Fish Physiology and Biochemistry最新文献

{"title":"Retrospect of fishmeal substitution in largemouth bass (Micropterus salmoides): a review.","authors":"Yuanyi Liu, Changchang Pu, Zhuo Pei, Weichuan Zhang, Zihui Wei, Hongyu Chen, Yong Huang","doi":"10.1007/s10695-024-01429-z","DOIUrl":"10.1007/s10695-024-01429-z","url":null,"abstract":"<p><p>With the growth of the population, the demand for aquatic products is increasing. Additionally, the development of the aquaculture industry has led to a heightened demand for fishmeal (FM). FM is a high-protein feed raw material made from one or more types of fish, which has been deoiled, dehydrated, and crushed. The world's major FM-producing countries include Peru, Chile, Japan, Denmark, etc., among which exports from Peru and Chile account for about 70% of the total trade volume. However, in recent years, global warming, environmental pollution, and overfishing have gradually declined marine fishery resources. The shortage of high-quality FM and its rising prices have become a significant constraint to the development of fisheries. Consequently, aquaculture nutritionists are actively seeking solutions to reduce the reliance on FM by either enhancing the utilization rate of existing FM or developing new protein sources as substitutes. The challenge of FM replacement has thus emerged as a significant global issue. Largemouth bass (LMB) is one of the more cultured freshwater fishes in the world and is popular among consumers for its delicious and delicate flesh and rich and diverse nutrition. The protein content in feed is an essential factor affecting LMB growth and feed cost. LMB protein requirement is about 40-50%, and the amount of FM added accounts for about 50% of the protein feed. This article reviews the current research status of alternative protein sources, including plant proteins, livestock and poultry by-product proteins, insect proteins, and single-cell proteins. This research is significant for exploring feed formulation and cost reduction for LMB.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"21"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplementation of sea buckthorn (Hippophae rhamnoides L.) oil in the diets of freshwater rainbow trout (Oncorhynchus mykiss W.) led to enhanced growth and better meat quality.","authors":"Hilal Bayir, Telat Yanik","doi":"10.1007/s10695-024-01433-3","DOIUrl":"10.1007/s10695-024-01433-3","url":null,"abstract":"<p><p>In this study, sea buckthorn oil (SBO) was added to rainbow trout fish diets at 0.25%, 0.5%, and 1% rates in order to determine the effects on growth, feed conversion rate, survival rate, fillet quality, and physiological, biochemical and histopathological parameters. The experiment was designed in four groups with three replicates each including 20 fish with 18 ± 3 g and lasted for 45 days. Fish were fed three times a day and weighed biweekly. At the end of the experiment, significant differences were determined with respect to specific growth rates (SGR), weight gains (WG), and feed conversion ratios (FCR) (P < 0.05), except for survival ratios (SR) (P > 0.05). Sea buckthorn oil improved fish fillet quality and weight gain rates by up to 27% compared to control. The pepsin, amylase, lipase, and trypsin enzyme activities differed significantly (P < 0.05) from those of the control group. Serum lysozyme myeloperoxidase (MPO) and plasma nitro blue tetrazolium (NBT) levels differed significantly (P < 0.05) in the 0.5% and 1% groups compared to the control group, except for the 0.25% group. Liver catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA) levels significantly differed in all groups, especially in the 0.5% and 1% groups compared to the control group (P < 0.05). SBO supplementation to feed caused significant changes in the FA profiles, especially 18:00 in ∑SFA; 16:1 in ∑MUFA; 20:5n-3 and 20:2n-6 in ΣPUFA; 20:5n-3 in ∑n-3; and 20:2n-6 in ∑n-6 in rainbow trout (P < 0.05). There were no histopathological changes between the control and the experimental groups. The determined data suggested that 1% SBO may be successfully used as a feed additive in rainbow trout.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"18"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute low temperature and lipopolysaccharide differentially modulated the innate immune and antioxidant responses in a subtropical fish, the pacu (Piaractus mesopotamicus).","authors":"Mariana Maluli Marinho de Mello, Allan Emilio Piedade, Camila de Fátima Pereira de Faria, Elisabeth Criscuolo Urbinati","doi":"10.1007/s10695-024-01425-3","DOIUrl":"10.1007/s10695-024-01425-3","url":null,"abstract":"<p><p>Exogenous factors such as low water temperature can be stressful and elicit negative immune system effects, especially for fish, which are ectothermic. Stress and immune responses require energy overload, which can affect the cellular redox balance, causing oxidative damage. These overall responses impair the animal's health and negatively affect fish farming. To evaluate indicators of stress, immune and antioxidant systems, and oxidative stress responses in fish during thermal challenge, the present study reduced the water temperature from 29.5 °C to 16 °C and then inoculated pacu (Piaractus mesopotamicus) with lipopolysaccharide (LPS) from Escherichia coli. Our results revealed that acute exposure to low water temperature itself increased blood glucose, impaired the serum lysozyme concentration and increased GSH-Px activity. There was an interaction effect between low temperature and LPS inoculation. After LPS inoculation, leukocytes were initially activated (3 h); glucose levels increased (3 h); GST activity initially decreased (3 h) but then increased (6 h); SOD, CAT and GSH-Px activities decreased; and lysozyme activity remained depressed in fish subjected to cold shock. The results showed that thermal and immunological challenges impaired the maintenance of leucocyte activation and compromised the pacu oxidant response. The overall response of pacu to thermal challenge indicates that the species proved to be acutely sensitive to a drop in water temperature, reducing its ability to maintain homeostasis, especially when subjected to immunological challenge.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"12"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tryptophan-supplemented diet modulates the metabolic response of European seabass (Dicentrarchus labrax) juveniles reared under space-confined conditions and submitted to acute inflammation.","authors":"Diogo Peixoto, Juan Antonio Martos-Sitcha, Benjamín Costas, Rita Azeredo, Juan Miguel Mancera","doi":"10.1007/s10695-024-01427-1","DOIUrl":"10.1007/s10695-024-01427-1","url":null,"abstract":"<p><p>The energetic costs of being in stressful conditions require the involvement of hormones associated with metabolic support, which may also influence immune function. The present work aimed to explore the links between tryptophan nutrition and metabolic responses in European seabass (Dicentrarchus labrax) held under space-confined conditions, and subsequently submitted to an immune challenge. To study that, two dietary treatments were evaluated, i.e. control diet (CTRL) and CTRL-based diet supplemented with tryptophan (0.3%; TRP) to fish under space-confinement conditions (10 kg/m³) or not (5 kg/m³). Dietary treatments were offered for 15 days after which fish were intraperitoneally injected (i.p.) with Photobacterium damselae piscicida. Liver was sampled before the immune challenge (at end of the nutritional trial, 0 h) and at 4, 24, 48 and 72 h post-injection. Fish-fed TRP exhibited distinct metabolic profiles compared to those fed CTRL diets, particularly in energy metabolism and stress response. CTRL-fed fish in space-confined conditions showed a gradual reduction of lipid oxidative enzyme activity post-injection, pointing to acute stress-induced lipid catabolism feedback. In contrast, stressed fish fed TRP under same rearing conditions presented reduced glucose levels and cortisol production but unchanged 3-hydroxiacil-CoA dehydrogenase (EC 1.1.1.35, HOAD) activity patterns, suggesting an inhibitory and modulatory role of tryptophan in stress response. Discriminant analysis revealed that fish fed TRP under space-confined conditions resembled fish CTRL-fed under non-stressful conditions. These findings suggest that tryptophan dietary supplementation for stressed fish modulates their metabolic responses and potentially mitigates the negative effects of rearing stressful conditions after subsequent acute stress induced by an immune challenge.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"10"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11634932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification of glutathione S-transferase enzyme from liver tissue of shabout (Barbus grypus Heckel) and investigation of the inhibition effect of some metal ions under in vitro conditions.","authors":"Bekir Çiftçi, Arzu Koçak Mutlu, Ebru Akkemik","doi":"10.1007/s10695-024-01424-4","DOIUrl":"10.1007/s10695-024-01424-4","url":null,"abstract":"<p><p>Shabout is a fish with high nutritional value and economic potential. This fish is exposed to environmental factors due to the metal toxicity in its habitat and, consequently, its diet. The main purpose of this study was to determine how the detoxification mechanism of shabout is affected by examining the interaction of glutathione s-transferase enzyme with heavy metals. In our study, elemental analysis was first performed with the ICP-OES on water samples taken from three different points to detect metal toxicity in the habitat of the shabout. Then, the GST enzyme from the liver tissue of the shabout was purified for the first time by our team using the glutathione agarose affinity chromatography technique. Finally, the inhibition effects of nitrate salts of some metal ions on the purified enzyme activity were investigated under in vitro conditions. It was determined that the water courses where the fish were sampled in our study were alarming in terms of heavy metal content. While shabout liver showed Fe³⁺ activation effect on GST enzyme under in vitro conditions, metal ions Na⁺, Zn²⁺, Co²⁺, Cu²⁺ and Pb²⁺ showed inhibition effects. The detected inhibition concentration range showed that the enzyme is quite resistant to the metal salts whose effects were examined. It was concluded that in the consumption of shabout, emphasized to be a nutritious fish, the heavy metal levels of the region where the fish is caught should be taken into account and catching should be made in water resources inspected by relevant experts.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"28"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of acclimation to chronic intermittent hypoxia in the gills of largemouth bass (Micropterus salmoides).","authors":"Qiao Liu, Hong Wang, Jiayu Ge, Lipeng Guo, Rabia Tahir, Jie Luo, Kuo He, Haoxiao Yan, Xin Zhang, Quanquan Cao, Zhang Cheng, Liulan Zhao, Song Yang","doi":"10.1007/s10695-024-01419-1","DOIUrl":"10.1007/s10695-024-01419-1","url":null,"abstract":"<p><p>The acclimation response of fish gills to chronic intermittent hypoxia (CIH) is an important aspect to understand, as anthropogenically induced hypoxia in water bodies has been a stressor for fish for many years and is expected to persist in the future. In order to investigate the acclimation response of fish gills to CIH stress, we conducted a study using largemouth bass (Micropterus salmoides) exposed to intermittent hypoxia (dissolved oxygen level, 2.0 mg·L^-1) for either 1 or 3 h per day, over a period of 8 weeks. Our findings indicate that exposure to CIH induced remodeling of the gills and an increase in gill surface area. This remodeling of the gills may be attributed to changes in cell growth and proliferation, which are influenced by the activation of the MAPK signaling pathway. We also observed significant upregulation of genes related to glycolysis (fba, pgam1, pepck, atp-pfk, pfk-2, g6pi, gapd-1, and pk), while genes associated with cholesterol synthesis (3β-hsd, cyp51, dsdr- × 1, dsdr, and dhcr7) were downregulated following CIH exposure. Furthermore, we observed the presence of elongated megamitochondria in mitochondria-rich cells within the gills of fish exposed to hypoxia. Additionally, numerous genes involved in calcium signaling pathways were upregulated in the gills of largemouth bass, suggesting an enhanced sensitivity of gills to environmental cues in hypoxia conditions. However, the expression levels of certain genes related to innate and adaptive immune responses were inhibited following CIH exposure. Moreover, the number of mucous cells decreased after CIH exposure. This may have made the gills more susceptible to infection by pathogens, although it facilitated oxygen uptake. These findings highlight the potential vulnerability of gills to pathogenic organisms in the presence of CIH. Overall, our study contributes to a better understanding of how fish acclimate to CIH.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"22"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of carp sperm respiration: fluorometry with optochemical oxygen sensor versus polarography.","authors":"Iryna Musatova, Borys Dzyuba, Serhii Boryshpolets, Azeem Iqbal, Anatolii Sotnikov, Vitaliy Kholodnyy, Viktoriya Dzyuba","doi":"10.1007/s10695-024-01418-2","DOIUrl":"10.1007/s10695-024-01418-2","url":null,"abstract":"<p><p>The primary function of spermatozoa is to fertilize the oocyte, which depends on their motility and is directly associated with their metabolic state. The oxygen consumption rate (OCR) of spermatozoa reflects the respiratory capacity of sperm mitochondria under various physiological conditions and is an essential marker of sperm quality. We determined the OCR of common carp (Cyprinus carpio) sperm using two respirometry methods: the conventionally used polarographic method with a Clark-type electrode and fluorometric assay with an Oxo Dish optochemical oxygen sensor. The latter was used for the first time to evaluate spermatozoa oxygen consumption in various metabolic states (under different treatments) at different dilution rates. These two methods were compared using Bland-Altman analysis, and the applicability of the optochemical oxygen sensor for evaluating carp sperm oxygen consumption was discussed. Sperm motility and progressive velocity parameters were also assessed to evaluate the effect of sperm respiration under different metabolic states and dilution rates and preincubation period on the physiological status of spermatozoa. The comparison of these respirometry methods clearly shows that while the polarographic method allows immediate measurement of oxygen levels after adding a sperm sample, the optochemical oxygen sensor has a priority in the amount of data obtained due to simultaneous measurements of several samples (e.g., different males, different fish species, repetitions of the same sample or various experimental conditions), even at a later time after adding sperm to the measuring chamber. However, the compared methods are complementary, and the proposed methodology can be applied to other fish species.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"16"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurotrophins and their receptors in the peripheral nervous system and non-nervous tissue of fish.","authors":"Aldo Isaac Carrillo-Muñoz, Sharet Y R-Jaimes, Guadalupe C Hernández-Hernández, Francisco Castelán","doi":"10.1007/s10695-025-01453-7","DOIUrl":"10.1007/s10695-025-01453-7","url":null,"abstract":"<p><p>Trophic factors, such as neurotrophins, are fundamental for cellular processes including differentiation, growth, survival, and regeneration. These molecules exhibit significant morphological and phylogenetic conservation throughout the animal kingdom, indicating conserved functions. In fish, the oldest and most diverse group of vertebrates, neurotrophins, and their receptors play pivotal roles not only within the central nervous system but also in various peripheral tissues. They are distributed in mechanosensory, muscle, skin, respiratory, circulatory, digestive, endocrine, urinary, reproductive, and immune systems, suggesting their involvement in the development and maintenance of all tissues/organs/systems. Despite this broad distribution, studies focusing on these molecules outside of the central nervous system have been limited to just 12 fish species. These investigations have revealed diverse expression patterns across different ages and tissues/organs/systems, expanding our comprehension of their functions beyond the central and peripheral nervous systems. Notably, BDNF and NT-3 are prominently expressed outside the central nervous system, particularly in mechanosensory and digestive tissues, whereas NGF is predominantly observed in mechanosensory and urinary systems. The expression and localization of neurotrophins and their receptors vary among organs, underscoring tissue-specific roles. Further research is imperative to decipher the precise functions and mechanisms of action of neurotrophins and their receptors in diverse fish tissues. Enhanced efforts are needed to include a broader range of fish species in these studies to advance our understanding of these agents in complex vertebrates, thereby shedding light on tissue development, regeneration, and maintenance, with potential implications for addressing organ-related issues.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"38"},"PeriodicalIF":2.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Tryptophan‑supplemented diet modulates the metabolic response of European seabass (Dicentrarchus labrax) juveniles reared under space‑confined conditions and submitted to acute inflammation.","authors":"Diogo Peixoto, Juan Antonio Martos-Sitcha, Benjamín Costas, Rita Azeredo, Juan Miguel Mancera","doi":"10.1007/s10695-025-01451-9","DOIUrl":"10.1007/s10695-025-01451-9","url":null,"abstract":"","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"37"},"PeriodicalIF":2.5,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11779781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cardiac response of the goldfish Carassius auratus to environmental hypoxia: from hemodynamics to mitochondria.","authors":"Mariacristina Filice, Rosa Mazza, Alfonsina Gattuso, Alessia Caferro, Gaetana Napolitano, Gianluca Fasciolo, Paola Venditti, Sandra Imbrogno, Maria Carmela Cerra","doi":"10.1007/s10695-025-01452-8","DOIUrl":"10.1007/s10695-025-01452-8","url":null,"abstract":"<p><p>Under low O₂, the heart of Carassius auratus (goldfish) shows an enhanced hemodynamics. This is observed in ex vivo cardiac preparations from animals acclimated to both normoxia and short-term (4 days) moderate hypoxia and perfused for 90 min with a hypoxic medium. Under short-term hypoxia, this is associated with a higher ventricular muscularity and an expanded mitochondrial compartment. To date, little is known about the putative influence of hypoxia on the mitochondrial contribution to cardiac energy metabolism. Similarly, it remains unexplored whether the exposure to environmental low O₂ affects the cardiac response to preload increases (i.e., the Frank-Starling mechanism). We here observed, on ex vivo isolated and perfused goldfish heart, that 20 days of exposure to moderate water hypoxia are accompanied by a potentiated cardiac performance, analyzed as stroke volume, cardiac output, and stroke work. The sensitivity to preload increases significantly improved after 20 days of hypoxia, while it is similar to normoxia after 4 days of exposure. This suggested a time-dependent response. Mitochondrial O₂ consumption initially decreased during short-term hypoxia but returned to normoxia-like levels after 20 days of exposure. Biomolecular analyses of ventricular extracts revealed a time-dependent regulation of key proteins involved in the mitochondrial biogenesis, including PGC1α, NRF1/2, and TFAM, as well as cytochrome c. Additionally, mitochondrial DNA content was notably increased after 20 days of hypoxia. Our data revealed that, when challenged by chronic environmental hypoxia, the goldfish heart improves its pumping behavior under both basal and loading-stimulated conditions. This is accompanied by a mitochondrial remodeling which likely supports adequate energy supply for the working myocardium.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"36"},"PeriodicalIF":2.5,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}