Egyptian Pharmaceutical Journal最新文献

{"title":"Effect of potassium fertilization on growth, yield, and some active ingredients of Prunella plant","authors":"Shaimaa I. M. Elsayed, R. Fouad, H. Fouad, S. Hendawy, E. Omer","doi":"10.4103/epj.epj_9_23","DOIUrl":"https://doi.org/10.4103/epj.epj_9_23","url":null,"abstract":"Background Prunella vulgaris L. is a perennial herb belonging to the Lamiaceae family, it is used as antibacterial, antipyretic, antiseptic, antispasmodic, astringent, carminative, diuretic, febrifuge, hypotensive, stomachic, septic, tonic, vermifuge, and vulnerary properties are all seen to be alternatives to whole plants. Potassium is an important macronutrient for plants because of its ability to affect meristem development, water status, photosynthesis, long-distance assimilation of nutrients, enhancement of some enzyme functions, and regulate ionic equilibrium. Objective To investigate the effects of various potassium fertilizer applications and the quantity of additions on the growth, production, and some chemical constituents of the P. vulgaris plant. Materials and methods Prunella plants were cultivated at SEKEM Company Farm, Belbes, Sharkia, using a drip irrigation system during the two successive seasons of 2018/2019 and 2019/2020 to study the impact of application of different potassium doses (20, 40, and 60 U) and number of added portions (each dose added once or divided into two or three portions) on plant diameter, fresh and dry weight of herb (g/plant and ton/ha), and fresh and dry weight of flowering clusters at both seasons. Photosynthetic pigments in leaves (mg/g fresh leaves), total phenols (mg/g dry herb and flowering clusters), and antioxidant activity (%) in herb and flowering clusters and nitrogen, phosphorus, and potassium concentrations (%) were determined. Results and conclusion Increasing units of potassium fertilization from 20 to 60 U gave the highest plant diameter, fresh and dry weight of herb, and fresh and dry weight of flowering clusters. It also led to an intensification of total phenolics, antioxidant activity %, chlorophyll content, and N, P, and K percentages of all Prunella herbs, whether it was added at two or three portions.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"22 1","pages":"440 - 448"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49611699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of melatonin on oxidative stress of differentiated Dopaminergic cells","authors":"H. Ibrahim, Abdelaziz M. Hussein, Mahmoud Gabr, Abdel A.F., Rasha El-Saeed","doi":"10.4103/epj.epj_22_23","DOIUrl":"https://doi.org/10.4103/epj.epj_22_23","url":null,"abstract":"Background Although stem cells therapies provide a great deal in the treatment of several disease, they lack their normal functions after transplantation due to inflammation and oxidative stress. Melatonin has a powerful antioxidant ability and can enhance the effect of stem cells. Objectives This work aimed to investigate the melatonin’s effect on oxidative stress of differentiated adipose-mesenchymal stem cells (AD-MSCs) to dopaminergic (DAergic) cells. Material and methods The AD-MSCs cells were characterized after passage 3 by flow cytometry method and divided into four groups: (a) control group that was nontreated AD-MSCs, (b) MSCs+M group that was AD-MSCs cultured with 1 μM melatonin in expansion media for 12 days, (c) DN group that was MSCs treated with neurobasal A media for 12 days, (d) DN+M group which was MSCs cultured with 1 μM melatonin and neurobasal A media for 12 days. After 12 days, the catalase (CAT) activity and malondialdehyde (MDA) level were measured by using ELISA. Also, the gene expression level of MAP-2 was detected. Results and conclusion The current study proved that the isolated cells were MSCs due to high expression percentages for CD73 and CD90 and low expression percentages for CD34 and CD45. The DN+M group showed the highest expression of MAP-2 gene when compared to the other different groups (P ≤ 0.05). Moreover, there was a significant increase in CAT concentration in groups treated with melatonin than other group (P ≤ 0.05), while, there was no change in MDA level between all groups. It was concluded that melatonin has an effective antioxidative role throughout the differentiation process of AD-MSCs into DAergic neural cells","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"9 1","pages":"481 - 489"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139364454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New molecule of nonribosomal peptide synthesis mechanism from Lactiplantibacillus plantarum LMG100 probiotic bacteria","authors":"Amr Elmasry, Walaa Hussein, Ali Abdelmoteleb","doi":"10.4103/epj.epj_46_23","DOIUrl":"https://doi.org/10.4103/epj.epj_46_23","url":null,"abstract":"Background The huge increasing on gastrointestinal illness by spreading of resistance pathogens requires to develop alternative antimicrobial agents. Nonribosomal peptides are considered one of these alternatives which produced by wild spectrum of bacteria. Objective Detection of nonribosomal peptide synthesis from Lactiplantibacillus plantarum LMG100 probiotic strain isolated from traditional lactic fermenting foods as alternative antimicrobial agent is important to human health and immune system. Materials and methods The identification of isolated strains using 16S rDNA technique was performed and followed by bioinformatics analysis tools; AntiSmash, PKS-NRPS analysis website, LSI based A-domain function predictor, NRPS predictor2, clustering using PhyML 3.0 to detect adenylation domain substrate specificity of NRP synthetases genes cluster of Lactiplantibacillus plantarum LMG100. To prove the presence of the NRP synthetases genes cluster, degenerate primers protocol and three sets of primers covered the five gene cluster were designed based on the original reference strain L. plantarum WCFS1. Antibacterial activity of the isolated strain was detected against bacterial strains from coliform group of the enteric genera of Escherichia, Salmonella and Shigella which formed the ordinary gastrointestinal tract infection. Results and conclusion The isolated L. plantarum LMG100 strain showed 99.96% of identity to 16S rDNA partial gene sequence of Lactiplantibacillus plantarum WCFS1 strain and bioinformatics analysis tools revealed the presence of NRPS gene cluster of five genes; two biosynthetic genes npsA and npsB from the five genes encoded for polypeptide of six amino acids, but six different predictors programs couldn’t assign the specificity of all adenylation domains except A4 serine and A5 glycine. The use of degenerate primers confirmed the presence of the NRPS in the isolated strain L. plantarum LMG100 compared to the standard strain WCFS1. Three sets of primers covering the five gene cluster were designed based on the original reference strain L. plantarum WCFS1 complete genome sequence confirmed the same organization of the putative gene cluster. In general, the approach of degenerated primers proved the presence of polypeptide NRPs gene presence in lactobacilli isolates. The new polypeptide NRP produced from the strain LMG100 showed maximum inhibition zones against G-ve short-rods bacterial strains from the coliform group of the enteric genera of Escherichia, Salmonella and Shigella which formed the ordinary gastrointestinal tract infection, and the minimal inhibitory concentrations (MIC) for G-ve bacterial strains was approximately of 125 mg.ml−1. The obtained results revealed that the selected probiotic Lactiplantibacillus strain is suitable candidate for use as bio-preservative starter or probiotic for human consumption in food and pharmaceutical industries.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"4 1","pages":"380 - 390"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139364994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of different carbon sources on callus formation, pigment accumulation, and antiviral activity in cell cultures of Euphorbia milii","authors":"N. Arafa, Ahmed Amer, Nancy Girgis, Reem El-Shenawy, Naiera M Helmy","doi":"10.4103/epj.epj_19_23","DOIUrl":"https://doi.org/10.4103/epj.epj_19_23","url":null,"abstract":"Background and aim Humans have long been relying on plants for food, shelter, and most importantly for medicines. In vitro culture techniques, which ensure rapid, consistent, and uniform mass production, are indispensable for manufacturing important plant-derived metabolites. However, insufficient information is available regarding the tissue culture of Euphorbia milii and its important bioactive compounds. This study mainly aims to explore the impact of different carbon sources on callus formation, pigment accumulation, and antiviral activity of E. milii. Materials and methods Using inflorescence tissues as explants, we investigated the effects of different types and concentrations of plant growth regulators (2,4-dichlorophenoxyacetic acid [2,4-D], alone or combined with benzyl aminopurine) on callus induction frequency. Moreover, we studied the effects of different concentrations of carbon sources (glucose, fructose, and mannose) on callus growth, pigment (chlorophylls, carotenoids, and anthocyanins) accumulation, and antiviral activities. Results and conclusion The results indicated that 2,4-D alone (1.0 mg/l or 1.5 mg/l) induced callus formation most effectively. Furthermore, glucose was detected to be the more effective carbon source for the accumulation of chlorophylls, carotenoids, and anthocyanins compared with fructose and mannose. The highest accumulation of pigments was induced by 20 or 40 mg/l glucose supplements, with no significant difference between the effects of different concentrations. Unlike glucose, fructose or mannose did not enhance pigment accumulation; moreover, high concentrations had negative effects on pigment accumulation. Similarly, glucose was more suitable for the antiviral activity of E. milii extracts than fructose and mannose. The results will support the effective production of secondary metabolites and offer insights into the antiviral potency of E. milii.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"22 1","pages":"432 - 439"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42848886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunostimulatory and anti-inflammatory properties of Silene succulenta Forssk","authors":"Mohamed El Gendy, Hemaia Motawe, M. Maamoun, R. El-Akad, Ahmed Afifi","doi":"10.4103/epj.epj_121_22","DOIUrl":"https://doi.org/10.4103/epj.epj_121_22","url":null,"abstract":"Background and objectives Immunomodulation using medicinal plants is a useful alternative to classical conventional chemotherapy for several diseases, including cancer and autoimmune disorders. Therefore, the aim of this study was to investigate the immunomodulatory properties of Silene succulenta Forssk. extract that is a common herb growing in Egypt. Materials and methods S. succulenta was collected from Mediterranean coast region of Egypt, dried in shade, ground, and extracted using methanol. The effect of the plant extract on immune cells was tested using murine splenocytes and human peripheral blood mononuclear cells (PBMCs) proliferation colorimetric assays. A bioassay-directed fractionation was applied to identify the most active fraction and gas chromatography–mass spectrometry (GC–MS) was used to analyze it. The effect of the plant extract and its active fraction on human interleukin-2 (IL-2) was determined using enzyme-linked immunosorbent assay. Furthermore, the effect on macrophage nitric oxide production ability, macrophage viability, and HL-60-differentiated cells to neutrophil ROS production was tested. Results and conclusion Our results demonstrated that S. succulenta extract induced murine splenocytes and PBMCs proliferation in a concentration-dependent manner. Through bioassay-directed fractionation, we identified hexane subfraction as the active fraction of the extract and identified 18 compounds using GC–MS with major components of palmitic acid (33.19%) and phthalic acid, di(6-methylhept-2-yl) ester (20.22%). The extract and its active fraction induced human IL-2, whereas they inhibited macrophage nitrite-production ability, and neutrophil reactive oxygen species production level. We concluded that S. succulenta possesses several immunostimulatory and anti-inflammatory properties that can be utilized to treat several diseases.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"4 1","pages":"353 - 360"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139366191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effects of the Egyptian horned viper LAAO against hepatocellular carcinoma induced in rats","authors":"Gomaa Mahmoud, Samy Saber, Samah Loutfy, Walaa Salama, A. Nabeeh","doi":"10.4103/epj.epj_48_23","DOIUrl":"https://doi.org/10.4103/epj.epj_48_23","url":null,"abstract":"Background The most common kind of liver cancer, hepatocellular carcinoma (HCC), is the fourth leading cause of cancer-related mortality worldwide and has poor prognosis. Strong hepatocarcinogen diethyl nitrosamine (DENA) is a well-known substance. It is well known that DENA damages DNA repair enzymes and is typically used to cause liver cancer in experimental animal models, such as rats. Cerastes cerastes L-amino acid oxidase (Cc-LAAO) has hepatoprotective, antioxidant, and anticancer effects. Objective To assess the effectiveness of L-amino acid oxidase (LAAO) as a hepatoprotective agent in comparison to paclitaxel (PAC) as a conventional anticancer medicine in the early identification of HCC using biomarkers [alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA)], various liver function tests, and oxidant and antioxidant tests. Materials and methods CCl4 (200 mg/kg b.wt.) was injected subcutaneously once a week for 3 weeks after a single IP dose of DENA (200 mg/kg b.wt.) to develop hepatocellular cancer in rats. Twenty-five adult, mature, healthy rats were used in this investigation; their average weight was 100±10 g, and they were divided into five groups, each with five rats. After the experiment, some hepatic tests, histology of the liver, a tumor biomarker, and some kidney functions were assessed for all groups. Results and conclusion ASAT, ALAT, ALP, total bilirubin, tumor markers AFP, CEA, and lipid peroxides malondialdehyde (MDA) significantly rose in serum after DENA administration in rats, whereas activating antioxidants like SOD, CAT, GPx, and GSH decreased. LAAO and paclitaxel significantly ameliorated biomarkers for liver damage, lipid peroxides (MDA), antioxidants such as (SOD), (CAT), (GSH), (GPx), tumor marker (AFP), and (CEA) compared with the HCC group. Histopathology showed vacuolar hepatocytes with dispersed hepatocyte necrosis and infiltration of mononuclear cells. When used with DENA, the LAAO administration reduced negative effects and produced positive effects. These findings demonstrate that LAAO prevents liver HCC caused by DEN by preventing lipid peroxidation, hepatic cell oxidative stress, and boosting the antioxidant system.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"22 1","pages":"391 - 402"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41691340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous utilization of jasmonic acid and methyl jasmonate stimulates growth and biochemical composition of lavender (Lavandula angustifolia) plant","authors":"R. El-Ziat, Dina M. Soliman, Iman El-Sayed","doi":"10.4103/epj.epj_23_23","DOIUrl":"https://doi.org/10.4103/epj.epj_23_23","url":null,"abstract":"Background Lavender (Lavandula angustifolia) as an economic herb is used in traditional medicine, perfume, flavoring, and cosmetics. It is classified in the mint family (Lamiaceae) and commonly used as a landscape plant. Monoterpenes and sesquiterpenes are the most common chemical components found in essential oils. Because of these compounds, it is used for antifungal, antibacterial, and antioxidant activities. Secondary metabolite compounds, that is, jasmonic acid (JA) and methyl jasmonate (MeJA), significantly influence secondary metabolism regulation by stimulating the accumulation of phenols, flavonoids, and alkaloids. Objective The research aimed to estimate the effect of both JA and MeJA on growth parameters, chemical composition, particularly secondary metabolism, and the composition of volatile oils of L. angustifolia Mill plants. Materials and methods In the experiment, JA concentrations of 0, 1, 5, and 10 mM and MeJA concentrations of 0, 0.25, 0.50, and 1.0 mM were used as foliar spray applications on L. angustifolia. The investigation was performed as a randomized complete block design in three replicates. The parameters collected were different growth parameters, essential oil components using gas chromatography–mass spectrometry, the number of glands and secretory trichome diameter by scanning electron microscope, as well as chemical constituents. Results and conclusion The results showed that growth parameters were increased gradually with the increasing of JA and MeJA concentrations. The effects of JA and MeJA at high concentrations were more effective on all the growth parameters, biochemical components, and the number of glands and diameter of secretory trichomes than other concentrations and control plants in both seasons. JA application significantly increased chlorophyll a and b, carotenoids, and N and protein content of lavender plants. MeJA gave the highest values of total sugar, free amino acids, and phenols. Therefore, the results suggested that 10 mM of JA and 1.0 mM of MeJA significantly stimulate lavender plant growth and chemical compounds and volatile oil components, especially linalool and camphor, increasing its value and raising its quality in the perfume and cosmetics industries and various chemical industry applications.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"1 1","pages":"372 - 379"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139365769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticoagulant and antibacterial activities of polysaccharides fraction from Nostoc muscorum and Arthrospira platensis cultivated under abiotic stress conditions","authors":"Walaa Abd El Monsef, Awad Ragab, S. Shanab, Ghada Mahmoud, Emad Shalaby","doi":"10.4103/epj.epj_73_23","DOIUrl":"https://doi.org/10.4103/epj.epj_73_23","url":null,"abstract":"Background Cyanobacteria are very old phylogenetic group of prokaryotic phototrophic microorganisms. With repeated quick adaptations to different environmental circumstances in different ecosystems, their presence in the biosphere from the early Precambrian and extensive diversity up to the present witness to with their amazing and continuous physiologically and environmentally important life strategies. Polysaccharides have recently attracted a lot of attention their efficiency in various biological activities, including immunomodulatory, antibacterial, anticoagulant, antimutagenic, radioprotective, anti-oxidative, and anticancer. So, this research demonstrates the role of algal polysaccharides, produced under abiotic stress condition, in some of these biological activities. Objective The aim of this study was selected Nostoc muscorum and Arthrospira platensis as cyanobacteria species for studying the effect of some abiotic stress conditions (light intensity & sulfate concentrations and aeration rate) on polysaccharide production (intracellular and extracellular). Evaluate the ability of the different algal extracts as anticoagulants and antibacterial. Materials and methods N. muscorum was cultivated on BG-110 medium and A. platensis was cultivated on Zarrouk medium. Algal species were grown and incubated in controlled photoperiod of, 16-8 l/D cycles, light intensity of 40 µE/m2 /s and temperature at 25°C±2°C with continuous aeration supplied with air (60 bubbles/min.). Cultivation of algae under abiotic stress conditions and the growth rate of both investigated species was determined by optical density and dry cell weight. Extraction of polysaccharides by different methods and determination of total hydrolysable carbohydrates. Determination of sulfate contents. Protein molecular weight determination in algal species were determined by SDS-page. Determination of bacterial activity of different algal extracts, was tested in vitro against G+ and G- bacterial strains using the filter paper disk diffusion method, The Gram-positive bacterial strains, Staphylococcus aureus and the gram-negative strains, Pseudomonas aeruginosa, Also the anticoagulating activity of promising algal extracts was investigated using the method of US pharmacopeia. Results and conclusion There was positive correlation between light intensity, MgSO4 concentrations and aeration, on algal growth and polysaccharide production. Ethanol was found to be highly efficient for polysaccharide extraction. Intracellular polysaccharide content was higher than extracellularly released one in both the studied cyanobacteria species. Intracellular polysaccharides of A. platensis exhibited antibacterial activity higher than those of N. muscorum. Anticoagulation efficiency of sulfated polysaccharides in both investigated stressed cyanobacteria species was highly pronounced (>30 min). Chemically active groups showed by FT-IR of the produced polysaccharides under stressed conditions are variable d","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"22 1","pages":"516 - 534"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43592220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of inflammatory markers in egyptian obese and nonobese adolescents","authors":"A. El-Shaheed, Reham F. Fahmy, N. Mahfouz, Mona Elabd, H. Sibaii, S. El-Zayat","doi":"10.4103/epj.epj_29_23","DOIUrl":"https://doi.org/10.4103/epj.epj_29_23","url":null,"abstract":"Background Obesity causes an inflammation state that is related to co-morbidities such as hypertension and insulin resistance. Though, the underlying inflammatory events in obesity are still obscure particularly in pediatric age group. Objective To identify the initial stage of inflammation linked to this nutritional disorder. Materials and methods A case control observational study involved 45 cases of overweight/obese adolescents and 44 healthy peers of matching age and sex. Serum levels of Interleukin-10, Interleukin-18, and C − reactive protein were estimated. Leukocytes, Neutrophils, Lymphocytes counts were determined, and Neutrophil/ Lymphocyte Ratio was calculated. Results and conclusion C − reactive protein, Neutrophils, and Neutrophil/Lymphocyte Ratio were significantly higher in overweight/ obese adolescents (P = 0.000, P = 0.015, and P = 0.034, respectively). Interleukin-10 was non-significantly lower whereas IL-18 was non-significantly higher in overweight/ obese adolescents (P = 0.334, and P = 0.427, respectively). Obesity is accompanied by a low inflammatory state that exists since childhood. Therefore, timely interventions should be adopted to preclude the deleterious consequences in grown up adults.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"4 1","pages":"501 - 507"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139365591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of turmeric and carrot extracts on morphological, chemical composition and isozymes patterns of Azadirachta indica seedlings under water deficiency conditions","authors":"Amr Mohamed, Samah El-Sayed, Shaimaa I. M. Elsayed, A. Mazher","doi":"10.4103/epj.epj_41_23","DOIUrl":"https://doi.org/10.4103/epj.epj_41_23","url":null,"abstract":"Background Azadirachta indica trees are of great importance because of the high-quality wood they produce, which is used in a wide range of industries, and their production of insect repellent oils. Objective Due to these great benefits that have drawn attention to them, it was necessary to find environmentally friendly solutions to improve the ability of this plant species to grow in the event of exposure to conditions of water shortage. Materials and methods The plants were sprayed with Carrot extract (C) at rates of 50 and 100 ml/ l and/or Turmeric extract (T) at a rate of 20 and 40 ml/l under irrigation intervals every 4 and 8 days. Total chlorophyll content, total sugars content (mg/g F.W.), flavonoids content (mg/g F.W.), total phenols (mg/g F.W.) and total indoles (mg/100 g F.W.) were determined. Also, Peroxidase isozymes (POD) and Polyphenol oxidase isozymes (PPO) were determined. Results and conclusion The obtained results confirmed that increasing irrigation periods (8 days) had a negative effect on most vegetative traits except for all root characteristics, and also all chemical properties except the total indoles content and lipid peroxidation, it was also observed that the enzymatic activity of peroxidase isozymes (POD) and polyphenol oxidase isozymes (PPO) compared to plants that were watered every 4 days. The most of growth parameters, chemical composition and the activity of POD in plants irrigated every four days were improved with C treatment at 100 ml/l, while the activity of PPO increased with 100 ml/l C+40 ml/l T. On the other hand, all growth parameters, chemical compositions and POD in plants irrigated every 8 days were increased with 100 ml/l C +20 ml/l T except lipid peroxidation which gave the highest value in control plants, whereas PPO increased with C treatment 50 ml/l+T 40 ml/l.","PeriodicalId":11568,"journal":{"name":"Egyptian Pharmaceutical Journal","volume":"9 1","pages":"466 - 480"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139364532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}