Advanced Science最新文献_第9页

A Modular and Customizable CRISPR/Cas Toolkit for Epigenome Editing of Cis-regulatory Modules. 用于顺式调控模块表观基因组编辑的模块化和可定制CRISPR/Cas工具包。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-09-29 DOI: 10.1002/advs.202503917

Lingrui Zhang, Jianxin Fu, Tiandan Long, Chao Zhang, Fuhua Fan, Zhaobo Lang, Jian-Kang Zhu

{"title":"A Modular and Customizable CRISPR/Cas Toolkit for Epigenome Editing of Cis-regulatory Modules.","authors":"Lingrui Zhang, Jianxin Fu, Tiandan Long, Chao Zhang, Fuhua Fan, Zhaobo Lang, Jian-Kang Zhu","doi":"10.1002/advs.202503917","DOIUrl":"https://doi.org/10.1002/advs.202503917","url":null,"abstract":"Epigenome and cis-regulome, comprising cis-regulatory elements (CREs) and modules (CRMs), jointly define the architecture of gene regulation. However, the causal mechanisms by which epigenetic marks influence CRM function remain elusive. To address this, modular epigenome editing frameworks, exemplified by dead Cas9-coupled DNA demethylation (dCd) and DNA methylation (dCm) platforms, are developed for programmable dissection and engineering of CRM activity. The dCd system modulates methylation levels and transcriptional output at CRMs in situ or ex situ, in accordance with CRM-specific methylation responsiveness, and alters co-transcriptional RNA processing to yield predictable phenotypic outcomes in plants. These findings underscore the reliability of targeted DNA demethylation. In parallel, the dCm system reconstitutes methylation-dependent and -sensitive CRMs of diverse origins in Saccharomyces cerevisiae, a species devoid of native DNA methylation, enabling causal dissection of epigenetic regulation and revealing cross-species portability. This system further uncovers crosstalk between DNA methylation and chromatin modifications, and enables logic-gated control of endogenous genes through CRM engineering. Incorporation of optogenetic and temperature-sensitive anti-CRISPR inhibitors confers tunable, reversible regulation, proposing dCm as a foundation for input-responsive synthetic epigenome editors. Together, these frameworks provide a versatile platform to decode and reprogram cis-regulatory epigenetic logic, with broad applications in trait design and synthetic biology.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e03917"},"PeriodicalIF":14.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145190468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BROAD-NESS Uncovers Dual-Stream Mechanisms Underlying Predictive Coding in Auditory Memory Networks. 广谱揭示听觉记忆网络预测编码的双流机制。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-09-29 DOI: 10.1002/advs.202507878

Leonardo Bonetti, Gemma Fernández-Rubio, Mathias H Andersen, Chiara Malvaso, Francesco Carlomagno, Claudia Testa, Peter Vuust, Morten L Kringelbach, Mattia Rosso

{"title":"BROAD-NESS Uncovers Dual-Stream Mechanisms Underlying Predictive Coding in Auditory Memory Networks.","authors":"Leonardo Bonetti, Gemma Fernández-Rubio, Mathias H Andersen, Chiara Malvaso, Francesco Carlomagno, Claudia Testa, Peter Vuust, Morten L Kringelbach, Mattia Rosso","doi":"10.1002/advs.202507878","DOIUrl":"https://doi.org/10.1002/advs.202507878","url":null,"abstract":"Whole-brain network dynamics are yet to be fully understood, particularly in the context of auditory memory and predictive coding. BROAD-NESS (BROADband brain Network Estimation via Source Separation), a novel pipeline for extracting broadband whole-brain networks from source-reconstructed MEG data is introduced. During auditory sequence recognition, BROAD-NESS identified two orthogonal networks centered on auditory cortices. The first, also encompassing medial cingulate, is primarily involved in processing sounds and shows consistent but less marked differences between experimental conditions. The second, involving hippocampus, anterior cingulate, insula, and inferior temporal regions, exhibits strong condition-dependent dynamics, reflecting engagement in confirmed predictions and prediction errors. The networks differ in temporal dynamics, spatial gradients, and behavioral relevance. Phase space and recurrence quantification analysis (RQA) reveal that more recurrent and stable dynamics are linked to higher accuracy and faster responses across sequence types. BROAD-NESS also enables direct PCA versus ICA comparison, showing PCA-based networks to be more robust and interpretable. Conceptually, this work reveals a dual-stream embedded organization of auditory memory networks that mirrors, yet functionally diverges from, visual pathways. Methodologically, it introduces BROAD-NESS, a powerful and interpretable pipeline for characterizing the spatiotemporal architecture of brain networks in neurophysiology.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e07878"},"PeriodicalIF":14.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Iron Nanoparticles on Porous Carbon Discs: Electrocatalysts with Over 30% Energy Efficiencies for the Production of Ammonia from Nitrate. 多孔碳片上的铁纳米颗粒：从硝酸盐中生产氨的电催化剂的能源效率超过30%。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-09-29 DOI: 10.1002/advs.202514504

Daon Park, Jinuk Choi, Ji Yong Shim, Yoon Kee Kim, Jina Park, Chang Wan Kang, So Young Park, Tae-Yong An, Hyojung Lim, Subramani Surendran, Kyoung Chul Ko, Uk Sim, Seung Uk Son

引用次数: 0

Microneedle-Based Biofuel Cell with MXene/CNT Hybrid Bioanode: Fundamental and Biomedical Application. 基于微针的MXene/CNT混合生物阳极生物燃料电池：基础和生物医学应用。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-09-29 DOI: 10.1002/advs.202516229

Shoujie Guan, Jingxi Wang, Yang Yang, Xun Zhu, Jianping Zhou, Dingding Ye, Rong Chen, Qinlin Fan, Qiang Liao

{"title":"Microneedle-Based Biofuel Cell with MXene/CNT Hybrid Bioanode: Fundamental and Biomedical Application.","authors":"Shoujie Guan, Jingxi Wang, Yang Yang, Xun Zhu, Jianping Zhou, Dingding Ye, Rong Chen, Qinlin Fan, Qiang Liao","doi":"10.1002/advs.202516229","DOIUrl":"https://doi.org/10.1002/advs.202516229","url":null,"abstract":"Biofuel cells show emerging potential as energy-supply platform by harvesting bioenergy from human biofluids for next-generation self-powered transdermal drug delivery systems. Herein, the study proposes the hydrogel-based microneedle patch as the drug penetration unit within the corneum to drive the large drug molecules' delivery. Benefiting from the high porosity and swelling capability, the interstitial fluid can be extracted efficiently to the body surface. One biofuel cell is integrated into the microneedle patch with MXene/carbon nanotube functionalized glucose oxidase bioanode. The transdermal currents of 4-5 µA and open-circuit potential of 0.28 V are collected in in vivo. The transdermal delivery efficiency and releasing rate of ciprofloxacin and insulin are significantly enhanced in vitro and in vivo models, respectively. The theoretical simulations provide detailed insights into the good electron transport and substrate adsorption, glucose replenishment, and drug delivery under electric field, benefiting the development of transdermal drug delivery systems.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e16229"},"PeriodicalIF":14.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145190426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laeverin is Cell-Surface Target for Liquid-Phase Metastasizing Cancer Cells. Laeverin是癌细胞液相转移的细胞表面靶点。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-09-29 DOI: 10.1002/advs.202511349

Haruki Kasama, Yuya Sakai, Kyosuke Kagami, Takashi Iizuka, Tatsuhito Kanda, Takuma Suzuki, Kayo Kayahashi, Masanori Ono, Tomoko Fujiwara, Shintaro Yagi, Noriyuki Inaki, Isao Matsumoto, Rena Yamazaki, Kaoru Abiko, Noriomi Matsumura, Akira Hattori, Takiko Daikoku, Hiroshi Fujiwara

{"title":"Laeverin is Cell-Surface Target for Liquid-Phase Metastasizing Cancer Cells.","authors":"Haruki Kasama, Yuya Sakai, Kyosuke Kagami, Takashi Iizuka, Tatsuhito Kanda, Takuma Suzuki, Kayo Kayahashi, Masanori Ono, Tomoko Fujiwara, Shintaro Yagi, Noriyuki Inaki, Isao Matsumoto, Rena Yamazaki, Kaoru Abiko, Noriomi Matsumura, Akira Hattori, Takiko Daikoku, Hiroshi Fujiwara","doi":"10.1002/advs.202511349","DOIUrl":"https://doi.org/10.1002/advs.202511349","url":null,"abstract":"Laeverin (LVRN) is a cell-surface immunoregulatory factor that is specifically expressed in embryo-derived extravillous trophoblast, which invades maternal spiral arteries without immune rejection during human placentation. Here, it is found that various epithelial cancer cell lines upregulated LVRN expression in association with the expression of POU5F1 after spheroid formation under floating conditions. Immunohistochemically, LVRN expression is detected in the lesions of vascular, lymphatic, and peritoneal invasion of ovarian, cervical, endometrial, breast, and lung cancers. LVRN-positive circulating tumor cells are also identified in the blood of uterine cervical and endometrial cancers, showing that LVRN expression is induced in cancer cells in the distant metastatic phase. Monomethylauristatin E-conjugated anti-LVRN antibody induced cell death in ovarian cancer-derived cell lines in the liquid phase in vitro and inhibited their peritoneal dissemination in nude mice in vivo. These findings indicate that LVRN is a unique and promising cell-surface target molecule for liquid-phase metastasizing cancer cells.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e11349"},"PeriodicalIF":14.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145190479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly Efficient Air Sterilization via Low-Temperature Interfacial Evaporation in Inductively Heated Superhydrophilic Ferromagnetic Filters. 感应加热超亲水性铁磁过滤器低温界面蒸发高效空气灭菌。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-09-28 DOI: 10.1002/advs.202509118

Arnau Fons, Cristina Vaca, Eloi Franco-Trepat, Aritz Lafuente, José Luis Tajada, Albert Serrà, Jorge Diaz Pedroza, Sandra Franco, Rytis Boreika, Itziar Erkizia, Nuria Izquierdo-Useros, Alberto López-Ortega, Eneko Garaio, Maria Jose Esplandiu, Josep Nogues, Borja Sepúlveda

{"title":"Highly Efficient Air Sterilization via Low-Temperature Interfacial Evaporation in Inductively Heated Superhydrophilic Ferromagnetic Filters.","authors":"Arnau Fons, Cristina Vaca, Eloi Franco-Trepat, Aritz Lafuente, José Luis Tajada, Albert Serrà, Jorge Diaz Pedroza, Sandra Franco, Rytis Boreika, Itziar Erkizia, Nuria Izquierdo-Useros, Alberto López-Ortega, Eneko Garaio, Maria Jose Esplandiu, Josep Nogues, Borja Sepúlveda","doi":"10.1002/advs.202509118","DOIUrl":"https://doi.org/10.1002/advs.202509118","url":null,"abstract":"The scientific evidence supporting airborne transmission of pathogens in closed spaces highlights the inefficiency of current air circulation and filtration technologies (e.g., HEPA filters, UV, ozone, ionization) in preventing the spread of airborne pathogens. This underscores the urgent need for new air disinfection devices. Here, the first air sterilization technology is presented based on low-temperature interfacial evaporation. This novel approach integrates superhydrophilic micro/nano-structured stainless-steel filters and ultra-efficient magnetic inductive heating to enable complete evaporation of water from the contaminated aerosols and the precipitation of all the organic and inorganic residues within the filter at temperatures in the range of 60-80 °C. The technology is validated through experiments with contaminated aerosols with different active viruses, including SARS-CoV-2 and respiratory syncytial virus (RSV), demonstrating the elimination of 99.6% or more of the nebulized viruses, at filter temperatures of 60-80 °C and airflow rates of 15 L min-1. The filters also support pyrolytic self-cleaning and reuse, ensuring extended service time and minimal maintenance. This air sterilization technology represents a significant advancement over existing state-of-the-art filtering technology, offering unmatched versatility, low energy consumption, and cost-effective sterilization, without generating harmful radicals, dangerous high voltages, or high temperatures.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e09118"},"PeriodicalIF":14.1,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Multifrequency Modulated Wireless Information and Power Transfer for a Triboelectronic Monitoring System. 摩擦电子监测系统的同步多频调制无线信息和功率传输。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-09-28 DOI: 10.1002/advs.202510427

Hongwei Yuan, Youngwook Chung, Ze Wang, Haojie Li, He Zhang, Sang-Woo Kim, Keren Dai

{"title":"Simultaneous Multifrequency Modulated Wireless Information and Power Transfer for a Triboelectronic Monitoring System.","authors":"Hongwei Yuan, Youngwook Chung, Ze Wang, Haojie Li, He Zhang, Sang-Woo Kim, Keren Dai","doi":"10.1002/advs.202510427","DOIUrl":"https://doi.org/10.1002/advs.202510427","url":null,"abstract":"Ultrasound-driven triboelectric nanogenerators (US-TENGs) address energy supply challenges for implantable medical microsystems, underwater sensing and monitoring microsystems, etc., and are achieving increasingly important applications. In this work, multifrequency modulated simultaneous wireless power and information transmission (MF-SWIPT) technology is proposed for a US-TENG that allows both the power and information flows to be transmitted continuously without interruption. The deliberately optimized design traditionally allows complex multifrequency information demodulation circuits to consume only a few microwatts of power, making it possible to operate in the US-TENG system. Benefiting from this new architecture, the efficiency of information transmission is increased, with almost no reduction in the power transmission efficiency. The US-TENG-MF-SWIPT is demonstrated for underwater temperature monitoring microsystems and other applications; in addition to powering the microsystem for continuous operation, it is capable of transmitting information and text and further enables the modulation of microsystem operating parameters, such as alarm temperature thresholds. Therefore, US-TENG-MF-SWIPT technology will greatly enhance the energy sustainability and information interaction of these microsystems.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e10427"},"PeriodicalIF":14.1,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Metabolic and Inflammatory Clustering Reveals Distinct Risk Profiles for Digestive Diseases. 综合代谢和炎症聚类揭示了消化系统疾病的独特风险概况。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-09-28 DOI: 10.1002/advs.202511000

Zhenhe Jin, Qichen Chen, Liangfeng Zhou, Kexin Ye, Zhaoxue Liu, Wenxi Jiang, Linwen Luo, Yize Wang, Xiaohua Ye, Chaohui Yu, Zhe Shen

{"title":"Integrated Metabolic and Inflammatory Clustering Reveals Distinct Risk Profiles for Digestive Diseases.","authors":"Zhenhe Jin, Qichen Chen, Liangfeng Zhou, Kexin Ye, Zhaoxue Liu, Wenxi Jiang, Linwen Luo, Yize Wang, Xiaohua Ye, Chaohui Yu, Zhe Shen","doi":"10.1002/advs.202511000","DOIUrl":"https://doi.org/10.1002/advs.202511000","url":null,"abstract":"Emerging research highlights the complex relationship between metabolic dysfunction and chronic low-grade inflammation, which disrupts gut homeostasis and drives disease progression. However, most current studies evaluate metabolic and inflammatory markers separately, relying on basic indicators such as body mass index (BMI) or individual biomarkers. In this study, a scalable clustering framework is developed to integrate six clinical parameters in 398 432 participants from the UK Biobank, identifying four distinct metabolic-inflammatory subtypes. Cox proportional hazards models demonstrate significant associations between these subtypes and digestive disease risk. Using 251 plasma metabolites and elastic net regression, cluster-associated metabolite signatures are identified. Mediation analyses indicate that metabolic signatures mediate the association between clusters and digestive disease risk. Machine learning algorithms are applied to construct disease-specific metabolic risk scores, achieving C-indices above 0.70 for ten digestive disease endpoints. Explainable machine learning approaches further identify both shared and disease-specific predictors, with glycoprotein acetyls, valine, tyrosine, and fatty acids emerging as key risk indicators. This integrative approach provides a comprehensive framework for digestive disease risk assessment and offers novel insights into the metabolic mechanisms underlying disease susceptibility.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e11000"},"PeriodicalIF":14.1,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and Functional Versatility of the Amyloidogenic Non-Amidated Variant of the Antimicrobial Peptide Citropin 1.3. 抗菌肽Citropin 1.3淀粉样变性非修饰变体的结构和功能通用性。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-09-28 DOI: 10.1002/advs.202503997

Fabio Strati, Mariana Pigozzi Cali, Yehudi Bloch, Siavash Mostafavi, Jim Monistrol, Aleksandr Golubev, Bader Rayan, Emil Gustavsson, Meytal Landau

引用次数: 0

Multispectral Chiral Quasi-Bound States in the Continuum Enabled Microfluidics for High-Throughput Molecular Screening and Quantification. 连续介质中多光谱手性准束缚态微流体的高通量分子筛选和定量。

IF 14.1 1区材料科学

Advanced Science Pub Date : 2025-09-28 DOI: 10.1002/advs.202515443

Xinyue Liang, Zihan Zhao, Xiaocong Tang, Haiyue Yang, Lanju Liang, Meng Zhao, Cong Wang, Lei Wang, Xumin Ding

{"title":"Multispectral Chiral Quasi-Bound States in the Continuum Enabled Microfluidics for High-Throughput Molecular Screening and Quantification.","authors":"Xinyue Liang, Zihan Zhao, Xiaocong Tang, Haiyue Yang, Lanju Liang, Meng Zhao, Cong Wang, Lei Wang, Xumin Ding","doi":"10.1002/advs.202515443","DOIUrl":"https://doi.org/10.1002/advs.202515443","url":null,"abstract":"Traditional chiral detection methods often suffer from label dependence and limited throughput, hindering accurate profiling of trace enantiomers. Herein, a terahertz (THz) multispectral metachip-enabled microfluidic platform is presented for label-free, high-throughput screening and quantitation of chiral molecules. The metachip integrates arrayed pixels engineered with chiral quasi-bound states in the continuum (q-BIC), generating high circular dichroism (CD) across 0.5-2.0 THz. On-chip microfluidic integration enables multi-dimensional CD feature extraction in aqueous environments, while the Uniform Manifold Approximation and Projection (UMAP) algorithm maps multidimensional CD features into a Two-dimensional (2D) spectrum, simultaneously realizing conformation identification and concentration quantification (0.05-0.3 mg dL-1). Experimentally, 87.5% discrimination accuracy is achieved for 8 chiral biomolecules in aqueous solutions (including amino acids and dipeptides) with state-of-the-art sensitivity (2.1024 THz· mg1 ·dL). This platform bridges the gap between high-sensitivity chiral sensing and real-time fluidic analysis, offering a transformative tool for trace enantiomer characterization in clinical diagnostics and drug development.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e15443"},"PeriodicalIF":14.1,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0