European Journal of Neuroscience最新文献_第9页

Characterising a New Sheep Model of Parkinson's Disease Using Unilateral Intracerebral Injection of 6-Hydroxydopamine Into the Substantia Nigra

IF 2.7 4区医学

European Journal of Neuroscience Pub Date : 2025-01-22 DOI: 10.1111/ejn.16668

Ashik Banstola, Nicolas Vautrelle, David Bergin, Younus Mohammad, Kushan Gandhi, Shakila Rizwan, John N. J. Reynolds

{"title":"Characterising a New Sheep Model of Parkinson's Disease Using Unilateral Intracerebral Injection of 6-Hydroxydopamine Into the Substantia Nigra","authors":"Ashik Banstola, Nicolas Vautrelle, David Bergin, Younus Mohammad, Kushan Gandhi, Shakila Rizwan, John N. J. Reynolds","doi":"10.1111/ejn.16668","DOIUrl":"10.1111/ejn.16668","url":null,"abstract":"<div>\u0000 \u0000 <p>New therapeutic agents developed for treating neurological disorders are often tested successfully on rodents. Testing in an appropriate large animal model where there is longer lifespan and comparable brain size to humans should improve translational success and is frequently expected by regulatory bodies. In this project, we aimed to establish a novel sheep model of Parkinson's disease as a large-brained experimental model for translational research. Our objective was to create a sheep model of Parkinson's disease by unilaterally infusing the neurotoxin 6-hydroxydopamine into the substantia nigra pars compacta. This approach, previously used to induce parkinsonism in rat and non-human primate models, causes dopaminergic imbalance and induces rotational behaviour in quadrupeds challenged with dopaminergic receptor agonists. In the present sheep study, the mixed dopamine receptor agonist apomorphine, 0.25 mg/kg, and dopamine D2 agonist ropinirole, 0.16 mg/kg, were used to induce rotational behaviour and confirm dopamine depletion. Behavioural signs were then measured and characterised in the field using automated movement tracking with simultaneous video recordings. Post-mortem, the extent of the 6-hydroxydopamine lesions was evaluated through tyrosine hydroxylase immunohistochemistry and quantifying levels of catecholamines (dopamine, 3,4-dihydroxyphenylacetic acid and homovanilic acid) quantified using high-performance liquid chromatography. Our new sheep model of Parkinson's disease using 6-hydroxydopamine is safe and offers a number of regulatory, ethical and financial advantages over non-human primate 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine models. It provides a platform to evaluate novel antiparkinsonian agents and medical devices in a large brain with the promise of greater success for translation into clinical application.</p>\u0000 </div>","PeriodicalId":11993,"journal":{"name":"European Journal of Neuroscience","volume":"61 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Rat Model for Inflammatory Gut–Brain Interactions in Parkinson's Disease

IF 2.7 4区医学

European Journal of Neuroscience Pub Date : 2025-01-22 DOI: 10.1111/ejn.16667

Grace E. Kendall, Conor F. Underwood, Louise C. Parr-Brownlie

{"title":"A Novel Rat Model for Inflammatory Gut–Brain Interactions in Parkinson's Disease","authors":"Grace E. Kendall, Conor F. Underwood, Louise C. Parr-Brownlie","doi":"10.1111/ejn.16667","DOIUrl":"10.1111/ejn.16667","url":null,"abstract":"<p>Gut inflammation is a salient prodromal feature of Parkinson's disease (PD) implicated in pathologic processes leading to nigrostriatal dopaminergic degeneration. However, existing rodent models of PD are suboptimal for investigating the interaction between gut inflammation and neuropathology. This study aimed to develop a rat model of PD in which gut inflammation exacerbated PD symptoms induced by a parkinsonian lesion. This was achieved by combining the 6-hydroxydopamine (6-OHDA) rat model for PD and the dextran sodium sulfate (DSS) rat model for colitis. The model was characterised using behavioural tests, including reaching, step, gait, open-field and cylinder tests, plus stereological quantification of substantia nigra (SN) DA neurodegeneration, and histological analysis of SN microglial activation and distal colon morphology. The combination of 6-OHDA and DSS resulted in greater stool softening and bleeding, shorter colons and greater distal colon histological damage, when compared with the 6-OHDA model. Additionally, 6-OHDA and DSS rats displayed similar DA neurodegeneration, yet less SN microglial activation, when compared to 6-OHDA rats that did not receive DSS. Finally, DSS + 6-OHDA rats exhibited impaired forelimb motor function compared with 6-OHDA rats, with decreased performance in reaching and step tests. In conclusion, DSS administration exacerbated forelimb motor dysfunction in 6-OHDA rats. Behavioural changes in DSS + 6-OHDA rats were associated with lower levels of microglial activation and similar levels of dopamine depletion compared with 6-OHDA-only rats. These results support that the DSS + 6-OHDA rat model is a promising PD animal model to investigate deleterious gut–brain interactions in PD.</p>","PeriodicalId":11993,"journal":{"name":"European Journal of Neuroscience","volume":"61 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Severity of Repetitive Mild Traumatic Brain Injury Depends on Microglial Heme Oxygenase-1 and Carbon Monoxide

IF 2.7 4区医学

European Journal of Neuroscience Pub Date : 2025-01-22 DOI: 10.1111/ejn.16666

Sandra Kaiser, Anna Fritsch, Lena Jakob, Nils Schallner

{"title":"Severity of Repetitive Mild Traumatic Brain Injury Depends on Microglial Heme Oxygenase-1 and Carbon Monoxide","authors":"Sandra Kaiser, Anna Fritsch, Lena Jakob, Nils Schallner","doi":"10.1111/ejn.16666","DOIUrl":"10.1111/ejn.16666","url":null,"abstract":"<p>Traumatic brain injury is one of the most common cerebral incidences worldwide. Repetitive mild traumatic brain injuries occurring, for example, in athletes or victims of abuse, can cause chronic neurodegeneration due to neuroinflammation, in which the crosstalk between reactive astrocytes and activated microglia is crucial for modulating neuronal damage. The inducible enzyme heme oxygenase-1 and its product carbon monoxide are known to be ascribed neuroprotective and anti-inflammatory properties. We caused repetitive mild traumatic brain injuries in wild-type mice compared to mice without microglial heme oxygenase-1 expression. Additionally, mice were treated daily with either air or carbon monoxide exogenously. In wild-type mice, we observed enhanced microglia activation and astrogliosis as well as vasodilation after repetitive trauma. In heme oxygenase-1 knockout mice, we observed enhanced activation of microglia and astrocytes at baseline pretrauma with a lack of an adequate inflammatory response to repetitive injury. However, the knockout led to enhanced NF-κB and IFNγ expression in the post-trauma period. Carbon monoxide exerted neuroprotection, as suggested by reduced wake-up times in mice and by beneficially altering inflammation post-traumatic brain injury. This study further underlines the crucial role of the heme oxygenase-1/carbon monoxide system in the modulation of neuronal damage and the associated neuroinflammatory response after repetitive traumatic brain injury.</p>","PeriodicalId":11993,"journal":{"name":"European Journal of Neuroscience","volume":"61 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11755003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Alteration of Brain Network Topology in Tinnitus Transition From Recent-Onset to Chronic 耳鸣由初发向慢性转变时脑网络拓扑结构的改变。

IF 2.7 4区医学

European Journal of Neuroscience Pub Date : 2025-01-21 DOI: 10.1111/ejn.16664

Jiapei Xie, Weidong Zhang, Wei Wei, Yan Bai, Yu Shen, Nan Meng, Xinhui Wang, Meiyun Wang

{"title":"The Alteration of Brain Network Topology in Tinnitus Transition From Recent-Onset to Chronic","authors":"Jiapei Xie, Weidong Zhang, Wei Wei, Yan Bai, Yu Shen, Nan Meng, Xinhui Wang, Meiyun Wang","doi":"10.1111/ejn.16664","DOIUrl":"10.1111/ejn.16664","url":null,"abstract":"<div>\u0000 \u0000 <p>The occurrence and persistence of tinnitus result from the interaction of multiple neural networks. This study aims to explore the alterations in brain network topology associated with the transition of tinnitus from recent-onset to chronic. Twenty-eight patients with chronic tinnitus, 28 patients with recent-onset tinnitus and 28 sex- and age-matched healthy controls (HC) were enrolled in this study. We performed a graph theory analysis to identify aberrant brain network topologies and calculated the correlation between differential brain regions and clinical indicators. Compared with the recent-onset tinnitus group, patients with chronic tinnitus showed decreased global efficiency (Eg, decreased by 3.7%, <i>p</i> < 0.001), local efficiency (Eloc, decreased by 1.8%, <i>p</i> = 0.031) and small-worldness (decreased by 13.8%, <i>p</i> = 0.007) and increased characteristic path length (Lp, increased by 6.8%, <i>p</i> = 0.001). Additionally, ANOVA revealed significant differences in the AUC of degree centrality (DC), nodal efficiency (Ne), nodal clustering coefficient (NCp) and nodal local efficiency (Nle) among the three groups in brain regions such as the superior temporal gyrus, inferior temporal gyrus, anterior cingulate cortex, precuneus, middle occipital gyrus, inferior occipital gyrus, fusiform gyrus, cuneus and putamen (<i>q</i> < 0.05, FDR corrected). Notably, several of these regions were associated with tinnitus duration, distress and loudness. The topological properties of several brain networks were altered in patients with chronic tinnitus compared to those with recent-onset tinnitus, providing new insights into the neural mechanisms of tinnitus chronification. These findings could inform the development of targeted interventions aimed at mitigating the progression from recent-onset to chronic tinnitus.</p>\u0000 </div>","PeriodicalId":11993,"journal":{"name":"European Journal of Neuroscience","volume":"61 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pallidal Spike-Train Variability and Randomness Are the Most Important Signatures to Classify Parkinson's Disease and Cervical Dystonia 苍白突-列变异性和随机性是区分帕金森病和颈肌张力障碍最重要的特征。

IF 2.7 4区医学

European Journal of Neuroscience Pub Date : 2025-01-20 DOI: 10.1111/ejn.16653

A. Sedov, P. Pavlovsky, V. Filyushkina, I. Dzhalagoniya, U. Semenova, N. Zakharov, A. Gamaleya, A. Tomskiy, Aasef G. Shaikh

{"title":"Pallidal Spike-Train Variability and Randomness Are the Most Important Signatures to Classify Parkinson's Disease and Cervical Dystonia","authors":"A. Sedov, P. Pavlovsky, V. Filyushkina, I. Dzhalagoniya, U. Semenova, N. Zakharov, A. Gamaleya, A. Tomskiy, Aasef G. Shaikh","doi":"10.1111/ejn.16653","DOIUrl":"10.1111/ejn.16653","url":null,"abstract":"<div>\u0000 \u0000 <p>Movement disorders such as Parkinson's disease (PD) and cervical dystonia (CD) are associated with abnormal neuronal activity in the globus pallidus internus (GPi). Reduced firing rate and presence of spiking bursts are typical for CD, whereas PD is characterized by high frequency tonic activity. This research aims to identify the most important pallidal spiking parameters to classify these conditions. We analysed the single unit activity of the globus pallidus externus (GPe) and internus (GPi) in 11 CD and 10 PD patients who underwent standard-of-care DBS implantation. We compared firing rate, firing pattern and oscillatory characteristics of tonic, burst and pause cells and used logistic regression and random forest models to classify patients according to their pallidal activity. In the GPi, we discovered prevalence of high firing rate tonic cells in patients with PD, whereas in dystonia, burst neurons with high firing rate were predominant. GPi pause cells were mostly observed in CD patients and exhibited less spike variability compared to PD. Characteristics of neurons and their distribution in the GPe was similar. Logistic regression and random forest models identified spike variability and randomness as the key features for distinguishing between PD and CD, instead of firing rate or oscillation properties. Our study demonstrates that pallidal activity can predict PD and CD with high accuracy. Burst dynamics and characteristics of spiking randomness including entropy appear to be the most meaningful reflections of the neurophysiology of studied diseases.</p>\u0000 </div>","PeriodicalId":11993,"journal":{"name":"European Journal of Neuroscience","volume":"61 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Living in Biological Darkness II: Impact of Winter Habitual Daytime Light on Night-Time Sleep 生活在生物黑暗II：冬季习惯白天光照对夜间睡眠的影响。

IF 2.7 4区医学

European Journal of Neuroscience Pub Date : 2025-01-20 DOI: 10.1111/ejn.16647

Claudia Nowozin, Amely Wahnschaffe, Jan de Zeeuw, Alexandra Papakonstantinou, Sven Hädel, Andrea Rodenbeck, Frederik Bes, Dieter Kunz

{"title":"Living in Biological Darkness II: Impact of Winter Habitual Daytime Light on Night-Time Sleep","authors":"Claudia Nowozin, Amely Wahnschaffe, Jan de Zeeuw, Alexandra Papakonstantinou, Sven Hädel, Andrea Rodenbeck, Frederik Bes, Dieter Kunz","doi":"10.1111/ejn.16647","DOIUrl":"10.1111/ejn.16647","url":null,"abstract":"<p>Timing and architecture of sleep are co-driven by circadian rhythms modulated by their major <i>Zeitgeber</i> light and darkness. In a natural environment, one is exposed to 3.000 lx (cloudy winter sky) to 100.000 lx (bright sunny sky). The aim of the study was to assess (1) habitual daytime light exposure in urban winter and (2) impact of daytime urban light on objective night-time sleep. Eleven healthy participants (mean age ± SD: 25.4 ± 2.8 years; 6 male) wore eyeglass frames continuously recording daytime illuminance levels vertically to the eye by mounted sensors (range: 1–40.000 lx) during four consecutive days in winter 2008 in Berlin, Germany. In-lab polysomnography was performed over two nights in nine participants. Median light exposure over 4 days was the following: full day 7:00–19:00 h: 23 lx (12–37 lx); morning 7:00–11:00 h: 81 lx (19–201 lx); midday 11:00–15:00 h: 68 lx (19–164 lx); afternoon 15:00–19:00 h: 22 lx (6–58 lx), resulting in only 36 min > 500 lx per day. Timing of daytime light intensity was significantly associated with subsequent sleep: lower midday illuminance with shorter REM latency (Rho = 0.817; <i>p</i> = 0.049) and earlier REM polarity (less prevalence of REM at end-of-sleep; Rho = 0.817; <i>p</i> = 0.049). Humans, living in an urban environment, appear to be exposed to extremely low light levels, which we named as ‘<i>Living in Biological Darkness</i>’. Most fascinating, physiology seems to adapt and responds to variation in light intensity on such low levels. Interestingly, the observed changes in sleep architecture with low light levels are reminiscent of those suspected to constitute biological markers of depression some 40–50 years ago.</p>","PeriodicalId":11993,"journal":{"name":"European Journal of Neuroscience","volume":"61 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship Between Structural and Functional Network Connectivity Changes for Patients With Traumatic Brain Injury and Chronic Health Symptoms 外伤性脑损伤患者结构和功能网络连通性变化与慢性健康症状的关系

IF 2.7 4区医学

European Journal of Neuroscience Pub Date : 2025-01-20 DOI: 10.1111/ejn.16678

Xiaojian Kang, Emily Grossner, Byung C. Yoon, Maheen M. Adamson

{"title":"Relationship Between Structural and Functional Network Connectivity Changes for Patients With Traumatic Brain Injury and Chronic Health Symptoms","authors":"Xiaojian Kang, Emily Grossner, Byung C. Yoon, Maheen M. Adamson","doi":"10.1111/ejn.16678","DOIUrl":"10.1111/ejn.16678","url":null,"abstract":"<div>\u0000 \u0000 <p>Combination of structural and functional brain connectivity methods provides a more complete and effective avenue into the investigation of cortical network responses to traumatic brain injury (TBI) and subtle alterations in brain connectivity associated with TBI. Structural connectivity (SC) can be measured using diffusion tensor imaging to evaluate white matter integrity, whereas functional connectivity (FC) can be studied by examining functional correlations within or between functional networks. In this study, the alterations of SC and FC were assessed for TBI patients, with and without chronic symptoms (TBIcs/TBIncs), compared with a healthy control group (CG). The correlation between global SC and FC was significantly increased for both TBI groups compared with CG. SC was significantly lower in the TBIcs group compared with CG, and FC changes were seen in the TBIncs group compared with CG. When comparing TBI groups, FC differences were observed in the TBIcs group compared with the TBIncs group. These observations show that the presence of chronic symptoms is associated with a distinct pattern of SC and FC changes including the atrophy of the SC and a mixture of functional hypoconnectivity and hyperconnectivity, as well as loss of segregation of functional networks.</p>\u0000 </div>","PeriodicalId":11993,"journal":{"name":"European Journal of Neuroscience","volume":"61 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Causal Relationship Between Intestinal Microbiota, Inflammatory Cytokines, Peripheral Immune Cells, Plasma Metabolome and Parkinson's Disease: A Mediation Mendelian Randomization Study 肠道菌群、炎症细胞因子、外周免疫细胞、血浆代谢组与帕金森病的因果关系：一项中介孟德尔随机研究

IF 2.7 4区医学

European Journal of Neuroscience Pub Date : 2025-01-20 DOI: 10.1111/ejn.16665

Chengcheng Wang, Yuhang Tang, Tao Yang, Yuhao Wang, Zihui Niu, Kang Zhang, Ning Lin, Qun Li

{"title":"Causal Relationship Between Intestinal Microbiota, Inflammatory Cytokines, Peripheral Immune Cells, Plasma Metabolome and Parkinson's Disease: A Mediation Mendelian Randomization Study","authors":"Chengcheng Wang, Yuhang Tang, Tao Yang, Yuhao Wang, Zihui Niu, Kang Zhang, Ning Lin, Qun Li","doi":"10.1111/ejn.16665","DOIUrl":"10.1111/ejn.16665","url":null,"abstract":"<div>\u0000 \u0000 <p>Parkinson's disease (PD) is a neurodegenerative disease involving multiple factors. We explored the connection between intestinal microbiome levels and PD by examining inflammatory cytokines, peripheral immune cell counts and plasma metabolomics as potential factors. By obtaining the Genome-Wide Association Study (GWAS) data needed for this study from GWAS Catalog, including summary data for 473 intestinal microbiota traits (<i>N</i> = 5959), 91 inflammatory cytokine traits (<i>N</i> = 14,824), 118 peripheral immune cell count traits (<i>N</i> = 3757), 1400 plasma metabolite traits (<i>N</i> = 8299) and PD traits (<i>N</i> = 482,730). We used two-step Mendelian randomization (MR) mediated analysis to investigate possible pathways from intestinal microbiota to PD mediated by inflammatory cytokines, peripheral immune cells and plasma metabolites. MR has revealed the causal effects of 19 intestinal microbiota, 1 inflammatory cytokine and 12 plasma metabolites on PD, whereas there is no significant causal relationship between immune cell count characteristics and the occurrence of PD. Mediation analysis showed that the associations between the genus Demequina and PD were mediated by tryptophan with mediated proportions of 17.51% (<i>p</i> = 0.0393). Our study demonstrates that genus Demequina may promote the occurrence of PD by reducing the levels of tryptophan.</p>\u0000 </div>","PeriodicalId":11993,"journal":{"name":"European Journal of Neuroscience","volume":"61 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hemispheric Asymmetry of Intracortical Myelin Orientation in the Mouse Auditory Cortex 小鼠听觉皮层内髓鞘定向的半球不对称性。

IF 2.7 4区医学

European Journal of Neuroscience Pub Date : 2025-01-20 DOI: 10.1111/ejn.16675

Philip Ruthig, Gesine Fiona Müller, Marion Fink, Nico Scherf, Markus Morawski, Marc Schönwiesner

{"title":"Hemispheric Asymmetry of Intracortical Myelin Orientation in the Mouse Auditory Cortex","authors":"Philip Ruthig, Gesine Fiona Müller, Marion Fink, Nico Scherf, Markus Morawski, Marc Schönwiesner","doi":"10.1111/ejn.16675","DOIUrl":"10.1111/ejn.16675","url":null,"abstract":"<p>Communication sound processing in mouse AC is lateralized. Both left and right AC are highly specialised and differ in auditory stimulus representation, functional connectivity and field topography. Previous studies have highlighted intracortical functional circuits that explain hemispheric stimulus preference. However, the underlying microstructure remains poorly understood. In this study, we examine structural lateralization of AC on the basis of immunohistochemically stained and tissue-cleared adult mouse brains (<i>n</i> = 11). We found hemispheric asymmetries of intracortical myelination, most prominently in layer 2/3, which featured more intercolumnar connections in the right AC. Furthermore, we found a larger structural asymmetry in the right AC. We also investigated sex differences. In male mice, myelination direction in the right AC is tilted to the anterior side. This pattern is inverted in female mice. However, the spatial distribution of neuronal cell bodies in the left and right AC along the laminar axis of the cortex was remarkably symmetric in all samples. These results suggest that basic developmentally defined structures such as cortical columns remain untouched by lateral specialisation, but more plastic myelinated axons show diverse hemispheric asymmetries. These asymmetries may contribute to specialisation on lateralized tasks such as vocal communication processing or specialisation on spectral or temporal complexity of stimuli.</p>","PeriodicalId":11993,"journal":{"name":"European Journal of Neuroscience","volume":"61 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postcolitis Alterations in Dose-Dependent Effects of 5-HT1A Agonist Buspirone on Nociceptive Activity of the Raphe Magnus and Dorsal Raphe Neurons in Rats 5-HT1A激动剂丁螺环酮对大鼠中缝大鼠和中缝背神经元伤害性活动的剂量依赖性影响。

IF 2.7 4区医学

European Journal of Neuroscience Pub Date : 2025-01-20 DOI: 10.1111/ejn.16677

Olga A. Lyubashina, Boris M. Sushkevich, Ivan B. Sivachenko

{"title":"Postcolitis Alterations in Dose-Dependent Effects of 5-HT1A Agonist Buspirone on Nociceptive Activity of the Raphe Magnus and Dorsal Raphe Neurons in Rats","authors":"Olga A. Lyubashina, Boris M. Sushkevich, Ivan B. Sivachenko","doi":"10.1111/ejn.16677","DOIUrl":"10.1111/ejn.16677","url":null,"abstract":"<div>\u0000 \u0000 <p>The serotonergic raphe magnus (RMg) and dorsal raphe (DR) nuclei are crucial pain–regulating structures, which nociceptive activity is shown to be altered in gut pathology, but the underlying neuroplastic changes remain unclear. Considering the importance of 5-HT1A receptors in modulating both pain and raphe neuronal activity, in this study, we aimed to determine whether 5-HT1A-dependent visceral and somatic nociceptive processing within the RMg and DR is modified in postcolitis conditions. In anaesthetised male Wistar rats, healthy control and recovered from TNBS-induced colitis, the microelectrode recordings of RMg and DR neuron responses to noxious colorectal distension (CRD) or tail squeezing (TS) were performed prior and after intravenous administration of 5-HT1A agonist, buspirone. In postcolitis animals, 5-HT1A autoreceptor- and heteroreceptor-activating high doses of buspirone (2 and 4 mg/kg) lost normally occurring ability to facilitate CRD- and TS-evoked activation of RMg neurons, causing inhibition of the local nociceptive signalling similar to 5-HT1A autoreceptor-activating low doses (0.1 and 0.5 mg/kg). Conversely, the normally inherent property of buspirone at all doses to reduce visceral and somatic pain–related neuronal excitation in the DR was weakened after colitis. These phenomena were associated with a loss of normally occurring inhibitory effect of the compound's high doses on hemodynamic reactions to CRD and TS, revealing deficient antinociceptive action at a systemic level. The data suggest postcolitis changes in buspirone-dependent 5-HT1A autoreceptor- and heteroreceptor-mediated signalling, which can directly or indirectly lead to reduced RMg pain–related activity and increased DR nociceptive excitation, impairing their functioning in the visceral and somatic pain control.</p>\u0000 </div>","PeriodicalId":11993,"journal":{"name":"European Journal of Neuroscience","volume":"61 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0