Food and Agricultural Immunology最新文献

筛选
英文 中文
Immunostimulatory activity of Paeonia lactiflora through TLR4-dependent activation of p38, JNK, and ERK1/2 in mouse macrophages, RAW264.7 cells 通过tlr4依赖性激活小鼠巨噬细胞、RAW264.7细胞中的p38、JNK和ERK1/2的免疫刺激活性
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2023-07-06 DOI: 10.1080/09540105.2023.2222930
Min Yeong Choi, So-Jung Park, J. Jeong
{"title":"Immunostimulatory activity of Paeonia lactiflora through TLR4-dependent activation of p38, JNK, and ERK1/2 in mouse macrophages, RAW264.7 cells","authors":"Min Yeong Choi, So-Jung Park, J. Jeong","doi":"10.1080/09540105.2023.2222930","DOIUrl":"https://doi.org/10.1080/09540105.2023.2222930","url":null,"abstract":"ABSTRACT Paeonia lactiflora (P. lactiflora) has been recently reported to increase the production of proinflammatory mediators and activates phagocytosis in macrophages. Thus, in this study, we tried to verify the macrophage activation of Paeoniae Radix Rubra (PRR, also known as red peony root) and elucidate its mechanism of action. PRR upregulated the production of proinflammatory mediators and activated phagocytosis in RAW264.7 cells. However, these effects were reversed by inhibition of TLR2/4. In addition, the inhibition of p38, JNK, and ERK1/2 reduced the PRR-mediated production of proinflammatory mediators, and the SPL-mediated activation of p38, JNK, and ERK1/2 was blocked by the TLR4 inhibition. These findings indicate that PRR may activate macrophages through TLR4-dependent activation of p38, JNK, and ERK1/2. These indicate that PRR has immunostimulatory activity. Thus, it is believed that PRR can be used as a functional food agent that enhances the immune system.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47260269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oral administration of vermicompost tea ameliorates eczema skin inflammation via regulation of Th2 immune response 口服粉丝茶通过调节Th2免疫反应改善湿疹皮肤炎症
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2023-07-06 DOI: 10.1080/09540105.2023.2223366
Huijiao Wen, Ci Lu, Runmen Hu, W. Shi, Libang Zhou, Puzhao Wen, Shan Jiang, Y. Lo
{"title":"Oral administration of vermicompost tea ameliorates eczema skin inflammation via regulation of Th2 immune response","authors":"Huijiao Wen, Ci Lu, Runmen Hu, W. Shi, Libang Zhou, Puzhao Wen, Shan Jiang, Y. Lo","doi":"10.1080/09540105.2023.2223366","DOIUrl":"https://doi.org/10.1080/09540105.2023.2223366","url":null,"abstract":"ABSTRACT Vermicompost tea (VCT) is the effluent or leachate with a honey-brown colour collected during vermicomposting, an ecologically significant process essential to today’s organic and regenerative agriculture. Dried earthworm, a.k.a. dilong (DL, meaning “earth dragon”) in traditional Chinese herbal medicine, has long been used as a key component to treat diverse skin diseases, including eczema. In the present study, we hypothesized that VCT might have a positive influence on eczema therapy. It was found that the oral application of 50% VCT in mouse model reduced the ear allergic scores and alleviated the histological changes caused by eczema. Furthermore, the levels of Th2-associated and pro-inflammatory cytokines (namely IL-4 and IL-13 in serum) and IgE (in serum and ear tissues) were significantly reduced by VCT. Therefore, oral administration of 50% VCT exerts immunomodulatory effects on the development of eczema, suggesting its potential as a nutraceutical candidate for eczema treatment. GRAPHICAL ABSTRACT","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42297877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Serotype and antimicrobial resistance of Salmonella from poultry meats in 2021 in Shanghai, China 2021年上海禽肉中沙门氏菌的血清型和耐药性
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2023-06-18 DOI: 10.1080/09540105.2023.2220568
Quan Xiao, Ying Yu, Biyao Xu, Zhixin Fang, Wenjie Chen, Jun Feng, Yanqi Zhu, Yue Liu, Qifang Gu, Jiayuan Luo, X. Song, Zengfeng Zhang, Min Chen, Hongzhi Zhang
{"title":"Serotype and antimicrobial resistance of Salmonella from poultry meats in 2021 in Shanghai, China","authors":"Quan Xiao, Ying Yu, Biyao Xu, Zhixin Fang, Wenjie Chen, Jun Feng, Yanqi Zhu, Yue Liu, Qifang Gu, Jiayuan Luo, X. Song, Zengfeng Zhang, Min Chen, Hongzhi Zhang","doi":"10.1080/09540105.2023.2220568","DOIUrl":"https://doi.org/10.1080/09540105.2023.2220568","url":null,"abstract":"ABSTRACT Salmonella is a major cause of food poisoning, and its infection and antimicrobial resistance vary regionally due to different sanitary standards and the use of antimicrobials. The aim of this study is to evaluate the microbiological contamination and antimicrobial resistance of Salmonella in Shanghai, providing references for supervision over food safety and clinical treatment. This study analysed the distribution of serotypes, the distribution of antimicrobial resistance in different poultry meats and serotype of Salmonella, the correlation between different antimicrobial resistance, and multiple drug resistance (MDR) of 139 strains of Salmonella from poultry meats. The results showed that the proportion of Salmonella detected in pigeons was high, which could cause severe diseases to humans. The most common resistance was observed to TET (about 60%), and resistance was even found to IPM. Resistance to two or more antimicrobials was accounted for 75.5%, indicating a wide spreading MDR.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47480889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a novel polyclonal antibody against bovine αS1-casein IgE epitopes for prediction of potential allergenicity of milk in foods 一种新的抗牛αS1酪蛋白IgE表位多克隆抗体的开发用于预测牛奶在食品中的潜在致敏性
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2023-06-18 DOI: 10.1080/09540105.2023.2222932
Shengfa He, Caiyun Long, Liming Li, Yan Yan, Jinyu Li, Meng Xiong, Jiangqiang Zhao, Ziling Feng, Yang Li, Yaping Dong, Xin Li, Hongbing Chen
{"title":"Development of a novel polyclonal antibody against bovine αS1-casein IgE epitopes for prediction of potential allergenicity of milk in foods","authors":"Shengfa He, Caiyun Long, Liming Li, Yan Yan, Jinyu Li, Meng Xiong, Jiangqiang Zhao, Ziling Feng, Yang Li, Yaping Dong, Xin Li, Hongbing Chen","doi":"10.1080/09540105.2023.2222932","DOIUrl":"https://doi.org/10.1080/09540105.2023.2222932","url":null,"abstract":"ABSTRACT Bovine αS1-casein (αS1-CN) is the main allergen in cow's milk, and immunoglobulin E (IgE) epitopes are the material basis to trigger allergic reactions. Thus, IgE epitopes could be as key targets for precise αS1-CN detection and allergenicity assessment. Here, we designed a tandem containing nine IgE linear epitopes of αS1-CN (tαS1-CN), expressed the recombinant tαS1-CN in Escherichia coli, and prepared the rabbit polyclonal antibody against recombinant tαS1-CN (pAb-tαS1-CN). The obtained recombinant tαS1-CN had a purity of 94.43% and retained the antigenicity and potential allergenicity of IgE epitopes. The pAb-tαS1-CN could recognize the nine IgE epitopes of αS1-CN, and also showed high specificity. The ability of pAb-tαS1-CN to evaluate the potential allergenicity of milk in foods was validated by sera from cow's milk allergy patients. These findings indicate that our prepared pAb-tαS1-CN may represents a valuable tool for the evaluation of milk allergenicity in processed foods.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43287676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of selected bioactive substances and nanoparticles on the immunoreactivity of edible packages containing chitosan, by the ELISA method 采用酶联免疫吸附试验(ELISA)研究了几种生物活性物质和纳米颗粒对壳聚糖食用包装免疫反应性的影响
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2023-06-18 DOI: 10.1080/09540105.2023.2222933
L. Havlová, M. Pospiech, Z. Javůrková, M. Bartlová, K. Těšíková, D. Dordević, S. Dordevic, J. Zemancová, B. Tremlová
{"title":"Effect of selected bioactive substances and nanoparticles on the immunoreactivity of edible packages containing chitosan, by the ELISA method","authors":"L. Havlová, M. Pospiech, Z. Javůrková, M. Bartlová, K. Těšíková, D. Dordević, S. Dordevic, J. Zemancová, B. Tremlová","doi":"10.1080/09540105.2023.2222933","DOIUrl":"https://doi.org/10.1080/09540105.2023.2222933","url":null,"abstract":"ABSTRACT The aim of this study was evaluated the effect of selected bioactive substances and nanoparticles on the immunoreactivity of edible packages based on chitosan using the ELISA. The analysed protein was the tropomyosin. The results confirmed the presence of the tropomyosin (3.77 ± 0.79–5.75 ± 0.01 µg/g) in control samples. This study demonstrated that the bioactive substances in the form of grape (0.61 ± 0.34–0.43 ± 0.16 µg/g), blueberry (0.58 ± 0.32–0.39 ± 0.27 µg/g), and parsley extracts (2.09 ± 1.28–0.79 ± 0.40 µg/g) reduces immunoreactivity (p < 0.05) of the tropomyosin. The elder pollen had no significant effect (p > 0.05) on immunoreactivity. ZnO and TiO2 nanoparticles also demonstrated immunoreactivity reduction (p < 0.05). The exception was silver nanoparticles, where the immunoreactivity increased with increasing concentration of grape extract (1.29 ± 0.01–5.47 ± 0.25 µg/g). The results confirmed the inhibitory effect of bioactive substances on the immunoreactivity of the used ELISA and also showed the need to consider immunoreactive substances when interpreting ELISA results.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46655133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcriptomics reveals the potential mechanism of ellagic acid extract from raspberry on wound healing 转录组学揭示了覆盆子鞣花酸提取物促进伤口愈合的潜在机制
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2023-06-09 DOI: 10.1080/09540105.2023.2214709
Yong-xiang Wu, Chan-Chan Liu, Wenjing Lu, Siting Li, Zhou Chen, Junxia Xia, Yiling Tian, Aijin Ma, Yingmin Jia
{"title":"Transcriptomics reveals the potential mechanism of ellagic acid extract from raspberry on wound healing","authors":"Yong-xiang Wu, Chan-Chan Liu, Wenjing Lu, Siting Li, Zhou Chen, Junxia Xia, Yiling Tian, Aijin Ma, Yingmin Jia","doi":"10.1080/09540105.2023.2214709","DOIUrl":"https://doi.org/10.1080/09540105.2023.2214709","url":null,"abstract":"ABSTRACT The aim of this study was to reveal the mechanism of its wound healing function at the molecular level using transcriptome sequencing technology. The results showed that EAE mainly upregulated Cyclin A gene through AMPK signalling pathway, activated cell cycle pathway, promoted DNA synthesis, accelerated mitosis, and thus promoted the proliferation of Human immortalized keratinocytes (HaCaT) cells. In addition, analysis of downregulated gene enrichment revealed that these genes were significantly or very significantly enriched not only in the cytokine-cytokine receptor interaction pathway but also in the TNF and the NF-κB signalling inflammatory classical pathway. It was mainly through downregulation of genes such as Fn14, CCL2, CCL5, CARD14 and LTB that prevented inflammatory responses while delaying apoptosis. The results of this study provided important information for the molecular mechanism of wound healing of EAE and provided a basis for further development of functional products of raspberry.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42994697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protective mechanisms of intra- and extracellular polysaccharides from Schizophyllum commune on H2O2-induced oxidative damage of human skin fibroblasts 裂藻胞内和胞外多糖对h2o2诱导的人皮肤成纤维细胞氧化损伤的保护机制
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2023-05-29 DOI: 10.1080/09540105.2023.2203407
Wenjing Cheng, Yundong Yang, Jiachan Zhang, Feiqian Di, Luyao Li, Changtao Wang, Meng Li, Dan Zhao, X. Shi, Tong Huo, Quan An
{"title":"Protective mechanisms of intra- and extracellular polysaccharides from Schizophyllum commune on H2O2-induced oxidative damage of human skin fibroblasts","authors":"Wenjing Cheng, Yundong Yang, Jiachan Zhang, Feiqian Di, Luyao Li, Changtao Wang, Meng Li, Dan Zhao, X. Shi, Tong Huo, Quan An","doi":"10.1080/09540105.2023.2203407","DOIUrl":"https://doi.org/10.1080/09540105.2023.2203407","url":null,"abstract":"ABSTRACT Schizophyllum commune is a fungus with applications both in medicine and foods, which produces IPS and EPS. However, there is no comparative study on the activity comparison of IPS and EPS and its mechanism of antioxidant effect. In this study, the antioxidant activities of the two polysaccharides were measured in biochemical, cellular and molecular levels, and H2O2 induced oxidative damage model of HSFs was established to explore the protective effects of EPS and IPS. We found IPS and EPS both achieved a good antioxidant effect. They can also improve the cellular levels of SOD and reduce MDA. Insights regarding the molecular mechanism were studied, we found that both EPS and IPS could increase the expression of Nrf2 in Keap1-Nrf2/ARE signaling pathway. At the same time, they could inhibit the expression of the negative feedback gene Keap1 and promote the expression of downstream gene GCLC, Gstt-1, Nqo1 and Ho-1.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41975382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Deep-sea-derived viridicatol relieves allergic response by suppressing MAPK and JAK-STAT signalling pathways of RBL-2H3 cells 深海源性vi讥讽醇通过抑制RBL-2H3细胞的MAPK和JAK-STAT信号通路减轻过敏反应
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2023-05-29 DOI: 10.1080/09540105.2023.2207791
Yan Liu, Zhendan Shu, Yan Li, Hui-xiong Chen, Hong Liu, Xianwen Yang, Guangming Liu, Qingmei Liu
{"title":"Deep-sea-derived viridicatol relieves allergic response by suppressing MAPK and JAK-STAT signalling pathways of RBL-2H3 cells","authors":"Yan Liu, Zhendan Shu, Yan Li, Hui-xiong Chen, Hong Liu, Xianwen Yang, Guangming Liu, Qingmei Liu","doi":"10.1080/09540105.2023.2207791","DOIUrl":"https://doi.org/10.1080/09540105.2023.2207791","url":null,"abstract":"ABSTRACT Our previous studies reported that viridicatol isolated from the deep-sea-derived fungus Penicillium griseofulvum could regulate the stabilisation of mast cells to relieve food allergy. To understand the molecular role of viridicatol in stabilising mast cells, transcriptomes of viridicatol-treated RBL-2H3 cells were analysed by RNA-sequencing. There were 128 differentially expressed genes in activated RBL-2H3 cells with or without viridicatol treatment. The mast cell activation-related genes were significantly reduced by treatment with viridicatol through RT-qPCR analysis. Moreover, Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that viridicatol was important in mast cell stabilisation by affecting MAPK and JAK-STAT signalling pathways. Additionally, molecular docking and western blot analysis revealed that the phosphorylated JNK, ERK, P38, and STAT6 proteins were inhibited by viridicatol. Taken together, viridicatol has the potential to be used as a new type of anti-food allergic functional material via controlling MAPK and JAK-STAT signalling pathways of mast cells.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41962549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Litopenaeus vannamei fermentation using selected Lactobacillus spp. to reduce its allergenicity 用选定的乳杆菌发酵凡纳滨对虾以降低其致敏性
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2023-05-29 DOI: 10.1080/09540105.2023.2210267
Hongyan Li, Zhihe Hu, Yali Yan
{"title":"Litopenaeus vannamei fermentation using selected Lactobacillus spp. to reduce its allergenicity","authors":"Hongyan Li, Zhihe Hu, Yali Yan","doi":"10.1080/09540105.2023.2210267","DOIUrl":"https://doi.org/10.1080/09540105.2023.2210267","url":null,"abstract":"ABSTRACT Shrimp is a common cause of food allergies in allergic individuals. The management of patients suffering from shrimp allergy relies on avoidance of the specific food, which compromises nutritional requirements. In this study, fermentation was performed using Lactobacillus to reduce the allergenicity of Litopenaeus vannamei. The optimal fermentation conditions using Lactobacillus helveticus TS6024 were 1% glucose, 30% minced shrimp, 2.5% inoculum level, 3% NaCl, and 60 h of incubation at 37°C, whereas those using Lactobacillus acidophilus 6005 were 2% glucose, 20% minced shrimp, 15% inoculum level, 2% NaCl, and 48 h of incubation at 42°C. Under optimal conditions, Lactobacillus helveticus TS6024 and Lactobacillus acidophilus 6005 fermentation reduced the allergenicity of shrimp by 78.97% and 70.09%, respectively. SDS-PAGE analysis showed that the band intensity of tropomyosin (36KDa) reduced evidently after the selected Lactobacillus fermentation. The hypoallergenic shrimp/shrimp products will provide optimal nutrition with reduced risk of adverse reactions in hypersensitive individuals.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49426738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Simultaneous detection of OTA and AFB1 in cereals based on bispecific monoclonal antibody using quantum dot nanobead lateral flow immunoassay 基于双特异性单克隆抗体的量子点纳米球横向流动免疫法同时检测谷物中OTA和AFB1
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2023-05-09 DOI: 10.1080/09540105.2023.2206596
Mengyun Wu, Jun Xia, Tingting Liu, Guangjian Xue, Xuechen Fang, W. Lai, Juan Peng
{"title":"Simultaneous detection of OTA and AFB1 in cereals based on bispecific monoclonal antibody using quantum dot nanobead lateral flow immunoassay","authors":"Mengyun Wu, Jun Xia, Tingting Liu, Guangjian Xue, Xuechen Fang, W. Lai, Juan Peng","doi":"10.1080/09540105.2023.2206596","DOIUrl":"https://doi.org/10.1080/09540105.2023.2206596","url":null,"abstract":"ABSTRACT Simultaneous contamination of ochratoxin A (OTA) and aflatoxin B1 (AFB1) in cereals are an ongoing global concern. For this reason, a sensitive method for the simultaneous detection of OTA and AFB1 in soybean, corn, and rice is required. Herein, we prepared a bispecific monoclonal antibody (BsMAb) that specifically recognized OTA and AFB1, and subsequently developed a quantum dot nanobead (QB) lateral flow immunoassay (QB-LFIA) based on BsMAb. The half-inhibitory concentrations (IC50) for OTA and AFB1 detection were 0.73 and 0.01 µg/kg in soybean, 1.14 and 0.03 µg/kg in corn, and 1.69 and 0.07 µg/kg in rice, respectively. The average recoveries of OTA and AFB1 were 80.20–130.05% with coefficients of variation below 11%. The performance of the proposed method was compared with that of traditional LFIA, and higher sensitivity was observed. The BsMAb-based QB-LFIA was sensitive and specific for the simultaneous detection of OTA and AFB1 in cereals.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47062776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信
小红书