Strong responses of Drosophila melanogaster microbiota to developmental temperature.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Fly Pub Date : 2018-01-02 Epub Date: 2017-12-07 DOI:10.1080/19336934.2017.1394558

Neda N Moghadam, Pia Mai Thorshauge, Torsten N Kristensen, Nadieh de Jonge, Simon Bahrndorff, Henrik Kjeldal, Jeppe Lund Nielsen

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引用次数: 76

Abstract

Physiological responses to changes in environmental conditions such as temperature may partly arise from the resident microbial community that integrates a wide range of bio-physiological aspects of the host. In the present study, we assessed the effect of developmental temperature on the thermal tolerance and microbial community of Drosophila melanogaster. We also developed a bacterial transplantation protocol in order to examine the possibility of reshaping the host bacterial composition and assessed its influence on the thermotolerance phenotype. We found that the temperature during development affected thermal tolerance and the microbial composition of male D. melanogaster. Flies that developed at low temperature (13°C) were the most cold resistant and showed the highest abundance of Wolbachia, while flies that developed at high temperature (31°C) were the most heat tolerant and had the highest abundance of Acetobacter. In addition, feeding newly eclosed flies with bacterial suspensions from intestines of flies developed at low temperatures changed the heat tolerance of recipient flies. However, we were not able to link this directly to a change in the host bacterial composition.

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黑腹果蝇微生物群对发育温度的强烈反应。

对环境条件(如温度)变化的生理反应可能部分来自宿主的常驻微生物群落，它们整合了宿主广泛的生物生理方面。在本研究中，我们评估了发育温度对黑腹果蝇耐热性和微生物群落的影响。我们还开发了一种细菌移植方案，以检查重塑宿主细菌组成的可能性，并评估其对耐热表型的影响。研究发现，发育过程中的温度影响雄性黑腹田鼠的耐热性和微生物组成。在低温(13℃)条件下发育的蝇类抗寒性最强，沃尔巴克氏体菌丰度最高，而在高温(31℃)条件下发育的蝇类耐热性最强，醋酸杆菌丰度最高。此外，用低温下发育的果蝇肠道的细菌悬浮液喂养新封闭的果蝇会改变受体果蝇的耐热性。然而，我们无法将这与宿主细菌组成的变化直接联系起来。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fly 生物-生化与分子生物学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Fly is the first international peer-reviewed journal to focus on Drosophila research. Fly covers a broad range of biological sub-disciplines, ranging from developmental biology and organogenesis to sensory neurobiology, circadian rhythm and learning and memory, to sex determination, evolutionary biology and speciation. We strive to become the “to go” resource for every researcher working with Drosophila by providing a forum where the specific interests of the Drosophila community can be discussed. With the advance of molecular technologies that enable researchers to manipulate genes and their functions in many other organisms, Fly is now also publishing papers that use other insect model systems used to investigate important biological questions. Fly offers a variety of papers, including Original Research Articles, Methods and Technical Advances, Brief Communications, Reviews and Meeting Reports. In addition, Fly also features two unconventional types of contributions, Counterpoints and Extra View articles. Counterpoints are opinion pieces that critically discuss controversial papers questioning current paradigms, whether justified or not. Extra View articles, which generally are solicited by Fly editors, provide authors of important forthcoming papers published elsewhere an opportunity to expand on their original findings and discuss the broader impact of their discovery. Extra View authors are strongly encouraged to complement their published observations with additional data not included in the original paper or acquired subsequently.