Extremophiles最新文献_第3页

Optimization of cellulase production using Bacillus thuringiensis strain TS-04-A halophilic bacterium. 苏云金芽孢杆菌TS-04-A嗜盐菌生产纤维素酶的优化。

IF 2.2 3区生物学

Extremophiles Pub Date : 2026-03-16 DOI: 10.1007/s00792-026-01423-8

Vaishnavi Balaji, Aparna Ganapathy Vilasam Sreekala, Vinod Kumar Nathan

{"title":"Optimization of cellulase production using Bacillus thuringiensis strain TS-04-A halophilic bacterium.","authors":"Vaishnavi Balaji, Aparna Ganapathy Vilasam Sreekala, Vinod Kumar Nathan","doi":"10.1007/s00792-026-01423-8","DOIUrl":"10.1007/s00792-026-01423-8","url":null,"abstract":"Cellulose-hydrolyzing enzyme, cellulase, has a wide range of importance in many industries such as bioethanol, textiles, and food production. A novel isolate, Bacillus thuringiensis TS-04, from the salterns of Tuticorin, India, was identified through 16 S rDNA sequencing and selected for its superior cellulolytic activity (41.49 IU/mL) compared to other isolates. Optimization using Box-Behnken Design and Response Surface Methodology evaluated the effects of pH, incubation time, and inoculum percentage. The optimized conditions (pH 6.0, 72 h, 2.5% inoculum) yielded a maximum cellulase activity of 50.29 IU/mL, a 1.21-fold increase demonstrating model accuracy (R² = 0.9061) and significance (p < 0.05). The crude cellulolytic enzyme preparation exhibited thermal stability at 37 °C and retained high activity in the presence of 1 M NaCl, confirming its halo-stability. The crude cellulolytic enzyme preparation significantly clarified mixed fruit juice and improved stain removal in detergent formulations. These properties distinguish TS-04 from other Bacillus spp., which typically lack combined high-salt tolerance, high productivity, and a broad multifunctional application range. This study establishes B. thuringiensis TS-04 as a promising candidate for industrial enzyme production under extreme conditions, supporting its potential integration into the food and detergent sectors. Future work will focus on enzyme purification and the scale-up of bioprocesses.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Common capacity for far-red light photosynthesis in a canyon thermophilic freshwater system. 峡谷嗜热淡水系统中远红光光合作用的一般能力。

IF 2.2 3区生物学

Extremophiles Pub Date : 2026-02-21 DOI: 10.1007/s00792-026-01422-9

Ivan J Moreno, Alexander Bogdanov, Brian Palenik

引用次数: 0

Integration of ploidy, Orc1/Cdc6 homolog function, and lysine acetylation with phosphate limitation and UV stress responses of Haloferax volcanii. 火山盐铁倍性、Orc1/Cdc6同源功能和赖氨酸乙酰化与磷酸盐限制和紫外线胁迫响应的整合。

IF 2.2 3区生物学

Extremophiles Pub Date : 2026-02-10 DOI: 10.1007/s00792-026-01420-x

Daniel Gal, Colton Smith, Kelsey Cooper, Ricardo L Couto-Rodríguez, Julie A Maupin-Furlow

{"title":"Integration of ploidy, Orc1/Cdc6 homolog function, and lysine acetylation with phosphate limitation and UV stress responses of Haloferax volcanii.","authors":"Daniel Gal, Colton Smith, Kelsey Cooper, Ricardo L Couto-Rodríguez, Julie A Maupin-Furlow","doi":"10.1007/s00792-026-01420-x","DOIUrl":"10.1007/s00792-026-01420-x","url":null,"abstract":"Haloarchaea thrive in extreme environments where hypersalinity, DNA damage, and nutrient scarcity pose significant stresses. Here, we provide insight into how defined phosphate levels, UV stress, and Orc1/Cdc6 homolog type influence growth and genome dynamics (ploidy) in the model haloarchaeon Haloferax volcanii. During phosphate limitation, cells exhibited reduced growth, heightened UV sensitivity and dynamic changes in chromosome ploidy, with an early log-phase increase followed by a reduction at later growth stages. To uncover underlying mechanisms, we examined the sixteen Orc1/Cdc6 homologs of H. volcanii using bioinformatics, AI-based structural predictions, genetics, site-directed mutagenesis, and biochemical assays. When constitutively expressed, Orc10 and Orc14 were found to increase ploidy, while Orc1 and Orc10 impaired growth in a manner exacerbated by low phosphate and UV stress. Substitutions at the lysine acetylation site within the Walker A motif (Orc1 K165) and conserved DNA-binding residues (R498 in Orc1 and R304 in Orc14) were found to affect Orc/Cdc6 homolog functions. Moreover, a ∆pat2 lysine acetyltransferase mutant was found to exhibit reduced ploidy and a striking phosphate-dependent flocking phenotype during transition to stationary phase. These results highlight the interplay between phosphate availability, chromosomal regulation, and stress adaptation. Together, this study provides insight into mechanisms that promote H. volcanii survival in conditions that induce DNA damage and phosphate limitation.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":"10"},"PeriodicalIF":2.2,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12890983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146156720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic insights into abiotic stress tolerance in polar lichen-associated Sphingomonas sp. PAMC26617 and Sphingomonas sp. PAMC26621. 极地地衣相关Sphingomonas sp. PAMC26617和Sphingomonas sp. PAMC26621非生物胁迫耐受性的基因组分析。

IF 2.2 3区生物学

Extremophiles Pub Date : 2026-02-03 DOI: 10.1007/s00792-026-01419-4

Anamika Khanal, So-Ra Han, Jun Hyuck Lee, Tae-Jin Oh

{"title":"Genomic insights into abiotic stress tolerance in polar lichen-associated Sphingomonas sp. PAMC26617 and Sphingomonas sp. PAMC26621.","authors":"Anamika Khanal, So-Ra Han, Jun Hyuck Lee, Tae-Jin Oh","doi":"10.1007/s00792-026-01419-4","DOIUrl":"10.1007/s00792-026-01419-4","url":null,"abstract":"In this study, Sphingomonas sp. PAMC26617 and Sphingomonas sp. PAMC26621 isolated from rocks of an Arctic lichen, Umbillicaria sp. and Cetraria sp. respectively, were evaluated for their tolerance to abiotic stress of heavy metal(loid)s (HMMs), salt, and osmotic (drought-like) stress at two different temperatures. HMMs resistance protein (CzcC, CzcD, CopB, CopC, CopD, ArsH, and CorC), and osmoprotectant enzymes such as gamma-glutamyl phosphate reductase (ProA), glutamate 5-kinase (ProB), and pyrroline-5-carboxylate reductase (ProC) were identified in both strains during genome analysis. Heavy metal (HM) resistance protein (CorA) was only identified in Sphingomonas sp. PAMC26621. In addition, both strains showed tolerance to CuSO4·5H2O (0.1 mM at 15 °C and 0.25 mM at 25 °C), CoCl2·6H2O (0.25 mM at 15 °C and 0.1 mM at 25 °C), ZnSO4·7H2O (0.1 mM at 15 °C and 25 °C) and Na2HAsO4·7H2O (10 mM at 15 °C and 25 °C), except for Cd(NO3)2·4H2O. Sphingomonas sp. PAMC26617 showed 0.05 mM Cd(NO3)2·4H2O at 15 °C and 0.01 mM at 25 °C and Sphingomonas sp. PAMC26621 showed 0.01 mM Cd(NO3)2·4H2O at 15 °C and 25 °C, respectively. Furthermore, both strains showed tolerance up to 100 mM NaCl and 100 mM D-mannitol at both temperatures. This study is very informative and helpful in understanding the diversity and adaptability of lichen-associated bacteria in harsh environments.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":"9"},"PeriodicalIF":2.2,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Halovenus anhuiensis sp. nov. and Natronomonas salinimetallica sp. nov., two extremely halophilic archaeon isolated from a salt mine. 安徽盐矿嗜盐古菌和金属盐碱钠单胞菌，两种从盐矿中分离出来的极端嗜盐古菌。

IF 2.2 3区生物学

Extremophiles Pub Date : 2026-02-03 DOI: 10.1007/s00792-026-01421-w

Ya-Ling Mao, Aodi Zhang, Tao Hong, Yue Ding, Jingfang Liu, Jing Hou, Heng-Lin Cui, Shaoxing Chen

{"title":"Halovenus anhuiensis sp. nov. and Natronomonas salinimetallica sp. nov., two extremely halophilic archaeon isolated from a salt mine.","authors":"Ya-Ling Mao, Aodi Zhang, Tao Hong, Yue Ding, Jingfang Liu, Jing Hou, Heng-Lin Cui, Shaoxing Chen","doi":"10.1007/s00792-026-01421-w","DOIUrl":"10.1007/s00792-026-01421-w","url":null,"abstract":"Two extremely halophilic archaeal strains, HT40T and LN261T, were isolated from salt crystal samples collected from the Dingyuan Salt Mine in Anhui, China. Strain HT40T was found to possess two copies of the 16 S rRNA gene (rrnA and rrnB), while only one copy was detected in strain LN261T. Phylogenetic analysis based on the 16 S rRNA gene sequences revealed that the rrnA and rrnB genes of HT40T showed high sequence similarities with Halovenus aranensis EB27T (94.73%) and Halovenus carboxidivorans WSH3T (97.42%), respectively. Strain LN261T was clustered with Natronomonas aquatica F2–12T, exhibiting 98.50% sequence similarity. Comparative genomic analysis demonstrated that the average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH), and average amino acid identity (AAI) values between HT40T and current species of the genus Halovenus ranged from 70.2 to 81.8%, 19.4–25.6%, and 62.2–83.8%, respectively. Similarly, the corresponding values between LN261T and current species of the genus Natronomonas were determined to be 75.0–87.3%, 21.2–38.8%, and 67.7–86.4%, respectively. All these values were significantly lower than the recommended thresholds for prokaryotic species delineation. Physiological and biochemical characterization indicated that the optimal growth conditions for HT40T were observed at 42 °C, 4.5 M NaCl, and pH 8.0, whereas LN261T exhibited optimal growth at 37 °C, 3.9 M NaCl, and pH 7.5. Based on the distinct phenotypic and genomic differences from the relevant species, strains HT40T (= MCCC 4K00167T = KCTC 4325T) and LN261T (= MCCC 4K00169T = CGMCC 1.17294T = KCTC 4321T) were proposed to represent novel species within the genera Halovenus and Natronomonas, respectively, for which the name Halovenus anhuiensis and Natronomonas salinimetallica are proposed.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":"8"},"PeriodicalIF":2.2,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of anaerobic thermophilic bacterium Caldisericum insulae sp.nov., the second cultivated representative of the phylum Caldisericota. 厌氧嗜热细菌Caldisericum insule sp.nov的分离与特性研究。Caldisericota门的第二个栽培代表。

IF 2.2 3区生物学

Extremophiles Pub Date : 2026-02-03 DOI: 10.1007/s00792-026-01418-5

Nataliya M Ratnikova, A Y Merkel, A A Novikov, A I Slobodkin

{"title":"Isolation and characterization of anaerobic thermophilic bacterium Caldisericum insulae sp.nov., the second cultivated representative of the phylum Caldisericota.","authors":"Nataliya M Ratnikova, A Y Merkel, A A Novikov, A I Slobodkin","doi":"10.1007/s00792-026-01418-5","DOIUrl":"10.1007/s00792-026-01418-5","url":null,"abstract":"The bacterial phylum Caldisericota has a wide geographical and environmental distribution, but cultivated representatives of this phylum have so far been limited to a single strain. We have isolated a novel anaerobic, thermophilic, fermentative bacterium, strain AR60T from a thermal spring in Sakhalin Island, Russia. Cells of strain AR60T were long rods and tended to form aggregates. Growth was observed between 50 and 70 °C (optimum at 60 °C) and between pH 6.0 and 7.5 (optimum pH 6.5). The isolate was capable for fermentative growth on yeast extract, tryptone and peptone. Thiosulfate, S0, DMSO stimulated growth. The strain contained iso-C17:0 as the major fatty acid. Genome size of strain AR60T was 1.58 Mb; G + C content of the genomic DNA was 34.47%. Phylogenetic analysis revealed that strain AR60T belongs to the genus Caldisericum of the phylum Caldisericota and has 98.85% identity to C. exile. Based on physiological properties and phylogenetic analyses including an average nucleotide identity values and in silico DNA-DNA hybridization, strain AR60T is considered as a new species of the genus Caldisericum. We propose to name strain AR60T (= CGMCC 1.18100T = VKM B-3836T) Caldisericum insulae sp. nov. It is the second cultivated representative of the phylum Caldisericota.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":"7"},"PeriodicalIF":2.2,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Marinilactibacillus sp from Lake Urmia as a novel halophilic source of antibacterial bioactive compounds. 从乌尔米亚湖中提取的海洋活性杆菌作为一种新的抗菌活性化合物来源。

IF 2.2 3区生物学

Extremophiles Pub Date : 2026-01-28 DOI: 10.1007/s00792-025-01416-z

Shabnam Golbouy Daghdari, Azam Jafari, Hussein Eisazadeh, Amir Tukmechi

{"title":"Exploring Marinilactibacillus sp from Lake Urmia as a novel halophilic source of antibacterial bioactive compounds.","authors":"Shabnam Golbouy Daghdari, Azam Jafari, Hussein Eisazadeh, Amir Tukmechi","doi":"10.1007/s00792-025-01416-z","DOIUrl":"10.1007/s00792-025-01416-z","url":null,"abstract":"Isolation of microbial species from extreme environments represents a promising strategy for discovering novel antibacterial compounds. In this study, halophilic bacteria isolated from the water and sediments of hypersaline Lake Urmia were screened for antibacterial activity. Molecular identification based on PCR amplification and 16S rRNA gene sequencing confirmed the isolate as a Marinilactibacillus strain closely related to M. piezotolerans. This strain exhibited strong inhibitory effects against Staphylococcus aureus (MIC 25 µg·mL⁻1), Escherichia coli (MIC 200 µg·mL⁻1), and five methicillin-resistant S. aureus (MRSA) strains (MIC 25–50 µg·mL⁻1; inhibition zones 13–18 mm). Gas chromatography–mass spectrometry analysis revealed several bioactive secondary metabolites, including Di-n-octyl phthalate, Diisooctyl phthalate, and Bis(2-ethylhexyl) phthalate, which may contribute to the observed antibacterial activity. This is the first report describing a bioactive Marinilactibacillus isolate from Lake Urmia sediments, emphasizing hypersaline environments as valuable reservoirs of halophilic bacteria with potential for producing alternative antimicrobial agents.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":"6"},"PeriodicalIF":2.2,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146061115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ filtration reveals salinity-driven dynamics of prokaryotic and eukaryotic communities in the Yinggehai solar saltern. 原位过滤揭示了莺歌海太阳盐沼原核和真核生物群落的盐度驱动动力学。

IF 2.2 3区生物学

Extremophiles Pub Date : 2026-01-16 DOI: 10.1007/s00792-025-01417-y

Zijia Wang, Jian Zhang, Shijie Bai

{"title":"In situ filtration reveals salinity-driven dynamics of prokaryotic and eukaryotic communities in the Yinggehai solar saltern.","authors":"Zijia Wang, Jian Zhang, Shijie Bai","doi":"10.1007/s00792-025-01417-y","DOIUrl":"10.1007/s00792-025-01417-y","url":null,"abstract":"Hypersaline environments harbor unique microbial communities adapted to extreme conditions. Here, we investigated microbial communities across a salinity gradient in the Yinggehai Saltern, southern China’s largest saltern, using 24 in situ filtration samples. The results revealed clear taxonomic shifts with increasing salinity. Bacterial diversity peaked in the primary evaporation ponds (PE), reflecting coexistence of marine and halotolerant taxa at moderate salinity, whereas eukaryotic diversity steadily decreased. Alphaproteobacteria declined along the gradient, while several halotolerant taxa (Puniceicoccus, Thiohalocapsa, Wenzhouxiangella) and potentially novel extremophiles became abundant in high-salinity environments. Chlorellales X exhibited remarkable halotolerance, while Cryptophyta and Dinoflagellata decreased with salinity. Network analysis revealed that salinity constrains ecological interactions: PE exhibited predominantly positive co-occurrence associations (69.55% positive), while higher salinity environments showed increased negative co-occurrence patterns (> 43% negative associations). Random Forest modeling confirmed salinity as the primary driver of the community composition. Our comprehensive analysis of prokaryotic-eukaryotic communities and their interactions provides novel insights into microbial adaptation and ecological dynamics in extreme environments.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":"5"},"PeriodicalIF":2.2,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145988966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and composition of fungal communities in freshwater environments of the Fildes Region, Antarctica. 南极洲菲尔德斯地区淡水环境中真菌群落的多样性和组成。

IF 2.2 3区生物学

Extremophiles Pub Date : 2026-01-06 DOI: 10.1007/s00792-025-01415-0

Chao Dong, Jing Su, Li-Yan Yu, Tao Zhang

{"title":"Diversity and composition of fungal communities in freshwater environments of the Fildes Region, Antarctica.","authors":"Chao Dong, Jing Su, Li-Yan Yu, Tao Zhang","doi":"10.1007/s00792-025-01415-0","DOIUrl":"10.1007/s00792-025-01415-0","url":null,"abstract":"Freshwater environments in polar regions, particularly in Antarctica, remain relatively underexplored regarding microbial biodiversity. In this study, we employed third-generation amplicon sequencing technology targeting full-length ribosomal ITS region to investigate the composition and diversity of fungal communities in 41 freshwater samples (lake water, stream water, and ice-melting water) collected from the Fildes Region in Antarctica. A total of 1,107,738 subsampled reads were obtained and classified into 1,884 operational taxonomic units (OTUs). Among these, 315 OTUs were assigned to Ascomycota, 156 to Basidiomycota, 624 to Chytridiomycota, 61 to Cryptomycota, 23 to Mucoromycota, 1 to Zoopagomycota, and 704 to unclassified fungal phyla. Common fungal genera identified included Phenoliferia, Metschnikowia, Cryolevonia, Linnemannia, and Pseudogymnoascus. Significant differences in fungal community composition were observed among different aquatic habitats. Furthermore, four environmental factors—pH, temperature, salinity, and electrical conductivity—showed significant correlations with the fungal community structure. These findings provide new insights into the fungal diversity of Antarctic freshwater ecosystems and advance our understanding of microbial life in extreme environments.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":"4"},"PeriodicalIF":2.2,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145910804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and functional annotation of Glaciozyma antarctica hypothetical proteins using integrated modeling pipelines. 基于集成建模管道的南极冰原假设蛋白的结构和功能注释

IF 2.2 3区生物学

Extremophiles Pub Date : 2025-12-12 DOI: 10.1007/s00792-025-01412-3

Fatin Izzati Abdul Hadi, Doris Huai Xia Quay, Nor Muhammad Mahadi, Mohd Firdaus-Raih, Su Datt Lam

{"title":"Structural and functional annotation of Glaciozyma antarctica hypothetical proteins using integrated modeling pipelines.","authors":"Fatin Izzati Abdul Hadi, Doris Huai Xia Quay, Nor Muhammad Mahadi, Mohd Firdaus-Raih, Su Datt Lam","doi":"10.1007/s00792-025-01412-3","DOIUrl":"10.1007/s00792-025-01412-3","url":null,"abstract":"Glaciozyma antarctica is a psychrophilic yeast that can withstand extreme temperatures by adapting several mechanisms, such as preventing the formation of ice around the cell, maintaining the flexibility of the membrane, preventing the formation of reactive oxygen species, and producing cold-adapted enzymes. The G. antarctica genome encodes 7857 protein sequences, however, 2911 are hypothetical with no annotated functions and only five proteins have been experimentally characterised with structure coordinates in the Protein Data Bank (PDB). In this work, we modelled the of G. antarctica hypothetical proteins (HPs) using the FunMod pipeline and ColabFold, and carried out structural characterisation of the resulting models. We have successfully modelled 1517 (52%) of the hypothetical proteins with good quality structures with an average MolProbity score of 2.6. By integrating annotations from both sequence-based (InterProScan and CATH-Gene3D Functional Family), structure-based (Foldseek) methods, and removing overlapping annotated protein IDs, we predicted functions or domains to a total of 1866 (64%) unique HPs. From this annotated set, 814 proteins (44%) were predicted as probable enzymes, based on keyword parsing of their assigned functions (e.g., ‘enzyme’ or ‘-ase’). The remaining 1052 proteins (56%) were assigned non-enzymatic functions. Notably, several enzymes, including lipases and cellulases, have potential industrial applications such as detergent formulation, while increasing our understanding of adaptation mechanisms in cold temperatures. All generated data is available in the G. antarctica protein structure database as a resource of information for future research on psychrophilic organisms, especially G. antarctica.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":"2"},"PeriodicalIF":2.2,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145741624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0