed that the strain formed a separated branch within the family Moraxellaceae with the highest similarity of 93.1% to Acinetobacter kanungonis JCM 34131T. Phylogenomic analysis of 327 single-copy protein sequences revealed that the strain belonged to the family Moraxellaceae and constituted a separated branch. The genomic G + C content was 35.5%. The highest orthologous average nucleotide identity (OrthoANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) values between the strain and the type strains in the family Moraxellaceae were 72.5% (Acinetobacter calcoaceticus DSM 30006T), 37.0% (Fluviicoccus keumensis JCM 19370T), and 64.0% (Acinetobacter kanungonis JCM 34131T), respectively. The predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, cardiolipin, monolysocardiolipin, and diacylglycerol. The strain contained C12:0, C16:0, summed feature 3 (C16:1ω7c and/ or C16:1ω6c), C17:0, and C15:1ω6c as the major fatty acid (> 5%) and CoQ-9 as the major respiratory quinone. The results of the polyphasic analysis revealed that strain ZY210820T represents a novel species of a new genus in the family Moraxellaceae, and the name Nasibacterium caprae gen. nov., sp. nov. is proposed. The type strain is ZY210820T (= CCTCC AB 2021475T = NBRC 115474T).
期刊介绍：
Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
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