Folia microbiologica最新文献

{"title":"Molecular characterization, carbohydrate metabolism and tolerance to abiotic stress of Eremothecium coryli endophytic isolates from fruits of Momordica indica.","authors":"Jefferson Brendon Almeida Dos Reis, Mayara Oliveira Sousa Rodrigues, Leila Lourenço Furtado, Clemildo de Sousa Queiroz Júnior, Helson Mario Martins do Vale","doi":"10.1007/s12223-024-01211-x","DOIUrl":"10.1007/s12223-024-01211-x","url":null,"abstract":"<p><p>Yeasts are unicellular fungi that occur in a wide range of ecological niches, where they perform numerous functions. Furthermore, these microorganisms are used in industrial processes, food production, and bioremediation. Understanding the physiological and adaptive characteristics of yeasts is of great importance from ecological, biotechnological, and industrial perspectives. In this context, we evaluated the abilities to assimilate and ferment different carbon sources, to produce extracellular hydrolytic enzymes, and to tolerate salt stress, heavy metal stress, and UV-C radiation of two isolates of Eremothecium coryli, isolated from Momordica indica fruits. The two isolates were molecularly identified based on sequencing of the 18S-ITS1-5.8S-ITS2 region. Our isolates were able to assimilate nine carbon sources (dextrose, galactose, mannose, cellobiose, lactose, maltose, sucrose, melezitose, and pectin) and ferment three (glucose, maltose, and sucrose). The highest values of cellular dry weight were observed in the sugars maltose, sucrose, and melezitose. We observed the presence of hyphae and pseudohyphae in all assimilated carbon sources. The two isolates were also capable of producing amylase, catalase, pectinase, and proteases, with the highest values of enzymatic activity found in amylase. Furthermore, the two isolates were able to grow in media supplemented with copper, iron, manganese, nickel, and zinc and to tolerate saline stress in media supplemented with 5% NaCl. However, we observed a decrease in CFU at higher concentrations of these metals and NaCl. We also observed morphological changes in the presence of metals, which include changes in cell shape and cellular dimorphisms. The isolates were sensitive to UV-C radiation in the shortest exposure time (1 min). Our findings reinforce the importance of endophytic yeasts for biotechnological and industrial applications and also help to understand how these microorganisms respond to environmental variations caused by human activities.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"601-617"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo biocontrol potential of Bacillus plant growth-promoting rhizobacteria against pectinolytic plant pathogens.","authors":"Asmaa Benaissa, Merdia Bestami, Kheira Fellan, Rokaia Benmalek","doi":"10.1007/s12223-024-01214-8","DOIUrl":"10.1007/s12223-024-01214-8","url":null,"abstract":"<p><p>Bacillus is well known for producing a wide range of compounds that inhibit microbial phytopathogens. From this perspective, we were interested in evaluating the biocontrol potential of 5 plant growth-promoting rhizobacteria Bacillus species (PGPR-Bacillus) on 21 microbial pectinolytic plant pathogens isolated from previous studies. Phytopathogenicity and in vivo biocontrol potential of PGPR curative and preventive treatments were investigated from this angle. Overall, the pathogenicity test on healthy tomato, zucchini, and mandarin showed low rot to no symptoms for all PGPR strain culture treatments. Conversely, zucchini pre-treated with PGPR strains B. circulans and B. cereus for 72 h showed no signs of soft rot and remained healthy when in vitro contaminated with phytopathogens (Neisseria cinerea and Pichia anomala). Additionally, the PGPR-Bacillus strains were shown to be effective in mitigating the symptoms of soft rot in tomatoes, zucchini, and oranges using in vivo curative treatment. It is true that the majority of pectinolytic phytopathogenic strains exhibited antibiotic resistance. In vivo tests revealed that PGPR-Bacillus cell culture was effective against plant pathogens. Thus, PGPR-Bacillus can be considered a potential biocontrol agent for pectinolytic plant pathogens.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"689-697"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting virulence of resistant Escherichia coli by the FDA-approved drugs sitagliptin and nitazoxanide as an alternative antimicrobial approach.","authors":"Sara M Eltabey, Ali H Ibrahim, Mahmoud M Zaky, Adel Ehab Ibrahim, Yahya Bin Abdullah Alrashdi, Sami El Deeb, Moustafa M Saleh","doi":"10.1007/s12223-024-01215-7","DOIUrl":"10.1007/s12223-024-01215-7","url":null,"abstract":"<p><p>The spread of multidrug-resistant Escherichia coli in healthcare facilities is a global challenge. Hospital-acquired infections produced by Escherichia coli include gastrointestinal, blood-borne, urinary tract, surgical sites, and neonatal infections. Therefore, novel approaches are needed to deal with this pathogen and its rising resistance. The concept of attenuating virulence factors is an alternative strategy that might lead to low levels of resistance and combat this pathogen. A sub-inhibitory concentration (¼ MIC) of sitagliptin and nitazoxanide was used for phenotypic assessments of Escherichia coli virulence factors such as biofilm production, swimming motility, serum resistance, and protease production. Moreover, qRT-PCR was used to determine the impact of sub-MIC of the tested drugs on the relative expression levels of papC, fimH, fliC, kpsMTII, ompT_m, and stcE genes encoding virulence factors in Escherichia coli. Also, an in vivo model was conducted as a confirmatory test. Phenotypically, our findings demonstrated that the tested strains showed a significant decrease in all the tested virulence factors. Moreover, the genotypic results showed a significant downregulation in the relative expression levels of all the tested genes. Besides, the examined drugs were found to be effective in protecting mice against Escherichia coli pathogenesis. Sitagliptin and nitazoxanide exhibited strong anti-virulence activities against Escherichia coli. In addition, it is recommended that they might function as adjuvant in the management of Escherichia coli infections with either conventional antimicrobial agents or alone as alternative treatment measures.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"645-656"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between the skin microbiome and probiotics in the healing of burn injuries.","authors":"Samane Teymouri, Maryam Pourhajibagher, Abbas Bahador","doi":"10.1007/s12223-025-01262-8","DOIUrl":"10.1007/s12223-025-01262-8","url":null,"abstract":"<p><p>The relationship between the skin microbiome and probiotics in the healing of burn injuries has garnered significant attention in recent years. Burn injuries disrupt the delicate balance of the skin microbiome, leading to complications in the healing process. Probiotic therapies have emerged as promising interventions to restore microbial balance, inhibit biofilm formation, and accelerate tissue repair. Probiotics may also mitigate the risk of antibiotic-resistant infections, which is a major concern in burn units. By enhancing immune responses and stimulating the production of antimicrobial peptides, probiotics can effectively combat bacterial colonization and prevent the emergence of drug-resistant strains. A combination of probiotics with other therapies, such as phages or nanoparticles, holds significant promise for enhancing burn healing. This approach can effectively treat burn wounds by promoting wound healing synergy, preventing infection, modulating the immune response, and disrupting biofilms. Overall, the relationship between the skin microbiome and probiotics in burn wound healing has substantial potential to advance the field of burn wound management.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"535-544"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atrazine dependence in cultivated fungal communities.","authors":"Gessyca Fernanda da Silva, Jose Alejandro Morales Gomez, João Victor Fonseca Moreira, Giulio Mendes Braatz, Rafaella Costa Bonugli-Santos","doi":"10.1007/s12223-024-01204-w","DOIUrl":"10.1007/s12223-024-01204-w","url":null,"abstract":"<p><p>The isolation and study of fungi within specific contexts yield valuable insights into the intricate relationships between fungi and ecosystems. Unlike culture-independent approaches, cultivation methods are advantageous in this context because they provide standardized replicates, specific species isolation, and easy sampling. This study aimed to understand the ecological process using a microcosm system with pesticide concentrations similar to those found in the soil, in contrast to high doses, from the isolation of the enriched community. The atrazine concentrations used were 0.02 mg/kg (control treatment), 300 ng/kg (treatment 1), and 3000 ng/kg (treatment 2), using a 28-day microcosm system. Ultimately, the isolation resulted in 561 fungi classified into 76 morphospecies. The Ascomycota phylum was prevalent, with Purpureocillium, Aspergillus, and Trichoderma being consistently isolated, denoting robust and persistent genera. Diversity analyses showed that the control microcosms displayed more distinct fungal morphospecies, suggesting the influence of atrazine on fungal communities. Treatment 2 (higher atrazine concentration) showed a structure comparable to that of the control, whereas treatment 1 (lower atrazine concentration) differed significantly, indicating that atrazine concentration impacted community variance. Higher atrazine addition subtly altered ligninolytic fungal community dynamics, implying its potential for pesticide degradation. Finally, variations in atrazine concentrations triggered diverse community responses over time, shedding light on fungal resilience and adaptive strategies against pesticides.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"575-587"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virulence potential of Bacillus cereus sensu lato group bacteria isolated from green leafy vegetables.","authors":"Büşra Sultan Çetin, Didem Akpınar Kankaya, Banu Özden Tuncer, Yasin Tuncer","doi":"10.1007/s12223-025-01275-3","DOIUrl":"https://doi.org/10.1007/s12223-025-01275-3","url":null,"abstract":"<p><p>In this study, the virulence potential-including hemolytic activity, toxin gene content, and antibiotic resistance patterns-of Bacillus cereus sensu lato (B. cereus s.l.) group bacteria isolated from green leafy vegetables (mint, parsley, purslane, dill, cress, arugula, lettuce, and baby spinach) was investigated. A total of 59 presumptive B. cereus s.l. bacteria were isolated from 40 vegetable samples. Fourty-six isolates were confirmed as members of the B. cereus s.l. group by polymerase chain reaction (PCR). Species-specific PCR identified 42 of the 46 isolates as B. cereus sensu stricto (B. cereus) and four as B. mycoides. All isolates showed β-hemolytic activity on Columbia sheep blood agar. Except for B. cereus 472 and B. mycoides 571, all isolates harbored between one and eight toxin genes. The most frequently detected toxin gene was entFM, found in 81.0% (34/42) of B. cereus and 75.0% (3/4) of B. mycoides strains. The hblACD and nheABC gene clusters were identified in only 19.1% (8/42) and 7.1% (3/42) of B. cereus strains, respectively. Similarly, the emetic toxin genes ces (9.5%, 4/42) and CER (4.8%, 2/42) were detected exclusively in B. cereus strains. In total, 31 distinct toxin gene profiles were observed. Linezolid and vancomycin were the most effective antibiotics against both B. cereus and B. mycoides strains. However, B. cereus strains exhibited varying degrees of resistance to all tested antibiotics. All B. mycoides strains were resistant to clindamycin, and 75.0% (3/4) were also resistant to ciprofloxacin, imipenem, levofloxacin, meropenem, and norfloxacin. The multiple antibiotic resistance (MAR) index was greater than 0.20 in 60.9% (28/46) of the B. cereus s.l. isolates, and 52.2% (24/46)-including 21 B. cereus and three B. mycoides isolates-exhibited multidrug resistance (MDR). In conclusion, these findings suggest that B. cereus s.l. group isolates from green leafy vegetables may pose a potential risk to public health.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144157462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating Trichoderma viride for enhancing oil palm growth and biochemical defense against fusarium wilt caused by Fusarium oxysporum f. sp. elaeidis.","authors":"Henri Tibo Ambata Ambata, Moïse Ntah A Eyong, Marie Ampère Boat Bedine, Yves Olivier Youassi Youassi, Mylaure Benissa Jiogue, Fabiola Jeannette Ndondoni Dikongue, Séverin Nguemezi Tchameni, Modeste Lambert Sameza","doi":"10.1007/s12223-025-01273-5","DOIUrl":"https://doi.org/10.1007/s12223-025-01273-5","url":null,"abstract":"<p><p>This work aimed to evaluate the antagonist effect of Trichoderma viride against Fusarium oxysporum and its ability to promote growth and resistance defence of oil palm. The antagonistic effect of T. viride was tested by paired culture, cellophane plate, and micro-atmosphere methods. In the nursery, 30-day-old oil palm plants were inoculated with T. viride, and 30 days after growing, the roots of plants were infected with the spores of Fusarium oxysporum. Ninety days after infection, plants were harvested, and a series of morphological (height, fresh root and shoot) and biochemical changes (total chlorophyll, phenol compounds, soluble proteins and oxidative enzymes), which are considered to be part of the plant defence response, were evaluated. Results showed that, in dual culture, T. viride significantly reduced mycelial growth of Fusarium oxysporum and released non-volatile and volatile compounds that inhibited the pathogen. In the nursery, there was a significant reduction in disease impact (severity index and incidence) in oil palm inoculated with T. viride and infected with Fusarium oxysporum. A significant increase in plant root and shoot fresh weights, as well as chlorophyll, soluble proteins, and phenol content, was noticed in comparison with non-inoculated control plants. A significant increase in peroxidase, polyphenol oxidase, and phenylalanine ammonia lyase activities was recorded in inoculated and infected plants, compared to control. There was a significant and positive correlation between disease incidence and the activity of these oxidative enzymes. These findings suggest that these compounds play a vital role in plant defence. T. viride could serve as a biological agent to manage vascular wilt in oil palm. However, analysis of its effects on mass production and field application is needed.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Escherichia marmotae: a multidrug-resistant opportunistic human pathogen - first clinical isolation in the Czech Republic.","authors":"Martin Chmel, Petr Ježek, Renata Šafránková, Zuzana Ileninová, Valentyna Vlasatá, Lucia Mališová","doi":"10.1007/s12223-025-01267-3","DOIUrl":"https://doi.org/10.1007/s12223-025-01267-3","url":null,"abstract":"<p><p>The genus Escherichia comprises seven species, including Escherichia marmotae, described in 2015 from the feces of healthy Himalayan marmots (Marmota himalayana). This species exhibits atypical biochemical properties, such as negative lactose fermentation and indole production. The pathogenic potential of E. marmotae is demonstrated by its ability to invade epithelial cells and the presence of virulence genes. The first human infections with E. marmotae were recorded in Norway in 2021. This article describes the case of a 64-year-old man undergoing chemotherapy for urinary tract cancer, who was hospitalized with febrile neutropenia. An infection caused by E. marmotae and K. pneumoniae was detected. Laboratory diagnostics revealed a multidrug-resistant strain of E. coli producing KPC-type carbapenemase, subsequently identified as E. marmotae by MALDI-TOF MS (Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry) and confirmed by 16S rRNA sequencing and whole genome sequencing. Biochemical tests indicated similarities to E. coli. Virulence analysis revealed the presence of genes typical of pathogenic strains of E. coli. This case report is the first documented instance of E. marmotae in the Czech Republic and highlights the difficulty of distinguishing this species from E. coli in routine diagnostics. Accurate identification requires advanced methods such as MALDI-TOF and sequencing analysis.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pichia Toolkit: Use of the combinatorial library screening system for expression of a marine laccase.","authors":"Igor Vinicius Ramos Otero, Magdalena Haslbeck, Volker Sieber, Lara Durães Sette","doi":"10.1007/s12223-025-01276-2","DOIUrl":"https://doi.org/10.1007/s12223-025-01276-2","url":null,"abstract":"<p><p>Pnh_Lac1 (Lac1) gene from the marine-derived fungus Peniophora sp. CBMAI 1063 was expressed in Pichia pastoris using the Pichia Toolkit system. Constitutive (pGAP, pPET9, pG1, pG6, and pADH2) and methanol-inducible (pAOX1, pDAS1, and pPMP20) promoters were assessed in combination with 21 different signal peptides and His-tag about efficiency in laccase production. Next, 3,200 variants were screened, different culture conditions were evaluated, and an investigation was performed in a bench-scale bioreactor for the best variant selected. The influence of promoters and signal peptides on Lac1 expression was demonstrated in the constitutive libraries. The change from pG6 to pGAP resulted in a 171-fold increase in production. Changing the alpha-mating factor peptide by the native signal peptide of the Lac1 gene decreased laccase production 22-fold. The promoters pGAP (constitutive library) and pAOX1 (inductive library) performed best. The association with the signal peptide αAmylase-αMFD was more efficient for both promoters. The constitutive expression of Lac1 had a 1.37-fold greater production compared to the inducible expression achieved by pAOX1 and was considered more suitable for laccase expression. Culturing the best producer variant pGAP_αA1 at pH 6 and 18 °C resulted in the best production rate in deep-well plates (90 U/L). Constitutive laccase production in a 2-L bioreactor resulted in a peak production of 178 U/L after 78 h. Pichia Toolkit was efficient in the selection of the best molecular regulation and secretion of Lac1. Our findings contribute to the development of marine biotechnology and will serve as the basis for Lac1 production optimization.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight on long non-coding RNA expression profile in THP-derived macrophages infected by Mycobacterium tuberculosis H37Rv, H37Ra, and BCG.","authors":"Shima Hadifar, Abozar Ghorbani","doi":"10.1007/s12223-025-01272-6","DOIUrl":"https://doi.org/10.1007/s12223-025-01272-6","url":null,"abstract":"<p><p>Emerging evidence has suggested a potential role for long non-coding RNAs (lncRNAs) in transcriptome dysregulation during Mycobacterium tuberculosis (Mtb) infection. Understanding the regulatory functions of lncRNAs can provide further insight into the interaction between Mtb and the host. In this study, we sought to explore the lncRNA signature in the Mtb-infected THP1 macrophages (H37Rv, H37Ra, and BCG strains) using the publicly available RNA sequencing dataset of GSE162729. Our analysis identified 6202 putative lncRNAs, with the majority being novel lncRNAs, indicating their significant involvement in the Mtb-infected macrophages. We also identified several differentially expressed lncRNA genes specifically induced in each infected group. Reactome enrichment pathway analysis on cis target genes of lncRNAs revealed that inflammatory immune responses were the predominant features of lncRNAs induced during the H37Rv infection compared to H3Ra and BCG infection. Scavenging by class A receptors and inflammasomes were also highlighted as the common enriched terms among Mtb- and BCG-infected groups. Moreover, we highlighted several potential lncRNAs as hub genes in the predicted regulatory network between the differentially expressed lncRNAs and miRNAs in Mtb-infected THP-1 cells. These findings suggested a possible diverse regulatory role for lncRNAs in the macrophage response to different Mycobacterium strain infections. Further functional study of the lncRNA genes in Mtb infection, while considering the genetic background of the Mtb strain, will be a promising focus for future research.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}