Folia microbiologica最新文献_第9页

Impacts of ultraviolet and photosynthetically active radiations on photosynthetic efficiency and antioxidant systems of the cyanobacterium Spirulina subsalsa HKAR-19. 紫外线和光合有效辐射对蓝细菌下螺旋藻HKAR-19光合效率和抗氧化系统的影响

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-08-01 Epub Date: 2023-12-02 DOI: 10.1007/s12223-023-01110-7

Jyoti Jaiswal, Neha Kumari, Amit Gupta, Ashish P Singh, Rajeshwar P Sinha

{"title":"Impacts of ultraviolet and photosynthetically active radiations on photosynthetic efficiency and antioxidant systems of the cyanobacterium Spirulina subsalsa HKAR-19.","authors":"Jyoti Jaiswal, Neha Kumari, Amit Gupta, Ashish P Singh, Rajeshwar P Sinha","doi":"10.1007/s12223-023-01110-7","DOIUrl":"10.1007/s12223-023-01110-7","url":null,"abstract":"This study summarizes the response of cyanobacterium Spirulina subsalsa HKAR-19 under simulated light conditions of photosynthetically active radiation (PAR), PAR+UV-A (PA), and PAR+UV-A+UV-B (PAB). Exposure to UV radiation caused a significant (P < 0.05) decrease in chlorophyll a, phycocyanin, and total protein. In contrast, total carotene content increased significantly (P < 0.05) under PA and PAB with increasing irradiation time. The photosynthetic efficiency of photosystem II also decreased significantly in PA and PAB radiation. We have also recorded a decrease in the fluorescence emission intensity of phycocyanin under PA and PAB exposure. The phycocyanin fluorescence shifted towards shorter wavelengths (blue-shift) after 72 h of PA and PAB exposure. Intracellular reactive oxygen species (ROS) levels increased significantly in PA and PAB. Fluorescence microscopic images showed an increase in green fluorescence, indicating ROS generation in UV radiation. We have also quantified ROS generation using green and red fluorescence ratio represented as G/R ratio. A 2-6-fold increase in antioxidative enzymes activity was observed to overcome the damaging effects caused by UV stress as compared to untreated control cultures. The lipid peroxidation was assessed in terms of malondialdehyde content which increases significantly (P < 0.05) as the duration of exposure increases. These results suggest that a combined effect of PAR, UV-A, and UV-B was more deleterious than an individual one.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"747-765"},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138470178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of nitrogen starvation on growth and biochemical composition of some microalgae species. 氮饥饿对某些微藻生长和生化成分的影响。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-08-01 Epub Date: 2024-01-29 DOI: 10.1007/s12223-024-01136-5

Pınar Akdoğan Şirin, Serpil Serdar

{"title":"Effects of nitrogen starvation on growth and biochemical composition of some microalgae species.","authors":"Pınar Akdoğan Şirin, Serpil Serdar","doi":"10.1007/s12223-024-01136-5","DOIUrl":"10.1007/s12223-024-01136-5","url":null,"abstract":"Nitrogen is one of the most important nutrient sources for the growth of microalgae. We studied the effects of nitrogen starvation on the growth responses, biochemical composition, and fatty acid profile of Dunaliella tertiolecta, Phaeodactylum tricornutum, and Nannochloropsis oculata. The lack of nitrogen caused changes in carbohydrate, protein, lipid, and fatty acid composition in all examined microalgae. The carbohydrate content increased 59% in D. tertiolecta, while the lipid level increased 139% in P. tricornutum under nitrogen stress conditions compared to the control groups. Nitrogen starvation increased the oligosaccharide and polysaccharide contents of D. tertiolecta 4.1-fold and 3.6-fold, respectively. Furthermore, triacylglycerol (TAG) levels in N. oculata and P. tricornutum increased 2.3-fold and 7.4-fold, respectively. The dramatic increase in the amount of TAG is important for the use of these microalgae as raw materials in biodiesel. Nitrogen starvation increased the amounts of oligosaccharides and polysaccharides of D. tertiolecta, while increased eicosapentaenoic acid (EPA) in N. oculata and docosahexaenoic acid (DHA) content in P. tricornutum. The amount of polyunsaturated fatty acids (PUFAs), EPA, DHA, oligosaccharides, and polysaccharides in microalgal species can be increased without using the too costly nitrogen source in the culture conditions, which can reduce the most costly of living feeding.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"889-902"},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139570140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Positive effect of inoculation with an Aspergillus strain on phosphorus and iron nutrition plus volatile organic compounds in rice. 接种曲霉菌株对水稻磷、铁营养和挥发性有机化合物的积极影响。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-08-01 Epub Date: 2024-01-10 DOI: 10.1007/s12223-024-01129-4

Boby Vattekkattu Unnikrishnan, Nadayi Karayi Binitha

{"title":"Positive effect of inoculation with an Aspergillus strain on phosphorus and iron nutrition plus volatile organic compounds in rice.","authors":"Boby Vattekkattu Unnikrishnan, Nadayi Karayi Binitha","doi":"10.1007/s12223-024-01129-4","DOIUrl":"10.1007/s12223-024-01129-4","url":null,"abstract":"We explored the potential of a fungal strain Aspergillus costaricensis KS1 for modulating growth and nutrient mobilization in rice. At laboratory conditions, there was a decline in pH of the medium on inoculation with the strain and the production of citric acid was observed under broth conditions. Similarly, there was higher solubilization of tricalcium phosphate and siderophore production in liquid medium on inoculation with the strain. The effect of inoculation of KS1 was studied in rice and higher growth and yield were observed on inoculation compared to control. The content of phosphorus and iron in stem and roots of KS1 inoculated plants was higher in comparison with uninoculated control. There was also increased availability of phosphorus and iron content in soil grown with KS1 inoculated plants. In addition, inoculation with strain resulted in a higher content of volatile organic compounds such as linoleic acid, linolenic acid, and ethyl isoallocholate in stem of rice. A. costaricensis KS1 can be used for improving phosphorus and iron nutrition and impart tolerance against stresses in rice.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"865-874"},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139416780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbiota alteration and its association with immune function in post-COVID-19 patients. 后 COVID-19 患者肠道微生物群的改变及其与免疫功能的关系。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-08-01 Epub Date: 2024-01-05 DOI: 10.1007/s12223-023-01118-z

Jiaojiao Cai, Jingru Xu, Yan Tan, Yao Xiang, Zhifeng Li, Juan Zheng, Yingli Li

{"title":"Gut microbiota alteration and its association with immune function in post-COVID-19 patients.","authors":"Jiaojiao Cai, Jingru Xu, Yan Tan, Yao Xiang, Zhifeng Li, Juan Zheng, Yingli Li","doi":"10.1007/s12223-023-01118-z","DOIUrl":"10.1007/s12223-023-01118-z","url":null,"abstract":"To reveal the variation of gut microbiota and its association with immune function in cured patients with coronavirus 2019 (COVID-19) disease, gut microbiota of patients discharged from hospital for 20 ~ 23 months and healthy volunteers was analyzed by high throughput 16S rRNA sequencing. The diversity and abundance were compared, and the correlation with immunity factors was investigated, and changes in the content of 6 genera microorganisms with proportion higher than 0.1% were revealed in patients with COVID-19 disease: reduced content of Subdoligranulum, Haemophilus, Coprococcus, Eubacterium vertriosum group, and Lachnospiraceae ND3007 group and increased content of Hungatella. NK cells were negatively correlated to Subdoligranulum, while CD8 cells were positively correlated to Subdoligranulum but negative to Hungatella. IL-8 concentration was negatively correlated to Subdoligranulum, Haemophilus, Coprococcus, Eubacterium vertriosum group, and Lachnospiraceae ND3007 group but positively to Hungatella, while IL-1β concentration was negatively correlated to Haemophilus and Eubacterium ventriosum group but positively to Hungatella. The variation of probiotics and potential pathogenic bacteria implies a higher risk in diseases and inflammation, and the modulation of the gut microbiota may help the healing of COVID-19 patients.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"857-864"},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139097704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MSMEG_5850, a global TetR family member supports Mycobacterium smegmatis to survive environmental stress. MSMEG_5850是一个全球性的TetR家族成员，它能支持烟曲霉在环境压力下生存。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-07-17 DOI: 10.1007/s12223-024-01186-9

Parul Singh, Jagdeep Kaur

{"title":"MSMEG_5850, a global TetR family member supports Mycobacterium smegmatis to survive environmental stress.","authors":"Parul Singh, Jagdeep Kaur","doi":"10.1007/s12223-024-01186-9","DOIUrl":"https://doi.org/10.1007/s12223-024-01186-9","url":null,"abstract":"A Mycobacterium smegmatis transcriptional regulator, MSMEG_5850, and its ortholog in M. tuberculosis, rv0775 were annotated as putative TetR Family Transcriptional Regulators. Our previous study revealed MSMEG_5850 is involved in global transcriptional regulation in M. smegmatis and the presence of gene product supported the survival of bacteria during nutritional starvation. Phylogenetic analysis showed that MSMEG_5850 diverged early in comparison to its counterparts in virulent strains. Therefore, the expression pattern of MSMEG_5850 and its counterpart, rv0775, was compared during various in-vitro growth and stress conditions. Expression of MSMEG_5850 was induced under different environmental stresses while no change in expression was observed under mid-exponential and stationary phases. No expression of rv0775 was observed under any stress condition tested, while the gene was expressed during the mid-exponential phase that declined in the stationary phase. The effect of MSMEG_5850 on the survival of M. smegmatis under stress conditions and growth pattern was studied using wild type, knockout, and supplemented strain. Deletion of MSMEG_5850 resulted in altered colony morphology, biofilm/pellicle formation, and growth pattern of M. smegmatis. The survival rate of wild-type MSMEG_5850 was higher in comparison to knockout under different environmental stresses. Overall, this study suggested the role of MSMEG_5850 in the growth and adaptation/survival of M. smegmatis under stress conditions.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabarcoding expands knowledge on diversity and ecology of rare actinobacteria in the Brazilian Cerrado. 元标码扩展了对巴西塞拉多地区稀有放线菌多样性和生态学的了解。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-07-03 DOI: 10.1007/s12223-024-01184-x

Leonardo Bandeira, Christiana Faria, Fernando Cavalcante, Ariel Mesquita, Claudia Martins, Suzana Martins

{"title":"Metabarcoding expands knowledge on diversity and ecology of rare actinobacteria in the Brazilian Cerrado.","authors":"Leonardo Bandeira, Christiana Faria, Fernando Cavalcante, Ariel Mesquita, Claudia Martins, Suzana Martins","doi":"10.1007/s12223-024-01184-x","DOIUrl":"https://doi.org/10.1007/s12223-024-01184-x","url":null,"abstract":"Rare and unknown actinobacteria from unexplored environments have the potential to produce new bioactive molecules. This study aimed to use 16 s rRNA metabarcoding to determine the composition of the actinobacterial community, particularly focusing on rare and undescribed species, in a nature reserve within the Brazilian Cerrado called Sete Cidades National Park. Since this is an inaccessible area without due legal authorization, it is understudied, and, therefore, its diversity and biotechnological potential are not yet fully understood, and it may harbor species with groundbreaking genetic potential. In total, 543 operational taxonomic units (OTUs) across 14 phyla were detected, with Actinobacteria (41.2%), Proteobacteria (26.5%), and Acidobacteria (14.3%) being the most abundant. Within Actinobacteria, 107 OTUs were found, primarily from the families Mycobacteriaceae, Pseudonocardiaceae, and Streptomycetaceae. Mycobacterium and Streptomyces were the predominant genera across all samples. Seventeen rare OTUs with relative abundance < 0.1% were identified, with 82.3% found in only one sample yet 25.5% detected in all units. Notable rare and transient genera included Salinibacterium, Nocardia, Actinomycetospora_01, Saccharopolyspora, Sporichthya, and Nonomuraea. The high diversity and distribution of Actinobacteria OTUs indicate the area's potential for discovering new rare species. Intensified prospection on underexplored environments and characterization of their actinobacterial diversity could lead to the discovery of new species capable of generating innovative natural products.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A pilot study on oral microbiome in electronic cigarettes consumers versus traditional cigarettes smokers. 电子香烟消费者与传统香烟吸烟者口腔微生物组的试点研究。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-07-02 DOI: 10.1007/s12223-024-01185-w

Jilong Liu, Qiulin Yue, Song Zhang, Jing Xu, Xingtao Jiang, Qun Su, Lei Sun, Baojun Li, Kunlun Li, Le Su, Lin Zhao

{"title":"A pilot study on oral microbiome in electronic cigarettes consumers versus traditional cigarettes smokers.","authors":"Jilong Liu, Qiulin Yue, Song Zhang, Jing Xu, Xingtao Jiang, Qun Su, Lei Sun, Baojun Li, Kunlun Li, Le Su, Lin Zhao","doi":"10.1007/s12223-024-01185-w","DOIUrl":"https://doi.org/10.1007/s12223-024-01185-w","url":null,"abstract":"Oral microorganisms are closely related to oral health, the occurrence of some oral diseases is associated with changes in the oral microbiota, and many studies have demonstrated that traditional smoking can affect the oral microbial community. However, due to the short time since the emergence of e-cigarettes, fewer studies are comparing oral microorganisms for users of e-cigarettes versus cigarettes. We collected saliva from 40 non-smokers (NS), 46 traditional cigarette smokers (TS), and 27 e-cigarette consumers (EC), aged between 18 and 35 years. We performed 16S rRNA gene sequencing on the saliva samples collected to study the effects of e-cigarettes versus traditional cigarettes on the oral microbiome. The results showed that compared with the NS group, the alpha diversity of oral flora in saliva was altered in the TS group, with no significant change in the e-cigarette group. Compared with the NS and EC groups, the relative abundance of Actinomyces and Prevotella was increased in the TS group. However, compared with the NS and TS groups, the relative abundance of Veillonella was increased, and the relative abundance of Porphyromonas and Peptostreptococcus was decreased in the EC group. These results showed that both e-cigarettes and traditional cigarettes could alter the structure and composition of oral microbiota. The use of traditional cigarettes promotes the growth of some anaerobic bacteria, which may contribute to dental decay and bad breath over time. E-cigarettes have a different effect on the structure and composition of the oral microbial community compared to conventional cigarettes. In order to better understand the effects of e-cigarettes and traditional cigarettes on users' mouths, future studies will investigate the relationship between diseases such as dental caries and periodontitis and changes in oral microbial species levels.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of co-culture of cellulolytic fungi for enhanced cellulase and xylanase activity and saccharification of untreated lignocellulosic material. 评估纤维素分解真菌共培养对增强纤维素酶和木聚糖酶活性以及对未经处理的木质纤维素材料进行糖化的作用。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-07-02 DOI: 10.1007/s12223-024-01183-y

M Kathirgamanathan, S Weerasinghe, T K Bowange, C L Abayasekara, S A Kulasooriya, R R Ratnayake

{"title":"Evaluation of co-culture of cellulolytic fungi for enhanced cellulase and xylanase activity and saccharification of untreated lignocellulosic material.","authors":"M Kathirgamanathan, S Weerasinghe, T K Bowange, C L Abayasekara, S A Kulasooriya, R R Ratnayake","doi":"10.1007/s12223-024-01183-y","DOIUrl":"https://doi.org/10.1007/s12223-024-01183-y","url":null,"abstract":"Bioethanol production from lignocellulosic materials is hindered by the high costs of pretreatment and the enzymes. The present study aimed to evaluate whether co-cultivation of four selected cellulolytic fungi yields higher cellulase and xylanase activities compared to the monocultures and to investigate whether the enzymes from the co-cultures yield higher saccharification on selected plant materials without thermo-chemical pretreatment. The fungal isolates, Trichoderma reesei F118, Penicillium javanicum FS7, Talaromyces sp. F113, and Talaromyces pinophilus FM9, were grown as monocultures and binary co-cultures under submerged conditions for 7 days. The cellulase and xylanase activities of the culture filtrates were measured, and the culture filtrates were employed for the saccharification of sugarcane leaves, Guinea grass leaves, and water hyacinth stems and leaves. Total reducing sugars and individual sugars released from each plant material were quantified. The co-culture of Talaromyces sp. F113 with Penicillium javanicum FS7 and of T. reesei F118 with T. pinophilus FM9 produced significantly higher cellulase activities compared to the corresponding monocultures whereas no effect was observed on xylanase activities. Overall, the highest amounts of total reducing sugars and individual sugars were obtained from Guinea grass leaves saccharified with the co-culture of T. reesei F118 with T. pinophilus FM9, yielding 63.5% saccharification. Guinea grass leaves were found to be the most susceptible to enzymatic saccharification without pre-treatment, while water hyacinth stems and leaves were the least. Accordingly, the study suggests that fungal co-cultivation could be a promising approach for the saccharification of lignocellulosic materials for bioethanol production.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial activity of gallic acid and methyl gallate against emerging non-fermenting bacilli. 没食子酸和没食子酸甲酯对新出现的非发酵菌的抗菌活性。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-06-21 DOI: 10.1007/s12223-024-01182-z

Orlando Flores-Maldonado, Jorge Dávila-Aviña, Gloria M González, Miguel A Becerril-García, Ana L Ríos-López

{"title":"Antibacterial activity of gallic acid and methyl gallate against emerging non-fermenting bacilli.","authors":"Orlando Flores-Maldonado, Jorge Dávila-Aviña, Gloria M González, Miguel A Becerril-García, Ana L Ríos-López","doi":"10.1007/s12223-024-01182-z","DOIUrl":"https://doi.org/10.1007/s12223-024-01182-z","url":null,"abstract":"Stenotrophomonas maltophilia, Achromobacter xylosoxidans, and Burkholderia cenocepacia are considered emerging pathogens classified as a public health problem due to extensive antimicrobial resistance. Therefore, the discovery of new therapeutic strategies has become crucial. This study aimed to evaluate the antimicrobial activity of gallic acid and methyl gallate against non-fermenting bacteria. The study included five clinical isolates of Stenotrophomonas maltophilia, Achromobacter xylosoxidans, and Burkholderia cenocepacia. The minimum inhibitory concentrations of gallic acid and methyl gallate were determined by the broth microdilution method. Growth curves, metabolic activity, and biofilm formation of each bacterial strain in the presence or absence of phenolic compounds were performed. Finally, the therapeutic efficacy of the compounds was evaluated using an in vivo model. Gallic acid and methyl gallate showed antibacterial activity against bacterial strains in a concentration range of 64 to 256 µg/mL, both compounds reduced bacterial growth and metabolic activity of the strains, even at subinhibitory concentrations. Only, methyl gallate exhibited activity to inhibit the formation of bacterial biofilms. Moreover, gallic acid and methyl gallate increased larval survival by up to 60% compared to 30% survival of untreated larvae in a bacterial infection model in Galleria mellonella. Our results highlight the potential of gallic acid and methyl gallate as therapeutic alternatives for infections by emerging non-fermentative bacteria.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decolourization and detoxification of Reactive Red-195 azo dye by Staphylococcus caprae isolated from textile effluent. 从纺织废水中分离出的 Caprae 葡萄球菌对 Reactive Red-195 偶氮染料的脱醇和解毒作用。

IF 2.6 4区生物学

Folia microbiologica Pub Date : 2024-06-19 DOI: 10.1007/s12223-024-01175-y

Monika Yadav, Asha Lata Singh

{"title":"Decolourization and detoxification of Reactive Red-195 azo dye by Staphylococcus caprae isolated from textile effluent.","authors":"Monika Yadav, Asha Lata Singh","doi":"10.1007/s12223-024-01175-y","DOIUrl":"https://doi.org/10.1007/s12223-024-01175-y","url":null,"abstract":"Azo dyes are used as coloring agent in textile industries at larger scale. As a result, large quantity of dye-enriched waste water is generated which subsequently poses environmental problems. Biological tool involving bacteria having azoreductase enzyme has proved to be more effective and efficient in dye effluent treatment. Current work focuses on Staphylococcus caprae (S. caprae) for degradation and decolorization of Reactive Red-195 (RR-195) azo dye. For this purpose, factors such as pH, temperature, inoculums, carbon and nitrogen sources, and dye concentrations have been optimized for maximum decolorization and degradation. S. caprae (4 mg/mL) efficiently resulted into 90% decolorization of RR-195 dye under static condition at 100 µg/mL concentration, 30 °C and pH 7.0 at a 12-h contact period. FTIR analysis has revealed the formation of new functional groups in the treated dye such as O-H stretch at 3370 cm-1, C-H band stretching at 2928 cm-1, and new band at 1608 cm-1 which specify the degradation of aromatic ring, 1382 and 1118 cm-1 represents desulfonated peaks. Biodegraded metabolites of RR-195 dye such as phenol, 3, 5-di-tert-butylphenol, and phthalic acid have been identified respectively that find industrial applications. Phytotoxicity test has shown non-toxic effects of treated dye on germination of Vigna radiata and Triticum aestivum seeds. Further, antibiotic diffusion assay has confirmed the biosafety of S. caprae.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141418520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0