European Journal of Medical Research最新文献

{"title":"Assessing hemoglobin, albumin, lymphocyte, platelet (HALP) score in predicting strangulation and resection in hernia surgery.","authors":"Seyit Murat Aydın, İsmail Hakkı Şencan, Muhammed Kerem Deli, Ayşe Nur Kartal, Tayfun Bayraktar, Yunushan Furkan Aydoğdu, Aziz Mutlu Barlas","doi":"10.1186/s40001-025-03185-z","DOIUrl":"10.1186/s40001-025-03185-z","url":null,"abstract":"<p><strong>Background: </strong>Incarcerated hernias carry a risk of strangulation, which can lead to bowel ischemia, necrosis, and the need for organ resection. The Hemoglobin, Albumin, Lymphocyte, Platelet (HALP) score, a composite biomarker of nutritional and inflammatory status, has shown prognostic value in various malignant and inflammatory conditions. This study aimed to evaluate the role of HALP score in predicting strangulation and the need for bowel or tissue resection in patients undergoing surgery for incarcerated abdominal wall hernias.</p><p><strong>Methods: </strong>We retrospectively analyzed the data of 135 patients who underwent elective or emergency hernia surgery due to incarceration between 2015 and January 2025. Patients were divided into two groups: those requiring tissue or organ resection due to intraoperative signs of strangulation (n = 53) and those who underwent hernia repair without resection (n = 82). HALP scores were calculated using preoperative laboratory values. Statistical analyses were performed using non-parametric tests due to the non-normal distribution of variables. A p-value < 0.05 was considered statistically significant.</p><p><strong>Results: </strong>Of the 135 patients included, 53 (39.3%) underwent bowel or tissue resection due to intraoperative evidence of strangulation, while 82 (60.7%) underwent hernia repair without resection. Patients in the resection group tended to be older (median age: 72 vs. 64 years, p = 0.083). The median HALP score was notably lower in the strangulation group (2.55) compared to the non-resection group (4.55), though the difference did not reach statistical significance (p = 0.092). The most frequently resected tissues were small intestine (41.5%) and omentum (35.8%). Resection was significantly associated with the presence of ischemia (p < 0.001), whereas no significant associations were found with hernia type, side, or surgical approach.</p><p><strong>Conclusion: </strong>A decreasing trend was observed, the trend toward lower HALP scores in patients with strangulated hernias suggests a potential role for HALP as a supportive biomarker in preoperative risk assessment. Its low cost and accessibility make it a practical tool, especially in emergency settings. Larger, prospective studies with standardized preoperative protocols are warranted to validate the clinical utility of HALP in predicting ischemic complications in incarcerated hernias.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"895"},"PeriodicalIF":3.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of long non-coding RNAs in cartilage development and osteoarthritis pathogenesis.","authors":"Xuejiao Sun, Shan Wang, Jun Tian, Mingxing Li","doi":"10.1186/s40001-025-03173-3","DOIUrl":"10.1186/s40001-025-03173-3","url":null,"abstract":"<p><p>Osteoarthritis (OA) is a progressive joint disease characterized by the degradation of cartilage, remodeling of subchondral bone, and inflammation of synovial tissue, posing growing global health challenges. Long non-coding RNAs (lncRNAs) play crucial roles in various physiological and pathological processes associated with OA, including extracellular matrix (ECM) degradation, synovial inflammation, chondrocyte apoptosis, and angiogenesis. Chondrogenesis involves mesenchymal cell condensation followed by differentiation into chondrocytes, hypertrophy of chondrocytes, and subsequent maturation. This review aims to provide an updated overview of lncRNAs and their involvement in cartilage development as well as OA. Furthermore, we discuss the potential utility of lncRNAs as molecular biomarkers for early diagnosis and treatment of OA.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"900"},"PeriodicalIF":3.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution effect of SOST on alveolar bone micro-bone formation in rats induced by Eldecalcitol.","authors":"Ying Guo, Hong Wang, Nu Mi, Qianmin Chen, RongRong Wang, Hui Ni","doi":"10.1186/s40001-025-03182-2","DOIUrl":"10.1186/s40001-025-03182-2","url":null,"abstract":"<p><strong>Objectives: </strong>This study controlled experimental and histological study based on an ovariectomized (OVX) rat model, and aimed to clarify the distribution changes of TRAP-positive osteoclasts and sclerostin-positive osteocytes in the alveolar septal area of rats after Eldecalcitol (ELD) treatment, and to explore their association with bone metabolism-related factors, thereby preliminarily elucidating the early pathological mechanism of alveolar osteoporosis.</p><p><strong>Methods: </strong>A total of 32 female Wistar rats (8 weeks) were randomly divided into three groups: sham-operated control (Sham, n = 10), ovariectomized (OVX, n = 11), and ovariectomized treated with Eldecalcitol (OVX + ELD, n = 11). Observations were Made at different timepoints, including 1 week and 2 weeks after surgery. OVX rat models were established by bilateral ovariectomy. Eldecalcitol (0.03 µg/kg/day) was administered orally for 2 weeks starting immediately after surgery. Mandibular samples were fixed, decalcified, and prepared into thin sections for histological analyses. Osteocyte distribution and characteristics were studied in detail using TRAP staining, ALP/TRAP staining, and sclerostin/TRAP/silver triple staining techniques. In addition, double staining of RANKL and TRAP was performed to further explore the expression of bone resorption-related molecules.</p><p><strong>Results: </strong>The experimental results showed that in the bone groove interval area, rats that underwent ovariectomy (OVX) had a small number of TRAP-positive osteoclasts in the early stage (1 week), but this number gradually increased over time. In the OVX group after 1 week of combined treatment with Eldecalcitol, the number of TRAP-positive osteoclasts was significantly reduced. In addition, the expression of sclerostin-positive osteocytes decreased over time, but this decrease was smaller in the combination treatment group.</p><p><strong>Conclusions: </strong>This study reveals the distribution of TRAP-positive osteoclasts and sclerostin-positive osteocytes in the alveolar septal area and their correlation with bone metabolism-related factors. Eldecalcitol treatment effectively inhibits osteoclast activity, reduces bone resorption, and significantly mitigates the reduction of sclerostin expression in osteocytes induced by ovariectomy. These findings suggest that Eldecalcitol may regulate alveolar bone metabolism partially by modulating sclerostin expression, making it a promising therapeutic strategy for osteoporosis.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"886"},"PeriodicalIF":3.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12481801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of core temperature monitoring methods during cytoreductive surgery with hyperthermic intraperitoneal chemotherapy: a prospective observational study.","authors":"Jung-Won Kim, Jin Ho Kim, Soojeong Oh, So Woon Ahn","doi":"10.1186/s40001-025-03207-w","DOIUrl":"10.1186/s40001-025-03207-w","url":null,"abstract":"<p><strong>Background: </strong>During cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy (HIPEC), precise core temperature monitoring is critical for patient safety. This prospective study evaluated the agreement among three core temperature monitoring modalities: nasopharyngeal temperature (Tnaso), zero-heat flux cutaneous thermometer (TSpotOn), and oesophageal temperature (Teso).</p><p><strong>Methods: </strong>Temperatures were measured simultaneously; agreement between monitoring sites was assessed using Bland-Altman analysis for repeated measures including mean bias, 95% limits of agreement, and confidence intervals, alongside Lin's concordance correlation coefficient (LCCC). The proportion of paired differences within the clinically acceptable limit of 0.5 °C was reported.</p><p><strong>Results: </strong>The mean difference between Tnaso and TSpotOn was -0.04 ± 0.33 °C (95% limits: -0.69 to 0.62), and between Tnaso and Teso was 0.02 ± 0.35 °C (95% limits: -0.66 to 0.71), with LCCC for both comparisons at 0.94 (95% CI: 0.93-0.94), indicating substantial agreement. However, up to 14% of Teso pairs exceeded the 0.5 °C threshold, suggesting potential clinical relevance. Notable rapid temperature decline and subsequent rebound were observed post-HIPEC at Tnaso and TSpotOn sites.</p><p><strong>Conclusion: </strong>All three monitoring methods correlated strongly overall, but Teso exhibited phase-dependent discrepancies, particularly after HIPEC. These findings support tailoring core temperature measurement site selection with attention to the target organ, especially in laparotomy-based HIPEC procedures.</p><p><strong>Trial registration: </strong>The study was registered in the clinical trials registry cris.nih.go.kr (Registration Number: KCT0003980).</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"908"},"PeriodicalIF":3.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12481973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine sutures combined with local flaps in the cosmetic repair of surgical defects of basal cell carcinoma of the head and face: a single-centre retrospective clinical study.","authors":"Hongyun Chen, Qiang Li, Nana Zhe, Nanqu Huang","doi":"10.1186/s40001-025-03163-5","DOIUrl":"10.1186/s40001-025-03163-5","url":null,"abstract":"<p><strong>Objective: </strong>To explore the effect of fine suturing combined with local flaps in the cosmetic repair of surgical defects associated with basal cell carcinoma (BCC) of the head and face.</p><p><strong>Methods: </strong>A total of 46 patients with basal cell carcinoma of the head and face who were admitted to Third Affiliated Hospital of Zunyi Medical University (The First People's Hospital of Zunyi) from January 2018 to June 2024 were retrospectively analysed. The patients were divided into a combined group and a control group, with 23 patients in each group. Each patient underwent extended tumour resection combined with intraoperative frozen section examination to ensure negative tumour boundaries. In the combined group, fine suturing and local flap transplantation were used to repair the defects; in the control group, traditional skin grafting was used to repair the defects. The perioperative indicators, postoperative scars, and patient satisfaction were compared between the two groups.</p><p><strong>Results: </strong>The perioperative indicators of operation time and healing time were shorter than those of the control group (P < 0.05), and the incidence of adverse reactions was lower than that of the control group (P < 0.05). The Vancouver scar scale (VSS) score of the combined group was lower than that of the control group after follow-up for 6 months to 2 years, and patient satisfaction was higher (P < 0.05).</p><p><strong>Conclusion: </strong>Fine suturing combined with local flaps manifests a significant cosmetic effect in the repair of surgical defects of BCC of the head and face, which can reduce scar formation and improve patient satisfaction.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"896"},"PeriodicalIF":3.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EMSY enhances glycolysis in ovarian cancer cells.","authors":"Shaosheng Wang, Na Xie, Ping Zha, Yingming Li, Minghui Wei, Ji Bai, Xiaohong Zhao","doi":"10.1186/s40001-025-03133-x","DOIUrl":"10.1186/s40001-025-03133-x","url":null,"abstract":"<p><p>EMSY is critical in the progression of ovarian cancer; nevertheless, its impact on tumor metabolism is not widely known. This study showed ovarian cancer cells overexpressing EMSY produce more lactate and have upregulated LDHA expression; the promoting effect of EMSY on the malignant phenotype of these cells is eliminated when LDHA expression is knocked down or glycolysis is inhibited in ovarian cancer cells. Mechanistic studies revealed that EMSY interacts with β-catenin, and the knockdown of EMSY inhibits the transcriptional activation of LDHA by β-catenin. In summary, this study identifies EMSY's metabolic regulatory role in ovarian cancer cells and offers treatment of ovarian cancer with a new therapeutic target.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"890"},"PeriodicalIF":3.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GDF15 attenuates myocardial infarction-induced injury by preserving mitochondrial function and suppressing oxidative stress.","authors":"Xiaogang Yuan, Cheng Wang, Haiyan Zhu","doi":"10.1186/s40001-025-03144-8","DOIUrl":"10.1186/s40001-025-03144-8","url":null,"abstract":"<p><p>Myocardial infarction, a serious cardiovascular disease, is still a major cause of morbidity and mortality worldwide. Growth differentiation factor-15, a stress-responsive cytokine, has been involved in cardiac pathophysiology, but its exact role in myocardial infarction remains controversial. This study aimed to clarify the mechanisms underlying the cardioprotective effects of GDF-15 in myocardial infarction. By using a combination of in vivo and in vitro methods, including immunofluorescence staining, echocardiography, RNA sequencing, and high-resolution respirometry, we showed that GDF-15 expression is significantly upregulated in infarcted myocardium and its deficiency aggravates cardiac injury. Mechanistically, GDF-15 deficiency impairs mitochondrial function and energy metabolism under hypoxic stress, as evidenced by changes in mitochondrial membrane potential and respiratory parameters. Moreover, we identified that GDF-15 suppresses hypoxia-induced reactive oxygen species generation through activation of the AMPK signaling pathway. Therapeutic administration of exogenous GDF-15 reduces myocardial injury, hypoxic stress, and fibrosis after myocardial infarction, suggesting its potential as a therapeutic target. These findings collectively demonstrate that GDF-15 plays a crucial role in cardiac protection during myocardial infarction by regulating mitochondrial function, energy metabolism, and oxidative stress. Our results provide novel insights into the molecular mechanisms of GDF-15-mediated cardioprotection and suggest its potential as a therapeutic intervention for myocardial infarction. Future studies should focus on translational research to evaluate the clinical efficacy of GDF-15-based therapies in myocardial infarction patients.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"903"},"PeriodicalIF":3.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dysphagia linked to clinical phenotype and disease progression in spinocerebellar ataxia type 3.","authors":"Cai-Ping Chen, Mao-Lin Cui, Wei Lin, Zhuo-Ying Huang, Bei-Ning Ye, Bin Cai, Shi-Rui Gan","doi":"10.1186/s40001-025-03154-6","DOIUrl":"10.1186/s40001-025-03154-6","url":null,"abstract":"<p><strong>Background: </strong>Spinocerebellar Ataxia type 3 (SCA3) is a widely recognized autosomal dominant disorder characterized by cerebellar ataxia, particularly prevalent in China. Dysphagia frequently arises in SCA3 and other neurological disorders, representing a significant threat to patient survival.</p><p><strong>Objective: </strong>Examining the Prevalence of Dysphagia among SCA3 Patients and Its correlation with Clinical phenotype and Disease Progression.</p><p><strong>Methods: </strong>We retrospectively analyzed 183 SCA3 patients, divided into dysphagia and non-dysphagia groups. Dysphagia, as an item within INAS, was assessed by ataxia specialists primarily based on patient-reported symptoms, supplemented by caregiver or family input when available. Spearman's rho tested factor associations with dysphagia, logistic regression identified dysphagia risk factors, and multivariable linear regression assessed dysphagia's effect on ataxia severity. Kaplan-Meier curves with first derivative fitting explored dysphagia progression over the disease duration.</p><p><strong>Results: </strong>The study found 77.0% of SCA3 patients had dysphagia, with disease duration most strongly linked to its onset (r = 0.456, p < 0.001). Sex (p = 0.001; OR = 4.69, 95% CI = 1.85 to 11.88), AAO (p = 0.031; OR = 0.93, 95% CI = 0.87 to 0.99), SARA scores (p = 0.034; OR = 1.12, 95% CI = 1.01 to 1.25), and disease duration (p < 0.001; OR = 1.34, 95% CI = 1.14 to 1.57) were independent dysphagia risk factors. Dysphagia also affected SARA scores (p = 0.048). Dysphagia progression rate peaks within the first decade of disease onset, reaching maximal velocity at 6.5 years, with a median time to dysphagia onset of 9 years.</p><p><strong>Conclusion: </strong>In China, dysphagia frequently occurs in SCA3 patients and can impact the severity of ataxia. The prevalence of dysphagia varies as the disease advances. These findings highlight the importance of timely intervention for dysphagia in SCA3 patients, particularly during the late stages of the first decade.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"901"},"PeriodicalIF":3.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145190988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deferoxamine addresses metabolic dysregulation and urinary tract infections in weight-associated gestational diabetes mellitus.","authors":"Min Zhang, Na Wei, Rong Lin, Yue Xu, Qingfu Zhang, Lina Jia, Xiaotong Zhang, Xiaojing Yang","doi":"10.1186/s40001-025-03013-4","DOIUrl":"10.1186/s40001-025-03013-4","url":null,"abstract":"<p><strong>Background: </strong>The increasing prevalence of gestational diabetes mellitus (GDM), particularly among overweight or obese individuals, poses significant health risks. Excess iron contributes to oxidative stress, inflammation, and disruptions in immune and metabolic functions in GDM. Deferoxamine (DFO), an iron chelator, may offer a therapeutic solution by restoring immune and metabolic balance.</p><p><strong>Methods: </strong>We conducted a comprehensive multi-omics analysis using GEO transcriptomic data, applying Weighted Gene Co-expression Network Analysis (WGCNA) and gene set enrichment analysis to identify key immune-metabolic genes. Molecular docking experiments with DFO were performed using AutoDock Vina, and interactions were visualized in PyMOL. Various in vitro assays-CCK-8, qRT-PCR, Western blot, immunofluorescence, ELISA, and colony formation tests-were conducted under high glucose conditions to assess the effects of DFO, focusing on LAMA3 and the PI3K/AKT signaling pathway.</p><p><strong>Results: </strong>Key genes such as CDR2L, LIMCH1, LDLR, and LAMA3 were identified as being improperly regulated in cases of gestational diabetes mellitus (GDM). DFO demonstrated a significant affinity for these targets, especially LDLR. Functionally, DFO was found to improve cell survival during hyperglycemic stress, mitigate oxidative stress, and lower the concentrations of inflammatory cytokines like IL-6, IL-17, and IL-23. Notably, silencing or blocking LAMA3 reversed these effects, inhibiting the PI3K/AKT pathway, increasing apoptosis markers, and decreasing cell proliferation.</p><p><strong>Conclusions: </strong>DFO holds potential as a targeted treatment for GDM associated with obesity by addressing iron excess and immune-metabolic dysregulation. LAMA3 plays a crucial role in mediating DFO's anti-inflammatory and survival-promoting effects via the PI3K/AKT pathway. Further clinical studies are needed to explore DFO's therapeutic potential in GDM.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"897"},"PeriodicalIF":3.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of intestinal barrier function and microbial diversity changes in L-arginine-induced acute pancreatitis mice.","authors":"Peng Zhang, Yufu Li, Zhenyu Chen, Xinxin Wang, Korotkova Irina Pavlovna","doi":"10.1186/s40001-025-03199-7","DOIUrl":"10.1186/s40001-025-03199-7","url":null,"abstract":"<p><strong>Introduction: </strong>While it has been established that intestinal health and microbial variations can influence pancreatic well-being, the extent of intestinal damage and the composition and diversity of the microbiota in animal models of L-arginine-induced acute pancreatitis (AP) remain unclear.</p><p><strong>Methods: </strong>This study aims to evaluate the extent of intestinal damage and microbial changes in AP mouse models. The male C57BL/6 mice were randomly divided into two groups: the AP group received an intraperitoneal injection of 10% L-arginine, while the control group received an equivalent volume of saline. Histological analysis was used to assess morphological changes and damage in the pancreas and the duodenum, ileum, and jejunum. Fluorescent immunohistochemistry and immunohistochemical techniques were employed to evaluate the expression of myeloperoxidase (MPO) in the pancreas and Zonula occludens-1 (ZO-1) and Occludin in the small intestine. High-throughput sequencing was utilized to analyze the composition and diversity of the intestinal microbiota.</p><p><strong>Results: </strong>L-arginine-induced AP in mice significantly increased serum lipase activity and decreased calcium levels, with an increase in pancreatic MPO expression. Additionally, we observed varying degrees of intestinal villi necrosis, edema, and inflammatory cell infiltration in the duodenum, jejunum, and ileum of the AP group mice. Notably, the expression of epithelial Occludin and ZO-1 was reduced in these regions. Compared to healthy mice, AP mice exhibited reduced bacterial richness and diversity, with changes in the intestinal microbiota including increased prevalence of Bacteroidetes and Proteobacteria, while the abundance of Firmicutes decreased.</p><p><strong>Discussion: </strong>L-arginine-induced acute pancreatitis in mice resulted in significant alterations in the intestinal barrier and microbiota.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"912"},"PeriodicalIF":3.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}