Molecular characteristics of community-acquired, methicillin-resistant Staphylococcus aureus isolated from Chinese children.

Wenjing Geng, Yonghong Yang, Dejing Wu, Guoying Huang, Chuanqing Wang, Li Deng, Yuejie Zheng, Zhou Fu, Changcong Li, Yunxiao Shang, Changan Zhao, Sangjie Yu, Xuzhuang Shen
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引用次数: 56

Abstract

The aim of this study was to investigate the molecular characteristics of community-acquired, methicillin-resistant Staphylococcus aureus (CA-MRSA) isolates from Chinese children. Ninety-nine isolates were collected from eight hospitals, and analyzed by multilocus sequence typing, staphylococcal chromosomal cassette mec (SCCmec) type, and spa typing. The Panton-Valentine leukocidin (PVL) gene was also detected. Overall, 14 sequence types (STs) were obtained, and ST59 (58.6%) was found to be the most prevalent, followed by ST1 (8%) and ST338 (8%). We also first registered the new ST1409. SCCmec type IV was the most predominant type at 67.7%, followed by SCCmec type V at 32.3%. SCCmec subtypes IVa, IVc, and IVg were found among the SCCmec type IV strains. Twenty-one spa types were also identified. Four new spa types were found by synchronization with the Ridom SpaServer and referring to the website (http://www.SeqNet.org). ST59-MRSA-IVa with t437 accounted for 40.4% of occurrences, making it the most prevalent clone. The prevalence of PVL genes was 58.6%, and multidrug resistance was observed in 95% of all isolates. This result indicates that CA-MRSA isolates in Chinese children are largely associated with the ST59-MRSA-IV clone, and that the predominant clones of CA-MRSA are spread all over the country.

中国儿童社区获得性耐甲氧西林金黄色葡萄球菌的分子特征。
本研究旨在探讨中国儿童社区获得性耐甲氧西林金黄色葡萄球菌(CA-MRSA)分离株的分子特征。从8家医院收集99株葡萄球菌,采用多位点序列分型、葡萄球菌染色体盒式mec (SCCmec)分型和spa分型进行分析。Panton-Valentine leukocidin (PVL)基因也被检测到。总共获得14种序列类型(STs),其中ST59(58.6%)最为常见,其次是ST1(8%)和ST338(8%)。我们也首先注册了新的ST1409。SCCmec IV型占67.7%,其次是SCCmec V型占32.3%。SCCmec IV型株中存在IVa、IVc和IVg亚型。还确定了21种spa类型。通过与Ridom SpaServer同步并参考网站(http://www.SeqNet.org),发现了四种新的spa类型。携带t437的ST59-MRSA-IVa占40.4%,是最常见的克隆。PVL基因阳性率为58.6%,耐多药率为95%。这一结果表明,中国儿童CA-MRSA分离株主要与ST59-MRSA-IV克隆相关,CA-MRSA优势克隆分布在全国各地。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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