Fish Physiology and Biochemistry最新文献

{"title":"Effect of dietary supplementation of lauric acid on growth performance, digestive enzymes, serum immune and antioxidant parameters, and intestinal morphology in black sea bream.","authors":"Sami Ullah, Fengqin Feng, Minjie Zhao, Jinzhi Zhang, Qingjun Shao","doi":"10.1007/s10695-025-01457-3","DOIUrl":"https://doi.org/10.1007/s10695-025-01457-3","url":null,"abstract":"<p><p>An eight-week feeding trial was conducted to examine the impact of dietary supplementation with lauric acid (LA) on juvenile black sea bream. A basal diet was formulated containing 19.9% fish meal, while five additional diets were prepared, each supplemented with varying levels of LA: LA1 (0.01%), LA2 (0.02%), LA3 (0.04%), LA4 (0.08%), and LA5 (0.16%), denoted as LA1 through LA5, respectively. Triplicate tanks were randomly allocated to each diet, each containing 20 fish with an initial weight of 1.55 ± 0.02 g. At the conclusion of the trial, the LA3 group exhibited significantly greater final body weight (FBW), weight gain (WG), specific growth rate (SGR), and protein efficiency ratio (PER) compared to the other groups (P < 0.05), while the feed conversion ratio (FCR) was markedly higher in the control group. No significant differences were detected among the groups in terms of initial body weight (IBW), muscle fiber index (MFI), protein productive value (PPV), condition factor (CF), hepatosomatic index (HSI), intraperitoneal fat (IPF), viscerosomatic index (VSI), and survival rate (SR) (P > 0.05). No significant variations were observed among the groups in the proximate compositions of the dorsal muscle and whole body (p > 0.05). Furthermore, no significant differences were observed in serum immune and antioxidant parameters in the midgut and hindgut and digestive enzyme activity (P > 0.05) among the treatment groups. However, the LA3 group demonstrated significantly higher levels of serum immune response markers IgM, C3, and C4 compared to the other groups, while malondialdehyde (MDA) levels were significantly elevated in the control group relative to the others. The LA3 group demonstrated significantly increased fore-intestinal villus height, crypt depth, villus height-to-crypt depth ratio, and goblet cell count per villus compared to the other groups (P < 0.05).</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"43"},"PeriodicalIF":2.5,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreserved sperm does not affect larval ontogeny and quality in Rhamdia quelen.","authors":"Vanessa Conceição Coimbra, Jeane Rodrigues, Raquel Santos Dos Santos, Rômulo Batista Rodrigues, Danilo Streit-Jr, Ana Luiza de Souza Caldas, Eduardo Silva do Nascimento Albuquerque, Evagno Junior da Silva Ferreira, Caio Maximino, Diógenes Henrique de Siqueira-Silva","doi":"10.1007/s10695-025-01455-5","DOIUrl":"https://doi.org/10.1007/s10695-025-01455-5","url":null,"abstract":"<p><p>Fish sperm cryopreservation is an important technique for optimizing juvenile production in aquaculture stations and laboratories and contributing to the conservation of endangered species. Despite its benefits, the cryopreservation process can cause cellular damage, affecting spermatozoa quality and offspring viability. This study aimed to evaluate the larval development of jundiá Rhamdia quelen originating from cryopreserved sperm. Larvae were obtained from artificial reproduction using oocyte samples from four females combined with fresh (Control) or cryopreserved/thawed sperm. The semen was diluted in the cryoprotective solution (1:3 ratio) consisting of skimmed milk powder (5%), methanol (10%), and fructose (5%), and was packaged into 0.25 mL straws. The straws were then stored and cooled in liquid nitrogen vapor for 18 h. The straws were individually warmed in a water bath at 25 °C for 10 s to thaw the samples. The experiments were performed in triplicates. Sperm quality, fertilization, hatching, and larval development were evaluated. After larval hatching, six larval collections were performed (5, 10, 15, 20, and 25 days after hatching), and 15 larvae were sampled per collection per treatment. Cryopreservation reduced sperm motility (70.48 ± 7.70 fresh to 41.36 ± 4.80 cryopreserved semen), progressivity (3874 fresh to 2505 cryopreserved semen), and beat cross frequency (55.83 ± 155 fresh to 50.22 ± 190 cryopreserved semen). Increased the percentage of sperm with abnormal morphology and increased most sperm pathologies. Furthermore, the fertilization rate was lower in the cryopreserved group (63.1 ± 18, and 83.72 ± 7.59 for fresh semen), while hatching was not different between groups (65.3 ± 18.05 fresh, 48.89 ± 21.77 cryopreserved semen) Otherwise, the initial larval development morphology showed no difference in the appearance of structures such as the presence of the vitelline structure, pigmentation pattern, development of the anal pore, embryonic membrane, eye, barbells, notochord flexion, and fin rays, for both treatments. There was no significant difference in the frequency of structures between larvae from fresh and cryopreserved/thawed sperm, revealing a similar developmental pattern in both treatments. In conclusion, the cryopreservation protocol affects sperm quality; however, those sperm able to fertilize the oocytes originate normal larvae with regular larval development of R. quelen up to 25 days old.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"42"},"PeriodicalIF":2.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary L-carnitine supplementation recovers the hepatic damage induced by high-fat diet in Nile tilapia (Oreochromis niloticus L.) via activation of Nrf2/Keap pathway and inhibition of pro-inflammatory cytokine.","authors":"Doaa H Assar, Abdallah S Salah, Aya G Rashwan, Ibrahim I Al-Hawary, Basma M Hendam, Ahmed Elsheshtawy, Amer Al Ali, Humood Al Shmrany, Zizy I Elbialy","doi":"10.1007/s10695-024-01430-6","DOIUrl":"https://doi.org/10.1007/s10695-024-01430-6","url":null,"abstract":"<p><p>A feeding trial for 8 weeks was performed to explore whether nutritional supplementation of L-carnitine may minimize the adverse effects of high-fat diet (HFD) on tilapia growth performance, antioxidant, immune parameters, inflammatory response, histopathology of liver, kidney, and intestine, as well as hepatic lipid metabolism aiming to reveal the mechanism and providing a shred of molecular evidence in Nile tilapia (Oreochromis niloticous). Six groups of the Nile tilapia (17.13 <math><mo>±</mo></math> 0.49 g) in triplicate were fed for 60 days. Six experimental diets were formulated, incorporating different concentrations of L-carnitine. The first three groups were administered a diet comprising 6% fat, with L-carnitine concentrations of 0, 0.5, and 1 g/kg diet was designated as F6Car0, F6Car0.5, and F6Car1, respectively. Moreover, the fourth, fifth, and sixth groups were fed on a diet containing 12% fat, with L-carnitine concentrations of 0, 0.5, and 1 g/kg diet, respectively termed F12Car0, F12Car0.5, and F12Car1. The main results were as follows: compared to the control group HFD caused a significant reduction in BWG and PER (P > 0.05), but significantly increased the feed conversion rate (FCR), hepatosomatic index (HSI), intraperitoneal fat (IPF), as well as increased visceral fat deposits and liver fat accumulation with higher activities of serum aminotransferases, glucose, triglycerides, and cholesterol. HFD exacerbates hepatic lipid accumulation by enhancing lipogenic gene expression. HFD-fed fish exhibited the lowest crude protein and highest crude fat levels. This study demonstrates that dietary supplementation with L-carnitine significantly boosts growth, improves hemato-biochemical parameters, decreases lipogenesis, elevates lipolysis pathway genes, and lowers lipid levels, thereby rebalancing lipid metabolism and lessening hepatic steatosis. It also mitigates inflammation by downregulating pro-inflammatory genes, upregulating immune genes, and positively affecting Nile tilapia's histopathology.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"40"},"PeriodicalIF":2.5,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary organic acid blend modulates hemato-immunological parameters, digestive and reproductive performances in red tilapia (Oreochromis niloticus × O. mossambicus) broodstock.","authors":"Abdel-Fattah M El-Sayed, El-Sayed Hemdan Eissa, Basma M Hendam, Hagar Sedeek Dighiesh, Heba E Abd Elnabi, Yasmin M Abd El-Aziz, Moaheda E H Eissa, Sara F Ghanem","doi":"10.1007/s10695-025-01459-1","DOIUrl":"10.1007/s10695-025-01459-1","url":null,"abstract":"<p><p>This study evaluated the effects of dietary organic acid (OA) blend on hemato-immunological responses, reproduction capacity, gene expression, and histological features of red tilapia (Oreochromis niloticus × O. mossambicus) broodstock. Four diets were formulated, containing 0 (control), 2, 3, and 4 g OAs kg^-1. The diets were fed to triplicate groups (n = 3) of red tilapia broodstock (75 ± 5.56 g( (mean ± standard deviation (SD)) stocked in 10-m³ concrete tanks at a male to female ratio of 1:3, to satiation, twice a day, for 8 weeks. At the end of the feeding trial, fish in each tank were collected, counted, and weighed. Blood samples were collected from five fish from each tank and used for the determination of hematological and biochemical parameters. The fish were then dissected to study the reproductive performance and reproduction-related genes. The red blood cells (RBCs), hemoglobin (Hb), packed cell volume (PCV%), mean corpuscular volume (MCV), and lysozyme activity were significantly increased (P < 0.05) with increasing dietary OAs to 4 g kg^-1. Mean corpuscular hemoglobin (MCH), white blood cells (WBCs), total protein, albumin, and globulin), and digestive enzyme activity values leveled off or slightly decreased (P > 0.05) at OA levels above 3 g kg^-1. Optimum liver enzyme activity was obtained at 2 g kg^-1 OA. The reproductive hormones: testosterone (T), follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), and progesterone (Prog), organo-somatic index (GSI), reproductive performance, and the expression of reproductive genes (vasa, nanos1a, nanos2, dnd1, pum1, amh, and VTG) exhibited dose-dependent responses (P < 0.05), suggesting that 4 g OA kg^-1 boosted the optimum reproductive performance. In conclusion, about 2-3 g OA kg^-1 diet can improve the hemato-biochemical parameters, immune response, antioxidant status, and digestive enzyme activity in red tilapia broodstock, whereas 4 g kg^-1 could accelerate their reproductive performance through upregulation of reproductive genes.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"41"},"PeriodicalIF":2.5,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11794372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of the microalgae Tisochrysis lutea in a \"green water\" system increases the absorption area in the intestine of larvae of the seahorse Hippocampus reidi Ginsburg, 1933.","authors":"Elen Monique de Oliveira Sousa, Nayara Yoshimini de Oliveira, Ianka Agra da Silva, Renata Ávila Ozório, Emilly Monteiro Lopes, Maurício Laterça Martins, Mônica Yumi Tsuzuki","doi":"10.1007/s10695-024-01428-0","DOIUrl":"10.1007/s10695-024-01428-0","url":null,"abstract":"<p><p>The study aimed to investigate the influence of different microalgae used in green water culture on the intestinal morphology of the seahorse Hippocampus reidi during the first 15 days of life. Four treatments were tested in triplicate, consisting of two microalgae (Tisochrysis lutea-ISO and Chaetoceros muelleri-CHO), used either individually (TISO and TCHO) or combined (TIC 1:1) in larval rearing water, and a treatment without microalgae (TWM). Larvae (6.68 ± 0.55 mm) were fed copepods (Parvocalanus crassirrostris; 2 ind mL^-1) and rotifers (Brachionus rotundiformis; 8 ind mL^-1) from the first to the seventh day, and from the eighth day onwards, with Artemia sp. nauplii (2 ind mL^-1). For the analysis of intestinal histomorphometry, histological analysis of the tissue was performed on day 0 (initial) and after 7 and 15 days. The results demonstrate a significant increase in weight and weight gain of the larvae on the 15th day in the treatments with the addition of the microalgae T. lutea, whether alone or combination with other microalgae, compared to TWM. In relation the intestinal histomorphometry of larvae, the ANCOVA revealed that, on the 7th and 15th day, only the treatments with microalgae significantly affected the surface area of the intestinal villi. The intestinal histomorphometry of larvae from TISO showed higher values of villus height (85.29 ± 1.23 µm²) compared to TWM (69.42 ± 2.82 µm²) and TCHO (76.92 ± 2.23 µm²) (P < 0.05) but did not show significant differences compared to TIC (80.127 ± 2.08 µm²), which reflected on the villi surface area. Therefore, larvae from TISO (3603.43 ± 151.31 µm²) and TIC (3550.29 ± 120.99 µm²) showed significantly higher values of this parameter compared to TWM (3026.79 ± 147.64 µm²) and TCHO (3069.09 ± 126.18 µm²) (P < 0.05). In conclusion, the protocol for H. reidi larvae rearing in a green water system with the addition of the microalgae T. lutea resulted in an increase in the intestinal absorption area, resulting in improved performance of the larvae.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"20"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and sex affect respiratory responses to temperature and dissolved oxygen in the air-breathing fishes Betta splendens and Trichopodus trichopterus.","authors":"My Phuong Le, Warren Burggren, Gil Martinez-Bautista","doi":"10.1007/s10695-024-01411-9","DOIUrl":"10.1007/s10695-024-01411-9","url":null,"abstract":"<p><p>Ventilation frequencies of the gills (f_G) and the air-breathing organ (f_ABO) were measured in juveniles and adults of the air-breathing betta (Betta splendens) and the blue gourami (Trichopodus trichopterus) in response to temperature and hypoxia. Ventilatory rates were evaluated after 1 h of exposure to 27 °C (control), 23 and 31 °C (PO₂ = 21.0 kPa), after acute temperature changes (ATC) from 23 to 27, and 27 to 31 °C, and under progressive hypoxia (PH; PO₂ =  ~ 21 to 2.5 kPa). Complex, multi-phased ventilatory alterations were evident across species and experimental groups revealing different stress responses and shock reactions (e.g., changes in temperature sensitivity (Q₁₀) of f_G between 1-h exposure and ACT in both species). Female and male gourami showed differences in Q₁₀ over the temperature range 23-31 °C. No such Q₁₀ differences occurred in betta. Juveniles of both species showed higher Q₁₀ for f_ABO (~ 3.7) than f_G (~ 2.2). Adult fish exhibited variable Q₁₀s for f_G (~ 1.5 to ~ 4.3) and f_ABO (~ 0.8 to ~ 15.5) as a function of temperature, suggesting a switch from aquatic towards aerial ventilation in response to thermal stress. During PH, juveniles from both species showed higher f_G than adults at all oxygen levels. Females from both species showed higher f_G compared with males. Collectively, our results suggest that environmental cues modulate ventilatory responses in both species throughout ontogeny, but the actual responses reflect species-specific differences in natural habitat and ecology. Finally, we strongly suggest assessing physiological differences between male and female fish to avoid masking relevant findings and to facilitate results interpretation.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"27"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of global warming effects on juvenile rainbow trout: focus on immunohistochemistry and osmoregulation.","authors":"Zehra Alkan, Boran Karataş, Ahmet Sepil","doi":"10.1007/s10695-024-01431-5","DOIUrl":"10.1007/s10695-024-01431-5","url":null,"abstract":"<p><p>The negative effects of global warming also directly affect aquatic populations. Consequences such as evaporation due to chronic temperature increase, increase in salinity, and increase in stock density per unit volume are potential stress factors. While creating the trial design, an attempt was made to simulate the effects of global warming, especially on species living in salty and brackish water biotopes. In this study, changes in the gills of rainbow trout (Oncorhynchus mykiss) acclimated to 0, 20, and 38 ‰ of saline in the laboratory were examined histologically and immunohistochemically and blood serum osmolarity. In addition, the water temperature was changed, and experiments were carried out at 16, 19, and 22 °C for each salinity group in parallel with the increase in salinity. However, to simulate the decrease in water volume and intensive stocking due to the potential impact of climate change, the study was carried out using 15 fishes in low-volume aquariums (45 L). Tap water that had been kept for at least 3 days was used in the aquariums. To protect the water quality, independent aquariums with sponge filters were used, and since the aim was to keep dissolved oxygen low, no ventilation system other than the sponge filter was used. In order to minimize the deterioration in water quality during the trial, a 15% water change was performed by performing a bottom flush every 4 days and water of the same temperature and salinity was added as much as the reduced volume. In addition, since increasing stock density due to temperature increase and water decrease will cause the amount of dissolved oxygen to decrease, pure oxygen was not entered into any tank throughout the experiment, and the concentration was requested to be at a low level (7 ± 0.13 mg/L) in all groups. The trials were terminated at the end of the 71st day. Increased serum osmolarity values were observed due to the increase in salinity, and the highest serum osmolarity value was measured at 644 mOsm/kg in the 38 ‰ salinity group. Differences between the groups were found to be statistically significant (p < 0.05). It was observed that the number of cells containing Na⁺/K⁺-ATPase increased depending on salinity. Also, the number of chloride cells reached the maximum level in the 38 ‰ salinity group. Due to increasing salt levels, an increase in mucus cells, limited onset hyperplasia, aneurysm, lamellar separation, and necrosis were observed in the gill tissue.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"15"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aquaculture potential of Atlantic wolffish (Anarhichas lupus): stress and physiological responses to acute handling.","authors":"Ida Hedén, Jonathan Armand Charles Roques, Marica Andersson, Niklas Warwas, Raneesha de Fonseka, Darragh Doyle, James Hinchcliffe, Elisabeth Jönsson, Kristina Sundell, Henrik Sundh","doi":"10.1007/s10695-025-01456-4","DOIUrl":"10.1007/s10695-025-01456-4","url":null,"abstract":"<p><p>The Atlantic wolffish (Anarhichas lupus) is a cold-water species with the potential to diversify aquaculture in Northern countries. Few studies have investigated the stress physiology of Atlantic wolffish, and the current knowledge on stress in wolffish species is largely derived from the closely related spotted wolffish (Anarhichas minor). In the current study, Atlantic wolffish were exposed to handling stress mimicking common husbandry conditions in aquaculture such as repeated air exposure and net-chasing. Samples were taken prior to stress exposure (pre-stress; control) as well as 5- and 24-h post-stress. A series of primary and secondary acute stress response parameters were assessed: plasma cortisol, glucose and lactate levels, hematological indices (hemoglobin, Hb; hematocrit, Hct; mean corpuscular hemoglobin concentration, MCHC), and osmoregulatory capacity through plasma osmolality and gill Na⁺/K⁺ATPase (NKA) activity. Other secondary stress responses with implications for fish health and welfare are intestinal integrity and transport functions. These parameters were assessed using the Ussing chamber technique. The cortisol peak values were low in comparison to other fish species studied after acute handling stress and occurred as late as 24 h post-stress, suggesting that Atlantic wolffish is a slow and low cortisol responder. Plasma glucose remained stable, whereas lactate concentrations significantly decreased between 5 and 24 h after stress. There was no effect on pH, Hb, or Hct, although a significant increase in MCHC was found after 5 h and 24 h, originating from a small increase in Hb. This result suggests a minor increase in Hb synthesis after stress exposure. The intestinal integrity and transport functions as well as gill NKA-activity remained unchanged after stress exposure. In conclusion, Atlantic wolffish appears to exhibit a relatively moderate stress response, characterized by a slow and low primary stress response and minimal secondary effects following husbandry-related acute stress. These findings contribute to the understanding of the species' potential for development as a candidate for marine, cold-water aquaculture.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"39"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane progesterone receptor e (paqr9) is necessary for chorion elevation in zebrafish.","authors":"Md Razain Tanvir, Takumi Mouri, Eisei Tsutsumi, Umme Habiba Mustary, Md Almamun Farid, Md Forhad Hossain, Yuki Omori, Chihiro Yamamoto, Akiteru Maeno, Toshinobu Tokumoto","doi":"10.1007/s10695-024-01435-1","DOIUrl":"10.1007/s10695-024-01435-1","url":null,"abstract":"<p><p>Paqr9 is a gene encoding membrane progestin receptor e (mPRe), the fifth subtype of the five mPR subtypes, and is currently classified a member of the progestin and adipoQ receptor (PAQR) family, which consists of 11 genes. To elucidate the physiological functions of the mPR subtypes, we established gene knockout (KO) fish via genome editing of seven paqr genes in zebrafish and analyzed their phenotypes. The null-mutant strain of paqr9 (paqr9^-/-) that we established in this study presented reduced chorion elevation and a high percentage of abnormal embryos. Embryos exhibit various kinds of abnormal morphology, which are thought to be caused by insufficient elevation of the chorion. Immunohistochemical staining of ovaries with an anti-Paqr9 antibody revealed that Paqr9 was expressed in the periplasm of oocytes and the surface of chorion in the wild type, whereas signals were absent in paqr9^-/- zebrafish. In histological sections, the periplasmic connection between the oocyte plasma membrane and chorion was absent in paqr9^-/- oocytes. The number of cortical alveoli (CA) that are responsible for chorion elevation was significantly reduced in paqr9^-/- zebrafish. SEM revealed that fiber-supported knob-like structures (KSs) on the chorion were absent in paqr9^-/- zebrafish. These results indicate that Paqr9 is required for the preparation of CA during oogenesis. Insufficient formation of the chorion resulted in the abnormal development of embryos.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"1"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stranded heavy fuel oil exposure causes deformities, cardiac dysfunction, and oxidative stress in marine medaka Oryzias melastigma using an oiled-gravel-column system.","authors":"Zhonglei Ju, Xishan Li, Xin Li, Cen Liang, Zhu Xu, Huishu Chen, Deqi Xiong","doi":"10.1007/s10695-024-01437-z","DOIUrl":"10.1007/s10695-024-01437-z","url":null,"abstract":"<p><p>Heavy fuel oil (HFO) stranded on the coastline poses a potential threat to the health of marine fish after an oil spill. In this study, an oiled-gravel-column (OGC) system was established to investigate the toxic effects of stranded HFO on marine medaka Oryzias melastigma. HFO 380# (sulfur content 2.9%) was chosen as one type of high sulfur fuel oil for acute toxicity tests. The marine medaka larvae were exposed to the OGC system effluents with oil loading rates of 0 (control), 400, 800, 1600, and 3200 µg HFO/g gravel for 144 h, respectively. Results showed that a prevalence of blue sac disease signs presented teratogenic effects, including decreased circulation, ventricular stretch, cardiac hemorrhage, and pericardial edema. Moreover, the treatments (800, 1600, and 3200 µg oil/g gravel) induced severe cardiotoxicity, characterized by significant bradycardia and reduced stroke volume with an overt decrease in cardiac output. Additionally, the antioxidant enzyme activities, including catalase (CAT), peroxidase (POD), and glutathione S-transferase (GST) were significantly upregulated at 800-3200 µg oil/g gravel except for a marked inhibition of CAT activity at 3200 µg oil/g gravel. Furthermore, significantly elevated protein carbonyl (PCO) levels were detected, suggesting that the organisms suffered severe protein oxidative damage subjected to the exposure. Overall, stranded HFO 380# exposure activated the antioxidant defense system (up-regulated POD and GST activities) of marine medaka and disrupted CAT activity, which could result in an oxidative stress state (elevated PCO levels) and might further contribute to cardiac dysfunction, deformities, and mortality.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"29"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}