Fish Physiology and Biochemistry最新文献

{"title":"Effects of homochromatic lights on the physiological responses and morphochromatic traits of scarlet badis, Dario dario (Hamilton, 1822).","authors":"N Siva, Gouranga Biswas, Sweta Pradhan, Arunashri A, Paramita B Sawant","doi":"10.1007/s10695-025-01523-w","DOIUrl":"https://doi.org/10.1007/s10695-025-01523-w","url":null,"abstract":"<p><p>Aquarium fish keeping ranks as the world's second most popular hobby, with a global trade value of 18-20 billion USD annually. Despite its popularity, aquarium lighting is often used for aesthetics without considering its biological effects on fish. This study examined how homochromatic lights affect growth, feed utilization, survival, stress markers, hormone levels, and skin pigmentation in the ornamental fish Dario dario (scarlet badis). Ten male fish (92.79 ± 4.33 mg) were stocked per tank and exposed to six light treatments: red light (RL), blue light (BL), green light (GL), white light (WL), ambient light (AL), and dark (D) for 30 days under a 12:12-h (light:dark) photoperiod. Among treatments, GL significantly enhanced growth, feed efficiency, and survival. Stress biomarkers, namely superoxide dismutase activity, catalase activity, glucose, and cholesterol contents were significantly lower (P < 0.01) under GL, WL, and AL. Fish under GL also had elevated 11-keto testosterone and reduced cortisol levels (P < 0.01), indicating hormonal balance. Visibly, GL-exposed fish displayed more vibrant red bands, confirmed through skin chromaticity analysis and microscopic observation of pigment dispersion in chromatophores. In summary, green light created a favourable environment for D. dario, improving growth, stress reduction, and ornamental value.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"118"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin receptor-mediated signaling pathways drive time-dependent physiological recovery from freezing in Perccottus glenii.","authors":"Zhaoyang Ning, Cunrun Ye, Yingqiao Chen, Jiajun Zhou, Xiaoyu Zhao, Ye Huang, Zhitao Liu, Weijie Mu","doi":"10.1007/s10695-025-01516-9","DOIUrl":"https://doi.org/10.1007/s10695-025-01516-9","url":null,"abstract":"<p><p>Freeze tolerance in fish has been associated with various physiological adaptations; however, the role of the melatonin (MT) system in fish freeze recovery remains poorly understood. This study investigates the role of MT and its receptors in the recovery phase from hibernation in the fish species Perccottus glenii. The results showed that during the recovery phase, MT content in untreated P. glenii increased gradually, with significantly higher levels at 12 h than at 4 h. MT injection facilitated the recovery of P. glenii, as evidenced by significant differences in hibernation recovery behaviors between injected and non-injected fish. In the liver during recovery, the mRNA levels of AANAT and HIOMT, key enzymes for MT secretion, were higher at 12 h. The expressions of MT receptors MTNR1A, MTNR1B, and MTNR1C were also upregulated at 12 h. In the liver, the expressions of PKC and ERK increased significantly at 12 h, suggesting MT role through the receptor pathway. Experiments with primary hepatocytes indicated that the MT receptor promoted PKC expression. In the liver during recovery, ERK and PKC protein levels were higher at 12 h than at 4 h, while PKA was lower. Various expression changes were observed for PKG, NF-κB, AKT, CAMK1, and CREB in different treatment groups. There were significant differences in the mRNA expressions of PKG, AKT, and CAMK1 between 12 and 4 h recovery cells and in CREB levels among the control, 4 h, and 12 h groups. The findings suggest that MT is involved in the hibernation recovery process, and its receptor-mediated signaling pathways play important roles, with mechanisms that may be time-dependent. The present study highlights the importance of the MT system in fish freeze recovery and offers a potential target for further research on improving fish survival in cold conditions.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"116"},"PeriodicalIF":2.5,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of urea nitrogen fertilizer on the physiology, behavior, and histology of juvenile rainbow trout.","authors":"Tomas Makaras, Julija Razumienė, Vidutė Gurevičienė, Gintarė Sauliutė, Nataliia Matviienko, Mykhailo Kozij, Milda Stankevičiūtė","doi":"10.1007/s10695-025-01528-5","DOIUrl":"https://doi.org/10.1007/s10695-025-01528-5","url":null,"abstract":"<p><p>Excessive use of nitrogen (N) fertilizers can induce metabolic alterations in fish by disrupting nitrogen metabolism and depleting energy reserves needed to maintain nitrogen homeostasis. This study evaluated the acute (96-h LC50) and prolonged (14-day) effects of urea nitrogen fertilizer (5 g L⁻¹ was determined to be the lowest tested concentration with no observable effects) on the physiological, histological, and behavioral responses of rainbow trout (Oncorhynchus mykiss) juveniles. Significant increases in internal urea levels were observed in the blood, gills, and kidneys of exposed fish, alongside decreased glucose levels in blood and renal tissues. The kidneys showed time-dependent changes in urea levels, while circulating glucose levels varied across tissues, with the kidneys exhibiting the most pronounced response. These variations reflect physiological compensatory mechanisms in response to urea-N exposure. A 14-day exposure to urea caused both functional and structural changes in the gills, liver, and kidneys. Increased swimming activity was also noted, persisting throughout the exposure period. Water urea measurements throughout 14-days period were significant but less informative for identifying patterns associated with urea level changes. Further research is needed to investigate the effects of other nitrogen compounds, particularly those associated with eutrophication, and to optimize the use of these biomarkers for indicating metabolic consequences across various biological matrices.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"115"},"PeriodicalIF":2.5,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of galanin in reproduction of male tilapia, Oreochromis mossambicus.","authors":"Ashwini Biradar, C B Ganesh","doi":"10.1007/s10695-025-01527-6","DOIUrl":"https://doi.org/10.1007/s10695-025-01527-6","url":null,"abstract":"<p><p>Galanin(GAL) is recognised for its involvement in various physiological, behavioural, and neuroendocrine functions in vertebrates. Yet, the impact of this peptide on spermatogenesis in fish remains understudied. In this investigation, the administration of 5 µg of GAL receptor agonist (GAL-A) to the tilapia Oreochromis mossambicus did not significantly alter the numbers of different germ cells except for a significant rise in the number of late spermatids compared to the control group. By contrast, treatment with 20 µg of GAL-A caused a significant rise in all germ cell counts and tubule and lumen areas and a significant reduction in the area of interstitium relative to the control group. The numbers of spermatogonia-A, primary and secondary spermatocytes, and early spermatids showed a strong positive association with regard to their respective cyst size. Furthermore, fish administered 20 µg of GAL-A exhibited significant increases in the percentage of GnRH-immunoreactive fibres within the nucleus lateralis tuberis and proximal pars distalis of the pituitary gland, concomitant with significantly higher serum levels of LH, and the intensity of androgen receptor protein expression in the testis when compared to control groups. The in vitro experimental results revealed a significantly increased level of 11-KT in the testis treated with 30 or 300 µM of GAL-A when compared to the control group. Together, these results demonstrate for the first time that GAL-A enhances spermatogenesis and steroidogenesis in the testis. GAL-A may influence these effects through the hypothalamic-pituitary axis and directly at the testicular level in teleosts.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"117"},"PeriodicalIF":2.5,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorative potential of dietary supplements, ZnO-K, citrus essential oil, and pumpkin seed oil, on sperm quality in Nile tilapia: Insights from CASA, DNA integrity, antioxidant enzymes, and gene expressions.","authors":"Marwa M Ali, Kamal Fathy Elboray, Engy T Megahed, Hany T Abu-Taleb, Alshimaa E Elsayed, Eman Y Mohammady, Mona S Amer, Soliman A Morsi, Eman M Abbas, Mohamed S Hassaan, Hosam Elsaied","doi":"10.1007/s10695-025-01529-4","DOIUrl":"10.1007/s10695-025-01529-4","url":null,"abstract":"<p><p>Improving sperm quality is essential for enhancing the reproductive efficiency of Oreochromis niloticus. This study evaluated the effect of three dietary antioxidant supplements, kaolinite-doped zinc oxide (ZnO-K), citrus essential oil (CEO), and pumpkin seed oil (PSO), on sperm quality parameters. A suite of integrative assessments, including Computer-Assisted Semen Analysis (CASA) parameters, spermatozoa DNA integrity, antioxidant enzyme assays, and gene expression profiling, was applied to validate sperm quality. A total of 192 adult males (mean weight 421.31 g ± 30.41 SD) were assigned to four dietary groups, each with three replicates. The control group received a basal diet without supplementation. The experimental groups were fed diets supplemented with ZnO-K (0.06 g kg⁻¹), CEO (10 g kg⁻¹), or PSO (15 g kg⁻¹). ZnO-K supplementation significantly (P < 0.05) increased milt volume (1.40 ± 0.10 ml) and sperm concentration (5.676 × 10⁹ sperm ml^-1), along with marked improvements in CASA parameters (sperm motility and velocity) and DNA integrity. Antioxidant enzymes, catalase, CAT, glutathione peroxidase, GPX, and superoxide dismutase, SOD, were also significantly elevated in the ZnO-K group, recording activity values of 47.333 ± 1.452 U ml^-1 milt, 65.667 ± 5.547 mU ml^-1 milt and 60.667 ± 3.382 U ml^-1 milt, respectively. Gene expression analysis revealed that CEO induced the highest upregulation of HSP70, while both CEO and ZnO-K significantly upregulated CC chemokine expression compared to control. In contrast, PSO consistently showed the lowest performance across all measured parameters. Collectively, ZnO-K supplementation most effectively enhanced sperm physiological and biochemical quality, whereas CEO predominantly supported cellular stress protection through gene expression modulation. These findings offered practical implications for optimizing artificial reproduction and sperm cryopreservation strategies in tilapia aquaculture.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"114"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12185577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of glyptosternum maculatum (sisoridae: siluriformes) provided insights into sexual dimorphism, Xizang, China.","authors":"Haoming He, Mengyu Wu, Zhuangzhuang Wang, Jianshe Zhou, Wanliang Wang","doi":"10.1007/s10695-025-01520-z","DOIUrl":"https://doi.org/10.1007/s10695-025-01520-z","url":null,"abstract":"<p><p>Glyptosternum maculatum, designated as a second-class nationally protected aquatic species in Xizang, China, has been categorized as critically endangered on the Red List of China's Vertebrates. This species exhibits significant sexual dimorphism. The primary objective of this research was to explore and analyze the genetic components underlying sexual variations in G. maculatum. In this investigation, transcriptomic methodologies were utilized to identify potential genes contributing to sexual dimorphism in G. maculatum. The transcriptomic analysis yielded 9788 differentially expressed genes (DEGs) between males and females, with 5490 upregulated and 4157 downregulated. Enrichment analysis via the KEGG pathway identified substantial enrichment of DEGs in six pathways central to sex determination, sexual differentiation, and gonadal development. These pathways were grouped into three primary signaling pathways: P450 pigment synthesis, steroid biosynthesis, and energy metabolism. Functional annotation additionally pinpointed 16 genes exhibiting high expression levels during sexual differentiation, including cyp19a, dmrtX1, SOX9, kiss2, and FSHR. These genes, previously substantiated in other teleost species, were confirmed to play crucial roles in sex determination. This study offers substantial theoretical foundation and empirical data, thereby facilitating future endeavors in identifying sex-determining genes, dissecting their functional aspects, and advancing the development of all-male offspring in G. maculatum.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"113"},"PeriodicalIF":2.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryonic thermal environments drive plasticity in gene expression.","authors":"Anthony A Snead, Corey R Quackenbush, Shawn Trojahn, Anna L McDonald, Luana S F Lins, Chris Cornelius, Paula E Adams, Dengke Ma, Yuying Hsu, Eric Haag, Frédéric Silvestre, Akira Kanamori, Ryan L Earley, Joanna L Kelley","doi":"10.1007/s10695-025-01522-x","DOIUrl":"10.1007/s10695-025-01522-x","url":null,"abstract":"<p><p>When embryos experience different environments than their parents, plasticity can enable the development of alternate phenotypes that confer higher fitness in the new conditions. Temperature-induced plasticity could be especially critical for species that inhabit areas with considerable thermal variation. We studied transcriptional variation in embryos of mangrove rivulus (Kryptolebias marmoratus)-a self-fertilizing hermaphroditic, eurythermal fish that resides in notoriously spatiotemporally variable mangrove forests-exposed to different thermal regimes during development. To study transcriptional plasticity, we first improved the genome assembly to chromosome length scaffolds (N50 of 28.17 Megabases). Whole transcriptome sequencing revealed that both temperature and developmental timing modulated embryonic gene expression. We found few differences in gene expression between embryos incubated in cold and warm conditions and assessed before the temperature-sensitive period of development, indicating high resistance to stochastic changes in gene expression early in development. Replicate embryos exposed to cold temperatures and sampled after the temperature-sensitive period showed less variation in gene expression than those sampled before, suggesting canalization of the plastic response. DNA replication/repair, organelle, and gas transport pathways were upregulated while nervous system development, cell signaling, and cell adhesion were downregulated in cold-exposed compared to warm-exposed embryos sampled after the temperature-sensitive period. These plastic shifts in gene expression could have major implications for reorganizing the phenotype (e.g., apoptosis, mitosis) in response to environmental changes occurring within a generation.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"111"},"PeriodicalIF":2.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cottonseed protein concentrate as fish meal substitution in fish diet: a review.","authors":"You Xing Xu, Zhi Li Ding, Xing Han He, Hui Fei","doi":"10.1007/s10695-025-01525-8","DOIUrl":"10.1007/s10695-025-01525-8","url":null,"abstract":"<p><p>Aquaculture has been challenged to explore fish meal (FM) alternative proteins to develop efficient feed formulations that foster a sustainable future growth. Among them, cottonseed protein concentrate (CPC) is an innovative source of substantial non-edible protein with a high output, derived from agricultural by-products. It possesses enhanced nutritional benefits and appealing taste, attributed to the progressive advancements in cottonseed processing machinery and production techniques. Moreover, the levels of anti-nutritional factors (ANFs) such as gossypol are minimized, rendering CPC more easily digestible and assimilable by fish. The effect of dietary CPC on the growth performance, flesh quality, hematological and antioxidant indices, intestinal health and microbiota, and immune response of farmed fish has been well demonstrated in various fish species, suggesting the appropriate level for replacing FM with CPC varied across the fish species and the FM contents in the basic diet. Nevertheless, certain issues including the imbalanced amino acid composition, and the cumulative presence of gossypol when the diet containing abundant CPC should be concerned. In this review, we provide an overview of the nutritional components, key ANFs of CPC, and explore its use in fish nutrition. Furthermore, we deliberate on effective strategies to enhance its efficacy as a protein supplement in fish feed. This review establishes a scholarly groundwork and offers pragmatic direction for the investigation and implementation of CPC in fish feeding practices, thereby advocating for a decrease in the utilization of FM in aquaculture.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"112"},"PeriodicalIF":2.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progestogen is a potential trigger initiating sex inversion in rice field eel (Monopterus albus).","authors":"Xiancheng Qu, Jiaoyun Jiang, Min Li, Jianhua Ye, Lisa Pan, Qinhua Zhang, Guofang Zhong, Xinchao Zhu, Yifang Du, Xianhui Li","doi":"10.1007/s10695-025-01524-9","DOIUrl":"10.1007/s10695-025-01524-9","url":null,"abstract":"<p><p>The rice field eel or swamp eel (Monopterus albus) is a hermaphroditic freshwater teleost fish that transitions from a functional female to a functional male. However, the biological mechanism of sex inversion in this species remains unclear. Here we clone the 5' proximal flanking region of the rice field eel dmrt1 gene and find that the progesterone response element (PRE) and androgen response element (ARE) uniquely exist in this region. More importantly, based on reporter gene analysis, in vitro tissue culture, and progestogen hormone level assays, we reveal that progestogen is the prerequisite for the sex inversion of the rice field eel, and androgen plays an important role in the positive regulation of the sex inversion process. Collectively, our results suggest that progestogen is the trigger that initiates the rice field eel's sex trans-differentiation during final oocyte maturation and clarifies the biological mechanism of sex inversion in the rice field eel.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"109"},"PeriodicalIF":2.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rainbow trout welfare: comparing stunning methods in winter and summer.","authors":"Roberto González-Garoz, Almudena Cabezas, Montserrat Fernández-Muela, Andrea Martínez Villalba, Elisabeth González de Chávarri, Morris Villarroel, Álvaro De la Llave-Propín, Jesús De la Fuente, Rubén Bermejo-Poza, María Teresa Díaz","doi":"10.1007/s10695-025-01526-7","DOIUrl":"10.1007/s10695-025-01526-7","url":null,"abstract":"<p><p>This study investigates the effects of three stunning methods: thermal shock (TS), electric shock at 200 mA 2 s (ES2), and electric shock at 400 mA 0.5 s followed by 200 mA 1.5 s (ES4) - both electric shock treatments followed by thermal shock - on the stress response and energy metabolism of rainbow trout (Oncorhynchus mykiss) during winter and summer. A significant interaction between stunning method and season was observed for blood cortisol levels. In summer, cortisol concentrations were elevated regardless of the stunning method, whereas in winter, the ES4 method resulted in the lowest cortisol levels. Seasonal variation significantly influenced stress response in trout, with summer conditions leading to higher cortisol levels, darker skin pigmentation, and reduced liver glycogen and lipid reserves. Increased metabolic activity during summer was associated with enhanced reactive oxygen species (ROS) production, which triggered the upregulation of key antioxidant enzymes (sod, gpx, cat, gst). The results suggest that the choice of stunning method is critical in mitigating stress, with electrical stunning combined with thermal shock being more effective than thermal shock alone, particularly under elevated temperatures. These findings underscore the importance of optimizing stunning practices to improve fish welfare, especially in the context of climate change. Understanding the interplay between seasonal and procedural stressors provides valuable insights for improving aquaculture management and sustainability.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"110"},"PeriodicalIF":2.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}