Egyptian Pharmaceutical Journal最新文献_第3页

Study of prevalence of parasitic intestinal infections in a tertiary care center in Central India serving a rural population: a retrospective analysis 印度中部一家为农村人口服务的三级护理中心寄生虫肠道感染流行率的研究：回顾性分析

Egyptian Pharmaceutical Journal Pub Date : 2023-04-01 DOI: 10.4103/epj.epj_151_22

Priyanka Chaubey, S. Sande, Ashwini Mankar

{"title":"Study of prevalence of parasitic intestinal infections in a tertiary care center in Central India serving a rural population: a retrospective analysis","authors":"Priyanka Chaubey, S. Sande, Ashwini Mankar","doi":"10.4103/epj.epj_151_22","DOIUrl":"https://doi.org/10.4103/epj.epj_151_22","url":null,"abstract":"Backgrouud Intestinal parasitic infections is a globally prevalent condition with variable morbidities like bleeding, iron-deficiency anemia, megaloblastic anemia, intestinal obstruction, fat malabsorption, hepatosplenomegaly, and mortality. It is also responsible for retardation in growth and poor mental development in growing children, which is the worst attacked age group. Its prevalence varies from geographical regions like tropical and subtropical and also varies from populations like in urban, suburban, and rural. Periodic monitoring of prevalence to set control programs for that locality is needed. Aim To study the prevalence of various parasitic intestinal infections in a rural population in Central India. Materials and methods This descriptive and retrospective study was done in a 350-bed tertiary care hospital located in Central India serving a rural population. We have extensively studied all stool samples collected and reported between August 2019 and May 2022. Results A total of 1120 stool samples were reported during our study period, of which 518 were of females and 602 were of males. Of these 1120 stool samples, 91 samples showed the presence of parasite. The overall prevalence of intestinal parasitic infection in our study population is 8.13%, whereas the prevalence of the same in males and females is 8.63 and 7.53%, respectively. Conclusion We have found no association or effect of sex on intestinal parasitic infections in our study population. The prevalence of the same was lower in our rural population compared with various other studies, indicating good hygiene level in the society. The prevalence was highest in 21–40-year age group. Age group had a significant association with intestinal parasitic infections in our study population.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"22 1","pages":"223 - 228"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48920221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The pharmacological impact of Astragalus membranaceus against coccidial and bacterial infection in vitro 黄芪体外抗球虫和细菌感染的药理作用

Egyptian Pharmaceutical Journal Pub Date : 2023-04-01 DOI: 10.4103/epj.epj_3_23

Reem M. Ramadan, F. Youssef, E. Fouad, A. Orabi, M. Khalifa

{"title":"The pharmacological impact of Astragalus membranaceus against coccidial and bacterial infection in vitro","authors":"Reem M. Ramadan, F. Youssef, E. Fouad, A. Orabi, M. Khalifa","doi":"10.4103/epj.epj_3_23","DOIUrl":"https://doi.org/10.4103/epj.epj_3_23","url":null,"abstract":"Background Astragalus polysaccharides (APS) are a novel macromolecule extracted from the herbal plant Astragali radix with potential biological activity such as antioxidant, anti-inflammatory, antidiabetic, anticancer, and immunomodulatory properties. Objectives The present research emphasizes on some of the biological characteristics of this product including its phytochemical screening, its effective LD50, its antioxidant, anti-inflammatory, anticoccidial, and antimicrobial activities in vitro. Materials and methods Phytochemical screening of the tested extract proved that it contained alkaloids, flavonoids, and glycoside components. Testing its efficacy as bactericidal versus Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae, Pasteurella multocida and Staphylococcus aureus its value as a coccidiocidal drug against five chicken Eimeria species oocysts and its effect on the level of DNA genotoxic damage using comet assay proved high significant efficacy (P≤0.05) in the form of marked inhibition zone of bacteria, considerable sporulation inhibition percentage in oocysts as well as high genotoxic damages in the DNA. Result and conclusion The study proved the presence of a direct relationship between the increase in APS concentrations and exposure time and the rate of sporulation inhibition and DNA damage in oocysts subjected to various doses of APS. This DNA damage was determined by marked variations in tail’s length (µm), the percentage of DNA in the tail segment, and tail’s moment were used to demonstrate this relationship (µm). In conclusion, APS proved to be a potential herbal to have anticoccidial and antibacterial attributes in controlling both infections in chickens.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"22 1","pages":"324 - 335"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41418917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Involvement of host iron-withholding strategy on Streptococcus pyogenes strain KSU-1 growth and pathogenicity 宿主铁抑制策略对化脓性链球菌KSU-1株生长和致病性的影响

Egyptian Pharmaceutical Journal Pub Date : 2023-04-01 DOI: 10.4103/epj.epj_165_22

M. Alkhulaifi, A. Al-Salamah, Eman Elgammal, A. Ibrahim

{"title":"Involvement of host iron-withholding strategy on Streptococcus pyogenes strain KSU-1 growth and pathogenicity","authors":"M. Alkhulaifi, A. Al-Salamah, Eman Elgammal, A. Ibrahim","doi":"10.4103/epj.epj_165_22","DOIUrl":"https://doi.org/10.4103/epj.epj_165_22","url":null,"abstract":"Background and objectives Streptococcus pyogenes is a highly adaptable human pathogen that can cause a wide spectrum of infections ranging from mild to a life-threating systemic infection. This study discussed the effectiveness of iron-depriving strategy on growth, survival, and virulence of S. pyogenes. Materials and methods Some comparisons between different iron-saturated and iron-depleted forms of the main human iron reservoirs (hemoglobin, hemin, transferrin, lactoferrin, and human milk) were tested for their effect on growth and pathogenicity of S. pyogenes. Results and conclusion Although the iron-saturated forms enhanced the growth and survival, the iron-free forms had a bacteriostatic/bactericidal activity against the microbe, and these results were emphasized by the in vivo study. Finally, the bacterial surface proteins as virulence factors were secreted upon iron depletion as indicated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. This study proved that iron-depletion environment increased the resistance and virulence factors of S. pyogenes. Moreover, these results can give an insight about the interaction between the host and pathogen, which can lead to designing of new antimicrobial agents or vaccine that may target these pathways.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"22 1","pages":"229 - 236"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47113353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategies for cancer therapy: targeting tumor microenvironment and nanotechnology 肿瘤治疗策略:靶向肿瘤微环境和纳米技术

Egyptian Pharmaceutical Journal Pub Date : 2023-04-01 DOI: 10.4103/epj.epj_188_22

A. Abdel-Halim

引用次数: 0

Prospective of healthcare professionals regarding pharmacogenomics 医疗保健专业人员对药物基因组学的展望

Egyptian Pharmaceutical Journal Pub Date : 2023-04-01 DOI: 10.4103/epj.epj_175_22

Anuradha T Deshkar, Sonali B. Rode

{"title":"Prospective of healthcare professionals regarding pharmacogenomics","authors":"Anuradha T Deshkar, Sonali B. Rode","doi":"10.4103/epj.epj_175_22","DOIUrl":"https://doi.org/10.4103/epj.epj_175_22","url":null,"abstract":"Background and objective Pharmacogenomics is an emerging branch and its application in clinical practice will improve healthcare delivery in India. For the promotion and adaptation of pharmacogenomics, we need baseline data from healthcare professionals (HCPs). So, this study was planned to know the prospective of HCPs in India regarding pharmacogenomics. Patients and methods The printed copies of the pretested and validated, single-response or multiple-response self-administered questionnaire, were distributed to the HCPs. There was a total of 19 questions to access knowledge, attitude, and practices regarding pharmacogenomics. Data obtained were entered in Microsoft Excel 2013 spreadsheet for statistical analysis and percentages were calculated for concerned responses. Results Out of 134 responses, 126 responses were found to be eligible for analysis. Seventy-eight (61.9%) participants scored more than or equal to 5 to show above-average knowledge. Ninety (71.4%) participants showed a favorable attitude with a score of more than or equal to 3, while 91 (72.2%) participants scored more than or equal to 3 to show favorable practices regarding pharmacogenomics. Hundred and seven (84.9%) participants believed that pharmacogenomics will lead to more effective pharmacotherapy. Conclusion Participants showed above-average knowledge; favorable attitude, and practice behavior regarding pharmacogenomics. The survey indicates that conducting Information, Education, and Communication activities may help to increase awareness and knowledge, and to promote the adoption and utilization of pharmacogenomics in clinical practice, which will lead to better pharmacological treatment of disease with maximum efficacy and minimum side effects.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"22 1","pages":"259 - 264"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43195617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Staphylococcus aureus virulence genes and methicillin-resistant gene detection and antimicrobial resistance profiles isolated from different infection sites 不同感染部位分离的金黄色葡萄球菌毒力基因和耐甲氧西林基因检测及耐药谱

Egyptian Pharmaceutical Journal Pub Date : 2023-04-01 DOI: 10.4103/epj.epj_187_22

Rafal Ismael, A. Alhameedawi, Rajaa Abbas, Sarah Alsallameh, Halah Amer, Müge Firat, M. Karkhane

{"title":"Staphylococcus aureus virulence genes and methicillin-resistant gene detection and antimicrobial resistance profiles isolated from different infection sites","authors":"Rafal Ismael, A. Alhameedawi, Rajaa Abbas, Sarah Alsallameh, Halah Amer, Müge Firat, M. Karkhane","doi":"10.4103/epj.epj_187_22","DOIUrl":"https://doi.org/10.4103/epj.epj_187_22","url":null,"abstract":"Background One of the most significant pathogenic bacteria is Staphylococcus aureus, and both adults and children are susceptible to this bacterium from the front of the nose. In the United States, the Centers for Disease Control and Prevention estimate that 80,461 invasive methicillin-resistant S. aureus (MRSA) infections and 11 285 related deaths occurred in 2011. In the UK, around 190 people passed away from MRSA disease in 2021. Australia, Hong Kong, Singapore, Japan, and Greece also have MRSA infections, along with the whole world. MRSA caused less than 2% of bacterial diseases in the United States in 1974, while the percentage rate jumped to 64% in 2004 only 10 years to increase the infection rate by 300%. Objective This study aimed to detect medication susceptibility patterns, staphylococcal enterotoxins A to C, toxic shock syndrome toxin-1, and methicillin-resistant genes. Materials and methods Ninety-eight S. aureus strains were isolated from different infection sites from Salah Al-Din Teaching Hospital. There have only been a few studies conducted on the epidemiology and virulence genes of S. aureus in Salah Al-Din city, Iraq. Results and conclusion The rates of drug resistance among S. aureus strains to routinely used antibiotics were found to be extremely high. In this study, the expression of toxic shock syndrome toxin, sec B, and sec C genes in S. aureus strains was not detected, unlike in previous studies. While all the strains were sec A gene positive, another gene found in bacterial cells that enables them to be resistant to antibiotics like methicillin and other vancomycin drugs is mecA.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"22 1","pages":"265 - 271"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44477871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis and FPLC purification of antibacterial peptide from the biotherapeutic agent Enterococcus faecium 粪肠球菌生物治疗剂抗菌肽的生物合成及FPLC纯化

Egyptian Pharmaceutical Journal Pub Date : 2023-04-01 DOI: 10.4103/epj.epj_143_22

Eslam Abd-elwahed, A. El-Waseif, D. Maany

{"title":"Biosynthesis and FPLC purification of antibacterial peptide from the biotherapeutic agent Enterococcus faecium","authors":"Eslam Abd-elwahed, A. El-Waseif, D. Maany","doi":"10.4103/epj.epj_143_22","DOIUrl":"https://doi.org/10.4103/epj.epj_143_22","url":null,"abstract":"Background Probiotics are microorganisms that play an essential role in microbial intestinal balance and in health care. Objective To isolate a probiotic that can be used to produce antimicrobial peptides potentially used as inhibitors against pathogenic bacteria. Materials and methods The research protocol was carried out through isolation of samples from different dairy product and screening for the most potent probiotic exhibiting antimicrobial activity against Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 8739 according to the guidelines of Clinical and Laboratory Standards Institute using the disc diffusion method. The molecular identification of this probiotic strain was done by 16S ribosomal DNA sequencing, and the phylogenetic tree was obtained. The purification process and characterization of the antibacterial peptide were done by (NH4)2SO4 and performing fast protein liquid chromatography. Results and discussion Bacterial probiotic strains obtained from different samples were screened for the best antimicrobial activity, where isolate number 9 from 18 isolates showed the highest antibacterial activity against S. aureus and E. coli. Therefore, it was chosen for molecular identification. The molecular identification process revealed that isolate number 9 was Enterococcus faecium. Results of antibiotics sensitivity indicated that S. aureus is more sensitive to antibiotics than E. coli. The fast protein liquid chromatography purification and characterization process of the peptide produced from the probiotic E. faecium showed that the active fraction was precipitated at 60% saturation of (NH4)2SO4. Moreover, single absorbance peaks confirmed the presence of the peptide ‘enterocin.’","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"22 1","pages":"202 - 208"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70732822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Angiopoietin-like protein 5: a potential culprit of cardio-metabolic risk in Egyptian obese children 血管生成素样蛋白5：埃及肥胖儿童心脏代谢风险的潜在罪魁祸首

Egyptian Pharmaceutical Journal Pub Date : 2023-04-01 DOI: 10.4103/epj.epj_168_22

Mai ElShiekh, Ghada M. El-Kassas, Maged A. El Wakeel, Dina Zaki, Hanan Atta, Dina Mostafa, S. El-Zayat, G. Elsawy

{"title":"Angiopoietin-like protein 5: a potential culprit of cardio-metabolic risk in Egyptian obese children","authors":"Mai ElShiekh, Ghada M. El-Kassas, Maged A. El Wakeel, Dina Zaki, Hanan Atta, Dina Mostafa, S. El-Zayat, G. Elsawy","doi":"10.4103/epj.epj_168_22","DOIUrl":"https://doi.org/10.4103/epj.epj_168_22","url":null,"abstract":"Background Obesity has recently been identified as a risk factor for coronavirus disease-19. There is a significant correlation between obesity rates with metabolic syndrome and coronavirus disease-19 in children worldwide. Owing to the high expression in adipose tissue, angiopoietin-like protein 5 (ANGPTL5) might be assumed to have a broad range of physiological processes in lipid, glucose homeostasis, and inflammatory responses. Objective To examine the association between childhood obesity and plasma ANGPTL5 levels in an attempt to demonstrate the probable relationships of plasma levels of ANGPTL5 with unacylated ghrelin and obestatin and its relevance to metabolic parameters in obese versus normal-weight children and adolescents. Patients and methods A total of 90 children between 5 and 15 years of age were randomly enrolled in this cross-sectional case–control study. They were classified into obese patients (n=45) and nonobese controls (n=45). BMI for age was calculated following WHO guidelines. Serum levels of ANGPTL5, obestatin, and unacylated ghrelin were measured and correlated with the anthropometric measurements and biochemical markers of metabolic syndrome. Results A highly significant positive correlation was observed between the serum level of ANGPTL5 and the weight z score. Moreover, a significant positive correlation between ANGPTL5 and the investigated diabetic parameters (glucose, insulin, and homeostasis model assessment for insulin resistance) and high sensitivity C-reactive protein was detected. The increased weight in children was associated with a higher level of ANGPTL5 in parallel with the observed elevated insulin resistance. Conclusion ANGPTL5 might be considered a promising sensitive assessment biomarker to be applied for early diagnosis and prognosis of obese children with a high risk of cardio-metabolic diseases.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"22 1","pages":"251 - 258"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43272126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statistical optimization for polyhydroxybutyrate production by locally isolated Bacillus safensis using sugarcane molasses under nutritional stressed conditions 营养胁迫条件下本地分离的安全芽孢杆菌利用甘蔗糖蜜生产聚羟基丁酸酯的统计优化

Egyptian Pharmaceutical Journal Pub Date : 2023-04-01 DOI: 10.4103/epj.epj_51_22

Sanaa K. Gomaa, H. El-Refai, R. Allam, M. Shafei, H. Ahmed, Rania A. Zaki

{"title":"Statistical optimization for polyhydroxybutyrate production by locally isolated Bacillus safensis using sugarcane molasses under nutritional stressed conditions","authors":"Sanaa K. Gomaa, H. El-Refai, R. Allam, M. Shafei, H. Ahmed, Rania A. Zaki","doi":"10.4103/epj.epj_51_22","DOIUrl":"https://doi.org/10.4103/epj.epj_51_22","url":null,"abstract":"Background and objective Biodegradable polymers, such as polyhydroxyalkanoate (PHAs), have recently been recognized as polyesters owing to their properties as biodegradable thermoplastics. Materials and methods The main objective of this study was to isolate an efficient polyhydroxybutyrate (PHB) producer from soil collected from different rhizospheric areas in Egypt. The potent strain was identified using 16 s rRNA sequence analysis. Plackett–Burman and central composite sequential designs were used to investigate cultural variables influencing PHB production (central composite sequential design). Results and conclusion Of a total of nine different isolates, three were found to be PHB positive based on the color using Nile Red stain. The potent strain was identified as Bacillus safensis (NR_113945.1). Using one factor at a time experiments, sugar molasses and ammonium sulfate, respectively, were the best carbon and nitrogen sources, whereas the best inoculum was 10 ml/100 ml of fermentation medium. Cultural variables influencing PHB production were studied using Plackett–Burman and central composite sequential designs. Accordingly, the most influential factors on PHB production were sugarcane concentration, inoculum size, and KH2PO4. Under the optimized conditions, a PHB content (93% cell dry weight) of 1.17 folds was attained. Furthermore, the Fourier transform infrared spectroscopy and 1H-NMR results confirmed the produced polymer as PHB. These results give insight into the use of locally isolated bacteria (B. safensis (NR_113945.1) utilizing inexpensive substrate such as sugarcane molasses for PHB production.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"22 1","pages":"192 - 201"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48982486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemicals and biological activities of caraway (Carumcarvi L.) essential oil 香菜精油的植物化学成分及生物活性研究

Egyptian Pharmaceutical Journal Pub Date : 2023-04-01 DOI: 10.4103/epj.epj_154_22

M. El-Rady, N. Rasmy, N. Yasin, Hanea Fahmy, A. Amer

{"title":"Phytochemicals and biological activities of caraway (Carumcarvi L.) essential oil","authors":"M. El-Rady, N. Rasmy, N. Yasin, Hanea Fahmy, A. Amer","doi":"10.4103/epj.epj_154_22","DOIUrl":"https://doi.org/10.4103/epj.epj_154_22","url":null,"abstract":"Background and aim Caraway is a famous medicinal plant in various pharmaceutical, food, and cosmetic industries. This study aimed to investigate the chemical composition, antioxidant, antimicrobial, and anticancer activities of this plant’s essential oil (EO). Materials and methods Caraway EO was obtained from dried caraway seeds using the hydrodistillation process. The composition of caraway EO was inspected by gas chromatography-mass spectrometry (GC–MS) analyses. The antioxidant activity of caraway EO was determined by three different in vitro antioxidant assays: 2,2-diphenylpicrylhydrazyl (DPPH•), 2,2’-azino-bis3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+) scavenging activity and reducing power. The agar well diffusion method was used to assess the antimicrobial action. The cytotoxic activity was evaluated using the MTT (3-(4, 5-dimethyl thiazol-2yl)-2, 5-diphenyl tetrazolium bromide) assay, and the data were expressed as the half-maximal inhibitory concentration (IC50). Results and conclusion Carvone was the major compound of caraway EO, followed by limonene. Estimation of the antioxidant activity using DPPH• scavenging activity, ABTS•+ scavenging activity, and reducing power assays revealed effective efficacy [IC50=32.46±0.75, 2.44±0.44, and 17.65±0.70 µg/ml, respectively, compared with 11.55±0.53, 1.50±0.29, and 23.19±0.78 µg/ml for standard control (butylated hydroxyanisole), respectively]. Strong anticancer activity was detected against all types of cancer cells, especially the colon cell line (HCT-116) and liver cell line (HepG-2). These results suggest that caraway EO can be used as a preservative food agent in food industries as well as in the field of pharmacy, as it presents promising anticancer properties.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"22 1","pages":"285 - 293"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47366683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0