宿主铁抑制策略对化脓性链球菌KSU-1株生长和致病性的影响

IF 0.7 Q4 PHARMACOLOGY & PHARMACY
M. Alkhulaifi, A. Al-Salamah, Eman Elgammal, A. Ibrahim
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引用次数: 0

摘要

背景和目的化脓性链球菌是一种适应性强的人类病原体,可引起从轻度到危及生命的全身性感染的广泛感染。本研究探讨了铁剥夺策略对化脓性链球菌生长、存活和毒力的影响。材料和方法比较不同铁饱和和铁贫化形式的主要人体铁库(血红蛋白、血红素、转铁蛋白、乳铁蛋白和母乳)对化脓性链球菌生长和致病性的影响。结果和结论尽管铁饱和形式提高了生长和存活率,但无铁形式对微生物具有抑菌/杀菌活性,这些结果在体内研究中得到了强调。最后,如十二烷基硫酸钠-聚丙烯酰胺凝胶电泳所示,细菌表面蛋白作为毒力因子在铁耗竭时分泌。本研究证明,铁缺乏环境增加了化脓性链球菌的抗性和毒力因子。此外,这些结果可以深入了解宿主和病原体之间的相互作用,从而设计出可能针对这些途径的新型抗菌剂或疫苗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Involvement of host iron-withholding strategy on Streptococcus pyogenes strain KSU-1 growth and pathogenicity
Background and objectives Streptococcus pyogenes is a highly adaptable human pathogen that can cause a wide spectrum of infections ranging from mild to a life-threating systemic infection. This study discussed the effectiveness of iron-depriving strategy on growth, survival, and virulence of S. pyogenes. Materials and methods Some comparisons between different iron-saturated and iron-depleted forms of the main human iron reservoirs (hemoglobin, hemin, transferrin, lactoferrin, and human milk) were tested for their effect on growth and pathogenicity of S. pyogenes. Results and conclusion Although the iron-saturated forms enhanced the growth and survival, the iron-free forms had a bacteriostatic/bactericidal activity against the microbe, and these results were emphasized by the in vivo study. Finally, the bacterial surface proteins as virulence factors were secreted upon iron depletion as indicated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. This study proved that iron-depletion environment increased the resistance and virulence factors of S. pyogenes. Moreover, these results can give an insight about the interaction between the host and pathogen, which can lead to designing of new antimicrobial agents or vaccine that may target these pathways.
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来源期刊
Egyptian Pharmaceutical Journal
Egyptian Pharmaceutical Journal PHARMACOLOGY & PHARMACY-
CiteScore
1.10
自引率
0.00%
发文量
37
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