Folia microbiologica最新文献_第6页

Biological activities of citrus fruit-derived copper oxide nanoparticles: towards sustainable antimicrobial and antioxidant solutions. 柑橘类水果衍生的氧化铜纳米颗粒的生物活性：朝着可持续的抗菌和抗氧化解决方案。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-05-10 DOI: 10.1007/s12223-025-01266-4

Bhanu Krishan, Anu Kumar, Wamik Azmi, Sunny Dhiman

{"title":"Biological activities of citrus fruit-derived copper oxide nanoparticles: towards sustainable antimicrobial and antioxidant solutions.","authors":"Bhanu Krishan, Anu Kumar, Wamik Azmi, Sunny Dhiman","doi":"10.1007/s12223-025-01266-4","DOIUrl":"https://doi.org/10.1007/s12223-025-01266-4","url":null,"abstract":"The synthesis of CuO NPs from Citrus fruit peel waste is a noteworthy strategy for the effective repurposing utilization of waste and its application in therapeutic studies. Synthesized copper oxide nanoparticles (CuO NPs) from citrus fruit extracts displayed a dark greenish-black colour with sizes ranging from 379.41, 113.19 and 142.76 nm of lemon, orange and tangerine CuO NPs. Phytochemical screening confirmed the presence of phytochemicals in the extracts wherein lemon CuO NPs lacked flavonoids and cardiac glycosides, while orange CuO NPs lacked alkaloids and flavonoids, and tangerine CuO NPs lacked only alkaloids. The decrease in phenolic concentration in CuO NPs was attributed to complex formation with metal ions. Tangerine CuO NPs exhibited the highest antioxidant activity, while lemon CuO NPs showed the highest total antioxidant capacity. Antibacterial activity increased with CuO NP concentration, with tangerine CuO NPs displaying the highest activity against both Bacillus subtilis subtilis strain 168 and Escherichia coli strain PU-1 isolated from Ghagghar river, Haryana, India. This activity was linked to the disruption of bacterial cell membranes and oxidative stress, supported by the interaction between CuO NPs and bacterial cell components. These findings contribute to understanding of various potential applications of citrus fruit-derived CuO NPs in antimicrobial and antioxidant therapies.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Actinobacillus seminis DnaK interacts with bovine transferrin, lactoferrin, and hemoglobin as a putative iron acquisition mechanism. 半放线杆菌DnaK与牛转铁蛋白、乳铁蛋白和血红蛋白相互作用，作为一种假定的铁获取机制。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-05-10 DOI: 10.1007/s12223-025-01271-7

Candelario Vazquez-Cruz, Edmundo Reyes-Malpica, J Fernando Montes-García, Pamela Bautista-Betancourt, Elena Cobos-Justo, Miguel A Avalos-Rangel, Erasmo Negrete-Abascal

{"title":"Actinobacillus seminis DnaK interacts with bovine transferrin, lactoferrin, and hemoglobin as a putative iron acquisition mechanism.","authors":"Candelario Vazquez-Cruz, Edmundo Reyes-Malpica, J Fernando Montes-García, Pamela Bautista-Betancourt, Elena Cobos-Justo, Miguel A Avalos-Rangel, Erasmo Negrete-Abascal","doi":"10.1007/s12223-025-01271-7","DOIUrl":"https://doi.org/10.1007/s12223-025-01271-7","url":null,"abstract":"Ovine epididymitis, caused by Actinobacilus seminis, is an infectious disease that produces atrophy of the testis, low fertility, and sterility in infected animals. Iron is a microelement necessary for different vital functions in all organisms and most microorganisms. A. seminis iron acquisition mechanisms are undiscovered. For this reason, this work aimed to know the mechanisms this bacterium possesses to respond when grown in an iron restriction culture medium. A. seminis up-expressed three proteins, including a transferrin binding protein, and down-expressed seven (enzymes and putative adhesins) proteins when grown with 2,2'dipyridyl. With chelate, its growth was reduced by 40%, but it was recovered by adding 50-µM FeCl3. No siderophore production was detected with the CAS-BHI medium assay, but siderophore transporter proteins are present. Under normal growth conditions, this microorganism expresses a protein of 70 kDa, identified by mass spectrometry as DnaK. A. seminis DnaK interacts with biotin-labeled transferrin, lactoferrin, or bovine hemoglobin but not with biotin-labeled apo-transferrin or apo-lactoferrin, suggesting its participation in iron acquisition. This protein diminished its expression in iron restriction conditions at 37 °C but remained unchanged at 40 °C, and it is immune recognized by sheep serum with epididymitis. These different iron acquisition mechanisms could give rise to A. seminis, infecting different host tissues.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterisation of fosfomycin resistance genes in Escherichia coli isolates. 大肠杆菌中磷霉素耐药基因的分子特征分析。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-05-07 DOI: 10.1007/s12223-025-01269-1

Funda Yag, Fikriye Milletli Sezgin, Elif Sevim

{"title":"Molecular characterisation of fosfomycin resistance genes in Escherichia coli isolates.","authors":"Funda Yag, Fikriye Milletli Sezgin, Elif Sevim","doi":"10.1007/s12223-025-01269-1","DOIUrl":"https://doi.org/10.1007/s12223-025-01269-1","url":null,"abstract":"Fosfomycin is a well-known antibiotic that exhibits broad-spectrum activity against various bacterial pathogens, including gram-negative strains and some gram-positive strains such as staphylococci. The use of parenteral fosfomycin has been recently revised because the antibiotic has been found to effectively manage serious infections caused by multidrug-resistant pathogens. The occurrence of fosfomycin resistance could threaten the reintroduction of this antibiotic for the treatment of bacterial infections. In this study, a total of 24 fosfomycin-resistant Escherichia coli isolates obtained from urine samples were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin resistance genes. The replication origins of the conjugative and transformant plasmids obtained from the isolates were examined using the replication origin determination method based on the polymerase chain reaction (PCR). Through the PCR process performed with the fosA, fosA3, fosB, fosC, fosC2, and fosX genes to determine fosfomycin resistance, one out of 24 samples was found to be fosA3 gene-positive. A Class-1 integron gene was detected in three fosfomycin-resistant E. coli isolates, while no Class-2 integrons were detected in any isolate. The conjugation experiments demonstrated that the fosA3 gene was transferable in one isolate that also carried the blaTEM, blaCTX-M-15, and aac(6')-ib-cr genes. Through plasmid isolation in the transconjugant E. coli isolates, it was determined that the E. coli isolate FF21 carried fosfomycin resistance on the plasmid. To ensure the continued effective use of fosfomycin as a treatment option, fosfomycin resistance needs to be detected and closely monitored. Given the global rise in plasmid-transmissible genes, we anticipate a growing resistance to fosfomycin in the near future.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of native Bacillus thuringiensis strains possessing nematicidal specific cry genes against Meloidogyne incognita. 原生苏云金芽孢杆菌对丝虫病的特异性杀线虫基因评价。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-05-06 DOI: 10.1007/s12223-025-01268-2

Paramjeet, Devendra Jain, Chandra Prakash Nama, Santosh Ranjan Mohanty

{"title":"Evaluation of native Bacillus thuringiensis strains possessing nematicidal specific cry genes against Meloidogyne incognita.","authors":"Paramjeet, Devendra Jain, Chandra Prakash Nama, Santosh Ranjan Mohanty","doi":"10.1007/s12223-025-01268-2","DOIUrl":"https://doi.org/10.1007/s12223-025-01268-2","url":null,"abstract":"Plant-parasitic nematodes, including root-knot nematodes, are phyto-parasites that cause significant crop damage and economic losses. Bacillus thuringiensis (Bt), which produces nematicidal toxins, is extensively used to combat nematode infestations in agricultural and horticultural crops. This research assessed the efficacy of native Bt strains as a biocontrol agents against the root-knot nematode Meloidogyne incognita. Twenty native Bt strains were evaluated for the presence of nematicidal cry genes using PCR. Eight strains, namely Bt1, Bt5, Bt6, Bt7, Bt17, Bt19, Bt23, and Bt24, exhibited the presence of nematicidal cry genes, specifically cry5, app6, cry12, cry13, cry14, cry21, xpp55, cry31, cry73, and cry40, as determined by gene-specific primers. The in vitro effectiveness of the Bt strains was assessed against M. incognita using a cavity block test, revealing that the Bt strains, namely Bt7 and Bt19, impeded the hatching of M. incognita eggs and were deadly to nematode larvae (J2 stage). SEM analysis of spore-crystal mixtures of Bt isolates revealed different crystal shapes that confirmed the nematicidal activity. Pot experiments revealed that the Bt7 and Bt19 strains are the most efficacious biological agents, exhibiting superior nematicidal activity in Brinjal and Tomato. The molecular characterization of the most virulent Bt strains, namely Bt-7 and Bt-19, using 16S rDNA sequencing, validated their molecular identification as Bacillus thuringiensis.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and activity enhancement of extracellular tyrosinase from a protease-silenced zygomycete Amylomyces rouxii strain. 一株蛋白酶沉默的粘连菌rouxiamylomyces胞外酪氨酸酶的纯化及活性增强。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-05-02 DOI: 10.1007/s12223-025-01264-6

Jaime Marcial-Quino, Francisco J Fernández, Francisco Fierro, Alba M Montiel-González, Araceli Tomasini

{"title":"Purification and activity enhancement of extracellular tyrosinase from a protease-silenced zygomycete Amylomyces rouxii strain.","authors":"Jaime Marcial-Quino, Francisco J Fernández, Francisco Fierro, Alba M Montiel-González, Araceli Tomasini","doi":"10.1007/s12223-025-01264-6","DOIUrl":"https://doi.org/10.1007/s12223-025-01264-6","url":null,"abstract":"The intra- and extra-cellular monophenolase and diphenolase activities of the tyrosinase produced by Amylomyces rouxii were determined in submerged culture using Melin-Norkrans medium supplemented with 12.5 mg/L pentachlorophenol (PCP) and 0.1 g/L tyrosine. Maximal intracellular monophenolase activity was 180 U/mL while maximal extracellular monophenolase activity was 80 U/mL, both using p-cresol as substrate. For diphenolase, the highest intracellular activity was 2233 U/mL using 4-tert-butylcatechol (TBC) as substrate and extracellular diphenolase activity was 975 U/mL with catechol as substrate. The peak tyrosinase activity (mono- and diphenolase) was observed at 48 h of culture. The transformant A412-3 exhibited the highest extracellular activities, with a 2.14-fold increase in monophenolase and a 3.02-fold increase in diphenolase activity compared to the parental strain of A. rouxii. Additionally, it was confirmed that the enzyme secreted was in its active form. Extracellular tyrosinase from the transformant A412-3 was partially purified, achieving a purification factor of 10.6. SDS-PAGE analysis of partially purified tyrosinase revealed three bands of 40, 53, and 130 kDa. These bands were sequenced by LC-MS/MS, revealing eight peptides that showed similarity to tyrosinases from different fungi. It was determined that purified tyrosinase exhibited higher diphenolase activity than monophenolase activity, in line with previous studies on fungal tyrosinases.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The response surface method enables efficient optimization of induction parameters for the production of bioactive peptides in fed-batch bioreactors using Escherichia coli. 响应面法能够有效优化利用大肠杆菌进料间歇式生物反应器生产生物活性肽的诱导参数。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-04-26 DOI: 10.1007/s12223-025-01265-5

Z R Khasanshina, I A Kornakov, E A Buslaeva, R V Drai

{"title":"The response surface method enables efficient optimization of induction parameters for the production of bioactive peptides in fed-batch bioreactors using Escherichia coli.","authors":"Z R Khasanshina, I A Kornakov, E A Buslaeva, R V Drai","doi":"10.1007/s12223-025-01265-5","DOIUrl":"https://doi.org/10.1007/s12223-025-01265-5","url":null,"abstract":"The production of recombinant peptides is critical in biotechnology and medicine for treating a variety of diseases. Thus, there is an urgent need for the development of quick, scalable, and cost-effective recombinant protein expression strategies. This study optimizes induction conditions for an insulin precursor, an analog GLP-1 precursor, and a peptide for COVID-19 therapy expression in E. coli using the response surface method. Factors such as pH, temperature, induction time, isopropyl-β-D-thiogalactopyranoside concentration, and optical density significantly influence peptide productivity. Experimental validation supports the effectiveness of these models in predicting peptide yields under optimal conditions. The optimal induction conditions were determined as follows: temperature at 37 °C, pH of the medium 7.0-8.0, induction at the early logarithmic phase of growth, isopropyl-β-D-thiogalactopyranoside concentration of 0.05 mM, and induction time of 6 h. After model validation, the productivity of each peptide producer exceeded 3 g/L. The optimal conditions achieved peptide titers significantly higher than those previously reported, suggesting that this technique is a versatile cultivation technology for the efficient production of different recombinant peptides. In conclusion, our research enhances the understanding of how tailored cultivation conditions can optimize recombinant peptide production efficiency.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effects of Lactobacillus reuteri strain I300 against Helicobacter pylori adhesion, invasion, and inflammatory response in gastric epithelial cells in vitro. 罗伊氏乳杆菌I300菌株对幽门螺杆菌粘附、侵袭和胃上皮细胞炎症反应的体外抑制作用

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-04-17 DOI: 10.1007/s12223-025-01263-7

Ghazaleh Talebi, Ali Nabavi-Rad, Zahra Sadeghloo, Michael Doulberis, Mohammad Reza Zali, Abbas Yadegar

{"title":"Inhibitory effects of Lactobacillus reuteri strain I300 against Helicobacter pylori adhesion, invasion, and inflammatory response in gastric epithelial cells in vitro.","authors":"Ghazaleh Talebi, Ali Nabavi-Rad, Zahra Sadeghloo, Michael Doulberis, Mohammad Reza Zali, Abbas Yadegar","doi":"10.1007/s12223-025-01263-7","DOIUrl":"https://doi.org/10.1007/s12223-025-01263-7","url":null,"abstract":"The increasing rate of Helicobacter pylori (H. pylori) antibiotic resistance has attenuated the effectiveness of conventional antibiotic-based treatment regimens. This study was aimed at investigating the in vitro inhibitory effects of Lactobacillus reuteri (L. reuteri) strain I300 against H. pylori. The inhibitory effects of live L. reuteri I300 and its different formulations I300L, I300G, and I300T were examined on H. pylori adhesion and invasion to AGS cells. Auto-aggregation and co-aggregation assays and also scanning electron microscopy were performed, evaluating L. reuteri capacity to auto-aggregate and co-aggregate with H. pylori. RT-qPCR and ELISA were used to investigate the expression, and production level of inflammation-related cytokines TNF-α, IL-8, and IL-10. E-cadherin expression level was also measured, determining L. reuteri potential effect on AGS cells integrity. L. reuteri presented a time-dependent capacity to auto-aggregate and co-aggregate with H. pylori. Live L. reuteri and its formulations significantly reduced H. pylori adhesion and invasion of AGS cells. H. pylori treatment with L. reuteri reduced proinflammatory cytokines TNF-α and IL-8 production while increasing anti-inflammatory cytokine IL-10 production. L. reuteri promoted the epithelial cell-cell contact by upregulating E-cadherin expression. This study indicated L. reuteri I300 as a potential probiotic strain with co-aggregation capacity and inhibitory effects against H. pylori adhesion, invasion, and inflammation.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of Megasphaera in the oral microbiome and dyslipidemia onset: evidence from a community-based study in Japan. 巨斑蝶参与口腔微生物组和血脂异常发病：来自日本社区研究的证据。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-04-02 DOI: 10.1007/s12223-025-01258-4

Koki Takagi, Yoshihiro Tamura, Norihiko Narita, Shotaro Komatsu, Shunya Yamazaki, Akihiro Matsumura, Kosei Kubota, Tomoh Matsumiya, Kaori Sawada, Shigeyuki Nakaji, Tatsuya Mikami, Wataru Kobayashi

{"title":"Involvement of Megasphaera in the oral microbiome and dyslipidemia onset: evidence from a community-based study in Japan.","authors":"Koki Takagi, Yoshihiro Tamura, Norihiko Narita, Shotaro Komatsu, Shunya Yamazaki, Akihiro Matsumura, Kosei Kubota, Tomoh Matsumiya, Kaori Sawada, Shigeyuki Nakaji, Tatsuya Mikami, Wataru Kobayashi","doi":"10.1007/s12223-025-01258-4","DOIUrl":"https://doi.org/10.1007/s12223-025-01258-4","url":null,"abstract":"Dyslipidemia is a major risk factor for cardiovascular diseases and is influenced by genetic and environmental factors, including diet. Emerging research suggests a link between the gut microbiome and metabolic disorders. While the connection between the gut microbiota and dyslipidemia is well documented, the specific relationship between oral bacteria and dyslipidemia has not been thoroughly investigated. This study aimed to identify oral bacterial species associated with dyslipidemia in a community-based Japanese population. We conducted a metagenomic analysis on tongue coating samples from 763 participants in the Iwaki Health Promotion Project, which were collected during health checkups in 2017 and 2019. Dyslipidemia was diagnosed using standard lipid level criteria. The oral microbiome was analyzed via 16S rDNA amplicon sequencing. Statistical analyses included multiple regression and β diversity assessments. Our analysis revealed that the abundances of several bacterial genera, including Veillonella, Atopobium, Stomatobaculum, Tanneralla, and Megasphaera, are significantly associated with dyslipidemia. A higher relative abundance of Megasphaera was specifically observed in individuals with dyslipidemia. Moreover, Megasphaera abundance was closely associated with the onset of dyslipidemia (P = 0.038, odds ratio: 1.005, 95% confidence interval: 1.000-1.009), suggesting its role in metabolic regulation. This study revealed a significant association between the abundance of specific oral bacteria and dyslipidemia, suggesting the potential of using the oral microbiota as a biomarker for the early detection and management of dyslipidemia. Future research should explore the mechanisms through which oral bacteria influence lipid metabolism and the potential for microbioma-based therapies.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular identification of lactic acid bacteria from traditional fermented foods and screening exopolysaccharide production by using food wastes. 传统发酵食品中乳酸菌的分子鉴定及利用食品废弃物筛选外多糖的生产。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-04-01 Epub Date: 2024-08-27 DOI: 10.1007/s12223-024-01187-8

Kevser Karaman, Sibel Turan Sirke, Şeyda Nur Türkay Rifaioglu

{"title":"Molecular identification of lactic acid bacteria from traditional fermented foods and screening exopolysaccharide production by using food wastes.","authors":"Kevser Karaman, Sibel Turan Sirke, Şeyda Nur Türkay Rifaioglu","doi":"10.1007/s12223-024-01187-8","DOIUrl":"10.1007/s12223-024-01187-8","url":null,"abstract":"In this study, lactic acid bacteria (LAB) isolation from fermented foods and molecular identification using magnetic bead technology were performed. And then exopolysaccharide (EPS) production possibility was tested in agar medium, and the positive ones were selected for the next step. The bacteria that could produce higher carbohydrate level were grown in MRS medium fortified with whey and pumpkin waste. In our study, 19 different LAB species were identified from fermented products collected from different places in Hatay (Türkiye) province. In molecular identification, universal primer pairs, p806R/p8FPL, and PEU7/DG74 were used for PCR amplification. After that, PCR products purified using paramagnetic bead technology were sequenced by the Sanger sequencing method. The dominant species, 23.8% of the isolates, were identified as Lactiplantibacillus plantarum. As a technological property of LAB, exopolysaccharide production capability of forty-two LAB isolate was tested in agar medium, and after eleven isolates were selected as positive. Two LAB (Latilactobacillus curvatus SHA2-3B and Loigolactobacillus coryniformis SHA6-3B) had higher EPS production capability when they were grown in MRS broth fortified with pumpkin waste and whey. The highest EPS content (1750 mg/L glucose equivalent) was determined in Loigolactobacillus coryniformis SHA6-3B grown in MRS broth fortified with 10% pumpkin waste. Besides the produced EPS samples were validated with FTIR and SEM methods.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"391-401"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142079884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MSMEG_5850, a global TetR family member supports Mycobacterium smegmatis to survive environmental stress. MSMEG_5850是一个全球性的TetR家族成员，它能支持烟曲霉在环境压力下生存。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-04-01 Epub Date: 2024-07-17 DOI: 10.1007/s12223-024-01186-9

Parul Singh, Jagdeep Kaur

{"title":"MSMEG_5850, a global TetR family member supports Mycobacterium smegmatis to survive environmental stress.","authors":"Parul Singh, Jagdeep Kaur","doi":"10.1007/s12223-024-01186-9","DOIUrl":"10.1007/s12223-024-01186-9","url":null,"abstract":"A Mycobacterium smegmatis transcriptional regulator, MSMEG_5850, and its ortholog in M. tuberculosis, rv0775 were annotated as putative TetR Family Transcriptional Regulators. Our previous study revealed MSMEG_5850 is involved in global transcriptional regulation in M. smegmatis and the presence of gene product supported the survival of bacteria during nutritional starvation. Phylogenetic analysis showed that MSMEG_5850 diverged early in comparison to its counterparts in virulent strains. Therefore, the expression pattern of MSMEG_5850 and its counterpart, rv0775, was compared during various in-vitro growth and stress conditions. Expression of MSMEG_5850 was induced under different environmental stresses while no change in expression was observed under mid-exponential and stationary phases. No expression of rv0775 was observed under any stress condition tested, while the gene was expressed during the mid-exponential phase that declined in the stationary phase. The effect of MSMEG_5850 on the survival of M. smegmatis under stress conditions and growth pattern was studied using wild type, knockout, and supplemented strain. Deletion of MSMEG_5850 resulted in altered colony morphology, biofilm/pellicle formation, and growth pattern of M. smegmatis. The survival rate of wild-type MSMEG_5850 was higher in comparison to knockout under different environmental stresses. Overall, this study suggested the role of MSMEG_5850 in the growth and adaptation/survival of M. smegmatis under stress conditions.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"359-370"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0