Biological treatment of methyl orange dye and textile wastewater using halo-alkaliphilic bacteria under highly alkaline conditions.

Abstract

As the textile wastewater is highly saline and has high pH it is important to employ extremophilic microbes to survive in harsh conditions and provide effective bioremediation of textile dyes. This study aims to find a sustainable solution for dye removal by investigating the potential of an indigenously isolated bacterium, Nesterenkonia lacusekhoensis EMLA3 (halo-alkaliphilic) for treatment of an azo dye, methyl orange (MO) and textile effluent. MO dye decolorization studies were conducted using mineral salt media (MSM) by varying incubation time (0-120 h), initial dye concentration (50-350 mg/L), pH (7.0-12.0), inoculum dose (3-10%), agitation (stationary, 100 rpm and 200 rpm), and temperature (20-55 °C). Dye removal by the bacterium for 50 mg/L of dye was > 97.0% within 72 h of incubation at pH 11.0 in stationary condition. Bacterium had excellent reusability i.e. > 97% dye removal for up to 5 cycles. Moreover, bacterium has the potential for co-removal of chromium (VI) (3.5-28 mg/L), and also almost complete dye removal in presence of high amount of NaCl. Liquid chromatography-mass spectrometry showed degradation as the mechanism of dye removal. Application of the bacterium to MO dye spiked real textile wastewater showed excellent dye removal. Phyto-toxicity assessment conducted on Vigna radiata and Triticum aestivum seeds, showed 100% germination of biotreated textile wastewater indicating its reuse potential.