{"title":"巨噬细胞感染诱导白色念珠菌基因cag的同源性、破坏及表型分析","authors":"Marina Luongo , Amalia Porta , Bruno Maresca","doi":"10.1016/j.femsim.2005.06.007","DOIUrl":null,"url":null,"abstract":"<div><p>During macrophage infection <span><em>Candida albicans</em></span> expresses differentially several genes whose functions are associated with its survival strategy. Among others, we have isolated <em>CaGS</em> gene, which is homologous to <em>SNF3</em>, a glucose sensor of <span><em>Saccharomyces cerevisiae</em></span>. To elucidate its potential role during infection, <em>CaGS</em> has been disrupted and the resulting phenotype analyzed on different solid media. The null mutant lost the ability to form hyphae on a medium with low glucose concentration and serum. Furthermore, this mutant does not disrupt macrophage in in vitro infections. We believe that this putative glucose sensor is involved in hyphal development during macrophage infection.</p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 3","pages":"Pages 471-478"},"PeriodicalIF":0.0000,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.06.007","citationCount":"11","resultStr":"{\"title\":\"Homology, disruption and phenotypic analysis of CaGS Candida albicans gene induced during macrophage infection\",\"authors\":\"Marina Luongo , Amalia Porta , Bruno Maresca\",\"doi\":\"10.1016/j.femsim.2005.06.007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>During macrophage infection <span><em>Candida albicans</em></span> expresses differentially several genes whose functions are associated with its survival strategy. Among others, we have isolated <em>CaGS</em> gene, which is homologous to <em>SNF3</em>, a glucose sensor of <span><em>Saccharomyces cerevisiae</em></span>. To elucidate its potential role during infection, <em>CaGS</em> has been disrupted and the resulting phenotype analyzed on different solid media. The null mutant lost the ability to form hyphae on a medium with low glucose concentration and serum. Furthermore, this mutant does not disrupt macrophage in in vitro infections. We believe that this putative glucose sensor is involved in hyphal development during macrophage infection.</p></div>\",\"PeriodicalId\":12220,\"journal\":{\"name\":\"FEMS immunology and medical microbiology\",\"volume\":\"45 3\",\"pages\":\"Pages 471-478\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.femsim.2005.06.007\",\"citationCount\":\"11\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"FEMS immunology and medical microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0928824405001574\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEMS immunology and medical microbiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0928824405001574","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Homology, disruption and phenotypic analysis of CaGS Candida albicans gene induced during macrophage infection
During macrophage infection Candida albicans expresses differentially several genes whose functions are associated with its survival strategy. Among others, we have isolated CaGS gene, which is homologous to SNF3, a glucose sensor of Saccharomyces cerevisiae. To elucidate its potential role during infection, CaGS has been disrupted and the resulting phenotype analyzed on different solid media. The null mutant lost the ability to form hyphae on a medium with low glucose concentration and serum. Furthermore, this mutant does not disrupt macrophage in in vitro infections. We believe that this putative glucose sensor is involved in hyphal development during macrophage infection.