Food and Agricultural Immunology最新文献

{"title":"Different iron sources affected haemoglobin and myoglobin synthesis, and gene expression related to iron metabolism in skeletal muscle in piglets","authors":"Ping Kang, Jiajun Fan, Qilong Xu, Dianchao Gu, Bingzhao Shi, Dan Wang, Huiling Zhu, Jiangchao Zhao, Yulan Liu","doi":"10.1080/09540105.2023.2247182","DOIUrl":"https://doi.org/10.1080/09540105.2023.2247182","url":null,"abstract":"ABSTRACT This study was carried out to investigate the effect of different iron sources on haemoglobin and myoglobin (Mb) synthesis, and the mRNA expression of the key genes related to iron metabolism in the skeletal muscle of piglets. Forty-eight piglets were assigned into four treatments including control group, ferrous sulphate (FeSO4) group, ferrous glycinate (Fe-Gly) group and amino acid-Fe(II)-chelator complexes group. The results showed that iron supplementation increased Mb concentration in muscles (P < 0.05). In addition, iron supplementation increased hephaestin (HEPH) expression in the longissimus dorsi muscle (P < 0.1), decreased transferrin receptor 1 (TFR1) expression in the longissimus dorsi muscle (P < 0.05) and increased lipocalin 2 (Lcn2) expression in gastrocnemius muscle, respectively (P < 0.05). In summary, compared with high-dose inorganic iron, low-dose organic iron supplements have the same effects in haemoglobin and myoglobin biosynthesis, and mobilize iron in muscles into circulation.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41764616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulation of RAW264.7 cells by CP80-1, a polysaccharide of Cordyceps cicadae, via Dectin-1/Syk/NF-κB signaling pathway","authors":"Jingqiong Wan, Yiling Zhu, Xue Jiang, Feixuan Wang, Zhaoyong Zhou, Jibiao Wang, Chang Liu, Yuan Wei, Ouyang Zhen","doi":"10.1080/09540105.2023.2231172","DOIUrl":"https://doi.org/10.1080/09540105.2023.2231172","url":null,"abstract":"ABSTRACT Cordyceps cicadae is a rare edible and medicinal entomogenous fungus. Here, the structural characteristics and immunomodulatory activity of C. cicadae polysaccharide CP80-1 were preliminarily elucidated. It showed that CP80-1 of 250.431 kDa was mainly composed of glucose, xylose and rhamnose. Physiologically, CP80-1 can regulate the immune of RAW264.7 cells by promoting the proliferation, phagocytosis, and secretion of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)−1β, and interleukin (IL)−6. However, these effects were significantly attenuated when Dectin-1 receptor expression was inhibited by laminarin. Moreover, laminarin down-regulated the mRNA levels of relevant genes, inhibited CP80-1-driven expression of proteins related to spleen tyrosine kinase (Syk) and nuclear factor kappa-B (NF-κB) signaling pathway. Additionally, CP80-1 induced the nuclear translocation of NF-κB subunit p65 in RAW264.7 cells. These results suggest that CP80-1 may activate RAW264.7 cells by regulating the Dectin-1/Syk/NF-κB signaling pathway, which provides theoretical basis for the development of C. cicadae in immunomodulation.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46938411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms and biomarkers underlying the protective roles of the nutraceutical laminarin against ischaemic strokes","authors":"Jiefeng Luo, Yuan Lan, Wenwen Pan, Kengpo Lai, Deyan Kong","doi":"10.1080/09540105.2023.2249265","DOIUrl":"https://doi.org/10.1080/09540105.2023.2249265","url":null,"abstract":"ABSTRACT Stroke is the second leading cause of death and a major cause of disability worldwide. Ischemic stroke accounts for approximately >60% of all stroke, and >80% of strokes can be prevented. Middle cerebral artery occlusion (MCAO) is a common cause of stroke in humans. MCAO is associated with blood vessel-related problems, such as reduced vascular plasticity and hypertension. These problems can be attenuated by the use of nutraceuticals such as laminarin. Laminarin is a storage glucan commonly found in brown algae. Accumulating evidence and studies have revealed that laminarin is a promising candidate drug for treating ischaemic stroke. However, details on pharmaceutical targets and molecular mechanisms underlying laminarin’s beneficial effects for treating ischaemic stroke remains largely unknown. Herein, we applied network pharmacology, bioinformatic analysis, and middle cerebral artery occlusion model to delineate the protective role of laminarin. By comparing the laminarin- and MCAO-associated genes, we identified 23 potential targets including tumour necrosis factor (TNF), vascular endothelial growth factor A, selectin P, presenilin 1, fibroblast growth factor 2, microtubule-associated protein tau, caspase 3, matrix metallopeptidase 1, 5-hydroxytryptamine receptor 2A, telomerase reverse transcriptase, interleukin 2, signal transducer and activator of transcription 3 (STAT3), ATP binding cassette subfamily B member 1, catalase, superoxide dismutase 2, adenosine A1 receptor, adenosine A2a receptor, 5-hydroxytryptamine receptor 1B, heat shock protein 90 alpha family class A member 1, matrix metallopeptidase 8, BCL2-like 1, galectin 3, and epoxide hydrolase 2 of laminarin against ischaemic stroke. The gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes enrichment analyses highlighted the importance of this gene cluster in the development of blood vessels, neuronal cell death, brain functions, and neuroinflammation. Furthermore, molecular docking analysis suggested a direct binding of laminarin to its target proteins STAT3 and TNF. Our results provide the pharmaceutical targets and delineate the details regarding the molecular mechanisms underlying the beneficial effects of laminarin against ischaemic stroke. Moreover, our findings support those of previous studies suggesting laminarin as a promising drug for treating ischaemic stroke.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42908422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paecilomyces cicadae: a systematic overview of the biological activities and potential mechanisms of its active metabolites","authors":"Feiqian Di, Jiachan Zhang, Wenjing Cheng, Li Luyao, Li Meng, Changtao Wang","doi":"10.1080/09540105.2023.2243059","DOIUrl":"https://doi.org/10.1080/09540105.2023.2243059","url":null,"abstract":"ABSTRACT\u0000 The microbe Paecilomyces cicadae (Miquel) Samson (P. cicadae) is an asexual strain of Cordyceps cicadae (C. cicadae) used in traditional Chinese medicine. Due to the abundance of secondary metabolites found in cultured metabolites, including polysaccharides, hyaluronic acid, ergosterol peroxide, and active nucleosides, P. cicadae and/or C. cicadae exhibit several therapeutic activities, including immunoregulatory, antioxidant, and anti-diabetic effects. Given the great attention for health care and the desirable medical value of C. cicadae and/or P. cicadae, they are becoming potential alternatives of Cordyceps sinensis (C. sinensis). Although there are many studies on P. cicadae/C. cicadae, most of them are experimental research articles. There are limited reviews to summarize the latest researches on P. cicadae/C. cicadae. This review mainly focuses on the metabolites, physiological activities and mechanisms of action of P. cicadae and/or C. cicadae. This review can serve as a theoretical basis for the more efficient use of this medicinal fungus.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47372774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annotation of genes involved in gamma-aminobutyric multiplication during coix germination by metabolome and transcriptome analysis","authors":"Xiao Zhang, Yuqin Ding, Yi Hao, Guoqing Dong, D. Du, D. Huo","doi":"10.1080/09540105.2023.2241668","DOIUrl":"https://doi.org/10.1080/09540105.2023.2241668","url":null,"abstract":"ABSTRACT Job’s tears (coix) is a widely used grain with rich and balanced nutritional value. In this study, a total of 71 metabolites, including amino acid metabolism, sugar metabolism and fatty acid metabolism, were significantly increased in the germinating coix through liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Significant increase in gamma-aminobutyric (GABA) content during seed germination was detected in the metabolites detected. Transcript profiles generated through high-throughput RNA-sequencing data and 2431 differentially expressed genes (DEGs) were identified. The functional of DEGs were also significantly clustered in glucose metabolism and fatty acid metabolism. In the annotation of metabolite variation and differentially expressed genes, 23 regulatory genes and 10 significantly enriched metabolites for GABA content proliferation during coix germination were identified by association analysis. These results provided a theoretical basis for understanding the germination mechanism of coix and promoting its production and application.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48954577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the mechanism of Lu’an GuaPian tea in treating heart failure based on network pharmacology and molecular docking","authors":"Fang Hua, Ling-li Shi, Pengcheng Zhou","doi":"10.1080/09540105.2023.2231173","DOIUrl":"https://doi.org/10.1080/09540105.2023.2231173","url":null,"abstract":"ABSTRACT\u0000 Objective The study was to investigate the possible mechanism of Lu'an GuaPian tea in treating heart failure (HF) via network pharmacology and molecular docking. Method The target of Lu'an GuaPian tea were screened. The GeneCards, NCBI, and DisGeNET databases were searched for HF targets. The GO and KEGG pathway analysis were used to obtain the targets and pathways. Molecular docking was used to verify the most likely targets and flavonoids. Results The Venn diagram revealed that AKT1, TNF, SRC, EGFR, ESR1, PTGS2, MMP9, and KDR were the top 8 target genes. GO and KEGG analysis suggested that Lu'an GuaPian tea could treat HF through PI3K-Akt pathway. Molecular docking predicted that compounds 1 and 7 have the highest binding affinity. Conclusion Lu'an GuaPian tea can be used to treat HF via PI3K-Akt signaling pathway, and the compound 1 and 7 were the most active lead compounds for regulating PI3K.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45768699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium butyrate alleviates potential Alzheimer’s disease in vitro by suppressing Aβ and tau activation and ameliorates Aβ-induced toxicity","authors":"A. B. Bayazid, Yeong Hwan Jeong, Soo Ah Jeong, B. Lim","doi":"10.1080/09540105.2023.2234100","DOIUrl":"https://doi.org/10.1080/09540105.2023.2234100","url":null,"abstract":"ABSTRACT The number of Alzheimer’s disease (AD) affected patients is increasing without any effective cure and the etiology remains to be understood. Inflammations, oxidative stress, Aβ, and tauopathy are associated factors of AD. Sodium butyrate (NaB) is an HDAC inhibitor profoundly found to be neuroprotective. We have investigated the neuroprotective effects of NaB in SH-SY5Y cells stimulated with TNF-α/Aβ in SH-SY5Y cells and LPS-induced BV-2 cells. The cell viability and NO production were also investigated by MTT and Griess reagent assay. The expressions of APP/BACE, Aβ, and tau phosphorylation and the apoptotic regulators, including P-53, and caspase-1 were analysed by western blot analysis. Our findings exerted that NaB ameliorated cell death and inhibited NO production in Aβ-induced SH-SY5Y cells and LPS in BV-2 cells. NaB notably decreased the expression of tau hyperphosphorylation in TNF-α-stimulated SH-SY5Y and LPS-induced BV-2 cells. NaB remarkably attenuated APP/BACE and Aβ expressions in TNF-α-induced SH-SY5Y cells. Cell viability was restored by NaB and downregulated apoptotic proteins p-53, caspase-1 level in aggregated Aβ-induced SH-SY5Y cells. NaB increased Nrf-2/HO-1 expressions and substantially reversed the reactive oxygen species in Aβ-induced SH-SY5Y cells. Altogether, our results suggest that NaB could be a potential therapeutic agent against AD. GRAPHICAL ABSTRACT","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47445335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phillyrin attenuates airway inflammation and Th2 cell activities in a mouse asthma model","authors":"Wen-Chung Huang, Shu-Ju Wu, Li-Wen Fang, Tzu-Yung Lin, Chian-Jiun Liou","doi":"10.1080/09540105.2023.2231182","DOIUrl":"https://doi.org/10.1080/09540105.2023.2231182","url":null,"abstract":"ABSTRACT Phillyrin is isolated from the fruit of Forsythia suspensa, and exhibits multiple pharmacological effects, including anti-tumor, anti-inflammation, and anti-oxidation activities. Here, we investigated whether phillyrin could alleviate airway hyperresponsiveness (AHR) and eosinophil infiltration in the lungs of asthmatic mice, and mitigate inflammatory responses in tracheal epithelial BEAS-2B cells. IL-4/TNF-α-stimulated BEAS-2B cells were treated with various phillyrin doses. Female BALB/c mice were sensitised and challenged with ovalbumin (OVA), and then treated with intraperitoneal injection different phillyrin doses. In IL-4/TNF-α-stimulated BEAS-2B cells, phillyrin effectively reduced proinflammatory cytokines, chemokines, and eotaxin (CCL11) levels. In the lungs of asthmatic mice, phillyrin treatment relieved AHR, airway inflammation, eosinophil infiltration, and goblet cell hyperplasia. Phillyrin also reduced serum OVA-IgE, and Th2-associated cytokine levels in splenocyte culture medium, and in bronchoalveolar lavage fluid of asthmatic mice. Our results indicate that phillyrin attenuated airway inflammation and eosinophil infiltration in asthmatic mice by suppressing Th2 cytokine production.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"1 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41761136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IFN-γ mediated neuroinflammation and behaviour abnormality after maternal exposure to low dose mixture of food additives and pesticides","authors":"Linyang Song, Xiaoyi He, Mengxia Wang, Lixia Li, Jing Liu, Guoying Li, Junhua Yang","doi":"10.1080/09540105.2023.2226841","DOIUrl":"https://doi.org/10.1080/09540105.2023.2226841","url":null,"abstract":"ABSTRACT People often simultaneously expose to acceptable/tolerable daily intake of food additives and pesticides in daily life. Exposure in adult rodents to acceptable/tolerable daily intake of food additives and pesticides chemical mixtures (AFACM) is reported to cause health damage. Our recently study reported that AFACM exposure of pregnant mice resulted in neuroinflammation and abnormal emotional behaviours in offspring. Here, we found that neutralising IFN-γ with neutralising antibodies completely blocked the influences induced by maternal AFACM exposure, including the increased IFN-γ level in serum, increased IFN-γ, interleukin-6 and tumour necrosis factor-α levels in prefrontal cortex(PFC) in offspring on postnatal day(PND)7, the abnormal emotional behaviours in offspring on PND56, and the decreased of ATPase phospholipid flippase 8A2(ATP8A2) in the PFC of offspring on PND7. In sum, maternal AFACM exposure induced the PFC neuroinflammation, abnormal emotional behaviours and decreased PFC ATP8A2 expression through increasing systemic IFN-γ in offspring.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42783017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes derived from berberine-treated bone marrow mesenchymal stem cells ameliorate inflammatory arthritis in rats with collagen-induced rheumatoid arthritis","authors":"Fei Sun, Yuxia Ma, Fa-Xin Li, Congcong Li, Zhaowen Yang","doi":"10.1080/09540105.2023.2220566","DOIUrl":"https://doi.org/10.1080/09540105.2023.2220566","url":null,"abstract":"ABSTRACT To evaluate the effect of exosomes secreted by berberine-treated bone marrow-derived mesenchymal stem cells (BMSCs) on synovial inflammation in collagen-induced arthritis (CIA) model. Exosomes were isolated from berberine-treated BMSCs (Ber-BMSC-EXs) and untreated BMSCs (BMSC-EXs). CIA rats were developed and treated with Ber-BMSC-EXs or BMSC-EXs. Clinical arthritis index was measured, activation of fibroblast-like synoviocytes (FLSs) were evaluated, and response and frequency of T helper 17 (Th17) and regulatory T (Treg) cells as well as cytokine levels in synovial fluid were determined. Ber-BMSC-EXs (with a superior effect than BMSC-EXs) significantly alleviated RA clinical score and paw inflammation, balanced Th17 and Treg cells’ responses and population, modulated levels of pro-inflammatory and anti-inflammatory cytokines in joint synovial fluid, and inhibited FLS activation in CIA rats. Ber-BMSC-EXs (with a significantly greater effect than BMSC-EXs) could significantly ameliorate synovial joint inflammation and clinical symptoms of arthritis in CIA rats, showing that berberine can reinforce anti-arthritic effects of BMSC-EXs.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44607338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}