Electronic Journal of Biotechnology最新文献

{"title":"Relationship between type II polyproline helix secondary structure and thermal hysteresis activity of short homopeptides","authors":"Roberto Rojas ,&nbsp;Mónica Aróstica ,&nbsp;Patricio Carvajal-Rondanelli ,&nbsp;Fernando Albericio ,&nbsp;Fanny Guzmán ,&nbsp;Constanza Cárdenas","doi":"10.1016/j.ejbt.2022.08.003","DOIUrl":"10.1016/j.ejbt.2022.08.003","url":null,"abstract":"<div><h3>Background</h3><p>Antifreezing activity is a phenomenon of great significance in food industry that affects the quality of frozen foods. As a solution, ice-binding proteins, more specifically antifreeze proteins, have been used to mitigate recrystallization. However, knowledge about the mechanism of ice recrystallization and the influence of antifreeze proteins is scarce.</p></div><div><h3>Results</h3><p>In this work, model homopeptides of three amino acids (proline, arginine and lysine) were studied by means of differential scanning calorimetry through the determination of their thermal hysteresis activity, to see the influence of several factors on their secondary structure. It was found that model homopeptides formed polyproline II type secondary structure that was more stable at low temperature. In addition, thermal hysteresis activity was higher for peptides of intermediate lengths and for proline homopeptides.</p></div><div><h3>Conclusions</h3><p>The study of homopeptides sheds light on the mechanism of antifreeze activity and will allow the design of new molecules with antifreeze properties to be used in diverse biotechnological fields.</p><p><strong>How to cite:</strong> Rojas R, Aróstica M, Carvajal-Rondanelli P, et al. Relationship between type II polyproline helix secondary structure and thermal hysteresis activity of short homopeptides. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.08.003.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"59 ","pages":"Pages 62-73"},"PeriodicalIF":2.7,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000331/pdfft?md5=07ecdc3423f06c584c59547a8c9e29f9&pid=1-s2.0-S0717345822000331-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42614951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of cellulase genes in Pseudomonas stutzeri","authors":"Naief H. Al Makishah ,&nbsp;Ameer E. Elfarash","doi":"10.1016/j.ejbt.2022.07.004","DOIUrl":"10.1016/j.ejbt.2022.07.004","url":null,"abstract":"<div><h3>Background</h3><p>Cellulose is one of the most abundant natural sources of carbon. In biofuel manufacturing, cellulase is used as an enzyme to hydrolyze cellulose into a fermentable product (glucose)<em>. Pseudomonas stutzeri</em> is one of the microorganisms found in cattle rumen. The microbiome of the rumen is heterogeneous and known for its potentiality to efficiently hydrolyze cellulose. Recent studies have identified, cloned, and crystallized one of the cellulase genes present in <em>P. stutzeri</em>, the A1501 cellulase gene (PST_2494 gene).</p></div><div><h3>Results</h3><p>This study describes the isolation of cellulase-producing bacteria from sheep's rumen. The highest cellulase-producing isolate was identified as <em>P. stutzeri</em> by 16s rDNA sequencing<em>.</em> qRT-PCR was used to measure the cellulase gene expression levels, revealing a higher gene expression of the PST_1459 gene (4 folds) compared to PST_2494 genes. Moreover, cellulase productivity was enhanced by UV irradiation mutagenesis.</p></div><div><h3>Conclusions</h3><p>Sheep's rumen bacterial isolates were tested for their cellulase productivity, and the highest was identified as <em>P. stutzeri</em>. An investigation of the cellulase genes of <em>P. stutzeri</em> revealed the presence of an unidentified cellulase gene (PST_1459). A qRT-PCR reaction was carried out to validate and measure the expression levels of different cellulase genes, revealing a higher gene expression of the PST_1459 gene than PST_2494 genes. Moreover, UV irradiation mutagenesis was performed to enhance cellulase productivity. The gene expression tested by qRT-PCR confirmed the enhancement of cellulase productivity in some of the mutants obtained.</p><p><strong>How to cite:</strong> Al Makishah NH, Elfarash AE. Molecular characterization of cellulase genes in <em>Pseudomonas stutzeri</em>. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.07.004.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"59 ","pages":"Pages 55-61"},"PeriodicalIF":2.7,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000306/pdfft?md5=e246c9613fb6975ebec857eb2e8ff84f&pid=1-s2.0-S0717345822000306-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46172163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lignosus rhinocerus attenuates non-alcoholic fatty liver induced by plant-based high-fat diet in hamster","authors":"Wen-Che Tsai ,&nbsp;Teck-Loong Hoe ,&nbsp;Shao-Chun Lu ,&nbsp;Tzu-Chien Kao ,&nbsp;Hsin-Yi Yin ,&nbsp;Jiunn-Wang Liao ,&nbsp;Hsiao-Wei Wen","doi":"10.1016/j.ejbt.2022.05.004","DOIUrl":"10.1016/j.ejbt.2022.05.004","url":null,"abstract":"<div><h3>Background</h3><p>Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder globally, and is often caused by abnormal fatty acid metabolism. Plant-based fatty acid may lower the risk of NAFLD, but evidence shows that plant-based fatty acid can still induce fatty liver. <em>Lignosus rhinoceros</em> is a mushroom that is widely used as folk medicine in Southeast Asia. Studies indicate that <em>L. rhinoceros</em> can act as an antioxidant and immune modulator, might be a potent modulator for NAFLD. In this study, a plant-based high-fat diet (HFD) was used to induce fatty liver in hamsters. Hot water extract of <em>L. rhinoceros</em> was applied during the induction period. The antioxidant capacity of <em>L. rhinoceros</em> was evaluated.</p></div><div><h3>Results</h3><p>Data reveal that <em>L. rhinoceros</em> extract had promising DPPH scavenging and ferrous ion chelating compacities. The total phenolic and flavonoid contents were 366.23 ± 5.06 mg gallic acid and 28.67 ± 2.5 mg rutin equivalents per gram of raw <em>L. rhinocerus</em> powder, respectively. <em>Lignosus rhinoceros</em> extract lowered body weight, and reduced liver damage which was induced by HFD in hamster, possibly by reducing mononuclear cell infiltration in the liver. <em>Lignosus rhinoceros</em> treatment also lowered the cholesterol content and improved the HDL-c/LDL-c level in hamster serum. Finally, <em>L. rhinoceros</em> reduced the reactive oxygen species content in the liver which was caused by HFD.</p></div><div><h3>Conclusions</h3><p>This investigation provides evidence that a plant-based HFD may be a risk factor in fatty liver. It also proves that <em>L. rhinoceros</em> promotes liver health, and so can be used in functional food.</p><p><strong>How to cite:</strong> Tsai W-C, Hoe T-L, Lu S-C, et al. <em>Lignosus rhinocerus</em> attenuates non-alcoholic fatty liver induced by plant-based high-fat diet in hamster. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.05.004.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 46-54"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000215/pdfft?md5=781688541af6dfb0d7ba2bed04c2c40f&pid=1-s2.0-S0717345822000215-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46518958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved laccase production in Pleurotus djamor RP by atmospheric and room temperature plasma (ARTP) mutagenesis","authors":"Zichen Zhang ,&nbsp;Aabid Manzoor Shah ,&nbsp;Hassan Mohamed ,&nbsp;Yao Zhang ,&nbsp;Beenish Sadaqat ,&nbsp;Nino Tsiklauri ,&nbsp;Tinatin Sadunishvili ,&nbsp;Yuanda Song","doi":"10.1016/j.ejbt.2022.03.005","DOIUrl":"10.1016/j.ejbt.2022.03.005","url":null,"abstract":"<div><h3>Background</h3><p><em>Pleurotus djamor</em> is an edible mushroom with medicinal properties, and degrades lignocellulose by producing enzymes that have a variety of industrial applications. The aim of this study was to improve laccase producing potential of <em>P. djamor</em> that may easily convert lignocellulose waste into high-value products.</p></div><div><h3>Results</h3><p>In the present study, protoplast of the newly isolated fungus <em>P. djamor</em> RP was mutated by atmospheric pressure and room temperature plasma (ARTP) method. Guaiacol was used as an indicator for the rapid visual appearance of laccase positives in the screening process. From the results of preliminary screening, 10 promising positive mutants with a maximum laccase production were selected for further experimentation. Lac activity by the ABTS method showed that the mutant <em>P. djamor</em> strain 51-4 exhibited the maximum laccase production of 494.44 U/L in shake flask cultivation, which was 86.36% higher than wild strain. Notably, the other mutant <em>P. djamor</em> strain 62-27 exhibits a higher laccase production of 26.06 U/g which was 30.28% higher than wild strain when grown on solid-state fermentation. In addition to a higher yield of laccase, all mutants showed better growth and genetic stability than the wild strain.</p></div><div><h3>Conclusions</h3><p><em>P. djamor</em> RP mutants with high laccase-producing potential were developed by ARTP Mutagenesis technique, and these isolates could be used as a possible candidate for lignocellulose conversion into high-value products.</p><p><strong>How to cite</strong>: Zhang Z, Shah AM, Mohamed H, et al. Improved laccase production in <em>Pleurotus djamor</em> RP by atmospheric and room temperature plasma (ARTP) mutagenesis. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.03.005</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 1-9"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000112/pdfft?md5=4b23e1cad962a9fe9ba2995f1e234f58&pid=1-s2.0-S0717345822000112-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43061258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A meta-analysis of low temperature tolerance QTL in maize","authors":"Tao Yu,&nbsp;Jianguo Zhang,&nbsp;Jingsheng Cao,&nbsp;Shiliang Cao,&nbsp;Wenyue Li,&nbsp;Gengbin Yang","doi":"10.1016/j.ejbt.2022.05.002","DOIUrl":"10.1016/j.ejbt.2022.05.002","url":null,"abstract":"<div><h3>Background</h3><p>Cold injury is one of the most important limiting factors for maize production in mid-high latitude regions in the world. A total of 314 QTLs for maize low temperature tolerance have been identified in different populations using different statistical methods. However, few identical QTLs have been identified in different research studies.</p></div><div><h3>Results</h3><p>A consensus map of QTLs related to maize low temperature tolerance was constructed, based on the public genetic map, IBM2 2008 Neighbors as a reference map, along with a set of 314 QTLs reported in the literature over the past 20 years. A total of 187 QTLs were projected onto the IBM2 2008 Neighbors by software BioMercator. Forty-seven consensus QTLs were detected. The confidence interval at all sites ranged from 0.04 cM to 102.73 cM, and the proportion of the phenotypic variance associated with each of them ranged from 3.32% to 20.11%. Major chromosomal sites were identified on Chr.6 (MQTL29, MQTL30, and MQTL31).</p></div><div><h3>Conclusions</h3><p>This study provides further insights into the genetic basis of maize low temperature tolerance. Moreover, the MQTLs reported here could be harnessed for functional marker development and candidate gene mining of maize low temperature tolerance.</p><p><strong>How to cite:</strong> Yu T, Zhang J, Cao J, et al. A meta-analysis of low temperature tolerance QTL in maize. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.05.002</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 82-91"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000197/pdfft?md5=c63f4414cacd3512b28f6f290cd91ee6&pid=1-s2.0-S0717345822000197-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48753705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple-objective optimization of lactic-fermentation parameters to obtain a functional-beverage candidate","authors":"Paola M. Alvarado-Cóndor,&nbsp;Jimmy Núñez-Pérez,&nbsp;Rosario C. Espín-Valladares,&nbsp;José M. Pais-Chanfrau","doi":"10.1016/j.ejbt.2022.04.001","DOIUrl":"10.1016/j.ejbt.2022.04.001","url":null,"abstract":"<div><h3>Background</h3><p>Whey is the most abundant by-product of the cheese industry. It is estimated that it contains up to 55% of all nutrients of milk and therefore, it is considered a starting material for obtaining valuable products.</p></div><div><h3>Results</h3><p>The response surface methodology was used to find the combination of temperature (between 20 and 36°C), and the content of whey powder (37.5–77% (m/m)) to maximize the concentration of kefiran, the concentration of lactic acid bacteria (LAB) and yeast in the supernatant. After validating the quadratic models of each transformed response variable, it underwent a maximization procedure to find the optimal condition obtaining two maximum spaces at the temperature range of 28.5–29.7°C and 43.3% (m/m) of whey-powder content, or 28.0–28.3°C and 71.2% (m/m) of whey-powder content. The validation experiments were carried out for the first suggested optimal solution, through three repetitions under the same optimal conditions, and it was confirmed that there is no significant difference with the values provided by the model.</p></div><div><h3>Conclusions</h3><p>Physicochemical characteristics (protein, fat, acidity, lactose, viscosity, alcoholic content) under optimal conditions were evaluated and proved its compliance with the Ecuadorian and Andean community regulations. These results suggest that we are in the presence of a functional beverage candidate in which the contents of LAB and yeast (probiotics) and kefiran (prebiotic) were simultaneously maximized.</p><p><strong>How to cite:</strong> Alvarado-Cóndor PM, Núñez-Pérez J, Espín-Valladares RC, et al. Multiple-objective optimization of lactic-fermentation parameters to obtain a functional-beverage candidate. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.04.001</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 10-13"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000124/pdfft?md5=61eb39271bf4485c8b092e6b126c226c&pid=1-s2.0-S0717345822000124-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44084877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA-seq analysis and transcriptome assembly of Salicornia neei reveals a powerful system for ammonium detoxification","authors":"Mónica Díaz-Silva ,&nbsp;Jonathan Maldonado ,&nbsp;Pamela Veloso ,&nbsp;Nicol Delgado ,&nbsp;Herman Silva ,&nbsp;José A. Gallardo","doi":"10.1016/j.ejbt.2022.05.003","DOIUrl":"https://doi.org/10.1016/j.ejbt.2022.05.003","url":null,"abstract":"<div><h3>Background</h3><p><em>Salicornia neei</em> is a halophyte plant that has been proposed for use in the phytoremediation of the saline wastewater generated by land-based aquaculture. To identify the molecular mechanisms related to ammonium response, we analyzed the transcriptome of <em>S. neei</em> in response to growth in saline water containing 3 mM ammonium.</p></div><div><h3>Results</h3><p>The RNA sequencing generated a total of 14,680,108 paired-end reads from the control and stressed conditions. De novo assembly using the CLC Genomic Workbench produced 86,020 transcripts and a reference transcriptome with an N50 of 683 base pair.</p><p>A total of 45,327 genes were annotated, representing 51.2% of the contig predicted from de novo assembly. As regards differentially expressed genes, a total of 9,140 genes were differentially expressed in response to ammonium in saline water; of these, 7,396 could be annotated against functional databases. The upregulated genes were mainly involved in cell wall biosynthesis, transmembrane transport and antiporter activities, including biological Kyoto Encyclopedia of Genes and Genomes, pathways linked to the biosynthesis of secondary metabolites, plant hormone signal transduction, autophagy, and nitrogen metabolism. In addition, a set of 72 genes was directly involved in ammonium metabolism, including glutamine synthetase 1, glutamate synthase 1, and ferredoxin-dependent glutamate synthase chloroplastic.</p></div><div><h3>Conclusions</h3><p>Our results support the hypothesis that an ammonium detoxification system mediated by glutamine and glutamate synthase was activated in <em>S. neei</em> when exposed to ammonium and saline water. The present transcriptome profiling method could be useful when investigating the response of halophyte plants to saline wastewater from land-based aquaculture.</p><p><strong>This manuscript includes an interactive 360 degree video</strong>, supplementary to the materials and methods section. To view the video correctly, it is necessary to scroll through the screen to navigate across the laboratory where you will find 6 interactive points. For an immersive experience a head-mounted display can be used. <strong>Please, visit this URL:</strong> <span>http://ejbiotechnology.info/public/360view/2022/VTPGALLARDO_1v3/index.htm</span><svg><path></path></svg>.</p><p><strong>How to cite:</strong> Díaz-Silva M, Maldonado J, Veloso P, et al. RNA-seq analysis and transcriptome assembly of <em>Salicornia neei</em> reveals a powerful system for ammonium detoxification. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.05.003</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 70-81"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000203/pdfft?md5=f27b8fa0d20e34dd8ad7611e59dbf5d7&pid=1-s2.0-S0717345822000203-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137371699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal transcriptome analysis provides molecular insights into flower development in red-flesh pitaya","authors":"Zhijiang Wu ,&nbsp;Lifang Huang ,&nbsp;Fengzhu Huang,&nbsp;Guifeng Lu,&nbsp;Shuotong Wei,&nbsp;Chaoan Liu,&nbsp;Haiyan Deng,&nbsp;Guidong Liang","doi":"10.1016/j.ejbt.2022.05.005","DOIUrl":"10.1016/j.ejbt.2022.05.005","url":null,"abstract":"<div><h3>Background</h3><p>Pitaya is an important economic fruit worldwide due to its numerous health benefits. A systematic understanding of the mechanism underlying flower development is essential to obtain higher fruit yield and quality. However, the genetic mechanism of flower development is not yet investigated in pitaya. Herein, a transcriptome analysis was performed to determine the transcriptional changes during flower development in red-flesh pitaya by utilizing nine different stages of flower development.</p></div><div><h3>Result</h3><p>A total of 95,412 unigenes were generated with a mean length of 913 nt, and N50 value of 1878 nt. Comparative transcriptomic analysis showed many differentially expressed genes (DEGs) among the flower growth stages. Furthermore, an array of key DEGs were enriched in hormone signaling, transcription, carbohydrate transport, and energy production pathways. In particular, indole-3-acetic acid, abscisic acid, ethylene-responsive transcription factor, constans-like, teosinte branched 1/cycloidea/proliferating cell nuclear antigen factor, apetala1-like, agamous-like MADS-box protein, sepallata, growth-regulating factor, putative axial regulator yabby, leafy/floricaula homolog, and <em>MYB</em> gene-associated transcription factors displayed altered expression, suggesting their critical roles in floral organ development of pitaya. Besides, genes related to sugar synthesis, transportation, and utilization mediate flower growth regulation in pitaya. Eleven genes were selected to perform qRT-PCR analysis to verify the results of the RNA sequencing.</p></div><div><h3>Conclusions</h3><p>Our results provide important insights into the transcriptional regulation of pitaya flower development. These data resources will be a cornerstone for future research that aims at exploring the genetic control of flower development in pitaya.</p><p><strong>How to cite:</strong> Wu Z, Huang L, Huang F, et al. Temporal transcriptome analysis provides molecular insights into flower development in red-flesh pitaya. Electron J Biotechnol 2022;58 https://doi.org/10.1016/j.ejbt.2022.05.005</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 55-69"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000227/pdfft?md5=8a3eda6869111f20e14efc9da2ab7527&pid=1-s2.0-S0717345822000227-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44201856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radical scavenging potency, HPLC profiling and phylogenetic analysis of endophytic fungi isolated from selected medicinal plants of Saudi Arabia","authors":"Abdallah M.A. Hassane ,&nbsp;Taher M. Taha ,&nbsp;Mohamed F. Awad ,&nbsp;Hassan Mohamed ,&nbsp;Mohammad Melebari","doi":"10.1016/j.ejbt.2022.05.001","DOIUrl":"10.1016/j.ejbt.2022.05.001","url":null,"abstract":"<div><h3>Background</h3><p>Endophytic fungi are associated within plant tissues without any harmful effects. They produce a diversity of metabolites and are well known as rich source of bioactive substances. This study aimed to identify fungal isolates from different medicinal plants, evaluate their antioxidant potency and detect components responsible for antioxidant activity.</p></div><div><h3>Results</h3><p>In the present investigation, 4 endophytic fungal isolates were obtained from different medicinal plants <em>Rumex nervosus</em>, <em>Pulicaria crispa</em> and <em>Withania somnifera</em>. Those isolates were characterized as <em>Penicilluim commune, Penicilluim glaucoroseum, Aspergillus flavipes</em> and <em>Fusarium chlamydosporum</em> according to morphological and phylogenetic bases<em>.</em> These strains were propagated on humified rice and their crude ethyl acetate extracts were assayed for their total phenols, flavonoids and antioxidant potency against DPPH free radicals. The results showed that the elevated constituent of phenolics was found in <em>P. commune</em> (148.24 mg/g), followed by <em>F. chlamydosporum</em> (124.25 mg/g), while the highest total flavonoids were determined in <em>A. flavipes</em>, followed by <em>F. chlamydosporum, P. glaucoroseum, P. commune,</em> respectively. Moreover, the maximum hydroxyl radical-scavenging assay was IC₅₀ = 2.13 mg/mL exhibited by <em>P. commune</em>, followed by <em>F. chlamydosporum</em> (2.68 mg/mL). Weak antimicrobial and cytotoxic effects were reported by the studied strains against pathogenic isolates and (HepG2) hepatocellular carcinoma cell line. The fungal EtOAc extracts were characterized quantitatively by the incidence of different phenols, flavonoids, and phenolic acids, as detected by HPLC.</p></div><div><h3>Conclusions</h3><p>Endophytic fungi derived from medicinal plants are a promising candidate for the wide industrial production of antioxidant agents with several useful medical and pharmaceutical applications.</p><p><strong>How to cite:</strong> Hassane A. M. A, Taha T. M, Awad M. F, et al. Radical scavenging potency, HPLC profiling and phylogenetic analysis of endophytic fungi isolated from selected medicinal plants of Saudi Arabia. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.05.001</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 37-45"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000185/pdfft?md5=4f851b7954ea67ddfa97583a1c086101&pid=1-s2.0-S0717345822000185-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43360355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of miR-141-3p over-expression in ovarian cancer","authors":"Lin Shi ,&nbsp;Hao-Jia Sun ,&nbsp;Jing-Jing Zeng ,&nbsp;Zi-Qian Liang ,&nbsp;Yun-Hua Lin ,&nbsp;Su-Ning Huang ,&nbsp;Jiang-Hui Zeng ,&nbsp;Li Yang ,&nbsp;Hao Chen ,&nbsp;Jie Luo ,&nbsp;Kang-Lai Wei","doi":"10.1016/j.ejbt.2022.04.006","DOIUrl":"10.1016/j.ejbt.2022.04.006","url":null,"abstract":"<div><h3>Background</h3><p>The expression of miR-141-3p in many malignant tumors has been verified. Nevertheless, the relationship between ovarian cancer and miR-141-3p remains undetermined. Therefore, further exploration is required.</p></div><div><h3>Results</h3><p>According to data from 100 samples, the final results of RT-qPCR showed that miR-141-3p was highly expressed in ovarian cancer. Furthermore, miR-141-3p was able to distinguish ovarian cancer cells from ovary tissues. The most significant Kyoto Encyclopedia of Genes and Genomes pathway, was regulation of lipolysis in adipocytes in ovarian cancer. The expression of PIK3R1 was negatively correlated with miR-141- 3p. PIK3R1 has a combing site with miR-141-3p.</p></div><div><h3>Conclusions</h3><p>This study examined the expression levels and mechanism of miR-141-3p in ovarian cancer for the first time. The results suggested that miR-141-3p may promote the occurrence of ovarian cancer by down-regulating PIK3R1.</p><p><strong>How to cite:</strong> Shi L, Sun H-J, Zeng J-J, et al. Evaluation of miR-141-3p over-expression in ovarian cancer. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.04.006</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 14-24"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000173/pdfft?md5=d2d49ce0b2037396953cc02d7dee69ea&pid=1-s2.0-S0717345822000173-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41608766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}