SNORD3A acts as a potential prognostic and therapeutic biomarker in gastric cancer

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology Pub Date : 2023-10-27 DOI:10.1016/j.ejbt.2023.08.004

Qi Wang , Yifan Li , Xiaoqiang Niu , Chengjiang Zhang , Jun Zhang , Jiaqing Cao , Lidong Wu

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引用次数: 0

Abstract

Background

Although SNORD3A has been implicated in cancer progression, its specific roles and underlying mechanisms in gastric cancer (GC) remain poorly understood. We analysed SNORD3A expression using TCGA data and evaluated patient survival via Kaplan‒Meier curves. Additionally, we conducted GO-KEGG enrichment analysis to identify relevant biological processes and signaling pathways, while ssGSEA was used to assess the correlation between SNORD3A and cancer immune infiltrates. Furthermore, we explored the relationship between SNORD3A and immunotherapy response through TIDE. We verified SNORD3A expression using real-time qPCR and assessed cell proliferation, migration, and invasion via CCK8 and Transwell migration and invasion assays.

Results

Our results revealed that SNORD3A was significantly upregulated in GC, with high expression correlating with poor survival. SNORD3A and related genes were primarily enriched in the insulin/insulin-related growth factor signaling pathway. We also observed negative associations between SNORD3A expression and several immune cells, including activated dendritic cells, CD56bright natural killer cells, central memory CD8 T cells, effector memory CD8 T cells, effector memory CD4 T cells, eosinophils, immature dendritic cells, macrophages, mast cells, MDSCs, memory B cells, monocytes, neutrophil cells, plasmacytoid dendritic cells, regulatory T cells, and T follicular helper cells. High SNORD3A expression also correlated with a poorer response to immunotherapy. Finally, inhibition of SNORD3A suppressed cell proliferation, migration, and invasion.

Conclusions

Our findings suggest that SNORD3A plays a catalytic role in the proliferation, migration and invasiveness of GC and may have potential as a diagnostic biomarker and therapeutic target for GC.

How to cite: Wang Q, Li Y, Niu X, et al. SNORD3A acts as a potential prognostic and therapeutic biomarker in gastric cancer. Electron J Biotechnol 2023; 67. https://doi.org/10.1016/j.ejbt.2023.08.004.

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SNORD3A在胃癌中作为潜在的预后和治疗生物标志物

尽管SNORD3A与癌症进展有关，但其在胃癌(GC)中的具体作用和潜在机制尚不清楚。我们使用TCGA数据分析SNORD3A表达，并通过Kaplan-Meier曲线评估患者生存率。此外，我们进行了GO-KEGG富集分析，以确定相关的生物学过程和信号通路，而ssGSEA用于评估SNORD3A与癌症免疫浸润的相关性。此外，我们通过TIDE探讨了SNORD3A与免疫治疗反应的关系。我们使用实时qPCR验证了SNORD3A的表达，并通过CCK8和Transwell迁移和侵袭试验评估了细胞的增殖、迁移和侵袭。结果我们的研究结果显示，SNORD3A在胃癌中显著上调，高表达与生存差相关。SNORD3A及相关基因主要富集于胰岛素/胰岛素相关生长因子信号通路。我们还观察到SNORD3A的表达与几种免疫细胞呈负相关，包括活化的树突状细胞、CD56bright自然杀伤细胞、中枢记忆性CD8 T细胞、效应记忆性CD8 T细胞、效应记忆性CD4 T细胞、嗜酸性粒细胞、未成熟树突状细胞、巨噬细胞、肥大细胞、MDSCs、记忆性B细胞、单核细胞、中性粒细胞、浆细胞样树突状细胞、调节性T细胞和T滤泡辅助细胞。SNORD3A高表达也与免疫治疗反应较差相关。最后，抑制SNORD3A抑制细胞增殖、迁移和侵袭。结论SNORD3A在胃癌的增殖、迁移和侵袭过程中具有催化作用，可能作为胃癌的诊断标志物和治疗靶点。引用方式:王强，李勇，牛鑫，等。SNORD3A在胃癌中作为潜在的预后和治疗生物标志物。电子学报(英文版)2023;67. https://doi.org/10.1016/j.ejbt.2023.08.004。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Electronic Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology. The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th). The following areas are covered in the Journal: • Animal Biotechnology • Biofilms • Bioinformatics • Biomedicine • Biopolicies of International Cooperation • Biosafety • Biotechnology Industry • Biotechnology of Human Disorders • Chemical Engineering • Environmental Biotechnology • Food Biotechnology • Marine Biotechnology • Microbial Biotechnology • Molecular Biology and Genetics •Nanobiotechnology • Omics • Plant Biotechnology • Process Biotechnology • Process Chemistry and Technology • Tissue Engineering