Advanced Science最新文献_第9页

In Situ Control of Reactive Mesogens Alignment During 3D Printing by Two-Photon Lithography.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202415159

Tiziana Ritacco, Alfredo Mazzulla, Michele Giocondo, Gabriella Cipparrone, Pasquale Pagliusi

{"title":"In Situ Control of Reactive Mesogens Alignment During 3D Printing by Two-Photon Lithography.","authors":"Tiziana Ritacco, Alfredo Mazzulla, Michele Giocondo, Gabriella Cipparrone, Pasquale Pagliusi","doi":"10.1002/advs.202415159","DOIUrl":"https://doi.org/10.1002/advs.202415159","url":null,"abstract":"Photopolymerizable liquid crystals, also known as reactive mesogens, are leading candidates for additive manufacturing of smart microdevices via two-photon lithography (TPL). While substantial advancements are made toward innovative applications, precise control of molecular alignment during fabrication, essential for tailoring complex optical and mechanical responses, remains a significant challenge. Current solutions require elaborate multi-step procedures or customized setups to achieve 2D or 3D alignment patterns. Herein, the deterministic effect of TPL on the orientation of mesogenic moieties is reported, under optimized printing conditions. Specifically, a single-step simple method is developed for aligning the nematic director in situ, with sub-diffraction-limited resolution, during 3D printing. Based on the conventional TPL workflow, the \"director-tuning mode\" (DiTuM) relies on the anisotropic photopolymerization reaction occurring along the print path at low laser scan speeds (≈0.1mm s-1). A TPL-induced \"easy axis\" arises for the mesogenic moieties, programmable in direction and strength, and competes with the initial alignment to create potentially convolute 3D director fields. The method holds considerable promise for 3D/4D printing, enabling advanced functionalities, and offers a robust platform for anti-counterfeiting applications, leveraging the unique optical signatures generated by complex microstructures.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2415159"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vivo Reprogramming of Tissue-Derived Extracellular Vesicles for Treating Chronic Tissue Injury Through Metabolic Engineering.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202415556

Meng Zhao, Shuyun Liu, Yizhuo Wang, Peng Lou, Ke Lv, Tian Wu, Lan Li, Qianyi Wu, Jiaying Zhu, Yanrong Lu, Meihua Wan, Jingping Liu

{"title":"In Vivo Reprogramming of Tissue-Derived Extracellular Vesicles for Treating Chronic Tissue Injury Through Metabolic Engineering.","authors":"Meng Zhao, Shuyun Liu, Yizhuo Wang, Peng Lou, Ke Lv, Tian Wu, Lan Li, Qianyi Wu, Jiaying Zhu, Yanrong Lu, Meihua Wan, Jingping Liu","doi":"10.1002/advs.202415556","DOIUrl":"https://doi.org/10.1002/advs.202415556","url":null,"abstract":"Extracellular vesicles (EVs) have emerged as promising therapeutics for regenerative medicine, but the efficacy of current exogenous EV-based therapies for treating chronic tissue injury is still unsatisfactory. Exercise can affect skeletal muscle EV secretion and that this process regulates the systemic health-promoting role of exercise, suggesting that fine-tuning of endogenous tissue EV secretion may provide a new therapeutic avenue. Here, this work reports that in vivo reprogramming of EV secretion via metabolic engineering is a promising strategy for treating chronic diseases. Briefly, exercise enhanced mitochondrial metabolism and EV production in healthy skeletal muscles, and EVs from healthy skeletal muscles subjected to exercise or metabolic engineering (boosting mitochondrial biogenesis via AAV-mediated muscle-specific TFAM overexpression) exerted cellular protective effects in vitro. In injured skeletal muscles, in vivo metabolic engineering therapy could reprogram EV secretion patterns (reducing pathological EV compositions while increasing beneficial EV compositions) by regulating multiple EV biogenesis and cargo sorting pathways. Reprogrammed muscle-derived EVs could reach major organs and tissues via the circulation and then simultaneously attenuated multiple-tissue (e.g., muscle and kidney) injury in chronic kidney disease. This study highlights that in vivo reprogramming of tissue-derived EVs via a metabolic engineering approach is a potential strategy for treating diverse chronic diseases.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2415556"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HDAC6 and USP9X Control Glutamine Metabolism by Stabilizing GS to Promote Glioblastoma Tumorigenesis.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202501553

Go Woon Kim, Minhae Cha, Hien Thi My Ong, Jung Yoo, Yu Hyun Jeon, Sang Wu Lee, Soo Yeon Oh, Min-Jung Kang, Youngsoo Kim, So Hee Kwon

{"title":"HDAC6 and USP9X Control Glutamine Metabolism by Stabilizing GS to Promote Glioblastoma Tumorigenesis.","authors":"Go Woon Kim, Minhae Cha, Hien Thi My Ong, Jung Yoo, Yu Hyun Jeon, Sang Wu Lee, Soo Yeon Oh, Min-Jung Kang, Youngsoo Kim, So Hee Kwon","doi":"10.1002/advs.202501553","DOIUrl":"https://doi.org/10.1002/advs.202501553","url":null,"abstract":"Glioblastoma (GBM) is the most common and the deadliest brain cancer. Glutamine anabolism mediated by glutamine synthetase (GS) is beneficial for GBM cell growth, especially under glutamine deprivation. However, the molecular mechanism underlying GS homeostasis in GBM remains undisclosed. Here, it is reported that histone deacetylase 6 (HDAC6) promotes GS deacetylation, stabilizing it via ubiquitin-mediated pathway. It is found that deubiquitination of GS is modulated by ubiquitin-specific peptidase 9, X-linked (USP9X). USP9X stabilizes GS by removing its K48-linked polyubiquitination on lysine 91 and 103. Accordingly, targeting HDAC6 and USP9X in vitro and in vivo represses GBM tumorigenesis by decreasing GS stability. Metabolic analysis shows that silencing HDAC6 and USP9X disrupts de novo nucleotide synthesis, thereby attenuating GBM cell growth. Furthermore, GS modulation by targeting HDAC6 and USP9X restrains the self-renewal capacity. These results suggest that HDAC6 and USP9X are crucial epigenetic enzymes that promote GBM tumorigenesis by modulating glutamine metabolism.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2501553"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zinc-Doping-Induced Electronic States Modulation of Molybdenum Carbide: Expediting Rate-Determining Steps of Sulfur Conversion in Lithium-Sulfur Batteries.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202417126

Bin Qin, Yanmei Li, Qun Wang, Si Zhang, Jinglin Zhang, Bin Wang, Peijia Wang, Yuhan Chen, Weiqi Yao, Fang Wang

{"title":"Zinc-Doping-Induced Electronic States Modulation of Molybdenum Carbide: Expediting Rate-Determining Steps of Sulfur Conversion in Lithium-Sulfur Batteries.","authors":"Bin Qin, Yanmei Li, Qun Wang, Si Zhang, Jinglin Zhang, Bin Wang, Peijia Wang, Yuhan Chen, Weiqi Yao, Fang Wang","doi":"10.1002/advs.202417126","DOIUrl":"https://doi.org/10.1002/advs.202417126","url":null,"abstract":"Enhancing Li2S deposition and oxidation kinetics in lithium-sulfur batteries, especially the potential-limiting step under lean electrolyte, can be effectively achieved by developing conductive catalysts. In this study, by using ZnMoO4 as precursors, Zn-doped molybdenum carbide microflowers (Zn-Mo2C) composed of speared porous sheets are fabricated with a hierarchically ordered structure. Density functional theory calculations indicate that Zn doping shifts the d-band center on Mo atoms in Mo2C upward, promotes the elevation of certain antibonding orbitals in Mo─S bonds above the Fermi level, enhances d-p interaction between lithium polysulfides (LiPSs) and catalysts, weakens both S─S and Li─S bonds of LiPSs. Incorporating Zn significantly reduces the Gibbs free energy barrier for the rate-limiting step of the Li2S2 → Li2S conversion, from 0.52 eV for Mo2C to just 0.05 eV for Zn-doped Mo2C. Thus, the synthesized Zn-Mo2C demonstrates impressive bifunctional electrocatalytic performance, significantly advancing sulfur reduction and Li2S decomposition. Moreover, this modification enhances charge transfer within the Zn-Mo2C/LiPSs system, synergistically accelerating the kinetics of Li2S4 to Li2S reduction and Li2S oxidation. The Zn-Mo2C/S cathode demonstrates impressive electrochemical performance, achieves remarkable cycling stability with a minimal capacity decay of 0.021% per cycle over 1000 cycles at 5 C, underscoring its potential for high-energy applications.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2417126"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering Interfacial Oxygen-Bridged Binuclear Metal Centers of Heterogenized Molecular Catalyst for Water Electrolysis.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-30 DOI: 10.1002/advs.202417607

Zhou Yu, Jian-Ping Li, Xian-Kun Xu, Zhong-Chen Ding, Xiao-Hui Peng, Yi-Jing Gao, Qiang Wan, Ju-Fang Zheng, Xiao-Shun Zhou, Ya-Hao Wang

{"title":"Uncovering Interfacial Oxygen-Bridged Binuclear Metal Centers of Heterogenized Molecular Catalyst for Water Electrolysis.","authors":"Zhou Yu, Jian-Ping Li, Xian-Kun Xu, Zhong-Chen Ding, Xiao-Hui Peng, Yi-Jing Gao, Qiang Wan, Ju-Fang Zheng, Xiao-Shun Zhou, Ya-Hao Wang","doi":"10.1002/advs.202417607","DOIUrl":"https://doi.org/10.1002/advs.202417607","url":null,"abstract":"The success of different heterogeneous strategies of organometallic catalysts has been demonstrated to achieve high selectivity and activity in photo/electrocatalysis. However, yielding their catalytic mechanisms at complex molecule-electrode and electrochemical interfaces remains a great challenge. Herein, shell-isolated nanoparticle-enhanced Raman spectroscopy is employed to elucidate the dynamic process, interfacial structure, and intermediates of copper hydroxide-2-2' bipyridine on Au electrode ((bpy)Cu(OH)2/Au) during the oxygen evolution reaction (OER). Direct Raman molecular evidences reveal that the interfacial (bpy)Cu(OH)2 oxidizes into Cu(III) and bridges to Au atoms via oxygenated species, forming (bpy)Cu(III)O2-Au with oxygen-bridged binuclear metal centers of Cu(III)-O-Au for the OER. As the potential further increases, Cu(III)-O-Au combines with surface hydroxyl groups (*OH) to form the important intermediate of Cu(III)-OOH-Au, which then turns into Cu(III)-OO-Au to release O2. Furthermore, in situ electrochemical impedance spectroscopy proves that the Cu(III)-O-Au has lower resistance and faster mass transport of hydroxy to enhance OER. Theoretical calculations reveal that the formation of Cu(III)-O-Au significantly modify the elementary reaction steps of the OER, resulting in a lower potential-determining step of ≈0.58 V than that of bare Au. This work provides new insights into the OER mechanism of immobilized-molecule catalysts for the development and application of renewable energy conversion devices.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2417607"},"PeriodicalIF":14.3,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ROS-Activated Nanohydrogel Scaffolds with Multi-Factors Controlled Release for Targeted Dual-Lineage Repair of Osteochondral Defects.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-29 DOI: 10.1002/advs.202412410

Xiuhui Wang, Shunli Wu, Ruiyang Li, Huijian Yang, Yue Sun, Zijie Cao, Xiao Chen, Yan Hu, Hao Zhang, Zhen Geng, Long Bai, Zhongmin Shi, Ke Xu, Hongbo Tan, Jiacan Su

{"title":"ROS-Activated Nanohydrogel Scaffolds with Multi-Factors Controlled Release for Targeted Dual-Lineage Repair of Osteochondral Defects.","authors":"Xiuhui Wang, Shunli Wu, Ruiyang Li, Huijian Yang, Yue Sun, Zijie Cao, Xiao Chen, Yan Hu, Hao Zhang, Zhen Geng, Long Bai, Zhongmin Shi, Ke Xu, Hongbo Tan, Jiacan Su","doi":"10.1002/advs.202412410","DOIUrl":"https://doi.org/10.1002/advs.202412410","url":null,"abstract":"Achieving self-healing for osteochondral defects caused by trauma, aging, or disease remains a significant challenge in clinical practice. It is an effective therapeutic strategy to construct gradient-biomimetic biomaterials that replicate the hierarchical structure and complex microenvironment of osteochondral tissues for dual-lineage regeneration of both cartilage and subchondral bone. Herein, ROS-activated nanohydrogels composite bilayer scaffolds with multi-factors controlled release are rationally designed using the combination of 3D printing and gelatin placeholder methods. The resulting nanohydrogel scaffolds exhibit micro-nano interconnected porous bilayer structure and soft-hard complex mechanical strength for facilitating 3D culture of BMSCs in vitro. More importantly, multi-stage continuous responses of anti-inflammation, chondrogenesis and osteogenesis, are effectively induced via the sequential release of multi-factors, including diclofenac sodium (DS), kartogenin (KGN) and bone morphogenetic protein 2 (BMP-2), from ROS-activated nanohydrogel scaffolds, thereby improved dual-lineage regeneration of cartilage and subchondral bone tissue in the osteochondral defect model of SD rats. These findings suggest that ROS-activated nanohydrogel scaffolds with such specific soft-hard bilayer structure and sequential delivery of functional factors, provides a promising strategy in dual-lineage regeneration of osteochondral defects.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2412410"},"PeriodicalIF":14.3,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyridine-Fused Bis(azacorrole)s: Easily Accessible NIR III Absorbing Cation Radicals and Biradicaloids of Antiaromatic Ground State.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-28 DOI: 10.1002/advs.202416223

Sha Li, Shaowei Zhang, Xiaofang Li, Oskar Smaga, Kinga Szydełko, Miłosz Pawlicki, Piotr J Chmielewski

{"title":"Pyridine-Fused Bis(azacorrole)s: Easily Accessible NIR III Absorbing Cation Radicals and Biradicaloids of Antiaromatic Ground State.","authors":"Sha Li, Shaowei Zhang, Xiaofang Li, Oskar Smaga, Kinga Szydełko, Miłosz Pawlicki, Piotr J Chmielewski","doi":"10.1002/advs.202416223","DOIUrl":"https://doi.org/10.1002/advs.202416223","url":null,"abstract":"A family of pyridine-fused bis(porphyrinoids) is obtained, including constitutionally isomeric bis(azacorrole)s, azacorrole-oxacorrole, as well as azacorrole-norcorrole heterodimers by two distinct synthetic approaches. Spectroscopic characteristics, corroborated by Density Functional Theory (DFT) calculations, indicate aromaticity of the bis(azacorrole) as well as azacorrole-oxacorrole products, while for the azacorrole-norcorrole heterodimers, the presence of both dia- and paratropic currents is detected. Electrochemical analyses indicate facile chemical access to cation radicals and dicationic species that have been characterized by electronic and electron spin resonance spectroscopy as well as by DFT calculations. Monocations give rise to the relatively strong absorption bands in the near infra red (NIR) region between 2400 and 3200 nm, while dications are characterized by a series of absorptions between 1000 and 2200 nm. Electron spin resonance (ESR) experiments indicate the presence of singlet-triplet spin equilibria for the dications. For the dication of bis(azacorrole) of the most planar structure, the singlet ground state is established, and low temperature nuclear magnetic resonance (NMR) as well as gauge-independent atomic orbital NMR calculations indicate its antiaromatic character.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2416223"},"PeriodicalIF":14.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annexin A3 Represses Endothelial Permeability and Inflammation During Sepsis via Actin Cytoskeleton Modulation.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-28 DOI: 10.1002/advs.202416904

Manyu Xing, Shuang Liang, Wei Cao, Qulian Guo, Wangyuan Zou

{"title":"Annexin A3 Represses Endothelial Permeability and Inflammation During Sepsis via Actin Cytoskeleton Modulation.","authors":"Manyu Xing, Shuang Liang, Wei Cao, Qulian Guo, Wangyuan Zou","doi":"10.1002/advs.202416904","DOIUrl":"https://doi.org/10.1002/advs.202416904","url":null,"abstract":"Increased endothelial permeability and a dysregulated inflammatory response play key roles in organ damage in sepsis. The role of annexin A3 (ANXA3) in regulating endothelial permeability and inflammation during sepsis is explored using ANXA3 knockout mice and primary human umbilical vein endothelial cells (HUVECs). The absence of ANXA3 exacerbated sepsis outcomes, including increased mortality, lung injury, leukocyte infiltration, and vascular permeability. ANXA3 is highly expressed in endothelial cells and its loss results in the formation of cytoskeletal stress fibers and a decrease in the expression of the junction proteins zonula occludens (Zo)-1, vascular endothelial (VE)-cadherin, and claudin 5, leading to increase permeability. ANXA3 knockdown also upregulates E-selectin (CD62E) expression through the phosphorylation of activating transcription factor 2 (ATF2), which increases monocyte adhesion in HUVECs after LPS stimulation. Inhibiting actin polymerization reverse these effects. Thus, ANXA3 stabilizes the actin cytoskeleton, playing a protective role in endothelial dysfunction during sepsis.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2416904"},"PeriodicalIF":14.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprinted Patient-Derived Organoid Arrays Capture Intrinsic and Extrinsic Tumor Features for Advanced Personalized Medicine.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-28 DOI: 10.1002/advs.202407871

Jonghyeuk Han, Hye-Jin Jeong, Jeonghan Choi, Hyeonseo Kim, Taejoon Kwon, Kyungjae Myung, Kyemyung Park, Jung In Park, Samuel Sánchez, Deok-Beom Jung, Chang Sik Yu, In Ho Song, Jin-Hyung Shim, Seung-Jae Myung, Hyun-Wook Kang, Tae-Eun Park

{"title":"Bioprinted Patient-Derived Organoid Arrays Capture Intrinsic and Extrinsic Tumor Features for Advanced Personalized Medicine.","authors":"Jonghyeuk Han, Hye-Jin Jeong, Jeonghan Choi, Hyeonseo Kim, Taejoon Kwon, Kyungjae Myung, Kyemyung Park, Jung In Park, Samuel Sánchez, Deok-Beom Jung, Chang Sik Yu, In Ho Song, Jin-Hyung Shim, Seung-Jae Myung, Hyun-Wook Kang, Tae-Eun Park","doi":"10.1002/advs.202407871","DOIUrl":"https://doi.org/10.1002/advs.202407871","url":null,"abstract":"Heterogeneity and the absence of a tumor microenvironment (TME) in traditional patient-derived organoid (PDO) cultures limit their effectiveness for clinical use. Here, Embedded Bioprinting-enabled Arrayed PDOs (Eba-PDOs) featuring uniformly arrayed colorectal cancer (CRC) PDOs within a recreated TME is presented. This model faithfully reproduces critical TME attributes, including elevated matrix stiffness (≈7.5 kPa) and hypoxic conditions found in CRC. Transcriptomic and immunofluorescence microscopy analysis reveal that Eba-PDOs more accurately represent actual tissues compared to traditional PDOs. Furthermore, Eba-PDO effectively capture the variability of CEACAM5 expression-a critical CRC marker-across different patients, correlating with patient classification and differential responses to 5-fluorouracil treatment. This method achieves an uniform size and shape within PDOs from the same patient while preserving distinct morphological features among those from different individuals. These features of Eba-PDO enable the efficient development of a label-free, morphology-based predictive model using supervised learning, enhancing its suitability for clinical applications. Thus, this approach to PDO bioprinting is a promising tool for generating personalized tumor models and advancing precision medicine.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2407871"},"PeriodicalIF":14.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Insights into Heavy Metal Sequestration Through Metal-Phenolic Network-Confined Nano-HFO: Overlooking Iron Utilization and Modulating Electron Density.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-28 DOI: 10.1002/advs.202417798

Manyu Zhang, Xiaolin Du, Zhanqi Liu, Yujia Yang, Shuo Wang, Ningyi Chen, Yulin Wang, Yaran Song, Keju Sun, Qingrui Zhang

{"title":"New Insights into Heavy Metal Sequestration Through Metal-Phenolic Network-Confined Nano-HFO: Overlooking Iron Utilization and Modulating Electron Density.","authors":"Manyu Zhang, Xiaolin Du, Zhanqi Liu, Yujia Yang, Shuo Wang, Ningyi Chen, Yulin Wang, Yaran Song, Keju Sun, Qingrui Zhang","doi":"10.1002/advs.202417798","DOIUrl":"https://doi.org/10.1002/advs.202417798","url":null,"abstract":"Reducing toxic metal concentrations to trace levels remains a critical challenge in water remediation, largely due to the underutilization of hydrous ferric oxide (HFO), particularly within its inner layers. Herein, we present a novel strategy to enhance HFO utilization by in situ confinement of nano-HFO within polystyrene beads using a tannic acid-zirconium (TA-Zr) metalphenolic network, forming PS-Fe@TA-Zr. The TAZr network generates a highly negative microenvironment with tunable electron density at oxygen sites, facilitating Pb(II) enrichment and activating inner-layer Fe sites. Depth-profiling reveals a significant increase in the Pb/Fe ratio from 7.6% at the surface to 18.8% at 10 nm depth, highlighting the contribution of previously inaccessible active sites. The TAZr confinement also modulates electron density at Fe and O sites, enabling stronger hybridization with Pb 4f orbitals and enhancing Pb(II)HFO interactions. Compared with PS-Fe, PS-Fe@TA-Zr exhibits over 8-fold higher selectivity (Kd = 15,278 mL g-1), 5-fold faster kinetics, and can treat up to 1,680 L kg-1 with effective regeneration across six cycles in actual industrial wastewater. This work provides new insights into metalphenolic network-assisted design of nanocomposites for highly efficient iron utilization in heavy metal removal.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2417798"},"PeriodicalIF":14.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0