大西洋狼鱼(Anarhichas lupus)的水产养殖潜力:应激和对急性处理的生理反应。

IF 2.5 3区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ida Hedén, Jonathan Armand Charles Roques, Marica Andersson, Niklas Warwas, Raneesha de Fonseka, Darragh Doyle, James Hinchcliffe, Elisabeth Jönsson, Kristina Sundell, Henrik Sundh
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引用次数: 0

摘要

大西洋狼鱼(Anarhichas lupus)是一种冷水鱼,具有使北方国家水产养殖多样化的潜力。很少有研究对大西洋狼鱼的应激生理进行调查,目前对狼鱼物种的应激知识主要来自近亲斑点狼(Anarhichas minor)。在目前的研究中,大西洋狼鱼被暴露在处理压力中,模仿水产养殖中常见的饲养条件,如反复暴露在空气中和追网。在应力暴露之前采集样本(预应力;对照)以及应激后5和24小时。评估一系列原发性和继发急性应激反应参数:血浆皮质醇、葡萄糖和乳酸水平,血液学指标(血红蛋白、Hb;比容、Hct;平均红细胞血红蛋白浓度(MCHC),以及通过血浆渗透压和鳃Na+/K+ atp酶(NKA)活性的渗透调节能力。其他影响鱼类健康和福利的次生应激反应是肠道完整性和运输功能。这些参数是用乌辛室技术评估的。与其他鱼类相比,大西洋狼鱼在急性应激后的皮质醇峰值较低,并且在应激后24小时才出现,这表明大西洋狼鱼是一种缓慢而低的皮质醇反应。应激后5 ~ 24 h,血浆葡萄糖保持稳定,乳酸浓度显著下降。对pH、Hb或Hct没有影响,尽管在5 h和24 h后发现MCHC显著增加,这是由于Hb的小幅增加。这一结果表明应激暴露后Hb合成略有增加。应激后的肠道完整性、运输功能和鳃nka活性基本保持不变。总之,大西洋狼鱼似乎表现出相对温和的应激反应,其特点是缓慢而低的初级应激反应和最小的丈夫相关急性应激后的二次效应。这些发现有助于了解该物种作为海洋冷水养殖候选物种的发展潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Aquaculture potential of Atlantic wolffish (Anarhichas lupus): stress and physiological responses to acute handling.

The Atlantic wolffish (Anarhichas lupus) is a cold-water species with the potential to diversify aquaculture in Northern countries. Few studies have investigated the stress physiology of Atlantic wolffish, and the current knowledge on stress in wolffish species is largely derived from the closely related spotted wolffish (Anarhichas minor). In the current study, Atlantic wolffish were exposed to handling stress mimicking common husbandry conditions in aquaculture such as repeated air exposure and net-chasing. Samples were taken prior to stress exposure (pre-stress; control) as well as 5- and 24-h post-stress. A series of primary and secondary acute stress response parameters were assessed: plasma cortisol, glucose and lactate levels, hematological indices (hemoglobin, Hb; hematocrit, Hct; mean corpuscular hemoglobin concentration, MCHC), and osmoregulatory capacity through plasma osmolality and gill Na+/K+ATPase (NKA) activity. Other secondary stress responses with implications for fish health and welfare are intestinal integrity and transport functions. These parameters were assessed using the Ussing chamber technique. The cortisol peak values were low in comparison to other fish species studied after acute handling stress and occurred as late as 24 h post-stress, suggesting that Atlantic wolffish is a slow and low cortisol responder. Plasma glucose remained stable, whereas lactate concentrations significantly decreased between 5 and 24 h after stress. There was no effect on pH, Hb, or Hct, although a significant increase in MCHC was found after 5 h and 24 h, originating from a small increase in Hb. This result suggests a minor increase in Hb synthesis after stress exposure. The intestinal integrity and transport functions as well as gill NKA-activity remained unchanged after stress exposure. In conclusion, Atlantic wolffish appears to exhibit a relatively moderate stress response, characterized by a slow and low primary stress response and minimal secondary effects following husbandry-related acute stress. These findings contribute to the understanding of the species' potential for development as a candidate for marine, cold-water aquaculture.

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来源期刊
Fish Physiology and Biochemistry
Fish Physiology and Biochemistry 农林科学-生化与分子生物学
CiteScore
5.60
自引率
6.90%
发文量
106
审稿时长
4 months
期刊介绍: Fish Physiology and Biochemistry is an international journal publishing original research papers in all aspects of the physiology and biochemistry of fishes. Coverage includes experimental work in such topics as biochemistry of organisms, organs, tissues and cells; structure of organs, tissues, cells and organelles related to their function; nutritional, osmotic, ionic, respiratory and excretory homeostasis; nerve and muscle physiology; endocrinology; reproductive physiology; energetics; biochemical and physiological effects of toxicants; molecular biology and biotechnology and more.
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