Fish Physiology and Biochemistry最新文献

{"title":"Morpho-physiological digestive features of genetically closely related sympatric lacustrine whitefish forms with different feeding habits.","authors":"Mikhail Solovyev, Anastasiya Shokurova, Ekaterina Kondakova, Vadim Vasilenko, Nikolai Bochkarev, Elena Kashinskaya, Enric Gisbert","doi":"10.1007/s10695-024-01440-4","DOIUrl":"https://doi.org/10.1007/s10695-024-01440-4","url":null,"abstract":"<p><p>The effect of different feeding habits on gut morphology and digestive function has been intensively studied during the last decades but sympatric closely related fishes are relatively rare objects of such studies. In the present study, we have identified both morphological and physiological changes in the digestive system of a sympatric pair of whitefish represented by \"normal\" Coregonus lavaretus pidschian (benthivorous) and \"dwarf\" C. l. pravdinellus (planktivorous) forms. There were statistically significant differences in terms of histometric variables between segments of the stomach (height of lining epithelium, fold thickness and height values, and number of folds) and intestine (enterocyte height and fold height) within each form as well as between forms for the same segment of the digestive tract like in the stomach (height of lining epithelium and fold thickness) and intestine (enterocyte height, fold height and fold thickness). Both forms had a similar number (8-9 bands) and molecular weight of isoforms/isoenzymes of alkaline proteases inhibited by PMSF (serine proteases) based on zymogram analyses. The maximum level of activity of alkaline proteases, alkaline phosphatase, and aminopeptidase N were registered in the pyloric caeca for both forms. In contrast to these enzymes, the activity of α-amylase as well as of non-specific lipases and esterases in the pyloric caeca was similar or even lower if compared to other intestinal segments. In conclusion, both sympatric forms of whitefish showed some similarities in several of studied biochemical and histological parameters of their digestive tracts, regardless of their different feeding habits, which might be related to their genetical background.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"34"},"PeriodicalIF":2.5,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Muscle biochemistry and residue accretion in male Oreochromis niloticus fries administered therapeutic, subtherapeutic and overdoses of dietary oxytetracycline.","authors":"Thangapalam Jawahar Abraham, Anwesha Roy, Jasmine Singha, Ravindran Rajisha, Ranjit Kumar Nadella, Prasanna Kumar Patil","doi":"10.1007/s10695-024-01447-x","DOIUrl":"https://doi.org/10.1007/s10695-024-01447-x","url":null,"abstract":"<p><p>Oxytetracycline (OTC), an approved antibiotic for aquaculture, is under strict control and regulatory endeavour. This study compared the effects of oral administration of graded doses of OTC comprising the therapeutic (80 mg/kg biomass/day), subtherapeutic (40 mg) and overdoses (240, 400 and 800 mg) in male Nile tilapia Oreochromis niloticus fries (0.64 ± 0.02 g) when fed for 10 consecutive days and observed for 22 days post-OTC-dosing (POD) period. A dose-dependent reduction in food intake, survival and muscle calcium, chloride, malondialdehyde and superoxide dismutase was observed, while the muscle glucose significantly increased. However, the changes were reversible with dose cessation. The fries of the therapeutic dose group recorded the maximum biomass, while the overdosed groups recorded a significant decline in weight gain. On day 10 of dosing, all groups' muscle OTC residues peaked. The residues in the subtherapeutic and therapeutic dose groups were lowered below the maximum residue limit (MRL) of 100 ng/g on day 10 POD. The residues were further reduced close to the MRL in the overdosed groups on day 22 POD. The fries tolerated the therapeutic dose well and showed effective adaptive responses. Considering muscle residue accretion and physiological responses, OTC can be endorsed as a safe drug for in-feed administration to tilapia fries. However, the sensible use of this approved drug is essential for sustainable aquaculture.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"35"},"PeriodicalIF":2.5,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological, biochemical, apoptosis-linked gene expression, and histopathological insights in Nile catfish subjected to toxicity by magnetite nanogel.","authors":"Yasmina K Mahmoud, Shaimaa A A Ahmed, Heba H Mahboub, Abdelwahab A Abdelwarith, Elsayed M Younis, Ahmed A Elnegiry, Sherif M Shawky, Sahar H Orabi, Sameh H Ismail, Simon J Davies, Mahmoud I M Darwish","doi":"10.1007/s10695-024-01420-8","DOIUrl":"https://doi.org/10.1007/s10695-024-01420-8","url":null,"abstract":"<p><p>The target of this novel work is to assess the immunosuppression, genotoxicity, histopathological alterations, and cumulative mortality induced by acute toxicity of magnetite nanogel (MNG) in Nile catfish. Furthermore, a subsequent 10-day depuration period is adopted to estimate the restoration of those disturbed indices. Nile catfish (n = 180) were allotted into four groups and exposed to different concentrations of MNG (0, 1/10, 1/8, and 1/5 96-h LC₅₀). The outcomes displayed a remarkable concentration-dependent decline in the immune indices level (LYZ, NO, and MPO). The gene expression of antioxidant (SOD) and apoptosis-regulatory genes (MAPK3 and JNK) was upregulated in MNG-exposed fish. Histopathological disruptions in the tissue architecture of gills and intestines were also evident in MNG-exposed groups. Interestingly, after a 10-day depuration period, marked improvement in the level of immune response mediators and gene expression of apoptotic genes was evident in all experimental groups, particularly at 1/10 of LC₅₀. Overall, exposure to higher concentrations of MNG (1/5 and 1/8 of LC₅₀) exerts multiple adverse influences on the Nile catfish, which were less pronounced at 1/10 of LC₅₀. Surprisingly, a subsequent 10-day recuperation time was adequate for alleviating those impairments, with the maximum improvement in the Nile catfish exposed to 1/10 of LC₅₀.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"31"},"PeriodicalIF":2.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143055901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ontogeny of adenohypophyseal cells, pituitary gland development, and structure in adults of Astyanax lacustris (Teleostei, Characiformes): an emerging Neotropical model fish species.","authors":"Giovana Souza Branco, Monica Cassel, Chayrra Chehade, Marília de Paiva Camargo, Gisele Cristiane de Melo Dias, Maria Ines Borella, Lázaro Wender Oliveira de Jesus","doi":"10.1007/s10695-024-01448-w","DOIUrl":"https://doi.org/10.1007/s10695-024-01448-w","url":null,"abstract":"<p><p>Pituitary gland morphogenesis and the ontogeny of the adenohypophyseal (AH) cells of Astyanax lacustris are presented herein. This Characiformes species shows great ecological and commercial importance, and it has been increasingly used as animal model. For this study, A. lacustris specimens were collected from 0.5 to 120 days after hatching (dah) (adults). The entire animal or its head was appropriately fixed, and after histological processing, the sections were subjected to histochemical and immunohistochemical reactions, using homologous and heterologous antibodies. The first AH cells of A. lacustris were detected at 1 dah by the immunostaining of prolactin (PRL)-producing cells. The morphology of the gland presented changes in shape throughout the development, starting with elongation but more oval at the end. The neurohypophysis was differentiated at 3 dah, along with the identification of adrenocorticotropic hormone (ACTH), melanotropic hormone (MSH), thyroid-stimulating hormone (TSH), and follicle-stimulating hormone (FSH)-producing cells. Identification of the immunoreactive cells to anti-luteinizing hormone (LH), anti-somatolactin (SL), and anti-growth hormone (GH) antibodies occurred at 5 dah. At 20 dah, an increase in pituitary proportions and the presence of the pituitary stalk were observed. At 60 dah, the pituitary gland already had the same shape and distribution of AH cells seen in the adult. The ontogeny of adenohypophyseal cells in A. lacustris corroborates the heterogeneity in the appearance of these cell types in teleosts and suggests that these hormones actively participate during the post-hatching development of this species, even before the establishment of all endocrine axes. Our findings contribute to understanding the morphogenesis of the hypothalamic-pituitary axis in South American teleosts, providing essential data for the development of future studies related to pituitary gland morphophysiology under normal or experimental conditions.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"33"},"PeriodicalIF":2.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary deacetylated chitin nanoparticles confer protection against diazinon toxicity in male African catfish: evaluation of immune-biochemical, antioxidant, and reproductive profiles.","authors":"Hiam Elabd, Heba H Mahboub, Heba S Hamed, Abdelwahab A Abdelwarith, Elsayed M Younis, Samar Kamel, Amany Ramah, Sahar H Orabi, Sherif M Shawky, Simon J Davies, Zeinab Hassan","doi":"10.1007/s10695-024-01414-6","DOIUrl":"https://doi.org/10.1007/s10695-024-01414-6","url":null,"abstract":"<p><p>Currently, deacetylated chitin (chitosan) nanoparticles (CNPs) are successfully utilized in aquaculture practices. This trial demonstrates the efficacy of CNPs in combating diazinon (DZN) toxicity in African catfish, Clarias gariepinus, via monitoring hepato-renal function, serum immune trait, hormonal function, and hepato-renal antioxidant activity. Four groups were allocated as follows: a control group, a CNPs group (0.66 ml/L CNPs), a DZN exposed group (0.598 ppm, 1/10 LC50), and a DZN + CNPs group (0.598 ppm DZN + 0.66 ml/L CNPs), all for 30 days. Exposure to 0.598 PPm DZN resulted in a severe decline in the immune parameters (albumin, globulin, immunoglobulins (IgG, IgM), and total proteins), neurological indicator, acetylcholinesterase (AchE), reproductive hormones (Testosterone (T.) and Luteinizing Hormone (LH)), and the superoxide dismutase (SOD) and total antioxidant capacity (TAC) readings in both hepatic and renal samples. Moreover, a clear increment in hepatic and renal indicators (AST, ALT, urea, and creatinine), lipid peroxidation (LPO), and some reproductive indices including follicle stimulating hormone (FSH) and serum 17-β estradiol (E2) was clearly increased. Interestingly, the dietary inclusion of CNPs markedly palliated the toxicity by DZN with significant improvement in the immune-reproductive indices, plus normalizing the values of hepato-renal function and augmenting the activity of antioxidant parameters. Thus, the present study demonstrates the efficacy of CNPs in mitigating low-dose DZN toxicity, resulting in significant improvements in physiological, biochemical, and reproductive parameters. This highlights the promising potential of CNPs as a viable strategy for enhancing the health of C. gariepinus, thereby promoting the sustainability of the aquaculture industry and safeguarding human health.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"32"},"PeriodicalIF":2.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential effects of chronic unpredictable stress on behavioral and molecular (cortisol and microglia-related neurotranscriptomic) responses in adult leopard (leo) zebrafish.","authors":"Tatiana O Kolesnikova, Nikita O Prokhorenko, Sahil V Amikishiev, Vadim S Nikitin, Anton D Shevlyakov, Aleksey N Ikrin, Radmir R Mukhamadeev, Anastasiia D Buglinina, Kirill V Apukhtin, Anastasia M Moskalenko, Nikita P Ilyin, Murilo S de Abreu, Konstantin A Demin, Allan V Kalueff","doi":"10.1007/s10695-024-01446-y","DOIUrl":"https://doi.org/10.1007/s10695-024-01446-y","url":null,"abstract":"<p><p>Stress plays a key role in mental, neurological, endocrine, and immune disorders. The zebrafish (Danio rerio) is rapidly gaining popularity as s model organism in stress physiology and neuroscience research. Although the leopard (leo) fish are a common outbred zebrafish strain, their behavioral phenotypes and stress responses remain poorly characterized. Here, we examined the effects of a 5-week chronic unpredictable stress (CUS) exposure on adult leo zebrafish behavior, cortisol levels, and brain gene expression. Compared to their unstressed control leo counterparts, CUS-exposed fish showed paradoxically lower anxiety-like, but higher whole-body cortisol levels and altered expression of multiple pro- and anti-inflammatory brain genes. Taken together, these findings suggest that behavioral and physiological (endocrine and genomic) responses to CUS do differ across zebrafish strains. These findings add further complexity to systemic effects of chronic stress in vivo and also underscore the importance of considering the genetic background of zebrafish in stress research.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"30"},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological responses of pacu (Piaractus mesopotamicus) to intermittent cold exposure: A comprehensive analysis of stress, immunity, antioxidant, and metabolic adaptations.","authors":"Camila de Fátima Pereira de Faria, Beatriz Ullian de Andrade, Elisabeth Criscuolo Urbinati","doi":"10.1007/s10695-024-01445-z","DOIUrl":"10.1007/s10695-024-01445-z","url":null,"abstract":"<p><p>This study examined the energy-dependent physiological responses, including stress, innate immune, and antioxidant systems, as well as indicators of energy mobilization, in pacu (Piaractus mesopotamicus) exposed to intermittent cold, aiming to assess the correlations between these responses. The fish were acclimated to 28 °C, divided into two groups, a control group maintained at 28 °C, and another exposed to 16 °C for two 24 h periods with a 5-day interval between them. The fish were sampled at six time points: baseline (after acclimatization to 28 °C), 24 h after the 1st exposure to 16 °C, after 5 days of recovery at 28 °C, 24 h after the 2nd exposure to 16 °C, and after 24 and 48 h of recovery at 28 °C. The reduction in temperature activated the stress response, the innate immune system, and the antioxidative system as well as mobilized lipids from the visceral stores and preserved the circulating levels of triglycerides. Intermittent exposure of fish to cold increased plasma cortisol after both exposures, activated leukocyte respiratory activity after the 2nd exposure, and triggered the compensatory lysozyme response after temperature recovery in both cold exposures in addition to increasing the number of circulating monocytes and granulocytes. The activity of the enzymes catalase (CAT) and superoxide dismutase (SOD) increased after the 1st and 2nd cold exposures, respectively. Glutathione peroxidase (GPx) activity increased after the 2nd exposure compared to the control. The subtropical fish pacu was sensitive to intermittent cold exposure and was able to display protective physiological responses.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"9"},"PeriodicalIF":2.5,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary purslane (Portulaca oleracea L.) leaf powder maintains growth and intestinal health in Oreochromis niloticus under chronic water-borne cadmium exposure by strengthening the gut barriers, modulating the intestinal nutrient transporters, and relieving oxidative stress.","authors":"Naief Dahran, Badriyah S Alotaibi, Yasmina M Abd-Elhakim, Amany Abdel-Rahman Mohamed, Rowida E Ibrahim, Mohamed M M Metwally, Tarek Khamis, Areej A Eskandrani, Manal E Alosaimi, Mohamed Y M Aly, Nouf A Babteen, Wafa S Alansari, Samah S Abuzahrah","doi":"10.1007/s10695-024-01415-5","DOIUrl":"10.1007/s10695-024-01415-5","url":null,"abstract":"<p><p>High cadmium (Cd) concentrations pose a threat to aquatic life globally. This study examined the efficiency of adding purslane (Portulaca oleracea L.) leaf powder (PLP) to Oreochromis niloticus diets on Cd's negative effects. PLP was analyzed using high-performance liquid chromatography, and its main constituents were gallic acid, chlorogenic acid, and pyrocatechol. Nile tilapia (180 fish, 34.5 ± 0.5 g) were divided into four groups in triplicate. A basal diet was given to the control group. The PLP group received a basal diet containing 10 g PLP/kg diet. The Cd group was exposed to 50 µg/L water. The Cd + PLP group was exposed to Cd and fed diets containing PLP. Results showed that PLP significantly rescued Cd-induced effects. PLP improved fish survival, feed conversion ratio, and growth retardation caused by Cd. PLP also restored decreased activities of lipase, trypsin, and amylase in the intestine. Furthermore, PLP corrected disturbances in leptin and growth hormone levels induced by Cd. PLP mitigated pathological alterations, replenished antioxidants (SOD, CAT, and GSH), and reduced lipid peroxidation in the intestinal tissues. PLP supplementation depleted significant Cd accumulation in the intestine and muscles. Additionally, PLP corrected altered expressions of tight junction proteins (zo-1, zo-2, and claudin-4) and nutrient transporters (glut-1, slc15a2, slc26a6, and slc4a4) in Cd-exposed fish. Conclusively, PLP shows promise as a dietary supplement to mitigate Cd's harmful impacts on fish growth. Its antioxidant activity and regulation of intestinal tight junction proteins and nutrient transporters contribute to its effectiveness. PLP supplementation holds the potential for reducing the detrimental effects of Cd in aquaculture.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"8"},"PeriodicalIF":2.5,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142964395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disruptive effects of genistein and daidzein on steroidogenesis, testicular development and maturation of Cyprinus carpio (Linn, 1758) males.","authors":"Vasanthakumaran Kumar, Pallath Muhammed Nuzaiba, Subodh Gupta, Subrata Dasgupta, Sunil Kumar Nayak, Arya Prabhakaran, Chandan Gowramma Mahadevaswamy, Atshaya Sundararajan, Sangeetha Sankar, Tincy Varghese","doi":"10.1007/s10695-024-01443-1","DOIUrl":"10.1007/s10695-024-01443-1","url":null,"abstract":"<p><p>A 60-day feeding trial was conducted to evaluate the combined effect of dietary soy phytoestrogens, specifically genistein and daidzein, on the gonadal recrudescence and maturation of male Cyprinus carpio (Linnaeus, 1758). Adult male C. carpio (60 ± 10 g) were fed with a diet with no added genistein or daidzein (C), 110 mg/100 mg genistein (GL), 210 mg/100 g genistein (GH), 4 mg/100 g daidzein (DL), 8 mg/100 g daidzein (DH), combination of 110 mg/100 mg genistein and 4 mg/100 g daidzein (DGL, equivalent to 17.5% soybean meal) and combination of 210 mg/100 g genistein and 8 mg/100 g daidzein (DGH, equivalent to 35% soybean meal) for 60 days. The fish were spent by injection of the inducing agent before the experiment to maintain the homogeneity in maturity stages. Serum 11-ketotestosterone and 17β-estradiol levels increased substantially (p < 0.05), while serum cortisol and 17α,20β dihydroxy progesterone levels were significantly (p < 0.05) lower in GH, DH, DL, DGL and DGH groups. The gene expression of cyp19a1a and 20β-HSD were significantly downregulated (p < 0.05) in GH, DH, and DGH. The ratio of spermatozoa to spermatocytes was lower in high doses of phytoestrogens and their combination treatments (GH, DH and DGH) than in the lower dose fed groups. The GSI (gonad somatic index) values showed a dose-dependent increase in the isoflavone-fed groups. Hence, the study concluded that the feeding of diets with a combination of 210 mg/100 g genistein and 8 mg/100 g daidzein (DGH), which is equivalent to 35% of soybean meal in the diet, disrupted the sex steroid profile and steroidogenic enzymes expression and caused a delay in maturation in the male common carp. Thus, soybean meal can be included in the diet of male common carp broodstock at levels up to 17.5%. However, inclusion levels above 35% have been shown to impair normal reproductive function.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"7"},"PeriodicalIF":2.5,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142947152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth performance, antioxidant, and immune responses of Nile tilapia (Oreochromis niloticus) fed on low-fishmeal diets enriched with sodium chloride and its adaptability to different salinity levels.","authors":"Mohamed N Monier, Asmaa S Abd El-Naby, Reham M Fawzy, Fatma Samir, Sherien H H Shady, Youssif Shehata Grana, Najah M Albaqami, Mohsen Abdel-Tawwab","doi":"10.1007/s10695-024-01426-2","DOIUrl":"10.1007/s10695-024-01426-2","url":null,"abstract":"<p><p>The current investigation assessed the beneficial impacts of dietary sodium chloride (NaCl) on the growth performance, oxidant/antioxidant, and immune responses of Nile tilapia (Oreochromis niloticus) and its adaptability to different salinity levels. After acclimating the fish to the laboratory conditions for 2 weeks, the acclimated fish (10.5 ± 0.16 g) were randomly distributed into 25 110-L rectangular glass tanks (15 fish/tank) to represent five groups in five replicates. The fish were fed with experimental feeds fortified with 0.0 (control), 5, 10, 15, and 20 g NaCl/kg feed for 60 days. Following the nutritional experiment, fish of all groups were adapted to different salinity levels from 0 to 32 g /L for a further 3 weeks, during which fish mortality was recorded. Blood samples were taken after the feeding trial and at a salinity level of 24 g/L. Growth performance and hematological parameters (WBCs, RBCs, hemoglobin, and hematocrit), total protein, albumin, globulin, digestive enzymes, antioxidant activity, and immunity status were markedly improved with increased NaCl rates in the fish diets up to 10 g/kg feed, after which all previous parameters were declined. On the other hand, feeding fish on a diet containing 10 g NaCl/kg feed showed substantially lower levels of cortisol, glucose, cholesterol, triglycerides, aspartate transaminase (AST), alanine transaminase (ALT), and malondialdehyde (MDA). Exposing the control fish group to salinity stress (32 g/L) for 3 weeks markedly decreased their digestive enzyme activity, immunity status, and antioxidant response, along with significant increases in cortisol, glucose, cholesterol, triglycerides, AST, ALT, and MDA levels. Conversely, feeding fish on a diet containing 10 g NaCl/kg feed alleviated the negative impacts of salinity stress and helped fish to tolerate salinity stress up to 24 g/L.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 1","pages":"6"},"PeriodicalIF":2.5,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142947153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}