Environmental Toxicology and Chemistry最新文献

{"title":"Integrating machine learning and nano-QSAR models to predict the oxidative stress potential caused by single and mixed carbon nanomaterials in algal cells.","authors":"Qi Qi, Zhuang Wang","doi":"10.1093/etojnl/vgae049","DOIUrl":"https://doi.org/10.1093/etojnl/vgae049","url":null,"abstract":"<p><p>In silico methods are increasingly important in predicting the ecotoxicity of engineered nanomaterials (ENMs), encompassing both individual and mixture toxicity predictions. It is widely recognized that ENMs trigger oxidative stress effects by generating intracellular reactive oxygen species (ROS), serving as a key mechanism in their cytotoxicity studies. However, existing in silico methods still face significant challenges in predicting the oxidative stress effects induced by ENMs. Herein, we utilized laboratory-derived toxicity data and machine learning methods to develop quantitative nanostructure-activity relationship (nano-QSAR) classification and regression models, aiming to predict the oxidative stress effects of five carbon nanomaterials (fullerene, graphene, graphene oxide, single-walled carbon nanotubes, and multi-walled carbon nanotubes) and their binary mixtures on Scenedesmus obliquus cells. We constructed five nano-QSAR classification models by combining zeta potential (ζP) with the C4.5 decision tree, support vector machine, artificial neural network, naive Bayes, and K-nearest neighbor algorithms. Moreover, we constructed three classification models by integrating the features including ζP, hydrodynamic diameter (DH), and specific surface area (SSA) with the logistic regression, random forest, and Adaboost algorithms. The Accuracy, Recall, Precision and harmonic mean of Precision and Recall (F1-score) values of these models were all higher than 0.600, indicating an excellent performance in distinguishing whether CNMs have the potential to generate ROS. In addition, using the ζP, DH, and SSA descriptors, we combined decision tree regression, random forest regression, gradient boosting, and the Adaboost algorithm, and successfully constructed four nano-QSAR regression models with applicable application domains (all training and testing data points lie within 95% confidence intervals), goodness-of-fit (Rtrain2 ≥ 0.850), and robustness (cross-validation R2 ≥ 0.650) as well as predictive power (Rtest2 ≥ 0.610). The method developed would establish a fundamental basis for more precise evaluations of ecological risks posed by these materials from a mechanistic standpoint.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parental exposure of the freshwater snail Planorbella pilsbryi to copper results in transgenerational effects in offspring.","authors":"Rebecca K Osborne, Patricia L Gillis, Ryan S Prosser","doi":"10.1093/etojnl/vgae050","DOIUrl":"https://doi.org/10.1093/etojnl/vgae050","url":null,"abstract":"<p><p>Although not assessed in standard ecotoxicological tests, exposure to a toxicant that does not result in an observable adverse effect in the parents may lead to transgenerational effects. These are adverse effects observed in unexposed offspring as a result of their parents' exposure to a toxicant. The goal of this study was to investigate whether transgenerational effects are observed in freshwater snails exposed to a toxicant. Using copper (Cu) as a reference toxicant, this study investigated whether the magnitude of exposure to the parents of the freshwater snail Planorbella pilsbryi resulted in a change in sensitivity of juvenile snails to a subsequent aqueous exposure of Cu. This study also investigated whether transgenerational effects observed in juvenile snails born to exposed parents would change if the parents laid eggs after being given time to recover from their exposure to Cu. Juvenile snails born to parents that were exposed to Cu without being given time to recover had no change in their sensitivity to Cu exposure or in some cases they became less sensitive. This study also observed that when parents were given time to recover from the Cu exposure, juvenile snails tended to be more sensitive to a subsequent Cu exposure, that is, decrease in median lethal concentrations for juveniles with an increase adult exposure. This study demonstrates that exposure of parent snails to a toxicant does not necessarily result in a consistent and significant change in sensitivity of the offspring to the same toxicant.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect-based analysis of endocrine effects in surface and ground water with focus on progestagenicity using Arxula yeast-based reporter gene assays.","authors":"Michelle Klein, Melissa Reibold, Petra Reinders, Fabian Itzel, Martin Jaehne, Linda Gehrmann, Martin Daniel Klaßen, Torsten Claus Schmidt, Jochen Türk","doi":"10.1093/etojnl/vgae045","DOIUrl":"https://doi.org/10.1093/etojnl/vgae045","url":null,"abstract":"<p><p>The use of effect-based methods in water monitoring for identifying risks to aquatic organisms and human health is important for aiding regulatory decisions. In the past decades, the database on monitoring, especially in surface waters, has grown as this aquatic environment is openly exposed to various contamination sources. With regard to endocrine disruption, estrogenic and androgenic effects have been primarily investigated. Here, yeast-based bioassays emerged as potent tools, offering sensitivity to environmentally relevant concentrations and high robustness. The objectives of this study were to investigate further endocrine endpoints and extend the monitoring to ground waters. The inclusion of progestagenic effects is crucial due to their multifaceted roles in various functions of organisms. Hence, three different Arxula-yeast hormone screens (estrogen, androgen, and progesterone receptors) were applied, revealing simultaneous exposure to diverse endocrine effects in surface and ground water matrices. Although effect profiles in surface waters showed mainly activation of hormone receptors, in-ground water samples inhibitory effects clearly predominate. Although toxicological thresholds are not yet legally binding, they are essential for effective regulatory measures and risk management to ensure the good ecological status of aquatic ecosystems. The results were compared with effect-based trigger values for ecological as well as human risk assessment depending on the sample matrix, none of which were exceeded.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyurethane microplastics and associated tris(chloropropyl)phosphate additives both affect development in larval fathead minnow Pimephales promelas.","authors":"Bonnie M Hamilton, Liisa M Jantunen, Chelsea M Rochman","doi":"10.1093/etojnl/vgae040","DOIUrl":"https://doi.org/10.1093/etojnl/vgae040","url":null,"abstract":"<p><p>Microplastics (< 5 mm) are a diverse class of contaminants ranging in morphology, polymer type, and chemical cocktail. Microplastic toxicity can be driven by one or a combination of these characteristics. Most studies, however, evaluate the physical effect of the most commercially available polymers. By disregarding other polymers with high consumption and/or production rates, and the chemical constituents of plastics, we fail to have a holistic understanding of the mechanisms of toxicity. Polyurethane is understudied in terms of effects testing yet considered one of the most hazardous polymers due to its chemical composition. Polyurethane is a high production polymer and is found in common consumer products ranging from packaging to spray foam insulation. To better understand the physico-chemical effects of polyurethane and a common additive in polyurethane products, we exposed larval fathead minnows for 28 days to polyurethane without chemical additives (i.e., plastic treatment), chemical leachate from polyurethane containing chemical additives (i.e., tris(chloropropyl)phosphate [TCPP]; i.e., chemical treatment) and polyurethane with chemical additives (i.e., plastic with chemical treatment) in a fully factorial experiment. We observed significant decreases in growth at 12 days posthatch (dph) in the plastic, chemical, and plastic with chemical treatments, suggesting a physical and chemical driver of toxicity. At 28 dph, we did not observe significant differences in growth, suggesting individuals can recover. We also observed concentrations of ΣTCPPs in fathead minnow exposed to the plastic with chemical treatment and the chemical only treatment, demonstrating TCPP uptake in exposed individuals. Combined, our data suggests the importance of both the physical and chemical components of microplastics when assessing effects, and thus emphasizing the need to evaluate the effects of microplastics in a multidimensional way.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative glucocorticoid receptor agonism: In silico, in vitro, and in vivo and identification of potential biomarkers for synthetic glucocorticoid exposure.","authors":"Alexander R Cole, Brett R Blackwell, Jenna E Cavallin, Jacob E Collins, Ashley R Kittelson, Yesmeena M Shmaitelly, Laura M Langan, Daniel L Villeneuve, Bryan W Brooks","doi":"10.1093/etojnl/vgae041","DOIUrl":"https://doi.org/10.1093/etojnl/vgae041","url":null,"abstract":"<p><p>The glucocorticoid receptor (GR) is present in almost every vertebrate cell and is utilized in many biological processes. Despite an abundance of mammalian data, the structural conservation of the receptor and cross-species susceptibility, particularly for aquatic species, has not been well defined. Efforts to reduce, refine, and/or replace animal testing have increased, driving the impetus to advance development of new approach methodologies (NAMs). Here we used in silico, in vitro, and in vivo methods to elucidate a greater understanding of receptor-mediated effects of synthetic glucocorticoid exposure in teleost fish. Evolutionary conservation of amino acid residues critical for transcriptional activation was confirmed in silico using sequence alignment to predict across species susceptibility. Subsequent in vitro assays using zebrafish and human GR provided evidence of physiological congruence of GR agonism. Finally, adult fathead minnows (Pimephales promelas) were exposed in vivo to the synthetic glucocorticoids, dexamethasone (0.04, 400, 4,000 µg/L) and beclomethasone dipropionate (130 µg/L), and GR agonism confirmed via digital polymerase chain reaction; in addition, EcoToxChip analyses identified potential mRNA biomarkers following glucocorticoid exposure. These findings support the use of NAMs to potentially reduce multispecies in vivo experimentation while providing empirical evidence that expands the taxonomic domain of applicability for the GR agonism molecular initiating event within the broader GR agonism adverse outcome pathway network.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the developmental, behavioral toxicity, and toxicological mechanism of the antidepressant drug venlafaxine and its active metabolites in zebrafish.","authors":"Qian Chen, Siying Gu, Yufen Lan, Jiaming Xu, Wenting Lin, Yingjun Qin, Yuan Ren","doi":"10.1093/etojnl/vgae055","DOIUrl":"https://doi.org/10.1093/etojnl/vgae055","url":null,"abstract":"<p><p>As a representative agent of bicyclic antidepressants, venlafaxine (VEN) has become widely used worldwide and is frequently detected in surface waters with concentrations ranging from ng/L to µg/L. To evaluate the toxicological effects of such medications on aquatic species, studies on environmentally relevant concentrations are essential. Zebrafish were used as a model organism to assess growth and development in larvae and examine tissue accumulation, oxidative stress, and DNA methylation in adults. The results showed adverse effects, including an 18.5% decrease in embryo hatching rate and an increase in mortality by 18.5%. There was also a reduction in body length (4.5%) and eye area (12.2%) in the larvae, along with abnormal developmental issues, such as pericardial edema, yolk sac edema, and spinal curvature. Venlafaxine and its metabolites induced oxidative stress, leading to observable toxic effects. In adult zebrafish, VEN and O-desmethylvenlafaxine (ODV) accumulated primarily in the liver, followed by the brain and intestines, and caused a reduction in DNA methyltransferase activity, leading to DNA hypomethylation. VEN had the most significant impact on DNA methyltransferase 1 and altered its conformation more than ODV. Overall, venlafaxine was found to be more toxic than its metabolites, providing a scientific basis for evaluating the toxic effects and ecological risks of antidepressant residues on aquatic organisms.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the Taskforce on Nature-related Financial Disclosures (TNFD) for chemical risk management with integrated ecotoxicology and ecology.","authors":"Kyoshiro Hiki, Kensuke Kito, Tatsuhiro Niino, Hiroshi Honda, Makoto Ishimota, Shiro Kawahara, Yuichi Iwasaki","doi":"10.1093/etojnl/vgae051","DOIUrl":"https://doi.org/10.1093/etojnl/vgae051","url":null,"abstract":"","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive effects of the insecticide acephate on a springtail and an enchytraeid in a subtropical soil.","authors":"Luís Carlos Iuñes Oliveira Filho, Douglas Alexandre, Letícia Scopel Camargo, Osmar Klauberg-Filho","doi":"10.1093/etojnl/vgae074","DOIUrl":"https://doi.org/10.1093/etojnl/vgae074","url":null,"abstract":"<p><p>The widespread use of acephate, a common insecticide, raises concerns about its potential impacts on nontarget soil organisms. This study investigated the chronic effects of acephate on the reproduction of two key soil fauna species, the springtail Folsomia candida and the enchytraeid Enchytraeus crypticus. We exposed these organisms to acephate in both natural Cambisol soil and tropical artificial soil (TAS) to assess potential impacts under different environmental conditions. Our results revealed significant reductions in reproduction for both species, with effects ranging from 38% to 49% (based on control and lowest observed effect concentration values). Furthermore, the observed effects were dependent on both the organism and the soil type. Springtails exhibited greater sensitivity in TAS than in Cambisol (0.09 and 15.0 mg a.i. kg-1 soil dry wt, respectively), whereas enchytraeids were more sensitive in Cambisol than in TAS (1 and 100 mg a.i. kg-1 soil dry wt, respectively). These findings highlight the importance of considering species-specific responses and soil properties when evaluating the ecological risks of pesticides on soil fauna communities.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial occurrence of emerging contaminants in rivers and wastewater. Analysis of environmental and human risks.","authors":"Carla Mariela Teglia, Fabiana Andrea Gutierrez, Selva Machado, Hérnan Ricardo Hadad, María Alejandra Maine, Héctor Casimiro Goicoechea","doi":"10.1093/etojnl/vgae075","DOIUrl":"https://doi.org/10.1093/etojnl/vgae075","url":null,"abstract":"<p><p>This study assesses the occurrence of emerging contaminants (ECs) from agricultural and livestock production activities along the Salado River (Santa Fe province, Argentina). Of the 23 ECs studied, 8 were detected and quantified in river and wastewater samples, including ciprofloxacin, enrofloxacin, chlorpyrifos-methyl, albendazole, fenbendazole, levamisole, diazepam, and thiamethoxam. In river samples, the highest concentrations corresponded to ciprofloxacin, chlorpyrifos-methyl, and enrofloxacin. In wastewater samples, albendazole, fenbendazole, ciprofloxacin, enrofloxacin, and thiamethoxam were found. The detection frequency ranged from 4.2% to 54.2% in river samples and from 11.1% to 22.2% in wastewater samples. The spatial distribution of contaminants showed different concentrations, with higher levels often found near urban and agricultural areas, suggesting anthropogenic sources. Ecological risk assessments for different organisms were conducted. Ciprofloxacin posed the highest risk, especially affecting bacteria, cyanobacteria, and algae. Diazepam also was found to pose significant risks to algae, crustaceans, and fishes. Chlorpyrifos-methyl was identified as highly hazardous to multiple living organisms, which is in agreement with the fish mortality that occurred at the sampling sites. The risk assessment in humans showed differences among children, adolescents, and adults, with infants (6 months to 1 year) being at a higher risk than adults. Children may be at a higher daily intake of contaminants than adults, raising concerns about the long-term effects of exposure. This work underscores the critical need for monitoring and regulating ECs in aquatic environments. Further studies are necessary to fully understand their impact and to develop effective strategies for mitigating their presence in water systems.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visualization of labeled micro- and nanoplastics in interaction with algae, using super-resolution stimulated emission depletion microscopy and fluorescence lifetime imaging.","authors":"Marie Sioen, Herlinde De Keersmaecker, Maaike Vercauteren, Colin Janssen, Jana Asselman","doi":"10.1093/etojnl/vgae068","DOIUrl":"https://doi.org/10.1093/etojnl/vgae068","url":null,"abstract":"<p><p>In contrast to microplastics, studying the interactions of nanoplastics (NPs) with primary producers such as marine microalgae remains challenging. This is attributed to the lack of adequate visualization methods that can distinguish NPs from autofluorescent biological material such as marine algae. The aim of this study was to develop a method for labeling and visualizing nonfluorescent micro- and nanoplastics (MNPs) of various polymer types, shapes, and sizes, in interaction with marine primary producers, which are autofluorescent. A labeling technique for plastics was refined, using a swell incorporation method with the commercial dye \"IDye.\" Comprehensive quality control measures, including toxicity, leaching, and dye longevity tests, were applied to ensure the robustness of the method. Although stimulated emission depletion (STED) microscopy successfully enabled the visualization of the diverse labeled NPs smaller than 200 nm, it could not distinguish NPs from autofluorescent organic material such as marine microalgae, due to overlapping excitation and emission spectra with the photosynthetically active molecule chlorophyll-a. This study is the first to advance the field by coupling STED with fluorescence lifetime imaging microscopy (FLIM). The FLIM technique, based on the differing lifetimes of fluorescent signals, allowed us to overcome the challenge of overlapping spectra. Our work not only refines and expands existing plastic labeling protocols to accommodate a wide range of polymer types, but also introduces a more precise method for studying interactions between MNPs and autofluorescent organisms. This combined STED-FLIM approach provides a reproducible and reliable framework for examining MNP impacts in complex, ecologically relevant environments, particularly highlighting its potential for investigating MNP-microalgae interactions.</p>","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}