European Journal of Medical Research最新文献

{"title":"Overexpression of miR-192 in fibroblasts accelerates wound healing in diabetic rats: research article.","authors":"Forouzan Karam, Mahtab Sayadi, Saeedeh Dadi, Gholamreza Anani Sarab","doi":"10.1186/s40001-025-02449-y","DOIUrl":"10.1186/s40001-025-02449-y","url":null,"abstract":"<p><strong>Background: </strong>Diabetic foot ulcer (DFU) is a severe diabetic complication. Transplantation of skin substitutes, stem cells, and platelet-rich plasma (PRP) treatments are promising tools to promote ulcer healing in diabetes. An important aspect of the remodeling phase of wound healing is collagen deposition. miR-192 increases the expression of COL1A2 by specifically targeting Smad-interacting protein 1 (SIP1). This study was designed to investigate the impact of combined treatment with platelet-rich plasma and fibroblast cells expressing miR-192 on the healing process of wounds using an experimental diabetic animal model.</p><p><strong>Methods: </strong>After transfection of HDF cells and induction of increased miR-192 expression, relative changes in COL1A2 gene expression were determined by the RT-PCR method. Rats were randomly divided into 6 groups: non-diabetic control group, diabetic control, backbone, PRP, miR-192, and PRP + miR-192 groups. Diabetes was induced in male Wistar rats of all treated groups except non-diabetic control through a 21-day high-fat diet and an intraperitoneal injection of 40 mg/kg streptozotocin. A 10-mm skin biopsy punch was used to create two full-thickness wounds on the dorsal part of the upper body in all six groups of animals. Hematoxylin-eosin and Mason's trichrome staining were used to evaluate the wounds and analyze histological changes.</p><p><strong>Results: </strong>The overexpression of miR-192 in HDF cells resulted in a significant increase in COL1A2 gene expression, which was 15.77-fold higher than the control group. PRP and pLenti-III-miR-192-GFP-expressing cells significantly increased wound closure rates, granulation tissue area, and collagen fiber density in rats, according to a histological examination.</p><p><strong>Conclusion: </strong>The combined use of PRP and HDFs expressing pLenti-III-miR-192-GFP speeds up the healing of wounds by increasing collagen expression, demonstrating the efficacy of this approach in improving wound healing results.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"239"},"PeriodicalIF":2.8,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PD-1 suppression enhances HIV reactivation and T-cell immunity via MAPK/NF-κB signaling.","authors":"Xueru Lin, Bo Song, Lijun Cao, Lin Zhang, Siyu Liu, Xue Wang, Xiaohong Chen, Shuchen Li","doi":"10.1186/s40001-025-02427-4","DOIUrl":"10.1186/s40001-025-02427-4","url":null,"abstract":"<p><strong>Background: </strong>Programmed cell death protein 1 (PD-1) is a key immune checkpoint involved in HIV-related immune escape, but its precise role and underlying mechanisms remain unclear. This study investigates the effects of PD-1 inhibition on HIV infection and T-cell function, focusing on the MAPK and NF-κB signaling pathways.</p><p><strong>Methods: </strong>Peripheral blood mononuclear cells (PBMCs) were isolated from HIV-infected individuals and healthy controls. T-cell subsets were analyzed for PD-1 expression via flow cytometry. The impact of antiretroviral therapy (ART) on T-cell numbers, apoptosis, and PD-1 expression was assessed. PD-1 blockade was performed using pembrolizumab, and its effects on T-cell survival and cytokine secretion were evaluated. MAPK/NF-κB signaling was analyzed using Western blot and co-immunoprecipitation, while latent HIV activation was assessed by measuring HIV-1 LTR transcriptional activity in J-Lat cells. Reverse-ChIP assays explored the interaction between HIV-1 Nef protein and the PD-1 promoter.</p><p><strong>Results: </strong>PD-1 expression was higher in T cells from HIV-infected individuals compared to healthy controls, with no significant change following ART. PD-1 blockade with pembrolizumab reduced T-cell apoptosis and enhanced cytokine secretion (TNF-α, IFN-γ, IL-2). PD-1 inhibition also activated latent HIV in J-Lat cells. Western blotting revealed reduced phosphorylation of MAPK and NF-κB pathway components (p-MEK1/2, p-p38 MAPK, p-NF-κB p65), and co-immunoprecipitation confirmed a direct interaction between PD-1 and SHP-2, regulating these pathways.</p><p><strong>Conclusions: </strong>PD-1 mediates HIV immune evasion through the MAPK/NF-κB pathways. PD-1 blockade restores T-cell function and activates latent HIV, suggesting potential therapeutic strategies for HIV treatment.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"237"},"PeriodicalIF":2.8,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dexmedetomidine for modified electroconvulsive therapy: a dose-optimized treatment study.","authors":"Jun Shen, Min Zhou, Guangliang Zhu, Yu Zhang, Jinzhi Ma, Dekui Li, Lei Chen, Kejun Qi, Anjiang Wang, Yang Jiang, Zhiming Dai, Xiaoming Li","doi":"10.1186/s40001-025-02509-3","DOIUrl":"10.1186/s40001-025-02509-3","url":null,"abstract":"<p><strong>Objectives: </strong>To determine the optimal dexmedetomidine dose for hemodynamic stability and recovery quality in modified electroconvulsive therapy (MECT).</p><p><strong>Methods: </strong>In this randomized trial, 252 patients receiving MECT were allocated to six groups (placebo, D1-D5; 42/group). Groups D1-D5 received dexmedetomidine (0.2-1.0 μg/kg) 10 min pre-anesthesia, while controls received saline. Hemodynamic parameters heart rate (HR), mean arterial pressure (MAP), seizure duration, propofol requirements, recovery times, and adverse events were analyzed.</p><p><strong>Results: </strong>Doses ≥ 0.4 μg/kg (D2-D5) significantly reduced HR and MAP versus control (P < 0.05), with prolonged recovery in D4-D5 (P < 0.05). Seizure duration remained unchanged across groups. Propofol use decreased dose-dependently (D2-D5, P < 0.05). The D2 group (0.4 μg/kg) achieved optimal hemodynamic stability without excessive recovery delays.</p><p><strong>Conclusions: </strong>Dexmedetomidine pretreatment at 0.4 μg/kg optimizes MECT anesthesia by balancing hemodynamic control, reduced propofol use, and rapid recovery, establishing it as the recommended dose.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"241"},"PeriodicalIF":2.8,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of environmental factors on respiratory tract microbiome and respiratory system diseases.","authors":"Yutao Ge, Guo Tang, Yawen Fu, Peng Deng, Rong Yao","doi":"10.1186/s40001-025-02517-3","DOIUrl":"10.1186/s40001-025-02517-3","url":null,"abstract":"<p><p>The respiratory tract microbiome, a complex ecosystem of microorganisms colonizing the respiratory mucous layers and epithelial surfaces along with their associated microenvironment, plays a vital role in maintaining respiratory function and promoting the maturation of the respiratory immune system. Current research suggests that environmental changes can disrupt the respiratory microbiota, potentially leading to disease. This review summarizes existing research on the impact of environmental factors on the respiratory microbiome and associated diseases, aiming to offer new insights into the prevention and treatment of respiratory disease.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"236"},"PeriodicalIF":2.8,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11970004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of optimized lentivirus-like particles for gene editing tool delivery with Gag-Only strategy.","authors":"Jinlin Jia, Yanzhe Hao, Lu Zhang, Xiaofang Cao, Lisha An, Hu Wang, Qi Ma, Xiaohua Jin, Xu Ma","doi":"10.1186/s40001-025-02499-2","DOIUrl":"10.1186/s40001-025-02499-2","url":null,"abstract":"<p><strong>Background: </strong>The development of gene editing tools such as CRISPR-Cas9 and base editors (BE) is critical for genetic diseases and cancer. Lentivirus-like particles (LVLPs) grows into an auspicious platform for delivering mRNA or ribonucleic proteins (RNPs) due to it integrates the advantage of viral and non-viral vectors. Current LVLP systems predominantly utilize HIV-Gag and Pol proteins. However, the reverse transcriptase and integrase of Pol, pose risks of genomic integration and potential tumorigenesis. Enhancing the safety of VLP system is essential. This study focuses on improving the LVLP to minimize these risks.</p><p><strong>Methods: </strong>We implemented a Gag-Only strategy, constructing LVLPs with HIV-Gag protein, thereby eliminating the integration risks linked to Pol. By leveraging the interactions between MS2-MCP (MS2 coat protein), PP7 and PP7 BP (PP7 binding protein), and the psi (HIV packaging signal) with HIV-Gag, we encapsulated PAMless andesine base editor (CE-8e-SpRY) mRNA and sgRNA targeting the PD1 start codon (ATG) into the LVLP. Using recombinant lentiviral vector technology, we developed a stable PD1-expressing 293T cell line (PD1-293T) to assess the editing efficiency of LVLP.</p><p><strong>Results: </strong>The psi-LVLP demonstrated effective packaging capabilities, achieving 15% base editing efficiency in 293T cells. By optimizing plasmid ratios, we observed increased CE-8e-SpRY mRNA copy numbers, with 30% base editing efficiency. Additionally, the integration of HDVrz (hepatitis delta virus ribozyme) and psi into sgRNA (HDVrz-psi-LVLP) substantially enhanced sgRNA copy numbers, resulting in approximately 50% base editing efficiency in 293T cells and 20% base editing efficiency in Jurkat cells. Mendelian randomization analyses revealed significant genetic correlations between PD1, B2M, CIITA, and TIGIT genes with various cancer risks. Furthermore, HDVrz-psi-LVLPs targeting the start codons of B2M, CIITA, and TIGIT exhibited high base editing activity in both Jurkat and 293T cells.</p><p><strong>Conclusion: </strong>In conclusion, this optimized platform effectively encapsulates CE-8e-SpRY mRNA and sgRNA, achieving high editing efficiencies across multiple genes and cell types. We introduce a safer and more efficient gene editing tool delivery system by constructing LVLPs based on the Gag-Only strategy. Our study presents a promising implication for cancer immunotherapy.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"242"},"PeriodicalIF":2.8,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unilateral biportal endoscopic lumbar interbody fusion assisted by a Tianji robot for lumbar degenerative disease in elderly patients: a retrospective study.","authors":"Guofang Fang, Jin Zhang, Hailun Zhu, Xunwei Lai, Jianchang Wu, Xiuwang Li, Zhouxu Hou, Fangxin Chen, Hongxun Sang","doi":"10.1186/s40001-025-02433-6","DOIUrl":"10.1186/s40001-025-02433-6","url":null,"abstract":"<p><strong>Background: </strong>Unilateral biportal endoscopic lumbar interbody fusion (UBE-LIF) has been successfully used to treat degenerative lumbar spinal diseases. Nevertheless, the duration of the UBE-LIF procedure notably exceeds that of minimally invasive transforaminal lumbar interbody fusion (Mis-TLIF), increasing the potential for perioperative complications, particularly in elderly patients.</p><p><strong>Objective: </strong>This retrospective study aimed to compare the results of robot assistance (RA) and non-assistance (NA) groups and to explore the benefits of UBE-LIF assisted by a Tianji robot in aged patients.</p><p><strong>Methods: </strong>60 patients were divided into two groups: 30 patients in the RA group and 30 in the NA group from January 2022 to June 2023. The surgical duration, estimated intraoperative blood loss, postoperative drainage, length of hospital stays, and radiation exposure were examined and documented. Clinical assessments, including the Oswestry Disability Index (ODI), visual analog scale (VAS), modified MacNab criteria, postoperative complications, and interbody fusion rate, were also evaluated.</p><p><strong>Results: </strong>No significant differences were observed between the two groups in terms of postoperative drainage, length of postoperative hospital stay, or fusion rate. However, the RA group exhibited lower perioperative complications, estimated intraoperative blood loss, and duration of radiation exposure than the NA group. The average total operation time in the RA group was 105.3 ± 25.8 min, which was significantly shorter than the NA group's average of 130.5 ± 22.5 min (P < 0.001). Furthermore, both groups demonstrated improvements in all clinical outcomes at various postoperative time points, with no significant differences between them (P > 0.05).</p><p><strong>Conclusions: </strong>Compared with the NA approach, robot-assisted UBE-LIF technology provides accurate intraoperative guidance and helps spinal surgeons achieve accurate decompression. Furthermore, it can reduce radiation exposure, operation time, blood loss, and surgical complications, thereby improving the surgical efficiency and safety.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"231"},"PeriodicalIF":2.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inducing mononuclear cells of patients with CADASIL to construct a CSVD disease model.","authors":"Zhiqiang Wang, Jianjian Yin, Wa Chao, Xiaoning Zhang","doi":"10.1186/s40001-025-02491-w","DOIUrl":"10.1186/s40001-025-02491-w","url":null,"abstract":"<p><strong>Objective: </strong>To produce pluripotent stem cells from peripheral blood mononuclear cells (PBMCs) of a patient with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) and culture and differentiate them into vascular organoids, producing a disease model for cerebral small vessel disease (CSVD).</p><p><strong>Methods: </strong>(1) PMBCs from patients clinically diagnosed with CADASIL (NOTCH3 p.R141C) were induced to differentiate into pluripotent stem cells (iPSCs); the quality and differentiation ability of the iPSCs were determined. (2) CADASIL-derived iPSCs and control iPSCs were cultured and differentiated into vascular organoids. The differences in the morphological structure of the two differentiated groups of vascular organoids were observed, and both were identified.</p><p><strong>Results: </strong>(1) No mycoplasma infections were detected in the iPSCs prepared from the PBMCs of patients with CADASIL. The short tandem repeat (STR) identification verified that the iPSCs originated from the patient, and the karyotype was normal. Flow cytometry and immunofluorescence detection revealed that the iPSCs expressed SSEA4, OCT4, and NANOG stem proteins. Tri-germ differentiation testing confirmed that the iPSCs expressed the endoderm markers SOX17 and FOXA2, the mesoderm markers Brachyury and α-SMA, and the ectoderm markers Pax6 and β-III Tubulin. (2) CADASIL-derived iPSCs and control iPSCs were induced to differentiate and produce endothelial networks and vascular networks, ultimately forming vascular organoids. Compared with control vascular organoids, CADASIL vascular organoids exhibited lower growth density, earlier blood vessel sprouting, longer and thinner vascular filaments, and smaller final vascular organoids. The vascular organoids from the two sources expressed the endothelial cell marker CD31, the vascular smooth muscle marker α-SMA, and the pericyte marker PDGFR-β.</p><p><strong>Conclusion: </strong>Reprogramming technology can be used to induce PBMCs to become iPSCs, and a CSVD disease model can be successfully constructed by culturing and differentiating the iPSCs into CADASIL vascular organoids. The NOTCH3 p.R141C mutation suppresses the vascular differentiation process in CADASIL.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"227"},"PeriodicalIF":2.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis of altered gray matter volume in acute lymphoblastic leukemia patients' postchemotherapy via the AES-SDM.","authors":"Xuelian Zong, Huiping Liu, Xiaoyun Zhao","doi":"10.1186/s40001-025-02461-2","DOIUrl":"10.1186/s40001-025-02461-2","url":null,"abstract":"<p><strong>Background: </strong>Voxel-based morphometry (VBM) reveals diverse alterations in gray matter volume in acute lymphoblastic leukemia (ALL) patients after chemotherapy. However, the reported results are inconsistent. Therefore, our objective was to conduct a meta-analysis to synthesize findings from existing VBM studies and identify consistent patterns of altered gray matter volume in ALL patients post-chemotherapy.</p><p><strong>Materials and methods: </strong>A systematic search was conducted across PubMed, Web of Knowledge, Embase, Google Scholar, and CNKI for VBM studies that compared ALL patients post-chemotherapy with healthy controls (HCs) up to April 1, 2024. Significant cluster coordinates were extracted for comprehensive analysis.</p><p><strong>Results: </strong>We included 7 studies involving 143 ALL patients post-chemotherapy and 140 HCs. ALL patients who underwent chemotherapy presented decreased gray matter volume in the left caudate nucleus, left calcarine fissure/surrounding cortex, left precentral gyrus and right anterior thalamic projections. Jackknife sensitivity analysis validated the robustness of these findings.</p><p><strong>Conclusions: </strong>This meta-analysis revealed consistent gray matter volume alterations in ALL patients post-chemotherapy, emphasizing the need to explore their underlying mechanisms and long-term effects on cognitive and neurological health.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"230"},"PeriodicalIF":2.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPV11E6/E7 induces nasal epithelial hyperplasia through JAK2/STAT3 signaling pathway.","authors":"Yi Zhang, Kaisai Tian, Liying Zheng, Gaohan Zhu, Runyu Zhao, Enhui Zhou, Xiaocheng Xue, Shuixian Huang, Xiaoping Chen, Baoji Hu, Wenhao Yao","doi":"10.1186/s40001-025-02496-5","DOIUrl":"10.1186/s40001-025-02496-5","url":null,"abstract":"<p><strong>Objectives: </strong>Nasal mucosal epithelial hyperplasia can cause nasal hyperplastic diseases, more studies have confirmed that different subtypes of HPV infection play a significant role in nasal proliferative diseases, especially nasal inverted papilloma (NIP). This study aims to elucidate the role and mechanism of the HPV11 subtype in regulating nasal epithelial hyperplasia.</p><p><strong>Methods: </strong>In our previous study, the expression of HPV infection in NIP was analyzed by Flow-through hybridization and gene chip (HybridMax), with the highest expression rate observed for the HPV11 subtype. Therefore, we aimed to overexpress HPV11E6/E7 in nasal mucosal epithelial cells (HNEpC) to verify the regulatory role and mechanism of HPV11 in nasal epithelial hyperplasia at the cellular level. In this manuscript, we constructed a lentiviral vector overexpressing HPV11E6/E7 and transfected it into HNEpC. We used HNEpC as the control group and HPV11E6/E7-overexpressing cells as the experimental group. Cell proliferation was assessed using CCK-8, EdU, and colony formation assays. Cell migration ability was evaluated by wound healing and Transwell assays. Protein expression levels related to apoptosis, epithelial-mesenchymal transition (EMT), and the JAK2/STAT3 pathway were analyzed by western blot.</p><p><strong>Results: </strong>The results showed that overexpression of HPV11E6/E7 significantly increased the proliferation and migration of nasal epithelial cells, promoted the progression of EMT, and inhibited cell apoptosis. Further verification showed that the overexpression of HPV11E6/E7 significantly promoted the activation of the JAK2/STAT3 signaling pathway.</p><p><strong>Conclusions: </strong>In summary, we found that low-risk subtype HPV11 promotes nasal mucosal epithelial hyperplasia and malignant progression by increasing activation of the JAK2/STAT3 pathway. The JAK2/STAT3 pathway has been prioritized due to its established role in promoting cell proliferation and EMT in HPV-related diseases.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"224"},"PeriodicalIF":2.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between periodontitis and gastrointestinal cancer risk and prognosis: evidence from a nested case-control study in Southwest China.","authors":"Ting Luo, Juan Li, Ke Pu, Guodong Yang","doi":"10.1186/s40001-025-02508-4","DOIUrl":"10.1186/s40001-025-02508-4","url":null,"abstract":"<p><strong>Background: </strong>With low early detection rates and high incidence and mortality, Gastrointestinal cancer (GIC) imposes a significant global health burden. Emerging evidence indicates that periodontitis may be a potential risk factor for GIC development; however, epidemiological data remains inconclusive.</p><p><strong>Objective: </strong>This study aimed to examine the impact of periodontitis on the incidence, recurrence, and metastasis of GIC in Southwest China, thereby offering epidemiological evidence to support GIC prevention and management.</p><p><strong>Methods: </strong>Between September 2022 and August 2024, a case-control study was conducted at the Affiliated Hospital of North Sichuan Medical College. Five hundred GIC patients were included as the case group based on the predefined inclusion and exclusion criteria, while 1005 healthy individuals were recruited for the control group. Multivariate analyses were performed to examine the associations between periodontitis and GIC incidence, recurrence, and metastasis while controlling for potential confounding factors.</p><p><strong>Results: </strong>The results of this study demonstrated that periodontitis was significantly associated with the incidence of esophageal, gastric, and colorectal cancer. Even after adjusting for potential confounders, it remained a significant risk factor for esophageal cancer (OR = 2.810, 95% CI 1.032-7.649, P = 0.043), colon cancer (OR = 2.330, 95% CI 1.072-5.067, P = 0.033), and rectal cancer (OR = 2.730, 95% CI 1.247-5.379, P = 0.012). Compared to non-periodontitis subjects, periodontitis showed a significant association with distant metastasis of rectal cancer (aHR = 5.332, 95% CI 1.406-20.220, P = 0.014). Moreover, severe periodontitis was identified as an risk factor for distant metastasis in rectal cancer (aHR = 10.138, 95% CI 1.824-56.354, P = 0.008).</p><p><strong>Conclusion: </strong>This study highlights significant associations between periodontitis and an increased risk of esophageal and colorectal cancers. Additionally, patients with rectal cancer and periodontitis exhibited an increased risk of distant metastasis compared to those without periodontitis.</p>","PeriodicalId":11949,"journal":{"name":"European Journal of Medical Research","volume":"30 1","pages":"225"},"PeriodicalIF":2.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}