Fly最新文献_第8页

Epithelial tumors: Growing from within. 上皮性肿瘤:从内部生长。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-01 Epub Date: 2018-03-01 DOI: 10.1080/19336934.2018.1441652

Mariana Muzzopappa, Marco Milán

{"title":"Epithelial tumors: Growing from within.","authors":"Mariana Muzzopappa, Marco Milán","doi":"10.1080/19336934.2018.1441652","DOIUrl":"https://doi.org/10.1080/19336934.2018.1441652","url":null,"abstract":"The growth of epithelial tumors is often governed by cell interactions with the surrounding stroma. Drosophila has been instrumental in identifying the relevant molecular elements mediating these interactions. Of note is the role of the TNF ligand Eiger, released from recruited blood cells, in activating the JNK tumor-promoting pathway in epithelial tumors. JNK drives the transcriptional induction of mitogenic molecules, matrix metalloproteases and systemic signals that lead to tumor growth, tissue invasiveness and malignancy. Here we review our findings on a tumor-intrinsic, Eiger- and stroma-independent mechanism that contributes to the unlimited growth potential of tumors caused either by chromosomal instability or impaired cell polarity. This newly identified mechanism, which was revealed in an experimental condition in which contacts between tumor cells and wild-type epithelial cells were minimized, relies on interactions between functionally distinct tumor cell populations that activate JNK in a cell-autonomous manner. We discuss the impact of cell interaction-based feedback amplification loops on the unlimited growth potential of epithelial tumors. These findings are expected to contribute to the identification of the relevant cell populations and molecular mechanisms to be targeted in drug therapy.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2018.1441652","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35837647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary management and physical exercise can improve climbing defects and mitochondrial activity in Drosophila melanogaster parkin null mutants. 饮食管理和体育锻炼可以改善黑腹果蝇parkin零突变体的攀爬缺陷和线粒体活性。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-01 Epub Date: 2018-08-01 DOI: 10.1080/19336934.2018.1482139

Rijan Bajracharya, J William O Ballard

{"title":"Dietary management and physical exercise can improve climbing defects and mitochondrial activity in Drosophila melanogaster parkin null mutants.","authors":"Rijan Bajracharya, J William O Ballard","doi":"10.1080/19336934.2018.1482139","DOIUrl":"https://doi.org/10.1080/19336934.2018.1482139","url":null,"abstract":"Physical exercise can improve gait, balance, tremor, flexibility, grip strength and motor coordination in Parkinson's disease (PD) patients. Several lines of evidence have also shown the therapeutic potential of dietary management and supplementation in halting the progression of PD. However, there is a lack of research on the combined effects of physical activity and nutrition in the progression of PD. We test the effects exercise and dietary modification in a Drosophila model of PD. In this study, we fed Drosophila parkin mutants high protein and high carbohydrate diets without and with stearic acid (4 treatments in total). In parallel, we subjected mutants to a regimen of exercise using a purpose-built 'Power tower' exercise machine. We then measured climbing ability, aconitase activity, and basal mitochondrial ROS levels. We observed that exercising parkin mutants fed the high protein diet improved their climbing ability and increased aconitase activity. There was an additional improvement in climbing and aconitase activity in exercised parkin mutants fed the high protein diet supplemented with stearic acid. No benefits of exercise were seen in parkin mutants fed the high carbohydrate diet. Combined, these results suggest that dietary management along with physical activty has potential to improve mitochondrial biogenesis and delay the progression of PD in Drosophila parkin mutants.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2018.1482139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36363210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Maintenance of Rhodopsin levels in Drosophila photoreceptor and phototransduction requires Protein Kinase D. 果蝇光感受器和光传导中视紫红质水平的维持需要蛋白激酶D。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-01 Epub Date: 2019-02-05 DOI: 10.1080/19336934.2019.1565256

Sudipta Ashe, Shweta Yadav

引用次数: 1

A model-based high throughput method for fecundity estimation in fruit fly studies. 基于模型的果蝇繁殖力估计高通量方法。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-01 Epub Date: 2018-12-30 DOI: 10.1080/19336934.2018.1562267

Enoch Ng'oma, Elizabeth G King, Kevin M Middleton

引用次数: 5

Drosophila strain specific response to cisplatin neurotoxicity. 果蝇对顺铂神经毒性的特异性反应。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-01 Epub Date: 2019-01-22 DOI: 10.1080/19336934.2019.1565257

Christopher M Groen, Jewel L Podratz, Kevin Treb, Anthony J Windebank

引用次数: 9

The COP9 signalosome inhibits Cullin-RING E3 ubiquitin ligases independently of its deneddylase activity. COP9信号体对Cullin-RING E3泛素连接酶的抑制独立于其去醛化酶活性。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-01 Epub Date: 2018-02-09 DOI: 10.1080/19336934.2018.1429858

Annabelle Suisse, Miklós Békés, Tony T Huang, Jessica E Treisman

{"title":"The COP9 signalosome inhibits Cullin-RING E3 ubiquitin ligases independently of its deneddylase activity.","authors":"Annabelle Suisse, Miklós Békés, Tony T Huang, Jessica E Treisman","doi":"10.1080/19336934.2018.1429858","DOIUrl":"https://doi.org/10.1080/19336934.2018.1429858","url":null,"abstract":"The COP9 signalosome inhibits the activity of Cullin-RING E3 ubiquitin ligases by removing Nedd8 modifications from their Cullin subunits. Neddylation renders these complexes catalytically active, but deneddylation is also necessary for them to exchange adaptor subunits and avoid auto-ubiquitination. Although deneddylation is thought to be the primary function of the COP9 signalosome, additional activities have been ascribed to some of its subunits. We recently showed that COP9 subunits protect the transcriptional repressor and tumor suppressor Capicua from two distinct modes of degradation. Deneddylation by the COP9 signalosome inactivates a Cullin 1 complex that ubiquitinates Capicua following its phosphorylation by MAP kinase in response to Epidermal Growth Factor Receptor signaling. The CSN1b subunit also stabilizes unphosphorylated Capicua to control its basal level, independently of the deneddylase function of the complex. Here we further examine the importance of deneddylation for COP9 functions in vivo. We use an uncleavable form of Nedd8 to show that preventing deneddylation does not reproduce the effects of loss of COP9. In contrast, in the presence of COP9, conjugation to uncleavable Nedd8 renders Cullins unable to promote the degradation of their substrates. Our results suggest that irreversible neddylation prolongs COP9 binding to and inhibition of Cullin-based ubiquitin ligases.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2018.1429858","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35755701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Sex-lethal in neurons controls female body growth in Drosophila. 性致死性神经元控制果蝇雌性身体的生长。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-01 Epub Date: 2018-08-21 DOI: 10.1080/19336934.2018.1502535

Annick Sawala, Alex P Gould

{"title":"Sex-lethal in neurons controls female body growth in Drosophila.","authors":"Annick Sawala, Alex P Gould","doi":"10.1080/19336934.2018.1502535","DOIUrl":"https://doi.org/10.1080/19336934.2018.1502535","url":null,"abstract":"Sexual size dimorphism (SSD), a sex difference in body size, is widespread throughout the animal kingdom, raising the question of how sex influences existing growth regulatory pathways to bring about SSD. In insects, somatic sexual differentiation has long been considered to be controlled strictly cell-autonomously. Here, we discuss our surprising finding that in Drosophila larvae, the sex determination gene Sex-lethal (Sxl) functions in neurons to non-autonomously specify SSD. We found that Sxl is required in specific neuronal subsets to upregulate female body growth, including in the neurosecretory insulin producing cells, even though insulin-like peptides themselves appear not to be involved. SSD regulation by neuronal Sxl is also independent of its known splicing targets, transformer and msl-2, suggesting that it involves a new molecular mechanism. Interestingly, SSD control by neuronal Sxl is selective for larval, not imaginal tissue types, and operates in addition to cell-autonomous effects of Sxl and Tra, which are present in both larval and imaginal tissues. Overall, our findings add to a small but growing number of studies reporting non-autonomous, likely hormonal, control of sex differences in Drosophila, and suggest that the principles of sexual differentiation in insects and mammals may be more similar than previously thought.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2018.1502535","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36411248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Generation of genome-modified Drosophila cell lines using SwAP. 利用SwAP产生基因组修饰的果蝇细胞系。

IF 1.2 4区生物学

Fly Pub Date : 2017-10-02 Epub Date: 2017-08-30 DOI: 10.1080/19336934.2017.1372068

Alexandra Franz, Erich Brunner, Konrad Basler

引用次数: 5

Dual role of BMP signaling in the regulation of Drosophila intestinal stem cell self-renewal. BMP信号在果蝇肠道干细胞自我更新调控中的双重作用。

IF 1.2 4区生物学

Fly Pub Date : 2017-10-02 Epub Date: 2017-09-25 DOI: 10.1080/19336934.2017.1384104

Aiguo Tian, Jin Jiang

{"title":"Dual role of BMP signaling in the regulation of Drosophila intestinal stem cell self-renewal.","authors":"Aiguo Tian, Jin Jiang","doi":"10.1080/19336934.2017.1384104","DOIUrl":"https://doi.org/10.1080/19336934.2017.1384104","url":null,"abstract":"Many adult organs including Drosophila adult midguts rely on resident stem cells to replenish damaged cells during tissue homeostasis and regeneration. Previous studies have shown that, upon injury, intestinal stem cells (ISCs) in the midguts can increase proliferation and lineage differentiation to meet the demand for tissue repair. Our recent study has demonstrated that, in response to certain injury, midguts can expand ISC population size as an additional regenerative mechanism. We found that injury elicited by bleomycin feeding or bacterial infection increased the production of two BMP ligands (Dpp and Gbb) in enterocytes (ECs), leading to elevated BMP signaling in progenitor cells that drove an expansion of ISCs by promoting their symmetric self-renewing division. Interestingly, we also found that BMP signaling in ECs inhibits the production of Dpp and Gbb, and that this negative feedback mechanism is required to reset ISC pool size to the homeostatic state. Our findings suggest that BMP signaling exerts two opposing influences on stem cell activity depending on where it acts: BMP signaling in progenitor cells promotes ISC self-renewal while BMP signaling in ECs restricts ISC self-renewal by preventing excessive production of BMP ligands. Our results further suggest that transient expansion of ISC population in conjunction with increasing ISC proliferation provides a more effective strategy for tissue regeneration.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2017-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2017.1384104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35441060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Comparative analysis of behavioral and transcriptional variation underlying CO₂ sensory neuron function and development in Drosophila. 果蝇二氧化碳感觉神经元功能和发育的行为和转录变异的比较分析。

IF 1.2 4区生物学

Fly Pub Date : 2017-10-02 Epub Date: 2017-06-23 DOI: 10.1080/19336934.2017.1344374

Jia Wern Pan, Joi McLaughlin, Haining Yang, Charles Leo, Paula Rambarat, Sumie Okuwa, Anaïs Monroy-Eklund, Sabrina Clark, Corbin D Jones, Pelin Cayirlioglu Volkan

{"title":"Comparative analysis of behavioral and transcriptional variation underlying CO2 sensory neuron function and development in Drosophila.","authors":"Jia Wern Pan, Joi McLaughlin, Haining Yang, Charles Leo, Paula Rambarat, Sumie Okuwa, Anaïs Monroy-Eklund, Sabrina Clark, Corbin D Jones, Pelin Cayirlioglu Volkan","doi":"10.1080/19336934.2017.1344374","DOIUrl":"https://doi.org/10.1080/19336934.2017.1344374","url":null,"abstract":"Carbon dioxide is an important environmental cue for many insects, regulating many behaviors including some that have direct human impacts. To further improve our understanding of how this system varies among closely related insect species, we examined both the behavioral response to CO2 as well as the transcriptional profile of key developmental regulators of CO2 sensory neurons in the olfactory system across the Drosophila genus. We found that CO2 generally evokes repulsive behavior across most of the Drosophilids we examined, but this behavior has been lost or reduced in several lineages. Comparisons of transcriptional profiles from the developing and adult antennae for subset these species suggest that behavioral differences in some species may be due to differences in the expression of the CO2 co-receptor Gr63a. Furthermore, these differences in Gr63a expression are correlated with changes in the expression of a few genes known to be involved in the development of the CO2 circuit, namely dac, an important regulator of sensilla fate for sensilla that house CO2 ORNs, and mip120, a member of the MMB/dREAM epigenetic regulatory complex that regulates CO2 receptor expression. In contrast, most of the other known structural, molecular, and developmental components of the peripheral Drosophila CO2 olfactory system seem to be well-conserved across all examined lineages. These findings suggest that certain components of CO2 sensory ORN development may be more evolutionarily labile, and may contribute to differences in CO2-evoked behavioral responses across species.","PeriodicalId":12128,"journal":{"name":"Fly","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2017-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2017.1344374","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35114722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4