拉丁美洲荚膜组织浆体临床分离株的电泳核型和染色体长度多态性

Cristina Elena Canteros , María Fernanda Zuiani , Viviana Ritacco , Diego E. Perrotta , María Rocío Reyes-Montes , Julio Granados , Gerardo Zúñiga , Maria Lucia Taylor , Graciela Davel
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引用次数: 12

摘要

采用脉冲场凝胶电泳技术对阿根廷、墨西哥和危地马拉19株荚膜组织浆菌临床分离株和巴拿马实验室参考菌株G186B的完整染色体进行了分析。人分离株的染色体带型为5 ~ 7条,大小为1.3 ~ 10 Mbp。菌株G186B显示出约1.1、2.8、3.3、5.4和9.7 Mbp的5条条带。鉴定出13种不同的电核型,表明荚膜孢子虫的基因组在自然界中差异很大,正如以前在实验室菌株中观察到的那样。电核型与地理或临床来源之间没有明确的联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Electrophoresis karyotype and chromosome-length polymorphism of Histoplasma capsulatum clinical isolates from Latin America

Intact chromosomes of 19 clinical isolates of Histoplasma capsulatum recently obtained in Argentina, Mexico and Guatemala and the laboratory reference strain G186B from Panama were analyzed using pulsed-field gel electrophoresis. Chromosomal banding patterns of the human isolates revealed 5–7 bands, ranging from 1.3 to 10 Mbp in size. Strain G186B showed five bands of approximately 1.1, 2.8, 3.3, 5.4 and 9.7 Mbp. Thirteen different electrokaryotypes were identified, indicating that the genome of H. capsulatum varies widely in nature, as observed previously in laboratory strains. No definite association was found between electrokaryotype and geographical or clinical source.

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