Electronic Journal of Biotechnology最新文献

{"title":"RNA-seq analysis and transcriptome assembly of Salicornia neei reveals a powerful system for ammonium detoxification","authors":"Mónica Díaz-Silva ,&nbsp;Jonathan Maldonado ,&nbsp;Pamela Veloso ,&nbsp;Nicol Delgado ,&nbsp;Herman Silva ,&nbsp;José A. Gallardo","doi":"10.1016/j.ejbt.2022.05.003","DOIUrl":"https://doi.org/10.1016/j.ejbt.2022.05.003","url":null,"abstract":"<div><h3>Background</h3><p><em>Salicornia neei</em> is a halophyte plant that has been proposed for use in the phytoremediation of the saline wastewater generated by land-based aquaculture. To identify the molecular mechanisms related to ammonium response, we analyzed the transcriptome of <em>S. neei</em> in response to growth in saline water containing 3 mM ammonium.</p></div><div><h3>Results</h3><p>The RNA sequencing generated a total of 14,680,108 paired-end reads from the control and stressed conditions. De novo assembly using the CLC Genomic Workbench produced 86,020 transcripts and a reference transcriptome with an N50 of 683 base pair.</p><p>A total of 45,327 genes were annotated, representing 51.2% of the contig predicted from de novo assembly. As regards differentially expressed genes, a total of 9,140 genes were differentially expressed in response to ammonium in saline water; of these, 7,396 could be annotated against functional databases. The upregulated genes were mainly involved in cell wall biosynthesis, transmembrane transport and antiporter activities, including biological Kyoto Encyclopedia of Genes and Genomes, pathways linked to the biosynthesis of secondary metabolites, plant hormone signal transduction, autophagy, and nitrogen metabolism. In addition, a set of 72 genes was directly involved in ammonium metabolism, including glutamine synthetase 1, glutamate synthase 1, and ferredoxin-dependent glutamate synthase chloroplastic.</p></div><div><h3>Conclusions</h3><p>Our results support the hypothesis that an ammonium detoxification system mediated by glutamine and glutamate synthase was activated in <em>S. neei</em> when exposed to ammonium and saline water. The present transcriptome profiling method could be useful when investigating the response of halophyte plants to saline wastewater from land-based aquaculture.</p><p><strong>This manuscript includes an interactive 360 degree video</strong>, supplementary to the materials and methods section. To view the video correctly, it is necessary to scroll through the screen to navigate across the laboratory where you will find 6 interactive points. For an immersive experience a head-mounted display can be used. <strong>Please, visit this URL:</strong> <span>http://ejbiotechnology.info/public/360view/2022/VTPGALLARDO_1v3/index.htm</span><svg><path></path></svg>.</p><p><strong>How to cite:</strong> Díaz-Silva M, Maldonado J, Veloso P, et al. RNA-seq analysis and transcriptome assembly of <em>Salicornia neei</em> reveals a powerful system for ammonium detoxification. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.05.003</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 70-81"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000203/pdfft?md5=f27b8fa0d20e34dd8ad7611e59dbf5d7&pid=1-s2.0-S0717345822000203-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137371699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal transcriptome analysis provides molecular insights into flower development in red-flesh pitaya","authors":"Zhijiang Wu ,&nbsp;Lifang Huang ,&nbsp;Fengzhu Huang,&nbsp;Guifeng Lu,&nbsp;Shuotong Wei,&nbsp;Chaoan Liu,&nbsp;Haiyan Deng,&nbsp;Guidong Liang","doi":"10.1016/j.ejbt.2022.05.005","DOIUrl":"10.1016/j.ejbt.2022.05.005","url":null,"abstract":"<div><h3>Background</h3><p>Pitaya is an important economic fruit worldwide due to its numerous health benefits. A systematic understanding of the mechanism underlying flower development is essential to obtain higher fruit yield and quality. However, the genetic mechanism of flower development is not yet investigated in pitaya. Herein, a transcriptome analysis was performed to determine the transcriptional changes during flower development in red-flesh pitaya by utilizing nine different stages of flower development.</p></div><div><h3>Result</h3><p>A total of 95,412 unigenes were generated with a mean length of 913 nt, and N50 value of 1878 nt. Comparative transcriptomic analysis showed many differentially expressed genes (DEGs) among the flower growth stages. Furthermore, an array of key DEGs were enriched in hormone signaling, transcription, carbohydrate transport, and energy production pathways. In particular, indole-3-acetic acid, abscisic acid, ethylene-responsive transcription factor, constans-like, teosinte branched 1/cycloidea/proliferating cell nuclear antigen factor, apetala1-like, agamous-like MADS-box protein, sepallata, growth-regulating factor, putative axial regulator yabby, leafy/floricaula homolog, and <em>MYB</em> gene-associated transcription factors displayed altered expression, suggesting their critical roles in floral organ development of pitaya. Besides, genes related to sugar synthesis, transportation, and utilization mediate flower growth regulation in pitaya. Eleven genes were selected to perform qRT-PCR analysis to verify the results of the RNA sequencing.</p></div><div><h3>Conclusions</h3><p>Our results provide important insights into the transcriptional regulation of pitaya flower development. These data resources will be a cornerstone for future research that aims at exploring the genetic control of flower development in pitaya.</p><p><strong>How to cite:</strong> Wu Z, Huang L, Huang F, et al. Temporal transcriptome analysis provides molecular insights into flower development in red-flesh pitaya. Electron J Biotechnol 2022;58 https://doi.org/10.1016/j.ejbt.2022.05.005</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 55-69"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000227/pdfft?md5=8a3eda6869111f20e14efc9da2ab7527&pid=1-s2.0-S0717345822000227-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44201856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radical scavenging potency, HPLC profiling and phylogenetic analysis of endophytic fungi isolated from selected medicinal plants of Saudi Arabia","authors":"Abdallah M.A. Hassane ,&nbsp;Taher M. Taha ,&nbsp;Mohamed F. Awad ,&nbsp;Hassan Mohamed ,&nbsp;Mohammad Melebari","doi":"10.1016/j.ejbt.2022.05.001","DOIUrl":"10.1016/j.ejbt.2022.05.001","url":null,"abstract":"<div><h3>Background</h3><p>Endophytic fungi are associated within plant tissues without any harmful effects. They produce a diversity of metabolites and are well known as rich source of bioactive substances. This study aimed to identify fungal isolates from different medicinal plants, evaluate their antioxidant potency and detect components responsible for antioxidant activity.</p></div><div><h3>Results</h3><p>In the present investigation, 4 endophytic fungal isolates were obtained from different medicinal plants <em>Rumex nervosus</em>, <em>Pulicaria crispa</em> and <em>Withania somnifera</em>. Those isolates were characterized as <em>Penicilluim commune, Penicilluim glaucoroseum, Aspergillus flavipes</em> and <em>Fusarium chlamydosporum</em> according to morphological and phylogenetic bases<em>.</em> These strains were propagated on humified rice and their crude ethyl acetate extracts were assayed for their total phenols, flavonoids and antioxidant potency against DPPH free radicals. The results showed that the elevated constituent of phenolics was found in <em>P. commune</em> (148.24 mg/g), followed by <em>F. chlamydosporum</em> (124.25 mg/g), while the highest total flavonoids were determined in <em>A. flavipes</em>, followed by <em>F. chlamydosporum, P. glaucoroseum, P. commune,</em> respectively. Moreover, the maximum hydroxyl radical-scavenging assay was IC₅₀ = 2.13 mg/mL exhibited by <em>P. commune</em>, followed by <em>F. chlamydosporum</em> (2.68 mg/mL). Weak antimicrobial and cytotoxic effects were reported by the studied strains against pathogenic isolates and (HepG2) hepatocellular carcinoma cell line. The fungal EtOAc extracts were characterized quantitatively by the incidence of different phenols, flavonoids, and phenolic acids, as detected by HPLC.</p></div><div><h3>Conclusions</h3><p>Endophytic fungi derived from medicinal plants are a promising candidate for the wide industrial production of antioxidant agents with several useful medical and pharmaceutical applications.</p><p><strong>How to cite:</strong> Hassane A. M. A, Taha T. M, Awad M. F, et al. Radical scavenging potency, HPLC profiling and phylogenetic analysis of endophytic fungi isolated from selected medicinal plants of Saudi Arabia. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.05.001</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 37-45"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000185/pdfft?md5=4f851b7954ea67ddfa97583a1c086101&pid=1-s2.0-S0717345822000185-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43360355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of miR-141-3p over-expression in ovarian cancer","authors":"Lin Shi ,&nbsp;Hao-Jia Sun ,&nbsp;Jing-Jing Zeng ,&nbsp;Zi-Qian Liang ,&nbsp;Yun-Hua Lin ,&nbsp;Su-Ning Huang ,&nbsp;Jiang-Hui Zeng ,&nbsp;Li Yang ,&nbsp;Hao Chen ,&nbsp;Jie Luo ,&nbsp;Kang-Lai Wei","doi":"10.1016/j.ejbt.2022.04.006","DOIUrl":"10.1016/j.ejbt.2022.04.006","url":null,"abstract":"<div><h3>Background</h3><p>The expression of miR-141-3p in many malignant tumors has been verified. Nevertheless, the relationship between ovarian cancer and miR-141-3p remains undetermined. Therefore, further exploration is required.</p></div><div><h3>Results</h3><p>According to data from 100 samples, the final results of RT-qPCR showed that miR-141-3p was highly expressed in ovarian cancer. Furthermore, miR-141-3p was able to distinguish ovarian cancer cells from ovary tissues. The most significant Kyoto Encyclopedia of Genes and Genomes pathway, was regulation of lipolysis in adipocytes in ovarian cancer. The expression of PIK3R1 was negatively correlated with miR-141- 3p. PIK3R1 has a combing site with miR-141-3p.</p></div><div><h3>Conclusions</h3><p>This study examined the expression levels and mechanism of miR-141-3p in ovarian cancer for the first time. The results suggested that miR-141-3p may promote the occurrence of ovarian cancer by down-regulating PIK3R1.</p><p><strong>How to cite:</strong> Shi L, Sun H-J, Zeng J-J, et al. Evaluation of miR-141-3p over-expression in ovarian cancer. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.04.006</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 14-24"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000173/pdfft?md5=d2d49ce0b2037396953cc02d7dee69ea&pid=1-s2.0-S0717345822000173-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41608766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of solid wastes from the walnut industry: Extraction conditions to evaluate the antioxidant and bioherbicidal activities","authors":"Carmen Soto-Maldonado ,&nbsp;Eduardo Caballero-Valdés ,&nbsp;Julissa Santis-Bernal ,&nbsp;John Jara-Quezada ,&nbsp;Lida Fuentes-Viveros ,&nbsp;María Elvira Zúñiga-Hansen","doi":"10.1016/j.ejbt.2022.04.005","DOIUrl":"10.1016/j.ejbt.2022.04.005","url":null,"abstract":"<div><h3>Background</h3><p>Walnut production (<em>Juglans regia</em>) generates a large amount of waste, mainly comprised of husk and shell. The two by-products have several bioactive compounds, mainly phenolic compounds with antioxidant activity. Given the above, this work seeks to establish productive and adequate conditions for the recovery of compounds with antioxidant activity (juglone among them) from such discards to use the extract as a bioherbicide.</p></div><div><h3>Results</h3><p>Temperature and extraction solvent (type of solvent and use of hydroalcoholic mixtures) are relevant factors on phenolic compounds’ (TPC, Folin-Ciocalteu method) recovery and antioxidant activity (AA, DPPH method), observing values from 2 to 17 mg GAE/g shell and 0.5 and 23 mg GAE/g husk, and 3 to 28 mg TE/g shell and 0.2–36 mg TE/g husk for TPC and AA, respectively. Using 50°C and ethanol as extraction solvent, 14,000 µmol TE/100 g of ORAC activity was obtained for both shell and husk. This value increased when a hydroalcoholic mixture was used. Juglone was recovered only from the husk at 166 mg/100 g. The bioherbicidal potential of the extract was evaluated; a phytotoxic effect and a lower plantar density when applying the product to broad-leaf weeds were observed.</p></div><div><h3>Conclusions</h3><p>In conclusion, the walnut process’s residues have a high potential to be used under the circular economy concept in the agri-food sector by obtaining products with high added value.</p><p><strong>How to cite</strong>: Soto-Maldonado C, Caballero-Valdés E, Santis-Bernal J, et al. Potential of solid wastes from the walnut industry: Extraction conditions to evaluate the antioxidant and bioherbicidal activities. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.04.005</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"58 ","pages":"Pages 25-36"},"PeriodicalIF":2.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000161/pdfft?md5=c80f9263c40ec90796120c7b28782d29&pid=1-s2.0-S0717345822000161-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43367837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel antibacterial activity of Sargassum fusiforme extract against coral white band disease","authors":"Nedaa Ahmed ,&nbsp;Hala F. Mohamed ,&nbsp;Changan Xu ,&nbsp;Xiaohui Sun ,&nbsp;Lingfeng Huang","doi":"10.1016/j.ejbt.2022.03.002","DOIUrl":"10.1016/j.ejbt.2022.03.002","url":null,"abstract":"<div><h3>Background</h3><p>Coral diseases are one of the serious threats embroiling in the imbalance of the coral holobiont integrity through disruption of the complex symbiotic relationship between endobiotic alga, coral animal, and a group of microorganisms. Such diseases are usually associated with many bacterial pathogens inflicting gross lesions in corals which show resistance against antibiotics. Therefore, this has led scientists to draw more attention towards the curative compounds from natural resources like herbal plants and seaweeds as substitutes for chemical antimicrobial agents. This study aimed to evaluate the antibacterial activity of the crude extracts (n-hexane, ethyl acetate, and ethanol), alkaloids, and flavonoids from <em>Sargassum fusiforme</em> through Well Diffusion Assay against different isolated bacterial coral pathogens such as (<em>Vibrio owensii, Empedobacter brevis, Providencia vermicola,</em> and <em>Brevibacterium linens</em>) which cause white band disease to coral reef <em>Porites lutea.</em> This study was also validated by bacterial growth kinetics using optical density, dry weight, and plate count method for the isolated coral bacterial pathogens.</p></div><div><h3>Results</h3><p>The results indicated that the crude extract with n-hexane and alkaloid extract showed prominent inhibiting activity against the tested bacterial pathogens compared to other extracts.</p></div><div><h3>Conclusions</h3><p>Here we report <em>S. fusiforme</em> extracts as a novel antibacterial agent against four <em>Porites lutea</em> bacterial pathogens and further investigation is recommended against other coral pathogens. Overall, <em>S. fusiforme</em> extracts might be able to improve the health status of commercially important coral species.</p><p><strong>How to cite:</strong> Ahmed N, Mohamed HF, Xu C, et al. Novel antibacterial activity of <em>Sargassum fusiforme</em> extract against coral white band disease. Electron J Biotechnol 2022;57. https://doi.org/10.1016/j.ejbt.2022.03.002.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"57 ","pages":"Pages 12-23"},"PeriodicalIF":2.7,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000082/pdfft?md5=b5a25228826b3f884bbecbb1b2a3191b&pid=1-s2.0-S0717345822000082-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44419974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sound affects the neuronal maturation of neuroblastoma cells and the repair of damaged tissues","authors":"Hyunjin Cho ,&nbsp;Hee-Jung Park ,&nbsp;Ju-Hye Choi ,&nbsp;Myeong-Hyun Nam ,&nbsp;Jong-Seob Jeong ,&nbsp;Young-Kwon Seo","doi":"10.1016/j.ejbt.2022.03.001","DOIUrl":"10.1016/j.ejbt.2022.03.001","url":null,"abstract":"<div><h3>Background</h3><p>Sound is a kind of mechanical stimulus and has various effects on the growth and metabolism of plants and animal cells. In previous studies, it was confirmed that sound stimulation promotes the neurodifferentiation process of mesenchymal stem cells. In this study, we examined the effect of sound on the maturation of neuroblastoma cells, SH-SY5Y cells, and investigated its effect on an ischemic mouse stroke model. In the <em>in vitro</em> study, SH-SY5Y cells were exposed to the sound for 3 days and then performed rt-PCR, FACS, and western blot for analysis. In the <em>in vivo</em> study, mesenchymal stem cells were injected into the injured area, and then rats were exposed to sound for 4 weeks. Then, immunohistochemical staining and western blotting were performed.</p></div><div><h3>Results</h3><p>Sound upregulated the expression of presynaptic proteins synaptophysin and postsynaptic density protein 95, as well as neuronal-related proteins such as NFL, Tau, and MAP2. T-type calcium channels such as <em>CACNA1G</em> and <em>CACNA1I</em> were also induced by sound. In an experiment using the brain of ischemic mice, the expression of proteins involved in neuronal differentiation such as MAP2, NF200, and S100 was increased, while the inflammation-related proteins IFNγ, MMP9, and TNFα were decreased. In this neuronal differentiation process, both ERK and CREB, which are proteins involved in the initial signal transduction process, were activated.</p></div><div><h3>Conclusions</h3><p>Our study demonstrates that sound, with the advantage of being non-invasive and easy to use, is an effective stimulus that induces neural differentiation and maturation on animal cells.</p><p><strong>How to cite:</strong> Cho H, Park H-J, Choi J-H, et al. Sound affects the neuronal maturation of neuroblastoma cells and the repair of damaged tissues. Electron J Biotechnol 2022;57. https://doi.org/10.1016/j.ejbt.2022.03.001</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"57 ","pages":"Pages 1-11"},"PeriodicalIF":2.7,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000070/pdfft?md5=751788385f419af044208ef06f2ecd02&pid=1-s2.0-S0717345822000070-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45250726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Storage quality and antioxidant properties of yogurt fortified with polyphenol extract from nutmeg, black pepper, and white pepper","authors":"Amal Bakr Shori","doi":"10.1016/j.ejbt.2022.03.003","DOIUrl":"https://doi.org/10.1016/j.ejbt.2022.03.003","url":null,"abstract":"<div><h3>Background</h3><p>The consumption of antioxidant-rich foods can lower the risk of chronic diseases. In the present work, plain yogurt (control) and three kinds of herbal yogurts fortified with a polyphenol extract from nutmeg, black pepper, and white pepper were prepared. Post-acidification, proteolysis, total phenolic content (TPC), and antioxidant activity of yogurt samples were studied over 21 days. Additionally, all yogurt samples were subjected to sensory evaluations on the first day of storage.</p></div><div><h3>Results</h3><p>Higher production (<em>p</em> &lt; 0.05) of lactic acids was observed in all herbal yogurts than in plain yogurt over 21 days of storage. Black pepper and nutmeg enhanced (<em>p</em> &lt; 0.05) the proteolytic activity of yogurt as compared to control, with the maximum values at 21 days. All three herbal yogurts showed the highest TPC (48–55 µg GAE/ml) at day 14 of storage. The radical scavenging activity of yogurt was positively affected (<em>p</em> &lt; 0.05) by the presence of the three kinds of herbal extracts (72–83%; day 0), with the highest values observed in nutmeg yogurt. Ferric reducing antioxidant potential (FRAP) value of nutmeg yogurt was the highest (<em>p</em> &lt; 0.05) among the other samples at day 7 of storage. The ferrous ion chelating (FIC) ability of all the three herbal yogurts ranged from 80% to 83% during 2 weeks. The highest overall preference scores among the three kinds of herbal yogurts were recorded for black pepper yogurt.</p></div><div><h3>Conclusions</h3><p>Polyphenol compounds derived from nutmeg, black pepper, and white pepper extracts could enrich yogurt with antioxidant properties, with nutmeg yogurt showing the best effect.</p><p><strong>How to cite:</strong> Shori AB. Storage quality and antioxidant properties of yogurt fortified with polyphenol extract from nutmeg, black pepper, and white pepper. Electron J Biotechnol 2022;57. https://doi.org/10.1016/j.ejbt.2022.03.003</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"57 ","pages":"Pages 24-30"},"PeriodicalIF":2.7,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000094/pdfft?md5=888cea2eb8659112f8f96269cb41cec4&pid=1-s2.0-S0717345822000094-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136980110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactive 360° video to “Polyphenolic extracts of walnut (Juglans regia) green husk containing juglone inhibit the growth of HL-60 cells and induce apoptosis”","authors":"Carmen Soto-Maldonado ,&nbsp;Mauricio Vergara-Castro ,&nbsp;John Jara-Quezada ,&nbsp;Eduardo Caballero-Valdés ,&nbsp;Andrea Müller-Pavez ,&nbsp;María Elvira Zúñiga-Hansen ,&nbsp;Claudia Altamirano","doi":"10.1016/j.ejbt.2022.04.002","DOIUrl":"10.1016/j.ejbt.2022.04.002","url":null,"abstract":"<div><h3>Background</h3><p>Juglone is a naphthoquinone currently obtained by chemical synthesis with biological activities including antitumor activity. Additionally, juglone is present in the green husk of walnut, which suggests evaluating the effect of GH extracts on carcinogenic cell lines.</p></div><div><h3>Results</h3><p>Walnut green husk ethanolic extract was obtained as 169.1 mg juglone/100 g Green Husk and antioxidant activity (ORAC) of 44,920 μmol Trolox Equivalent/100 g DW Green Husk. At 1 μM juglone in HL-60 cell culture, green husk extract showed an antiproliferative effect, but pure juglone did not; under these conditions, normal fibroblast cells were not affected. A dose-dependent effect on mitochondrial membrane potential loss was observed. Apoptosis of HL-60 was detected at 10 μM juglone. Despite high ORAC values, neither purified juglone nor the extract showed protective effects on HL-60 cells under oxidative conditions.</p></div><div><h3>Conclusions</h3><p>Green husk extract generates an antiproliferative effect in HL-60 cells, which is related to an induction of the early stages of apoptosis and a loss of mitochondrial membrane potential. The normal cells were not affected when juglone is present at concentrations of 1 μM, while at higher concentrations, there is loss of viability of both cancerous and healthy cells.</p><p><strong>How to cite:</strong> Soto-Maldonado C, Vergara-Castro M, Jara-Quezada J, et al. Interactive 360° video to: Polyphenolic extracts of walnut (<em>Juglans regia</em>) green husk containing juglone inhibit the growth of HL-60 cells and induce apoptosis. Electron J Biotechnol 2022;57. <span>https://doi.org/10.1016/j.ejbt.2022.04.002</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"57 ","pages":"Pages 52-53"},"PeriodicalIF":2.7,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000136/pdfft?md5=ec40d1b8158b7fc1fe34030f70ea0403&pid=1-s2.0-S0717345822000136-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44191953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactive 360° video to “Survival of microencapsulated Lactobacillus casei (prepared by vibration technology) in fruit juice during cold storage”","authors":"Araceli Olivares ,&nbsp;Carmen Soto ,&nbsp;Eduardo Caballero ,&nbsp;Claudia Altamirano","doi":"10.1016/j.ejbt.2022.04.003","DOIUrl":"10.1016/j.ejbt.2022.04.003","url":null,"abstract":"<div><h3>Background</h3><p>Foods including probiotics are considered “functional foods.” As an alternative to dairy products, we investigated the behavior of <em>Lactobacillus casei</em> when exposed to low-pH fruit juice. Juices of fruits such as pineapple, raspberry, and orange were assessed. Free and microencapsulated forms of <em>L. casei</em> were compared, and the viability of the probiotic was evaluated under storage at 4°C for 28 d. Microbiological analyses were carried out to ensure a safe and healthy product for consumers who look for foods with probiotics from sources other than dairy.</p></div><div><h3>Results</h3><p>Low pH affected <em>L. casei</em> survival during storage depending on the type of fruit juice. In the case of pineapple juice, some microcapsules were broken, but microcapsules recovered at the end of the storage period had 100% viability (2.3 × 10⁷ CFU/g spheres). In the case of orange juice, more than 91% viability (5.5 × 10⁶ CFU/g spheres) was found. In raspberry juice, viability decreased rapidly, disappearing at the end of the storage period, which was caused by the absorption of high concentrations of anthocyanin inside microcapsules more than low pH.</p></div><div><h3>Conclusions</h3><p>Low pH affected the survival of <em>L. casei</em> under refrigeration; even when they were microencapsulated, acidic conditions impacted their viability. Although pH affects viability, its value is very sensitive and will depend on the type of fruit juice and its composition. Some fruit juices contain compounds used as substrates for Lactobacillus and other compounds with antimicrobial effects.</p><p><strong>How to cite:</strong> Olivares A, Soto C, Caballero E, et al. Interactive 360° video to “Survival of microencapsulated <em>Lactobacillus casei</em> (prepared by vibration technology) in fruit juice during cold storage”. Electron J Biotechnol 2022;57. <span>https://doi.org/10.1016/j.ejbt.2022.04.003</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"57 ","pages":"Pages 42-43"},"PeriodicalIF":2.7,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345822000148/pdfft?md5=d6d3e49ec0fc21c80acb38a80bbb1224&pid=1-s2.0-S0717345822000148-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46478965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}