Fish Physiology and Biochemistry最新文献

Transcriptome analysis of glyptosternum maculatum (sisoridae: siluriformes) provided insights into sexual dimorphism, Xizang, China. 西藏斑纹胸骨（剑虫科：剑虫形目）的转录组分析提供了性别二态性的见解。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-06-17 DOI: 10.1007/s10695-025-01520-z

Haoming He, Mengyu Wu, Zhuangzhuang Wang, Jianshe Zhou, Wanliang Wang

{"title":"Transcriptome analysis of glyptosternum maculatum (sisoridae: siluriformes) provided insights into sexual dimorphism, Xizang, China.","authors":"Haoming He, Mengyu Wu, Zhuangzhuang Wang, Jianshe Zhou, Wanliang Wang","doi":"10.1007/s10695-025-01520-z","DOIUrl":"https://doi.org/10.1007/s10695-025-01520-z","url":null,"abstract":"Glyptosternum maculatum, designated as a second-class nationally protected aquatic species in Xizang, China, has been categorized as critically endangered on the Red List of China's Vertebrates. This species exhibits significant sexual dimorphism. The primary objective of this research was to explore and analyze the genetic components underlying sexual variations in G. maculatum. In this investigation, transcriptomic methodologies were utilized to identify potential genes contributing to sexual dimorphism in G. maculatum. The transcriptomic analysis yielded 9788 differentially expressed genes (DEGs) between males and females, with 5490 upregulated and 4157 downregulated. Enrichment analysis via the KEGG pathway identified substantial enrichment of DEGs in six pathways central to sex determination, sexual differentiation, and gonadal development. These pathways were grouped into three primary signaling pathways: P450 pigment synthesis, steroid biosynthesis, and energy metabolism. Functional annotation additionally pinpointed 16 genes exhibiting high expression levels during sexual differentiation, including cyp19a, dmrtX1, SOX9, kiss2, and FSHR. These genes, previously substantiated in other teleost species, were confirmed to play crucial roles in sex determination. This study offers substantial theoretical foundation and empirical data, thereby facilitating future endeavors in identifying sex-determining genes, dissecting their functional aspects, and advancing the development of all-male offspring in G. maculatum.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"113"},"PeriodicalIF":2.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Embryonic thermal environments drive plasticity in gene expression. 胚胎热环境驱动基因表达的可塑性。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-06-10 DOI: 10.1007/s10695-025-01522-x

Anthony A Snead, Corey R Quackenbush, Shawn Trojahn, Anna L McDonald, Luana S F Lins, Chris Cornelius, Paula E Adams, Dengke Ma, Yuying Hsu, Eric Haag, Frédéric Silvestre, Akira Kanamori, Ryan L Earley, Joanna L Kelley

{"title":"Embryonic thermal environments drive plasticity in gene expression.","authors":"Anthony A Snead, Corey R Quackenbush, Shawn Trojahn, Anna L McDonald, Luana S F Lins, Chris Cornelius, Paula E Adams, Dengke Ma, Yuying Hsu, Eric Haag, Frédéric Silvestre, Akira Kanamori, Ryan L Earley, Joanna L Kelley","doi":"10.1007/s10695-025-01522-x","DOIUrl":"10.1007/s10695-025-01522-x","url":null,"abstract":"When embryos experience different environments than their parents, plasticity can enable the development of alternate phenotypes that confer higher fitness in the new conditions. Temperature-induced plasticity could be especially critical for species that inhabit areas with considerable thermal variation. We studied transcriptional variation in embryos of mangrove rivulus (Kryptolebias marmoratus)-a self-fertilizing hermaphroditic, eurythermal fish that resides in notoriously spatiotemporally variable mangrove forests-exposed to different thermal regimes during development. To study transcriptional plasticity, we first improved the genome assembly to chromosome length scaffolds (N50 of 28.17 Megabases). Whole transcriptome sequencing revealed that both temperature and developmental timing modulated embryonic gene expression. We found few differences in gene expression between embryos incubated in cold and warm conditions and assessed before the temperature-sensitive period of development, indicating high resistance to stochastic changes in gene expression early in development. Replicate embryos exposed to cold temperatures and sampled after the temperature-sensitive period showed less variation in gene expression than those sampled before, suggesting canalization of the plastic response. DNA replication/repair, organelle, and gas transport pathways were upregulated while nervous system development, cell signaling, and cell adhesion were downregulated in cold-exposed compared to warm-exposed embryos sampled after the temperature-sensitive period. These plastic shifts in gene expression could have major implications for reorganizing the phenotype (e.g., apoptosis, mitosis) in response to environmental changes occurring within a generation.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"111"},"PeriodicalIF":2.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cottonseed protein concentrate as fish meal substitution in fish diet: a review. 棉籽浓缩蛋白在鱼类饲料中替代鱼粉的研究进展。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-06-10 DOI: 10.1007/s10695-025-01525-8

You Xing Xu, Zhi Li Ding, Xing Han He, Hui Fei

{"title":"Cottonseed protein concentrate as fish meal substitution in fish diet: a review.","authors":"You Xing Xu, Zhi Li Ding, Xing Han He, Hui Fei","doi":"10.1007/s10695-025-01525-8","DOIUrl":"https://doi.org/10.1007/s10695-025-01525-8","url":null,"abstract":"Aquaculture has been challenged to explore fish meal (FM) alternative proteins to develop efficient feed formulations that foster a sustainable future growth. Among them, cottonseed protein concentrate (CPC) is an innovative source of substantial non-edible protein with a high output, derived from agricultural by-products. It possesses enhanced nutritional benefits and appealing taste, attributed to the progressive advancements in cottonseed processing machinery and production techniques. Moreover, the levels of anti-nutritional factors (ANFs) such as gossypol are minimized, rendering CPC more easily digestible and assimilable by fish. The effect of dietary CPC on the growth performance, flesh quality, hematological and antioxidant indices, intestinal health and microbiota, and immune response of farmed fish has been well demonstrated in various fish species, suggesting the appropriate level for replacing FM with CPC varied across the fish species and the FM contents in the basic diet. Nevertheless, certain issues including the imbalanced amino acid composition, and the cumulative presence of gossypol when the diet containing abundant CPC should be concerned. In this review, we provide an overview of the nutritional components, key ANFs of CPC, and explore its use in fish nutrition. Furthermore, we deliberate on effective strategies to enhance its efficacy as a protein supplement in fish feed. This review establishes a scholarly groundwork and offers pragmatic direction for the investigation and implementation of CPC in fish feeding practices, thereby advocating for a decrease in the utilization of FM in aquaculture.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"112"},"PeriodicalIF":2.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progestogen is a potential trigger initiating sex inversion in rice field eel (Monopterus albus). 黄鳝（Monopterus albus）的孕激素是引发性反转的潜在因素。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-06-09 DOI: 10.1007/s10695-025-01524-9

Xiancheng Qu, Jiaoyun Jiang, Min Li, Jianhua Ye, Lisa Pan, Qinhua Zhang, Guofang Zhong, Xinchao Zhu, Yifang Du, Xianhui Li

引用次数: 0

Rainbow trout welfare: comparing stunning methods in winter and summer. 虹鳟鱼福利：比较冬季和夏季的惊艳方法。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-06-09 DOI: 10.1007/s10695-025-01526-7

Roberto González-Garoz, Almudena Cabezas, Montserrat Fernández-Muela, Andrea Martínez Villalba, Elisabeth González de Chávarri, Morris Villarroel, Álvaro De la Llave-Propín, Jesús De la Fuente, Rubén Bermejo-Poza, María Teresa Díaz

{"title":"Rainbow trout welfare: comparing stunning methods in winter and summer.","authors":"Roberto González-Garoz, Almudena Cabezas, Montserrat Fernández-Muela, Andrea Martínez Villalba, Elisabeth González de Chávarri, Morris Villarroel, Álvaro De la Llave-Propín, Jesús De la Fuente, Rubén Bermejo-Poza, María Teresa Díaz","doi":"10.1007/s10695-025-01526-7","DOIUrl":"10.1007/s10695-025-01526-7","url":null,"abstract":"This study investigates the effects of three stunning methods: thermal shock (TS), electric shock at 200 mA 2 s (ES2), and electric shock at 400 mA 0.5 s followed by 200 mA 1.5 s (ES4) - both electric shock treatments followed by thermal shock - on the stress response and energy metabolism of rainbow trout (Oncorhynchus mykiss) during winter and summer. A significant interaction between stunning method and season was observed for blood cortisol levels. In summer, cortisol concentrations were elevated regardless of the stunning method, whereas in winter, the ES4 method resulted in the lowest cortisol levels. Seasonal variation significantly influenced stress response in trout, with summer conditions leading to higher cortisol levels, darker skin pigmentation, and reduced liver glycogen and lipid reserves. Increased metabolic activity during summer was associated with enhanced reactive oxygen species (ROS) production, which triggered the upregulation of key antioxidant enzymes (sod, gpx, cat, gst). The results suggest that the choice of stunning method is critical in mitigating stress, with electrical stunning combined with thermal shock being more effective than thermal shock alone, particularly under elevated temperatures. These findings underscore the importance of optimizing stunning practices to improve fish welfare, especially in the context of climate change. Understanding the interplay between seasonal and procedural stressors provides valuable insights for improving aquaculture management and sustainability.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"110"},"PeriodicalIF":2.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the impact of salinity on the osmoregulatory organs of Clarias batrachus: a histochemical and gill ultrastructural study. 盐度对batrachus渗透调节器官的影响：组织化学和鳃的超微结构研究。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-06-04 DOI: 10.1007/s10695-025-01521-y

Shifali Hafeez, Fauzia A Sherwani

{"title":"Unraveling the impact of salinity on the osmoregulatory organs of Clarias batrachus: a histochemical and gill ultrastructural study.","authors":"Shifali Hafeez, Fauzia A Sherwani","doi":"10.1007/s10695-025-01521-y","DOIUrl":"https://doi.org/10.1007/s10695-025-01521-y","url":null,"abstract":"Under both natural and cultural environments, the increased water salinity may serve as a significant stressor and have a direct impact on fish metabolism. Therefore, this study assesses the impact of salinity stress on a freshwater fish, Clarias batrachus, when exposed to two different salinity levels (30% and 35% seawater) at three time points (24 h, 3 days and 6 days). Histochemical examination of osmoregulatory organs like skin and gills using Alcian Blue (AB, pH 2.5) and Periodic Acid-Schiff (PAS) staining showed that as salinity and exposure time increased, there was a significant decline in mucus cell numbers, percent area occupancy and the size of both acidic and neutral glycoproteins containing mucus cells. PAS staining also revealed significant histological alterations in kidney tissues, indicating the presence of salinity-induced renal stress. Scanning electron microscopy further revealed structural modifications in chloride cells, underscoring their role in physiological adaptation to varying salinity levels. Even though these adaptations point to a compensatory mechanism, the overall results show that C. batrachus suffers negative effects from prolonged exposure to salinity, including impaired tissue integrity and osmoregulatory efficiency. This study gives significant insights into the physiological challenges encountered by freshwater fish under salinity stress, advancing the knowledge of their adaptive capabilities and limitations.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"108"},"PeriodicalIF":2.5,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amyris balsamifera essential oil promotes anesthesia in Colossoma macropomum (Cuvier, 1818): electrophysiological tools in the indication of a therapeutic window. 香脂精油促进巨像的麻醉（居维叶，1818）：电生理工具在治疗窗口的指示。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-06-03 DOI: 10.1007/s10695-025-01503-0

Axell Timotheo Lima Acioli Lins, Daniella Bastos de Araújo, Luciana Eiró-Quirino, Clarissa Araújo da Paz, Thaysa de Sousa Reis, Luana Vasconcelos de Souza, Alicia Chaves Manito, Mahara de Souza Libório, Gabriela de Paula Goyana, Luiz Fernando Duarte de Andrade Junior, Daniella Rocha Bittencourt, Gabriela Brito Barbosa, Barbarella de Matos Macchi, Moisés Hamoy

{"title":"Amyris balsamifera essential oil promotes anesthesia in Colossoma macropomum (Cuvier, 1818): electrophysiological tools in the indication of a therapeutic window.","authors":"Axell Timotheo Lima Acioli Lins, Daniella Bastos de Araújo, Luciana Eiró-Quirino, Clarissa Araújo da Paz, Thaysa de Sousa Reis, Luana Vasconcelos de Souza, Alicia Chaves Manito, Mahara de Souza Libório, Gabriela de Paula Goyana, Luiz Fernando Duarte de Andrade Junior, Daniella Rocha Bittencourt, Gabriela Brito Barbosa, Barbarella de Matos Macchi, Moisés Hamoy","doi":"10.1007/s10695-025-01503-0","DOIUrl":"https://doi.org/10.1007/s10695-025-01503-0","url":null,"abstract":"Currently, anesthesia is widely used in aquaculture, which raises concerns about the choice of anesthetic agent to be administered. Amyris sandalwood essential oil has muscle relaxant characteristics due to its chemical composition, which indicates its potential as an anesthetic product. Therefore, this study evaluates the anesthetic potential of Amyris balsamifera essential oil in Colossoma macropomum as a therapeutic window through behavioral tests and electrocardiographic recordings. The fish were exposed to Amyris balsamifera essential oil at concentrations of 20 μL L-1, 25 μL L-1, 30 μL L-1, 35 μL L-1,and 40 μL L-1, in immersion baths. The behavioral test showed that can caused a loss of the postural reflex in fish, with a reversible effect and recovery times dependent on the concentration of oil used. In addition, this study showed that sandalwood essential oil has the potential to decrease heart rate and maintain sinus rhythm with a dose-dependent effect. These findings were made possible by behavioral analysis parameters and electrocardiographic recordings. These results demonstrate the existence of a safe therapeutic window for the use of Amyris balsamifera essential oil, since in the present study, there were superficial anesthetic planes with treatments of 20 and 25 µL L-1, characterized by rapid recovery after contact and with little cardiorespiratory depression. And for deep anesthetic planes, treatments with 30 to 40 µL L-1, with greater cardiorespiratory depression and longer latency for recovery.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"107"},"PeriodicalIF":2.5,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of main components of artificial compound feed (ACF) for Hippocampus kuda based on the synergistic expression of biological clock genes. 基于生物钟基因协同表达的海马体人工配合饲料主要成分优化

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-05-31 DOI: 10.1007/s10695-025-01514-x

Yongjian Xu, Bo Yang, Xie Jinyang, Yejing Lou

{"title":"Optimization of main components of artificial compound feed (ACF) for Hippocampus kuda based on the synergistic expression of biological clock genes.","authors":"Yongjian Xu, Bo Yang, Xie Jinyang, Yejing Lou","doi":"10.1007/s10695-025-01514-x","DOIUrl":"10.1007/s10695-025-01514-x","url":null,"abstract":"Feed is very important for fish farming. The appropriate composition and proportion of feed ingredients can promote the growth of fish, maintain normal physiology and behavior, and even improve the resistance ability to disease and stress, etc. The core of artificial compound feed (ACF) is the composition and proportion of lipid, protein, and carbohydrate, which are also the main nutritional components required by fish. Appropriate levels and ratios can promote fish growth and save costs, and the improper would affect the biological clock systems of fish, leading to metabolic abnormalities. This study explored the preparation of ACF for H. kuda. The composition and proportion of the three main nutrients in ACF were screened based on the synchronicity between six pairs of clock genes (Clock, Bmal1, Per1, Per2, Per3, Cry1, and Cry2) in the central and peripheral clock systems, as well as the expression of eight lipid-metabolism genes (Hmgcr, Mvk, Mvd, Lss, Fdps, Cetp, Scap, Srebp1, Srebp2) in the liver and their synergy with liver clock genes. The results showed that, based on several parameters such as gene expression cycle, relative expression level, and top phase appearance time, the best synergy between the central and peripheral circadian clock systems was observed in ACF with crude fat content of 8.80%, crude protein content more than 38.4%, and carbohydrate content of 23.5%. Based on the expression relationship between lipid metabolism genes and circadian clock genes in the liver, it was further clarified that the optimal levels of fat, protein, and carbohydrate were determined with 8.80%, 38.4%, and 23.5%, respectively. After 4 weeks of breeding validation, compared with frozen Mysis, the screened ACF fed for H. kuda showed significant advantages in body length specific growth rate (SGRL), body weight specific growth rate (SGRW), and feed conversion rate (FCR).","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"106"},"PeriodicalIF":2.5,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of progestogen neurosteroids on locomotor activity in zebrafish embryos and larvae. 孕激素神经类固醇对斑马鱼胚胎和幼虫运动活性的影响。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-05-29 DOI: 10.1007/s10695-025-01519-6

Mandarin Mathouchanh, Charles A Lessman

{"title":"Effects of progestogen neurosteroids on locomotor activity in zebrafish embryos and larvae.","authors":"Mandarin Mathouchanh, Charles A Lessman","doi":"10.1007/s10695-025-01519-6","DOIUrl":"10.1007/s10695-025-01519-6","url":null,"abstract":"The steroid hormone progesterone (P4) and related compounds called progestogens are well known for their effects on the reproductive system. However, their physiological roles outside reproduction are less understood. Additionally, there is limited information on the toxicological repercussions of environmental exposure to exogenous progestogens and how such exposure might impact the development and survival of animals living in contaminated habitats. Two progesterone-based neurosteroids, allopregnanolone and tetrahydrodeoxycorticosterone (THDOC), are known to enhance γ-aminobutyric acid type A (GABAA) receptor activity, inducing neuronal hyperpolarization. In this study, locomotor parameters in zebrafish embryos and larvae were used as endpoints to assess the inhibitory effects of pregnane neurosteroids. Specifically, spontaneous tail coiling in embryos at 24 h post-fertilization (hpf) and swimming activity in larvae aged 120-168 hpf were evaluated. Motility assays in embryos showed that P4 increased spontaneous tail coiling, whereas larvae exhibited an anesthetic-like loss of motility. This effect was both age- and dose-dependent for P4, deoxycorticosterone (DOC), 5α-dihydroprogesterone, and the membrane progesterone receptor agonist, ORG-OD-02-0, but not for other steroids tested. Removal of the steroids after the onset of anesthesia led to recovery of motility in larvae, suggesting that the observed effects are not due to a non-specific toxicity. Our results suggest that P4 targets the mPR, which acts in association with the GABAA receptor to mediate the loss of locomotor behavior. This study provides further insight into how neuroactive compounds can affect locomotor behaviors during early developmental stages in nonmammalian species.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"105"},"PeriodicalIF":2.5,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructure and androgen analysis during sex inversion of Brycon orbignyanus reared in captivity. 人工饲养的野Brycon byignyanus性别反转过程中的超微结构和雄激素分析。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-05-28 DOI: 10.1007/s10695-025-01517-8

Patricia Postingel Quirino, Maria Luiza Ribeiro Delgado, Luciane Gomes-Silva, Giovana Souza Branco, Renata Guimarães Moreira, Alexandre Ninhaus-Silveira, Rosicleire Veríssimo-Silveira

{"title":"Ultrastructure and androgen analysis during sex inversion of Brycon orbignyanus reared in captivity.","authors":"Patricia Postingel Quirino, Maria Luiza Ribeiro Delgado, Luciane Gomes-Silva, Giovana Souza Branco, Renata Guimarães Moreira, Alexandre Ninhaus-Silveira, Rosicleire Veríssimo-Silveira","doi":"10.1007/s10695-025-01517-8","DOIUrl":"https://doi.org/10.1007/s10695-025-01517-8","url":null,"abstract":"Piracanjuba, Brycon orbignyanus, is a neotropical gonochoristic species of great ecological and commercial interest. When cultivated in captivity, it sexually differentiates into females, primary males, and secondary males that originate from females that undergo sex inversion. Therefore, the objective of this study was to verify the plasma profile of the androgens testosterone (T) and 11-ketotestosterone (11-KT) throughout the sex differentiation of B. orbignyanus maintained in captivity and ultrastructurally verifying the basement membrane in specimens that underwent natural sex inversion. For this purpose, monthly collections (n = 15/month) were carried out from February to December 2018, and samples were collected from two months to one year of age. The specimens were euthanized, and blood, gonads and liver were collected. The plasma was subjected to steroid analysis, and the androgens T and 11-KT were quantified. The gonadosomatic (GSI) and hepatosomatic (HSI) indices were measured to assess the reproductive stages of fish, and the gonads were subjected to histological and ultrastructural analyses. Among all the animals analyzed, undifferentiated specimens, females in early differentiation, intersex specimens, males in early differentiation, and females with functional ovaries were identified, all of which were considered immature, in addition to males with functional testes considered able to produce sperm. No significant differences were found in the plasma T profile, while males able to produce sperm and intersex individuals had higher plasma concentrations of 11-KT than the other groups analyzed. Thus, it is suggested that sex inversion in females in Brycon orbignyanus is driven by the action of the androgen 11-KT.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"104"},"PeriodicalIF":2.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144157635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0