DNA Sequence最新文献_第8页

Genetic Divergence in the algT-muc Operon Controlling Alginate Biosynthesis and Response to Environmental Stress in Pseudornonas syringae 丁香假藓控制藻酸盐合成及对环境胁迫响应的algT-muc操纵子的遗传分化

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109047566

L. Keith, C. Bender

引用次数: 12

Genomic Organization of the Murine G Protein (3 Subunit Genes and Related Processed Pseudogenes 小鼠G蛋白3亚基基因及相关加工伪基因的基因组组织

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084458

J. Kitanaka, Xiao‐Bing Wang, N. Kitanaka, C. Hembree, G. Uhl

{"title":"Genomic Organization of the Murine G Protein (3 Subunit Genes and Related Processed Pseudogenes","authors":"J. Kitanaka, Xiao‐Bing Wang, N. Kitanaka, C. Hembree, G. Uhl","doi":"10.3109/10425170109084458","DOIUrl":"https://doi.org/10.3109/10425170109084458","url":null,"abstract":"The functional significance of heterotrimeric guanine nucleotide binding protein (G protein) for the many physiological processes including the molecular mechanisms of drug addiction have been described. In investigating the changes of mRNA expression after acute psychostimulant administration, we previously identified a cDNA encoding a G protein pi subunit (Gpi) that was increased up to four-fold in certain brain regions after administration of psychostimulants. The mouse Gpi gene (the mouse genetic symbol, GNB1) was mapped to chromosome 4, but little was known of its genetic features. To characterize the GNB1 gene further, we have cloned and analyzed the genomic structures of the mouse GNB1 gene and its homologous sequences. The GNB1 gene spans at least 50 kb, and consists of 12 exons and 11 introns. The exon/intron boundaries were determined and found to follow the GT/AG rule. Exons 3-11 encode the Gpi protein, and the exon 2 is an alternative, resulting in putative two splicing variants. Although intron 11 is additional for GNB1 compared with GNB2 and GNB3, the intron positions within the protein coding region of GNB1, GNB2 and GNB3 are identical, suggesting that GNB1 should have diverged from the ancestral gene family earlier than the genes for GNB2 and GNB3. We also found the 5′-truncated processed pseudogenes with 71-89% similarities to GNB1 mRNA sequence, suggesting that the truncated cDNA copies, which have been reverse-transcribed from a processed mRNA for GNB1, might have been integrated into several new locations in the mouse genome.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"39 1","pages":"345 - 354"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79467559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

xln23 from Streptomyces chattanoogensis UAH23 Encodes a Putative Enzyme with Separate Xylanase and Arabinofuranosidase Catalytic Domains 来自链霉菌chattanoogensis的xln23编码一种假定具有木聚糖酶和阿拉伯糖醛酸酶催化结构域的酶

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109080771

A. Hernández, J. Copa-Patiño, J. Soliveri

引用次数: 7

Isolation and Characterisation of the ylmE Homologue of Thermus thermophilus 嗜热热菌ylmE同源物的分离与鉴定

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109041334

S. Spada, Yann Gibert, J. Pembroke, J. Wall

引用次数: 4

cDNA Cloning of Proglucagon from the Stomach and Pancreas of the Dog 犬胃胰高血糖素原cDNA的克隆

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109024999

D. Irwin

{"title":"cDNA Cloning of Proglucagon from the Stomach and Pancreas of the Dog","authors":"D. Irwin","doi":"10.3109/10425170109024999","DOIUrl":"https://doi.org/10.3109/10425170109024999","url":null,"abstract":"In human and rat, tissue-specific proteolytic processing of identical proglucagon precursors yield tissue-specific proglucagon-derived peptides. In contrast, in many non-mammalian vertebrates alternative mRNA splicing yields different proglucagon precursors in different tissues. Thus alternative mRNA splicing, in part, limits the choices of proglucagon-derived peptides that can be produced by proteolytic processing. Stomach proglucagon mRNAs from the rainbow trout and Xenopus laevis were found not to encode the proglucagon-derived peptide glucagon-like peptide 2 (GLP-2). To determine if the absence of GLP-2 was a general feature of stomach proglucagons we isolated and characterized proglucagon cDNAs from the stomach and the pancreas of the dog, a mammal that expresses the proglucagon gene in the stomach. A major proglucagon transcript of about 1100 bases and a minor transcript of about 800 bases were identified in both stomach and pancreas. The coding sequences of both the stomach and pancreatic proglucagon transcripts were identical. Therefore, tissue-specific proteolytic processing, and not alternative mRNA splicing, must regulate the production of tissue-specific proglucagon-derived peptides from the stomach of the dog.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"265 2","pages":"253 - 260"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91456328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Isolation and Sequence Analysis of the Arbuscular Mycorrhizal Fungus Glomus mosseae (Nicol & Gerd.) Gerdemann & Trappe 3-Phosphoglycerate Kinase (PGK) Gene Promoter Region 丛枝菌根真菌Glomus mosseae (Nicol & Gerd.)的分离与序列分析3-磷酸甘油酸激酶(PGK)基因启动子区

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109041330

L. Harrier

引用次数: 13

Cloning of a cDNA Encoding an Isoform of Human Protein Phosphatase Inhibitor 2 from Vascularized Breast Tumor 人蛋白磷酸酶抑制剂2在乳腺血管化肿瘤中异构体cDNA的克隆

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109041335

I. Wu, Marsha A. Mosesx

引用次数: 3

Complete cDNA and Deduced Amino Acid Sequence of the Chaperonin Containing T-Complex Polypeptide 1 (CCT) Delta Subunit from Aedes triseriatus Mosquitoes 三体伊蚊含t -复合物多肽1 (CCT) δ亚基伴侣蛋白完整cDNA及氨基酸序列推断

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109080776

B. Blitvich, A. Rayms-keller, C. Blair, B. Beaty

引用次数: 3

Identification of Two Fas-Associated Phosphatase-1 (FAP-1) Promoters in Human Cancer Cells 人类癌细胞中两种fas相关磷酸酶-1启动子的鉴定

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109041336

S. Irie, Yin Li, H. Kanki, Tomoko Ohyama, L. Deaven, Stefan Someo, Taka-Aki Sato

{"title":"Identification of Two Fas-Associated Phosphatase-1 (FAP-1) Promoters in Human Cancer Cells","authors":"S. Irie, Yin Li, H. Kanki, Tomoko Ohyama, L. Deaven, Stefan Someo, Taka-Aki Sato","doi":"10.3109/10425170109041336","DOIUrl":"https://doi.org/10.3109/10425170109041336","url":null,"abstract":"Fas-associated phosphatase-1 (FAP-1) has been reported as a negative regulator of Fas-mediated signal transduction in human cancer cells. To obtain insights into the potential carcinogenesis of the FAP-1 gene, we investigated its transcriptional regulation in normal and cancerous cells. To identify the FAP-1 promoter sequences, we first isolated PI and cosmid clones that contained the regulatory region upstream from the FAP-1 gene by using the PCR products of 5′ rapid amplification of cDN A end (5′-RACE) as probes. Genomic analysis of positive clones revealed that the major FAP-1 mRNA was transcribed from its proximal promoter (pPRM) in all human cancer cell lines tested, but 1 additional large transcript derived from its distal promoter (dPRM) was found in the human colon cancer cell line DLD-1. This suggests that the FAP-1 gene may be aberrantly dysregulated in some types of human cancers, including colon carcinoma. Sequence analysis of the region upstream from the FAP-1 gene strongly suggests that the transcript of the FAP-1 gene may be controlled by a variety of transcriptional regulatory elements, including NF-κB, NF-IL6, and p53 in its 2 promoters. These results imply that the FAP-1 gene may be a target gene under the control of important apoptosis-related nuclear factors in human cancers.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"51 1","pages":"519 - 526"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80723913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Genomic DNA Sequences Encoding Malus X domestica Borkh. “Akane”, “Delicious” and Malus transitoria S-RNases 家蝇的基因组DNA序列。“茜”，“美味”和短暂的苹果s - rna

DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084462

S. Matsumoto, S. Hayashi, K. Kitahara, J. Soejima

引用次数: 7