Isolation and Sequence Analysis of the Arbuscular Mycorrhizal Fungus Glomus mosseae (Nicol & Gerd.) Gerdemann & Trappe 3-Phosphoglycerate Kinase (PGK) Gene Promoter Region

Abstract

The Glomus mosseae 3-phosphogly cerate kinase (GmPGK) gene promoter has been isolated from a phage genomic library and represents one of the few promoter elements to be isolated and analysed from these symbiotic fungi. The analysis revealed the presence of several motifs which are found in the promoter region of other fungal PGK genes. In particular, DNA sequences homologous to segments of the S. cer-evisiae and Rhizopus niveus upstream activating elements (UAS). The importance of these UAS sequences in regulating carbon source in PGK genes is known and the presence of two carbon source regulated UAS sequences in the GmPGK gene promoter and its role in the biology of AM fungi is discussed briefly.