Electronic Journal of Biotechnology最新文献

{"title":"A case study of a profitable mid-tech greenhouse for the sustainable production of tomato, using a biofertilizer and a biofungicide","authors":"Leobardo Serrano-Carreón ,&nbsp;Sergio Aranda-Ocampo ,&nbsp;Karina A. Balderas-Ruíz ,&nbsp;Antonio M. Juárez ,&nbsp;Edibel Leyva ,&nbsp;Mauricio A. Trujillo-Roldán ,&nbsp;Norma A. Valdez-Cruz ,&nbsp;Enrique Galindo","doi":"10.1016/j.ejbt.2022.06.003","DOIUrl":"10.1016/j.ejbt.2022.06.003","url":null,"abstract":"<div><h3>Background</h3><p>Protected agriculture (PA) is an alternative allowing the control of environmental variables to produce healthy vegetables. Biofertilizers and biofungicides can reduce the chemical load of pesticides. There is abundant literature documenting individual aspects, such as control of environmental variables, irrigation, biological control, and cost assessments. However, there are no reports documenting integral approaches in which variables are considered altogether in a successful case study of mid-tech technology, suitable in middle-income countries like México. We tested if mid-tech greenhouses using biocontrol and biofertilization can increase profits, using tomato as a model system. This work provides considerations about middle-income countries’ agriculture and the need for a multidisciplinary approach to offer cost-effective, sustainable alternatives to producers.</p></div><div><h3>Results</h3><p>This technology yielded up to 254 tons/ha·year of tomato, achieving reductions of 44–60% in water consumption, 25% in chemical nitrogen-fertilization, and 28% in the cost unit of production, increasing the profits by ∼45% in relation to Mexican conventional greenhouses management.</p></div><div><h3>Conclusions</h3><p>This case study has shown that it is possible to significantly increase profits in mid-tech greenhouse tomato production by increasing productivity and crop quality and decreasing the use of water and agrochemicals through greenhouse automatization, crop management, and beneficial bacteria applied to crops<strong>.</strong> <strong>This manuscript includes a video</strong>, supplementary to the main contributions of the project. <strong>Please visit this URL:</strong> <span>https://youtu.be/uRBGgJqfkLE</span><svg><path></path></svg>.</p><p><strong>How to cite:</strong> Serrano-Carreón L, Aranda-Ocampo S, Balderas-Ruíz KA, et al. A case study of a profitable mid-tech greenhouse for the sustainable production of tomato, using a biofertilizer and a biofungicide. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.06.003.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"59 ","pages":"Pages 13-24"},"PeriodicalIF":2.7,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000252/pdfft?md5=e065ab8b270c9392f2b02b1c21188c01&pid=1-s2.0-S0717345822000252-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43873010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmid pND6-1 enhances the stability and conjugative transfer of co-resident companion plasmid pND6-2 in the naphthalene-degradative Pseudomonas putida strain ND6","authors":"Shan Wang ,&nbsp;Mingming Liu ,&nbsp;Dan Wang ,&nbsp;Shanshan Li ,&nbsp;Wei Yan","doi":"10.1016/j.ejbt.2022.08.002","DOIUrl":"10.1016/j.ejbt.2022.08.002","url":null,"abstract":"<div><h3>Background</h3><p>Bacterial cells often harbor multiple plasmids, but the interaction between these plasmids is poorly understood. <em>Pseudomonas putida</em> ND6, one of the typical naphthalene-degrading bacteria, contains two annotated plasmids, pND6-1 and pND6-2. The conjugative plasmid pND6-2 can be used as a helper to mobilize the naphthalene-degrading plasmid pND6-1. The role of pND6-1 in functions other than naphthalene degradation has never been explored. Therefore, it is of great significance to study the possible effect of pND6-1 on the transfer rate or maintenance of pND6-2.</p></div><div><h3>Results</h3><p>To investigate the interaction between pND6-1 and pND6-2, we successfully cured plasmid pND6-1 using the incompatibility method, generated the pND6-1-null strain ND6dp1, and first classified it into the IncP-7 group by curing experimental evidence. Subsequently, the pND6-1-cured mutant showed an insignificant increase in cell growth compared with the wild ND6, indicating that a lower cost was imposed on the host by pND6-1. The influences of pND6-1 on the stability and conjugation of plasmid pND6-2 were subsequently explored. The results showed that the presence of pND6-1 enhanced the stability of pND6-2 in natural host ND6 and heterologous host <em>P. putida</em> KT2440. In addition, the presence of pND6-1 in wild-type hosts promoted intra-specific and inter-specific conjugative transfer efficiency of pND6-2 by 3.9–6.3-fold with <em>P. putida</em>, <em>P. resinovans,</em> and <em>P. fluorescens</em> as the recipients, respectively.</p></div><div><h3>Conclusions</h3><p>Collectively, these results suggest that the plasmid pND6-1 improves the pND6-2 plasmid's stability and transfer rate in the ND6 strain with the selected recipient strains.</p><p><strong>How to cite:</strong> Wang S, Liu M, Wang D, et al. Plasmid pND6-1 enhances the stability and conjugative transfer of co-resident companion plasmid pND6-2 in the naphthalene-degradative <em>Pseudomonas putida</em> strain ND6. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.08.002.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"59 ","pages":"Pages 74-82"},"PeriodicalIF":2.7,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000318/pdfft?md5=8969336558eb5397fa5000bf12a1e737&pid=1-s2.0-S0717345822000318-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44683733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum-isolated exosomes from Piscirickettsia salmonis-infected Salmo salar specimens enclose bacterial DnaK, DnaJ and GrpE chaperones","authors":"Cristián Muñoz ,&nbsp;Marisela Carmona ,&nbsp;Omar Luna ,&nbsp;Fernando A. Gómez ,&nbsp;Constanza Cárdenas ,&nbsp;Patricio Flores-Herrera ,&nbsp;Rodrigo Belmonte ,&nbsp;Sergio H. Marshall","doi":"10.1016/j.ejbt.2022.07.003","DOIUrl":"10.1016/j.ejbt.2022.07.003","url":null,"abstract":"<div><h3>Background</h3><p>Endosomally produced by eukaryotic cells, exosomes are microvesicles involved in cell-to-cell communication. Exosomes have shown a wide range of therapeutic potential as a drug or vaccine delivery system, and they are useful as biomarkers in several disease processes. Another biological function described is pathogen dissemination through host-derived molecules released during infection, thus modulating the immune response in the host.</p></div><div><h3>Results</h3><p>This work characterizes the exosomal fraction recovered from serum of <em>Piscirickttesia salmonis</em>-challenged <em>Salmo salar</em> specimens and from the corresponding non-challenged controls. Exosomes presented a spherical morphology and particle size distribution within 50–125 nm, showing similar parameters in both groups. The mass spectrometry analysis of exosomes isolated at 14 and 21 d post-challenge showed the presence of peptides corresponding to the three proteins of Hsp70/DnaK chaperone system (DnaK, DnaJ, and GrpE). BLAST search of these peptides showed the specificity to <em>P. salmonis</em>. Data are available via ProteomeXchange with identifier PXD023594.</p></div><div><h3>Conclusions</h3><p>The chaperones were found with &gt;95% identity in the core genome when aligned to 73 genomes of <em>P. salmonis.</em> The proteins also showed a high degree of similarity with other microorganisms, where this system has proven to be vital for their survival under stress conditions.</p><p>The presence of these three proteins in exosomes isolated from challenged fish sera calls for further study into their potential role in bacterium pathogenicity.</p><p><strong>How to cite:</strong> Muñoz C, Carmona M, Luna O, et al. Serum-isolated exosomes from <em>Piscirickettsia salmonis-</em>infected <em>Salmo salar</em> specimens enclose bacterial DnaK, DnaJ and GrpE chaperones. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.07.003.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"59 ","pages":"Pages 83-93"},"PeriodicalIF":2.7,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S071734582200029X/pdfft?md5=928244b1a5d7b4998149d7905beb275f&pid=1-s2.0-S071734582200029X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46768882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction temperature impacts the structure of recombinant HuGM-CSF inclusion bodies in thermoinducible E. coli","authors":"Sara Restrepo-Pineda ,&nbsp;Diego Rosiles-Becerril ,&nbsp;Angélica B. Vargas-Castillo ,&nbsp;Luis P. Ávila-Barrientos ,&nbsp;Axel Luviano ,&nbsp;Nuria Sánchez-Puig ,&nbsp;Enrique García-Hernández ,&nbsp;Nestor O. Pérez ,&nbsp;Mauricio A. Trujillo-Roldán ,&nbsp;Norma A. Valdez-Cruz","doi":"10.1016/j.ejbt.2022.08.004","DOIUrl":"10.1016/j.ejbt.2022.08.004","url":null,"abstract":"<div><h3>Background</h3><p>The temperature upshift has been widely used as an induction system to produce recombinant proteins (RPs). However, thermoinduction could affect bacterial metabolism, RP production, and RP aggregation. Understanding the structure and functionality of those aggregates, known as inclusion bodies (IBs), is a research area of interest in bioprocesses being scarcely studied under thermoinduction. Here, we describe the effect of the thermoinduction (39°C or 42°C) on the production of the recombinant human granulocyte–macrophage colony-stimulating factor (rHuGM-CSF) using <em>Escherichia coli</em> W3110 under the system λ<em>pL</em>/cI857.</p></div><div><h3>Results</h3><p>Results indicated that at 39°C, the production of biomass was almost doubled as well as the acetate accumulation compared to 42°C. Cultures thermoinduced at 42°C improved 1.5-fold the total protein over biomass yield and 1.25-fold the RP over total protein yield. Furthermore, 42°C accelerated the onset of IB formation, changing its architecture. Additionally, IBs formed at 42°C were less soluble and presented higher disorderly structures compared with IBs formed at 39°C, enriched in α-helix and amyloidal-like structures.</p></div><div><h3>Conclusions</h3><p>This study highlights the observation that IBs attain different architecture in response to small changes in environmental conditions, such as the induction temperature, being this helpful information to improve thermoinduced bioprocesses.</p><p><strong>How to cite:</strong> Restrepo-Pineda S, Rosiles-Becerril D, Vargas-Castillo AB, et al. Induction temperature impacts the structure of recombinant HuGM-CSF inclusion bodies in thermoinducible <em>E. coli</em>. Electron J Biotechnol 2022;59. <span>https://doi.org/10.1016/j.ejbt.2022.08.004</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"59 ","pages":"Pages 94-106"},"PeriodicalIF":2.7,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000343/pdfft?md5=6fd66ff723f26b59c3dee0825564507c&pid=1-s2.0-S0717345822000343-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43557411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sheep IGFBP2 and IGFBP4 promoter methylation regulates gene expression and hair follicle development","authors":"Yuezhen Tian ,&nbsp;Jianwen Du ,&nbsp;Xuemei Yang ,&nbsp;Weidan Zeng ,&nbsp;Junmin He ,&nbsp;Bingru Zhao ,&nbsp;Xuefeng Fu ,&nbsp;Xinming Xu ,&nbsp;Weiwei Wu ,&nbsp;Jiang Di ,&nbsp;Xixia Huang ,&nbsp;Kechuan Tian","doi":"10.1016/j.ejbt.2022.07.001","DOIUrl":"10.1016/j.ejbt.2022.07.001","url":null,"abstract":"<div><h3>Background</h3><p>Hair follicle development is important for the fineness of wool. Our previous methylated DNA immunoprecipitation sequencing showed that <em>IGFBP2</em> and <em>IGFBP4</em> were differentially methylated at different development stages of the hair follicle of Super Merino sheep. This study selected these two genes as candidates to uncover the influence of their promoter methylation on hair follicle development.</p></div><div><h3>Results</h3><p>The results showed that the expression levels of <em>IGFBP2</em> and <em>IGFBP4</em> in the skin of very fine wool Super Merino sheep were higher than that in the very thick wool group, and the difference for <em>IGFBP4</em> was significant. The total methylation rate of <em>IGFBP2</em> promoter was extremely low, and no significant difference was found in the skin tissue of the Super Merino sheep at different fineness groups. However, the total methylation rate of <em>IGFBP4</em> promoter in the very thick group was significantly higher than that in the very fine group, and the total DNA methylation levels of the <em>IGFBP4</em> were significantly negatively correlated with mRNA expression. Furthermore, the transcription factor prediction results showed 104 and 91 candidate binding sites for transcription factors in the promoter regions of <em>IGFBP2</em> and <em>IGFBP4</em>, respectively. Among these, JUN, EP300, SP1, PAX6, ETS1, and MYOD1 may be related to hair follicle development.</p></div><div><h3>Conclusions</h3><p>The total methylation level of <em>IGFBP4</em> promoter was negatively correlated with its mRNA expression in Super Merino sheep skin tissues with different degrees of fineness, which could be used as an epigenetic marker for wool breeding in Super Merino sheep.</p><p><strong>How to cite:</strong> Tian Y, Du J, Yang X, et al. Sheep <em>IGFBP2</em> and <em>IGFBP4</em> promoter methylation regulates gene expression and hair follicle development. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.07.001.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"59 ","pages":"Pages 46-54"},"PeriodicalIF":2.7,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000276/pdfft?md5=73e08eec2be897991ff6de4818dffea1&pid=1-s2.0-S0717345822000276-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47673156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between type II polyproline helix secondary structure and thermal hysteresis activity of short homopeptides","authors":"Roberto Rojas ,&nbsp;Mónica Aróstica ,&nbsp;Patricio Carvajal-Rondanelli ,&nbsp;Fernando Albericio ,&nbsp;Fanny Guzmán ,&nbsp;Constanza Cárdenas","doi":"10.1016/j.ejbt.2022.08.003","DOIUrl":"10.1016/j.ejbt.2022.08.003","url":null,"abstract":"<div><h3>Background</h3><p>Antifreezing activity is a phenomenon of great significance in food industry that affects the quality of frozen foods. As a solution, ice-binding proteins, more specifically antifreeze proteins, have been used to mitigate recrystallization. However, knowledge about the mechanism of ice recrystallization and the influence of antifreeze proteins is scarce.</p></div><div><h3>Results</h3><p>In this work, model homopeptides of three amino acids (proline, arginine and lysine) were studied by means of differential scanning calorimetry through the determination of their thermal hysteresis activity, to see the influence of several factors on their secondary structure. It was found that model homopeptides formed polyproline II type secondary structure that was more stable at low temperature. In addition, thermal hysteresis activity was higher for peptides of intermediate lengths and for proline homopeptides.</p></div><div><h3>Conclusions</h3><p>The study of homopeptides sheds light on the mechanism of antifreeze activity and will allow the design of new molecules with antifreeze properties to be used in diverse biotechnological fields.</p><p><strong>How to cite:</strong> Rojas R, Aróstica M, Carvajal-Rondanelli P, et al. Relationship between type II polyproline helix secondary structure and thermal hysteresis activity of short homopeptides. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.08.003.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"59 ","pages":"Pages 62-73"},"PeriodicalIF":2.7,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000331/pdfft?md5=07ecdc3423f06c584c59547a8c9e29f9&pid=1-s2.0-S0717345822000331-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42614951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of cellulase genes in Pseudomonas stutzeri","authors":"Naief H. Al Makishah ,&nbsp;Ameer E. Elfarash","doi":"10.1016/j.ejbt.2022.07.004","DOIUrl":"10.1016/j.ejbt.2022.07.004","url":null,"abstract":"<div><h3>Background</h3><p>Cellulose is one of the most abundant natural sources of carbon. In biofuel manufacturing, cellulase is used as an enzyme to hydrolyze cellulose into a fermentable product (glucose)<em>. Pseudomonas stutzeri</em> is one of the microorganisms found in cattle rumen. The microbiome of the rumen is heterogeneous and known for its potentiality to efficiently hydrolyze cellulose. Recent studies have identified, cloned, and crystallized one of the cellulase genes present in <em>P. stutzeri</em>, the A1501 cellulase gene (PST_2494 gene).</p></div><div><h3>Results</h3><p>This study describes the isolation of cellulase-producing bacteria from sheep's rumen. The highest cellulase-producing isolate was identified as <em>P. stutzeri</em> by 16s rDNA sequencing<em>.</em> qRT-PCR was used to measure the cellulase gene expression levels, revealing a higher gene expression of the PST_1459 gene (4 folds) compared to PST_2494 genes. Moreover, cellulase productivity was enhanced by UV irradiation mutagenesis.</p></div><div><h3>Conclusions</h3><p>Sheep's rumen bacterial isolates were tested for their cellulase productivity, and the highest was identified as <em>P. stutzeri</em>. An investigation of the cellulase genes of <em>P. stutzeri</em> revealed the presence of an unidentified cellulase gene (PST_1459). A qRT-PCR reaction was carried out to validate and measure the expression levels of different cellulase genes, revealing a higher gene expression of the PST_1459 gene than PST_2494 genes. Moreover, UV irradiation mutagenesis was performed to enhance cellulase productivity. The gene expression tested by qRT-PCR confirmed the enhancement of cellulase productivity in some of the mutants obtained.</p><p><strong>How to cite:</strong> Al Makishah NH, Elfarash AE. Molecular characterization of cellulase genes in <em>Pseudomonas stutzeri</em>. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.07.004.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"59 ","pages":"Pages 55-61"},"PeriodicalIF":2.7,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000306/pdfft?md5=e246c9613fb6975ebec857eb2e8ff84f&pid=1-s2.0-S0717345822000306-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46172163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lignosus rhinocerus attenuates non-alcoholic fatty liver induced by plant-based high-fat diet in hamster","authors":"Wen-Che Tsai ,&nbsp;Teck-Loong Hoe ,&nbsp;Shao-Chun Lu ,&nbsp;Tzu-Chien Kao ,&nbsp;Hsin-Yi Yin ,&nbsp;Jiunn-Wang Liao ,&nbsp;Hsiao-Wei Wen","doi":"10.1016/j.ejbt.2022.05.004","DOIUrl":"10.1016/j.ejbt.2022.05.004","url":null,"abstract":"<div><h3>Background</h3><p>Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder globally, and is often caused by abnormal fatty acid metabolism. Plant-based fatty acid may lower the risk of NAFLD, but evidence shows that plant-based fatty acid can still induce fatty liver. <em>Lignosus rhinoceros</em> is a mushroom that is widely used as folk medicine in Southeast Asia. Studies indicate that <em>L. rhinoceros</em> can act as an antioxidant and immune modulator, might be a potent modulator for NAFLD. In this study, a plant-based high-fat diet (HFD) was used to induce fatty liver in hamsters. Hot water extract of <em>L. rhinoceros</em> was applied during the induction period. The antioxidant capacity of <em>L. rhinoceros</em> was evaluated.</p></div><div><h3>Results</h3><p>Data reveal that <em>L. rhinoceros</em> extract had promising DPPH scavenging and ferrous ion chelating compacities. The total phenolic and flavonoid contents were 366.23 ± 5.06 mg gallic acid and 28.67 ± 2.5 mg rutin equivalents per gram of raw <em>L. rhinocerus</em> powder, respectively. <em>Lignosus rhinoceros</em> extract lowered body weight, and reduced liver damage which was induced by HFD in hamster, possibly by reducing mononuclear cell infiltration in the liver. <em>Lignosus rhinoceros</em> treatment also lowered the cholesterol content and improved the HDL-c/LDL-c level in hamster serum. Finally, <em>L. rhinoceros</em> reduced the reactive oxygen species content in the liver which was caused by HFD.</p></div><div><h3>Conclusions</h3><p>This investigation provides evidence that a plant-based HFD may be a risk factor in fatty liver. It also proves that <em>L. rhinoceros</em> promotes liver health, and so can be used in functional food.</p><p><strong>How to cite:</strong> Tsai W-C, Hoe T-L, Lu S-C, et al. <em>Lignosus rhinocerus</em> attenuates non-alcoholic fatty liver induced by plant-based high-fat diet in hamster. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.05.004.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 46-54"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000215/pdfft?md5=781688541af6dfb0d7ba2bed04c2c40f&pid=1-s2.0-S0717345822000215-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46518958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved laccase production in Pleurotus djamor RP by atmospheric and room temperature plasma (ARTP) mutagenesis","authors":"Zichen Zhang ,&nbsp;Aabid Manzoor Shah ,&nbsp;Hassan Mohamed ,&nbsp;Yao Zhang ,&nbsp;Beenish Sadaqat ,&nbsp;Nino Tsiklauri ,&nbsp;Tinatin Sadunishvili ,&nbsp;Yuanda Song","doi":"10.1016/j.ejbt.2022.03.005","DOIUrl":"10.1016/j.ejbt.2022.03.005","url":null,"abstract":"<div><h3>Background</h3><p><em>Pleurotus djamor</em> is an edible mushroom with medicinal properties, and degrades lignocellulose by producing enzymes that have a variety of industrial applications. The aim of this study was to improve laccase producing potential of <em>P. djamor</em> that may easily convert lignocellulose waste into high-value products.</p></div><div><h3>Results</h3><p>In the present study, protoplast of the newly isolated fungus <em>P. djamor</em> RP was mutated by atmospheric pressure and room temperature plasma (ARTP) method. Guaiacol was used as an indicator for the rapid visual appearance of laccase positives in the screening process. From the results of preliminary screening, 10 promising positive mutants with a maximum laccase production were selected for further experimentation. Lac activity by the ABTS method showed that the mutant <em>P. djamor</em> strain 51-4 exhibited the maximum laccase production of 494.44 U/L in shake flask cultivation, which was 86.36% higher than wild strain. Notably, the other mutant <em>P. djamor</em> strain 62-27 exhibits a higher laccase production of 26.06 U/g which was 30.28% higher than wild strain when grown on solid-state fermentation. In addition to a higher yield of laccase, all mutants showed better growth and genetic stability than the wild strain.</p></div><div><h3>Conclusions</h3><p><em>P. djamor</em> RP mutants with high laccase-producing potential were developed by ARTP Mutagenesis technique, and these isolates could be used as a possible candidate for lignocellulose conversion into high-value products.</p><p><strong>How to cite</strong>: Zhang Z, Shah AM, Mohamed H, et al. Improved laccase production in <em>Pleurotus djamor</em> RP by atmospheric and room temperature plasma (ARTP) mutagenesis. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.03.005</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 1-9"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000112/pdfft?md5=4b23e1cad962a9fe9ba2995f1e234f58&pid=1-s2.0-S0717345822000112-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43061258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A meta-analysis of low temperature tolerance QTL in maize","authors":"Tao Yu,&nbsp;Jianguo Zhang,&nbsp;Jingsheng Cao,&nbsp;Shiliang Cao,&nbsp;Wenyue Li,&nbsp;Gengbin Yang","doi":"10.1016/j.ejbt.2022.05.002","DOIUrl":"10.1016/j.ejbt.2022.05.002","url":null,"abstract":"<div><h3>Background</h3><p>Cold injury is one of the most important limiting factors for maize production in mid-high latitude regions in the world. A total of 314 QTLs for maize low temperature tolerance have been identified in different populations using different statistical methods. However, few identical QTLs have been identified in different research studies.</p></div><div><h3>Results</h3><p>A consensus map of QTLs related to maize low temperature tolerance was constructed, based on the public genetic map, IBM2 2008 Neighbors as a reference map, along with a set of 314 QTLs reported in the literature over the past 20 years. A total of 187 QTLs were projected onto the IBM2 2008 Neighbors by software BioMercator. Forty-seven consensus QTLs were detected. The confidence interval at all sites ranged from 0.04 cM to 102.73 cM, and the proportion of the phenotypic variance associated with each of them ranged from 3.32% to 20.11%. Major chromosomal sites were identified on Chr.6 (MQTL29, MQTL30, and MQTL31).</p></div><div><h3>Conclusions</h3><p>This study provides further insights into the genetic basis of maize low temperature tolerance. Moreover, the MQTLs reported here could be harnessed for functional marker development and candidate gene mining of maize low temperature tolerance.</p><p><strong>How to cite:</strong> Yu T, Zhang J, Cao J, et al. A meta-analysis of low temperature tolerance QTL in maize. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.05.002</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 82-91"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000197/pdfft?md5=c63f4414cacd3512b28f6f290cd91ee6&pid=1-s2.0-S0717345822000197-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48753705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}