Environmental Toxicology and Chemistry最新文献

{"title":"Comparative glucocorticoid receptor agonism: In silico, in vitro, and in vivo and identification of potential biomarkers for synthetic glucocorticoid exposure.","authors":"Alexander R Cole, Brett R Blackwell, Jenna E Cavallin, Jacob E Collins, Ashley R Kittelson, Yesmeena M Shmaitelly, Laura M Langan, Daniel L Villeneuve, Bryan W Brooks","doi":"10.1093/etojnl/vgae041","DOIUrl":"https://doi.org/10.1093/etojnl/vgae041","url":null,"abstract":"<p><p>The glucocorticoid receptor (GR) is present in almost every vertebrate cell and is utilized in many biological processes. Despite an abundance of mammalian data, the structural conservation of the receptor and cross-species susceptibility, particularly for aquatic species, has not been well defined. Efforts to reduce, refine, and/or replace animal testing have increased, driving the impetus to advance development of new approach methodologies (NAMs). Here we used in silico, in vitro, and in vivo methods to elucidate a greater understanding of receptor-mediated effects of synthetic glucocorticoid exposure in teleost fish. Evolutionary conservation of amino acid residues critical for transcriptional activation was confirmed in silico using sequence alignment to predict across species susceptibility. Subsequent in vitro assays using zebrafish and human GR provided evidence of physiological congruence of GR agonism. Finally, adult fathead minnows (Pimephales promelas) were exposed in vivo to the synthetic glucocorticoids, dexamethasone (0.04, 400, 4,000 µg/L) and beclomethasone dipropionate (130 µg/L), and GR agonism confirmed via digital polymerase chain reaction; in addition, EcoToxChip analyses identified potential mRNA biomarkers following glucocorticoid exposure. These findings support the use of NAMs to potentially reduce multispecies in vivo experimentation while providing empirical evidence that expands the taxonomic domain of applicability for the GR agonism molecular initiating event within the broader GR agonism adverse outcome pathway network.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A combination of high-throughput in vitro and in silico new approach methods for ecotoxicology hazard assessment for fish.","authors":"Jo Nyffeler, Felix R Harris, Clinton Willis, Gabrielle Byrd, Brett Blackwell, Beate I Escher, Alex Kasparek, John Nichols, Jonathan T Haselman, Grace Patlewicz, Daniel L Villeneuve, Joshua A Harrill","doi":"10.1093/etojnl/vgae083","DOIUrl":"https://doi.org/10.1093/etojnl/vgae083","url":null,"abstract":"<p><p>Fish acute toxicity testing is used to inform environmental hazard assessment of chemicals. In silico and in vitro approaches have the potential to reduce the number of fish used in testing and increase the efficiency of generating data for assessing ecological hazards. Here, two in vitro bioactivity assays were adapted for use in high-throughput chemical screening. First, a miniaturized version of the Organisation for Economic Co-operation and Development (OECD) test guideline 249 plate reader-based acute toxicity assay in RTgill-W1 cells was developed. Second, the Cell Painting (CP) assay was adapted for use in RTgill-W1 cells along with an imaging-based cell viability assay. Then, 225 chemicals were tested in each assay. Potencies and bioactivity calls from the plate reader and imaging-based cell viability assays were comparable. The CP assay was more sensitive than either cell viability assay in that it detected a larger number of chemicals as bioactive, and phenotype altering concentrations (PACs) were lower than concentrations that decreased cell viability. An in vitro disposition (IVD) model that accounted for sorption of chemicals to plastic and cells over time was applied to predict freely dissolved PACs and compared with in vivo fish toxicity data. Adjustment of PACs using IVD modeling improved concordance of in vitro bioactivity and in vivo toxicity data. For the 65 chemicals where comparison of in vitro and in vivo values was possible, 59% of adjusted in vitro PACs were within one order of magnitude of in vivo toxicity lethal concentrations for 50% of test organisms. In vitro PACs were protective for 73% of chemicals. This combination of in vitro and in silico approaches has the potential to reduce or replace the use of fish for in vivo toxicity testing.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental reporting of fish transcriptomic responses in environmental toxicology and ecotoxicology.","authors":"Laura M Langan, Camille G Baettig, Alexander R Cole, Lea Lovin, Kendall Scarlett, Adam R Wronski, Megan E O'Brien, Yesmeena Shmaitelly, Bryan W Brooks","doi":"10.1093/etojnl/vgae077","DOIUrl":"https://doi.org/10.1093/etojnl/vgae077","url":null,"abstract":"<p><p>Due to its increasing affordability and efforts to understand transcriptional responses of organisms to biotic and abiotic stimuli, transcriptomics has become an important tool with significant impact on toxicological investigations and hazard and risk assessments, especially during development and application of new approach methodologies (NAMs). Data generated using transcriptomic methodologies have directly informed adverse outcome pathway frameworks, chemical and biological read across, and aided in the identification of points of departure. Using data reporting frameworks for transcriptomics data offers improved transparency and reproducibility of research and an opportunity to identify barriers to adoption of these NAMs, especially in environmental toxicology and ecotoxicology with aquatic models. Improved reporting also allows for reexamination of existing data, limiting needs for experiment replication and further reducing animal experimentation. Here, we use a standardized form of data reporting for omics-based studies, the Organisation for Economic Co-operation and Development omics reporting framework, which specifically reports on a list of parameters that should be included in transcriptomics studies used in a regulatory context. We focused specifically on fish studies using RNA- Sequencing (Seq)/microarray technologies within a toxicology context. Inconsistencies in reporting and methodologies among the experimental designs (toxicology vs. molecular characterization) were observed in addition to foundational differences in reporting of sample concentration or preparation or quality assessments, which can affect reproducibility and read across, confidence in results, and contribute substantially to understanding molecular mechanisms of toxicants and toxins. Our findings present an opportunity for improved research reporting. We also provide several recommendations as logical steps to reduce barriers to adoption of transcriptomics within environmental toxicology and ecotoxicology.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drinking water treatment residuals reduce toxicity to fish from metal-contaminated sediments.","authors":"Hajime G Kurita-Oyamada, Kevin J Kroll, Lang Zhou, Marianne Kozuch, Samuel M Wallace, Jean-François Gaillard, Jean-Claude J Bonzongo, Nancy D Denslow","doi":"10.1093/etojnl/vgae079","DOIUrl":"https://doi.org/10.1093/etojnl/vgae079","url":null,"abstract":"<p><p>Drinking water treatment residuals (DWTRs) produced as a result of the coagulation-flocculation process during water treatment are considered waste materials. Characterization of this material shows its ability to sequester metals and other anionic and cationic chemicals. Drinking water treatment residuals from two different drinking water treatment plants located in Wyoming and Oregon were evaluated for their ability to function as viable capping materials of metal-contaminated sediments. The contaminated sediments tested were either spiked with a mixture of metals, 1 mg/kg of Cu, Zn, Cd, and Pb, or coming from an intertidal sediment collected at a U.S. Naval Air Station. A Gust chamber experiment was used to determine metal fluxes from these sediments into the overlying water with applied hydrodynamic stress of 0.05 and 0.4 Pa in the absence and presence of DWTR as a capping material. The DWTR effectively reduced the amount of metal released to the overlying waters to a value below the National Recommended Aquatic Life Criteria for Cr, Cu, Pb, and Zn, but slightly above the value for Cd. The toxicity of these waters was tested with an in vivo 96 hr fathead minnow survival assay. In the absence of capping, all the fry died within 1 hr. Capping with DWTR from Wyoming effectively reduced contamination, and 95% of the fish survived. The DWTR from Oregon was less successful, but the survival of fish was equivalent to diluting the original contaminated waters by a factor of 100. Drinking water treatment residual effectively reduced metallothionein in fish, a biomarker of metal contamination, corroborating the survival experiments. These results suggest that DWTRs may be very effective for remediation of metal-contaminated sites.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of responses of cultured benthic foraminiferal communities to copper pollution through environmental RNA metabarcoding analysis.","authors":"Ling Qiao, Anran Zhao, Tao Yuan, Yuanming Guo, Ye Chen, Tiejun Li, Chengzhe Ren","doi":"10.1093/etojnl/vgae016","DOIUrl":"https://doi.org/10.1093/etojnl/vgae016","url":null,"abstract":"<p><p>Copper (Cu) is an essential trace element and can accumulate in sedimentary environments. However, beyond a certain concentration threshold, Cu can pose toxicity risks to biota. Benthic foraminifera are widely distributed and exhibit characteristics such as high biodiversity, short life cycle, good preservation potential, and high pollutant sensitivity, making them good bioindicators. In this study, we investigated the responses of benthic foraminiferal communities to various Cu concentrations through environmental RNA metabarcoding under controlled laboratory conditions. Some foraminiferal genera-Glandulina, Pyrgo, Quinqueloculina, Sorites, and Vellaria-exhibited sensitivity to high Cu concentrations. Other genera-Ammonia, Operculina, Reophax, Bolivina, Glabratella, Miliammina, and Parasorites-displayed tolerance to high Cu concentrations. Moreover, high Cu levels led to reduced living benthic foraminifera abundance and interspecific interactions, albeit without inducing test deformities. Our findings provide a novel and efficient approach for exploring the responses of benthic foraminiferal communities to heavy metal pollution.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":"44 1","pages":"159-168"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bumblebees prefer sulfoxaflor-contaminated food and show caste-specific differences in sulfoxaflor sensitivity.","authors":"Sarah E Orr, Jixiang Xu, Wanvimol C Juneau, Michael A D Goodisman","doi":"10.1093/etojnl/vgae007","DOIUrl":"https://doi.org/10.1093/etojnl/vgae007","url":null,"abstract":"<p><p>More than 30% of human food crop yield requires animal pollination. In addition, successful crop production depends on agrochemicals to control pests. However, agrochemicals can have negative consequences on beneficial insect pollinators, such as bees. We investigated the effects of an emerging class of pesticides, sulfoximines, on the common eastern bumblebee, Bombus impatiens. We performed a series of 96-hour toxicity tests on microcolonies of laboratory-reared B. impatiens. Our data showed that sulfoxaflor (SFX) is significantly less toxic to B. impatiens than historically used neonicotinoid pesticides, such as thiamethoxam. Further, for the first time, we found significant differences among castes in sensitivity to SFX; workers and drones were more sensitive than queens. These findings are notable because they reveal both caste and sex-specific differences in bumblebee sensitivity to pesticides. Interestingly, we found no evidence that bumblebees avoid SFX-contaminated sugar syrup. To the contrary, B. impatiens workers had an apparent preference for SFX-contaminated sugar syrup over sugar syrup alone. Overall, our investigation provides novel information on an important pesticide and may help inform regulatory decisions regarding pesticide use.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":"44 1","pages":"232-239"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diet and methyl mercury contamination of nestling red-winged blackbirds.","authors":"Thomas J Thalhuber, Matthew M Chumchal, Ray W Drenner, Weston H Nowlin, Dean A Williams, Benjamin D Barst, James H Kennedy, William A Mitchell, Misty Self, F Manton Willoughby, Will Zudock","doi":"10.1093/etojnl/vgae018","DOIUrl":"https://doi.org/10.1093/etojnl/vgae018","url":null,"abstract":"<p><p>Methyl mercury (MeHg) is a widespread environmental contaminant that can have adverse effects on the health of vertebrate wildlife. This study focused on diets and MeHg contamination of nestling red-winged blackbirds (Agelaius phoeniceus) from a wetland in north Texas, USA. In previous research at the study site, a risk assessment model suggested that if emergent aquatic insects (i.e., odonates) were the dominant prey item in nestling diets, the health of nestling red-winged blackbirds could be negatively affected. The purpose of this study was to follow up on an earlier risk assessment and determine whether nestling red-winged blackbirds were accumulating elevated concentrations of MeHg at our study site. We had four objectives: (1) estimate the proportion of emergent aquatic insects, spiders, and terrestrial insects in diets of nestling red-winged blackbirds using a stable isotope-based dietary mixing model; (2) assess the concentrations of MeHg in emergent aquatic insects, spiders, and terrestrial insects; (3) assess the concentrations of MeHg in blood of nestling red-winged blackbirds; and (4) determine whether nestling red-winged blackbirds had MeHg concentrations that were high enough to pose a health risk. We found that nestling red-winged blackbirds had a diet dominated by terrestrial insect prey with low concentrations of MeHg and that the nestlings had low concentrations of MeHg in their blood, well below hazardous levels. The results of the study suggest that caution must be used when interpreting risk assessment models for nestling red-winged blackbirds. Because their diets can consist of varying proportions of emergent aquatic insects, spiders, and terrestrial insects, risk assessments based on estimates of diet from the literature that suggest nestlings could be at risk from Hg contamination should be followed up with studies to assess diet and/or the actual level of contamination of nestlings.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":"44 1","pages":"59-67"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling time scale of integration in equilibrium passive sampling.","authors":"Oindrila Ghosh, Songjing Yan, Mandar Bokare, Upal Ghosh","doi":"10.1093/etojnl/vgae003","DOIUrl":"https://doi.org/10.1093/etojnl/vgae003","url":null,"abstract":"<p><p>Passive samplers (PSs) deployed in the field for several months provide a time-averaged measurement of the freely dissolved concentration of pollutants, which is important for assessing ecological exposure and estimating pollutant loads. A comprehensive theoretical modeling assessment of the sampling time scale of integration (TSI) of an equilibrium PS is required to correctly interpret the results. We address this knowledge gap by modeling exchange kinetics of polychlorinated biphenyl congeners in low-density polyethylene (PE) PS based on diffusive transport and first-order kinetics. We evaluate the sampling TSI by analyzing the response of the PS to simulated pulsed concentration increases in the water column that lasted for 1 day in a total sampling period of 90 days. More hydrophobic compounds experience slower transfer into the sampler and show a longer TSI compared with less hydrophobic compounds. Similarly, a thick sampler shows longer TSI than a thinner sampler. The sampling TSI for a typical 25.4 μm PE sheet ranged widely from 14-15 days for a dichlorobiphenyl to 43-45 days for a hexachlorobiphenyl. We show that strategic deployment of a thick and thin passive sampler can be used to narrow the range of TSIs for all congeners and used to simultaneously capture episodic events along with long-term averages.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":"44 1","pages":"68-76"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect-based analysis of endocrine effects in surface and ground water with focus on progestagenicity using Arxula yeast-based reporter gene assays.","authors":"Michelle Klein, Melissa Reibold, Petra Reinders, Fabian Itzel, Martin Jaehne, Linda Gehrmann, Martin Daniel Klaßen, Torsten Claus Schmidt, Jochen Türk","doi":"10.1093/etojnl/vgae045","DOIUrl":"10.1093/etojnl/vgae045","url":null,"abstract":"<p><p>The use of effect-based methods in water monitoring for identifying risks to aquatic organisms and human health is important for aiding regulatory decisions. In the past decades, the database on monitoring, especially in surface waters, has grown as this aquatic environment is openly exposed to various contamination sources. With regard to endocrine disruption, estrogenic and androgenic effects have been primarily investigated. Here, yeast-based bioassays emerged as potent tools, offering sensitivity to environmentally relevant concentrations and high robustness. The objectives of this study were to investigate further endocrine endpoints and extend the monitoring to ground waters. The inclusion of progestagenic effects is crucial due to their multifaceted roles in various functions of organisms. Hence, three different Arxula-yeast hormone screens (estrogen, androgen, and progesterone receptors) were applied, revealing simultaneous exposure to diverse endocrine effects in surface and ground water matrices. Although effect profiles in surface waters showed mainly activation of hormone receptors, in-ground water samples inhibitory effects clearly predominate. Although toxicological thresholds are not yet legally binding, they are essential for effective regulatory measures and risk management to ensure the good ecological status of aquatic ecosystems. The results were compared with effect-based trigger values for ecological as well as human risk assessment depending on the sample matrix, none of which were exceeded.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":"220-231"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting the toxicity of physically and chemically dispersed oil: a modelling case study with American lobster larvae (Homarus americanus).","authors":"Benjamin de Jourdan, Danielle Philibert, Joy McGrath","doi":"10.1093/etojnl/vgae012","DOIUrl":"https://doi.org/10.1093/etojnl/vgae012","url":null,"abstract":"<p><p>Determining the impact of an oil spill on aquatic ecosystems is a challenge. Because of the chemical complexity of crude oil, risk assessments rely on quantitative structure associated relationships to group chemical classes of compounds based on similar modes of toxicity. Quantitative structure associated relationships like the target lipid model can be used to determine species sensitivity by determining the critical target lipid body burden (CTLBB) and can be used to calculate the toxic units (TU) of a mixture. In this study we used the CTLBB generated from single polycyclic aromatic compound toxicity data and the analytical chemistry of whole oil to predicted and validate toxicity of both water-accommodated fraction (WAF) of crude oil and chemically dispersed WAF (CEWAF) to American lobster (Homarus americanus) larvae. A two-step procedure for modelling whole oil partitioning was utilized to compute the dissolved components in each of the WAF and CEWAF dilutions. Then, a species and life stage specific CTLBB derived for lobster larvae was applied in PetroTox to compute the TUs of exposure solution. The approach used in this study was able to effectively predict the effects observed in the exposures and can be integrated into oil spill fate and effects models to improve the oil spill assessment and response.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":"44 1","pages":"124-135"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}