Halobellus marinus sp. nov., Halobellus ordinarius sp. nov., Halobaculum rarum sp. nov., and Halorarum halobium sp. nov., halophilic archaea isolated from marine solar salt and a saline lake.

IF 2.6 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Extremophiles Pub Date : 2025-01-27 DOI:10.1007/s00792-025-01379-1

Ling-Rui Zhu, Chidiebele Nwankwo, Jing Hou, Heng-Lin Cui

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引用次数: 0

Abstract

Four halophilic archaeal strains were isolated from sea salt and a saline lake in China. Based on phylogenetic and phylogenomic analyses, the four strains are related to the genera of Halobellus, Halobaculum, and Halorarum within the family Haloferacaceae. The four strains possess genes responsible for carotenoid synthesis, maintenance of a high internal salt concentration, as well as diverse enzymes with biotechnological potential. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values among these four strains and their related species were lower than the established thresholds proposed for species demarcation. Strains DFY28^T, ZY16^T, QDC11^T, and XH14^T were distinguished from related species based on a variety of phenotypic characteristics. The major polar lipids of these four strains were similar to those of respective relatives within the genera Halobellus, Halobaculum, and Halorarum. The phenotypic, phylogenetic, and genome-based analyses suggest that strains DFY28^T (= CGMCC 1.17470^T = JCM 34310^T), ZY16^T (= CGMCC 1.17476^T = JCM 34311^T), QDC11^T (= MCCC 4K00127^T = KCTC 4308^T), and XH14^T (= CGMCC 1.17028^T = JCM 34145^T) represent four novel species of the genera Halobellus, Halobaculum and Halorarum, for which the names Halobellus marinus sp. nov., Halobellus ordinarius sp. nov., Halobaculum rarum sp. nov., and Halorarum halobium sp. nov. are proposed.

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从海洋太阳盐和盐湖中分离出的嗜盐古菌——海洋盐藻、普通盐藻、稀有盐藻和盐藻。

从中国某盐湖和海盐中分离到4株嗜盐古细菌。根据系统发育和系统基因组分析，这4株菌株与盐藻科盐藻属（Halobellus）、盐藻属（Halobaculum）和盐藻属（Halorarum）有关。这四种菌株具有负责类胡萝卜素合成、维持高内盐浓度以及具有生物技术潜力的多种酶的基因。这4种菌株及其近缘种的平均核苷酸同源性（ANI）、平均氨基酸同源性（AAI）和数字DNA-DNA杂交（dDDH）值均低于物种划分的既定阈值。根据多种表型特征将菌株DFY28T、ZY16T、QDC11T和XH14T与亲缘种区分开来。这4个菌株的主要极性脂质与Halobellus属、Halobaculum属和Halorarum属的亲缘菌相似。表型、系统发育和基因组分析表明，菌株DFY28T （= CGMCC 1.17470T = JCM 34310T）、ZY16T （= CGMCC 1.17476T = JCM 34311T）、QDC11T （= MCCC 4K00127T = KCTC 4308T）和XH14T （= CGMCC 1.17028T = JCM 34145T）代表了Halobellus、Halobellus marinus sp. nov、Halobellus ordinarius sp. nov、halobellum rarum sp. nov)和halobellum halobarium sp. nov的4个新种。

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来源期刊

Extremophiles 生物-生化与分子生物学

CiteScore

6.80

自引率

6.90%

发文量

审稿时长

2 months

期刊介绍： Extremophiles features original research articles, reviews, and method papers on the biology, molecular biology, structure, function, and applications of microbial life at high or low temperature, pressure, acidity, alkalinity, salinity, or desiccation; or in the presence of organic solvents, heavy metals, normally toxic substances, or radiation.