Folia microbiologica最新文献_第9页

Goan mangrove yeast: a source of therapeutic enzyme L-asparaginase. 果阿红树林酵母：治疗酶l -天冬酰胺酶的来源。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-13 DOI: 10.1007/s12223-025-01247-7

Aabha Marathe, Lakshangy Charya

{"title":"Goan mangrove yeast: a source of therapeutic enzyme L-asparaginase.","authors":"Aabha Marathe, Lakshangy Charya","doi":"10.1007/s12223-025-01247-7","DOIUrl":"https://doi.org/10.1007/s12223-025-01247-7","url":null,"abstract":"Mangrove forests support robust microorganisms due to their changing temperature and salinity conditions. Tropical yeasts isolated from such habitats have the potential to produce clinically significant enzymes like L-asparaginase. L-asparaginase converts L-asparagine to L-aspartate and ammonia. In this study, 14 tropical yeasts were isolated from mangrove sediments collected from Madkai, Goa, India. From these, on screening with two different indicator dyes phenol red and bromothymol blue, five isolates were positive for L-asparaginase enzyme as indicated by rapid plate assay technique. Isolate having highest enzyme activity was identified as belonging to genus Cyberlindnera. The study of effect of physical parameters on enzyme production revealed optimal incubation time of 48 h, pH of medium 7.0 and incubation temperature of 28 °C. Evaluation of carbon and nitrogen sources indicated L-asparagine as sole nitrogen source and glucose as carbon source achieved maximum enzyme production. L-Asparaginase activity of isolate GULAMMS8 under optimal medium composition and parameter conditions that are glucose as carbon source with L-asparagine as sole nitrogen source and pH of 7.0, an incubation temperature of 28 °C showed a two-fold increase, while the incubation period was halved. This work is a primary study on L-asparaginase producing tropical mangrove yeast belonging to genus Cyberlindnera aiming to understand the influence of physical factors and nutrient sources on enzyme production.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotic characteristics and survival of a multi-strain lactic acid bacteria consortium in simulated gut model. 模拟肠道模型中多菌株乳酸菌群的益生菌特性和存活

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-09 DOI: 10.1007/s12223-025-01244-w

Muhammad Nadeem Khan, Saeeda Bashir, Afifa Afzal, Naghmana Andleeb, Lukasz Krych, Dennis Sandris Nielsen, Muhammad Imran

{"title":"Probiotic characteristics and survival of a multi-strain lactic acid bacteria consortium in simulated gut model.","authors":"Muhammad Nadeem Khan, Saeeda Bashir, Afifa Afzal, Naghmana Andleeb, Lukasz Krych, Dennis Sandris Nielsen, Muhammad Imran","doi":"10.1007/s12223-025-01244-w","DOIUrl":"https://doi.org/10.1007/s12223-025-01244-w","url":null,"abstract":"Dahi, a traditional yet underexplored fermented milk product from Pakistan, harbors diverse lactic acid bacteria (LAB) that have potential as probiotics. These bacteria could be used for therapeutic purposes, beneficial modulation of gut microbiota, and in the formulation of functional foods and feeds. This study aimed to isolate and characterize probiotic LAB from dahi, assess their survival in simulated gastrointestinal conditions, and evaluate their safety and probiotic potential, both phenotypically and genotypically. A total of 143 isolates from 37 samples were evaluated for probiotic traits, including acid and bile tolerance, antibacterial activity, cholesterol-lowering capacity, and antioxidant activity. The strains were also tested for antibiotic sensitivity and safety through in vitro tests and genomic analysis. A multi-strain probiotic consortium was developed and tested for enhanced functionality. Out of 143 isolates, 62 LAB strains were identified. These strains demonstrated significant survival under acidic (pH 2) and bile conditions. Antibacterial activity against pathogens ranged from 51 to 88%. The strains exhibited high cholesterol removal (up to 98%) and antioxidant activity (up to 76%). Genomic analysis revealed the presence of key probiotic-related genes, including those for acid resistance, bile salt hydrolase, and adhesion. All strains were sensitive to EFSA-recommended antibiotics and exhibited no hemolytic or DNase activity, confirming their safety. The multi-strain consortium showed superior probiotic potential and survival in simulated gastrointestinal conditions. LAB strains isolated from dahi possess strong probiotic potential, confirmed through in vitro and genomic safety assessments. The multi-strain consortium holds promise for applications.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing multi-season wheat yield through plant growth-promoting rhizobacteria using consortium and individual isolate applications. 促进植物生长的根瘤菌联合和单株分离应用提高小麦多季产量。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-05 DOI: 10.1007/s12223-025-01245-9

Gerhardus Breedt, Lise Korsten, Jarishma Keriuscia Gokul

{"title":"Enhancing multi-season wheat yield through plant growth-promoting rhizobacteria using consortium and individual isolate applications.","authors":"Gerhardus Breedt, Lise Korsten, Jarishma Keriuscia Gokul","doi":"10.1007/s12223-025-01245-9","DOIUrl":"https://doi.org/10.1007/s12223-025-01245-9","url":null,"abstract":"In recent decades, there has been a growing interest in harnessing plant growth-promoting rhizobacteria (PGPR) as a possible mechanism to mitigate the environmental impact of conventional agricultural practices and promote sustainable agricultural production. This study investigated the transferability of promising PGPR research from maize to another Poaceae cereal crop, wheat. This multi-seasonal study evaluated the wheat grain yield effect of Lysinibacillus sphaericus (T19), Paenibacillus alvei (T29) when applied i. individually, ii. as a consortium with Bacillus safensis (S7), and iii. at a 75% reduced fertilizer rate. Whole genome sequencing allowed annotation of genes linked to plant growth promotion, providing potential genomic explanations for the observed in-field findings. Application of the consortium compared to a commercial PGPR showed significantly increased wheat yield by 30.71%, and 25.03%, respectively, in season one, and 63.92% and 58.45%, respectively, under reduced fertilizer rates in season two. Individual application of T19 and T29 showed varying results, with T19 increasing wheat yield by 9.33% and 16.22% during seasons three and four but a substantial reduction (33.39%) during season five. T29 exhibited yield increases during season three (9.31%) and five (5.61%) but led to a significant reduction (21.15%) in season four. Genomic analysis unveiled a spectrum of plant growth-promoting genes including those associated with ammonification, phosphate solubilization, ethylene, siderophore, catalase, and superoxide dismutase production. These findings offer valuable insights into the mechanisms behind observed field results, with potential implications for advancing sustainable agriculture and crop productivity in evolving agricultural landscapes.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nano-coating with silicon dioxide to reduce the occurrence of bacterial contamination in a pig abattoir drinking system. 纳米二氧化硅涂层减少猪屠宰场饮水系统中细菌污染的发生。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-04 DOI: 10.1007/s12223-025-01243-x

Celine Buder, Nina Langkabel, Alina Kirse, Mirjam Kalusa, Simone A Fietz, Diana Meemken

{"title":"Nano-coating with silicon dioxide to reduce the occurrence of bacterial contamination in a pig abattoir drinking system.","authors":"Celine Buder, Nina Langkabel, Alina Kirse, Mirjam Kalusa, Simone A Fietz, Diana Meemken","doi":"10.1007/s12223-025-01243-x","DOIUrl":"https://doi.org/10.1007/s12223-025-01243-x","url":null,"abstract":"A recently discovered source for infection of slaughter pigs, and thus entry for bacteria into the food chain, is the installed drinking equipment in lairage pens of pig abattoirs. To mitigate this, nano-coating of stainless steel, currently used in human medicine fields as well as in other parts of the food chain, appears as promising technology. In this study, silicon dioxide nano-coating was applied to six drinkers and installed for one and three months in a lairage of a pig abattoir, while results were compared with those of drinkers that had not been nano-coated. Laboratory examination of eight sample types related to the drinkers was conducted for total aerobic plate count, Enterobacteriaceae count, Pseudomonas spp. count, Salmonella presence, pathogenic Yersinia enterocolitica presence, Listeria monocytogenes presence and methicillin-resistant Staphylococcus aureus presence. The nipple drinker, which the pigs take into their mouth for drinking, was then examined using scanning electron microscopy and elemental analysis. The nano-coating did not produce statistically significant reductions in the loads or presence of these bacteria compared to the same but uncoated drinking equipment used under the same conditions. Further studies should focus on the implementation of combined methods, such as nano-coating and sanitary treatment, as well as modifications to the coating itself, to produce meaningful reductions of the bacterial loads on/in abattoir lairage drinking equipment.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphonium chloride-based deep eutectic solvents inhibit pathogenic Acanthamoeba castellanii belonging to the T4 genotype. 基于氯化鏻的深共晶溶剂对属于 T4 基因型的致病性卡斯特阿米巴有抑制作用。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-01 Epub Date: 2024-06-13 DOI: 10.1007/s12223-024-01180-1

Noor Akbar, Amir Sada Khan, Ruqaiyyah Siddiqui, Taleb Hassan Ibrahim, Mustafa I Khamis, Bader S Alawfi, Bassam M Al-Ahmadi, Naveed Ahmed Khan

{"title":"Phosphonium chloride-based deep eutectic solvents inhibit pathogenic Acanthamoeba castellanii belonging to the T4 genotype.","authors":"Noor Akbar, Amir Sada Khan, Ruqaiyyah Siddiqui, Taleb Hassan Ibrahim, Mustafa I Khamis, Bader S Alawfi, Bassam M Al-Ahmadi, Naveed Ahmed Khan","doi":"10.1007/s12223-024-01180-1","DOIUrl":"10.1007/s12223-024-01180-1","url":null,"abstract":"Herein, we investigated the anti-amoebic activity of phosphonium-chloride-based deep eutectic solvents against pathogenic Acanthamoeba castellanii of the T4 genotype. Deep eutectic solvents are ionic fluids composed of two or three substances, capable of self-association to form a eutectic mixture with a melting point lower than each substance. In this study, three distinct hydrophobic deep eutectic solvents were formulated, employing trihexyltetradecylphosphonium chloride as the hydrogen bond acceptor and aspirin, dodecanoic acid, and 4-tert-butylbenzoic acid as the hydrogen bond donors. Subsequently, all three deep eutectic solvents, denoted as DES1, DES2, DES3 formulations, underwent investigations comprising amoebicidal, adhesion, excystation, cytotoxicity, and cytopathogenicity assays. The findings revealed that DES2 was the most potent anti-amoebic agent, with a 94% elimination rate against the amoebae within 24 h at 30 °C. Adhesion assays revealed that deep eutectic solvents hindered amoebae adhesion to human brain endothelial cells, with DES2 exhibiting 88% reduction of adhesion. Notably, DES3 exhibited remarkable anti-excystation properties, preventing 94% of cysts from reverting to trophozoites. In cytopathogenicity experiments, deep eutectic solvent formulations and dodecanoic acid alone reduced amoebae-induced human brain endothelial cell death, with DES2 showing the highest effects. Lactate dehydrogenase assays revealed the minimal cytotoxicity of the tested deep eutectic solvents, with the exception of trihexyltetradecylphosphonium chloride, which exhibited 35% endothelial cell damage. These findings underscore the potential of specific deep eutectic solvents in combating pathogenic Acanthamoeba, presenting promising avenues for further research and development against free-living amoebae.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"101-113"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of gut microbiome in developing necrotizing enterocolitis. 肠道微生物组在发生坏死性小肠结肠炎中的作用。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-01 Epub Date: 2024-11-17 DOI: 10.1007/s12223-024-01217-5

Cevher Karaca, Hatice Aysun Mercimek Takcı

{"title":"Role of gut microbiome in developing necrotizing enterocolitis.","authors":"Cevher Karaca, Hatice Aysun Mercimek Takcı","doi":"10.1007/s12223-024-01217-5","DOIUrl":"10.1007/s12223-024-01217-5","url":null,"abstract":"Necrotizing enterocolitis (NEC) is one of the most devastating intestinal diseases observed in preterm in the first days of life. Researchers have recently focused on potential predictive biomarkers for early and concomitant diagnoses. Thus, we inquired about the linkage of intestinal dysbiosis, one of the most important factors in NEC development to the gut microbiota. In this study, the systematic differences in the bacterial composition between neonates affected by NEC and healthy newborns were highlighted by metagenomic analysis. The next-generation sequencing of the V3-V4 variable region of the 16S rRNA gene and gene-specific qPCR analyzed the untargeted gut microbiota. Total bacteria, total and fecal coliform loads in stool samples with NEC were higher than control. OTU-level relative abundances of NEC infant was characterized by Firmicutes and Bacteroidetes at phylum levels. At the genus level, NEC stool was identified by the lack of Klebsiella and the presence of Roseburia, Blautia, and Parasutterella. Finally, Clostridium fessum was the predominant species of Clostridium genus in disease and healthy specimens at the species level, whereas Clostridium jeddahitimonense was at NEC diagnosis. Despite a strong relationship between pathophysiology and characterization of gut microbiota at a clinical diagnosis of NEC, our results emphasize the broad difficulty in identifying potential biomarkers.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"197-204"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial activity of gallic acid and methyl gallate against emerging non-fermenting bacilli. 没食子酸和没食子酸甲酯对新出现的非发酵菌的抗菌活性。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-01 Epub Date: 2024-06-21 DOI: 10.1007/s12223-024-01182-z

Orlando Flores-Maldonado, Jorge Dávila-Aviña, Gloria M González, Miguel A Becerril-García, Ana L Ríos-López

{"title":"Antibacterial activity of gallic acid and methyl gallate against emerging non-fermenting bacilli.","authors":"Orlando Flores-Maldonado, Jorge Dávila-Aviña, Gloria M González, Miguel A Becerril-García, Ana L Ríos-López","doi":"10.1007/s12223-024-01182-z","DOIUrl":"10.1007/s12223-024-01182-z","url":null,"abstract":"Stenotrophomonas maltophilia, Achromobacter xylosoxidans, and Burkholderia cenocepacia are considered emerging pathogens classified as a public health problem due to extensive antimicrobial resistance. Therefore, the discovery of new therapeutic strategies has become crucial. This study aimed to evaluate the antimicrobial activity of gallic acid and methyl gallate against non-fermenting bacteria. The study included five clinical isolates of Stenotrophomonas maltophilia, Achromobacter xylosoxidans, and Burkholderia cenocepacia. The minimum inhibitory concentrations of gallic acid and methyl gallate were determined by the broth microdilution method. Growth curves, metabolic activity, and biofilm formation of each bacterial strain in the presence or absence of phenolic compounds were performed. Finally, the therapeutic efficacy of the compounds was evaluated using an in vivo model. Gallic acid and methyl gallate showed antibacterial activity against bacterial strains in a concentration range of 64 to 256 µg/mL, both compounds reduced bacterial growth and metabolic activity of the strains, even at subinhibitory concentrations. Only, methyl gallate exhibited activity to inhibit the formation of bacterial biofilms. Moreover, gallic acid and methyl gallate increased larval survival by up to 60% compared to 30% survival of untreated larvae in a bacterial infection model in Galleria mellonella. Our results highlight the potential of gallic acid and methyl gallate as therapeutic alternatives for infections by emerging non-fermentative bacteria.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"127-135"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment. 通过流式细胞仪和荧光活力评估来评价蓝藻的应激恢复能力。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-01 Epub Date: 2024-11-06 DOI: 10.1007/s12223-024-01212-w

Zuzana Kroupová, Eva Slaninová, Kateřina Mrázová, Vladislav Krzyžánek, Kamila Hrubanová, Ines Fritz, Stanislav Obruča

{"title":"Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment.","authors":"Zuzana Kroupová, Eva Slaninová, Kateřina Mrázová, Vladislav Krzyžánek, Kamila Hrubanová, Ines Fritz, Stanislav Obruča","doi":"10.1007/s12223-024-01212-w","DOIUrl":"10.1007/s12223-024-01212-w","url":null,"abstract":"Cyanobacteria are prokaryotic organisms characterised by their complex structures and a wide range of pigments. With their ability to fix CO2, cyanobacteria are interesting for white biotechnology as cell factories to produce various high-value metabolites such as polyhydroxyalkanoates, pigments, or proteins. White biotechnology is the industrial production and processing of chemicals, materials, and energy using microorganisms. It is known that exposing cyanobacteria to low levels of stressors can induce the production of secondary metabolites. Understanding of this phenomenon, known as hormesis, can involve the strategic application of controlled stressors to enhance the production of specific metabolites. Consequently, precise measurement of cyanobacterial viability becomes crucial for process control. However, there is no established reliable and quick viability assay protocol for cyanobacteria since the task is challenging due to strong interferences of autofluorescence signals of intercellular pigments and fluorescent viability probes when flow cytometry is used. We performed the screening of selected fluorescent viability probes used frequently in bacteria viability assays. The results of our investigation demonstrated the efficacy and reliability of three widely utilised types of viability probes for the assessment of the viability of Synechocystis strains. The developed technique can be possibly utilised for the evaluation of the importance of polyhydroxyalkanoates for cyanobacterial cultures with respect to selected stressor-repeated freezing and thawing. The results indicated that the presence of polyhydroxyalkanoate granules in cyanobacterial cells could hypothetically contribute to the survival of repeated freezing and thawing.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"205-223"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic analysis of a halophilic bacterium Nesterenkonia sp. CL21 with ability to produce a diverse group of lignocellulolytic enzymes. 对具有生产多种木质纤维素分解酶能力的嗜卤细菌 Nesterenkonia sp.

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-01 Epub Date: 2024-06-06 DOI: 10.1007/s12223-024-01178-9

Hongxuan An, Xin Huey Ching, Wai Jun Cheah, Wei Lun Lim, Kah Yaw Ee, Chun Shiong Chong, Ming Quan Lam

{"title":"Genomic analysis of a halophilic bacterium Nesterenkonia sp. CL21 with ability to produce a diverse group of lignocellulolytic enzymes.","authors":"Hongxuan An, Xin Huey Ching, Wai Jun Cheah, Wei Lun Lim, Kah Yaw Ee, Chun Shiong Chong, Ming Quan Lam","doi":"10.1007/s12223-024-01178-9","DOIUrl":"10.1007/s12223-024-01178-9","url":null,"abstract":"Halophilic bacteria are extremophiles that thrive in saline environment. Their ability to withstand such harsh conditions makes them an ideal choice for industrial applications such as lignocellulosic biomass degradation. In this study, a halophilic bacterium with the ability to produce extracellular cellulases and hemicellulases, designated as Nesterenkonia sp. CL21, was isolated from mangrove sediment in Tanjung Piai National Park, Malaysia. Thus far, studies on lignocellulolytic enzymes concerning bacterial species under this genus are limited. To gain a comprehensive understanding of its lignocellulose-degrading potential, the whole genome was sequenced using the Illumina NovaSeq 6000 platform. The genome of strain CL21 was assembled into 25 contigs with 3,744,449 bp and a 69.74% GC content and was predicted to contain 3,348 coding genes. Based on taxonomy analysis, strain CL21 shares 73.8 to 82.0% average nucleotide identity with its neighbouring species, below the 95% threshold, indicating its possible status as a distinct species in Nesterenkonia genus. Through in-depth genomic mining, a total of 81 carbohydrate-active enzymes were encoded. Among these, 24 encoded genes were identified to encompass diverse cellulases (GH3), xylanases (GH10, GH11, GH43, GH51, GH127 and CE4), mannanases (GH38 and GH106) and pectinases (PL1, PL9, and PL11). The production of lignocellulolytic enzymes was tested in the presence of several substrates. This study revealed that strain CL21 can produce a diverse array of enzymes which are active at different time points. By combining experimental data with genomic information, the ability of strain CL21 to produce lignocellulolytic enzymes has been elucidated, with potential applications in biorefinery industry.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"71-82"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The CssRS two-component system of Bacillus subtilis contributes to teicoplanin and polymyxin B response. 枯草芽孢杆菌的 CssRS 双组分系统有助于茶青霉素和多粘菌素 B 的反应。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-02-01 Epub Date: 2024-06-07 DOI: 10.1007/s12223-024-01179-8

Alexandr V Kachan, Anatoly N Evtushenkov

{"title":"The CssRS two-component system of Bacillus subtilis contributes to teicoplanin and polymyxin B response.","authors":"Alexandr V Kachan, Anatoly N Evtushenkov","doi":"10.1007/s12223-024-01179-8","DOIUrl":"10.1007/s12223-024-01179-8","url":null,"abstract":"CssRS is a two-component system that plays a pivotal role in mediating the secretion stress response in Bacillus subtilis. This system upregulates the synthesis of membrane-bound HtrA family proteases that cope with misfolded proteins that accumulate within the cell envelope as a result of overexpression or heat shock. Recent studies have shown the induction of CssRS-regulated genes in response to cell envelope stress. We investigated the induction of the CssRS-regulated htrA promoter in the presence of different cell wall- and membrane-active substances and observed induction of the CssRS-controlled genes by glycopeptides (vancomycin and teicoplanin), polymyxins B and E, certain β-lactams, and detergents. Teicoplanin was shown to elicit remarkably stronger induction than vancomycin and polymyxin B. Teicoplanin and polymyxin B induced the spxO gene expression in a CssRS-dependent fashion, resulting in increased activity of Spx, a master regulator of disulfide stress in Bacillus subtilis. The CssRS signaling pathway and Spx activity were demonstrated to be involved in Bacillus subtilis resistance to teicoplanin and polymyxin B.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"83-99"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141283413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0