Vaccination prevents Helicobacter pylori-induced alterations of the gastric flora in mice.

Toni Aebischer, André Fischer, Anna Walduck, Cord Schlötelburg, Mirko Lindig, Sören Schreiber, Thomas F Meyer, Stefan Bereswill, Ulf B Göbel
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引用次数: 28

Abstract

Molecular analysis of the gastric microflora in mice revealed that Helicobacter pylori infection causes an increase in microbial diversity. The stomachs of H. pylori-infected animals were colonized by bacteria which are naturally restricted to the lower intestinal tract. Clostridia, Bacteroides/Prevotella spp., Eubacterium spp., Ruminococcus spp., streptococci and Escherichia coli were detected exclusively in the stomachs of infected animals, whereas lactobacilli dominated the gastric flora in noninfected mice. The H. pylori-induced shifts in the gastric microbiota were independent from histological pathology and from changes in the gastric pH but were prevented by immunization of mice with live Salmonella expressing H. pylori urease. Immunized mice displayed reduced H. pylori levels in the gastric epithelium and developed a normal gastric microflora, indicating that vaccination may be protective against H. pylori-induced changes in the gastric flora.

疫苗可预防幽门螺杆菌引起的小鼠胃菌群的改变。
对小鼠胃微生物区系的分子分析表明幽门螺杆菌感染可引起微生物多样性的增加。幽门螺旋杆菌感染动物的胃被细菌定植,这些细菌自然局限于下肠道。感染动物胃中仅检出梭状芽孢杆菌、拟杆菌/普氏菌、真杆菌、瘤胃球菌、链球菌和大肠杆菌,而未感染小鼠胃中主要检出乳酸菌。幽门螺杆菌引起的胃微生物群的变化与组织学病理和胃pH的变化无关,但可以通过表达幽门螺杆菌脲酶的活沙门氏菌免疫小鼠来预防。免疫小鼠显示胃上皮中幽门螺杆菌水平降低,并形成正常的胃菌群,这表明接种疫苗可能对幽门螺杆菌引起的胃菌群变化具有保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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