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Sequence Variability of Nine Cytosolic Ascorbate Peroxidases in Polyploid Strawberry 多倍体草莓九种细胞质抗坏血酸过氧化物酶的序列变异
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109041331
Byung-Hyun Lee, J. Jo, W. Chung
{"title":"Sequence Variability of Nine Cytosolic Ascorbate Peroxidases in Polyploid Strawberry","authors":"Byung-Hyun Lee, J. Jo, W. Chung","doi":"10.3109/10425170109041331","DOIUrl":"https://doi.org/10.3109/10425170109041331","url":null,"abstract":"Little is known about differential gene expression at the molecular level in polyploid plants. Here, we describe the molecular analysis of ApxSC (cytosolic ascorbate peroxidase from a polyploid strawberry) genes. Fifty-three cDNAs encoding ApxSC were isolated from a strawberry fruit cDNA library. These clones were categorized (i) into nine homologous (95 to 99 %) gene groups on the basis of their nucleotide sequences and (ii) into four groups of similar (>98%) polypeptides on the basis of their deduced amino acid sequences. Sequence variation among the gene groups was dispersed throughout the gene, while differences among the polypeptide groups were observed only at three amino acid positions (9, 63, and 233). These results imply that the ApxSC genes show co-dominant expression resulting from multiple alleles. This hypothesis is supported by genomic blots and primer extension analyses.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"222 ","pages":"475 - 484"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91461813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
The Gene and Pseudogenes of Cbx3/mHPlγ Cbx3/mHPlγ的基因和假基因
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109080769
David O. Jones, M. Mattei, D. Horsley, I. Cowell, Prim B. Singh
{"title":"The Gene and Pseudogenes of Cbx3/mHPlγ","authors":"David O. Jones, M. Mattei, D. Horsley, I. Cowell, Prim B. Singh","doi":"10.3109/10425170109080769","DOIUrl":"https://doi.org/10.3109/10425170109080769","url":null,"abstract":"The HP1 class of chromobox (Cbx) genes encode an evolutionarily conserved family of proteins involved in the packaging of chromosomal domains into a repressive heterochromatic state. The murine Cbx5, Cbxl and Cbx3 genes encode the three mouse HP1 proteins, mHPlot, -β and -γ respectively. Here, we report the cloning of the mouse Cbx31 HPlγ gene and the chromosomal localisation of Cbx3 and three Cfo3-related pseudogenes. The Cbx3 structural gene is located on mouse Chromosome 6, close to the Hoxa cluster. Two Cbx3 processed pseudogenes are separated by just 300 bp and are arranged in a head-to-tail configuration on Chromosome 13 while a third pseu-dogene is found on mouse Chromosome 4. The genomic intron-exon arrangement of Cbx3 is different from the conserved organisation of three other mammalian HP1 genes, Cbxl (mHPip), CBX3 (hHPly), and Cbx5 (mHPla) in that Cbx3 lacks an intron that is present in the others.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"1 1","pages":"147 - 160"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90170634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Characterization of the Region Encompassing the Human Lysyl Oxidase Locus 人类赖氨酸氧化酶基因座周围区域的特征
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109024996
R. P. Martins, A. Ujfalusi, K. Csiszȧr, S. Krawetz
{"title":"Characterization of the Region Encompassing the Human Lysyl Oxidase Locus","authors":"R. P. Martins, A. Ujfalusi, K. Csiszȧr, S. Krawetz","doi":"10.3109/10425170109024996","DOIUrl":"https://doi.org/10.3109/10425170109024996","url":null,"abstract":"A 46,823 bp region of human chromosome 5q23.1 encompassing the seven-exon lysyl oxidase gene was characterized at the primary sequence level. Approximately 17.4% of this region is comprised of repetitive elements. The gene colocalizes with microsatellite marker D5S467. It is flanked by two candidate nuclear matrix association regions (MARs). The 5′ MAR centered at position 12,500 is of the AT-rich and curved DNA class. This is followed by a large CpG island containing fifty-seven putative regulatory elements which extend from just upstream of exon 1 to intron 2. The larger 3′ MAR, spans position 35,050–39,750 and is characterized by a TG-rich kinked structure that also contains a topoisomerase II binding site. Based on these results model of the transcriptional regulation of the lysy/oxidase gene is presented.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"29 1","pages":"215 - 227"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76234810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Short Communication: Sequencing and Chromosomal Assignment of the Rat Endothelial-derived Lipase Gene (Lipg) 短通信:大鼠内皮源性脂肪酶基因(Lipg)的测序和染色体分配
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109025004
A. Bonné, M. D. den Bieman, H. V. van Lith, Bert F.M. van Zutphen
{"title":"Short Communication: Sequencing and Chromosomal Assignment of the Rat Endothelial-derived Lipase Gene (Lipg)","authors":"A. Bonné, M. D. den Bieman, H. V. van Lith, Bert F.M. van Zutphen","doi":"10.3109/10425170109025004","DOIUrl":"https://doi.org/10.3109/10425170109025004","url":null,"abstract":"Part of the nucleotide sequence of the Lipg gene in the rat was established using primers based on the mRNA sequence described in the mouse. The rat intron sequence served as a template for designing primers for the specific amplification of rat Lipg. A rat-hamster radiation hybrid (RH) panel was used for chromosomal assignment of the rat Lipg gene. The Lipg gene was found to be located on rat chromosome 18 in the vicinity of the marker D18Mitll; a region reported to be homologous with both human and mouse chromosome 18.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"61 1","pages":"285 - 287"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79599003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Molecular Cloning and Sequence Analysis of the Manganese Catalase Gene from Thermoleophilum album NM 嗜热菌锰过氧化氢酶基因的克隆及序列分析
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084467
Krisana Phucharoen, Yuuki Takenaka, T. Shinozawa
{"title":"Molecular Cloning and Sequence Analysis of the Manganese Catalase Gene from Thermoleophilum album NM","authors":"Krisana Phucharoen, Yuuki Takenaka, T. Shinozawa","doi":"10.3109/10425170109084467","DOIUrl":"https://doi.org/10.3109/10425170109084467","url":null,"abstract":"The manganese catalase gene (mnct) from Thermoleophilum album NM, a thermophilic bacterium, was cloned and its nucleotide sequence was analyzed. The gene consists of 885 bp (65.4% GC content) encoding 294 amino acids with a molecular mass of 32,500 Da. The deduced amino acid sequence shows similarities to those of Thermus species strain YS 8-13 (a thermophilic bacterium) and Bacillus halodurans (an alkaliphilic bacterium) with 61 and 54% identities, respectively.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"113 1","pages":"413 - 417"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72878827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Nucleotide Sequence Analysis of a Transforming Gene Isolated from Nasopharyngeal Carcinoma Cell Line CNE2: an Aberrant Human Immunoglobulin Kappa Light Chain Which Lacks Variable Region 鼻咽癌细胞系CNE2:缺乏可变区异常人免疫球蛋白Kappa轻链转化基因的核苷酸序列分析
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084456
Ming Li, Wei Ren, X. Weng, W. Liao, L. Xia, Xiyun Deng, Ya Cao
{"title":"Nucleotide Sequence Analysis of a Transforming Gene Isolated from Nasopharyngeal Carcinoma Cell Line CNE2: an Aberrant Human Immunoglobulin Kappa Light Chain Which Lacks Variable Region","authors":"Ming Li, Wei Ren, X. Weng, W. Liao, L. Xia, Xiyun Deng, Ya Cao","doi":"10.3109/10425170109084456","DOIUrl":"https://doi.org/10.3109/10425170109084456","url":null,"abstract":"A transforming gene, designated Tx, was isolated from a human nasopharyngeal carcinoma (NPC) cell line CNE2 by transfection and molecular cloning techniques. The Tx gene was analyzed using computer-based bioinformatics and compared with the known sequences in EMBL and GenBank databases. We found that Tx contains human immunoglobulin kappa light chain constant region, five intact joining regions J1-J5, five recombination signal sequences and an N-segment besides classic regulatory sequences such as TATA boxes, CAAT boxes, poly A signals, etc. Interestingly, Tx also contains several binding sites for nuclear transcription factors such as NF-KB, NF-IL6, TFIID, etc. In conclusion, there are only several base pairs mutations or deletions compared with normal Ig K JC gDNA fragment. In all, Tx is an aberrant human immunoglobulin kappa light chain that contains the constant region, five joining regions, which lacks the variable regions.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"1 1","pages":"331 - 335"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89325310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Molecular Cloning, Sequencing Analysis and Expression of the Catalase-Peroxidase Gene from Halobacterium Salinarum 盐盐杆菌过氧化氢酶-过氧化物酶基因的克隆、测序分析及表达
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109042049
Shinong Long, M. Salin
{"title":"Molecular Cloning, Sequencing Analysis and Expression of the Catalase-Peroxidase Gene from Halobacterium Salinarum","authors":"Shinong Long, M. Salin","doi":"10.3109/10425170109042049","DOIUrl":"https://doi.org/10.3109/10425170109042049","url":null,"abstract":"The gene encoding catalase-peroxidase was cloned from chromosomal DNA from the Archaea, Halobacterium salinarum. The nucleotide sequence of a 3.5 kb fragment containing the catalase-peroxidase gene and its flanking regions was determined. A 2.16 kb open reading frame was obtained, encoding the enzyme which was comprised of 720 amino acid residues with a calculated molecular weight of 80 kDa. The deduced amino acid sequence of the H. salinarum catalase-peroxidase showed a high degree of identity to other bifunctional catalase-peroxidases. A transcriptional start site was identified 183 bp upstream of the trans-lational start codon. Southern blot analysis indicated that catalase-peroxidase was a single copy gene. The Archaeal catalase-peroxidase gene was expressed in Escherichia coli, and the expressed fusion protein exhibited both catalase and peroxidase activities.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"57 1","pages":"39 - 51"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86578180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Identification of a Gene Cluster for the Mevalonate Pathway in Lactobacillus Helveticus Helveticus乳杆菌甲羟戊酸途径基因簇的鉴定
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109080773
A. Smeds, T. Joutsjoki, A. Palva
{"title":"Identification of a Gene Cluster for the Mevalonate Pathway in Lactobacillus Helveticus","authors":"A. Smeds, T. Joutsjoki, A. Palva","doi":"10.3109/10425170109080773","DOIUrl":"https://doi.org/10.3109/10425170109080773","url":null,"abstract":"Three Lactobacillus helveticus 53/7 genes essential for the biosynthesis of isopentenyl diphosphate and the gene coding for a putative carotenoid biosynthesis protein were for the first time identified from lactic acid bacteria. The deduced amino acid sequences of the mevalonate pathway gene products share significant identity with corresponding proteins of a few gram-positive cocci and Streptomyces species.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"7 1","pages":"187 - 190"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78438773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Molecular Cloning of cDNA Encoding Polypeptide Chain Elongation Factor la from Goldfish (Carassius auratus) 金鱼多肽链延伸因子la cDNA的分子克隆
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084468
M. Tokumoto, Y. Nagahama, T. Tokumoto
{"title":"Molecular Cloning of cDNA Encoding Polypeptide Chain Elongation Factor la from Goldfish (Carassius auratus)","authors":"M. Tokumoto, Y. Nagahama, T. Tokumoto","doi":"10.3109/10425170109084468","DOIUrl":"https://doi.org/10.3109/10425170109084468","url":null,"abstract":"The goldfish homologue of polypeptide chain elongation factor-la (EF-la) isolated from the ovary of the goldfish is described. The deduced amino acid sequence is highly homologous to EF-la from other species. Analysis of its tissue distribution revealed a single 1.7 kilobase message ubiquitous among various tissues.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"46 1","pages":"419 - 424"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82499038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Genomic DNA Sequence and Transcription Factor Binding Sites of Mouse Ninjurin 小鼠忍尿蛋白基因组DNA序列及转录因子结合位点
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084463
Ae Ran Moon, G. Oh, Jae Wha Kim, Y. Cho, I. Choe
{"title":"Genomic DNA Sequence and Transcription Factor Binding Sites of Mouse Ninjurin","authors":"Ae Ran Moon, G. Oh, Jae Wha Kim, Y. Cho, I. Choe","doi":"10.3109/10425170109084463","DOIUrl":"https://doi.org/10.3109/10425170109084463","url":null,"abstract":"The complete genomic DNA sequence of mouse ninjurin gene has been cloned and sequenced by screening a bacterial artificial chromosome (BAC) library of mouse 129/SvJ genomic DNA. The mouse ninjurin gene comprises four exons and the translatable sequences are included in the first three exons. The putative promoter region of the mouse ninjurin gene lacks the consensus “CAAT” or “TATA” sequence. Nonetheless, it has demonstrated the promoter activity in transient transfection experiment using the construct containing putative promoter sequence of mouse ninjurin and reporter gene. The nucleotide sequence of the putative promoter region shows 83% homology with the corresponding DNA sequence of human ninjurin gene that had been previously reported, and reveals a high degree of conservation between the two species. Analysis of the DNA sequence identified the putative promoters and the binding sites for a variety of transcription factors of mouse ninjurin.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"17 1","pages":"385 - 395"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82395399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
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