Localization and translation control of slam in Drosophila cellularization.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Fly Pub Date : 2018-01-01 Epub Date: 2018-09-18 DOI:10.1080/19336934.2018.1520574

Shuling Yan, Jörg Großhans

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引用次数: 4

Abstract

In this extra view, we comment on our recent work concerning the mRNA localization of the gene slow as molasses (slam). slam is a gene essential for the polarized invagination of the plasma membrane and separation of basal and lateral cortical domains during cellularization as well as for germ cell migration in later embryogenesis. We have demonstrated an intimate relationship between slam RNA and its encoded protein. Slam RNA co-localizes and forms a complex with its encoded protein. Slam mRNA localization not only is required for reaching full levels of functional Slam protein but also depends on Slam protein. The translation of slam mRNA is subject to tight spatio-temporal regulation leading to a rapid accumulation of Slam protein and zygotic slam RNA at the furrow canal. In this extra view, we first discuss the mechanism controlling localization and translation of slam RNA. In addition, we document in detail the maternal and zygotic expression of slam RNA and protein and provide data for a function in membrane stabilization. Furthermore, we mapped the region of Slam protein mediating cortical localization in cultured cells.

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果蝇细胞化过程中slam的定位与翻译控制。

在这个额外的观点中，我们评论了我们最近关于基因的mRNA定位慢如糖蜜(slam)的工作。Slam是细胞化过程中质膜极化内陷、基底和外侧皮质区分离以及胚胎发生后期生殖细胞迁移所必需的基因。我们已经证明了slam RNA与其编码蛋白之间的密切关系。Slam RNA与其编码蛋白共定位并形成复合体。Slam mRNA的定位不仅是达到功能完备的Slam蛋白水平所必需的，而且依赖于Slam蛋白。slam mRNA的翻译受严格的时空调控，导致slam蛋白和合子slam RNA在沟管处快速积累。在这个额外的观点中，我们首先讨论了控制slam RNA定位和翻译的机制。此外，我们详细记录了slam RNA和蛋白在母体和合子中的表达，并为其在膜稳定中的功能提供了数据。此外，我们绘制了Slam蛋白在培养细胞中介导皮质定位的区域。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fly 生物-生化与分子生物学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Fly is the first international peer-reviewed journal to focus on Drosophila research. Fly covers a broad range of biological sub-disciplines, ranging from developmental biology and organogenesis to sensory neurobiology, circadian rhythm and learning and memory, to sex determination, evolutionary biology and speciation. We strive to become the “to go” resource for every researcher working with Drosophila by providing a forum where the specific interests of the Drosophila community can be discussed. With the advance of molecular technologies that enable researchers to manipulate genes and their functions in many other organisms, Fly is now also publishing papers that use other insect model systems used to investigate important biological questions. Fly offers a variety of papers, including Original Research Articles, Methods and Technical Advances, Brief Communications, Reviews and Meeting Reports. In addition, Fly also features two unconventional types of contributions, Counterpoints and Extra View articles. Counterpoints are opinion pieces that critically discuss controversial papers questioning current paradigms, whether justified or not. Extra View articles, which generally are solicited by Fly editors, provide authors of important forthcoming papers published elsewhere an opportunity to expand on their original findings and discuss the broader impact of their discovery. Extra View authors are strongly encouraged to complement their published observations with additional data not included in the original paper or acquired subsequently.