Fish Physiology and Biochemistry最新文献

{"title":"Aluminum chloride (AlCl₃) alters the physiological response of rainbow trout.","authors":"Daniela Nualart, Daniela Diaz, Jaime Tapia, Carolina Quinteros, Luis Vargas-Chacoff","doi":"10.1007/s10695-025-01497-9","DOIUrl":"https://doi.org/10.1007/s10695-025-01497-9","url":null,"abstract":"<p><p>Fish production in terrestrial recirculating aquaculture systems (RAS) has steadily increased over the past decades. Therefore, several studies, both in freshwater and seawater, have shown that increased water reuse in RAS leads to increased levels of heavy metals in the bodies of fish. The objective of the present study was to evaluate the impact on the physiology, osmoregulatory mechanisms, and antioxidant response in fingerlings of rainbow trout (Oncorhynchus mykiss) exposed to varying doses of AlCl₃ within a recirculation system. Changes in gills, N⁺-K⁺-ATPase activity and H⁺-ATPase activity, plasma osmolality, lactate, glucose, chloride levels, and gills and liver oxidative stress responses were used to monitor physiology responses. As a result, AlCl₃ exposure modified responses in rainbow trout. Plasma parameters increased during the 6-day exposure, while lactate levels decreased in both doses. The enzymatic activities of antioxidant enzymes and the NKA pump increased when fish were exposed to high and low doses of aluminum chloride. Gene expression of glutathione reductase and peroxidase transcripts increased and decreased respectively in the liver, contrary to that observed in gill tissue. AlCl₃ was also observed in liver and gill tissues. This study shows that exposure to AlCl₃ affects ion regulation and oxidative stress in rainbow trout fry.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"84"},"PeriodicalIF":2.5,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response patterns and community assembly processes of gut microbiota in grass carp subjected to various protein sources and their implications for growth and metabolism.","authors":"Minglang Cai, Linlin Yang, Lei Zhong, Shouqi Xie, Yi Hu","doi":"10.1007/s10695-025-01498-8","DOIUrl":"https://doi.org/10.1007/s10695-025-01498-8","url":null,"abstract":"<p><p>Feed nutrients are crucial in shaping the gut microbial community, especially for complex interactions. While much research focused on the impacts of dietary protein levels, exploration of protein sources remains insufficient. Accordingly, this study specifically investigated the effects of four protein sources [Clostridium autoethanolicum protein (CAP), cottonseed protein concentrate (CPC), Chlorella vulgaris meal (CVP), and Tenebrio molitor meal (TM)] replacing dietary soybean meal on microbial co-occurrence networks and key metabolic taxa. A 56-day feeding trial involved 1500 grass carp (20.00 g) fed five experimental diets, each incorporating one of the experimental protein sources. Results revealed that CPC and CVP diets improved the weight gain and specific growth rate, with the CPC group demonstrating the highest biomass gain and the CVP group exhibiting the best feed conversion ratio. Findings further indicated that SM-free diets enhanced intestinal immunity and barrier function while negatively impacting microbial diversity. Additional profiling revealed that each treatment exhibited distinct abundance profiles and unique species, with Firmicutes, Bacteroidota, and Proteobacteria as the dominant phyla and key genera such as Bacteroides, Erysipelatoclostridium, and Cetobacterium. Stochastic mechanisms drove the community assembly process, and prolonged SM-free diets led to simplified networks with increased generalists and specialists. Functional gene analysis highlighted roles in amino acid, carbohydrate, and lipid metabolism, underscoring the impact of protein sources on aquatic microbial communities and host-microbiome interactions. Overall, the study suggests the potential suitability of several protein sources as soybean meal substitutes, emphasizing the importance of further investigation into optimal inclusion levels for diverse proteins.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"83"},"PeriodicalIF":2.5,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanozymes as a novel solution for oxidative stress reduction and nitrogen compound removal in aquatic systems: a study on common carp (Cyprinus carpio).","authors":"Ammar Abdulrazzaq Tawfeeq, Th T Mohammed, Bilal J M Aldahham","doi":"10.1007/s10695-025-01491-1","DOIUrl":"https://doi.org/10.1007/s10695-025-01491-1","url":null,"abstract":"<p><p>Antioxidants in fish are vital biomarkers when exposed to pollutants that cause oxidative stress. Among the most important components of the antioxidant defense system are the enzymes superoxide dismutase (SOD) and catalase (CAT). This study aimed to evaluate the potential of certain nanomaterials as nanozymes by mimicking the activity of SOD and CAT enzymes when added to the diet of common carp, simultaneously, removing the efficacy of nitrogen waste. To evaluate the effect of nanozymes on common carp fish, six types of experimental diets were used over 84 days. The study included six groups: a control group (T1) on a standard diet, and five groups supplemented with 1 mg kg⁻¹ of potassium dichromate (K₂Cr₂O₇). Among these, T3 and T4 also received 1 mg kg⁻¹ and 5 mg kg⁻¹ of CeO₂ nanoparticles, respectively, while T5 and T6 were given 1 mg kg⁻¹ and 5 mg kg⁻¹ of Fe₃O₄ nanoparticles, respectively. The experiment aimed to study the effects of K₂Cr₂O₇ combined with varying doses of CeO₂ and Fe₃O₄ nanoparticles. The results of the current study indicated a significant reduction (P ≤ 0.05) in certain nitrogenous compounds (ammonia and nitrates) in water in nanozyme treatments. The study also demonstrated a significant improvement (P ≤ 0.05) in productive performance in (T6), a significant decrease (P ≤ 0.05) in liver enzyme activity in blood serum in (T3), and a significant increase (P ≤ 0.05) in glutathione peroxidase (GPx) enzyme activity in (T5). Furthermore, there was a significant increase (P ≤ 0.05) in the gene expression of the enzymes SOD and CAT in (T4), as well as the gene expression of the GPx enzyme in (T6). The current study concludes that CeO₂ and Fe₃O₄ nanoparticles can be added to fish diets as nanozymes to combat oxidative stress in fish by mimicking the activities of the body's antioxidant enzymes. Nanozymes seem to reduce nitrogenous pollutants via adsorption or possibly oxidation.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 2","pages":"82"},"PeriodicalIF":2.5,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary propylene glycol mitigates cold stress and improves physio-metabolic responses in Pangasianodon hypophthalmus (Sauvage, 1878) fingerlings reared at low temperature.","authors":"Yash Khalasi, Ashutosh D Deo, Naseemashahul Shamna, Manish Jayant, Narottam Prasad Sahu, Akshaya Suresh, Ranju Kumari, Swaraj Adakney, Ashutosh Danve","doi":"10.1007/s10695-025-01493-z","DOIUrl":"https://doi.org/10.1007/s10695-025-01493-z","url":null,"abstract":"<p><p>A 60-day feeding trial was carried out to determine the effect of propylene glycol (PG) on feed intake and growth in Pangasianodon hypophthalmus (striped catfish) reared at low temperature (18.0 ± 1.0 °C) re-circulatory aquaculture system (RAS) with a flow rate of 1.6 L min^-1. Six isonitrogenous (370.0 g kg^-1) and isocaloric (17.22 MJ kg^-1 dietary energy) practical diets were designed and prepared with varying levels of PG, viz., control (0 ml kg^-1 PG), T_2.5 (2.5 ml kg^-1 PG), T_5.0 (5.0 ml kg^-1 PG), T_7.5 (7.5 ml kg^-1 PG), T_10.0 (10.0 ml kg^-1 PG), and T_12.5 (12.5 ml kg^-1 PG) diet. Fish were fed twice daily till satiation. The result showed that dietary supplementation of propylene glycol showed significantly higher feed intake in the treatment groups than in control (p < 0.05). The highest feed intake, growth rate, and feed conversion were observed in T_10.0 group (p < 0.05). However, these did not vary within T_2.5, T_5.0, and T_7.5 fed groups (p < 0.05). The highest protease and amylase activities were found in fish fed with 10.0 and 10.0-12.5 ml kg^-1 PG fed group (p < 0.05). However, lipase activity showed an increasing trend with the PG supplementation (p < 0.05). Metabolic enzymes and oxidative stress enzymes activities were highest in T_10.0 group fed with 10.0 ml kg^-1 PG. Serum total protein and albumin were also increased with PG supplementation and exhibited maximum values at 10.0 ml kg^-1 PG supplementation. These findings suggest that dietary supplementation with propylene glycol at a dose of 7.5-10.0 ml kg^-1 can improve feed intake, growth, feed conversion, and metabolic activities in P. hypophthalmus fingerlings raised at low temperature.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 2","pages":"81"},"PeriodicalIF":2.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the therapeutic potential of medicinal plants in zebrafish caudal fin regeneration and wound healing: a systematic review.","authors":"Zakariyya Muhammad Bello, Rosy Iara Maciel de Azambuja Ribeiro, Hélio Batista Dos Santos, Ralph Gruppi Thomé","doi":"10.1007/s10695-025-01495-x","DOIUrl":"https://doi.org/10.1007/s10695-025-01495-x","url":null,"abstract":"<p><p>This systematic review aims to examine the existing literature on the therapeutic potential of medicinal plants to improve caudal fin regeneration and wound healing in zebrafish (Danio rerio), focusing on uncovering their pharmacological properties and potential use in enhancing tissue repair and regeneration. A thorough review of suitable and eligible full-text articles was performed on PubMed, Scopus, Web of Science, and Google Scholar from 1 st January 2014 to 31 st December 2024. These articles were searched using the Medical Subject Headings terms \"zebrafish,\" \"zebrafish larvae,\" \"zebrafish embryo,\" \"angiogenesis,\" \"Medicinal plants,\" \"Natural products,\" \"Fin regeneration,\" \"wound healing,\" and \"inflammation.\" Here, 520 articles on medicinal plants and their potential in caudal fin regeneration and wound healing in zebrafish were identified across the databases searched, of which 26 were included in this study following screening. After thoroughly reviewing the articles, some were found to have used multiple medicinal plants. Thus, 38 medicinal plants were found to have promoted effects on zebrafish caudal fin regeneration and wound healing, and 21 revealed no effects on either caudal fin regeneration and wound healing. This systematic review explores the therapeutic potential of medicinal plants in caudal fin regeneration and wound healing in a zebrafish model. The results show a promising effect of various plant species in enhancing fin regeneration and wound healing. Further research is needed to understand the molecular mechanisms and to translate these findings into clinical applications for human wound healing and regenerative medicine.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 2","pages":"80"},"PeriodicalIF":2.5,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Milt androgen profile and evaluation of sperm morpho-functional characteristics of wild-caught and farmed European eels (Anguilla anguilla).","authors":"Laura Gentile, Bálint Lóránt Hausz, Antonio Casalini, Nadia Govoni, Pietro Emmanuele, Albamaria Parmeggiani, Domenico Ventrella, Maria Laura Bacci, Oliviero Mordenti, Alberto Elmi","doi":"10.1007/s10695-025-01494-y","DOIUrl":"10.1007/s10695-025-01494-y","url":null,"abstract":"<p><p>Evaluating milt samples before their preservation and/or reproductive use is critical to correctly develop assisted reproductive strategies for teleost species. This is especially true in case of \"critically endangered\" species, like the European eel (Anguilla anguilla), whose life cycle is not closed yet, leading to the necessity of capturing younglings from natural populations for both research and productive purposes. Despite androgens being involved in spermatogenesis and playing pivotal roles in the pre-migratory morphological and physiological changes, their quantification in different biological tissues is still to be refined in European eels. The aim of this study was to evaluate the feasibility of extraction and quantification of testosterone (T) and 11-ketotestosterone (11-KT) from male European eel milts. The secondary aim was to investigate potential differences in androgens' profiles and/or functional characteristics of spermatozoa between wild-caught and farmed specimens. Twenty-four male European eels obtained from the North Adriatic region (14 wild-caught and 10 farmed) underwent a maturation-inducing hormonal treatment for milt production; blood and milt samples were then collected for androgens quantification upon ELISA and RIA methodologies. Spermatozoa were also evaluated for viability and objective motility. Plasma showed average T and 11-KT levels of 2.32 and 6.88 ng/mL, respectively, while milt 0.24 (T) and 1.09 ng/mL (11-KT). Significantly higher spermatozoa concentrations and motility were observed in farmed eels' milts. This work contributes to deepening the knowledge on teleost physiology, being the first report quantifying T and 11-KT from European eels' seminal plasma and comparing the quality of milts of farmed vs wild specimens.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 2","pages":"78"},"PeriodicalIF":2.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11976806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis unveils adaptation strategies in silver pomfret (Pampus argenteus) gills under hypoxic conditions.","authors":"Fei Zhu, Meixuan Lei, Yufeng Song, Dafeng Xu, Shuran Du, Qian Meng, Chaofeng Jia, Shaowu Yin, Shuyin Chen, Cheng Zhao","doi":"10.1007/s10695-025-01492-0","DOIUrl":"10.1007/s10695-025-01492-0","url":null,"abstract":"<p><p>The silver pomfret (Pampus argenteus) is an economically valuable and highly sought-after table fish in China. In recent years, commercial farming of silver pomfret has been successfully developed in the country. However, silver pomfret is highly sensitive to environmental stress, and hypoxia-induced stress can lead to significant economic losses in aquaculture. This study investigated the transcriptomic response of silver pomfret gills under normal oxygen conditions (G0) and after hypoxic exposure for 6 h (G6) and 24 h (G24). Hypoxia exposure induced gill remodeling, characterized by increased gill lamellar height and a reduction in interlamellar cell mass (ILCM). Oxidative stress and antioxidant responses were significantly upregulated after 24 h of hypoxia exposure. Additionally, many downregulated genes were significantly enriched in pathways related to cardiac muscle contraction and calcium signaling, leading to impaired gill musculature contraction and reduced oxygen uptake under hypoxic conditions. Key signal transduction pathways, including HIF- 1, Apelin, and MAPK signaling, were identified as critical pathways in response to hypoxia. Furthermore, hypoxia tended to suppress the immune system and disrupted endoplasmic reticulum homeostasis and protein processing in the gills of silver pomfret. In summary, this study demonstrates that hypoxia disrupts gill function in silver pomfret and provides insights into hypoxia adaptation mechanisms in teleosts.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 2","pages":"79"},"PeriodicalIF":2.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Releasing stored lipids to fuel migration and reproduction in the eel, Anguilla australis-a role for 11-ketotestosterone?","authors":"P Mark Lokman, Deborah Lynch, Peter S Davie, Erin L Damsteegt","doi":"10.1007/s10695-025-01480-4","DOIUrl":"10.1007/s10695-025-01480-4","url":null,"abstract":"<p><p>Migrating freshwater eels depend on the mobilisation of stored lipids to successfully arrive at their distant spawning locations. As 11-ketotestosterone (11KT) can increase the lipid-transporting capability and enhance gonadal lipid uptake in eel, we hypothesized that this androgen would also regulate lipid mobilisation from its stores. To address this hypothesis, we first sampled residential (yellow) and migrating (silver) short-finned eels from the wild and evaluated the expression of 24 genes encoding lipolytic or lipogenic enzymes, as well as those encoding both nuclear androgen receptors, by NanoString analysis. Plasma 11KT levels in silver eels were dramatically increased, and mRNA levels of more than half of all target genes were higher in silver eel muscle; none of the target genes was significantly downregulated. Gene expression profiles in white muscle from wild-caught eels were subsequently compared with those from yellow and silver eels subjected to implantation with sustained-release implants containing 11KT. Several weeks of exposure resulted in plasma levels of 11KT that resembled those of wild-caught eels and resulted in a dose-dependent increase in gonadosomatic and hepatosomatic index; however, target gene expression profiles in muscle were barely affected. We conclude that lipid physiology in white muscle of silver eels is notably different from that in yellow eels, and that 11KT is not responsible for the differentially expressed gene profile between yellow and silver short-finned eels.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 2","pages":"77"},"PeriodicalIF":2.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11976764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ability of dietary essential oils to mitigate nickel-induced growth retardation, immune-antioxidant suppression, and endoplasmic reticulum stress activation in Nile tilapia.","authors":"Shaimaa A A Ahmed, Ghada I Abd El-Rahman, Haiam A Mohammed, Samar A Abdo, Mohamed Y M Aly, Hala Elshahat Ghannam, Fatma Mahsoub, Tarek Khamis, Rowida E Ibrahim","doi":"10.1007/s10695-025-01482-2","DOIUrl":"10.1007/s10695-025-01482-2","url":null,"abstract":"<p><p>Fish immunity is significantly impacted by waterborne metal intoxication. Nickel is a ubiquitous metal in aquatic bodies which badly impacts fish immune responses and survival. In the current research, we looked into the possible protective effects of essential oils of tea tree (TTO) and basil (BEO) against nickel exposure in Nile tilapia, or Oreochromis niloticus. To achieve this, 240 Nile tilapia (27.92 ± 0.22 g) were categorized into six groups for 45 days; three groups were fed on basal control, TTO, and BEO diets without Ni exposure. The other three groups were subjected to Ni at a level of 3.6 mg/L and fed on basal, TTO, and BEO diets. The outcomes showed a substantial decrease in the growth measures and survival % in the Ni-exposed fish. Oxidative stress (higher splenic malondialdehyde and lower splenic catalase, reduced glutathione, and superoxide dismutase), immune suppression (lower serum lysozyme, myeloperoxidase, and nitric oxide), and elevated serum cortisol levels were observed in the Ni-intoxicated group. The Ni-intoxicated group exhibited various pathological alterations detected in the intestinal tissue mainly inflammatory and necrotic changes, moreover, moderate depletion of lymphoid elements represented by necrotic lymphocytes in the splenic tissue. Ni-exposure induced endoplasmic reticulum stress through up-regulation of the splenic expression of protein kinase R-like endoplasmic reticulum kinase, activating transcription factor 6, CCAAT/enhancer-binding protein homologous protein, X-box binding protein 1, α-subunit of eukaryotic initiation factor 2, inositol-requiring kinase 1a, mitogen-activated protein kinase, c-JunN-terminal kinase, and binding protein for immunoglobulins. Feeding on the TTO and BEO diets improved the growth measures, survival, and antioxidant capacity. Immunomodulation, enhancement of the intestinal and splenic architecture, and relief of the endoplasmic reticulum stress condition were noticed when the Ni-intoxicant groups were fed on TTO and BEO diets. Overall, the effect of the TTO diet was more pronounced than the BEO diet in mitigating the negative consequences of Ni-intoxication in Nile tilapia. Dietary fortification of 0.1% TTO and/or 0.1% BEO is recommended during Ni exposure in aquaculture practices.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 2","pages":"76"},"PeriodicalIF":2.5,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11958502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generating transcriptomic resources in the teleost fish black cusk-eel (Genypterus maculatus) to evaluate thermal stress in the liver under a climate change scenario.","authors":"Phillip Dettleff, Marcia Fuentes, Pamela Gonzalez, Jorge Aedo, Rodrigo Zuloaga, Juan Manuel Estrada, Alfredo Molina, Juan Antonio Valdes","doi":"10.1007/s10695-025-01486-y","DOIUrl":"10.1007/s10695-025-01486-y","url":null,"abstract":"<p><p>The black cusk-eel (Genypterus maculatus) is a native fish that is relevant for traditional Chilean fisheries and has aquaculture potential. However, the genomic information and the biological knowledge related to the effect of thermal stress response are limited. This study generated the first de novo transcriptome assembly of the liver of G. maculatus and investigated the hepatic response to thermal stress in the G. maculatus. The de novo assembly resulted in 26,620 annotated transcripts, with an N50 of 2297, and a GC% of 49.76%. BUSCO analysis showed 97.1% and 75.7% complete orthologous (Metazoa and Actinopterygi, respectively). Functional annotation showed a total of 55,556 GO terms, with 26,128 annotations on biological process, 15,225 annotations on molecular functions, and 14,213 annotations on cellular component. The RNA-seq analysis revealed 94 differentially expressed transcripts in response to thermal stress, with 64 downregulated and 30 upregulated transcripts. The enrichment analysis showed biological processes related to double-strand break repair via homologous recombination, reciprocal meiotic recombination, and DNA repair. A significant increase in cortisol levels with no significant difference activity of hepatic enzymes (ALT, AST, AP) due to thermal stress was observed. Also, an increase in DNA damage (AP sites formation) and lipid peroxidation (HNE protein adducts) in the liver due to thermal stress was observed. The differentially expressed transcripts were validated using qPCR, confirming the RNA-seq results. The findings provide valuable genomic information for G. maculatus and highlight the physiological and molecular responses to thermal stress in the species under the context of climate change.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 2","pages":"75"},"PeriodicalIF":2.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}