Experimental and Applied Acarology最新文献

{"title":"Life table parameters of Phytonemus pallidus fragariae Zimmerman (Acari: Tarsonemidae) on different strawberry cultivars: A comparative study.","authors":"Mete Soysal, Rana Akyazi","doi":"10.1007/s10493-025-01049-z","DOIUrl":"10.1007/s10493-025-01049-z","url":null,"abstract":"<p><p>Phytonemus pallidus fragariae Zimmerman (Trombidiformes: Tarsonemidae) is an important pest in strawberry cultivation worldwide, causing considerable economic losses with yield reductions ranging from 22 to 100%. This study investigated the population parameters of P. p. fragariae on three commercially important strawberry cultivars: Albion, Monterey, and San Andreas, using an age-stage, two-sex life table approach. All experimental procedures were conducted under the controlled conditions of 25 ± 2 °C, 65 ± 5% RH, and a 16:8 h (light: dark) photoperiod. The results showed that some developmental times and life table parameters of P. p. fragariae differed among the cultivars. Total pre-adult duration was longest on Albion (8.23 days), followed by Monterey (7.65 days) and San Andreas (7.10 days). Fecundity was highest on Albion (11.85 eggs/female), while no differences were observed in net reproductive rate, intrinsic rate of increase, and finite rate of increase among cultivars. However, the mean generation time was longest on Albion (13.85 days) and shortest on San Andreas (12.10 days). The findings indicate that the Albion cultivar is more suitable for development and reproduction. P. p. fragariae than the Monterey and San Andreas cultivars. This study provides initial insights into the life table parameters of P. p. fragariae on specific strawberry cultivars, enhancing our understanding of pest-host interactions. The data generated helps develop more effective integrated pest management (IPM) strategies targeting this pest in strawberry production systems.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 2","pages":"23"},"PeriodicalIF":1.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Mitogenomic evidence for the monophyly of blackcurrant gall mite subfamily Cecidophyinae (Eriophyoidea, Eriophyidae).","authors":"Vladimir D Gankevich, Philipp E Chetverikov","doi":"10.1007/s10493-025-01038-2","DOIUrl":"10.1007/s10493-025-01038-2","url":null,"abstract":"","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 2","pages":"24"},"PeriodicalIF":1.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional analysis of Mu-class glutathione S-transferase HdGSTm1 from Haemaphysalis doenitzi with a focus on the roles in the tick blood sucking.","authors":"Xiaolong Yang, Kuang Wang, Meichen Zhao, Feidi Guo, Runying Wang, Jianing Liu, Zhihua Gao","doi":"10.1007/s10493-025-01037-3","DOIUrl":"10.1007/s10493-025-01037-3","url":null,"abstract":"<p><p>During blood feeding, ticks face significant oxidative stress due to hemoglobin breakdown. In this context, GSTs are proposed to act as critical antioxidants, possibly by detoxifying lipid peroxides. The transcriptome of H. doenitzi was screened for GST genes, followed by the recombinant expression and functional analysis of these genes. Specifically, HdGSTm1 was cloned by PCR, and the recombinant protein rHdGSTm1 was expressed and purified. The properties of rHdGSTm1 were analyzed, gene transcription was assessed, and RNA interference was employed to evaluate its functional impact. As results, the open reading frame of HdGSTm1 is 693 base pairs (bp) in length and number of coding amino acids is 230 and it belongs to Mu-class GSTs. And the activity of GST for GSH was measured (V_max =1.39 ± 0.07 µmol/(min·mg), K_m=1.254 ± 0.12 µM). It has the highest enzyme activity at pH 7.0, 30 ℃. Antioxidant assay showed that rHdGSTm1 had concentration-dependent antioxidant capacity. The relative expression of HdGSTm1 was higher in adult ticks and the highest in Malpighian tubules. RNAi results demonstrated that HdGSTm1 had a significant effect on bloodsucking (t-test, t₍₃₄₎ = 4.296, p = 0.0001). In summary, this study isolated and characterized a Mu-class GST gene from H. doenitzi. The recombinant protein exhibits free radical scavenging activity. Evaluation of its antioxidant capacity facilitates analysis of the active oxidative stress response mechanism during blood feeding in adult Malpighian tubules, and provides a basis for elucidating the molecular mechanism. Notably, it impacts blood feeding behavior. HdGSTm1, even at low expression levels, can significantly reduce the blood-feeding duration in H. doenitzi.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 2","pages":"22"},"PeriodicalIF":1.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astigmatid mites (Acari: Astigmata) as factitious prey for mass rearing Amblyseius largoensis (Acari: Phytoseiidae).","authors":"João M R Costa, Débora B Lima, Manoel G C Gondim, José E M de Oliveira, José W S Melo","doi":"10.1007/s10493-025-01039-1","DOIUrl":"10.1007/s10493-025-01039-1","url":null,"abstract":"<p><p>Predatory mites, particularly those of the Phytoseiidae family, play a key role in sustainable pest management by reducing pest populations and minimizing reliance on chemical pesticides. Amblyseius largoensis (Muma) (Acari: Phytoseiidae) is a generalist predator, with great potential for controlling noxious agricultural pests. However, large-scale rearing of A. largoensis remains a challenge due to the lack of an efficient mass-production system. This study aimed to select a factitious prey species from the order Astigmata that could support the mass-rearing of A. largoensis. Four Astigmata species (Carpoglyphus lactis L., Thyreophagus crasentiseta Barbosa, OConnor & Moraes, Tyrophagus putrescentiae Schrank, and Glycycometus aff. molitor Volgin & Akimov) were evaluated for their ability to sustain the predator's survival, development, and reproduction. Tetranychus urticae Koch, a well-established natural prey, was used as a control. Bioassays assessed the growth rates of A. largoensis when fed exclusively on each prey species. The results showed that C. lactis supported survival and reproduction rates comparable to T. urticae, with an intrinsic growth rate favorable for mass-rearing. The other prey proved unsuitable for A. largoensis mass-rearing. Additionally, C. lactis offers practical advantages as it is easy to rearing, thrives on simple and inexpensive food sources, and allows for continuous predator production. This study highlights the potential of C. lactis as a viable factitious prey for the large-scale production of A. largoensis, contributing to the diversification of biological control strategies. Future research should focus on optimizing rearing protocols and field evaluation to validate the effectiveness of A. largoensis as a biocontrol agent in tropical and subtropical regions.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 2","pages":"21"},"PeriodicalIF":1.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transgenerational phenotypic plasticity of the two-spotted spider mite (Tetranychus urticae Koch) in response to abamectin.","authors":"T Martinelli, M Lauria, I Mascheretti, D Goggioli, S Guidi, F Turillazzi, E Gagnarli, F Tarchi, P F Roversi, S Simoni","doi":"10.1007/s10493-025-01042-6","DOIUrl":"10.1007/s10493-025-01042-6","url":null,"abstract":"<p><p>Tetranychus urticae Koch, commonly known as the two-spotted spider mite, is a serious threat to many crop species. This species can rapidly develop resistance to acaricides, rendering chemical control of the mite extremely difficult. In the present study, a homozygous mite population of T. urticae was established, and the potential involvement of transgenerational phenotypic plasticity in the development of resistance to the acaricide abamectin was investigated. The mite methylome was analyzed for the first time to evaluate the possible role of this epigenetic mark in the observed response to abamectin. The results showed a significant maladaptive transgenerational phenotypic response. Mites, previously exposed to abamectin treatments for seven consecutive generations, showed lower acaricide resistance compared to mites that were never treated with abamectin. This phenotype persisted for two consecutive generations after the termination of abamectin exposure. Methylome analysis of treated and untreated mite groups revealed that this species possessed an extremely low level of cytosine methylation, if any, suggesting that this epigenetic mark is unlikely to be involved in the transgenerational response. Overall, the results show that transgenerational phenotypic plasticity can significantly modify the phenotype of T. urticae. However, under the conditions tested, transgenerational phenotypic plasticity is not directly involved in the development of abamectin resistance. Further work is needed to better understand how transgenerational phenotypes are involved in the response of T. urticae to acaricides.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 2","pages":"20"},"PeriodicalIF":1.7,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in the effects of pollen and prey-based diets on the biological performance of Euseius citrifolius (Acari: Phytoseiidae): an important predator for phytophagous mites management in grapevines.","authors":"Cleiton A Domingos, André S Melo, Maria L T Matheus, José E M Oliveira, José W S Melo, Manoel G C Gondim","doi":"10.1007/s10493-025-01044-4","DOIUrl":"10.1007/s10493-025-01044-4","url":null,"abstract":"<p><p>Euseius citrifolius Denmark & Muma is a type IV generalist predatory mite with a dietary preference for pollen. In this study, we tested the hypothesis that different prey, even if phylogenetically related and reared on the same host, can influence the predator's fitness by affecting its development and reproduction. We evaluated the biological performance of E. citrifolius when fed on Ricinus communis pollen and prey-based diets. Prey-based diets consisted of Oligonychus punicae and Tetranychus urticae reared on grapevines, as well as pollen. The following parameters of E. citrifolius were evaluated: developmental time of immature stages, longevity, reproduction, cumulative percentage of adults, mean oviposition rate, life-table parameters, and fertility. Oligonychus punicae and T. urticae diets resulted in a longer development time from egg to adult, increased longevity, higher oviposition rate, higher cumulative percentage of adults, and higher daily oviposition than the pollen diet. Although the pollen diet was less efficient at reproduction, it promoted higher viability. The net reproduction rate, intrinsic rate of increase, and mean generation time were higher in prey-based diets (O. punicae and T. urticae) than in the pollen diet. Overall, both prey diets supported the biological performance of E. citrifolius. These findings provide valuable insights for future research on the mass production of E. citrifolius and its potential for controlling O. punicae and T. urticae in grapevines.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 2","pages":"16"},"PeriodicalIF":1.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From mites to migration: linking Spinturnix psi morphometrics to the conservation of its host, the common bent-winged bat (Miniopterus schreibersii).","authors":"Jelena Burazerović, Katarina Breka, Marija Jovanović, Zvezdana Jovanović","doi":"10.1007/s10493-025-01045-3","DOIUrl":"10.1007/s10493-025-01045-3","url":null,"abstract":"<p><p>Geometric morphometrics has contributed significantly to understanding ecological and evolutionary patterns by analyzing shape variation in biological structures, including species identification, population differentiation, and phenotypic plasticity of different organisms. In the context of bat ectoparasites, particularly wing mites, geometric morphometrics could present a novel approach for tracking the migratory patterns of bat hosts over time. To evaluate the morphological variation of Spinturnix psi (Kolenati, 1856) mites sampled from its typical host, Miniopterus schreibersii (Kuhl, 1817), in the three caves in eastern Serbia (Bogovinska, Sokolovica, and Sesalačka caves), we applied geometric morphometrics methods. We selected the sternal shield as the character to be analyzed and positioned 10 landmarks to describe its shape in females and 20 landmarks in males respectively. The analysis revealed statistically significant shape differences in the males, where a clear differentiation of specimens from Bogovinska cave compared to specimens from the other two localities was found, while in the females no differences were observed. Nevertheless, pairwise comparisons showed a weak but significant signal indicating differences between females from Bogovinska and Sesalačka caves. These subtle morphological variation in mites could help clarify the links between different host roosting sites, and a better understanding of the migration patterns of bats.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 2","pages":"18"},"PeriodicalIF":1.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative fitness of Proprioseiopsis asetus (Acari: Phytoseiidae) as a biocontrol agent against Megalurothrips usitatus and Thrips flavus.","authors":"Hui Zhang, Li Sun, Shuo Lin, Yuxian He, Hui Wei, Xia Chen","doi":"10.1007/s10493-025-01047-1","DOIUrl":"10.1007/s10493-025-01047-1","url":null,"abstract":"<p><p>Proprioseiopsis asetus (Chant) (Acari: Phytoseiidae) is a newly developed and commercialized predatory mite that can prey on various phytophagous mites and small insects. To evaluate its potential as a biocontrol agent against pest thrips (Thysanoptera: Thripidae), we constructed age-stage, two-sex life tables and investigated the biological characteristics of P. asetus fed on two economically important thrip species, Megalurothrips usitatus and Thrips flavus (Thysanoptera: Thripidae), both of which cause significant crop damage. Our results show that P. asetus successfully develops and reproduces when fed on both thrips species, indicating its biocontrol potential against these pests. However, P. asetus reared on T. flavus exhibits shorter preadult duration, mean generation time (T), and population doubling time (DT), along with obviously reduced longevity, compared to P. asetus reared on M. usitatus. Despite the shorter lifespan, P. asetus fed on T. flavus shows significantly higher fecundity (F = 36.35 eggs/female), intrinsic rate of increase (r = 0.1816 d^-1) and finite rate of increase (λ = 1.1991 d^-1) than on M. usitatus (F = 30.75 eggs/female, r = 0.1375 d^-1 and λ = 1.1474 d^-1). Notably, no significant differences were observed in net reproductive rate (R₀), adult preoviposition period (APOP), total oviposition period (TPOP), or oviposition duration between the two prey species. These findings indicate that P. asetus is a promising biological control agent for both thrip species, with T. flavus being a more suitable prey to support its rapid development, high fecundity and faster population growth, despite the trade-off in adult longevity. Based on these results, we propose tailored biocontrol strategies: frequent releases of P. asetus for T. flavus management to compensate for its shorter lifespan, and early release for M. usitatus to establish a population before the pest reaches damaging levels, aiming for more efficient and sustainable pest management in crop production systems.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 2","pages":"17"},"PeriodicalIF":1.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Cheyletus malaccensis releases for the biocontrol of Liposcelis bostrychophila: Density-dependent efficacy in stored grains.","authors":"Yiwang Hu, Dingrong Xue, Panpan Zhang, Zhiqi Liu, Zhiyi Fang, Yu Zhang, Yi Wu","doi":"10.1007/s10493-025-01043-5","DOIUrl":"10.1007/s10493-025-01043-5","url":null,"abstract":"<p><p>Cheyletus malaccensis (Oudemans), a generalist predatory mite, is a highly promising biocontrol agent applied for stored products pests management in grain storage systems. To optimize its field deployment, this study evaluated the population dynamics of C. malaccensis and Liposcelis bostrychophila (Badonnel) under varying predator-prey ratios (PPRs: 1:9, 1:5, 1:3, 1:1, 3:1) at 26.0 ± 0.1 °C and 75.0 ± 0.5% relative humidity in simulated grain storage environments. During the 49-day post-release period, the peak population reduction rates of L. bostrychophila at four PPRs (1:5, 1:3, 1:1, 3:1) reached 74.87%, 76.61%, 87.10%, and 95.90% respectively, whereas the 1:9 PPR treatment resulted in progressive pest population growth. Conversely, C. malaccensis population establishment rates increased exponentially with decreasing PPRs, peaking at 1,161.11% (1:9 PPR). These findings demonstrate a density-dependent trade-off: higher PPRs maximize short-term pest suppression, while lower ratios enhance long-term predator persistence. Therefore, we propose a two-phase deployment strategy: (i) preventive low-density releases (PPR < 1:5) prior to pest outbreaks to establish mite populations, and (ii) threshold-triggered supplementary releases based on real-time pest monitoring to achieve economical and long-term control of stored grain pests.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 2","pages":"19"},"PeriodicalIF":1.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ramification of relaxed thermoregulation by disease vectors under climate change.","authors":"Hugh Lefcort, Sean M Brockman, Jaxon W Hopkins, Sierra M Salter","doi":"10.1007/s10493-025-01041-7","DOIUrl":"10.1007/s10493-025-01041-7","url":null,"abstract":"<p><p>Understanding how vector species such as ticks respond behaviorally to thermal and host-related cues is critical for predicting the effects of climate change on disease transmission. Ticks employ distinct questing strategies-ranging from static sit-and-wait behavior to active host seeking-that influence their exposure to abiotic stressors. We investigated whether Dermacentor andersoni and Dermacentor similis, two sit-and-wait tick species native to Eastern Washington, USA, exhibit behavioral plasticity in response to temperature gradients and host stimuli. We conducted three experiments to assess (1) thermal preferences of D. andersoni under host (dog) scent conditions, (2) thermotactic responses of both species to infrared (IR) radiation in the presence of dog odor, and (3) D. andersoni's approach behavior toward human hosts at various distances, i.e., a host emitting a combination of IR, CO₂, and odors. In thermal gradient assays, D. andersoni showed significantly increased movement toward warmer zones following CO₂ exposure and exhibited wide thermal preferences depending on specific dog odors. However, when exposed to an IR source, we found strong sit-and-wait behaviors by D. andersoni and D. similis regardless of temperature or radiation. The ticks were not attracted to infrared radiation and did not move toward the stationary exposed hand of an observer. Ticks may prioritize optimal locations to encounter potential hosts, over enzymatically optimal temperatures. Rather than evolving to detect hosts at a distance, Haller's organs may have evolved to differentiate warm attachment sites from cooler fur. Our results suggest that Dermacentor questing behavior (remaining on station despite a different preferred temperature) may make them particularly vulnerable to future rises in temperature.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"95 2","pages":"14"},"PeriodicalIF":1.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}