Cristina Elena Canteros , María Fernanda Zuiani , Viviana Ritacco , Diego E. Perrotta , María Rocío Reyes-Montes , Julio Granados , Gerardo Zúñiga , Maria Lucia Taylor , Graciela Davel
{"title":"拉丁美洲荚膜组织浆体临床分离株的电泳核型和染色体长度多态性","authors":"Cristina Elena Canteros , María Fernanda Zuiani , Viviana Ritacco , Diego E. Perrotta , María Rocío Reyes-Montes , Julio Granados , Gerardo Zúñiga , Maria Lucia Taylor , Graciela Davel","doi":"10.1016/j.femsim.2005.05.015","DOIUrl":null,"url":null,"abstract":"<div><p>Intact chromosomes of 19 clinical isolates of <span><em>Histoplasma capsulatum</em></span> recently obtained in Argentina, Mexico and Guatemala and the laboratory reference strain G186B from Panama were analyzed using pulsed-field gel electrophoresis. Chromosomal banding patterns of the human isolates revealed 5–7 bands, ranging from 1.3 to 10<!--> <!-->Mbp in size. Strain G186B showed five bands of approximately 1.1, 2.8, 3.3, 5.4 and 9.7<!--> <!-->Mbp. Thirteen different electrokaryotypes were identified, indicating that the genome of <em>H. capsulatum</em> varies widely in nature, as observed previously in laboratory strains. No definite association was found between electrokaryotype and geographical or clinical source.</p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.015","citationCount":"12","resultStr":"{\"title\":\"Electrophoresis karyotype and chromosome-length polymorphism of Histoplasma capsulatum clinical isolates from Latin America\",\"authors\":\"Cristina Elena Canteros , María Fernanda Zuiani , Viviana Ritacco , Diego E. Perrotta , María Rocío Reyes-Montes , Julio Granados , Gerardo Zúñiga , Maria Lucia Taylor , Graciela Davel\",\"doi\":\"10.1016/j.femsim.2005.05.015\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Intact chromosomes of 19 clinical isolates of <span><em>Histoplasma capsulatum</em></span> recently obtained in Argentina, Mexico and Guatemala and the laboratory reference strain G186B from Panama were analyzed using pulsed-field gel electrophoresis. Chromosomal banding patterns of the human isolates revealed 5–7 bands, ranging from 1.3 to 10<!--> <!-->Mbp in size. Strain G186B showed five bands of approximately 1.1, 2.8, 3.3, 5.4 and 9.7<!--> <!-->Mbp. Thirteen different electrokaryotypes were identified, indicating that the genome of <em>H. capsulatum</em> varies widely in nature, as observed previously in laboratory strains. No definite association was found between electrokaryotype and geographical or clinical source.</p></div>\",\"PeriodicalId\":12220,\"journal\":{\"name\":\"FEMS immunology and medical microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.015\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"FEMS immunology and medical microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0928824405001513\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEMS immunology and medical microbiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0928824405001513","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Electrophoresis karyotype and chromosome-length polymorphism of Histoplasma capsulatum clinical isolates from Latin America
Intact chromosomes of 19 clinical isolates of Histoplasma capsulatum recently obtained in Argentina, Mexico and Guatemala and the laboratory reference strain G186B from Panama were analyzed using pulsed-field gel electrophoresis. Chromosomal banding patterns of the human isolates revealed 5–7 bands, ranging from 1.3 to 10 Mbp in size. Strain G186B showed five bands of approximately 1.1, 2.8, 3.3, 5.4 and 9.7 Mbp. Thirteen different electrokaryotypes were identified, indicating that the genome of H. capsulatum varies widely in nature, as observed previously in laboratory strains. No definite association was found between electrokaryotype and geographical or clinical source.
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