FEMS immunology and medical microbiology最新文献

Structural, serological, and genetic characterization of the O-antigen of Providencia alcalifaciens O40. 碱性普罗维登氏菌O40 o抗原的结构、血清学和遗传学特征。

FEMS immunology and medical microbiology Pub Date : 2012-12-01 DOI: 10.1111/1574-695X.12002

Olga G Ovchinnikova, Bin Liu, Dan Guo, Nina A Kocharova, Magdalena Bialczak-Kokot, Alexander S Shashkov, Lu Feng, Antoni Rozalski, Lei Wang, Yuriy A Knirel

{"title":"Structural, serological, and genetic characterization of the O-antigen of Providencia alcalifaciens O40.","authors":"Olga G Ovchinnikova, Bin Liu, Dan Guo, Nina A Kocharova, Magdalena Bialczak-Kokot, Alexander S Shashkov, Lu Feng, Antoni Rozalski, Lei Wang, Yuriy A Knirel","doi":"10.1111/1574-695X.12002","DOIUrl":"https://doi.org/10.1111/1574-695X.12002","url":null,"abstract":"The O-polysaccharide chain of the lipopolysaccharide (O-antigen) on the bacterial cell surface is one of the most structurally variable cell components and serves as a basis for serotyping of Gram-negative bacteria, including human opportunistic pathogens of the genus Providencia. In this work, the O-antigen of Providencia alcalifaciens O40 was obtained by mild acid degradation of the isolated lipopolysaccharide and studied by chemical methods and high-resolution NMR spectroscopy. The following structure of the O-polysaccharide was established: →4)-β-D-Quip3NFo-(1→3)-α-D-Galp-(1→3)-β-D-GlcpA-(1→3)-β-D-GalpNAc-(1→, where GlcA stands for glucuronic acid and Qui3NFo for 3,6-dideoxy-3-formamidoglucose. The O40-antigen was found to be structurally and serologically related to the O-antigens of P. alcalifaciens O5 and Providencia stuartii O18. The O40-antigen gene cluster between cpxA and yibK was sequenced, and the gene functions were predicted in silico. In agreement with the O-polysaccharide structure established, the genes for the synthesis of dTDP-D-Qui3NFo, UDP-D-Gal, UDP-D-GlcA, and UDP-D-GalNAc as well as those encoding three glycosyltransferases, flippase (Wzx), and O-antigen polymerase (Wzy) were recognized. In addition, homologues of wza, wzb, and wzc genes, which are required for the surface expression of capsular polysaccharides, were found within the gene cluster, suggesting that the O-polysaccharide studied is a part of the capsule-related form of the lipopolysaccharide called K(LPS).","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"66 3","pages":"382-92"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/1574-695X.12002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31058183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Molecular characteristics of community-acquired, methicillin-resistant Staphylococcus aureus isolated from Chinese children. 中国儿童社区获得性耐甲氧西林金黄色葡萄球菌的分子特征。

FEMS immunology and medical microbiology Pub Date : 2010-04-01 Epub Date: 2010-01-28 DOI: 10.1111/j.1574-695X.2010.00648.x

Wenjing Geng, Yonghong Yang, Dejing Wu, Guoying Huang, Chuanqing Wang, Li Deng, Yuejie Zheng, Zhou Fu, Changcong Li, Yunxiao Shang, Changan Zhao, Sangjie Yu, Xuzhuang Shen

{"title":"Molecular characteristics of community-acquired, methicillin-resistant Staphylococcus aureus isolated from Chinese children.","authors":"Wenjing Geng, Yonghong Yang, Dejing Wu, Guoying Huang, Chuanqing Wang, Li Deng, Yuejie Zheng, Zhou Fu, Changcong Li, Yunxiao Shang, Changan Zhao, Sangjie Yu, Xuzhuang Shen","doi":"10.1111/j.1574-695X.2010.00648.x","DOIUrl":"https://doi.org/10.1111/j.1574-695X.2010.00648.x","url":null,"abstract":"The aim of this study was to investigate the molecular characteristics of community-acquired, methicillin-resistant Staphylococcus aureus (CA-MRSA) isolates from Chinese children. Ninety-nine isolates were collected from eight hospitals, and analyzed by multilocus sequence typing, staphylococcal chromosomal cassette mec (SCCmec) type, and spa typing. The Panton-Valentine leukocidin (PVL) gene was also detected. Overall, 14 sequence types (STs) were obtained, and ST59 (58.6%) was found to be the most prevalent, followed by ST1 (8%) and ST338 (8%). We also first registered the new ST1409. SCCmec type IV was the most predominant type at 67.7%, followed by SCCmec type V at 32.3%. SCCmec subtypes IVa, IVc, and IVg were found among the SCCmec type IV strains. Twenty-one spa types were also identified. Four new spa types were found by synchronization with the Ridom SpaServer and referring to the website (http://www.SeqNet.org). ST59-MRSA-IVa with t437 accounted for 40.4% of occurrences, making it the most prevalent clone. The prevalence of PVL genes was 58.6%, and multidrug resistance was observed in 95% of all isolates. This result indicates that CA-MRSA isolates in Chinese children are largely associated with the ST59-MRSA-IV clone, and that the predominant clones of CA-MRSA are spread all over the country.","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"58 3","pages":"356-62"},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1574-695X.2010.00648.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28693933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 56

Autoactivation of the AggR regulator of enteroaggregative Escherichia coli in vitro and in vivo. 体外和体内肠道聚集性大肠杆菌AggR调节因子的自激活。

FEMS immunology and medical microbiology Pub Date : 2010-04-01 Epub Date: 2010-01-28 DOI: 10.1111/j.1574-695X.2010.00645.x

Nicholas Morin, Chelsea Tirling, Sabine M Ivison, Ajinder Pal Kaur, James P Nataro, Theodore S Steiner

{"title":"Autoactivation of the AggR regulator of enteroaggregative Escherichia coli in vitro and in vivo.","authors":"Nicholas Morin, Chelsea Tirling, Sabine M Ivison, Ajinder Pal Kaur, James P Nataro, Theodore S Steiner","doi":"10.1111/j.1574-695X.2010.00645.x","DOIUrl":"https://doi.org/10.1111/j.1574-695X.2010.00645.x","url":null,"abstract":"Enteroaggregative Escherichia coli (EAEC) causes diarrhea in diverse populations worldwide. The AraC-like regulator AggR is a key virulence regulator in EAEC. AggR-regulated genes include those encoding the Aggregative Adherence Fimbria, the dispersin protein, and a type VI secretion system. This study characterizes the regulation of the aggR promoter (P(aggR)). Using primer extension analysis, the transcriptional start site of the aggR promoter was located 40 nucleotides upstream of the translational start. P(aggR) was found to be autoregulated and DNA footprinting revealed the presence of two AggR-binding sites: one upstream of the transcriptional start site and one downstream. Additionally, P(aggR) was found to be positively regulated by the DNA-binding protein FIS and negatively regulated by the global regulator H-NS. To further understand this complex regulation scheme, a bacterial luciferase reporter system was used with a mouse model of EAEC colonization. This allowed for the in vivo measurement of P(aggR), P(fis), and P(hns) activity. EAEC present in the mouse intestine possessed relatively high levels of P(fis) and P(aggR) activity and a low level of P(hns) when compared with in vitro experiments. The data provide significant insights into the regulation cascade leading to aggR expression in the mammalian intestine during EAEC infection.","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"58 3","pages":"344-55"},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1574-695X.2010.00645.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28693935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 44

Development and evaluation of loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Penicillium marneffei in archived tissue samples. 环介导等温扩增(LAMP)快速诊断存档组织标本中马尔尼菲青霉的建立与评价。

FEMS immunology and medical microbiology Pub Date : 2010-04-01 Epub Date: 2010-01-26 DOI: 10.1111/j.1574-695X.2010.00647.x

Jiufeng Sun, Xiqing Li, Hanxiang Zeng, Zhi Xie, Changming Lu, Liyan Xi, Gert S de Hoog

{"title":"Development and evaluation of loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Penicillium marneffei in archived tissue samples.","authors":"Jiufeng Sun, Xiqing Li, Hanxiang Zeng, Zhi Xie, Changming Lu, Liyan Xi, Gert S de Hoog","doi":"10.1111/j.1574-695X.2010.00647.x","DOIUrl":"https://doi.org/10.1111/j.1574-695X.2010.00647.x","url":null,"abstract":"Penicillium marneffei is the etiologic agent of a severe systemic disease in immunocompromised hosts in Southeast Asia. In the present study, a novel method, known as loop-mediated isothermal amplification (LAMP), is described for the rapid and specific detection of the species, using a primer set derived from the internal transcribed spacer (ITS) region of the rRNA gene. Amplification products can be detected macroscopically by visual inspection in vials using SYBR Green I as well as by electrophoresis on agarose gel. The LAMP assay resulted in specific amplification of P. marneffei ITS using pure cultures after a 1-h reaction at 65 degrees C in a water bath; no cross-reactivity with other fungi including other biverticillate penicillia was observed. The detectable DNA limit was two copies. In addition, specific amplification was achieved using paraffin wax-embedded tissue samples from patients with penicilliosis marneffei and tissue samples from bamboo rats. The method provides a powerful tool for rapid diagnostics in the clinical lab, and has potential for use in ecological studies.","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"58 3","pages":"381-8"},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1574-695X.2010.00647.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28678938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaccination prevents Helicobacter pylori-induced alterations of the gastric flora in mice. 疫苗可预防幽门螺杆菌引起的小鼠胃菌群的改变。

FEMS immunology and medical microbiology Pub Date : 2006-03-01 DOI: 10.1111/j.1574-695X.2005.00024.x

Toni Aebischer, André Fischer, Anna Walduck, Cord Schlötelburg, Mirko Lindig, Sören Schreiber, Thomas F Meyer, Stefan Bereswill, Ulf B Göbel

{"title":"Vaccination prevents Helicobacter pylori-induced alterations of the gastric flora in mice.","authors":"Toni Aebischer, André Fischer, Anna Walduck, Cord Schlötelburg, Mirko Lindig, Sören Schreiber, Thomas F Meyer, Stefan Bereswill, Ulf B Göbel","doi":"10.1111/j.1574-695X.2005.00024.x","DOIUrl":"https://doi.org/10.1111/j.1574-695X.2005.00024.x","url":null,"abstract":"Molecular analysis of the gastric microflora in mice revealed that Helicobacter pylori infection causes an increase in microbial diversity. The stomachs of H. pylori-infected animals were colonized by bacteria which are naturally restricted to the lower intestinal tract. Clostridia, Bacteroides/Prevotella spp., Eubacterium spp., Ruminococcus spp., streptococci and Escherichia coli were detected exclusively in the stomachs of infected animals, whereas lactobacilli dominated the gastric flora in noninfected mice. The H. pylori-induced shifts in the gastric microbiota were independent from histological pathology and from changes in the gastric pH but were prevented by immunization of mice with live Salmonella expressing H. pylori urease. Immunized mice displayed reduced H. pylori levels in the gastric epithelium and developed a normal gastric microflora, indicating that vaccination may be protective against H. pylori-induced changes in the gastric flora.","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"46 2","pages":"221-9"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1574-695X.2005.00024.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25864037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

HIV Chemotherapy: A Critical Review HIV化疗:一个重要的回顾

FEMS immunology and medical microbiology Pub Date : 2006-02-01 DOI: 10.1111/J.1574-695X.2005.00042.X

A. Belkum

引用次数: 2

Coccidioidomycosis and blastomycosis: Advances in molecular diagnosis 球孢子菌病和芽孢菌病:分子诊断的进展

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/j.femsim.2005.05.011

Ralf Bialek , Gloria M. González , Dominik Begerow , Ulrike E. Zelck

引用次数: 40

Editorial: Molecular genetic approaches to the study of human pathogenic fungi 社论:人类致病真菌研究的分子遗传学方法

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/j.femsim.2005.06.004

George S. Kobayashi (Guest editors), Maria Lucia Taylor, Eduardo Dei-Cas

引用次数: 0

Electrophoresis karyotype and chromosome-length polymorphism of Histoplasma capsulatum clinical isolates from Latin America 拉丁美洲荚膜组织浆体临床分离株的电泳核型和染色体长度多态性

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/j.femsim.2005.05.015

Cristina Elena Canteros , María Fernanda Zuiani , Viviana Ritacco , Diego E. Perrotta , María Rocío Reyes-Montes , Julio Granados , Gerardo Zúñiga , Maria Lucia Taylor , Graciela Davel

引用次数: 12

Identification of the infectious source of an unusual outbreak of histoplasmosis, in a hotel in Acapulco, state of Guerrero, Mexico 在墨西哥格雷罗州阿卡普尔科的一家酒店内，组织胞浆菌病异常暴发的传染源鉴定

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/j.femsim.2005.05.017

Maria Lucia Taylor , Guillermo M. Ruíz-Palacios , María del Rocío Reyes-Montes , Gabriela Rodríguez-Arellanes , Laura E. Carreto-Binaghi , Esperanza Duarte-Escalante , Aurora Hernández-Ramírez , Armando Pérez , Roberto O. Suárez-Alvarez , Yuri A. Roldán-Aragón , Rafael Romero-Martínez , Jorge H. Sahaza-Cardona , José Sifuentes-Osornio , Luis E. Soto-Ramírez , Gabriela R. Peña-Sandoval

{"title":"Identification of the infectious source of an unusual outbreak of histoplasmosis, in a hotel in Acapulco, state of Guerrero, Mexico","authors":"Maria Lucia Taylor , Guillermo M. Ruíz-Palacios , María del Rocío Reyes-Montes , Gabriela Rodríguez-Arellanes , Laura E. Carreto-Binaghi , Esperanza Duarte-Escalante , Aurora Hernández-Ramírez , Armando Pérez , Roberto O. Suárez-Alvarez , Yuri A. Roldán-Aragón , Rafael Romero-Martínez , Jorge H. Sahaza-Cardona , José Sifuentes-Osornio , Luis E. Soto-Ramírez , Gabriela R. Peña-Sandoval","doi":"10.1016/j.femsim.2005.05.017","DOIUrl":"10.1016/j.femsim.2005.05.017","url":null,"abstract":"<div>Three isolates of Histoplasma capsulatum were identified from mice lung, liver, and spleen inoculated with soil samples of the X hotel’s ornamental potted plants that had been fertilized with organic material known as compost. The presence of H. capsulatum in the original compost was detected using the dot-enzyme-linked immunosorbent assay. Nested-PCR, using a specific protein Hcp100 coding gene sequence, confirmed the fungal identification associated with an unusual histoplasmosis outbreak in Acapulco. Although, diversity between the H. capsulatum isolate from the hotel and some clinical isolates from Guerrero (positive controls) was observed using random amplification of polymorphic DNA based-PCR, sequence analyses of H-anti and ole fragment genes revealed a high homology (92–99%) between them.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 3","pages":"Pages 435-441"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25225740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40