嗜热菌锰过氧化氢酶基因的克隆及序列分析

DNA Sequence Pub Date : 2001-01-01 DOI:10.3109/10425170109084467

Krisana Phucharoen, Yuuki Takenaka, T. Shinozawa

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引用次数: 4

摘要

从嗜热细菌Thermoleophilum album NM中克隆了锰过氧化氢酶基因(mnct)，并对其核苷酸序列进行了分析。该基因全长885 bp (GC含量65.4%)，编码294个氨基酸，分子量为32,500 Da。所得氨基酸序列与嗜热菌YS 8-13和嗜碱杆菌YS 8-13的氨基酸序列相似，同源性分别为61%和54%。

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Molecular Cloning and Sequence Analysis of the Manganese Catalase Gene from Thermoleophilum album NM

The manganese catalase gene (mnct) from Thermoleophilum album NM, a thermophilic bacterium, was cloned and its nucleotide sequence was analyzed. The gene consists of 885 bp (65.4% GC content) encoding 294 amino acids with a molecular mass of 32,500 Da. The deduced amino acid sequence shows similarities to those of Thermus species strain YS 8-13 (a thermophilic bacterium) and Bacillus halodurans (an alkaliphilic bacterium) with 61 and 54% identities, respectively.

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DNA Sequence

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