Environmental Toxicology and Chemistry最新文献

{"title":"Enhancing ecological risk assessment of chemicals for terrestrial ecosystems through ecosystem services approach.","authors":"Hamzat O Fajana, Eric G Lamb, Steven D Siciliano","doi":"10.1093/etojnl/vgae080","DOIUrl":"https://doi.org/10.1093/etojnl/vgae080","url":null,"abstract":"","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute-to-chronic ratios of aquatic phototoxicity of polycyclic aromatic hydrocarbons and the prediction of chronic phototoxicity using the phototoxic target lipid model.","authors":"Angeline Tillmanns, Solmaz Marzooghi, Christopher J Kennedy","doi":"10.1093/etojnl/vgae069","DOIUrl":"https://doi.org/10.1093/etojnl/vgae069","url":null,"abstract":"<p><p>Polycyclic aromatic hydrocarbons (PAHs) are a group of organic compounds that can be toxic to aquatic life at environmentally relevant concentrations. The toxicity of some PAHs increases in the presence of ultraviolet radiation (UVR). The phototoxic target lipid model (PTLM) builds on the narcotic target lipid model and predicts acute phototoxicity by accounting for the light absorbed by PAHs. Given the lack of chronic data for PAHs exposed to UVR, an acute-to-chronic ratio (ACR) was proposed as a means for extending this model for predicting chronic toxicity. No information could be found on ACRs derived for phototoxic chemicals and therefore, this study had two objectives: (a) to generate acute and chronic toxicity data with and without exposure to UVR; and (b) to test the ability of the PTLM to predict chronic toxicity using an ACR. Acute and chronic bioassays were conducted on eight species, with each exposed to three PAHs and two light treatments: with and without UVR. Although there was a general trend of higher ACRs in UVR treatments, there was no statistical difference across all species in the ACR values for UVR treatments. Data generated herein were combined with data collated through a literature search to generate a global ACR of 11.6. Twenty of the 24 predicted phototoxic effect concentration affecting 10% of species (EC10) values calculated using the PTLM and ACR were within a factor of 10 of the observed phototoxic EC10 or lethal concentration affecting 5% of species values. This study demonstrates the capability of ACR to be applied in the context of phototoxicity and therefore proposes an unprecedented methodology to predict the chronic phototoxicity of PAHs.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational new approach methods guide focused testing and enhance understanding of chlorantraniliprole toxicity across species.","authors":"Marissa A Jensen-Brickley, Leah Glimsdal, Abigail Johnson, Emma Stacy, Kelvin Santana-Rodriguez, Kali Mattingly, Daniel L Villeneuve, Russ Hockett, Brett Blackwell, Jenna Cavallin, Carlie A LaLone","doi":"10.1093/etojnl/vgae057","DOIUrl":"https://doi.org/10.1093/etojnl/vgae057","url":null,"abstract":"<p><p>Diamide insecticides, specifically chlorantraniliprole (CHL), have been rising in popularity over the past decade, becoming one of the most widely used insecticide classes globally. These insecticides target the ryanodine receptor (RyR), primarily for control of lepidopteran agricultural pests. Field studies have revealed that some lepidopteran species have developed mutations where a methionine in a particular position (e.g., I4790M) increases resistance to CHL. The toxicity data for CHL across species is limited, as is the case for many chemicals, which creates an opportunity to apply both traditional toxicity test methods and new approach methods (NAMs) to address data gaps. Here, the U.S. Environmental Protection Agency's Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool was used to query the RyR to generate susceptibility predictions for species exposed to CHL to fill those data gaps. These SeqAPASS results generated testable hypotheses that were used to guide focused acute aquatic toxicity studies using Daphnia magna, Daphnia pulex, Pimephales promelas, and Danio rerio. The fish species were not sensitive to CHL, whereas D. magna and D. pulex were found to be sensitive to CHL at environmentally relevant concentrations, despite having the methionine residue in the position of the I4790M resistance mutation. Additional SeqAPASS results showed that many other species, including beneficial pollinators and Lepidoptera, are predicted as likely susceptible to CHL. This study provided multiple lines of evidence toward the unlikelihood for the I4790M mutation to be the primary cause of resistance across species, filled knowledge gaps concerning CHL toxicity across species, and generated predictions of susceptibility for nontarget species that are not generally amenable to toxicity testing. This work presents a case example that demonstrates how NAMs can be used in combination with other types of data to direct targeted testing and build confidence in predictive approaches for their use in risk assessment.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicogenomic signatures of estrogen-related modes of action in the zebrafish embryo.","authors":"Milena Frelih, Steve U Ayobahan, Fatma Marghany, Fabian Essfeld, Sebastian Eilebrecht","doi":"10.1093/etojnl/vgae059","DOIUrl":"https://doi.org/10.1093/etojnl/vgae059","url":null,"abstract":"<p><p>Endocrine disruptors represent a diverse array of chemicals known to interfere with the endocrine systems of both human and environmental organisms, adversely affecting reproduction, development, and behavior, thus raising significant health and ecological concerns. Traditional regulatory tests for endocrine activity typically involve juvenile or adult fish, which is both time-consuming and resource-intensive and necessitates substantial animal use. This study adopts a transcriptomic approach to identify toxicogenomic signatures associated with the disruption of estrogen signaling in zebrafish (Danio rerio) embryos. Utilizing a modified zebrafish embryo toxicity test based on Organisation for Economic Co-operation and Development test guideline 236, the embryos were exposed to two sublethal concentrations of estradiol, bisphenol A, and fulvestrant. Despite no significant effects on survival or hatching rate observed in treated groups compared with the controls, our study effectively pinpointed several genes, including vtg1, cyp19a1b, fam20cl, sult1st2, pck1, agxtb, hsd17b12a, ptgs2a, and ccn1, as linked to a disruption of estrogen signaling. These genes emerge as promising biomarker candidates for identifying and distinguishing estrogen-related modes of action. Additionally, this approach not only supports the detection of potential endocrine disruptors but also opens up possibilities for prioritizing substances for higher tier endocrine testing, which could substantially reduce animal testing in the future.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Observational studies on per- and polyfluoroalkyl substance toxicity in marine species: hints for the extrapolation of a screening value.","authors":"Roberto Miniero, Gianfranco Brambilla, Fulvio Maffucci, Sandra Hochscheid, Mauro Esposito","doi":"10.1093/etojnl/vgae065","DOIUrl":"https://doi.org/10.1093/etojnl/vgae065","url":null,"abstract":"<p><p>A statistical procedure has been developed to derive a screening value from an observational study related to the developmental toxicity observed in loggerhead turtle (Caretta caretta) eggs exposed to long chain per- and polyfluoroalkyl substances (PFAS). A dataset of 41 nests in which the hatching rate was inversely correlated with the increase in the PFAS concentration in unhatched eggs was processed via a categorical regression approach. After outliers identification and removal, categorical regression analysis tested the relationships of the outcomes with the following parameters: perfluoro-nonanoic (PFNA), decanoic (PFDA), undecanoic (PFUdA), and dodecanoic (PFDoA) acids; perfluoroctansulfonate (PFOS); polychlorobiphenyls (PCBs) 28, 52, 101, 138, 153, 180; lead (Pb), total mercury (Hgtot), and cadmium (Cd); and other factors, such as \"nest site,\" \"clutch size,\" \"incubation duration,\" and \"nest minimum depth,\" as confounders/modifiers of the hatching rate. Among considered contaminants, PFOS, PFDA, and PFNA only were significant (p ˂ 0.05), as were \"nest site,\" \"clutch size,\" and \"incubation duration,\" confirming their possible role in decreasing the hatching rate of sea turtle eggs. According to a chemical-specific visual strategy, PFOS only showed a typical monotonic dose/response curve, which allowed the identification of provisional hypothetical thresholds of PFOS, 1,386 (CI95 = 1,080-1,692) ng/kg, corresponding to average hatching rates of 93.3% (CI95 = 91.4%-95.2%). Our preliminary results indicate the feasibility of the extrapolation of a screening value from observational studies under the following requirements: (a) individuation of most influencing factors for the outcome; (b) datasets referred to baseline contamination; (c) selection of undisturbed nests; (d) appropriate statistical multivariate methodology. This work aims to contribute to the New Approach Methods (NAMs) to assess PFAS early-stage embriotoxicity in marine biota.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing the quantitative understanding of adverse outcome pathways: current status, methodologies, and future directions.","authors":"Jaeseong Jeong, Manvel Gasparyan, Jinhee Choi","doi":"10.1093/etojnl/vgae063","DOIUrl":"https://doi.org/10.1093/etojnl/vgae063","url":null,"abstract":"<p><p>An adverse outcome pathway (AOP) framework maps the sequence of events leading to adverse outcomes from chemical exposures, providing a mechanistic understanding often absent in traditional methods. The quantitative AOP (qAOP) advances AOP by integrating quantitative data and mathematical modeling, thereby providing a more precise comprehension of relationships between molecular initiating events, key events, and adverse outcomes. This review critically examines three primary methodologies: systems toxicology, regression modeling, and Bayesian network modeling, highlighting their strengths, limitations, and specific data requirements within toxicology. Through an analysis of current methodologies and challenges, this review emphasizes the integration of experimental and computational approaches to elucidate key event relationships and proposes strategies for overcoming limitations through standardized protocols and advanced computational tools. By outlining future research directions and the potential of qAOPs to transform chemical risk assessment, this review aims to contribute to the advancement of regulatory science and the protection of public health and the environment.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative glucocorticoid receptor agonism: In silico, in vitro, and in vivo and identification of potential biomarkers for synthetic glucocorticoid exposure.","authors":"Alexander R Cole, Brett R Blackwell, Jenna E Cavallin, Jacob E Collins, Ashley R Kittelson, Yesmeena M Shmaitelly, Laura M Langan, Daniel L Villeneuve, Bryan W Brooks","doi":"10.1093/etojnl/vgae041","DOIUrl":"https://doi.org/10.1093/etojnl/vgae041","url":null,"abstract":"<p><p>The glucocorticoid receptor (GR) is present in almost every vertebrate cell and is utilized in many biological processes. Despite an abundance of mammalian data, the structural conservation of the receptor and cross-species susceptibility, particularly for aquatic species, has not been well defined. Efforts to reduce, refine, and/or replace animal testing have increased, driving the impetus to advance development of new approach methodologies (NAMs). Here we used in silico, in vitro, and in vivo methods to elucidate a greater understanding of receptor-mediated effects of synthetic glucocorticoid exposure in teleost fish. Evolutionary conservation of amino acid residues critical for transcriptional activation was confirmed in silico using sequence alignment to predict across species susceptibility. Subsequent in vitro assays using zebrafish and human GR provided evidence of physiological congruence of GR agonism. Finally, adult fathead minnows (Pimephales promelas) were exposed in vivo to the synthetic glucocorticoids, dexamethasone (0.04, 400, 4,000 µg/L) and beclomethasone dipropionate (130 µg/L), and GR agonism confirmed via digital polymerase chain reaction; in addition, EcoToxChip analyses identified potential mRNA biomarkers following glucocorticoid exposure. These findings support the use of NAMs to potentially reduce multispecies in vivo experimentation while providing empirical evidence that expands the taxonomic domain of applicability for the GR agonism molecular initiating event within the broader GR agonism adverse outcome pathway network.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a bioavailability-based acute effects assessment method for nickel.","authors":"Charlotte Nys, Elizabeth Middleton, Emily Garman, Chris Schlekat, Patrick Van Sprang, Karel De Schamphelaere","doi":"10.1093/etojnl/vgae071","DOIUrl":"https://doi.org/10.1093/etojnl/vgae071","url":null,"abstract":"<p><p>This study aimed to develop a bioavailability-based effects assessment method for nickel (Ni) to derive acute freshwater environmental thresholds in Europe. The authors established a reliable acute freshwater Ni ecotoxicity database covering 63 different freshwater species, and the existing acute Ni bioavailability models for invertebrates were revised. A single average invertebrate bioavailability model was proposed, in which the protective effects of Ca2+ and Mg2+ on Ni2+ toxicity were integrated as a single-site competition effect at the Ni biotic ligand. The biotic ligand stability constants for Ca2+ and Mg2+ (log KCaBL = 3.80 and log KMgBL = 3.32) were derived by averaging these parameters from three existing cladoceran models. A pH extension was also integrated into the average invertebrate bioavailability model to reflect the increase in free Ni2+ toxicity observed greater than pH 8.0. The proposed invertebrate model has further been validated using an extensive dataset of acute toxicity data covering 15 different invertebrate species. Evaluating the extrapolation of the invertebrate model to plant species revealed significant uncertainty about the applicability of the acute Ni bioavailability models for plants. The newly developed acute invertebrate model was used alongside the existing acute fish and algae bioavailability models to support an acute bioavailability normalization approach for Ni. By combining these bioavailability models with the acute toxicity dataset for Ni, a normalized species sensitivity distribution approach is proposed to derive site-specific acute environmental thresholds, expressed by the HC5L(E)C50 (i.e., dissolved Ni concentration resulting in at least 50% effect for 5% of the species). The applicability ranges of the acute Ni bioavailability normalization approach are estimated to be valid for approximately 70% of European freshwaters. The proposed approach serves as a basis to incorporate bioavailability into the compliance evaluation relative to acute environmental threshold values for Ni in Europe.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactive effects of elevated temperature and venlafaxine on mitochondrial respiration and enzymatic capacity in Nile tilapia (Oreochromis niloticus).","authors":"Brittney G Borowiec, Karyn B Robichaud, Paul M Craig","doi":"10.1093/etojnl/vgae082","DOIUrl":"https://doi.org/10.1093/etojnl/vgae082","url":null,"abstract":"<p><p>Warming events are becoming more frequent and extreme in aquatic environments worldwide. Concurrently, many environments are polluted with biologically active compounds such as pharmaceuticals. Understanding how these challenges interact is critical for understanding the climate crisis, as contaminants may modulate how ectotherms respond to heat stress or vice versa. One potential site for these heat × contaminant interactions is the mitochondrion, which is central to metabolism, implicated in thermal tolerance, and evolutionarily conserved. Using high-resolution respirometry, we investigated how acute warming (to 35 °C, 40 °C, or 45 °C from 25 °C) impacted the respiration, coupling, and metabolic capacity of liver mitochondria isolated from Nile tilapia, and how exposure to environmentally relevant levels of the ubiquitous antidepressant venlafaxine modulated those effects. Mitochondria exposed to hotter temperatures had higher respiration rates and decreased respiratory control ratio compared to mitochondria exposed to cooler temperatures. The depressive effects of venlafaxine on respiration rates through complex I and II or complex II only (State 3 and State 4), as well as complex IV-linked respiration, were mild except in mitochondria exposed to high temperatures, suggesting an interactive effect of warming and contaminant exposure. Finally, we found that the maximal enzyme activity of intact mitochondria (represented by mitochondrial respiration) showed a different pattern of response to warming and venlafaxine compared to its underlying components (as reflected by the activity of succinate dehydrogenase [complex II] and cytochrome c oxidase [complex IV]), demonstrating the value of incorporating both interactive and reductive approaches in understanding how mitochondria cope with anthropogenic changes in the environment.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A combination of high-throughput in vitro and in silico new approach methods for ecotoxicology hazard assessment for fish.","authors":"Jo Nyffeler, Felix R Harris, Clinton Willis, Gabrielle Byrd, Brett Blackwell, Beate I Escher, Alex Kasparek, John Nichols, Jonathan T Haselman, Grace Patlewicz, Daniel L Villeneuve, Joshua A Harrill","doi":"10.1093/etojnl/vgae083","DOIUrl":"https://doi.org/10.1093/etojnl/vgae083","url":null,"abstract":"<p><p>Fish acute toxicity testing is used to inform environmental hazard assessment of chemicals. In silico and in vitro approaches have the potential to reduce the number of fish used in testing and increase the efficiency of generating data for assessing ecological hazards. Here, two in vitro bioactivity assays were adapted for use in high-throughput chemical screening. First, a miniaturized version of the Organisation for Economic Co-operation and Development (OECD) test guideline 249 plate reader-based acute toxicity assay in RTgill-W1 cells was developed. Second, the Cell Painting (CP) assay was adapted for use in RTgill-W1 cells along with an imaging-based cell viability assay. Then, 225 chemicals were tested in each assay. Potencies and bioactivity calls from the plate reader and imaging-based cell viability assays were comparable. The CP assay was more sensitive than either cell viability assay in that it detected a larger number of chemicals as bioactive, and phenotype altering concentrations (PACs) were lower than concentrations that decreased cell viability. An in vitro disposition (IVD) model that accounted for sorption of chemicals to plastic and cells over time was applied to predict freely dissolved PACs and compared with in vivo fish toxicity data. Adjustment of PACs using IVD modeling improved concordance of in vitro bioactivity and in vivo toxicity data. For the 65 chemicals where comparison of in vitro and in vivo values was possible, 59% of adjusted in vitro PACs were within one order of magnitude of in vivo toxicity lethal concentrations for 50% of test organisms. In vitro PACs were protective for 73% of chemicals. This combination of in vitro and in silico approaches has the potential to reduce or replace the use of fish for in vivo toxicity testing.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}