Advanced Science最新文献_第8页

The Lung Microbiome Modulates Pain-Like Behavior Via the Lung-Brain Axis in a Nitroglycerin-Induced Chronic Migraine Mouse Model.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202416348

Biying Liu, Chengya Huang, Xin Li, Haonan Yu, Yuefeng Xia, Kun Liu, Xingji You, Jingxiang Wu

{"title":"The Lung Microbiome Modulates Pain-Like Behavior Via the Lung-Brain Axis in a Nitroglycerin-Induced Chronic Migraine Mouse Model.","authors":"Biying Liu, Chengya Huang, Xin Li, Haonan Yu, Yuefeng Xia, Kun Liu, Xingji You, Jingxiang Wu","doi":"10.1002/advs.202416348","DOIUrl":"https://doi.org/10.1002/advs.202416348","url":null,"abstract":"Chronic migraine is one of the most common pain disorders, characterized by significant disability and a lack of safe, long-term, and effective treatment options. Recent studies highlight the interaction between the lung microbiota and the central nervous system. In this study, a nitroglycerin (NTG)-induced chronic migraine model is constructed in male C57BL/6 mice to explore these interactions. Notable alterations are observed in the lung microbiota of migraine-afflicted mice. Notably, there is a marked decrease in Proteobacteria in the chronic migraine group, associated with short-chain fatty acids and 5-hydroxytryptamine (5-HT). After the intratracheal injection of neomycin, the diversity of the lung microbiota is altered, resulting in the relief of migraines. This effect is also observed in mice that receive neomycin-treated bronchoalveolar lavage fluid (BALF) transplantation, further demonstrating the role of lung microbiota in this process. The altered lung microbiota activate the pulmonary vagus nerve via the Brain-derived neurotrophic factor-tropomyosin receptor kinase B (BDNF-TrkB) pathway in the lung, which projects to the central nucleus of the solitary tract (NTS) and the dorsal raphe nucleus (DRN). This activation, in turn, stimulates the 5-HT neurons in the DRN, resulting in increased serotonin levels that contribute to pain relief in the chronic migraine model.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2416348"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoparticle Delivery of Antisense miR162 Inhibits Invasive Habitat Adaption of Alternanthera Philoxeroides.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202416747

Qianqian Hu, Erfeng Kou, Xiuzhen Liao, Ruiyi Qiu, Qi Tang, Huan Zhang, Yun Zheng, Ji Yang, Binglian Zheng

{"title":"Nanoparticle Delivery of Antisense miR162 Inhibits Invasive Habitat Adaption of Alternanthera Philoxeroides.","authors":"Qianqian Hu, Erfeng Kou, Xiuzhen Liao, Ruiyi Qiu, Qi Tang, Huan Zhang, Yun Zheng, Ji Yang, Binglian Zheng","doi":"10.1002/advs.202416747","DOIUrl":"https://doi.org/10.1002/advs.202416747","url":null,"abstract":"Phenotypic flexibility in adaptive traits is crucial for organisms to thrive in changing environments. Alternanthera philoxeroides, native to South America, has become an invasive weed in Asia. The mechanism by which invasive capacity is achieved remains unknown. Here, it is demonstrated that miR162 plays a crucial role in submergence survival for A. philoxeroides. These results highlight that the level of miR162 significantly increases in stems from 3 to 48 h upon water submergence, and knockdown of miR162 via TRV-based VIGS system significantly disrupts stem elongation upon water submergence, ultimately resulting in a failure of plants protruding from the water surface. Interestingly, miR162 is not up-regulated in the noninvasive congeneric alien species Alternanthera pungens, which is also native to South America but has retained its original habitats in Asia. The presence of anaerobic responsive elements (AREs) in the promoter sequences of MIR162 from A. philoxeroides rather than A. pungens may contribute to its invasion capacity. Importantly, nanoparticle delivery of antisense RNA oligonucleotides of miR162 significantly impairs stem elongation during water submergence. Thus, our findings reveal that the achievement of specific miRNA activity can drive rapid phenotypic variation, and miR162 has the potential as a bio-pesticide for controlling the invasive growth of A. philoxeroides.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2416747"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling Surface Species Formed on Ni-Fe Spinel Oxides During the Oxygen Evolution Reaction at the Atomic Scale.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202501967

Weikai Xiang, Sheila Hernandez, Pouya Hosseini, Fan Bai, Ulrich Hagemann, Markus Heidelmann, Tong Li

{"title":"Unveiling Surface Species Formed on Ni-Fe Spinel Oxides During the Oxygen Evolution Reaction at the Atomic Scale.","authors":"Weikai Xiang, Sheila Hernandez, Pouya Hosseini, Fan Bai, Ulrich Hagemann, Markus Heidelmann, Tong Li","doi":"10.1002/advs.202501967","DOIUrl":"https://doi.org/10.1002/advs.202501967","url":null,"abstract":"Optimizing electrocatalyst performance requires an atomic-scale understanding of surface state changes and how those changes affect activity and stability during the reaction. This is particularly important for the oxygen evolution reaction (OER) since the electrocatalytically active surfaces undergo substantial reconstruction and transformation. Herein, a multimodal method is employed that combines X-ray photoemission spectroscopy, transmission electron microscopy, atom probe tomography, operando surface-enhanced Raman spectroscopy with electrochemical measurements to examine the surface species formed on NiFe2O4, P-doped NiFe2O4 and Ni1.5Fe1.5O4 upon OER cycling. The activated NiFe2O4 and P-doped NiFe2O4 exhibit a significantly lower Tafel slope (≈40 mV dec-1) than Ni1.5Fe1.5O4 (≈90 mV dec-1), although oxyhydroxides are grown on all three Ni-Fe spinels during OER. This is likely attributed to the formation of a ≈1 nm highly defective layer with a higher oxygen concentration on the activated NiFe2O4 and P-doped NiFe2O4 nanoparticle surfaces (than that in bulk), which improves the charge transfer kinetics toward OER. Such surface species are not formed on Ni1.5Fe1.5O4. Overall, this study provides a mechanistic understanding of the role of Fe, P, and Ni in forming active oxygen species in the Ni-based spinels toward OER.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2501967"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PPY-Induced iCAFs Cultivate an Immunosuppressive Microenvironment in Pancreatic Cancer.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202413432

Mengdie Cao, Wang Peng, Bin Cheng, Ronghua Wang, Wei Chen, Luyao Liu, Hai Huang, Shiru Chen, Haochen Cui, JingWen Liang, Qiaodan Zhou, Si Xiong, Shuya Bai, Luoxia Liu, Yuchong Zhao

{"title":"PPY-Induced iCAFs Cultivate an Immunosuppressive Microenvironment in Pancreatic Cancer.","authors":"Mengdie Cao, Wang Peng, Bin Cheng, Ronghua Wang, Wei Chen, Luyao Liu, Hai Huang, Shiru Chen, Haochen Cui, JingWen Liang, Qiaodan Zhou, Si Xiong, Shuya Bai, Luoxia Liu, Yuchong Zhao","doi":"10.1002/advs.202413432","DOIUrl":"https://doi.org/10.1002/advs.202413432","url":null,"abstract":"Pancreatic ductal adenocarcinoma (PDAC) is characterized by cancer cells surrounded by affluent stromal components, which may underlie their limited response to various therapeutic interventions, including immunotherapy. Inflammatory cancer-associated fibroblasts (iCAFs), a crucial subset of CAFs within the PDAC microenvironment, play a pivotal role in shaping an immunosuppressive microenvironment. In this study, single-cell RNA sequencing analysis is performed to screen for cancer cells-secreted proteins associated with iCAF induction, and PPY (pancreatic polypeptide) is validated as a potent inducer. Unlike previously reported iCAF inducers, PPY is a gastrointestinal hormone predominantly expressed in the pancreas, suggesting that targeting it may have minimal systemic effects. Multiplex immunohistochemistry (mIHC) on human PDAC tissue microarrays, orthotopic allograft mouse models, and co-culture experiments are utilized to validate the crucial role of PPY in iCAF induction. Mechanistic studies integrating mRNA sequencing, immunoprecipitation-mass spectrometry, and molecular docking reveal that PPY induces iCAFs by activating the non-canonical NF-κB pathway through EGFR. Importantly, targeting PPY enhanced the efficacy of anti-PD-1 immunotherapy in KPC (KrasLSL-G12D/+; Trp53LSL-R172H/+; Pdx1-Cre) mice, as evidenced by reduced tumor burden on PET-CT imaging and improved survival. This research is expected to provide a novel strategy for improving immunotherapy in PDAC by targeting a key inducer of iCAFs.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2413432"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Longitudinal, Multimodal Tracking Reveals Lasting Neurovascular Impact of Individual Microinfarcts.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202417003

Yifu Jin, Fei He, Haad Rathore, Yingchu Sun, Jia-Ao Zhang, Xinyu Li, Rongkang Yin, Hanlin Zhu, Chong Xie, Lan Luan

{"title":"Longitudinal, Multimodal Tracking Reveals Lasting Neurovascular Impact of Individual Microinfarcts.","authors":"Yifu Jin, Fei He, Haad Rathore, Yingchu Sun, Jia-Ao Zhang, Xinyu Li, Rongkang Yin, Hanlin Zhu, Chong Xie, Lan Luan","doi":"10.1002/advs.202417003","DOIUrl":"https://doi.org/10.1002/advs.202417003","url":null,"abstract":"Microinfarcts, the \"invisible lesions\", are prevalent in aged and injured brains and associated with cognitive impairments, yet their neurophysiological impact remains largely unknown. Using a multimodal chronic neural platform that combines functional microvasculature imaging with spatially resolved neural recording, the neurovascular effect of a single microinfarct is investigated. Unlike larger strokes, microinfarcts induced only temporary suppression of neural activity with minimal cell death, with recovery paralleling vasculature remodeling at the infarct core. Neural activity is more severely suppressed at the shallower cortical layer despite milder vascular damage compared to deeper layers, and the excitability of fast-spiking interneurons attenuation is accompanied by heightened bursting of regular spiking neurons. Spike phase locking at the low-gamma band is disrupted, indicating a lasting impairment of long-range assembly communication. These results highlight the subtle yet significant neurovascular disruptions of a single microinfarct.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2417003"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Transition Metal-Free Approach for the Conversion of Real-Life Cellulose-Based Biomass into Formate.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202415339

Tong Zhang, Peng Ren, Yuman Qin, Thanh Huyen Vuong, Ana V Cunha, Remco W A Havenith, Jabor Rabeah, Shoubhik Das

{"title":"A Transition Metal-Free Approach for the Conversion of Real-Life Cellulose-Based Biomass into Formate.","authors":"Tong Zhang, Peng Ren, Yuman Qin, Thanh Huyen Vuong, Ana V Cunha, Remco W A Havenith, Jabor Rabeah, Shoubhik Das","doi":"10.1002/advs.202415339","DOIUrl":"https://doi.org/10.1002/advs.202415339","url":null,"abstract":"Formic acid (FA) and its salt are recognized as valuable molecules for various industries such as textiles and pharmaceuticals. Currently, the global demand of FA and its salts stands at 1.137 million metric tons per year, necessitating the development of sustainable methods to meet the future demands. While numerous approaches are developed for the generation of FA but the requirement of harsh reaction conditions to achieve them is unavoidable. On the other hand, the world production of biomass is estimated at 146 billion metric tons per year and that can be considered as a prospective source of FA and their salts. Additionally, cellulose accounts for approximately 35-45% of the biomass composition. Considering this, a visible-light-mediated approach is presented to produce formate directly from biomass at room temperature as well as at atmospheric pressure. In this approach, selective generation of hydroxyl radical has been achieved which later converted sugars, cellulose, and hemicellulose into formate. Furthermore, the conversion of cellulose-rich daily-life materials such as discarded paper into the product through a series of flow experiments is demonstrated. Finally, mechanistic investigations including electron paramagnetic resonance (EPR) spectroscopy, and density functional theory (DFT) calculations are conducted to gain insights into the underlying reaction mechanism.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2415339"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Graphene Nanofibers by Integrated Manufacturing of Electrospinning and Laser Graphitization for Miniaturized Energy Storage Devices.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202414607

Bumjun Park, Shirin Movaghgharnezhad, Seung Min Lee, Yonghyeon Park, Sejin Son, Yun Suk Huh, Pilgyu Kang

{"title":"Graphene Nanofibers by Integrated Manufacturing of Electrospinning and Laser Graphitization for Miniaturized Energy Storage Devices.","authors":"Bumjun Park, Shirin Movaghgharnezhad, Seung Min Lee, Yonghyeon Park, Sejin Son, Yun Suk Huh, Pilgyu Kang","doi":"10.1002/advs.202414607","DOIUrl":"https://doi.org/10.1002/advs.202414607","url":null,"abstract":"Carbon nanofibers (CNFs) are emerging as promising materials for miniaturized energy storage devices (MESDs) due to their high specific surface area, excellent electrochemical performance, low internal resistance, and durability. Their versatility and tunability make them ideal candidates for various applications, making CNFs a key player in advancing compact and efficient energy storage solutions. Nonetheless, CNFs necessitate an extra step involving either physical or chemical treatments to regulate their morphology, augment surface area, and create micropatterns suitable for MESDs. Here, innovations in material fabrication using an integrated manufacturing process are reported that combines electrospinning and laser-induced graphitization to create graphene nanofibers (GNFs) from fluorinated polyimide nanofibers (fPI NFs). Initially, electrospinning yields uniformly sized and shaped fluorinated poly(amic) acid nanofibers, which are subsequently thermally imidized to form fPI NFs. Laser photothermal treatment of fPI NFs generates hierarchical meso- and nanopores in GNFs, enhancing specific surface area and electrochemical properties, including specific capacitance, cyclic stability, rate capability, areal capacitance, power density, and energy density. This integrated approach synergistically fabricates GNFs for MESD applications, particularly GNF-based micro-supercapacitors (MSCs), demonstrating a remarkable areal capacitance and an aerial energy density two orders of magnitude higher than MSCs based on laser-induced graphene derived from conventional polyimide film.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2414607"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asymmetric Inter-Hemisphere Communication Contributes to Speech Acquisition of Toddlers with Cochlear Implants.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202309194

Xue Zhao, Meiyun Wu, Haotian Liu, Yuyang Wang, Zhikai Zhang, Yuhe Liu, Yu-Xuan Zhang

{"title":"Asymmetric Inter-Hemisphere Communication Contributes to Speech Acquisition of Toddlers with Cochlear Implants.","authors":"Xue Zhao, Meiyun Wu, Haotian Liu, Yuyang Wang, Zhikai Zhang, Yuhe Liu, Yu-Xuan Zhang","doi":"10.1002/advs.202309194","DOIUrl":"https://doi.org/10.1002/advs.202309194","url":null,"abstract":"How the lateralized language network and its functions emerge with early auditory experiences remains largely unknown. Here, early auditory development is examined using repeated optical imaging for cochlear implanted (CI) toddlers with congenital deafness from onset of restored hearing to around one year of CI hearing experiences. Machine learning models are constructed to resolve how functional organization of the bilateral language network and its sound processing support the CI children's post-implantation development of auditory and verbal communication skills. Behavioral improvement is predictable by cortical processing as well as by network organization changes, with the highest classification accuracy of 81.57%. For cortical processing, behavioral prediction is better for the left than the right hemisphere and for speech than non-speech processing. For network organization, the best prediction is obtained for resting state, with greater contribution from inter-hemisphere connections between non-homologous regions than from within-hemisphere connections. Most interestingly, systematic connectivity-to-activity models reveal that speech processing of the left language network is developmentally supported largely by global network organization, particularly asymmetric inter-hemisphere communication, rather than functional segregation of local network. These findings collectively confirm the importance of asymmetric inter-hemisphere communication in formation of the lateralized language network and its functional development with early auditory experiences.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2309194"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protease-Mediated Synthesis of Zein Nanofibrils: From Structural Elucidation to Functional Application.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202414606

Mingqin Li, Tonghui Jin, Simone Wüthrich, Jiangtao Zhou, Qiyao Sun, Ting Li, Zhou Dong, Eva Maria Zunzuneigui Bru, Raffaele Mezzenga

{"title":"Protease-Mediated Synthesis of Zein Nanofibrils: From Structural Elucidation to Functional Application.","authors":"Mingqin Li, Tonghui Jin, Simone Wüthrich, Jiangtao Zhou, Qiyao Sun, Ting Li, Zhou Dong, Eva Maria Zunzuneigui Bru, Raffaele Mezzenga","doi":"10.1002/advs.202414606","DOIUrl":"https://doi.org/10.1002/advs.202414606","url":null,"abstract":"The fibrillization of plant-based proteins enhances their functionality, enabling potential applications in food and sustainable materials. Zein, a highly hydrophobic protein from corn, is a versatile industrial ingredient, but its functionality is limited to environments containing high levels of organic solvents. This study aims to develop a protease-assisted approach for synthesizing zein nanofibrils as functional building blocks, eliminating the need for organic solvents in the conventional process. Through proteomics, microscopy, and spectroscopy, the bioprocess and structural features of these novel nanofibrils are characterized. The results reveal that over 50% of α-zein sequence is prone to fibrillization, with pepsin demonstrating a clear advantage in efficiently releasing fibrillization-prone peptide segments (bioconversion > 70%) and producing a peptide mixture suitable for self-assembly. The fibrillization process is significantly enhanced by increasing peptide concentration and adding the anionic surfactant sodium dodecyl sulfate, which can lead to the formation of semiflexible fibrils with amyloid-like β-sheet structures. These nanofibrils outperformed native zein as emulsifiers in high internal phase emulsions and are able to form fibrous hydrogels. The protease-assisted fibrillization process achieved in this study provides an effective solution for expanding applications of zein or corn proteins in a purely aqueous environment.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2414606"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep Learning-Enhanced Hand-Driven Microfluidic Chip for Multiplexed Nucleic Acid Detection Based on RPA/CRISPR.

IF 14.3 1区材料科学

Advanced Science Pub Date : 2025-03-31 DOI: 10.1002/advs.202414918

Tao Xu, Ying Zhang, Shunji Li, Chenxi Dai, Hongguo Wei, Dongjuan Chen, Yunjun Zhao, He Liu, Deliang Li, Peng Chen, Bi-Feng Liu, Ye Tian

{"title":"Deep Learning-Enhanced Hand-Driven Microfluidic Chip for Multiplexed Nucleic Acid Detection Based on RPA/CRISPR.","authors":"Tao Xu, Ying Zhang, Shunji Li, Chenxi Dai, Hongguo Wei, Dongjuan Chen, Yunjun Zhao, He Liu, Deliang Li, Peng Chen, Bi-Feng Liu, Ye Tian","doi":"10.1002/advs.202414918","DOIUrl":"https://doi.org/10.1002/advs.202414918","url":null,"abstract":"The early detection of high-risk human papillomavirus (HR-HPV) is crucial for the assessment and improvement of prognosis in cervical cancer. However, existing PCR-based screening methods suffer from inadequate accessibility, which dampens the enthusiasm for screening among grassroots populations, especially in resource-limited areas, and contributes to the persistently high mortality rate of cervical cancer. Here, a portable system is proposed for multiplexed nucleic acid detection, termed R-CHIP, that integrates Recombinase polymerase amplification (RPA), CRISPR detection, Hand-driven microfluidics, and an artificial Intelligence Platform. The system can go from sample pre-processing to results readout in less than an hour with simple manual operation. Optimized for sensitivity of 10-17 M for HPV-16 and 10-18 M for HPV-18, R-CHIP has an accuracy of over 95% in 300 tests on clinical samples. In addition, a smartphone microimaging system combined with the ResNet-18 deep learning model is used to improve the readout efficiency and convenience of the detection system, with initial prediction accuracies of 96.0% and 98.0% for HPV-16 and HPV-18, respectively. R-CHIP, as a user-friendly and intelligent detection platform, has great potential for community-level HR-HPV screening in resource-constrained settings, and contributes to the prevention and early diagnosis of other diseases.","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e2414918"},"PeriodicalIF":14.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0