短通信:大鼠内皮源性脂肪酶基因(Lipg)的测序和染色体分配

DNA Sequence Pub Date : 2001-01-01 DOI:10.3109/10425170109025004

A. Bonné, M. D. den Bieman, H. V. van Lith, Bert F.M. van Zutphen

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引用次数: 1

摘要

根据小鼠的mRNA序列，用引物建立了大鼠Lipg基因的部分核苷酸序列。大鼠内含子序列可作为设计大鼠Lipg特异性扩增引物的模板。采用大鼠-仓鼠辐射杂交(RH)模型对大鼠Lipg基因进行染色体定位。Lipg基因位于大鼠18号染色体D18Mitll标记附近;一个据报道与人类和小鼠18号染色体同源的区域。

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Short Communication: Sequencing and Chromosomal Assignment of the Rat Endothelial-derived Lipase Gene (Lipg)

Part of the nucleotide sequence of the Lipg gene in the rat was established using primers based on the mRNA sequence described in the mouse. The rat intron sequence served as a template for designing primers for the specific amplification of rat Lipg. A rat-hamster radiation hybrid (RH) panel was used for chromosomal assignment of the rat Lipg gene. The Lipg gene was found to be located on rat chromosome 18 in the vicinity of the marker D18Mitll; a region reported to be homologous with both human and mouse chromosome 18.

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DNA Sequence

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