Experimental and Applied Acarology最新文献_第4页

Seach for tick-borne agents and detection of a novel Ehrlichia sp. in ticks from wild rabbits in Northern Spain. 在西班牙北部寻找蜱传病原体并在野兔蜱中发现一种新型埃利希氏体。

IF 1.7 2区农林科学

Experimental and Applied Acarology Pub Date : 2025-07-09 DOI: 10.1007/s10493-025-01046-2

Igor Silva Silito, Matheus Pasini Martins, Thallitha Samih Wischral Jayme Vieira, Maria Carolina A Serpa, Isabella Pereira Pesenato, Agustin Estrada-Peña, Marcelo B Labruna

{"title":"Seach for tick-borne agents and detection of a novel Ehrlichia sp. in ticks from wild rabbits in Northern Spain.","authors":"Igor Silva Silito, Matheus Pasini Martins, Thallitha Samih Wischral Jayme Vieira, Maria Carolina A Serpa, Isabella Pereira Pesenato, Agustin Estrada-Peña, Marcelo B Labruna","doi":"10.1007/s10493-025-01046-2","DOIUrl":"10.1007/s10493-025-01046-2","url":null,"abstract":"This study aimed to investigate tick-borne agents in ticks (Ixodida: Ixodidae) collected from wild rabbits (Oryctolagus cuniculus) hunted in northern Spain in 2022. A total of 120 ticks were collected from 11 rabbits (mean: 13.7 ticks/rabbit), and identified as 109 Rhipicephalus pusillus and 11 Haemaphysalis hispanica. DNA was extracted from 89 R. pusillus and 5 H. hispanica and tested by a battery of PCR assays targeting tick-borne bacteria (genera Rickettsia, Borrelia, Coxiella, family Ehrlichiaceae) and protozoa (Piroplasmida). All ticks were negative for these agents, except for two specimens (one H. hispanica and one R. pusillus) that yielded amplicons by the Ehrlichiaceae 16 S rRNA assay. These two tick specimens were further tested by PCR assays targeting fragments of the ehrlichial genes groEL and dsb, which were generated only from H. hispanica. The groEL haplotype showed the closest identity (95%) with over 30 Ehrlichia haplotypes, while the dsb haplotype showed closest identity (88%) with 'Candidiatus Ehrlichia pampeana'. Phylogenetic analyses inferred from the groEL, and dsb partial sequences indicated that the ehrlichial agent detected in a H. hispanica male- here designated as Ehrlichia sp. Hhisp1- is a novel agent, distinct from all ehrlichial agents with available sequences in GenBank. Our finding of Ehrlichia sp. Hhisp1 in H. hispanica points out the possibility that the wild rabbit could be a reservoir of this novel ehrlichial agent since there is no transovarial transmission of Ehrlichia spp., and H hispanica is highly specific to wild rabbits and their burrows.","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 2","pages":"15"},"PeriodicalIF":1.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scrutinizing Acalitus simplex Flechtmann et Etienne (Eriophyidae): an eriophyoid mite associated with Ruellia spp. (Acanthaceae). 细察单角螨Flechtmann et Etienne（棘螨科）：与棘螨科Ruellia属相关的一种棘螨。

IF 1.7 2区农林科学

Experimental and Applied Acarology Pub Date : 2025-07-09 DOI: 10.1007/s10493-025-01040-8

Marcello De Giosa, Livia M S Ataide, Adam G Dale, Daniel A Hahn, Xingbo Wu, Ronald Ochoa, Samuel Bolton, Kevin R Cloonan, Alexandra M Revynthi

{"title":"Scrutinizing Acalitus simplex Flechtmann et Etienne (Eriophyidae): an eriophyoid mite associated with Ruellia spp. (Acanthaceae).","authors":"Marcello De Giosa, Livia M S Ataide, Adam G Dale, Daniel A Hahn, Xingbo Wu, Ronald Ochoa, Samuel Bolton, Kevin R Cloonan, Alexandra M Revynthi","doi":"10.1007/s10493-025-01040-8","DOIUrl":"10.1007/s10493-025-01040-8","url":null,"abstract":"Acalitus simplex is an erinose eriophyoid mite that feeds exclusively on plants in the genus Ruellia, which are frequently sold as ornamentals for landscape plantings. This study presents a comprehensive examination of A. simplex collected from Anguilla, Brazil, Dominican Republic, Florida, and Hawaii through sequencing of the mitochondrial cytochrome oxidase I gene (COI), coupled with morphological examination by several microscopy techniques. The collected A. simplex specimens were similar in morphology across geographic locations and populations clustered together in a phylogenetic analysis based on COI sequences. In addition to Ruellia simplex, new host plant associations are reported in Florida, including R. blechum, R. squarrosa and R. caroliniensis. We defined the different erineum stages, determined the number of days after infestation on an uninfested plant required for erinea development, and estimated population densities in each erineum stage. Four erineum stages were observed on R. simplex - hyaline (stage 1), white (stage 2), purple (stage 3) and beige (stage 4). At 25 ± 2 oC, 12:12/L: D and 50 ± 10% RH, the purple stage did not develop and the duration of the hyaline, white and beige erinea was 12.4 ± 0.7 (mean ± SE), 44.4 ± 1.3, and 20.5 ± 0.9 days, respectively. Population densities varied significantly across the erineum stages, with most individuals in the white and purple stages and fewer in the hyaline and beige stages. This study improves our understanding of interactions between A. simplex and R. simplex, aiding the development of IPM strategies to mitigate this mite.","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 2","pages":"13"},"PeriodicalIF":1.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disentangling the effect of tillage, herbicide, pesticide, and temperature change on oribatid mite communities in Georgia. 分离耕作、除草剂、杀虫剂和温度变化对乔治亚州甲螨群落的影响。

IF 1.7 2区农林科学

Experimental and Applied Acarology Pub Date : 2025-06-12 DOI: 10.1007/s10493-025-01033-7

Maka Murvanidze, Mark Maraun, Jing-Zhong Lu, Levan Mumladze, Nino Todria, Tea Arabuli, Meri Salakaia, Giorgi Kirkitadze

{"title":"Disentangling the effect of tillage, herbicide, pesticide, and temperature change on oribatid mite communities in Georgia.","authors":"Maka Murvanidze, Mark Maraun, Jing-Zhong Lu, Levan Mumladze, Nino Todria, Tea Arabuli, Meri Salakaia, Giorgi Kirkitadze","doi":"10.1007/s10493-025-01033-7","DOIUrl":"10.1007/s10493-025-01033-7","url":null,"abstract":"Agricultural practices affect non-target soil fauna either directly or indirectly, e.g. by altering the soil physical structure or by application of chemicals. The effects of tillage, insecticide and herbicide applications on the dominant taxon of soil microarthropods-oribatid mites was studied in two fields over three years (2020, 2021, 2022) at three seasons (spring, summer and autumn). In total 87 species were identified. Herbicide and insecticide application had negative effects on oribatid mite species numbers and abundance; however, tillage mitigated the negative effects of herbicide and insecticide applications, possibly since tillage increases the rate of pesticide degradation and thereby reduces the direct exposure to soil fauna to those chemicals. Insecticides and tillage reduced the number of parthenogenetic individuals, possibly by a decrease of available resources (dead organic matter). Oribatid species richness and density steadily declined from 2020 to 2022 likely due to increased average annual temperature and decreased precipitation associated with global warming. The species richness and density declined from spring to summer and increased again in autumn. The annual decline of the oribatid species richness was expressed by the loss of oribatids with a thin cuticle (Mixonomata, Enarthronota, Oppioidea) and those, who are sensitive towards disturbance (Liacaridae, Ceratozetidae). Dry grassland specific species (Passalozetes africanus, P. perforatus, Scutovertex sculptus) were constantly present in all samples. The study points to the effects of agricultural practices on non-target soil fauna and also on the changes in soil animal communities from meadow to dry steppe species due to global warming.","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 2","pages":"12"},"PeriodicalIF":1.7,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Essential oils as feed additives: A novel approach to managing poultry red mite infestations and boosting egg production. 作为饲料添加剂的精油：一种管理家禽红螨侵扰和提高产蛋率的新方法。

IF 1.7 2区农林科学

Experimental and Applied Acarology Pub Date : 2025-06-11 DOI: 10.1007/s10493-025-01035-5

Jasna Bošnjak-Neumüller, Luis-Miguel Gomez Osorio, Dajana Todorović, Larisa Ilijin, Vesna Perić-Mataruga, Nikola Delić, Aleksandar Stanojković, Marko Pajić, Slobodan Kenežević, Jog Raj, Marko Vasiljević, Olivier Sparagano

{"title":"Essential oils as feed additives: A novel approach to managing poultry red mite infestations and boosting egg production.","authors":"Jasna Bošnjak-Neumüller, Luis-Miguel Gomez Osorio, Dajana Todorović, Larisa Ilijin, Vesna Perić-Mataruga, Nikola Delić, Aleksandar Stanojković, Marko Pajić, Slobodan Kenežević, Jog Raj, Marko Vasiljević, Olivier Sparagano","doi":"10.1007/s10493-025-01035-5","DOIUrl":"10.1007/s10493-025-01035-5","url":null,"abstract":"This study assessed the efficacy of a plant-based premix of feed additives (PFA) composed of essential oils and vitamins on a farm with 1560 laying hens (ISA Brown line), naturally infested with poultry red mite (PRM), Dermanyssus gallinae. The trial lasted 44 days. The AVIVET traps were used to determine PRM mass, number of eggs, larvae, blood-engorged and unfed nymphs, and adults on day - 12, - 5, and 0 before, and on days 2, 5, 8, 11, 15, 23, and 30, after, hens started to consume PFA. Friedman's ANOVA was utilised to identify differences between means, and the post hoc Wilcoxon matched Pairs Test was then employed to ascertain the impact of the tested PFA on the D. gallinae population. From the eighth day until the end of the trial period, a statistically lower mass of mites (P < 0.05), the number of nymphs (P < 0.05) and the number of blood-engorged mites (P < 0.05) were observed compared to day 0. In comparison to day 0, a statistically significantly lower number of eggs (P < 0.01) was observed on day 5 (P < 0.05), day 11 (P < 0.01), larvae on day 23 (P < 0.05) and day 30 (P < 0.01), as well as unfed mites on days 15, 23, and 30 (P < 0.01, P < 0.01 and P < 0.001, respectively). Egg production (%, hen-day) in 52-54 weeks of age (control period) was 88.7, while in 55-58 weeks of age (trial period) was 89.5. The results indicate that the tested PFA can control PRM without adversely affecting egg production.","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 1","pages":"10"},"PeriodicalIF":1.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and genetic discrepancy of Rhipicephalus sp. (Acari: Ixodidae) collected from the ricefield rat, Rattus argentiventer, in Peninsular Malaysia. 马来西亚半岛稻田大鼠阿根廷家鼠（Rattus argentiventer）棘头螨属（蜱螨亚纲：伊蚊科）形态与遗传差异。

IF 1.7 2区农林科学

Experimental and Applied Acarology Pub Date : 2025-06-11 DOI: 10.1007/s10493-025-01036-4

Abdul Rahman Kazim, Van Lun Low, Tiong Kai Tan, Vinnie-Siow Wei Yin, Ariff Ateed Mohd Noh, Chong Chin Heo, Dzulhelmi Muhammad Nasir

引用次数: 0

Investigating interspecific mating in the thelytokous predatory mite Amblyseius herbicolus (Chant) (Acari: Phytoseiidae), with comparative observations from three sexually reproducing phytoseiid species. 研究了植塞螨（蜱螨亚纲：植塞螨科）的种间交配，并对三种有性繁殖的植塞螨进行了比较。

IF 1.7 2区农林科学

Experimental and Applied Acarology Pub Date : 2025-06-03 DOI: 10.1007/s10493-025-01034-6

Keshi Zhang, Junlin Cao, Xintong Li, Zhi-Qiang Zhang

{"title":"Investigating interspecific mating in the thelytokous predatory mite Amblyseius herbicolus (Chant) (Acari: Phytoseiidae), with comparative observations from three sexually reproducing phytoseiid species.","authors":"Keshi Zhang, Junlin Cao, Xintong Li, Zhi-Qiang Zhang","doi":"10.1007/s10493-025-01034-6","DOIUrl":"10.1007/s10493-025-01034-6","url":null,"abstract":"Asexual reproduction can be advantageous in the short term but is generally considered an evolutionary dead end due to the lack of genetic diversity and the accumulation of deleterious mutations. The thelytokous predatory mite Amblyseius herbicolus (Chant) (Acari: Mesostigmata) shows potential for biological pest control, but its reproductive mechanism remains poorly understood. This study examined whether A. herbicolus females have retained mating ability by exposing them to morphologically similar heterospecific males from three sexually reproducing phytoseiid species: Amblydromalus limonicus (Garman & McGregor), Amblyseius lentiginosus Denmark & Schicha, and Neoseiulus cucumeris (Oudemans). No mating or insemination occurred between A. herbicolus females and heterospecific males within 24 h. In contrast, nearly all males successfully inseminated conspecific females in a follow-up observation. Mating behaviours varied across species, with A. lentiginosus showing the longest pre-mating duration and lowest insemination rate, indicating higher mate selectivity. Most Ad. limonicus males inseminated a single spermatophore, while A. lentiginosus and N. cucumeris deposited two spermatophores, suggesting differences in sperm allocation and competition. No correlation was found between male body size, mating duration, and endospermatophore volume, possibly due to the small sample size. This study does not establish whether A. herbicolus reproduces strictly through thelytoky, nor does it exclude the possibility of sexually reproducing populations elsewhere. Further research, including interspecific mating trials with the more morphologically similar Amblyseius largoensis and an investigation into the role of endosymbionts such as Wolbachia, is needed to reveal the mechanisms underlying asexuality in A. herbicolus.","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 1","pages":"8"},"PeriodicalIF":1.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12133926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitogenomic evidence for the monophyly of blackcurrant gall mite subfamily Cecidophyinae (Eriophyoidea, Eriophyidae). 黑加仑瘿螨亚科（瘿螨总科，瘿螨科）单系的有丝分裂学证据。

IF 1.7 2区农林科学

Experimental and Applied Acarology Pub Date : 2025-06-03 DOI: 10.1007/s10493-025-01027-5

Vladimir D Gankevich, Philipp E Chetverikov

{"title":"Mitogenomic evidence for the monophyly of blackcurrant gall mite subfamily Cecidophyinae (Eriophyoidea, Eriophyidae).","authors":"Vladimir D Gankevich, Philipp E Chetverikov","doi":"10.1007/s10493-025-01027-5","DOIUrl":"10.1007/s10493-025-01027-5","url":null,"abstract":"Superfamily Eriophyoidea is a group of highly miniaturized and host-specific phytophagous acariform mites closely related to the soil-dwelling nematalycid mites. Limited number of reliable morphological characters, multiple homoplasies, and numerous cryptic species impede systematics of this group. Most recent multigene phylogenies of Eriophyoidea suffer from incomplete sampling, inclusion of erroneous sequences, and unreliable alignments resulting in biologically inconsistent results. In this study, we obtained five new mitogenomes of eriophyoids and reanalyzed the mitogenomic dataset by Zhang et al. (2024) in order to test their conclusion on the polyphyly of the subfamily Cecidophyinae. We found out that it was based on the wrong identification of the phyllocoptine sequence OQ615736 as a cecidophyine taxon. Our analysis showed that a group of seven mitogenomic sequences assigned to cecidophyine genera Cecidophyes, Cecidophyopsis, Coptophylla, Cosetacus, and Glyptacus form a monophyletic group, which was itself divided into two clades corresponding to previously recognized tribes Colomerini and Cecidophyini. However, no mitochondrial gene clusters unique to Cecidophyinae were found. Gene order variability in Cecidophyinae is low and concerns only positions of the control region and tRNA genes trnC and trnI. Analysis of nucleotide diversity showed that three mitochondrial protein coding genes (COX1, COX3 and CYTB) are promising molecular markers for future eriophyoid studies, whereas genes ATP8, NAD4L, NAD5, NAD6 are hypervariable and should be used with caution or even excluded (ATP8) from multigene phylogenetic analyses. Finally, we identified numerous sequences of Eriophyoidea in GenBank with incorrect taxonomic affinities, underscoring the urgent need for a comprehensive revision of all eriophyoid mite sequences deposited in public databases.","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 1","pages":"9"},"PeriodicalIF":1.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial diets for rearing Euseius scutalis (Acari: Phytoseiidae). 养殖绢金拟蚊的人工饲料（蜱螨目：植螨科）。

IF 1.7 2区农林科学

Experimental and Applied Acarology Pub Date : 2025-05-28 DOI: 10.1007/s10493-025-01030-w

Fereshteh Bazazzadeh, Parviz Shishehbor, Seyed Ali Hemmati, Elham Riahi

{"title":"Artificial diets for rearing Euseius scutalis (Acari: Phytoseiidae).","authors":"Fereshteh Bazazzadeh, Parviz Shishehbor, Seyed Ali Hemmati, Elham Riahi","doi":"10.1007/s10493-025-01030-w","DOIUrl":"10.1007/s10493-025-01030-w","url":null,"abstract":"Mass rearing of the predatory mite Euseius scutalis (Athias-Henriot) using natural prey is costly and labor-intensive, limiting its application in biological pest control. Artificial diets may serve as alternative food sources; however, some studies should be previously conducted, to evaluate the possible biological consequences of their adoption. In this study, we compared the suitability of eight artificial diets, including a basic artificial diet (A) consisting of a combination of 10% honey, 10% sucrose, 5% tryptone, 5% yeast extract, 10% egg yolk, and 60% distilled water (w/w), along with seven other diets, each consisting of 80% of diet A enriched with date palm pollen (B), Typha pollen (C), Ephestia kuehniella eggs (D), Helicoverpa armigera hemolymph (E), a mixture of date palm pollen and E. kuehniella eggs (F), Tetranychus turkestani adults (G), and hen's liver (H). The data showed that E. scutalis was able to complete its development on all tested diets. Oviposition of the predator was observed on all diets, except for diets E and H. Females reared on diets B and F had the shortest adult pre-oviposition period (APOP), while those on diet B had the shortest total pre-oviposition period (TPOP). Furthermore, the number of oviposition days and fecundity were highest on diets B, D and F. Diets B and F resulted in significantly higher intrinsic (r) and finite (λ) rates of increase than the other diets, with values statistically similar to those obtained on D. The lowest values of r and λ were recorded on diet G. Based on our results, date palm pollen, E. kuehniella eggs or their mixture added to the basic diet A are more beneficial than other options for use in artificial diets for the mass rearing of E. scutalis.","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 1","pages":"7"},"PeriodicalIF":1.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144157438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First records of Amblyomma geayi and Amblyomma varium (Ixodida: Ixodidae) parasitizing sloths in Nicaragua. 尼加拉瓜树懒寄生性双足线虫和大双足线虫的首次记录。

IF 1.7 2区农林科学

Experimental and Applied Acarology Pub Date : 2025-05-28 DOI: 10.1007/s10493-025-01032-8

Juan J Oporta-López, Carlos Eduardo Molina Vargas, Carmen Guzmán-Cornejo, Adriana Troyo, Filipe Dantas-Torres

引用次数: 0

The dual role of the RR-2 cuticular protein gene in development and acaricide susceptibility of Panonychus citri (Acari: Tetranychidae). r -2表皮蛋白基因在柑橘全爪螨发育和杀螨敏感性中的双重作用。

IF 1.7 2区农林科学

Experimental and Applied Acarology Pub Date : 2025-05-27 DOI: 10.1007/s10493-025-01029-3

Haifeng Wang, Tianrong Xin, Chengyu Jiang, Hongyan Wang, Bin Xia

{"title":"The dual role of the RR-2 cuticular protein gene in development and acaricide susceptibility of Panonychus citri (Acari: Tetranychidae).","authors":"Haifeng Wang, Tianrong Xin, Chengyu Jiang, Hongyan Wang, Bin Xia","doi":"10.1007/s10493-025-01029-3","DOIUrl":"10.1007/s10493-025-01029-3","url":null,"abstract":"Panonychus citri, a major pest mite globally, causes substantial economic damage in the citrus sector. Due to the prolonged use of acaricides, P. citri has developed resistance; thus, identifying alternative control measures is imperative. The study successfully cloned and characterized three genes from the RR-2 subfamily of cuticular proteins (CP) in P. citri, PcCPR14, PcCPR15.7, and PcCPR16.8. These genes exhibited their highest expression in eggs aged 4 and 5 days and showed relatively high expression in larvae and nymphs on day 1. Inhibitin PcCPR16.8 expression in eggs resulted in only 23% hatching success. Similarly, silencing PcCPR16.8 is inhibited in nymphs led to cuticle rupture and a mortality rate of 81.77%. Silencing in female mites reduced the oviposition rate by 31.06% and significantly decreased chitin content and the expression of key chitin metabolism genes in P. citri. This indicates the crucial role of CP genes in the development and reproduction of P. citri. Moreover, exposure to the acaricides spirobudiclofen and etoxazole typically triggers high expression of PcCPR16.8, and silencing this gene increases the mite's sensitivity to these chemicals. CPs are vital in defending against environmental changes, making them potential targets for developing P. citri control strategies.","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 1","pages":"5"},"PeriodicalIF":1.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0