{"title":"Influence of rubbing during etching on surface roughness and interfacial characteristics of intact enamel.","authors":"Moana Biedermann, Iosif Sifakakis, Spiros Zinelis, George Eliades, Theodore Eliades, Raphael Patcas","doi":"10.1111/eos.70108","DOIUrl":"https://doi.org/10.1111/eos.70108","url":null,"abstract":"<p><p>Rubbing phosphoric acid during etching has been shown to improve surface roughness in prepared enamel. This study aimed to compare intact enamel's surface roughness and resin tag lengths after etching with and without rubbing. Extracted sound third molars were used for testing, mesially for conventional and distally for dynamic (rubbing) etchant application. All surfaces were etched for 20 s with 37% phosphoric acid etchant and rinsed with water for 10 s. Optical profilometry and high-vacuum scanning electron microscopy (HV-SEM) were used for enamel surface assessment and evaluation of the infiltrating capacity of a bonding resin into enamel. Statistical analyses were done by a paired t-test. The obtained results demonstrated that rubbing the acid agent during etching showed no statistically significant increase in enamel surface roughness. In both groups, texture aspect ratio (Str) values were close to 1 (standard Str = 0.86, rubbing Str = 0.90), indicating that both surfaces can be considered isotropic. HV-SEM images showed a more typical etching pattern after rubbing, but with insignificant differences in the height of the resin tags. It can be concluded that rubbing during the etching of intact enamel results in a similar surface roughness and resin-enamel interface compared with the conventional etching technique. This stands in contrast to the reported improvement achieved on prepared enamel.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":" ","pages":"e70108"},"PeriodicalIF":1.8,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147981082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hanemi Tsuruta, Shojiro Shimizu, Norihiro Sasamoto, Richard M Foxton, Tomofumi Sawada, Toru Nikaido, Katsushi Okuyama
{"title":"Evaluation of the impact of toothbrush wear on artificial plaque removal efficacy.","authors":"Hanemi Tsuruta, Shojiro Shimizu, Norihiro Sasamoto, Richard M Foxton, Tomofumi Sawada, Toru Nikaido, Katsushi Okuyama","doi":"10.1111/eos.70109","DOIUrl":"https://doi.org/10.1111/eos.70109","url":null,"abstract":"<p><p>This study evaluated the artificial plaque removal efficacy and wear patterns of three high-density, ultrasoft toothbrushes with polyester filaments (standard, small, and large head sizes) after simulated long-term use. A 3D-printed jaw model and artificial teeth were used, and brushing tests corresponding to 3 months (3 M) and 6 months (6 M) of use were performed. The wear index (WI) was calculated, and morphological changes of the filament tips were examined using a digital microscope. The artificial plaque retention rate was then measured for new brushes (Control), 3 M, and 6 M specimens. In the small-head toothbrush, the artificial plaque retention rate significantly increased after 6 M compared with the control and 3 M. In contrast, no significant differences were observed across usage periods in the standard- and large-head toothbrushes. The WI increased over time in all toothbrushes, with significantly higher values at 3 M and 6 M in the small-head group. Flattening of the filament tips was observed with increasing simulated use. These findings suggest that high-density toothbrushes with an appropriate head size can maintain artificial plaque removal efficacy for up to 3 months, whereas head size influences cleaning performance and wear characteristics after prolonged use.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":" ","pages":"e70109"},"PeriodicalIF":1.8,"publicationDate":"2026-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147971547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Response to Letter to the Editor","authors":"Line Staun Larsen","doi":"10.1111/eos.70067","DOIUrl":"10.1111/eos.70067","url":null,"abstract":"","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145818593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gisela Rodrigues da Silva Sasso, Estela Sasso-Cerri, Paulo Sérgio Cerri, Manuel de Jesus Simões, Rinaldo Florencio-Silva
{"title":"Interplay between autophagy and NLRP3 inflammasome in the gingival mucosa of estrogen-deficient and streptozotocin-induced diabetic rats","authors":"Gisela Rodrigues da Silva Sasso, Estela Sasso-Cerri, Paulo Sérgio Cerri, Manuel de Jesus Simões, Rinaldo Florencio-Silva","doi":"10.1111/eos.70064","DOIUrl":"10.1111/eos.70064","url":null,"abstract":"<p>This study investigated how estrogen deficiency and streptozotocin-induced diabetes mellitus (DM) affect rat gingival mucosa, focusing on autophagy and NLRP3 inflammasome expression. Twenty rats were ovariectomized (OVX) or SHAM-operated (SHAM) and divided into SHAM, OVX, SHAM-DM, and OVX-DM groups. After 7 weeks, rats were euthanized, and maxillae containing gingival mucosa were processed for histology and immunohistochemistry for autophagy (Beclin-1, LC3B) and inflammasome (NLRP3, IL-1β) markers. Publicly available data were analyzed to assess these markers in gingival tissue from periodontitis patients. Epithelial and stratum corneum thickness increased in OVX, SHAM-DM, and OVX-DM groups. SHAM-DM and OVX-DM rats showed more keratinocytes with large vesicular nuclei and prominent nucleoli. The highest numbers of mast cells, especially degranulated ones, occurred in OVX-DM. Increased Beclin-1, LC3B, NLRP3, and IL-1β expression was detected in the lamina propria and gingival epithelium, particularly in OVX-DM. These markers showed positive correlations. Bioinformatics revealed increased LC3B, NLRP3, and IL1B transcription in periodontitis patients’ gingiva. Positive correlations were found between <i>BECN1</i> and <i>LC3B</i>, <i>BECN1</i> and <i>NLRP3</i>, <i>LC3B</i> and <i>NLRP3</i>, and <i>NLRP3</i> and <i>IL1B</i>; <i>BECN1</i> and <i>IL1B</i> correlated negatively. These findings suggest that increased autophagy and NLRP3 inflammasome activation contribute to gingival mucosa damage in estrogen-deficient diabetic rats and in periodontitis patients.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145911005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}