黄芪体外抗球虫和细菌感染的药理作用

IF 0.7 Q4 PHARMACOLOGY & PHARMACY
Reem M. Ramadan, F. Youssef, E. Fouad, A. Orabi, M. Khalifa
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引用次数: 2

摘要

背景黄芪多糖(APS)是从黄芪中提取的一种新的大分子,具有抗氧化、抗炎、抗糖尿病、抗癌和免疫调节等潜在的生物活性。目的研究该产品的一些生物学特性,包括其植物化学筛选、有效LD50、抗氧化、抗炎、抗球虫和体外抗菌活性。材料与方法经植物化学筛选,该提取物含有生物碱、黄酮类和糖苷类成分。通过彗星试验测试其对大肠杆菌、鼠伤寒沙门氏菌、肺炎克雷伯菌、多杀性巴氏杆菌和金黄色葡萄球菌的杀菌效果,其作为对五种鸡艾美耳球虫卵囊的杀球虫药物的价值及其对DNA基因毒性损伤水平的影响,证明其具有显著的细菌抑制区形式的高效性(P≤0.05),卵囊中相当大的孢子形成抑制百分比以及DNA中的高遗传毒性损伤。结果和结论本研究证明,APS浓度的增加和暴露时间与不同剂量APS对卵囊孢子形成的抑制率和DNA损伤率之间存在直接关系。这种DNA损伤是通过尾巴长度(µm)、尾巴片段中DNA的百分比和尾巴力矩的显著变化来确定的。总之,APS被证明是一种具有抗球虫和抗菌特性的潜在草药,可以控制鸡的两种感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The pharmacological impact of Astragalus membranaceus against coccidial and bacterial infection in vitro
Background Astragalus polysaccharides (APS) are a novel macromolecule extracted from the herbal plant Astragali radix with potential biological activity such as antioxidant, anti-inflammatory, antidiabetic, anticancer, and immunomodulatory properties. Objectives The present research emphasizes on some of the biological characteristics of this product including its phytochemical screening, its effective LD50, its antioxidant, anti-inflammatory, anticoccidial, and antimicrobial activities in vitro. Materials and methods Phytochemical screening of the tested extract proved that it contained alkaloids, flavonoids, and glycoside components. Testing its efficacy as bactericidal versus Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae, Pasteurella multocida and Staphylococcus aureus its value as a coccidiocidal drug against five chicken Eimeria species oocysts and its effect on the level of DNA genotoxic damage using comet assay proved high significant efficacy (P≤0.05) in the form of marked inhibition zone of bacteria, considerable sporulation inhibition percentage in oocysts as well as high genotoxic damages in the DNA. Result and conclusion The study proved the presence of a direct relationship between the increase in APS concentrations and exposure time and the rate of sporulation inhibition and DNA damage in oocysts subjected to various doses of APS. This DNA damage was determined by marked variations in tail’s length (µm), the percentage of DNA in the tail segment, and tail’s moment were used to demonstrate this relationship (µm). In conclusion, APS proved to be a potential herbal to have anticoccidial and antibacterial attributes in controlling both infections in chickens.
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来源期刊
Egyptian Pharmaceutical Journal
Egyptian Pharmaceutical Journal PHARMACOLOGY & PHARMACY-
CiteScore
1.10
自引率
0.00%
发文量
37
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