Extremophiles最新文献_第3页

Untangling the adaptive strategies of thermophilic bacterium Anoxybacillus rupiensis TPH1 under low temperature. 解开嗜热细菌 Anoxybacillus rupiensis TPH1 在低温下的适应策略。

IF 2.6 3区生物学

Extremophiles Pub Date : 2024-07-17 DOI: 10.1007/s00792-024-01346-2

Aditi Mishra, Sindhunath Chakraborty, Tameshwar Prasad Jaiswal, Samujjal Bhattacharjee, Shreya Kesarwani, Arun Kumar Mishra, Satya Shila Singh

{"title":"Untangling the adaptive strategies of thermophilic bacterium Anoxybacillus rupiensis TPH1 under low temperature.","authors":"Aditi Mishra, Sindhunath Chakraborty, Tameshwar Prasad Jaiswal, Samujjal Bhattacharjee, Shreya Kesarwani, Arun Kumar Mishra, Satya Shila Singh","doi":"10.1007/s00792-024-01346-2","DOIUrl":"10.1007/s00792-024-01346-2","url":null,"abstract":"The present study investigates the low temperature tolerance strategies of thermophilic bacterium Anoxybacillus rupiensis TPH1, which grows optimally at 55 °C , by subjecting it to a temperature down-shift of 10 °C (45 °C) for 4 and 6 h followed by studying its growth, morphophysiological, molecular and proteomic responses. Results suggested that although TPH1 experienced increased growth inhibition, ROS production, protein oxidation and membrane disruption after 4 h of incubation at 45 °C yet maintained its DNA integrity and cellular structure through the increased expression of DNA damage repair and cell envelop synthesizing proteins and also progressively alleviated growth inhibition by 20% within two hours i.e., 6 h, by inducing the expression of antioxidative enzymes, production of unsaturated fatty acids, capsular and released exopolysaccharides and forming biofilm along with chemotaxis proteins. Conclusively, the adaptation of Anoxybacillus rupiensis TPH1 to lower temperature is mainly mediated by the synthesis of large numbers of defense proteins and exopolysaccharide rich biofilm formation.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 3","pages":"31"},"PeriodicalIF":2.6,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141633113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymes and biosurfactants of industrial interest produced by culturable fungi present in sediments of Boeckella Lake, Hope Bay, north-east Antarctic Peninsula. 南极半岛东北部希望湾 Boeckella 湖沉积物中可培养真菌产生的具有工业价值的酶和生物表面活性剂。

IF 2.6 3区生物学

Extremophiles Pub Date : 2024-06-22 DOI: 10.1007/s00792-024-01345-3

Elisa Amorim Amâncio Teixeira, Láuren Machado Drumond de Souza, Rosemary Vieira, Juan Manuel Lirio, Silvia Herminda Coria, Peter Convey, Carlos Augusto Rosa, Luiz Henrique Rosa

{"title":"Enzymes and biosurfactants of industrial interest produced by culturable fungi present in sediments of Boeckella Lake, Hope Bay, north-east Antarctic Peninsula.","authors":"Elisa Amorim Amâncio Teixeira, Láuren Machado Drumond de Souza, Rosemary Vieira, Juan Manuel Lirio, Silvia Herminda Coria, Peter Convey, Carlos Augusto Rosa, Luiz Henrique Rosa","doi":"10.1007/s00792-024-01345-3","DOIUrl":"10.1007/s00792-024-01345-3","url":null,"abstract":"This study characterized cultivable fungi present in sediments obtained from Boeckella Lake, Hope Bay, in the north-east of the Antarctic Peninsula, and evaluated their production of enzymes and biosurfactants of potential industrial interest. A total of 116 fungal isolates were obtained, which were classified into 16 genera within the phyla Ascomycota, Basidiomycota and Mortierellomycota, in rank. The most abundant genera of filamentous fungi included Pseudogymnoascus, Pseudeurotium and Antarctomyces; for yeasts, Thelebolales and Naganishia taxa were dominant. Overall, the lake sediments exhibited high fungal diversity and moderate richness and dominance. The enzymes esterase, cellulase and protease were the most abundantly produced by these fungi. Ramgea cf. ozimecii, Holtermanniella wattica, Leucosporidium creatinivorum, Leucosporidium sp., Mrakia blollopis, Naganishia sp. and Phenoliferia sp. displayed enzymatic index > 2. Fourteen isolates of filamentous fungi demonstrated an Emulsification Index 24% (EI24%) ≥ 50%; among them, three isolates of A. psychrotrophicus showed an EI24% > 80%. Boeckella Lake itself is in the process of drying out due to the impact of regional climate change, and may be lost completely in approaching decades, therefore hosts a threatened community of cultivable fungi that produce important biomolecules with potential application in biotechnological processes.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 2","pages":"30"},"PeriodicalIF":2.6,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141440386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mineralogical and microbial characterization of alkali hot spring microbial mats and deposits in Pong Dueat Pa Pae hot spring, Northern Thailand. 泰国北部 Pong Dueat Pa Pae 温泉碱性温泉微生物垫和沉积物的矿物学和微生物特征。

IF 2.6 3区生物学

Extremophiles Pub Date : 2024-06-20 DOI: 10.1007/s00792-024-01343-5

C Sriaporn, S Komonjinda, S Awiphan, S Santitharangkun, C Banjongprasert, M Osathanunkul, B Ramsiri

{"title":"Mineralogical and microbial characterization of alkali hot spring microbial mats and deposits in Pong Dueat Pa Pae hot spring, Northern Thailand.","authors":"C Sriaporn, S Komonjinda, S Awiphan, S Santitharangkun, C Banjongprasert, M Osathanunkul, B Ramsiri","doi":"10.1007/s00792-024-01343-5","DOIUrl":"10.1007/s00792-024-01343-5","url":null,"abstract":"Hot spring environments encompass broad physicochemical ranges, in which temperature and pH account for crucial factors shaping hot spring microbial community and diversity. However, the presence of photosynthetic microbial mats adjacent to boiling hot spring vents, where fluid temperatures extend beyond photosynthetic capability, questions the microbial profiles and the actual temperatures of such adjacent mats. Therefore, this study aims to characterize thermophilic microbial communities at Pong Dueat Pa Pae hot spring using next-generation sequencing, including investigating hot spring mineralogy. Results suggest that Pong Dueat Pa Pae hot spring precipitates comprise mainly silica which also acts as the main preservative medium for microbial permineralization. Molecular results revealed the presence of cyanobacterial and Chloroflexi species in the thick, orange and green subaerial mats surrounding the vents, suggesting the mats would be at least 30 °C cooler than source vents despite constantly receiving geyser splashes. Bacterial abundance was considerably higher than archaeal (97.9% versus 2.1%). Cyanobacterial (mainly Synechococcus and Leptolygbya) and Chloroflexi species (mainly Roseiflexus) accounted for almost half (40.04%) of the bacterial community, while DHVEG-6 and Thaumarchaeota comprised dominant members (> 90%) of the archaeal fraction. This study updates and provides insights into thermophilic microbial community composition and mineralogy of hot springs in Thailand.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 2","pages":"29"},"PeriodicalIF":2.6,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Halobacterium yunchengense sp. nov., Natronomonas amylolytica sp. nov., Halorientalis halophila sp. nov., Halobellus salinisoli sp. nov., halophilic archaea isolated from a saline lake and inland saline soil. 从盐湖和内陆盐碱土壤中分离出的嗜卤古细菌--运城卤杆菌新种（Halobacterium yunchengense sp.nov.）、淀粉单胞菌新种（Natronomonas amylolytica sp.nov.）、卤代卤虫新种（Halorientalis halophila sp.nov.）和盐渍卤虫新种（Halobellus salinisoli sp.nov.）。

IF 2.6 3区生物学

Extremophiles Pub Date : 2024-06-18 DOI: 10.1007/s00792-024-01347-1

Ling Cui, Yao Hu, Xin-Xin Li, Xue Ma, Mu Cheng, Shun Tan, Jing Hou, Heng-Lin Cui

{"title":"Halobacterium yunchengense sp. nov., Natronomonas amylolytica sp. nov., Halorientalis halophila sp. nov., Halobellus salinisoli sp. nov., halophilic archaea isolated from a saline lake and inland saline soil.","authors":"Ling Cui, Yao Hu, Xin-Xin Li, Xue Ma, Mu Cheng, Shun Tan, Jing Hou, Heng-Lin Cui","doi":"10.1007/s00792-024-01347-1","DOIUrl":"10.1007/s00792-024-01347-1","url":null,"abstract":"Four halophilic archaeal strains YCN1T, YCN58T, LT38T, and LT62T were isolated from Yuncheng Salt Lake (Shanxi, China) and Tarim Basin (Xinjiang, China), respectively. Phylogenetic and phylogenomic analyses showed that these four strains tightly cluster with related species of Halobacterium, Natronomonas, Halorientalis, and Halobellus, respectively. The AAI, ANI, and dDDH values between these four strains and their related species of respective genera were lower than the proposed threshold values for species delineation. Strains YCN1T, YCN58T, LT38T, and LT62T could be differentiated from the current species of Halobacterium, Natronomonas, Halorientalis, and Halobellus, respectively, based on the comparison of diverse phenotypic characteristics. The polar lipid profiles of these four strains were closely similar to those of respective relatives within the genera Halobacterium, Natronomonas, Halorientalis, and Halobellus, respectively. The phenotypic, phylogenetic, and genome-based analyses indicated that strains YCN1T, YCN58T, LT38T, and LT62T represent respective novel species within the genera Halobacterium, Natronomonas, Halorentalis, and Halobellus, for which the names Halobacterium yunchengense sp. nov., Natronomonas amylolytica sp. nov., Halorientalis halophila sp. nov., and Halobellus salinisoli sp. nov. are proposed, respectively.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 2","pages":"28"},"PeriodicalIF":2.6,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141418553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and biological activities of a novel exopolysaccharide produced from Geobacillus thermodenitrificans HBB 111 strain. 热核硝化地杆菌 HBB 111 菌株产生的新型外多糖的特性和生物活性。

IF 2.6 3区生物学

Extremophiles Pub Date : 2024-06-11 DOI: 10.1007/s00792-024-01344-4

Mehmet Aytar, Gamze Başbülbül, Deniz Aktaş Uygun

引用次数: 0

Extremophiles in a changing world 变化世界中的嗜极生物

IF 2.9 3区生物学

Extremophiles Pub Date : 2024-04-29 DOI: 10.1007/s00792-024-01341-7

D. A. Cowan, S. V. Albers, G. Antranikian, H. Atomi, B. Averhoff, M. Basen, A. J. M. Driessen, M. Jebbar, Z. Kelman, M. Kerou, J. Littlechild, V. Müller, P. Schönheit, B. Siebers, K. Vorgias

{"title":"Extremophiles in a changing world","authors":"D. A. Cowan, S. V. Albers, G. Antranikian, H. Atomi, B. Averhoff, M. Basen, A. J. M. Driessen, M. Jebbar, Z. Kelman, M. Kerou, J. Littlechild, V. Müller, P. Schönheit, B. Siebers, K. Vorgias","doi":"10.1007/s00792-024-01341-7","DOIUrl":"https://doi.org/10.1007/s00792-024-01341-7","url":null,"abstract":"Extremophiles and their products have been a major focus of research interest for over 40 years. Through this period, studies of these organisms have contributed hugely to many aspects of the fundamental and applied sciences, and to wider and more philosophical issues such as the origins of life and astrobiology. Our understanding of the cellular adaptations to extreme conditions (such as acid, temperature, pressure and more), of the mechanisms underpinning the stability of macromolecules, and of the subtleties, complexities and limits of fundamental biochemical processes has been informed by research on extremophiles. Extremophiles have also contributed numerous products and processes to the many fields of biotechnology, from diagnostics to bioremediation. Yet, after 40 years of dedicated research, there remains much to be discovered in this field. Fortunately, extremophiles remain an active and vibrant area of research. In the third decade of the twenty-first century, with decreasing global resources and a steadily increasing human population, the world’s attention has turned with increasing urgency to issues of sustainability. These global concerns were encapsulated and formalized by the United Nations with the adoption of the 2030 Agenda for Sustainable Development and the presentation of the seventeen Sustainable Development Goals (SDGs) in 2015. In the run-up to 2030, we consider the contributions that extremophiles have made, and will in the future make, to the SDGs.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"61 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140840958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative molecular dynamics simulations provided insights into the mechanisms of cold-adaption of alginate lyases from the PL7 family 比较分子动力学模拟揭示了 PL7 家族藻酸盐裂解酶的冷适应机制

IF 2.9 3区生物学

Extremophiles Pub Date : 2024-04-10 DOI: 10.1007/s00792-024-01340-8

Fei Bian, Xiao-Yue Liang, Meng Wang, Zhong-Zhi Sun, Bin-Bin Xie

{"title":"Comparative molecular dynamics simulations provided insights into the mechanisms of cold-adaption of alginate lyases from the PL7 family","authors":"Fei Bian, Xiao-Yue Liang, Meng Wang, Zhong-Zhi Sun, Bin-Bin Xie","doi":"10.1007/s00792-024-01340-8","DOIUrl":"https://doi.org/10.1007/s00792-024-01340-8","url":null,"abstract":"Alginate is an important polysaccharide that is abundant in the marine environments, including the Polar Regions, and bacterial alginate lyases play key roles in its degradation. Many reported alginate lyases show characteristics of cold-adapted enzymes, including relatively low temperature optimum of activities (Topt) and low thermal stabilities. However, the cold-adaption mechanisms of alginate lyases remain unclear. Here, we studied the cold-adaptation mechanisms of alginate lyases by comparing four members of the PL7 family from different environments: AlyC3 from the Arctic ocean (Psychromonas sp. C-3), AlyA1 from the temperate ocean (Zobellia galactanivorans), PA1167 from the human pathogen (Pseudomonas aeruginosa PAO1), and AlyQ from the tropic ocean (Persicobacter sp. CCB-QB2). Sequence comparison and comparative molecular dynamics (MD) simulations revealed two main strategies of cold adaptation. First, the Arctic AlyC3 and temperate AlyA1 increased the flexibility of the loops close to the catalytic center by introducing insertions at these loops. Second, the Arctic AlyC3 increased the electrostatic attractions with the negatively charged substrate by introducing a high portion of positively charged lysine at three of the insertions mentioned above. Furthermore, our study also revealed that the root mean square fluctuation (RMSF) increased greatly when the temperature was increased to Topt or higher, suggesting the RMSF increase temperature as a potential indicator of the cold adaptation level of the PL7 family. This study provided new insights into the cold-adaptation mechanisms of bacterial alginate lyases and the marine carbon cycling at low temperatures.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140584384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungal diversity present in snow sampled in summer in the north-west Antarctic Peninsula and the South Shetland Islands, Maritime Antarctica, assessed using metabarcoding 利用代谢编码评估南极洲西北部和南设得兰群岛夏季采样的雪中真菌多样性

IF 2.9 3区生物学

Extremophiles Pub Date : 2024-04-04 DOI: 10.1007/s00792-024-01338-2

{"title":"Fungal diversity present in snow sampled in summer in the north-west Antarctic Peninsula and the South Shetland Islands, Maritime Antarctica, assessed using metabarcoding","authors":"","doi":"10.1007/s00792-024-01338-2","DOIUrl":"https://doi.org/10.1007/s00792-024-01338-2","url":null,"abstract":"<h3>Abstract</h3> We assessed the fungal diversity present in snow sampled during summer in the north-west Antarctic Peninsula and the South Shetland Islands, maritime Antarctica using a metabarcoding approach. A total of 586,693 fungal DNA reads were obtained and assigned to 203 amplicon sequence variants (ASVs). The dominant phylum was Ascomycota, followed by Basidiomycota, Mortierellomycota, Chytridiomycota and Mucoromycota. Penicillium sp., Pseudogymnoascus pannorum, Coniochaeta sp., Aspergillus sp., Antarctomyces sp., Phenoliferia sp., Cryolevonia sp., Camptobasidiaceae sp., Rhodotorula mucilaginosa and Bannozyma yamatoana were assessed as abundant taxa. The snow fungal diversity indices were high but varied across the different locations sampled. Of the fungal ASVs detected, only 28 were present all sampling locations. The 116 fungal genera detected in the snow were dominated by saprotrophic taxa, followed by symbiotrophic and pathotrophic. Our data indicate that, despite the low temperature and oligotrophic conditions, snow can host a richer mycobiome than previously reported through traditional culturing studies. The snow mycobiome includes a complex diversity dominated by cosmopolitan, cold-adapted, psychrophilic and endemic taxa. While saprophytes dominate this community, a range of other functional groups are present.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"14 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140584342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-based classification of the family Haloferacaceae and description of five novel species of Halobaculum. 基于基因组的 Haloferacaceae 科分类和 Halobaculum 的五个新物种描述。

IF 2.6 3区生物学

Extremophiles Pub Date : 2024-03-28 DOI: 10.1007/s00792-024-01337-3

Shun Tan, Ling-Rui Zhu, Qing-Ke Zhang, Xin-Yue Dong, Jing Hou, Heng-Lin Cui

{"title":"Genome-based classification of the family Haloferacaceae and description of five novel species of Halobaculum.","authors":"Shun Tan, Ling-Rui Zhu, Qing-Ke Zhang, Xin-Yue Dong, Jing Hou, Heng-Lin Cui","doi":"10.1007/s00792-024-01337-3","DOIUrl":"10.1007/s00792-024-01337-3","url":null,"abstract":"The taxonomic status of some species of Halobellus, Haloferax, Halogranum, and Haloplanus within the family Haloferacaceae was elucidated by phylogenetic, phylogenomic, and comparative genomic analyses. The relative species of each genus should constitute a single species based on the overall genome-related indexes proposed for species demarcation. The cutoff values of AAI (72.1%), ANI (82.2%), and rpoB' gene similarity (90.7%) were proposed to differentiate genera within the family Haloferacaceae. According to these standards, a novel genus related to the genus Halobaculum was proposed to accommodate Halobaculum halophilum Gai3-2 T and Halobaculum salinum NJ-3-1 T. Five halophilic archaeal strains, DT31T, DT55T, DT92T, SYNS20T, and YSMS11T, isolated from a tidal flat and a marine solar saltern in China, were subjected to polyphasic classification. The phenotypic, phylogenetic, phylogenomic, and comparative genomic analyses revealed that strains DT31T (= CGMCC 1.18923 T = JCM 35417 T), DT55T (= CGMCC 1.19048 T = JCM 36147 T), DT92T (= CGMCC 1.19057 T = JCM 36148 T), SYNS20T (= CGMCC 1.62628 T = JCM 36154 T), and YSMS11T (= CGMCC 1.18927 T = JCM 34912 T) represent five novel species of the genus Halobaculum, for which the names, Halobaculum lipolyticum sp. nov., Halobaculum marinum sp. nov., Halobaculum litoreum sp. nov., Halobaculum halobium sp. nov., and Halobaculum limi sp. nov., are proposed.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 2","pages":"22"},"PeriodicalIF":2.6,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140318133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antarctic marine sediment as a source of filamentous fungi-derived antimicrobial and antitumor compounds of pharmaceutical interest. 南极海洋沉积物是丝状真菌产生的具有药用价值的抗菌和抗肿瘤化合物的来源。

IF 2.6 3区生物学

Extremophiles Pub Date : 2024-03-27 DOI: 10.1007/s00792-024-01339-1

Karine Fernandes Camacho, Layssa de Melo Carlos, Suzan Prado Fernandes Bernal, Valéria M de Oliveira, Jorge Luiz Maria Ruiz, Júlia Ronzella Ottoni, Rosemary Vieira, Arthur Neto, Luiz Henrique Rosa, Michel Rodrigo Zambrano Passarini

{"title":"Antarctic marine sediment as a source of filamentous fungi-derived antimicrobial and antitumor compounds of pharmaceutical interest.","authors":"Karine Fernandes Camacho, Layssa de Melo Carlos, Suzan Prado Fernandes Bernal, Valéria M de Oliveira, Jorge Luiz Maria Ruiz, Júlia Ronzella Ottoni, Rosemary Vieira, Arthur Neto, Luiz Henrique Rosa, Michel Rodrigo Zambrano Passarini","doi":"10.1007/s00792-024-01339-1","DOIUrl":"10.1007/s00792-024-01339-1","url":null,"abstract":"Antarctica harbors a microbial diversity still poorly explored and of inestimable biotechnological value. Cold-adapted microorganisms can produce a diverse range of metabolites stable at low temperatures, making these compounds industrially interesting for biotechnological use. The present work investigated the biotechnological potential for antimicrobial and antitumor activity of filamentous fungi and bacteria isolated from marine sediment samples collected at Deception Island, Antarctica. A total of 89 microbial isolates were recovered from marine sediments and submitted to an initial screening for L-glutaminase with antitumoral activity and for antimicrobial metabolites. The isolates Pseudogymnoascus sp. FDG01, Pseudogymnoascus sp. FDG02, and Penicillium sp. FAD33 showed potential antiproliferative action against human pancreatic carcinoma cells while showing no toxic effect on non-tumor cells. The microbial extracts from unidentified three bacteria and four filamentous fungi showed antibacterial activity against at least one tested pathogenic bacterial strain. The isolate FDG01 inhibited four bacterial species, while the isolate FDG01 was active against Micrococcus luteus in the minimal inhibitory concentration of 0.015625 μg mL -1. The results pave the way for further optimization of enzyme production and characterization of enzymes and metabolites found and reaffirm Antarctic marine environments as a wealthy source of compounds potentially applicable in the healthcare and pharmaceutical industry.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 2","pages":"21"},"PeriodicalIF":2.6,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140293235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0