Fish Physiology and Biochemistry最新文献_第3页

Curcumin's role in reshaping the redox dynamics of fish kidneys: NRF2 activation as a strategy against copper-induced nephropathy. 姜黄素在重塑鱼类肾脏氧化还原动力学中的作用：NRF2激活作为一种对抗铜诱导肾病的策略。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-05-07 DOI: 10.1007/s10695-025-01506-x

Abha Trivedi, Vaishnavi Saxena, Jumman Bakhasha, Neeti Arya, Kamlesh K Yadav, Alok Srivastava, Sunil P Trivedi, Mahdi Banaee, Mentor Sopjani, Caterina Faggio

{"title":"Curcumin's role in reshaping the redox dynamics of fish kidneys: NRF2 activation as a strategy against copper-induced nephropathy.","authors":"Abha Trivedi, Vaishnavi Saxena, Jumman Bakhasha, Neeti Arya, Kamlesh K Yadav, Alok Srivastava, Sunil P Trivedi, Mahdi Banaee, Mentor Sopjani, Caterina Faggio","doi":"10.1007/s10695-025-01506-x","DOIUrl":"https://doi.org/10.1007/s10695-025-01506-x","url":null,"abstract":"From essential to harmful, excess copper compromises aquatic vitality. Curcumin, a potent antioxidant bioactive, counteracts heavy metal toxicity. This study examines its role in modulating the NRF2-KEAP1 pathway to boost antioxidant defenses and mitigate apoptosis in kidneys of Channa punctatus exposed to environmentally relevant Copper concentrations (ERCC). 180 fully habituated fish were categorized into six groups: Group 1 served as control, Group 2 was treated with 3 mg/L Curcumin, Group 3 was exposed to ERCC (0.85 mg/L Copper), while Group 4, Group 5 and Group 6 received co-exposure to ERCC along with the escalating Curcumin concentrations of 1 mg/L, 2 mg/L, and 3 mg/L, respectively, over periods of 15, 30, 45, 60, and 75 days. Biochemical assays were conducted to evaluate oxidative stress markers (Reactive oxygen species, reduced glutathione, glutathione peroxidase, and lipid peroxidation), kidney damage indicators (creatinine), and genotoxicity (micronuclei). Additionally, transcriptional profiling assessed mRNA levels of apoptosis-related factors (p53, bax, apaf1, cas9, cas3 and bcl2), while histopathological examinations revealed changes in renal architecture. Molecular docking analysis confirmed Curcumin's strong binding affinity to KEAP1, providing insights into its role in activating the NRF2-KEAP1 pathway. The results indicated that Curcumin significantly (p < 0.05) reduced Copper-induced oxidative stress, improved antioxidant defenses, suppressed genotoxicity, modulated apoptosis, and maintained renal tissue integrity. These findings validate curcumin's potential in effectively combating copper toxicity in aquaculture, paving the way for enhanced fish health and improved food safety.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"94"},"PeriodicalIF":2.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic hypoxia induces alternative splicing of transcripts in the goldfish brain. 慢性缺氧诱导金鱼脑内转录本的选择性剪接。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-05-07 DOI: 10.1007/s10695-025-01505-y

Catherine Cao, Niepukolie Nipu, William Johnston, Sally Adil, Lai Wei, Jan A Mennigen

{"title":"Chronic hypoxia induces alternative splicing of transcripts in the goldfish brain.","authors":"Catherine Cao, Niepukolie Nipu, William Johnston, Sally Adil, Lai Wei, Jan A Mennigen","doi":"10.1007/s10695-025-01505-y","DOIUrl":"https://doi.org/10.1007/s10695-025-01505-y","url":null,"abstract":"Several species evolved mechanisms to tolerate periods of severe environmental hypoxia and anoxia. Among them, goldfish are unique as they do not enter a comatose state under such conditions. Taking advantage of the recently published and annotated goldfish genome, we had previously profiled the transcriptomic response of the goldfish brain under normoxic (21 kPa oxygen saturation, N) and hypoxic conditions (2.1 kPa oxygen saturation) after 1 and 4 weeks (1WH, 4WH). Using the RNA-Seq data, we report the occurrence of alternative mRNA splicing (skipped exon, retained intron, alternative 3' or 5' splice sites, and mutually exclusive exons). At 1WH/N, 1004 significant alternative splicing events on 769 gene loci were identified, increasing to 1187 on 963 loci at 4WH/N. There were 305 loci with alternatively spliced transcripts common to both 1WH/N and 4WH/N, 221 of which exhibited the same precise location and splicing mechanism. Specific gene transcripts affected by alternative splicing events were almost entirely different from previously identified differentially expressed genes under chronic hypoxia. GO-term enrichment analyses of gene loci of alternatively spliced transcripts, however, did include similar pathways as previously identified for DEGs. These include epigenetic machinery, ion channel activity (1WH/N), glutamate signaling (4WH/N), endothelial cell function, and ATP hydrolyzation pathways (1WH/N + 4WH/N). We describe selected examples of alternatively spliced transcripts to discuss possible functional relevance in the goldfish brain response to chronic hypoxia. Together, our data identified an additional layer of regulation in brain pathways relevant to hypoxia tolerance in goldfish, which complement previously reported gene expression changes.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"92"},"PeriodicalIF":2.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of estrogen receptor α and heat shock protein 90 in the apoptosis of fish liver cells after fipronil exposure. 雌激素受体α和热休克蛋白90在氟虫腈暴露后鱼肝细胞凋亡中的作用。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-05-07 DOI: 10.1007/s10695-025-01508-9

Rashid Alijani Ardeshir, Fatemeh Dehghani

{"title":"The role of estrogen receptor α and heat shock protein 90 in the apoptosis of fish liver cells after fipronil exposure.","authors":"Rashid Alijani Ardeshir, Fatemeh Dehghani","doi":"10.1007/s10695-025-01508-9","DOIUrl":"https://doi.org/10.1007/s10695-025-01508-9","url":null,"abstract":"Fipronil (FPN) as an insecticide can excessively enter aquatic ecosystems and may act as endocrine-disrupting chemicals by binding to estrogen receptor (ER) or aryl hydrocarbon receptor (AhR). Currently, there is limited information on the xenoestrogen role of FPN in the transcriptional modulation of hepatic genes involved in cell apoptosis. Three experiments were used in this study to determine how the FPN interference between the ER, AhR, and intermediate chaperons can induce apoptosis and change the expression of erα, erβ, erβ2, hsp70, hsp90, p53, bad1, bcl2, ahr, cyp1a, and caspase9 genes in common carp (Cyprinus carpio) hepatocytes. The IC50 values of FPN and 17β estradiol (E2) (positive control) in fish hepatocytes were determined (5 µg/mL). In the first experiment, exposure (6, 24, and 48 h) of hepatocytes to the low (0.1 µg/mL) and high (1 µg/mL) doses of FPN up-regulated apoptosis, pro-apoptotic genes (caspase9 and bad1), and chaperone protein genes (hsp70 and hsp90), while down-regulated anti-apoptotic genes (p53and bcl2). Additionally, there was a significant increase in the expression of the genes erα, ahr, and cyp1a that was dependent on both time and dose. ERα antagonist and a high dose of FPN were administered to the hepatocytes in the second experiment, which reduced cell apoptosis. In the third experiment, anti-apoptotic gene expression increased, and cell apoptosis and ahr and cyp1a gene expression significantly decreased when HSP90 and ERα antagonists were applied in comparison to the control group (P < 0.05). Based on this study, we demonstrate that FPN-induced apoptosis in fish hepatocytes is mediated by erα and hsp90 through transcriptional regulation of pro-apoptotic and anti-apoptotic genes.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"91"},"PeriodicalIF":2.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analysis of growth performance, antioxidant capacity, digestive enzyme activity, and transcriptomics provides insights into the chronic high-temperature stress response of brass gudgeon (Coreius heterodon) in the Yangtze River. 从生长性能、抗氧化能力、消化酶活性和转录组学等方面对长江黄铜鲟的慢性高温胁迫反应进行综合分析。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-05-06 DOI: 10.1007/s10695-025-01501-2

YaFan Dai, Leiming Zhang, Xingyu Ma, Xueying Pei, Bing Xie, Xiaolan Shi, Guanzheng Yang, Wei Liu, Guoqin Zhou

{"title":"Comprehensive analysis of growth performance, antioxidant capacity, digestive enzyme activity, and transcriptomics provides insights into the chronic high-temperature stress response of brass gudgeon (Coreius heterodon) in the Yangtze River.","authors":"YaFan Dai, Leiming Zhang, Xingyu Ma, Xueying Pei, Bing Xie, Xiaolan Shi, Guanzheng Yang, Wei Liu, Guoqin Zhou","doi":"10.1007/s10695-025-01501-2","DOIUrl":"https://doi.org/10.1007/s10695-025-01501-2","url":null,"abstract":"Global warming poses a serious threat to both wild and farmed fish. The brass gudgeon (Coreius heterodon) is a unique species of the Yangtze River and has important scientific value for studies of the impact of climate change. In this study, brass gudgeon were cultured for 40 days at 22 °C as control group, and at 27°C and 32°C as mild and severe high-temperature groups, respectively. After 40 days, the growth performance, liver antioxidant capacity, and intestinal digestive enzyme activities of each group were compared. Then, transcriptome sequencing analysis of liver and kidney of the 22°C and 32°C groups was performed. The results showed temperatures of 27°C and 32°C influenced growth performance, especially at 32 °C. High temperature caused varying degrees of damage to liver antioxidant capacity and intestinal digestive enzyme activities in brass gudgeon. Transcriptome analysis identified 1953 and 6709 differentially expressed genes (DEGs) in liver and kidney, respectively. We identified three candidate pathways through KEGG analysis, namely, \"protein process in the endoplasmic reticulum,\" \"toll-like receptor signaling pathway,\" and \"fatty acid degradation.\" DEGs involved in these pathways were mainly related to protein processing (HSP40, HSP70, and HSP90), immune system (TLR3, TLR8, IL1 β, and IL8), and lipid metabolism (FAD1, FADE, and ACAT), indicating that these functions might be adaptive regulatory mechanism of brass gudgeon in response to high-temperature stress. These results will help to elucidate the molecular mechanism of tolerance to high-temperature stress, enrich genetic resources, and provide reference data for large-scale artificial breeding of brass gudgeon.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"90"},"PeriodicalIF":2.5,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The first report of AMP-deaminase activity in skeletal muscles of Lates calcarifer and its stunning adaptation to ammonia poisoning. 钙化动物骨骼肌中amp -脱氨酶活性的首次报道及其对氨中毒的惊人适应。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-05-05 DOI: 10.1007/s10695-025-01504-z

Alireza Jahanbani, Ali Shahriari, Takavar Mohammadian

{"title":"The first report of AMP-deaminase activity in skeletal muscles of Lates calcarifer and its stunning adaptation to ammonia poisoning.","authors":"Alireza Jahanbani, Ali Shahriari, Takavar Mohammadian","doi":"10.1007/s10695-025-01504-z","DOIUrl":"https://doi.org/10.1007/s10695-025-01504-z","url":null,"abstract":"Ammonia poisoning is a common issue in fish breeding systems, leading to complications such as hypoxia and cellular energy crises. The AMP-deaminase enzyme plays a crucial role in maintaining the ATP/AMP ratio and responding to energy deficits. This study investigates the adaptation of AMP-deaminase in Asian sea bass (Lates calcarifer) to ammonia stress. A total of 150 fish were divided into two groups with densities of 6 g/L (control) and 14 g/L (densely stock fish), each replicated three times over 60 days. Ammonia levels increased significantly in both groups (P < 0.0001), with a higher concentration in the densely stock fish (> 1.6-fold increase compared to control, P < 0.001). The enzyme activity showed a significant enhancement in the densely stock fish, with Kcat increasing from 1.85 to 2.70 S-1 and Vmax decreasing from 11.99 to 8.10 μmol/mg s. The enzyme's stability was significantly higher in adverse conditions, as evidenced by an extended half-life (7 vs. 6 days in control, P < 0.05) and increased resistance to urea denaturation (I50 at 1.6 mM vs. 0.8 mM in control, P < 0.01). Optimal pH shifted from 7 (control) to 6 (densely stocked fish, P < 0.05), indicating an adaptation to acidic conditions. Additionally, enzyme activity remained stable under oxidative stress (H2O2 + FeSO4 exposure) and exhibited a significantly lower activation energy in the densely stock fish (14.1 vs. 17.98 kJ/mol, P < 0.05). These findings indicate that the AMP-deaminase enzyme in Lates calcarifer adapts to ammonia-induced hypoxia by modifying its kinetic properties and structural stability, enhancing muscle resilience under environmental stress. Further genetic and metabolic studies will strengthen these findings.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"89"},"PeriodicalIF":2.5,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin rhythms beyond the pineal organ: gene expression of receptors and biosynthesis enzymes in wild and F1 Senegalese sole. 褪黑激素节律超越松果体器官：受体和生物合成酶在野生和F1塞内加尔鳎的基因表达。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-05-01 DOI: 10.1007/s10695-025-01502-1

Francisca Félix, Catarina C V Oliveira, Ignacio Martín, Manuel Manchado, Francisco J Sánchez-Vázquez, Elsa Cabrita, Luisa M Vera

{"title":"Melatonin rhythms beyond the pineal organ: gene expression of receptors and biosynthesis enzymes in wild and F1 Senegalese sole.","authors":"Francisca Félix, Catarina C V Oliveira, Ignacio Martín, Manuel Manchado, Francisco J Sánchez-Vázquez, Elsa Cabrita, Luisa M Vera","doi":"10.1007/s10695-025-01502-1","DOIUrl":"https://doi.org/10.1007/s10695-025-01502-1","url":null,"abstract":"Fish gonadal melatonin production is still unexplored and could contribute to a better understanding of its role in reproduction control, especially for species with reproductive impairments. This study aimed to comprehend if Senegalese sole testes are an extra-pineal production site of melatonin and if it has seasonal and daily variations. Wild and F1 broodstocks were sampled in the breeding season (BS) and out of the reproductive season (OS), at mid-light (ML) and mid-dark (MD) daytimes. Blood plasma melatonin concentration was determined by radioimmunoassay (RIA). The expression of genes involved in melatonin biosynthesis (tph1a, tph2, hiomt1, aanat1a, aanat1b, and aanat2) and melatonin receptors (mel1, mel1c, and mel2) was evaluated in the brain, eye, and testis by quantitative real-time PCR (qPCR). Plasma melatonin concentration in wild sole displayed day/night differences in both seasons (average ML: 36 ± 22 pg/mL, MD: 108 ± 63 pg/mL), whereas differences in the F1 broodstock were only found OS (ML: 100 ± 54 pg/mL, MD: 187 ± 88 pg/mL). Gene expression of mel1 and mel2 receptors, and tph1a, aanat1a, aanat2, and hiomt1 enzymes was detected and quantified in the fish testes. Moreover, daily and seasonal fluctuations in the expression of those genes were found in all tissues and broodstock groups. However, the F1 group showed distinct gene expression patterns compared to the wild type, suggesting a disruption in the circadian system. This study revealed that Senegalese sole testes are a melatonin production site and, at the same time, suggested a dysregulation in the hypothalamus-pituitary-gonad (HPG) axis of F1 males.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"88"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hematological and blood biochemical responses of Colossoma macropomum and Piaractus brachypomus subjected to sudden temperature reduction and thermal readjustment. 突然降温和热调节对巨像和短腹巨像血液学和血液生化的影响。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-04-24 DOI: 10.1007/s10695-025-01499-7

Rafael José Furtado Souza, Sidney Dos Santos Silva, Narcia Carolina Santos da Silva, Imaculada de Morais Carvalho Ananias, Fábio Aremil Costa Dos Santos, André de Sena Sousa, Thamara Bentivole Magalhães, Camila Gomes de Oliveira, Suellen Cristina Moreira de Sales, Ronald Kennedy Luz

{"title":"Hematological and blood biochemical responses of Colossoma macropomum and Piaractus brachypomus subjected to sudden temperature reduction and thermal readjustment.","authors":"Rafael José Furtado Souza, Sidney Dos Santos Silva, Narcia Carolina Santos da Silva, Imaculada de Morais Carvalho Ananias, Fábio Aremil Costa Dos Santos, André de Sena Sousa, Thamara Bentivole Magalhães, Camila Gomes de Oliveira, Suellen Cristina Moreira de Sales, Ronald Kennedy Luz","doi":"10.1007/s10695-025-01499-7","DOIUrl":"https://doi.org/10.1007/s10695-025-01499-7","url":null,"abstract":"This study aimed to evaluate the physiological responses of Colossoma macropomum and Piaractus brachypomus, faced with a sudden reduction in temperature followed by thermal readjustment. Forty juveniles of each species were fasted for 24 h, after which, ten fish of each species were used as a basal group, while the others were subjected to a sudden temperature reduction from 28.7 to 18.6 °C and maintained in that condition for 24 h. The temperature was then restored, and the fish were observed for another 24 h of readjustment (R). Blood was collected at 1hATC, 24hATC and 24hR (ATC = after temperature change). There was no mortality during the experiment. C. macropomum showed a decrease in glucose without recovery after thermal readjustment, unlike P. brachypomus which experienced an increase at 1hATC. In both, C. macropomum and P. brachypomus, the lowest triglycerides levels were observed at 1hATC, with no return to the initial condition at 24hATC and 24hR. Cholesterol levels in both species showed reductions at 1hATC and 24hATC; however, there was a return to the initial condition at 24hR. The abrupt temperature change caused a reduction in lactate levels at 1hATC and 24hATC; however, only P. brachypomus returned to the initial levels. Plasma chloride was highest at 24hR and lowest at 1hATC, for both species. Therefore, we observed that the sudden drop in temperature, followed by thermal readjustment caused physiological changes, and P. brachypomus demonstrated a greater capacity to reestablish the initial condition of shape during thermal readjustment.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"87"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single and joint toxicity of ethoprophos and bispyribac-sodium to Oreochromis niloticus: biochemical and genotoxic responses. 乙磷磷和双嘧菌酯钠对尼罗褐蝽的单毒性和联合毒性：生化和基因毒性反应。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-04-23 DOI: 10.1007/s10695-025-01490-2

Kawther S El-Gendy, Eman M Mosallam, Aya S Abd El-Kader, Asmaa I Abdel Monem, Mohamed A Radwan

{"title":"Single and joint toxicity of ethoprophos and bispyribac-sodium to Oreochromis niloticus: biochemical and genotoxic responses.","authors":"Kawther S El-Gendy, Eman M Mosallam, Aya S Abd El-Kader, Asmaa I Abdel Monem, Mohamed A Radwan","doi":"10.1007/s10695-025-01490-2","DOIUrl":"https://doi.org/10.1007/s10695-025-01490-2","url":null,"abstract":"Pesticides can bioaccumulate in fish tissues, and there are serious concerns around the world about their effects on consumer health. The objective of this study was to highlight the effects of two commercial pesticides, ethoprophos and bispyribac-sodium, commonly used on high-consumption crops in Egypt, either individually or in mixture, on some biomarkers of Nile tilapia, Oreochromis niloticus, an important commercial fish species. Low concentrations of ethoprophos (96 µg/L) and bispyribac-sodium (1.28 µg/L) were selected to evaluate the effect of these substances on neurotoxic marker (acetylcholine esterase, AChE), liver function parameters (alkaline phosphatase, ALP; alanine aminotransferase, ALT; and aspartate aminotransferase, AST), and renal function parameters (creatinine and urea), as well as genotoxic marker (micronuclei, MN; and other nuclear abnormalities) during variable periods (7, 14, 21, and 28 days). The results demonstrate that ethoprophos and bispyribac-sodium pose a risk to native freshwater fish by causing detrimental effects. Both compounds, separately and in combination, induced neurotoxicity, hepatorenal biomarkers inductions, and increases in MN frequency and other erythrocytic nuclear abnormalities in a time-dependent manner. Moreover, the mixture displayed both synergistic and antagonistic interactions for examined parameters. This study highlights the importance of using validated biomarkers to monitor fish health, which may be utilized as early alarms of environmental risks.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"86"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12018621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of graded inclusion of black soldier fly (Hermetia illucens, Linnaeus, 1758) pre-pupae meal in diets for gilthead seabream (Sparus aurata, Linnaeus, 1758) on gut microbiome and liver morphology. 分级加入黑兵蝇（Hermetia illucens, Linnaeus, 1758）蛹前饲料对金头海鲷（Sparus aurata, Linnaeus, 1758）肠道微生物组和肝脏形态的影响。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-04-22 DOI: 10.1007/s10695-025-01485-z

Marco Basili, Basilio Randazzo, Letteria Caccamo, Stefano Guicciardi O Guizzardi, Martina Meola, Anna Perdichizzi, Grazia Marina Quero, Giulia Maricchiolo

{"title":"Effect of graded inclusion of black soldier fly (Hermetia illucens, Linnaeus, 1758) pre-pupae meal in diets for gilthead seabream (Sparus aurata, Linnaeus, 1758) on gut microbiome and liver morphology.","authors":"Marco Basili, Basilio Randazzo, Letteria Caccamo, Stefano Guicciardi O Guizzardi, Martina Meola, Anna Perdichizzi, Grazia Marina Quero, Giulia Maricchiolo","doi":"10.1007/s10695-025-01485-z","DOIUrl":"https://doi.org/10.1007/s10695-025-01485-z","url":null,"abstract":"Over the last decades, an insect meal has received great attention for finfish diets, due to its nutritional composition and low ecological footprint. In the present study, we assessed the response of gut microbiota composition and liver histology of gilthead seabream (Sparus aurata) fed four experimental diets including the black soldier fly (Hermetia illucens) meal (HI) used to replace 0 (HI0), 25 (HI25), 35 (HI35) and 50 (HI50) percent of fish meal in a 131-day feeding trial. At the end of the experiment, a remarkable change in gut microbiota composition related to HI inclusion was observed, with a preponderance of Cyanobacteriain the control and low HI groups (HI0, HI25) while Chloroflexi became prevalent in the higher HI inclusion groups (HI35, HI50). Predictive analysis on bacterial metabolic pathways showed a clear separation between HI0-HI25 and HI35-HI50 groups. The microbiota shifts observed suggest a pivotal role of HI in inducing a bacterial-mediated physiological response in this fish species, probably due to chitin content and the fatty acid profile of this ingredient. Liver histology showed a higher hepatocyte size in fish from the HI50 group, suggesting lipid dysmetabolism due to the HI meal fatty acid profile, while a marginal adaptive response was observed in the HI25 group. In conclusion, while up to 25% inclusion of black soldier fly meal showed limited adverse effects, 50% HI dietary inclusion is not recommended in gilthead seabream diet, since possible alteration in lipid deposition, particularly at hepatic level, were highlighted in this fish species.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"85"},"PeriodicalIF":2.5,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12014712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aluminum chloride (AlCl₃) alters the physiological response of rainbow trout. 氯化铝（AlCl3）改变虹鳟鱼的生理反应。

IF 2.5 3区农林科学

Fish Physiology and Biochemistry Pub Date : 2025-04-22 DOI: 10.1007/s10695-025-01497-9

Daniela Nualart, Daniela Diaz, Jaime Tapia, Carolina Quinteros, Luis Vargas-Chacoff

{"title":"Aluminum chloride (AlCl3) alters the physiological response of rainbow trout.","authors":"Daniela Nualart, Daniela Diaz, Jaime Tapia, Carolina Quinteros, Luis Vargas-Chacoff","doi":"10.1007/s10695-025-01497-9","DOIUrl":"https://doi.org/10.1007/s10695-025-01497-9","url":null,"abstract":"Fish production in terrestrial recirculating aquaculture systems (RAS) has steadily increased over the past decades. Therefore, several studies, both in freshwater and seawater, have shown that increased water reuse in RAS leads to increased levels of heavy metals in the bodies of fish. The objective of the present study was to evaluate the impact on the physiology, osmoregulatory mechanisms, and antioxidant response in fingerlings of rainbow trout (Oncorhynchus mykiss) exposed to varying doses of AlCl3 within a recirculation system. Changes in gills, N+-K+-ATPase activity and H+-ATPase activity, plasma osmolality, lactate, glucose, chloride levels, and gills and liver oxidative stress responses were used to monitor physiology responses. As a result, AlCl3 exposure modified responses in rainbow trout. Plasma parameters increased during the 6-day exposure, while lactate levels decreased in both doses. The enzymatic activities of antioxidant enzymes and the NKA pump increased when fish were exposed to high and low doses of aluminum chloride. Gene expression of glutathione reductase and peroxidase transcripts increased and decreased respectively in the liver, contrary to that observed in gill tissue. AlCl3 was also observed in liver and gill tissues. This study shows that exposure to AlCl3 affects ion regulation and oxidative stress in rainbow trout fry.","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"84"},"PeriodicalIF":2.5,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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