Experimental and Applied Acarology最新文献

{"title":"The first complete mitochondrial genome of the genus Laelaps with novel gene arrangement reveals extensive rearrangement and phylogenetics in the superfamily Dermanyssoidea.","authors":"Bili Yuan, Gangxian He, Wenge Dong","doi":"10.1007/s10493-024-00943-2","DOIUrl":"10.1007/s10493-024-00943-2","url":null,"abstract":"<p><p>We collected 56 specimens of Laelaps chini from the endemic Hengduan Mountain rat species (Eothenomys miletus) and obtained the first complete mitochondrial genome of L. chini by next-generation sequencing (NGS). The L. chini mitogenome is 16,507 bp in size and contains 37 genes and a control region of 2380 bp in length. The L. chini mitogenome has a high AT content and a compact arrangement with four overlapping regions ranging from 1 to 2 bp and 16 spacer regions ranging from 1 to 48 bp. We analyzed 13 protein-coding genes of L. chini mitogenome  and found that protein-coding genes in the L. chini mitogenome preferred codons ending in A/U and codon usage pattern was mainly influenced by natural selection. Cox1 has the slowest evolution rate and cox3 has the fastest evolution rate. We combined the mitochondrial genome of eight species of gamasid mites in the superfamily Dermanyssoidea from Genbank and the L. chini mitochondrial genome to analyze its rearrangement patterns and breakpoint numbers. We found that the L. chini mitogenome showed a novel arrangement pattern and nine species of gamasid mites in the superfamily Dermanyssoidea, which have been sequenced complete mitochondrial genomes to date, all showed different degrees of rearrangement. Laelaps chini, Echinolaelaps echidninus and Echinolaelaps fukinenensis were closely related species based on genetic distance and phylogenetic analyses. Notably they are clustered with Varroa destructor of the family Varroidae, suggesting that the family Varroidae is more closely related to the family Laelapidae, but more data are needed to test whether Varroa can be classified under the family Laelapidae. The L. chini mitogenome is the first complete mitochondrial genome for the genus Laelaps, and contributes to further exploration of the mitochondrial gene rearrangements and phylogeny for the superfamily Dermanyssoidea.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":" ","pages":"515-535"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ambulatory dispersal of Typhlodromus (Anthoseius) recki Wainstein (Acari: Phytoseiidae) along Solanceae stem.","authors":"M-S Tixier, A Raeckelboom, L Tabary, M Douin, M Navajas, D Navia","doi":"10.1007/s10493-024-00946-z","DOIUrl":"10.1007/s10493-024-00946-z","url":null,"abstract":"<p><p>Tomato crops are attacked by several pests, including mites. While the main predatory mites are not effective enough to control mite pests, recent studies have shown encouraging results with the European endemic phytoseiid Typhlodromus (Anthoseius) recki. The first objective of the study was to assess the ability of this species to disperse along the tomato stem, considering six genotypes of Solanum lycopersicum, S. peruvianum and S. cheesmaniae with contrasted trichome numbers and types of stem trichomes, accuratetly characterised in a previous study. The second objective was to determine how predator morphological traits can explain dispersal along the tomato stem. For this, ambulatory dispersal ability of females (stem crossing rate success, hesitation and escape behavior, mobility periods) was tested in lab conditions on the eight Solanum genotypes, at four period of time after the predator introduction (10, 25, 55 and 100 min), with a video observation of 5 min at each period. The females were then mounted on slides and body length and width (at the fore hind, middle and back parts) measured. No effect of the tomato genotypes was observed on the dispersal ability of the predator. However, specimens that succeeded in crossing the stem, had a higher percentage of mobility time (79.36%) than those that failed (43.60%). Furthermore, body width at midbody (DSW2) and dorsal shield length (DSL) were negatively correlated with dispersal ability. The mean DSL and DSW2 of the females that succeed to cross were 342.3 and 160.9 μm, respectively vs. 345.6 and 164.9 μm, for females that did not succeed. This suggests that the more slender and relatively small the specimens, the more are mobile and able to successfully cross the stem. The number of glandular trichomes type (GT) VI and to a lesser extent GT I and IV, and non-glandular trichomes (NGT) II&III appear to limit dispersal. The GT VI seems to have a repellent effect. On the opposite, the number of NGT V were positively correlated with high mobility and stem crossing rates. Assuming that the main barrier to biological control efficiency is dispersal along tomato stems, these preliminary results should have implications for biological control success. The proportion of mites with 'optimal dimensions' appears to be low and further studies should be undertaken to better assess the proportion of mites with such ideal dimensions in different populations and also to determine whether these morphological traits are associated with different feeding abilities and/or abiotic conditions.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":" ","pages":"563-582"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of different photoperiod regimes on vital life traits of Amblyseius swirskii and Neoseiulus cucumeris (Acari: Phytoseiidae).","authors":"Shima Yazdanpanah, Yaghoub Fathipour","doi":"10.1007/s10493-024-00949-w","DOIUrl":"10.1007/s10493-024-00949-w","url":null,"abstract":"<p><p>Environmental factors, such as photoperiod can play an important role in the development and performance of predatory mites. The influence of five photoperiod regimes (0: 24, 8: 16, 12: 12, 16: 8, and 24: 0 h; Light: Dark) on the population parameters of the predatory mites, Amblyseius swirskii Athias-Henriot and Neoseiulus cucumeris (Oudemans) was studied under the laboratory conditions at 25 °C and 25 ± 1 °C, 65 ± 5% RH. Regarding A. swirskii, the longest and shortest total pre-adult and total preoviposition periods (TPOP) were in the photoperiod of 12 L:12D and 16 L:8D, respectively. The oviposition days, adult longevity, total life span, fecundity, gross reproductive rate (GRR), and net reproductive rate (R₀) of the predator had no significant difference in all photoperiods tested. The intrinsic rate of increase (r) and the finite rate of increase (λ) in 16 L:8D photoperiod had no significant difference with the other photoperiods tested except 12 L:12D. Regarding N. cucumeris, the shortest total pre-adult and TPOP were in full darkness. The oviposition days and fecundity were significantly higher in 16 L:8D than the others. The value of R₀ in 16 L:8D was significantly higher than 8 L:16D. The highest values of the parameters r and λ were observed in full darkness, which had no significant difference with 16 L:8D and 24 L:0D. According to the results, the photoperiod regime had a more significant influence in terms of development and reproduction on N. cucumeris than A. swirskii, and 16:8 (L: D) h photoperiod was recognized as the proper regime for their rearing.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":" ","pages":"597-608"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sublethal effects of a spiromesifen and abamectin combination on Tetranychus urticae (Acari: Tetranychidae) and its predators Phytoseiulus persimilis and Amblyseius swirskii (Acari: Phytoseiiidae).","authors":"Elham Rezaei, Shahram Aramideh, J P Michaud, Shahram Mirfakhraie, Maryam Forouzan","doi":"10.1007/s10493-024-00941-4","DOIUrl":"10.1007/s10493-024-00941-4","url":null,"abstract":"<p><p>The two-spotted spider mite, Tetranychus urticae Koch (TSSM), is an important cosmopolitan pest of agricultural crops that is often managed in greenhouses by augmentation of predatory mites in combination with acaricides. Here we examined the transgenerational effects of low lethal concentrations of a widely-used acaricide, Oberon Speed® (a combination of spiromesifen and abamectin), on the life history traits and population growth of T. urticae and two of its predators, Phytoseiulus persimilis Athias-Henriot and Amblyseius swirskii Athias-Henriot (Acari: Phytoseiidae). The concentrations employed corresponded to the LC₁₀, LC₂₀ and LC₃₀ values estimated for TSSM protonymphs 48 h post-exposure in a topical bioassay, which yielded an LC₅₀ value of 207.2 ppm. Parental exposure of TSSM to all three low concentrations increased the total developmental time of progeny; both the LC₂₀ and LC₃₀ treatments reduced adult longevity and number of oviposition days, but only the LC₃₀ treatment increased the preoviposition period. Similarly, both the LC₂₀ and LC₃₀ treatments significantly reduced life table parameters (r, R₀, λ, and GRR), and increased generation time (T) and population doubling time (DT). Although maternal exposure to the acaricide had various impacts on progeny life history, A. swirskii was less affected than P. persimilis, suggesting the former species would be more compatible for integration with Oberon Speed® for control of T. urticae in greenhouse vegetable production.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":" ","pages":"501-514"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cladistics of Rhipicephalus microplus and laboratory assessment of acaricidal efficacy of different Cassia fistula extracts against R. microplus larvae.","authors":"Aman D Moudgil, Dinesh Sharma, Anil K Nehra, Damanpreet Singh, Prashant S Daundkar","doi":"10.1007/s10493-024-00947-y","DOIUrl":"10.1007/s10493-024-00947-y","url":null,"abstract":"<p><p>The present study aimed to analyze the cladistics and population structure analysis of Rhipicephalus microplus ticks infesting buffaloes in Haryana, India, as well as the assessment of the anti-tick efficacy of the ethanolic extracts of Cassia fistula (bark, pod pulp, and flowers) against R. microplus larvae. The molecular characterization and population structure analysis were performed by targeting the amplification of the partial mitochondrial cytochrome C oxidase subunit 1 (cox1) gene, whereas anti-tick efficacy was evaluated using a larval packet test. The sequences generated herein revealed a homology of 98.26-100% to the GenBank-archived R. microplus sequences. In population structure analysis, high haplotype (0.500 ± 0.265) and low nucleotide (0.002 ± 0.001) diversities were recorded for the sequences generated in this study. Significantly negative neutrality indices were recorded for the overall dataset. The extracts were found to significantly affect mortality rates in a dose-dependent manner, and the ethanolic extracts of the bark, pod pulp, and flowers of C. fistula exhibited median lethal concentration (LC₅₀) values of 27.989, 40.457, and 49.43 mg/mL, respectively. The LC₅₀ value recorded for the combination of the ethanolic extracts of the bark, flower, and pod pulp of C. fistula was 19.724 mg/mL, whereas the synthetic acaricide ivermectin had an LC₅₀ value of 351.56 mg/mL. In conclusion, R. microplus populations infesting cattle and buffalo hosts in India exhibited negligible genetic differentiation and high gene flow between them. Additionally, the combination of all C. fistula extracts could serve as a potential substitute for the synthetic acaricide.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":" ","pages":"645-663"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141563135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-based hyperspectral wavelength selection and classification of spider mite-infested cucumber leaves.","authors":"Boris Mandrapa, Klaus Spohrer, Dominik Wuttke, Ute Ruttensperger, Christine Dieckhoff, Joachim Müller","doi":"10.1007/s10493-024-00953-0","DOIUrl":"10.1007/s10493-024-00953-0","url":null,"abstract":"<p><p>Two-spotted spider mite (Tetranychus urticae) is an important greenhouse pest. In cucumbers, heavy infestations lead to the complete loss of leaf assimilation surface, resulting in plant death. Symptoms caused by spider mite feeding alter the light reflection of leaves and could therefore be optically detected. Machine learning methods have already been employed to analyze spectral information in order to differentiate between healthy and spider mite-infested leaves of crops such as tomatoes or cotton. In this study, machine learning methods were applied to cucumbers. Hyperspectral data of leaves were recorded under controlled conditions. Effective wavelengths were identified using three feature selection methods. Subsequently, three supervised machine learning algorithms were used to classify healthy and spider mite-infested leaves. All combinations of feature selection and classification methods yielded accuracy of over 80%, even when using ten or five wavelengths. These results suggest that machine learning methods are a powerful tool for image-based detection of spider mites in cucumbers. In addition, due to the limited number of wavelengths, there is also substantial potential for practical application.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":" ","pages":"627-644"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11464534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogens detected in ticks (Ixodes ricinus) feeding on red squirrels (Sciurus vulgaris) from city parks in Warsaw.","authors":"Dorota Dwużnik-Szarek, Agata Beliniak, Wiktoria Malaszewicz, Dagny Krauze-Gryz, Jakub Gryz, Karolina D Jasińska, Dagmara Wężyk, Anna Bajer","doi":"10.1007/s10493-024-00955-y","DOIUrl":"10.1007/s10493-024-00955-y","url":null,"abstract":"<p><p>The European red squirrel (Sciurus vulgaris) is a common host for Ixodes ricinus ticks in urban and rural habitats, however, studies on ticks and tick-borne pathogens (TBPs) of squirrels have not been conducted in Poland yet. Thus, the aims of the current study were to assess and compare the prevalence and abundance of ticks on red squirrels trapped at two sites in the Warsaw area (in an urban forest reserve and an urban park) and using molecular tools, to assess the genetic diversity of three pathogens (Borrelia burgdorferi sensu lato, Rickettsia and Babesia spp.) in I. ricinus ticks collected from squirrels. For the detection of Rickettsia spp. a 750 bp long fragment of the citrate synthase gltA gene was amplified; for B. burgdorferi s.l. 132f/905r and 220f/824r primers were used to amplify the bacterial flaB gene fragments (774 and 605 bp, respectively) and for Babesia spp., a 550 bpfragment of 18S rRNA gene was amplified. In total, 91 red squirrels were examined for ticks. There were differences in tick prevalence and mean abundance of infestation in squirrels from the urban forest reserve and urban park. Three species of B. burgdorferi s.l., Rickettsia spp., and Babesia microti were detected in ticks removed from the squirrels. Our results broaden knowledge of S. vulgaris as an important host for immature I. ricinus stages and support the hypothesis that red squirrels act as a reservoir of B. burgdorferi. Moreover, we conclude that red squirrels may also play a role in facilitating the circulation of other pathogens causing serious risk of tick-borne diseases in natural and urban areas.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":" ","pages":"677-699"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11464548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic rate does not scale with body size or activity in some tick species","authors":"Kayla N. Earls, Kennan J. Oyen","doi":"10.1007/s10493-024-00958-9","DOIUrl":"https://doi.org/10.1007/s10493-024-00958-9","url":null,"abstract":"<p>Respiration in ticks is highly efficient and exceptionally low. Ticks can survive years between bloodmeals by having low activity and respiration to conserve energetic resources. Our objective was to compare metabolic (VCO₂) and activity rates across 6 tick species. We predicted that VCO₂ would be different among species and scale linearly with activity and body mass. Activity and CO₂ production were measured for 32 h in 6 tick species: <i>Dermacentor andersoni</i>, <i>D. variabilis</i>, <i>Haemaphysalis longicornis</i>, <i>Rhipicephalus appendiculatus</i>, <i>R. microplus</i>, and <i>R. sanguineus</i>. Individual ticks were measured for 30 min three times to ensure breathing occurred. Absolute and mass-specific VCO₂, total activity, body mass, and ventilation patterns were compared among species. As expected, ticks did not always breathe during the 30-minute measurements, especially <i>R. sanguineus</i>. Ventilation patterns differed among species with <i>R. microplus</i> having primarily cyclic patterns and <i>R. appendiculatus</i> having discontinuous gas exchange. VCO₂ did not scale with body mass in most species. <i>Haemaphysalis longicornis</i> and <i>R. sanguineus</i> had the lowest VCO₂; however, <i>H. longicornis</i> was the second most active species. Life history, including questing behavior and range expansion, could be contributing to differences between species. For instance, <i>H. longicornis</i> had exceptionally low metabolic rates despite above average activity levels, suggesting an energetic advantage which may underlie recently documented range expansions in North America. Our results demonstrate how ticks utilize energetic resources to maximize longevity. Future research describing questing behavior and distribution modeling may help explain differences in metabolic rates and activity and impacts on life history traits.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"1 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo assembly of sialotranscriptome of Hyalomma anatolicum and insights into expression dynamics in response to Theileria annulata infection","authors":"Adeel Mumtaz Abbasi, Shiza Nasir, Amna Arshad Bajwa, Haroon Akbar, Sara Artigas-Jerónimo, Clara Muñoz-Hernández, Marta Sánchez-Sánchez, Alberto Moraga-Fernández, Isabel G. Fernández de Mera, José de la Fuente, Muhammad Imran Rashid","doi":"10.1007/s10493-024-00962-z","DOIUrl":"https://doi.org/10.1007/s10493-024-00962-z","url":null,"abstract":"<p><i>Hyalomma anatolicum</i> is a tick of significant one-health importance due to its role as a vector for various pathogens affecting humans, animals and the environment, such as <i>Theileria annulata</i>, which causes tropical theileriosis in cattle, leading to severe economic losses. When infected with pathogens like <i>T. annulata</i>, the salivary glands of <i>H. anatolicum</i> undergo gene expression changes, secrete modified proteins and activate immune responses, all of which facilitate pathogen survival and transmission by modulating the host immune response and optimizing conditions for pathogen development. Understanding these responses is crucial for developing control strategies for tick-borne diseases. To understand the interaction between <i>H. anatolicum</i> and <i>T. annulata,</i> we performed a differential gene expression analysis of <i>H. anatolicum</i> salivary glands. An average of approximately 25 million raw sequencing reads were generated in each replicate using Illumina Sequencing. The sequenced reads were de novo assembled and the assembled transcriptome yielded approximately 50,231 non-redundant transcripts after clustering with CD-HIT using a sequence identity of 95% and alignment coverage of 90%. The assembly quality was evaluated with BUSCO analysis and found to be 86% complete using the Arachnida dataset and then blasted against non-redundant protein sequence database from NCBI followed by counting of reads and differential expression analysis. Overall, around 2400 and 400 genes were found differentially expressed with logFC &gt; 1 and logFC &gt; 2 respectively at FDR &lt; 0.05. Top up-regulated genes included Calpain, Papilin, Neprilysin, and Ankyrin repeat-containing protein. Top down-regulated genes included Scoloptoxin, and Selenoprotein S and other uncharacterized proteins. Many other up-regulated proteins with high significance were uncharacterized suggesting room for further <i>H. anatolicum</i> functional and structural characterization studies. To our best knowledge, this is the first study of <i>H. anatolicum</i> sialotranscriptome which greatly contributes to sialotranscriptome information not only as sequence database but also indicates the potential targets for development of vaccine against ticks and transmission-blocking vaccines against <i>T. annulata</i>.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"19 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of variation in plant-emitted volatiles on the infestation behavior of Tetranychus urticae (Acari: Tetranychidae) and Frankliniella occidentalis (Thysanoptera: Thripidae) in strawberry crops","authors":"Neby M. Mérida-Torres, Edith Garay-Serrano, Samuel Cruz-Esteban","doi":"10.1007/s10493-024-00963-y","DOIUrl":"https://doi.org/10.1007/s10493-024-00963-y","url":null,"abstract":"<p>The two-spotted spider mite (TSSM), <i>Tetranychus urticae</i> Koch, and the Western flower thrips (WFT), <i>Frankliniella occidentalis</i> (Pergande), are pests commonly found in strawberry crops and pose significant challenges to production. However, the specific dynamics of their interactions with both healthy and infested plants remain poorly understood. In this study, we aimed to investigate the attraction of TSSM and WFT to volatile compounds emitted by healthy plants versus those of plants damaged by either or both species. Plant choice bioassays were conducted under varying conditions, including both healthy and those previously damaged by both TSSM and WFT. Additionally, behavioral tests were carried out using a Y-tube olfactometer, with extracts obtained via dynamic aeration from the plants in different states. The results revealed distinct preferences: TSSM exhibited a strong attraction to both healthy plants and those previously infested by their own specifics, whereas WFT showed a higher preference for healthy plants and those damaged by TSSM. Consistent behaviors were observed in the bioassays conducted with plant extracts. This research sheds light on the intricate interactions between strawberry plants and these pests and offers insights into the probable sequence of attack when both pests are present concurrently. The findings are valuable when implementing management strategies for these two pests in strawberry cultivation, considering the order in which they appear in the crop to help mitigate the damage caused by infestation in a more precise manner and order.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"27 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142204798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}