Fish Physiology and Biochemistry最新文献

{"title":"Characterization of sexual size dimorphism in mandarin fish and association with igfbp-5a/b regulation","authors":"Xiaoli Yao, Yunhong Zhou, Kenneth Nyirenda, Yindu Song, Chenxi Ma, Kelin Qian, Yan Zhao, Shoujie Tang, Jinliang Zhao","doi":"10.1007/s10695-024-01385-8","DOIUrl":"https://doi.org/10.1007/s10695-024-01385-8","url":null,"abstract":"<p>Mandarin fish (<i>Siniperca chuatsi</i>) is an important cultured fish in East Asia that shows sexual size dimorphism (SSD), with females growing faster than males when reaching marketable size. However, the regulatory mechanism of SSD is not clear. To characterize SSD of mandarin fish and its association with <i>gh</i>/<i>igf1</i>/<i>igfbp-5</i> expression, gonadal developmental atlas of the females and the males were described, and growth parameters and serum levels of E2 and T, as well as the relative expression levels of <i>gh</i>, <i>igf1</i>, and <i>igfbp-5a/b</i> mRNAs, were determined. The results showed that the logistic growth equation of body mass and total length of female and male were W(♀) = 667.57/(1 + e^(4.19 − 1.24*t)), W(♂) = 582.71/(1 + e^(4.07 − 1.27*t)), L(♀) = 31.47/(1 + e^1.95 − 1.08*t)), L(♂) = 26.20/(1 + e^(2.56 − 1.5*t)). The month of inflection points for body mass for females and males were 3.37 mph and 3.20 mph, respectively, when the body mass were 333.79 g and 291.36 g. The month of inflection points for total length growth were 1.80 mph and 1.70 mph, respectively, when the total length were 18.52 cm and 16.28 cm. At 1.5–2.0 mph, SSD was not clearly demonstrated. At 3.0 mph, the body mass of the females was significantly higher than that of the males (<i>P</i> &lt; 0.05), Serum E2, brain <i>gh</i>, and liver <i>igf1</i> expression of the females was significantly higher than that of the males (<i>P</i> &lt; 0.05); T content of the males was significantly higher than that of the females (<i>P</i> &lt; 0.05). At 4.0 months of age, the body mass of the females was highly significantly higher than that of the males (<i>P</i> &lt; 0.01), Serum E2, brain <i>gh</i>, and liver <i>igf1</i> expression of the females was highly significantly higher than that of the males (<i>P</i> &lt; 0.05); T content of the males was significantly higher than that of the females (<i>P</i> &lt; 0.05). With the continuous development of gonads, muscle and liver <i>igfbp-5a</i> and <i>-5b</i> expression generally tend to increase in females and males, while <i>igfbp-5a</i> showed a gradual increasing trend, and <i>igfbp-5b</i> expression showed a trend of decreasing and then increasing. Male <i>igfbp-5a/b</i> expression was significantly higher than female at the age of 3.0–4.0 months (<i>P</i> &lt; 0.05). This work verified that the females had faster growth rate since 3.0 mph compared to the males, which may be related to higher E2 levels in females leading to higher <i>igf1</i> level, through inhibition of <i>igfbp-5a/b</i> expression.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"216 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141882858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A biofloc system avoids the adverse effects of diets with suboptimal protein levels on zootechnical performance, intestinal histomorphometry, and protein metabolism of Nile tilapia juvenile fed Spirulina biomass (Arthrospira platensis) as an alternative protein source.","authors":"Fernanda Picoli, Alana D de Oliveira, Suelyn O Marques, Deise C Terhorst, Suélen Serafini, Luísa Nora, Fabio F Neves, Maurício G C Emerenciano, Diogo L A Lopes, Aleksandro S da Silva, Thiago E H P Fabregat","doi":"10.1007/s10695-024-01358-x","DOIUrl":"10.1007/s10695-024-01358-x","url":null,"abstract":"<p><p>This study aimed to evaluate the effect of the biofloc technology (BFT) system and the replacement of fish meal with Spirulina biomass on productive performance, intestinal histomorphometry, plasma biochemistry, and oxidative stress of Nile tilapia juveniles (Oreochromis niloticus) fed suboptimal levels of protein. Two factors were evaluated: production systems (clear water × BFT) and replacement of fish meal with Spirulina (0, 33, 66 e 100%). The design was in a 2 × 4 randomized factorial scheme with four replications, and the fish were evaluated for 48 days. Four isoproteic (28% crude protein) diets were formulated with gross energy values close to 4300 kcal kg^-1. Nile tilapia juveniles (0.23 ± 0.01 g) were distributed in 16 circular tanks (70 L) at seven fish/tank. The diets were formulated with protein levels approximately 20% below that required for the species and life stage. No interaction was observed between the factors evaluated (production systems × Spirulina inclusion). Rearing the fish in the BFT system avoided the adverse effects of diets with suboptimal protein levels on performance, intestinal histomorphometry, and protein metabolism. Lower values lower lipid peroxidation and higher antioxidant capacity were observed in fish reared in the BFT system, showing evidence of improvements in antioxidant responses and lower levels of physiological oxidative stress. Spirulina completely replaced fish meal in the diets of Nile tilapia juveniles without adverse effects on intestinal morphometry, protein metabolism, and antioxidant response. Replacing 66% of fish meal with Spirulina improved the productive performance, regardless of the rearing system.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":" ","pages":"1605-1620"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EPA and DHA promote cell proliferation and enhance activity of the Akt-TOR-S6K anabolic signaling pathway in primary muscle cells of turbot (Scophthalmus maximus L.).","authors":"Ya Gao, Chengdong Liu, Xuan Wang, Huihui Zhou, Kangsen Mai, Gen He","doi":"10.1007/s10695-024-01351-4","DOIUrl":"10.1007/s10695-024-01351-4","url":null,"abstract":"<p><p>Fish growth and health are predominantly governed by dietary nutrient supply. Although the beneficial effects of omega-3 polyunsaturated fatty acids supplementation have been shown in a number of fish species, the underlying mechanisms are still mostly unknown. In this study, we conducted an investigation into the effects of EPA and DHA on cell proliferation, nutrient sensing signaling, and branched-chain amino acids (BCAA) transporting in primary turbot muscle cells. The findings revealed that EPA and DHA could stimulate cell proliferation, promote protein synthesis and inhibit protein degradation through activation of target of rapamycin (TOR) signaling pathway, a pivotal nutrient-sensing signaling cascade. While downregulating the expression of myogenin and myostatin, EPA and DHA increased the level of myogenic regulatory factors, such as myoD and follistatin. Furthermore, we observed a significant increase in the concentrations of intracellular BCAAs following treatment with EPA or DHA, accompanied by an upregulation of the associated amino acid transporters. Our study providing valuable insights into the mechanisms underlying the growth-promoting effects of omega-3 fatty acids in fish.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":" ","pages":"1483-1494"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of toxic effects of ammonia on growth, pathology of liver and kidney tissues and relative expression of GH and IGF-1 Genes by CoQ₁₀ Supplementation in Oncorhynchus mykiss.","authors":"Ashkan Zargari, Mohammad Mazandarani, Roghieh Safari, Hossein Hoseinifar, Aliakbar Hedayati","doi":"10.1007/s10695-024-01365-y","DOIUrl":"10.1007/s10695-024-01365-y","url":null,"abstract":"<p><p>Reducing the negative impact of environmental and stressful factors is a crucial step in achieving sustainable aquaculture. Therefore, a study was aimed at evaluating the impacts of Coenzyme Q₁₀ (CoQ₁₀) supplementation on growth, relative gene expression of Growth Hormone (GH) and Insulin-like growth factor-1 (IGF-1), liver and kidney histopathology against stress induced by ammonia in Rainbow trout (Oncorhynchus mykiss). The fish were given feed containing different levels of CoQ₁₀ for 8 weeks: Control - CoQ₁₀ 0%, G1 - CoQ₁₀ 0.1%, G2 - CoQ₁₀ 0.5% and G3 - CoQ₁₀ 1%. At the end of the experiment, fish were exposed to ammonia stress concentration at 0.6mg/L for 24 h to assess liver and kidney tissue damage. Results showed that there was a significant activity increase in GH and IGF-1 genes due to supplementation with CoQ₁₀ alone (p < 0.05). Gene expression for GH increased about two-fold whereas that for IGF-1 experienced a four-fold upregulation compared to controls (p < 0.05). CoQ₁₀'s-related antioxidant effects probably minimized liver and kidney cellular injuries, as significant decreases were observed in ammonia-induced mortality (p < 0.05). In summary, adding CoQ₁₀ to the diet is a potential way to improve fish production through controlling the gene expression of GH and IGF-1, as well as making fish populations more resistant to possible future stress caused by ammonia in intensive or super-intensive aquaculture systems.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":" ","pages":"1711-1729"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141327408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Behavioral and electrophysiological study in Colossoma macropomum treated with different concentrations of Nepeta cataria oil in an immersion bath revealed a therapeutic window for anesthesia.","authors":"Murilo Farias Dos Santos, Lorena Meirelis do Nascimento, Clarissa Araújo da Paz, Tays Mata Câmara, Yan Kenzo Monteiro Motomya, Rayllan da Cunha Ferreira, Yris da Silva Deiga, Erika Monteiro, Sildiane Martins Cantanhêde, Lílian Lund Amado, Moisés Hamoy","doi":"10.1007/s10695-024-01361-2","DOIUrl":"10.1007/s10695-024-01361-2","url":null,"abstract":"<p><p>The purpose of this study was to characterize the activity of essential oils from Nepeta Cataria (EON) at concentrations of 125 <math><mrow><mi>μ</mi> <mtext>L</mtext></mrow> </math>  L-1, 150 <math><mrow><mi>μ</mi> <mtext>L</mtext></mrow> </math>  L-1, 175 <math><mrow><mi>μ</mi> <mtext>L</mtext></mrow> </math>  L-1, and 200 <math><mrow><mi>μ</mi> <mtext>L</mtext></mrow> </math>  L-1 on the behavior of loss of the posture reflex and recovery of the posture reflex and electrocardiographic activity and recording of the opercular beat of Colossoma macropomum during immersion bathing for a period of 5 min, in order to obtain a window for safe use during anesthesia. The fish (23.38 ± 3.5 g) were assigned to the following experiments: experiment 1 (latency to loss and recovery of the posture reflex): (a) 125 <math><mrow><mi>μ</mi> <mtext>L</mtext></mrow> </math>  L-1, (b) 150 <math><mrow><mi>μ</mi> <mtext>L</mtext></mrow> </math>  L-1, (c) 175 <math><mrow><mi>μ</mi> <mtext>L</mtext></mrow> </math>  L-1, and (d) 200 <math><mrow><mi>μ</mi> <mtext>L</mtext></mrow> </math>  L-1 (n = 9) per group. Experiment 2 (electrocardiographic and heartbeat recordings): (a) control group; (b) vehicle control group (2 ml of alcohol per liter of water), (c) 125 <math><mrow><mi>μ</mi> <mtext>L</mtext></mrow> </math>  L-1, (d) 150 <math><mrow><mi>μ</mi> <mtext>L</mtext></mrow> </math>  L-1, (e) 175 <math><mrow><mi>μ</mi> <mtext>L</mtext></mrow> </math>  L-1, and (f) 200 <math><mrow><mi>μ</mi> <mtext>L</mtext></mrow> </math>  L-1 (n = 9), per group. All the concentrations used showed efficacy in inducing loss of the posture reflex and reversibility with recovery of the posture reflex, but the electrocardiographic recordings indicated morphographic changes such as bradycardia during induction and p wave apiculation during recovery at the highest concentrations tested. In this way, we suggest a safe use window for short-term anesthesia with EON in the concentration range of 125 to 150 <math><mrow><mi>μ</mi> <mtext>L</mtext></mrow> </math>  L-1 for juvenile Colossoma macropomum.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":" ","pages":"1651-1665"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of bile salts on intestinal epithelial function in gilthead seabream (Sparus aurata).","authors":"J Fuentes, S F Gregório, F Fonseca, R Robles-Arozarena, J A Martos-Sitcha, F J Moyano","doi":"10.1007/s10695-024-01369-8","DOIUrl":"10.1007/s10695-024-01369-8","url":null,"abstract":"<p><p>In the context of modern aquaculture, the effort to reduce the reliance on fishmeal/marine ingredients in fish diets has led to the exploration of plant-based protein sources as potential substitutes, a dietary shift that disrupts the bile acid profile in fish. Therefore, bile salts are being sought as additives. However, artificially increased intestinal levels of bile acids may significantly impact mucosal function. Therefore, here, we explored the regulatory role in the intestine of gilthead sea bream (Sparus aurata) of (i) chenodeoxycholic acid (CDC), (ii) a mixture formed by two bile acids, 3% cholic acid and 97% deoxycholic acid (MIX), and (iii) a conjugated bile salt sodium taurocholate (TC) in Ussing chambers with the epithelial voltage clamp technique. We tested the bile salts in a 50-500 μg/ml concentration range, and all of them promoted ion absorption. Yet, clear concentration-dependent and more pronounced effects on the ion transport were observed in the posterior intestine. On the other hand, bile salts had no or minor effects on tissue resistance. However, there are indications that the MIX could have adverse effects at high concentrations (500 μg/ml), promoting a threefold increase in tissue permeability measured using FITC-dextran (4 kD) regardless of the intestinal region, thus suggesting an alteration in intestinal permeability at high bile salt concentrations. The findings from our study emphasize the importance of considering intestinal function when contemplating the possible use of a particular bile salt as a dietary supplement. It appears that bile salts, whether acting individually or in combination, play a pivotal role in orchestrating nutrient absorption by influencing the function of epithelial ion transport. However further research is needed to fully grasp the region-dependent nuances of bile salt effects on ion transport and the ultimate consequences for nutrient absorption in the context of fish aquaculture.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":" ","pages":"1777-1790"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11286703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141445924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin-like growth factor binding protein-3 (igfbp-3) and igfbp-5 in yellowtail kingfish (Seriola lalandi): molecular characterization and expression levels under different nutritional status and stocking density.","authors":"Jichang Zheng, Wenjing Zhang, Yongjiang Xu, Aijun Cui, Yan Jiang, Bin Wang","doi":"10.1007/s10695-024-01359-w","DOIUrl":"10.1007/s10695-024-01359-w","url":null,"abstract":"<p><p>Insulin-like growth factor-binding proteins (IGFBPs) play important roles in regulating growth and development by binding to IGF, where IGFBP-3 and IGFBP-5 are the main binding carriers of IGF in the circulation system. In the present study, the gene sequences of igfbp-3, igfbp-5a, and igfbp-5b were cloned from the liver of yellowtail kingfish (Seriola lalandi). The ORF sequences of igfbp-3, igfbp-5a, and igfbp-5b were 888, 801, and 804 bp in length, which encoded 295, 266, and 267 amino acids, respectively. The above three genes were widely expressed in yellowtail kingfish tissues, with igfbp-3 being the most highly expressed in the heart, brain, and gonads, while igfbp-5a and igfbp-5b were both most highly expressed in the liver and kidney. The expression levels of igfbp-3, igfbp-5a, and igfbp-5b were detected throughout the embryonic and larval stages, suggesting their roles in early development and growth regulation of yellowtail kingfish. Besides, igfbp-3 and igfbp-5a were significantly up-regulated in the liver under food deprivation and high-density rearing conditions, which was exactly opposite to the growth performance of yellowtail kingfish, implying that they may serve as biomarkers of adverse culture conditions. Overall, the above results initially identified the molecular characteristics of igfbp-3/-5a/-5b in yellowtail kingfish and implied that they might play important roles in the growth and development, providing a basis for further research on underlying regulatory mechanisms.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":" ","pages":"1621-1633"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of large yellow croaker (Larimichthys crocea) at different growth rates.","authors":"Yang Gao, Xuming Huang, Yanli Liu, Huirong Lv, Xiaolong Yin, Weiye Li, Zhangjie Chu","doi":"10.1007/s10695-024-01367-w","DOIUrl":"10.1007/s10695-024-01367-w","url":null,"abstract":"<p><p>The unsynchronized growth of the large yellow croaker (Larimichthys crocea), which impacts growth efficiency, poses a challenge for aquaculture practitioners. In our study, juvenile stocks of large yellow croaker were sorted by size after being cultured in offshore cages for 4 months. Subsequently, individuals from both the fast-growing (FG) and slow-growing (SG) groups were sampled for analysis. High-throughput RNA-Seq was employed to identify genes and pathways that are differentially expressed during varying growth rates, which could suggest potential physiological mechanisms that influence growth rate. Our transcriptome analysis identified 382 differentially expressed genes (DEGs), comprising 145 upregulated and 237 downregulated genes in comparison to the SG group. GO and KEGG enrichment analyses indicated that these DEGs are predominantly involved in signal transduction and biochemical metabolic pathways. Quantitative PCR (qPCR) results demonstrated that cat, fasn, idh1, pgd, fgf19, igf2, and fads2 exhibited higher expression levels, whereas gadd45b and gadd45g showed lower expression compared to the slow-growing group. In conclusion, the differential growth rates of large yellow croaker are intricately associated with cellular proliferation, metabolic rates of the organism, and immune regulation. These findings offer novel insights into the molecular mechanisms and regulatory aspects of growth in large yellow croaker and enhance our understanding of growth-related genes.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":" ","pages":"1745-1757"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary and functional analysis of metabotropic glutamate receptors in lampreys.","authors":"Ruyu Zhuang, Zihao Yan, Yicheng Gao, Ayqeqan Nurmamat, Shuyuan Zhang, Min Xiu, Yuesi Zhou, Ya Pang, Ding Li, Liang Zhao, Xin Liu, Yinglun Han","doi":"10.1007/s10695-024-01374-x","DOIUrl":"10.1007/s10695-024-01374-x","url":null,"abstract":"<p><p>The metabotropic glutamate receptor (mGluR, GRM) family is involved in multiple signaling pathways and regulates neurotransmitter release. However, the evolutionary history, distribution, and function of the mGluRs family in lampreys have not been determined. Therefore, we identified the mGluRs gene family in the genome of Lethenteron reissneri, which has been conserved throughout vertebrate evolution. We confirmed that Lr-GRM3, Lr-GRM5, and Lr-GRM7 encode three types of mGluRs in lamprey. Additionally, we investigated the distribution of Lr-GRM3 within this species by qPCR and Western blotting. Furthermore, we conducted RNA sequencing to investigate the molecular function of Lr-GRM3 in lamprey. Our gene expression profile revealed that, similar to that in jawed vertebrates, Lr-GRM3 participates in multiple signal transduction pathways and influences synaptic excitability in lampreys. Moreover, it also affects intestinal motility and the inflammatory response in lampreys. This study not only enhances the understanding of mGluRs' gene evolution but also highlights the conservation of GRM3's role in signal transduction while expanding our knowledge of its functions specifically within lampreys. In summary, our experimental findings provide valuable insights for studying both the evolution and functionality of the mGluRs family.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":" ","pages":"1861-1877"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early stress exposure on zebrafish development: effects on survival, malformations and molecular alterations.","authors":"David G Valcarce, Alba Sellés-Egea, Marta F Riesco, María-Gracia De Garnica, Beatriz Martínez-Fernández, María Paz Herráez, Vanesa Robles","doi":"10.1007/s10695-024-01355-0","DOIUrl":"10.1007/s10695-024-01355-0","url":null,"abstract":"<p><p>The effects of stress during early vertebrate development can be especially harmful. Avoiding stressors in fish larvae is essential to ensure the health of adult fish and their reproductive performance and overall production. We examined the consequences of direct exposure to successive acute stressors during early development, including their effects on miR-29a and its targets, survival, hatching and malformation rates, larval behaviour and cartilage and eye development. Our aim was to shed light on the pleiotropic effects of early-induced stress in this vertebrate model species. Our results showed that direct exposure to successive acute stressors during early development significantly upregulated miR-29a and downregulated essential collagen transcripts col2a1a, col6a2 and col11a1a, decreased survival and increased malformation rates (swim bladder, otoliths, cardiac oedema and ocular malformations), promoting higher rates of immobility in larvae. Our results revealed that stress in early stages can induce different eye tissular architecture and cranioencephalic cartilage development alterations. Our research contributes to the understanding of the impact of stressful conditions during the early stages of zebrafish development, serving as a valuable model for vertebrate research. This holds paramount significance in the fields of developmental biology and aquaculture and also highlights miR-29a as a potential molecular marker for assessing novel larval rearing programmes in teleost species.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":" ","pages":"1545-1562"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11286684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}