Fish Physiology and Biochemistry最新文献_第2页

Mutagenic and cellular integrity disruptions in freshwater food fish exposed to mercuric chloride contamination. 受氯化汞污染的淡水食用鱼的致突变性和细胞完整性破坏。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-07-21 DOI: 10.1007/s10695-025-01536-5

Manoj Kumar, Shefalee Singh, Anamika Jain, Vidyanand Tiwari, Yashika Awasthi, Kamlesh K Yadav, Shraddha Dwivedi, Sunil P Trivedi

{"title":"Mutagenic and cellular integrity disruptions in freshwater food fish exposed to mercuric chloride contamination.","authors":"Manoj Kumar, Shefalee Singh, Anamika Jain, Vidyanand Tiwari, Yashika Awasthi, Kamlesh K Yadav, Shraddha Dwivedi, Sunil P Trivedi","doi":"10.1007/s10695-025-01536-5","DOIUrl":"https://doi.org/10.1007/s10695-025-01536-5","url":null,"abstract":"Mercuric chloride (HgCl2) contamination in aquatic environments poses significant threats to aquatic life, disrupting cellular functions and overall organism health. The impact of sublethal concentrations of HgCl2 on the freshwater food fish Channa punctatus was examined in this 60-day study, with an emphasis on cellular and molecular reactions, specifically DNA damage and the related signaling pathways. A total of 135 fish were distributed among 3 groups and exposed to sublethal concentrations of HgCl2 at 0.0 (group I), 0.039 (group II), and 0.078 mg L-1 (group III). Elevated DNA damage was observed in fish exposed to HgCl2, as quantified by the comet assay, with the highest DNA tail length in the group exposed to the highest HgCl2 concentration. A significant (p < 0.05) increase in apoptotic cells (AC), in a dose-dependent manner was also noted. Upregulation of mRNA transcripts for Ataxia telangiectasia mutated (ATM), checkpoint kinase 2 (CHK2), tumor protein (P53), and (P21) indicated enhanced apoptotic and cell cycle arrest mechanisms. The study also highlighted elevated activation of Mitogen-Activated Protein Kinase (MAPK) pathways, particularly p38 MAPK, in the liver and kidney tissues, implicating their role in mediating stress responses and inflammation. Histological analysis and SEM analysis corroborated these molecular findings, revealing significant tissue damage, including hepatic inflammation and renal tubular disorientation. Regression analysis showed strong correlations between various molecular markers in liver tissues, while kidney tissues exhibited variable responses, suggesting different regulatory mechanisms. These results underscore the comprehensive impact of HgCl2 on cellular integrity and stress response pathways, providing novel insights into the tissue-specific effects of heavy metal toxicity.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"125"},"PeriodicalIF":2.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential antioxidant effect of creatine on the functionality of intratesticular spermatozoa of coho salmon (Oncorhynchus kisutch). 肌酸对银鲑睾丸内精子功能的潜在抗氧化作用。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-07-18 DOI: 10.1007/s10695-025-01535-6

Paola Niedmann, Osvaldo Merino, Sebastián Ávila, Wellison Amorim Pereira, Jorge G Farías, Ricardo Pinheiro S Oliveira, Alejandro Villasante, Iván Valdebenito Isler, Elías Figueroa Villalobos

{"title":"Potential antioxidant effect of creatine on the functionality of intratesticular spermatozoa of coho salmon (Oncorhynchus kisutch).","authors":"Paola Niedmann, Osvaldo Merino, Sebastián Ávila, Wellison Amorim Pereira, Jorge G Farías, Ricardo Pinheiro S Oliveira, Alejandro Villasante, Iván Valdebenito Isler, Elías Figueroa Villalobos","doi":"10.1007/s10695-025-01535-6","DOIUrl":"https://doi.org/10.1007/s10695-025-01535-6","url":null,"abstract":"Quality control of intratesticular sperm samples obtained through testicular maceration faces significant challenges in salmon aquaculture. Mechanical damage during maceration and tissue contamination induces oxidative stress owing to reactive oxygen species (ROS). Due to the ability of antioxidants to reduce oxidative stress, this study evaluated the antioxidant effect of creatine on intratesticular sperm functionality and quality in fifteen males of coho salmon (Oncorhynchus kisutch). Two experiments were conducted under short-term storage (4 °C). In experiment 1, four creatine concentrations were tested: group 1 (0 mM, control), group 2 (2.5 mM), group 3 (5.0 mM), and group 4 (7.5 mM), with measurements at 0 h and 24 h. Experiment 2 involved lower concentrations: group 1 (0 mM), group 2 (0.1 mM), group 3 (0.2 mM), and group 4 (0.4 mM), assessed after 1-h incubation. Both experiments evaluated sperm kinetics (motility, curvilinear velocity [VCL], straight-line velocity [VSL], average path velocity [VAP]) using Computer-Assisted Sperm Analysis (CASA) and sperm functionality (membrane integrity, mitochondrial potential, anion superoxide levels, and DNA fragmentation) via flow cytometry. In experiment 1, group 2 showed higher motility, and group 4 displayed reduced mitochondrial ROS at 0 h. In experiment 2, group 4 had significantly lower cytoplasmic and mitochondrial ROS levels, indicating enhanced antioxidant protection. Overall, creatine monohydrate demonstrated antioxidant properties, reducing oxidative stress, lowering ROS levels and DNA fragmentation in group 4 (0.4 mM). These findings highlight creatine's potential to enhance sperm quality in aquaculture, particularly for short-term storage of intratesticular sperm.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"123"},"PeriodicalIF":2.5,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of long-term exposure to single and mixed mineral ions related to water hardness on Nile tilapia (Oreochromis Niloticus). 长期暴露于单一和混合矿物离子对尼罗罗非鱼（Oreochromis Niloticus）水硬度的影响。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-07-17 DOI: 10.1007/s10695-025-01532-9

Eman I Hassanen, Wafaa A Mohamed, Hanan S Khalefa, Ghada E Ali, Mahmoud A Mahmoud

{"title":"The effect of long-term exposure to single and mixed mineral ions related to water hardness on Nile tilapia (Oreochromis Niloticus).","authors":"Eman I Hassanen, Wafaa A Mohamed, Hanan S Khalefa, Ghada E Ali, Mahmoud A Mahmoud","doi":"10.1007/s10695-025-01532-9","DOIUrl":"10.1007/s10695-025-01532-9","url":null,"abstract":"The current study aimed to evaluate the harmful effects of single and mixed minerals involved in water hardness on Nile tilapia (Oreochromis niloticus). Seventy-five fish were randomly assigned to five groups (n = 15), each consisting of three replicates (n = 5), and subjected to the following treatments for two months: standard fishery water, Ca (186 mg/L CaCl₂), Mg (40 mg/L MgCl₂), Na (25 g/L NaHCO₃), a mixture of MgCl₂, CaCl₂, and NaHCO₃ at the same protocol dosing to adjust water hardness at 300 mg/L. The results reported that fish exposed to Ca, Na, and Mix salts showed a significant decline in growth performance, serum antioxidant levels, and protein profile along with a significant rise in the enzymatic activity of ALT, AST, urea, creatinine, and MDA levels (P ≤ 0.05). Exposure of fish to either single or mixed ions caused histopathological changes in the gills, liver, kidney, spleen, and brain. However, the nature, distribution, and the degree of severity of histopathological lesions vary in the experimental groups. The group exposed to mixed salts had the highest scores in all the pathological parameters, whereas the Mg group recorded the lowest scores. Moreover, the Ca group exhibited the highest scores in the liver and kidney, while the Na group showed the highest scores in the gills, spleen, and kidney.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"122"},"PeriodicalIF":2.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12271268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiology and morphology of clonal Atlantic salmon-influence of incubation temperature, ploidy, and zygosity. 无性系大西洋鲑的生理和形态——孵育温度、倍性和合子性的影响。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-07-17 DOI: 10.1007/s10695-025-01533-8

Malthe Hvas, Fletcher Warren-Myers, Ida B Johansen, Per Gunnar Fjelldal, Tom Johnny Hansen

{"title":"Physiology and morphology of clonal Atlantic salmon-influence of incubation temperature, ploidy, and zygosity.","authors":"Malthe Hvas, Fletcher Warren-Myers, Ida B Johansen, Per Gunnar Fjelldal, Tom Johnny Hansen","doi":"10.1007/s10695-025-01533-8","DOIUrl":"10.1007/s10695-025-01533-8","url":null,"abstract":"Isogenic (clonal) fish lines are useful experimental models to study effects of environment versus genetics on phenotypic traits, as they can be maintained for generations without change, providing advantages over outbred groups prone to generational change and higher variation. Here we performed experiments on isogenic Atlantic salmon groups that were either heterozygous diploid, homozygous diploid, triploid, or heterozygous diploid incubated at 4 °C instead of 8 °C. We measured metabolic rates, stress response, and hypoxia tolerance to assess whole-animal performance traits. Then we measured the morphology of hearts and otoliths since both are known to be influenced by environmental history. Isogenic, ploidy, and zygosity statuses were confirmed from microsatellite markers. Embryonic development is affected by temperature, hence the 4 °C incubation group was tested 9 months later when it had reached an equivalent size as the other groups. Curiously, a bimodal size distribution emerged in this group. Physiological traits were similar between groups apart from higher standard metabolic rates in the 4 °C incubated fish. Each group had distinct heart morphologies where fish with a slower growth history resembled wild-phenotypes while homozygous fish had the most deviating hearts. Proportions of vaterite deposition in otoliths showed high individual variation and did not differ between groups. Lower coefficients of variation within groups were found when compared to outbred fish, but this was not consistent for all traits assessed. As such, substantial phenotypic variation in physiology and morphology was still observed in isogenic Atlantic salmon, which can be ascribed to random environmental factors.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"121"},"PeriodicalIF":2.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12270969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pumpkin peel (Cucurbita pepo)-fortified diet mitigates trace element mixture (mercury and zinc)-induced hepato-renal dysfunction, immunotoxicity, splenic gene disruption, and histopathological alterations in Oreochromis niloticus. 南瓜皮强化饮食减轻微量元素混合物（汞和锌）引起的尼罗褐虾肝肾功能障碍、免疫毒性、脾基因破坏和组织病理学改变。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-07-16 DOI: 10.1007/s10695-025-01534-7

Rowida E Ibrahim, Afaf N Abdel Rahman, Mohammed S Sobh, Abdallah Tageldein Mansour, Ahmed S Alsaqufi, Yousef Alkhamis, Hesham A Hassanien, Ahmed Abbas, Tarek Khamis, Abdelrahman S Talab

{"title":"Pumpkin peel (Cucurbita pepo)-fortified diet mitigates trace element mixture (mercury and zinc)-induced hepato-renal dysfunction, immunotoxicity, splenic gene disruption, and histopathological alterations in Oreochromis niloticus.","authors":"Rowida E Ibrahim, Afaf N Abdel Rahman, Mohammed S Sobh, Abdallah Tageldein Mansour, Ahmed S Alsaqufi, Yousef Alkhamis, Hesham A Hassanien, Ahmed Abbas, Tarek Khamis, Abdelrahman S Talab","doi":"10.1007/s10695-025-01534-7","DOIUrl":"https://doi.org/10.1007/s10695-025-01534-7","url":null,"abstract":"Waterborne trace element mixture (TEM) negatively affects the physiological performance of fish and health status in recent years. Pumpkin peels (Cucurbita pepo, CPP; 12 g/kg diet) as a target of polyphenol substances was evaluated against TEM [mercury (Hg) and zinc (Zn)] exposure in Oreochromis niloticus (Nile tilapia) in this investigation. Comprehensive biochemical, molecular, and histological assays were evaluated. A total of 160 fish (28.50 ± 1.33 g) were assigned into four groups for 60 days. The control and CPP groups were fed basal and CPP diets without trace element exposure, respectively. The TEM and CPP + TEM groups were exposed to TEM (Hg at 0.05 mg/L and Zn at 7.00 mg/L) and given the basal and CPP diets, respectively. Fish mortality (27.50%) and hepato-renal variables increased significantly in TEM fish. The TEM group experienced lower serum complement 3 activity, lysozyme activity, and nitric oxide levels. Furthermore, downregulation of immune genes (interleukin-10, heme oxygenase-1, and Kelch-like ECH-associated protein-1) expression and upregulation of tumor necrosis factor-α gene expression were observed in the TEM group. Several pathological lesions were observed in the liver, kidney, and splenic tissues of the TEM fish. Fortification with CPP in the diet decreased the mortality rate and modulated hepato-renal markers in the TEM group. The humoral and splenic immune responses of the TEM fish and histological architecture were enhanced with the CPP-supplemented diet. Overall, the dietary intervention of CPP (12 g/kg diet) is recommended to overcome TEM negative effects on the physio-biochemical and immunological responses of Nile tilapia.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"120"},"PeriodicalIF":2.5,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative evaluation of antioxidant status, stress, and hepatorenal biomarkers in Nile tilapia (Oreochromis niloticus) cultured in In-Pond raceway and traditional aquaculture systems. 尼罗罗非鱼（Oreochromis niloticus）在池内和传统养殖系统中抗氧化状态、应激和肝肾生物标志物的比较评价

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-07-04 DOI: 10.1007/s10695-025-01531-w

Salah M Aly, Osama A Abd Allah, Noha I ElBanna, Nahla S Abdel-Naeim, Noha S Abdelnaeim, Mohamed Fathi

{"title":"Comparative evaluation of antioxidant status, stress, and hepatorenal biomarkers in Nile tilapia (Oreochromis niloticus) cultured in In-Pond raceway and traditional aquaculture systems.","authors":"Salah M Aly, Osama A Abd Allah, Noha I ElBanna, Nahla S Abdel-Naeim, Noha S Abdelnaeim, Mohamed Fathi","doi":"10.1007/s10695-025-01531-w","DOIUrl":"10.1007/s10695-025-01531-w","url":null,"abstract":"The in-pond raceway system (IPRS), is an intensive aquaculture technology that has gained attention in recent years. This study evaluated the comparative effects of In-Pond Raceway Systems (IPRS) and traditional aquaculture systems on oxidative status, stress indices, and biomarkers of liver and kidney function, as well as proteinogram in Nile tilapia (Oreochromis niloticus) over 2, 4, and 6 months. The results revealed that serum total antioxidative capacity (TAC) level were significantly lower in the IPRS group compared to the traditional system at all time points (p < 0.01 at 2 months, p < 0.001 at 4 and 6 months), indicating oxidative stress in the IPRS. Stress markers such as cortisol and glucose levels were significantly elevated in the IPRS group at 4 and 6 months (p < 0.05 and p < 0.001, respectively), while triglycerides and cholesterol levels were consistently higher in the IPRS group across all time points (p < 0.001 for triglycerides, p < 0.001 at 2 months and p < 0.01 at 4 and 6 months for cholesterol). Liver function biomarkers, including alanine aminotransferase (ALT) and aspartate aminotransferase (AST), showed no significant differences between the two systems, except for a transient increase in ALT at 4 months in the IPRS group (p < 0.05). These findings underscore the need for optimized IPRS management strategies, particularly adjusted stocking densities, to mitigate physiological stress and ensure sustainable aquaculture. The traditional system demonstrated lower stress impacts, promoting better health and metabolic stability, offering valuable insights for balanced aquaculture practices.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"119"},"PeriodicalIF":2.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of homochromatic lights on the physiological responses and morphochromatic traits of scarlet badis, Dario dario (Hamilton, 1822). 同色光对鲜红女花生理反应和形态色性状的影响，Dario Dario （Hamilton, 1822）。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-07-01 DOI: 10.1007/s10695-025-01523-w

N Siva, Gouranga Biswas, Sweta Pradhan, Arunashri A, Paramita B Sawant

{"title":"Effects of homochromatic lights on the physiological responses and morphochromatic traits of scarlet badis, Dario dario (Hamilton, 1822).","authors":"N Siva, Gouranga Biswas, Sweta Pradhan, Arunashri A, Paramita B Sawant","doi":"10.1007/s10695-025-01523-w","DOIUrl":"10.1007/s10695-025-01523-w","url":null,"abstract":"Aquarium fish keeping ranks as the world's second most popular hobby, with a global trade value of 18-20 billion USD annually. Despite its popularity, aquarium lighting is often used for aesthetics without considering its biological effects on fish. This study examined how homochromatic lights affect growth, feed utilization, survival, stress markers, hormone levels, and skin pigmentation in the ornamental fish Dario dario (scarlet badis). Ten male fish (92.79 ± 4.33 mg) were stocked per tank and exposed to six light treatments: red light (RL), blue light (BL), green light (GL), white light (WL), ambient light (AL), and dark (D) for 30 days under a 12:12-h (light:dark) photoperiod. Among treatments, GL significantly enhanced growth, feed efficiency, and survival. Stress biomarkers, namely superoxide dismutase activity, catalase activity, glucose, and cholesterol contents were significantly lower (P < 0.01) under GL, WL, and AL. Fish under GL also had elevated 11-keto testosterone and reduced cortisol levels (P < 0.01), indicating hormonal balance. Visibly, GL-exposed fish displayed more vibrant red bands, confirmed through skin chromaticity analysis and microscopic observation of pigment dispersion in chromatophores. In summary, green light created a favourable environment for D. dario, improving growth, stress reduction, and ornamental value.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"118"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin receptor-mediated signaling pathways drive time-dependent physiological recovery from freezing in Perccottus glenii. 褪黑激素受体介导的信号通路驱动棉铃虫冷冻后的时间依赖性生理恢复。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-06-26 DOI: 10.1007/s10695-025-01516-9

Zhaoyang Ning, Cunrun Ye, Yingqiao Chen, Jiajun Zhou, Xiaoyu Zhao, Ye Huang, Zhitao Liu, Weijie Mu

{"title":"Melatonin receptor-mediated signaling pathways drive time-dependent physiological recovery from freezing in Perccottus glenii.","authors":"Zhaoyang Ning, Cunrun Ye, Yingqiao Chen, Jiajun Zhou, Xiaoyu Zhao, Ye Huang, Zhitao Liu, Weijie Mu","doi":"10.1007/s10695-025-01516-9","DOIUrl":"https://doi.org/10.1007/s10695-025-01516-9","url":null,"abstract":"Freeze tolerance in fish has been associated with various physiological adaptations; however, the role of the melatonin (MT) system in fish freeze recovery remains poorly understood. This study investigates the role of MT and its receptors in the recovery phase from hibernation in the fish species Perccottus glenii. The results showed that during the recovery phase, MT content in untreated P. glenii increased gradually, with significantly higher levels at 12 h than at 4 h. MT injection facilitated the recovery of P. glenii, as evidenced by significant differences in hibernation recovery behaviors between injected and non-injected fish. In the liver during recovery, the mRNA levels of AANAT and HIOMT, key enzymes for MT secretion, were higher at 12 h. The expressions of MT receptors MTNR1A, MTNR1B, and MTNR1C were also upregulated at 12 h. In the liver, the expressions of PKC and ERK increased significantly at 12 h, suggesting MT role through the receptor pathway. Experiments with primary hepatocytes indicated that the MT receptor promoted PKC expression. In the liver during recovery, ERK and PKC protein levels were higher at 12 h than at 4 h, while PKA was lower. Various expression changes were observed for PKG, NF-κB, AKT, CAMK1, and CREB in different treatment groups. There were significant differences in the mRNA expressions of PKG, AKT, and CAMK1 between 12 and 4 h recovery cells and in CREB levels among the control, 4 h, and 12 h groups. The findings suggest that MT is involved in the hibernation recovery process, and its receptor-mediated signaling pathways play important roles, with mechanisms that may be time-dependent. The present study highlights the importance of the MT system in fish freeze recovery and offers a potential target for further research on improving fish survival in cold conditions.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"116"},"PeriodicalIF":2.5,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of urea nitrogen fertilizer on the physiology, behavior, and histology of juvenile rainbow trout. 尿素氮肥对虹鳟鱼幼鱼生理、行为和组织学的影响。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-06-26 DOI: 10.1007/s10695-025-01528-5

Tomas Makaras, Julija Razumienė, Vidutė Gurevičienė, Gintarė Sauliutė, Nataliia Matviienko, Mykhailo Kozij, Milda Stankevičiūtė

{"title":"Impact of urea nitrogen fertilizer on the physiology, behavior, and histology of juvenile rainbow trout.","authors":"Tomas Makaras, Julija Razumienė, Vidutė Gurevičienė, Gintarė Sauliutė, Nataliia Matviienko, Mykhailo Kozij, Milda Stankevičiūtė","doi":"10.1007/s10695-025-01528-5","DOIUrl":"https://doi.org/10.1007/s10695-025-01528-5","url":null,"abstract":"Excessive use of nitrogen (N) fertilizers can induce metabolic alterations in fish by disrupting nitrogen metabolism and depleting energy reserves needed to maintain nitrogen homeostasis. This study evaluated the acute (96-h LC50) and prolonged (14-day) effects of urea nitrogen fertilizer (5 g L⁻1 was determined to be the lowest tested concentration with no observable effects) on the physiological, histological, and behavioral responses of rainbow trout (Oncorhynchus mykiss) juveniles. Significant increases in internal urea levels were observed in the blood, gills, and kidneys of exposed fish, alongside decreased glucose levels in blood and renal tissues. The kidneys showed time-dependent changes in urea levels, while circulating glucose levels varied across tissues, with the kidneys exhibiting the most pronounced response. These variations reflect physiological compensatory mechanisms in response to urea-N exposure. A 14-day exposure to urea caused both functional and structural changes in the gills, liver, and kidneys. Increased swimming activity was also noted, persisting throughout the exposure period. Water urea measurements throughout 14-days period were significant but less informative for identifying patterns associated with urea level changes. Further research is needed to investigate the effects of other nitrogen compounds, particularly those associated with eutrophication, and to optimize the use of these biomarkers for indicating metabolic consequences across various biological matrices.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"115"},"PeriodicalIF":2.5,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of galanin in reproduction of male tilapia, Oreochromis mossambicus. 甘丙肽在雄性罗非鱼繁殖中的作用。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-06-26 DOI: 10.1007/s10695-025-01527-6

Ashwini Biradar, C B Ganesh

{"title":"Involvement of galanin in reproduction of male tilapia, Oreochromis mossambicus.","authors":"Ashwini Biradar, C B Ganesh","doi":"10.1007/s10695-025-01527-6","DOIUrl":"https://doi.org/10.1007/s10695-025-01527-6","url":null,"abstract":"Galanin(GAL) is recognised for its involvement in various physiological, behavioural, and neuroendocrine functions in vertebrates. Yet, the impact of this peptide on spermatogenesis in fish remains understudied. In this investigation, the administration of 5 µg of GAL receptor agonist (GAL-A) to the tilapia Oreochromis mossambicus did not significantly alter the numbers of different germ cells except for a significant rise in the number of late spermatids compared to the control group. By contrast, treatment with 20 µg of GAL-A caused a significant rise in all germ cell counts and tubule and lumen areas and a significant reduction in the area of interstitium relative to the control group. The numbers of spermatogonia-A, primary and secondary spermatocytes, and early spermatids showed a strong positive association with regard to their respective cyst size. Furthermore, fish administered 20 µg of GAL-A exhibited significant increases in the percentage of GnRH-immunoreactive fibres within the nucleus lateralis tuberis and proximal pars distalis of the pituitary gland, concomitant with significantly higher serum levels of LH, and the intensity of androgen receptor protein expression in the testis when compared to control groups. The in vitro experimental results revealed a significantly increased level of 11-KT in the testis treated with 30 or 300 µM of GAL-A when compared to the control group. Together, these results demonstrate for the first time that GAL-A enhances spermatogenesis and steroidogenesis in the testis. GAL-A may influence these effects through the hypothalamic-pituitary axis and directly at the testicular level in teleosts.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 4","pages":"117"},"PeriodicalIF":2.5,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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