Sequence Variability of Nine Cytosolic Ascorbate Peroxidases in Polyploid Strawberry

Byung-Hyun Lee, J. Jo, W. Chung
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引用次数: 5

Abstract

Little is known about differential gene expression at the molecular level in polyploid plants. Here, we describe the molecular analysis of ApxSC (cytosolic ascorbate peroxidase from a polyploid strawberry) genes. Fifty-three cDNAs encoding ApxSC were isolated from a strawberry fruit cDNA library. These clones were categorized (i) into nine homologous (95 to 99 %) gene groups on the basis of their nucleotide sequences and (ii) into four groups of similar (>98%) polypeptides on the basis of their deduced amino acid sequences. Sequence variation among the gene groups was dispersed throughout the gene, while differences among the polypeptide groups were observed only at three amino acid positions (9, 63, and 233). These results imply that the ApxSC genes show co-dominant expression resulting from multiple alleles. This hypothesis is supported by genomic blots and primer extension analyses.
多倍体草莓九种细胞质抗坏血酸过氧化物酶的序列变异
多倍体植物分子水平上的差异基因表达尚不清楚。在这里,我们描述了ApxSC(细胞质抗坏血酸过氧化物酶从多倍体草莓)基因的分子分析。从草莓果实cDNA文库中分离到53个编码ApxSC的cDNA。这些克隆根据核苷酸序列可分为9个同源基因群(95% ~ 99%),根据推导出的氨基酸序列可分为4个相似多肽群(>98%)。基因组之间的序列差异分散在整个基因中,而多肽组之间的差异仅在3个氨基酸位置(9、63和233)上观察到。这些结果表明,ApxSC基因表现出多等位基因的共显性表达。这一假设得到了基因组印迹和引物扩展分析的支持。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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