FEMS immunology and medical microbiology最新文献_第2页

Manganese superoxide dismutase in pathogenic fungi: An issue with pathophysiological and phylogenetic involvements 致病真菌中的锰超氧化物歧化酶:一个涉及病理生理和系统发育的问题

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/j.femsim.2005.06.003

Emilie Fréalle , Christophe Noël , Eric Viscogliosi , Daniel Camus , Eduardo Dei-Cas , Laurence Delhaes

{"title":"Manganese superoxide dismutase in pathogenic fungi: An issue with pathophysiological and phylogenetic involvements","authors":"Emilie Fréalle , Christophe Noël , Eric Viscogliosi , Daniel Camus , Eduardo Dei-Cas , Laurence Delhaes","doi":"10.1016/j.femsim.2005.06.003","DOIUrl":"10.1016/j.femsim.2005.06.003","url":null,"abstract":"<div>Manganese-containing superoxide dismutases (MnSODs) are ubiquitous metalloenzymes involved in cell defence against endogenous and exogenous reactive oxygen species. In fungi, using this essential enzyme for phylogenetic analysis of Pneumocystis and Ganoderma genera, and of species selected among Ascomycota, Basidiomycota and Zygomycota, provided interesting results in taxonomy and evolution. The role of mitochondrial and cytosolic MnSODs was explored in some pathogenic Basidiomycota yeasts (Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. gattii, Malassezia sympodialis), Ascomycota filamentous fungi (Aspergillus fumigatus), and Ascomycota yeasts (Candida albicans). MnSOD-based phylogenetic and pathogenic data are confronted in order to evaluate the roles of fungal MnSODs in pathophysiological mechanisms.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 3","pages":"Pages 411-422"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.06.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25220354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Analysis and expression of STE13ca gene encoding a putative X-prolyl dipeptidyl aminopeptidase from Candida albicans 白色念珠菌x -脯氨酸二肽基氨基肽酶编码基因STE13ca的分析与表达

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/j.femsim.2005.05.020

Consuelo Bautista-Muñoz, César Hernández-Rodrı́guez, Lourdes Villa-Tanaca

{"title":"Analysis and expression of STE13ca gene encoding a putative X-prolyl dipeptidyl aminopeptidase from Candida albicans","authors":"Consuelo Bautista-Muñoz, César Hernández-Rodrı́guez, Lourdes Villa-Tanaca","doi":"10.1016/j.femsim.2005.05.020","DOIUrl":"10.1016/j.femsim.2005.05.020","url":null,"abstract":"<div>Candida albicans STE13ca gene was identified by its homology to the Saccharomyces cerevisiae STE13 gene that encodes for the dipeptidyl aminopeptidase A (DAP A) involved in the maturation of α-factor mating pheromone. Our study revealed that C. albicans ATCC 10231 depicts dipeptidyl aminopeptidase activity. We also analyzed the expression of the STE13ca gene homologue from this pathogenic yeast. This gene of 2793pb is homozygotic and encodes for a predicted protein of 930 amino acids with a molecular weight of 107,035Da. The predicted protein displays significant sequence similarity to S. cerevisiae Ste13p. This C. albicans gene is located in chromosome R. STE13ca gene increases its levels of expression in conditions of nutritional stress (proline as nitrogen source) and during formation of the germinal tube, suggesting a basic biological function for the STE13ca in this yeast.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 3","pages":"Pages 459-469"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25222152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Cryptococcus neoformans: A sugar-coated killer with designer genes 新生隐球菌:一种带有设计基因的糖衣杀手

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/j.femsim.2005.06.005

John R. Perfect

{"title":"Cryptococcus neoformans: A sugar-coated killer with designer genes","authors":"John R. Perfect","doi":"10.1016/j.femsim.2005.06.005","DOIUrl":"10.1016/j.femsim.2005.06.005","url":null,"abstract":"<div>Cryptococcus neoformans has become a common central nervous system pathogen as the immunocompromised populations enlarge world-wide. This encapsulated yeast has significant advantages for the study of fungal pathogenesis and these include: (1) a clinically important human pathogen; (2) a tractable genetic system; (3) advanced molecular biology foundation; (4) understanding of several virulence phenotypes; (5) well-studied pathophysiology; and (6) robust animal models. With the use of a sequenced genome and site-directed mutagenesis to produce specific null mutants, the virulence composite of C. neoformans has begun to be identified one gene at a time. Studies into capsule production, melanin synthesis, high temperature growth, metabolic pathways and a variety of signaling pathways have led to understandings of what makes this yeast a pathogen at the molecular level. Multiple principles of molecular pathogenesis have been demonstrated in virulence studies with C. neoformans. These include evolutionary differences between the varieties of C. neoformans in their genes for virulence, quantitative impact of genes on the virulence composite, species and site-specific importance of a virulence gene, gene expression correlation with its functional importance or phenotype and the impact of a pathogenesis gene on the host immune response. C. neoformans has now become a primary model to study molecular fungal pathogenesis with the goal of identifying drug targets or vaccine strategies.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 3","pages":"Pages 395-404"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.06.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25222151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 102

Subject Index Volume 45 主题索引第45卷

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/S0928-8244(05)00183-5

引用次数: 0

Functional genome of the human pathogenic fungus Paracoccidioides brasiliensis 人类致病真菌巴西副球虫的功能基因组

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/j.femsim.2005.05.013

Maria Sueli S. Felipe , Fernando A.G. Torres , Andrea Q. Maranhão , Ildinete Silva-Pereira , Marcio J. Poças-Fonseca , Elida G. Campos , Lídia M.P. Moraes , Fabrício B.M. Arraes , Maria José A. Carvalho , Rosângela V. Andrade , André M. Nicola , Marcus M. Teixeira , Rosália S.A. Jesuíno , Maristela Pereira , Célia M.A. Soares , Marcelo M. Brígido

{"title":"Functional genome of the human pathogenic fungus Paracoccidioides brasiliensis","authors":"Maria Sueli S. Felipe , Fernando A.G. Torres , Andrea Q. Maranhão , Ildinete Silva-Pereira , Marcio J. Poças-Fonseca , Elida G. Campos , Lídia M.P. Moraes , Fabrício B.M. Arraes , Maria José A. Carvalho , Rosângela V. Andrade , André M. Nicola , Marcus M. Teixeira , Rosália S.A. Jesuíno , Maristela Pereira , Célia M.A. Soares , Marcelo M. Brígido","doi":"10.1016/j.femsim.2005.05.013","DOIUrl":"10.1016/j.femsim.2005.05.013","url":null,"abstract":"<div>Paracoccidioides brasiliensis is a dimorphic and thermo-regulated fungus which is the causative agent of paracoccidioidomycosis, an endemic disease widespread in Latin America. Pathogenicity is assumed to be a consequence of the cellular differentiation process that this fungus undergoes from mycelium to yeast cells during human infection. In an effort to elucidate the molecular mechanisms involved in this process a network of Brazilian laboratories carried out a transcriptome project for both cell types. This review focuses on the data analysis yielding a comprehensive view of the fungal metabolism and the molecular adaptations during dimorphism in P. brasiliensis from analysis of 6022 groups, related to expressed genes, which were generated from both mycelium and yeast phases.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 3","pages":"Pages 369-381"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25225742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Towards a molecular diagnosis of invasive aspergillosis and disseminated candidosis 侵袭性曲霉病和弥散性念珠菌病的分子诊断

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/j.femsim.2005.05.012

Stéphane Bretagne , Jean-Marc Costa

{"title":"Towards a molecular diagnosis of invasive aspergillosis and disseminated candidosis","authors":"Stéphane Bretagne , Jean-Marc Costa","doi":"10.1016/j.femsim.2005.05.012","DOIUrl":"10.1016/j.femsim.2005.05.012","url":null,"abstract":"<div>A lot of in-house polymerase chain reaction assays have been reported for diagnosis of invasive aspergillosis and disseminated candidosis. Encouraging results have been published to anticipate the diagnosis over the conventional microbiological methods. However, the absence of standardized methods has led to diverging results. As a consequence, these tests are not recognized as consensual diagnostic criteria, in contrast with some antigenemia detection kits. The major breakthrough for improving the results of these methods is the emergence of real-time technologies. This markedly improves the reliability of the PCR results by dramatically decreasing the risk of false positive results due to PCR products carryover. Moreover, using the quantitative results provided by this technique, this allows to rapidly compare the efficiency of primers, probes, and DNA extraction methods. Therefore, the hope is to identify the more specific and sensitive parameters to implement comparative studies. Automated DNA extraction should also be useful to achieve this goal.Whatever sophisticated technology is used, we still have to define the meaning of detecting nucleic acids in a given clinical sample. This seems simple in normally sterile anatomical sites but less obvious for example in respiratory specimens for invasive aspergillosis or in blood for candidosis in heavily colonized patients. Additional studies of the kinetics of fungal DNA are needed. The development of real-time technology should improve our knowledge in order to give the clinicians informative clues for making a decision.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 3","pages":"Pages 361-368"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25220846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 76

Biosynthesis of glycoproteins in the pathogenic fungus Candida albicans: Activation of dolichol phosphate mannose synthase by cAMP-mediated protein phosphorylation 白色念珠菌中糖蛋白的生物合成:camp介导的蛋白磷酸化激活磷酸甘露糖合成酶

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/j.femsim.2005.05.016

Blanca L. Arroyo-Flores, Carlos Calvo-Méndez, Arturo Flores-Carreón, Everardo López-Romero

{"title":"Biosynthesis of glycoproteins in the pathogenic fungus Candida albicans: Activation of dolichol phosphate mannose synthase by cAMP-mediated protein phosphorylation","authors":"Blanca L. Arroyo-Flores, Carlos Calvo-Méndez, Arturo Flores-Carreón, Everardo López-Romero","doi":"10.1016/j.femsim.2005.05.016","DOIUrl":"10.1016/j.femsim.2005.05.016","url":null,"abstract":"<div>Following incubation with ATP and a cAMP-dependent protein kinase under optimal conditions of lipid acceptor, phospholipid and metal ion requirements, the transfer activity of partially purified dolichol phosphate mannose synthase (DPMS) increased about 60% and this activation correlated with a 50% increase in Vmax with no alteration in the apparent Km for GDP-Manose. Phosphorylation with [γ-32P]ATP resulted in the labeling of several polypeptides, one of which exhibited the molecular weight of the enzyme (30 kDa) and was also recognized using a specific anti-DPMS monoclonal antibody. This and the fact that the phosphate label could be removed by an alkaline phosphatase indicate that Candida DPMS may be regulated by phosphorylation–dephosphorylation, a mechanism that has been proposed for the enzyme in other organisms.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 3","pages":"Pages 429-434"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25222150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Homology, disruption and phenotypic analysis of CaGS Candida albicans gene induced during macrophage infection 巨噬细胞感染诱导白色念珠菌基因cag的同源性、破坏及表型分析

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/j.femsim.2005.06.007

Marina Luongo , Amalia Porta , Bruno Maresca

引用次数: 11

Geographical distribution of genetic polymorphism of the pathogen Histoplasma capsulatum isolated from infected bats, captured in a central zone of Mexico 从墨西哥中部地区捕获的受感染蝙蝠中分离出的病原体荚膜组织浆体遗传多态性的地理分布

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/j.femsim.2005.05.019

Maria Lucia Taylor , Catalina B. Chávez-Tapia , Alberto Rojas-Martínez , Maria del Rocio Reyes-Montes , Mirian Bobadilla del Valle , Gerardo Zúñiga

{"title":"Geographical distribution of genetic polymorphism of the pathogen Histoplasma capsulatum isolated from infected bats, captured in a central zone of Mexico","authors":"Maria Lucia Taylor , Catalina B. Chávez-Tapia , Alberto Rojas-Martínez , Maria del Rocio Reyes-Montes , Mirian Bobadilla del Valle , Gerardo Zúñiga","doi":"10.1016/j.femsim.2005.05.019","DOIUrl":"10.1016/j.femsim.2005.05.019","url":null,"abstract":"<div>Fourteen Histoplasma capsulatum isolates recovered from infected bats captured in Mexican caves and two human H. capsulatum reference strains were analyzed using random amplification of polymorphic DNA PCR-based and partial DNA sequences of four genes. Cluster analysis of random amplification of polymorphic DNA-patterns revealed differences for two H. capsulatum isolates of one migratory bat Tadarida brasiliensis. Three groups were identified by distance and maximum-parsimony analyses of arf, H-anti, ole, and tub1 H. capsulatum genes. Group I included most isolates from infected bats and one clinical strain from central Mexico; group II included the two isolates from T. brasiliensis; the human G-217B reference strain from USA formed an independent group III. Isolates from group II showed diversity in relation to groups I and III, suggesting a different H. capsulatum population.</div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 3","pages":"Pages 451-458"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25225738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Author Index Volume 45 作者索引第45卷

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI: 10.1016/S0928-8244(05)00182-3

引用次数: 0