Fish Physiology and Biochemistry最新文献

{"title":"Tissue-specific effects of stocking density on fatty acid composition and liver metabolism in rainbow trout (Oncorhynchus mykiss).","authors":"Zhao Li, Kang Dong, Yuling Xu, Yaxin Wang, Zhishuai Hou, Qinfeng Gao, Shuanglin Dong","doi":"10.1007/s10695-025-01555-2","DOIUrl":"https://doi.org/10.1007/s10695-025-01555-2","url":null,"abstract":"<p><p>Rainbow trout (Oncorhynchus mykiss), which is rich in high-quality protein and polyunsaturated fatty acids (PUFAs), is one of the most commercially important aquaculture species worldwide. The fatty acid profiles of trout, however, might be substantially influenced by different stocking densities. The present study aims to evaluate the effects of stocking density on the fatty acid composition and hepatic metabolomics of rainbow trout. Juvenile rainbow trout were reared for 84 days in circular tanks at 3 distinct levels of stocking density, i.e., low (group LD, 9.15 g/L), moderate (group MD, 13.65 g/L), and high (group HD, 27.31 g/L). The results showed that the levels of C22:6 n-3 (Docosahexaenoic acid, DHA) and total n-3 PUFAs in the dorsal muscles of the fish at group MD were significantly higher than those at groups LD and HD (p < 0.05). For ventral muscles, fish at group HD showed significantly decreased content of C16:1n-9 (palmitoleic acid) and n-3 PUFAs compared to those at groups LD and MD (p < 0.05). Hepatic fatty acid profiles showed significant reductions in monounsaturated fatty acids (MUFAs) for the fish at groups MD and HD compared to those at group LD (p < 0.05). Moreover, the concentrations of total n-6 PUFAs in the dorsal muscle were positively correlated with stocking density (R = 0.7357, p = 0.0238), while the levels of total n-3 PUFAs in the ventral muscle (R =  - 0.8478, p = 0.0039) and the levels of total MUFAs in the liver (R =  - 0.7837, p = 0.0101) showed negative correlations to the fish stocking densities. Metabolomics analyses further revealed that stocking density influenced the pathways related to glycerophospholipid metabolism, fatty acid degradation, and arachidonic acid metabolism. The results suggested that high stocking density altered fatty acid profiles and enhanced antioxidant and inflammatory responses in rainbow trout, providing valuable insights for optimizing aquaculture practices to improve the nutritional quality of farmed trout.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 5","pages":"164"},"PeriodicalIF":2.5,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145052596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and transcriptomic analyses reveal temperature-dependent regulation of stress response, protein synthesis and metabolic reprogramming in juvenile mandarin fish (Siniperca chuatsi) under simulated transport.","authors":"Jinghong He, Bingnan Li, Kexun Chen, Zhiqiang Deng, Sujia Wang, Bo Zhao, Yixiang He","doi":"10.1007/s10695-025-01581-0","DOIUrl":"https://doi.org/10.1007/s10695-025-01581-0","url":null,"abstract":"<p><p>With the rise of long-distance transport in aquaculture, temperature has become a key factor affecting juvenile fish survival and health. However, their molecular adaptation to transport temperature is not well understood. In this study, physiological and transcriptomic analyses were conducted to investigate the effects of transport temperatures on juvenile Siniperca chuatsi. With 25 °C non-transported fish as the control (C25), three transport temperature groups were established: transport at 15 °C (T15), transport at 25 °C (T25) and transport at 30 °C (T30). Comparative analyses were then performed between each transport group and the control (T15 vs. C25, T25 vs. C25 and T30 vs. C25). Liver damage became progressively more severe with increasing transport temperature, reaching its peak at T30 with pronounced edema and necrosis. Transcriptomic analysis identified over 5463 DEGs and three WGCNA modules significantly associated with temperature variation. Hub genes in the MEdarkseagreen4 module (T30) were enriched in lysosomal activity, calcium signaling and cytoskeletal regulation, indicating disrupted cellular homeostasis as a key driver of liver damage. At T15, the MEcyan module was enriched with upregulated hub genes for ribosome function and fatty acid metabolism, indicating boosted protein synthesis and energy use under low-temperature transport. In the T25, hub genes in the MEpalevioletred3 WGCNA module showed downregulation of oxidative phosphorylation and insulin signaling pathways, indicating a suppression of energy metabolism and growth signaling as part of a stress-adaptive strategy. These results deepen our understanding of the molecular mechanisms underlying temperature adaptation in eurythermal fish during simulated transport.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 5","pages":"163"},"PeriodicalIF":2.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and characterization of muscle cell line of spotted seabass (Lateolabrax maculatus).","authors":"Xiaoli Zheng, Jibin Lin, Ling Wang, Kai Song, Kangle Lu, Xueshan Li, Chunxiao Zhang","doi":"10.1007/s10695-025-01570-3","DOIUrl":"https://doi.org/10.1007/s10695-025-01570-3","url":null,"abstract":"<p><p>Spotted seabass (Lateolabrax maculatus) is an economically important fish species in China. In this study, the muscle of L. maculatus was used as a material, and the muscle cell line was successfully established using the tissue block method. The established muscle cell line exhibited vigorous growth and had been successfully passaged for more than 100 generations, maintaining stable polygonal cell morphology. The cell viability of L. maculatus muscle cell line exhibited a notable increase following a 24-h treatment with 30 <math><mi>μ</mi></math> M taurine, and the relative expression of myogenic regulatory factors myf5 and myf6 was significantly increased. Therefore, the muscle cell line of L. maculatus was successfully established and served as a valuable resource for conducting fundamental research in L. maculatus, providing essential materials for gene function analysis, cytogenetics, and nutritional metabolism mechanism in L. maculatus.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 5","pages":"161"},"PeriodicalIF":2.5,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral melatonin administration modulates apoptosis-related gene expression and counteracts early fertilization decline in overripe rainbow trout (Oncorhynchus mykiss) eggs.","authors":"Mahya Jabbari, Amirreza Abed-Elmdoust, Mohammad Meysam Salahi Ardekani, Kamran Rezaei Tavabe, Hamid Farahmand","doi":"10.1007/s10695-025-01577-w","DOIUrl":"https://doi.org/10.1007/s10695-025-01577-w","url":null,"abstract":"<p><p>Overripening of ovulated oocytes, often resulting from spawning delays in aquaculture, is triggered by apoptosis and significantly compromises early fertilization in fish. Targeting apoptosis pathways offers a promising approach to enhance reproductive outcomes, particularly given the controversial role of melatonin in apoptosis regulation, this study assessed the impact of oral melatonin supplementation at doses of 0, 50, and 200 mg/kg of dry feed on the expression of key apoptosis-related genes (Bcl-2, p53, caspase-9) and fertilization percentages in rainbow trout (Oncorhynchus mykiss) oocytes at two post-ovulation time points: immediately post-ovulation (day 0) and after 7 days of post-ovulatory ageing (overripening). Gene expression was quantified using real-time PCR, and fertilization percentages were evaluated to determine reproductive outcomes. Melatonin treatment notably upregulated the anti-apoptotic gene Bcl-2 and downregulated the pro-apoptotic genes p53 and caspase-9. While no significant differences were detected between the 50 and 200 mg doses for p53 and caspase-9 expression, the 200 mg dose demonstrated a more pronounced effect on improving fertilization percentages in overripened oocytes. In contrast, fertilization percentages declined sharply in the control group during overripening. Both melatonin dosages significantly improved fertilization percentages compared to the control, with higher doses showing greater effectiveness. These results suggest that melatonin alleviates the detrimental effects of oocyte overripening by modulating apoptosis pathways and enhancing fertilization outcomes. Melatonin supplementation may be a practical approach in aquaculture to extend oocyte viability and improve reproductive performance in delayed spawning scenarios.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 5","pages":"162"},"PeriodicalIF":2.5,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Zebrafish model for Iron Induced Neuroinflammation.","authors":"Siddhi Bagwe Parab, Ginpreet Kaur","doi":"10.1007/s10695-025-01575-y","DOIUrl":"https://doi.org/10.1007/s10695-025-01575-y","url":null,"abstract":"<p><p>Zebrafish models have been used to research Alzheimer's disease and other neurodegenerative disorders because of their similarities to the human genetic composition and behavior. Researchers have detected iron accumulation in the post-mortem brain sections of neurodegenerative disorder patients. Therefore, the development an animal model to simulate these clinical pathological findings is important. Iron is an important metal for maintaining homeostasis in the brain, depletion and accumulation of iron hamper neuronal development. Given the importance of iron overload in cognition impairment, this research aimed to develop an iron-induced zebrafish model of cognitive impairment. Zebrafish were subjected to ferrous sulfate (1.5 mg/L, 3 mg/L, and 6 mg/L) for 28 days. The behavioral parameters (Y-maze, novel tank test), oxidative stress parameters (MDA, GSH, and catalase), acetylcholinesterase (AChE) levels, iron levels, and interleukin-1β (IL-1β) levels in brain homogenate were assessed. The behavioral and locomotor responses, specifically in the zebrafish treated with iron for 28 days, suggest an increase in the loss of spatial memory and anxiety. Reactive oxygen species in the brain significantly increased (p < 0.001) with the increase in concentrations of iron. Brain tissue iron content and IL-1β significantly increased (p < 0.001) in the brain homogenate of the zebrafish. This model will aid in the screening of therapeutic compounds to accelerate drug discovery in the field of neurodegenerative diseases.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 5","pages":"160"},"PeriodicalIF":2.5,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigenetic horizons in aquaculture: unlocking sustainable fish production.","authors":"Khalid Hussain, Syed Makhdoom Hussain, Shafaqat Ali, Ameer Fawad Zahoor, Ebru Yilmaz, Abdulrahman Alasmari, Muhammad Munir, Muhammad Zubair-Ul-Hassan Arsalan, Adan Naeem","doi":"10.1007/s10695-025-01564-1","DOIUrl":"https://doi.org/10.1007/s10695-025-01564-1","url":null,"abstract":"<p><p>Epigenetics has a profound impact on fish nutrition and aquaculture by regulating gene expression, physiological traits, and growth without altering the underlying DNA sequence. The changes, particularly DNA methylation, can be passed down through generations, enhancing productivity and disease resistance. External factors like temperature, stress, nutrition and illness exposure can also influence epigenetic changes, affecting protein, omega-3 fatty acids, and probiotics. DNA methylation and dietary factors also enhance resilience, promoting fish health and reducing antibiotic reliance. Unlocking innovative tactics that promote ecological sustainability, economic viability, and food security requires a deeper comprehension of the epigenetic landscape in aquatic species. Epigenetic insights represent a frontier for advancing precision aquaculture and ensuring the long-term resilience of fish production systems. Combining epigenetic knowledge with conventional breeding methods can lead to faster development of fish lines with improved growth rates, disease resistance, and optimal feed conversion. Understanding the epigenetic processes underlying fish nutrition can lead to sustainable aquaculture methods, increased productivity, and improved overall fish health. Dynamically shaped by environmental and nutritional factors, DNA methylation and histone modification improve performance and adaptability. Understanding and applying histone modifications can greatly enhance the resilience and sustainability of aquaculture practices.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 5","pages":"159"},"PeriodicalIF":2.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of nano-selenium on the histopathological changes and gene expression of rainbow trout (Oncorhynchus mykiss) spleen under heat stress.","authors":"Junhao Lu, Zhe Liu, Jiahui Zhang, Lanlan Li, Guiyan Zhao, Yucai Pan, Jinqiang Quan, Guolin Song","doi":"10.1007/s10695-025-01576-x","DOIUrl":"https://doi.org/10.1007/s10695-025-01576-x","url":null,"abstract":"<p><p>Rainbow trout(Oncorhynchus mykiss) is a typical cold-water fish often threatened by high summer temperatures. Nano-selenium as a feed additive can improve the antioxidant capacity of the body and relieve stress. In this study, different levels of nano-selenium (0, 5 and 10 mg/kg) were added to the feed of rainbow trout to determine the changes in spleen structure and expression of related genes in rainbow trout at the proper temperature (18℃) and heat stress temperature (24℃). The results showed that the addition of 5 mg/kg nano-selenium had a more obvious protective effect on rainbow trout spleen. The addition of nano-selenium improved the antioxidant capacity and immunity of rainbow trout spleen at 18 °C. After heat stress, the addition of 5 mg/kg of nano-selenium significantly increased the expression levels of spleen heat shock protein genes, such as HSP10, HSP47, HSP70, HSP90, antioxidant-related genes, such as glutathione peroxidase (GPx), thioredoxin reductase (Trx), superoxide dismutase (SOD1), catalase (CAT) and immune-related genes, such as tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β), Immunoglobulin M (IgM), Complement Component 3 (C3), resulting in the improvement of antioxidant capacity and immunity, and the protection of the spleen to alleviate the damages caused by heat stress. This study provides a theoretical basis for the development of anti-heat stress feeds for rainbow trout.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 5","pages":"158"},"PeriodicalIF":2.5,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature and photoperiod stress in zebrafish larvae: impacts on development, gene regulation and PGC migration.","authors":"María Santos-Villadangos, Alba Sellés-Egea, Vanesa Robles, David G Valcarce","doi":"10.1007/s10695-025-01568-x","DOIUrl":"10.1007/s10695-025-01568-x","url":null,"abstract":"<p><p>Early life stress is key in development and behaviour and may have long-term effects, as it represents a window of increased vulnerability. In our study we evaluated two stressors, temperature and photoperiod -alone and combined-, in zebrafish (Danio rerio) larvae up to 120 h post-fertilisation. The objective of this study was to investigate the effects of non-lethal environmental stressors and their potential synergistic effects until completion of organogenesis integrating molecular, cellular and behavioural analyses. Larvae were subjected to 4 experimental conditions: \"control\" (C) (14 h light: 10 h darkness; T = 27 ± 1 °C); \"heat shock\" (HS) (14 h light: 10 h darkness; T = 34 °C); \"darkness\" (D) (24 h darkness; T = 27 ± 1 °C) and \"stressed\" (S⁺) (24 h darkness; T = 34 °C). Our results confirmed phenotypic, behavioural and molecular alterations. The S⁺ larvae showed a lower survival curve, lower regeneration, a higher number of malformations, predominantly those related to the swim bladder, lower motor activity and a dysregulation of miR29a and clocka, hspa9, hspa5 and cxcr4b genes. The results revealed a similar number of Ddx4⁺ cells between groups but non-canonical migration patterns in the S⁺ group. This integrated approach offers new insights into the molecular mechanisms underlying the stress response during embryogenesis and provides a new perspective on the interaction between temperature and light on early-stage development.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 5","pages":"156"},"PeriodicalIF":2.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12408740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary supplementation with the cyanobacterium Synechococcus elongatus PCC 7942 on the brain transcriptome of zebrafish (Danio rerio).","authors":"Mirna Leandra Enriquez Reyes, Andrea Idelette Hernandez, Raíza Dos Santos Azevedo, Beatriz Xavier Figueiredo, Isaac Dos Santos Flores, Tony Silveira, Iuri Salim Abou Anni, Lucielen Oliveira Dos Santos, Bruno Roswag Machado, Bruna Felix Nornberg, Luis Fernando Marins","doi":"10.1007/s10695-025-01573-0","DOIUrl":"10.1007/s10695-025-01573-0","url":null,"abstract":"<p><p>The application of cyanobacteria as sustainable feed additives in aquaculture has gained increasing attention due to their nutritional and functional properties. In previous studies, we demonstrated that dietary supplementation with Synechococcus elongatus PCC 7942 improved hepatic homeostasis and modulated the intestinal microbiota of zebrafish (Danio rerio), without adverse effects on intestinal morphology. Building upon these findings, the present study aimed to expand our understanding of the transcriptomic responses in the zebrafish brain to dietary supplementation with S. elongatus. The cyanobacterial biomass presented a proximate composition of 45.19% protein, 21.34% lipids, 10.08% carbohydrates, and 8.01% ash, along with a fatty acid profile rich in palmitic acid (39.96%) and linoleic acid (12.25%). After 35 days of feeding, fish receiving the supplemented diet showed higher daily feed intake and condition factor, while other growth parameters did not differ significantly from the control group. Transcriptomic analysis of the brain revealed differential expression of 15 genes grouped into five functional categories: energy metabolism, calcium homeostasis, neuroplasticity, oxidative stress response, and metabolic regulation. Overexpression of genes such as ldhbb, gapdh, and tsc1a suggests that S. elongatus modulates brain metabolism through enhanced glycolysis and activation of protective cellular pathways. The findings indicate that S. elongatus PCC 7942 exerts neuroprotective and metabolic effects, supporting its potential as a novel dietary supplement in aquaculture.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 5","pages":"157"},"PeriodicalIF":2.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of thymol application for anaesthesia of adult zebrafish.","authors":"Luís Félix, Sandra M Monteiro, Carlos Venâncio","doi":"10.1007/s10695-025-01574-z","DOIUrl":"10.1007/s10695-025-01574-z","url":null,"abstract":"<p><p>The use of anaesthetics has become extremely important in promoting responsible welfare practices in aquaculture and research. However, adverse reactions and side effects have been described for commonly used anaesthetics such as MS-222 and eugenol. This study examined thymol as a new anaesthetic for adult zebrafish (Danio rerio), a species for which no previous thymol anaesthesia studies have been reported. Concentration-response tests (25-200 mg/L) were conducted using 10 individual animals per concentration, along with assessments of cardiorespiratory, locomotion, and aversive-related effects. Concentrations below 25 mg/L did not induce anaesthetic effects while 50 and 75 mg/L were most effective, inducing deep anaesthesia with average induction times of around 300 s and recovery times of between 400 and 500 s. Induction times at these concentrations were comparable to those observed with MS-222 and eugenol; however, recovery periods were longer. No mortality was observed at these concentrations, although transient cardiorespiratory depression was noted. Video tracking analyses revealed no significant behavioural changes at the lowest concentrations. In contrast, concentrations above 150 mg/L showed narrow behavioural safety margins and induced aversive responses, rendering them unsuitable for fish aesthesia. Based on these findings, a short 10-min exposure to 50 mg/L thymol resulted in cortisol levels comparable to eugenol and lower than in the control group. These findings support the use of 50 mg/L thymol as an effective and welfare-conscious anaesthetic for adult zebrafish. However, further research is recommended to elucidate thymol's physiological effects and its mechanism of action in this species.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 5","pages":"155"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12402031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}